耐镉功能内生菌的筛选及其固定化处理含镉废水
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摘要
从芦苇根部筛选出一株耐镉内生细菌,经鉴定为Chryseobacterium rhizosphaerae SH-1;Cd~(2+)对该菌株最小抑菌浓度高达450 mg/L,显微观察发现其细胞表面吸附机制参与了Cd~(2+)的去除过程;FTIR分析表明菌株表面的O-H、N-H、C-H、N≡C-、-NO_2、C=S、P=O、S-O等基团均参与了吸附作用。采用海藻酸钠凝胶包埋法对菌株SH-1进行固定化,考察了吸附时间、pH和Cd~(2+)初始浓度对固定化微生物吸附Cd~(2+)影响,并对其吸附特性进行了动力学研究。结果表明:固定化内生菌SH-1在Cd~(2+)初始浓度为100 mg/L,吸附时间为480 min,溶液pH值为5条件下,对Cd~(2+)的去除率高达93.24%;Langmuir等温吸附模型能描述其吸附Cd~(2+)的等温吸附特征,最大理论吸附量为187.41 mg/g。另一方面,准二级动力学模型较好地拟合了固定化SH-1吸附Cd~(2+)的动力学过程,理论平衡吸附量为51.02 mg/g。这些结果均证实固定化内生菌SH-1是一种很有潜力的生物吸附剂。
A cadmium-resistant endophytic bacterium was isolated from reed rhizosphere and identified as Chryseobacterium rhizosphaerae SH-1. The minimum inhibitory concentration of Cd~(2+ )was up to 450 mg/L, and microscopic observation showed that the cell surface adsorption mechanism was involved in the removal process of Cd~(2+). FTIR analysis showed that O-H, N-H, C-H, N≡C-,-NO_2, C=S, P=O and S-O were all involved in the adsorption. The strain was immobilized using sodium alginate. The effects of contact time, pH and Cd~(2+ )initial concentrations on Cd~(2+) adsorption by immobilized beads were investigated and the adsorption properties were also explored through adsorption kinetics. The result showed that the removal efficiency was over 93.24% while the initial Cd~(2+) concentration of 100 mg/L, contact time of 480 min and pH of 5.0. The isothermal adsorption characteristics of Cd~(2+) could be described by Langmuir isothermic adsorption model with the maximum theoretical adsorption capacity of 187.41 mg/g. On the other hand, the pseudo-second-order model well fitted the kinetic process of Cd~(2+) adsorption by immobilized SH-1 and the theoretical equilibrium adsorption capacity was 51.02 mg/g. The results showed that the immobilized endophytic bacterium SH-1 could be a great potential biosorbent.
A cadmium-resistant endophytic bacterium was isolated from reed rhizosphere and identified as Chryseobacterium rhizosphaerae SH-1. The minimum inhibitory concentration of Cd~(2+ )was up to 450 mg/L, and microscopic observation showed that the cell surface adsorption mechanism was involved in the removal process of Cd~(2+). FTIR analysis showed that O-H, N-H, C-H, N≡C-,-NO_2, C=S, P=O and S-O were all involved in the adsorption. The strain was immobilized using sodium alginate. The effects of contact time, pH and Cd~(2+ )initial concentrations on Cd~(2+) adsorption by immobilized beads were investigated and the adsorption properties were also explored through adsorption kinetics. The result showed that the removal efficiency was over 93.24% while the initial Cd~(2+) concentration of 100 mg/L, contact time of 480 min and pH of 5.0. The isothermal adsorption characteristics of Cd~(2+) could be described by Langmuir isothermic adsorption model with the maximum theoretical adsorption capacity of 187.41 mg/g. On the other hand, the pseudo-second-order model well fitted the kinetic process of Cd~(2+) adsorption by immobilized SH-1 and the theoretical equilibrium adsorption capacity was 51.02 mg/g. The results showed that the immobilized endophytic bacterium SH-1 could be a great potential biosorbent.
引文
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[22]白红娟,张肇铭,李保珍,等.固定化球形红细菌去除镉的动力学及其与质粒的关系[J].应用与环境生物学报,2008,14(2):249-252.
[2]WANG L,YANG X Q,WANG Q,et al.The accumulation of Cd2+ and the effect on EST in five tissues and organs of Eriocheir sinensis[J].Acta Zoologica Sinica,2001,(47):96-100.
[3]GAO M,ZHANG Z,LYU M,et al.Toxic effects of nanomaterial-adsorbed cadmium on Daphnia magna[J].Ecotoxicolgy and Environmental Safety,2018,148(3):261-268.
[4]FENG J,SHI Q,WANG X,et al.Silicon supplementation ameliorated the inhibition of photosynthesis and nitrate metabolism by cadmium (Cd) toxicity in Cucumis sativus L[J].Scientia Horticulturae,2010,123(4):521-530.
[5]RINANTI A,FACHRUL M F,HADISOEBROTO R,et al.Improving biosorption of Cu(II)-ion on artificial wastewater by immobilized biosorbent of tropical microalgae[J].International Journal of Geomate,2017,13(36):6-10.
[6]KACAR Y,?IGDEM A,TAN S,et al.Biosorption of Hg(II) and Cd(II) from aqueous solutions:comparison of biosorptive capacity of alginate and immobilized live and heat inactivated Phanerochaete chrysosporium[J].Process Biochemistry,2002,37(6):601-610.
[7]KWIATKOWSKAMARKS S,WOJCIK M.Removal of cadmium (II) from aqueous solutions by calcium alginate beads[J].Separation Science and Technology,2014,49(14):2204-2211.
[8]WEN X,DU C,ZENG G,et al.A novel biosorbent prepared by immobilized Bacillus licheniformis for lead removal from wastewater[J].Chemosphere,2018,200:173-179.
[9]江慧.海藻酸钠-明胶-PVA包埋法固定化酵母菌吸附重金属研究[D].成都:西南交通大学,2016.
[10]文晓凤,杜春艳,袁瀚宇,等.改性磁性纳米颗粒固定内生菌Bacillus nealsonii吸附废水中Cd2+的特性研究[J].环境科学学报,2016,36(12):4376-4383.
[11]赵忠良,崔秀霞,贾雪艳,等.固定化啤酒废酵母对Cd2+生物吸附性能的研究[J].化学与生物工程,2009,26(3):72-75.
[12]李大军,周立,王庆龙,等.固定化生物吸附剂对废水中Pb2+和Cd2+的吸附性能[J].过程工程学报,2017,17(2):248-253.
[13]GAO W,DENG L,ZHU L,et al.Phytoremediation of cadmium/zinc contaminated agricultural soil by long term application of sewage sludge manure using Sedum plumbizincicola[J].Science and Technology Review,2016,34(2):241-246.
[14]LI Y,ZHU J,ZHAI Z,et al.Endophytic bacterial diversity in roots of Phragmites australis in constructed Beijing Cuihu Wetland (China)[J].FEMS Microbiology Letters,2010,309(1):84-93.
[15]田雪雪,程玉立,张圆圆,等.一株异养硝化-好氧反硝化功能菌的分离鉴定及其脱氮特性[J].环境工程学报,2017,11(2):1269-1275.
[16]YIN Y,HU Y,XIONG F.Sorption of Cu(II) and Cd(II) by extracellular polymeric substances (EPS) from Aspergillus fumigatus[J].International Biodeterioration and Biodegradation,2011,65(7):1012-1018.
[17]TAN W,TING A S.Efficacy and reusability of alginate-immobilized live and heat-inactivated Trichoderma asperellum cells for Cu (II) removal from aqueous solution[J].Bioresource Technology,2012,123(123):290-295.
[18]HO Y S,OFOMAJA A E.Biosorption thermodynamics of cadmium on coconut copra meal as biosorbent[J].Biochemical Engineering Journal,2006,30(2):117-123.
[19]李学龙,刘国丽,李超,等.蜡样芽胞杆菌菌体及其胞外聚合物对Cd2+的吸附作用[J].江苏农业学报,2018,34(2):327-332.
[20]刘杨眉,魏桃员,王欣,等.包埋固定化海洋硅藻吸附材料的制备及其对水中铅离子的吸附特性研究[J].环境科学学报,2017,37(5):1763-1773.
[21]陈亚奎.废水中重金属镉吸附剂的制备与吸附机理研究[D].抚顺:辽宁石油化工大学,2017.
[22]白红娟,张肇铭,李保珍,等.固定化球形红细菌去除镉的动力学及其与质粒的关系[J].应用与环境生物学报,2008,14(2):249-252.
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