四川地方中小叶茶树资源的表型遗传多样性
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摘要
为保护和鉴定四川茶树资源,并进行特异资源的挖掘,对109份四川中小叶茶树资源的30个表型性状进行统计分析和主成分聚类分析。研究表明,四川中小叶茶树资源的变异系数和多样性指数平均分别为32.27%和0.98,茶树资源性状差异明显,多样性较丰富。主成分聚类分析显示,12个主成分因子的累积贡献率为71.14%,将供试材料聚类分为8个类群,且与供试材料的原产地关系较小。四川中小叶茶树资源表型性状遗传多样性较丰富,且各原产地茶树表型性状差异较大,可为四川茶树核心种质库的构建和茶树良种选育提供参考。
In order to protect and identify tea resources, and excavate special resources in Sichuan Province, principal component analysis(PCA) and cluster analysis were carried out on 30 phenotypic traits of 109 " Small & Medium Leaf" tea resources in Sichuan. The results showed that the variation coefficient and diversity index of "Small & Medium Leaf" tea resources in Sichuan Province were 32.27% and 0.98, respectively. There were obvious differences in phenotypic traits of tea resources and rich diversity. Principal component analysis(PCA) and cluster analysis showed that the cumulative contribution rate of 12 principal component factors was 71.14%. The materials which were tested were clustered into 8 groups, and had little correlation with their provenance. The "Small & Medium Leaf" tea resources in Sichuan Province had rich phenotypic-traits genetic diversity, and phenotypic traits of different-provenance teas were different, which provided references for structure of core germplasm bank of Sichuan tea plants and selection of tea varieties.
In order to protect and identify tea resources, and excavate special resources in Sichuan Province, principal component analysis(PCA) and cluster analysis were carried out on 30 phenotypic traits of 109 " Small & Medium Leaf" tea resources in Sichuan. The results showed that the variation coefficient and diversity index of "Small & Medium Leaf" tea resources in Sichuan Province were 32.27% and 0.98, respectively. There were obvious differences in phenotypic traits of tea resources and rich diversity. Principal component analysis(PCA) and cluster analysis showed that the cumulative contribution rate of 12 principal component factors was 71.14%. The materials which were tested were clustered into 8 groups, and had little correlation with their provenance. The "Small & Medium Leaf" tea resources in Sichuan Province had rich phenotypic-traits genetic diversity, and phenotypic traits of different-provenance teas were different, which provided references for structure of core germplasm bank of Sichuan tea plants and selection of tea varieties.
引文
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[7] 周波,江海东,张秀新,等.部分引进牡丹品种的形态多样性[J].生物多样性,2011,19(5):543-550.ZHOU B,JIANG H D,ZHANG X X,et al.Morphological diversity of some introduced tree peony cultivars[J].Biodiversity Science,2011,19(5):543-550.(in Chinese with English abstract)
[8] LIU S R,AN Y L,LI F D,et al.Genome-wide identification of simple sequence repeats and development of polymorphic SSR markers for genetic studies in tea plant (Camellia sinensis)[J].Molecular Breeding,2018,38(5):59.
[9] YAO M Z,CHEN L,LIANG Y R.Genetic diversity among tea cultivars from China,Japan and Kenya revealed by ISSR markers and its implication for parental selection in tea breeding programmes[J].Plant Breeding,2008,127(2):166-172.
[10] YAO M Z,MA C L,QIAO T T,et al.Diversity distribution and population structure of tea germplasms in China revealed by EST-SSR markers[J].Tree Genetics & Genomes,2012,8(1):205-220.
[11] ZHAO L P,LIU Z,CHEN L,et al.Generation and characterization of 24 novel EST derived microsatellites from tea plant (Camellia sinensis) and cross-species amplification in its closely related species and varieties[J].Conservation Genetics,2008,9(5):1327-1331.
[12] 蒋会兵,宋维希,矣兵,等.云南茶树种质资源的表型遗传多样性[J].作物学报,2013,39(11):2000-2008.JIANG H B,SONG W X,YI B,et al.Genetic diversity of tea germplasm resources in Yunnan Province based on phenotypic characteristics[J].Acta Agronomica Sinica,2013,39(11):2000-2008.(in Chinese with English abstract)
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[15] 乔玲,陈红霖,王丽侠,等.国外绿豆种质资源农艺性状的遗传多样性分析[J].植物遗传资源学报,2015,16(5):986-993.QIAO L,CHEN H L,WANG L X,et al.Genetic diversity of foreign mungbean germplasm resources by agronomic characters[J].Journal of Plant Genetic Resources,2015,16(5):986-993.(in Chinese with English abstract)
[16] 聂石辉,彭琳,王仙,等.鹰嘴豆种质资源农艺性状遗传多样性分析[J].植物遗传资源学报,2015,16(1):64-70.NIE S H,PENG L,WANG X,et al.Genetic diversity of agronomic traits in chickpea (Cicer arietinum L.) germplasm resources[J].Journal of Plant Genetic Resources,2015,16(1):64-70.(in Chinese with English abstract)
[17] 董承光,王娟,周小凤,等.基于表型性状的陆地棉种质资源遗传多样性分析[J].植物遗传资源学报,2016,17(3):438-446.DONG C G,WANG J,ZHOU X F,et al.Evaluation on genetic diversity of cotton germplasm resources (Gossypium hirsutum L.) on morphological characters[J].Journal of Plant Genetic Resources,2016,17(3):438-446.(in Chinese with English abstract)
[18] 李华锋,滕杰,杨家干,等.连南栽培型古茶树资源叶片表型性状遗传多样性及聚类分析[J].中国农学通报,2016,32(36):109-114.LI H F,TENG J,YANG J G,et al.Genetic diversity and cluster analysis of leaf phenotypic traits of Liannan cultivated ancient tea[J].Chinese Agricultural Science Bulletin,2016,32(36):109-114.(in Chinese with English abstract)
[19] 刘绍杰,迟琳,谢文钢,等.古蔺牛皮茶种质资源遗传多态性[J].作物学报,2014,40(12):2118-2127.LIU S J,CHI L,XIE W G,et al.Genetic diversity of Gulin-niupicha [Camellia sinensis(L.) O.Kuntze] germplasm resources[J].Acta Agronomica Sinica,2014,40(12):2118-2127.(in Chinese with English abstract)
[20] 俞文生,倪佳成,陈慧,等.茶树种质资源表型遗传多样性分析[J].安徽农业科学,2017,45(25):40-42.YU W S,NI J C,CHEN H,et al.Genetic diversity of tea germplasm resources based on phenotypic characteristics[J].Journal of Anhui Agricultural Sciences,2017,45(25):40-42.(in Chinese with English abstract)
[21] 沈程文.广东茶树种质资源遗传多样性的形态和分子评价及其亲缘关系研究[D].长沙:湖南农业大学,2007.SHEN C W.Studies on genetic diversity and phylogenetic relationship of tea [Camellia sinensis (L.) O.Kuntze] germplasm in Guangdong Province by morphological and molecular techniques [D].Changsha:Hunan Agricultural University,2007.(in Chinese with English abstract)
[22] 李慧.南江大叶茶种质资源形态、生化及遗传多样性的分析[D].雅安:四川农业大学,2017.LI H.Analysis on the germ-plasm resources's morphology,biochemistry and genetic diversity of Nanjiang large-leaf tea[D].Ya'an:Sichuan Agricultural University,2017.(in Chinese)
[23] 葛立雯,郭维,潘正康,等.贵州姑菁野生茶树形态多样性及相关性研究[J].植物遗传资源学报,2015,16(3):497-502.GE L W,GUO W,PAN Z K,et al.The morphological diversity and correlation research of Gu Jing wild tea plant from Guizhou[J].Journal of Plant Genetic Resources,2015,16(3):497-502.(in Chinese with English abstract)
[24] 张小琴,周富裕,杨春,等.贵定鸟王种茶树资源农艺性状和品质性状多样性分析[J].分子植物育种,2015,13(2):415-423.ZHANG X Q,ZHOU F Y,YANG C,et al.Diversity of tea germplasm resource (Camellia sinensis ‘Guiding-niaowangzhong’) revealed based on agronomic and quality traits[J].Molecular Plant Breeding,2015,13(2):415-423.(in Chinese with English abstract)
[25] 于晓虎.茄子种质资源形态学标记及ISSR、SSR遗传多样性分析[D].重庆:西南大学,2012.YU X H.Analysis of genetic variation in eggplant using morphological and ISSR,SSR makers[D].Chongqing:Southwest University,2012.(in Chinese with English abstract)
[26] 段志芬,刘本英,汪云刚,等.云南野生茶树资源农艺性状多样性分析[J].西北农业学报,2013,22(1):125-131.DUAN Z F,LIU B Y,WANG Y G,et al.Diversity analysis of agronomic traits of wild tea plants in Yunnan[J].Acta Agriculturae Boreali-Occidentalis Sinica,2013,22(1):125-131.(in Chinese with English abstract)
[2] 罗凡,王云,李春华,等.四川茶树品种研究现状与发展趋势[J].贵州科学,2008,26(2):52-57.LUO F,WANG Y,LI C H,et al.Research actuality and development trend of Sichuan's tea varieties[J].Guizhou Science,2008,26(2):52-57.(in Chinese with English abstract)
[3] TAN L Q,WANG L Y,WEI K,et al.Floral transcriptome sequencing for SSR marker development and linkage map construction in the tea plant (Camellia sinensis)[J].PLoS One,2013,8(11):e81611.
[4] 席春奕.30份四川茶树主要种质资源遗传多样性和亲缘关系的SRAP分析[D].雅安:四川农业大学,2012.XI C Y.Genetic diversity and relationship of 30 tea plant main germplasm in Sichuan revealed by SRAP marker[D].Ya'an:Sichuan Agricultural University,2012.(in Chinese with English abstract)
[5] 刘新龙,马丽,蔡青,等.云南甘蔗品种表型性状的遗传多样性分析[J].植物遗传资源学报,2010,11(6):703-708.LIU X L,MA L,CAI Q,et al.Genetic diversity analysis of phenotypic traits in Yunnan sugarcane varieties[J].Journal of Plant Genetic Resources,2010,11(6):703-708.(in Chinese with English abstract)
[6] 芮文婧,王晓敏,张倩男,等.番茄353份种质资源表型性状遗传多样性分析[J].园艺学报,2018,45(3):561-570.RUI W J,WANG X M,ZHANG Q N,et al.Genetic diversity analysis of 353 tomato germplasm resources by phenotypic traits[J].Acta Horticulturae Sinica,2018,45(3):561-570.(in Chinese with English abstract)
[7] 周波,江海东,张秀新,等.部分引进牡丹品种的形态多样性[J].生物多样性,2011,19(5):543-550.ZHOU B,JIANG H D,ZHANG X X,et al.Morphological diversity of some introduced tree peony cultivars[J].Biodiversity Science,2011,19(5):543-550.(in Chinese with English abstract)
[8] LIU S R,AN Y L,LI F D,et al.Genome-wide identification of simple sequence repeats and development of polymorphic SSR markers for genetic studies in tea plant (Camellia sinensis)[J].Molecular Breeding,2018,38(5):59.
[9] YAO M Z,CHEN L,LIANG Y R.Genetic diversity among tea cultivars from China,Japan and Kenya revealed by ISSR markers and its implication for parental selection in tea breeding programmes[J].Plant Breeding,2008,127(2):166-172.
[10] YAO M Z,MA C L,QIAO T T,et al.Diversity distribution and population structure of tea germplasms in China revealed by EST-SSR markers[J].Tree Genetics & Genomes,2012,8(1):205-220.
[11] ZHAO L P,LIU Z,CHEN L,et al.Generation and characterization of 24 novel EST derived microsatellites from tea plant (Camellia sinensis) and cross-species amplification in its closely related species and varieties[J].Conservation Genetics,2008,9(5):1327-1331.
[12] 蒋会兵,宋维希,矣兵,等.云南茶树种质资源的表型遗传多样性[J].作物学报,2013,39(11):2000-2008.JIANG H B,SONG W X,YI B,et al.Genetic diversity of tea germplasm resources in Yunnan Province based on phenotypic characteristics[J].Acta Agronomica Sinica,2013,39(11):2000-2008.(in Chinese with English abstract)
[13] 中华人民共和国农业农村部.农业作物资源鉴定技术规程茶树:NY/T 1312—2007[S].北京:中国农业出版社,2007
[14] 中华人民共和国农业农村部.植物新品种特异性、一致性和稳定性测试指南茶树:NY/T 2422-2013[S].中国农业出版社,2013:1-14.
[15] 乔玲,陈红霖,王丽侠,等.国外绿豆种质资源农艺性状的遗传多样性分析[J].植物遗传资源学报,2015,16(5):986-993.QIAO L,CHEN H L,WANG L X,et al.Genetic diversity of foreign mungbean germplasm resources by agronomic characters[J].Journal of Plant Genetic Resources,2015,16(5):986-993.(in Chinese with English abstract)
[16] 聂石辉,彭琳,王仙,等.鹰嘴豆种质资源农艺性状遗传多样性分析[J].植物遗传资源学报,2015,16(1):64-70.NIE S H,PENG L,WANG X,et al.Genetic diversity of agronomic traits in chickpea (Cicer arietinum L.) germplasm resources[J].Journal of Plant Genetic Resources,2015,16(1):64-70.(in Chinese with English abstract)
[17] 董承光,王娟,周小凤,等.基于表型性状的陆地棉种质资源遗传多样性分析[J].植物遗传资源学报,2016,17(3):438-446.DONG C G,WANG J,ZHOU X F,et al.Evaluation on genetic diversity of cotton germplasm resources (Gossypium hirsutum L.) on morphological characters[J].Journal of Plant Genetic Resources,2016,17(3):438-446.(in Chinese with English abstract)
[18] 李华锋,滕杰,杨家干,等.连南栽培型古茶树资源叶片表型性状遗传多样性及聚类分析[J].中国农学通报,2016,32(36):109-114.LI H F,TENG J,YANG J G,et al.Genetic diversity and cluster analysis of leaf phenotypic traits of Liannan cultivated ancient tea[J].Chinese Agricultural Science Bulletin,2016,32(36):109-114.(in Chinese with English abstract)
[19] 刘绍杰,迟琳,谢文钢,等.古蔺牛皮茶种质资源遗传多态性[J].作物学报,2014,40(12):2118-2127.LIU S J,CHI L,XIE W G,et al.Genetic diversity of Gulin-niupicha [Camellia sinensis(L.) O.Kuntze] germplasm resources[J].Acta Agronomica Sinica,2014,40(12):2118-2127.(in Chinese with English abstract)
[20] 俞文生,倪佳成,陈慧,等.茶树种质资源表型遗传多样性分析[J].安徽农业科学,2017,45(25):40-42.YU W S,NI J C,CHEN H,et al.Genetic diversity of tea germplasm resources based on phenotypic characteristics[J].Journal of Anhui Agricultural Sciences,2017,45(25):40-42.(in Chinese with English abstract)
[21] 沈程文.广东茶树种质资源遗传多样性的形态和分子评价及其亲缘关系研究[D].长沙:湖南农业大学,2007.SHEN C W.Studies on genetic diversity and phylogenetic relationship of tea [Camellia sinensis (L.) O.Kuntze] germplasm in Guangdong Province by morphological and molecular techniques [D].Changsha:Hunan Agricultural University,2007.(in Chinese with English abstract)
[22] 李慧.南江大叶茶种质资源形态、生化及遗传多样性的分析[D].雅安:四川农业大学,2017.LI H.Analysis on the germ-plasm resources's morphology,biochemistry and genetic diversity of Nanjiang large-leaf tea[D].Ya'an:Sichuan Agricultural University,2017.(in Chinese)
[23] 葛立雯,郭维,潘正康,等.贵州姑菁野生茶树形态多样性及相关性研究[J].植物遗传资源学报,2015,16(3):497-502.GE L W,GUO W,PAN Z K,et al.The morphological diversity and correlation research of Gu Jing wild tea plant from Guizhou[J].Journal of Plant Genetic Resources,2015,16(3):497-502.(in Chinese with English abstract)
[24] 张小琴,周富裕,杨春,等.贵定鸟王种茶树资源农艺性状和品质性状多样性分析[J].分子植物育种,2015,13(2):415-423.ZHANG X Q,ZHOU F Y,YANG C,et al.Diversity of tea germplasm resource (Camellia sinensis ‘Guiding-niaowangzhong’) revealed based on agronomic and quality traits[J].Molecular Plant Breeding,2015,13(2):415-423.(in Chinese with English abstract)
[25] 于晓虎.茄子种质资源形态学标记及ISSR、SSR遗传多样性分析[D].重庆:西南大学,2012.YU X H.Analysis of genetic variation in eggplant using morphological and ISSR,SSR makers[D].Chongqing:Southwest University,2012.(in Chinese with English abstract)
[26] 段志芬,刘本英,汪云刚,等.云南野生茶树资源农艺性状多样性分析[J].西北农业学报,2013,22(1):125-131.DUAN Z F,LIU B Y,WANG Y G,et al.Diversity analysis of agronomic traits of wild tea plants in Yunnan[J].Acta Agriculturae Boreali-Occidentalis Sinica,2013,22(1):125-131.(in Chinese with English abstract)
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