厌氧反硝化产甲烷体系中喹啉与吲哚共基质的降解特性
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摘要
研究了厌氧反硝化产甲烷体系中,典型含氮杂环化合物喹啉、吲哚作为共基质碳源,厌氧生物对二者的降解特性,及群落分析.结果表明:在共基质条件下,喹啉的存在对吲哚的生物降解有抑制作用,且抑制随喹啉浓度的升高而升高;吲哚的存在对喹啉的生物降解有促进作用,但吲哚浓度过高(150mg/L)抑制了喹啉的降解;喹啉、吲哚共基质时,二者的降解都遵循零级反应动力学;通过GC-MS分析,喹啉的主要中间代谢产物分别为2(1H)喹诺酮与8-羟基-2(1H)喹诺酮;吲哚的主要代谢产物为2-吲哚酮与靛红;通过高通量测序对共基质体系的微生物群落进行分析,发现厌氧功能菌群得到富集,细菌菌门以变形菌门Proteobacteria为主,菌纲以Gammaproteobacteria和Betaproteobacteria为主,菌属以Acinetobacter,Candidimonas,Azospira,和Desulfomicrobium为主.
Bath experimrnts were conducted to study the degradation characteristics and microbial community of quinoline and indoles co-substrate under anaerobic denitrification and methanogenesis conditions, which were known as typical N-heterocyclic compounds. The results showed that the presence of quinolone could inhibit the degradation of indole, and the inhibition effect was enhanced with the increase of quinoline concentration; the presence of indole could promote the degradation of quinoline, but the high concentration of indole(150 mg/L) inhibited the degradation of quinolone; the kinetics of quinoline and indole was followed the zero-order kinetics model; through GC-MS analyses, the intermediate metabolites of quinoline were 2-hydroxyquinoline and 2,8-dihydroxyquinoline; and metabolites of indole were oxindole and isatin. The high-throughput sequencing technology was used to analyze the microbial community structure, and results indicated that the functional bacterial were enriched in the anaerobic denitrification and methanogenesis system. The bacterial phylum was Proteobacteria, the dominant classes were Gammaproteobacteria and Betaproteobacteria, and the dominant genera were Acinetobacter, Candidimonas, Azospira, and Desulfomicrobium.
Bath experimrnts were conducted to study the degradation characteristics and microbial community of quinoline and indoles co-substrate under anaerobic denitrification and methanogenesis conditions, which were known as typical N-heterocyclic compounds. The results showed that the presence of quinolone could inhibit the degradation of indole, and the inhibition effect was enhanced with the increase of quinoline concentration; the presence of indole could promote the degradation of quinoline, but the high concentration of indole(150 mg/L) inhibited the degradation of quinolone; the kinetics of quinoline and indole was followed the zero-order kinetics model; through GC-MS analyses, the intermediate metabolites of quinoline were 2-hydroxyquinoline and 2,8-dihydroxyquinoline; and metabolites of indole were oxindole and isatin. The high-throughput sequencing technology was used to analyze the microbial community structure, and results indicated that the functional bacterial were enriched in the anaerobic denitrification and methanogenesis system. The bacterial phylum was Proteobacteria, the dominant classes were Gammaproteobacteria and Betaproteobacteria, and the dominant genera were Acinetobacter, Candidimonas, Azospira, and Desulfomicrobium.
引文
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[23]Vaz-moreira I,Figueira V,Lopes A R,et al.Candidimonas nitroreducens gen.nov.,sp.nov.and Candidimonas humi sp.nov.,isolated from sewage sludge compost[J].Int.J.Syst.Evol.Microbiol.,2011,61(9):2238-46.
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[26]Leahy J G,Olsen R H.Kinetics of toluene degradation by toluene‐oxidizing bacteria as a function of oxygen concentration,and the effect of nitrate[J].Fems Microbiology Ecology,1997,23(1):23-30.
[2]Mandal T,Maity S,Dasgupta D,et al.Advanced oxidation process and biotreatment:Their roles in combined industrial wastewater treatment[J].Desalination,2010,250(1):87-94.
[3]Schwarz G,Senghass E,Erben A,et al.Microbial metabolism of quinoline and related compounds:I.Isolation and characterization of quinoline-degrading bacteria[J].Systematic and Applied Microbiology,1988,10(2):185-90.
[4]Mosquera-corral A,Sanchez M,Campos J L,et al.Simultaneous methanogenesis and denitrification of pretreated effluents from a fish canning industry[J].Water Research,2001,35(2):411-8.
[5]周洪政,刘平,张静,等.微气泡臭氧催化氧化-生化耦合处理难降解含氮杂环芳烃[J].中国环境科学,2017,37(8):2978-85.Zhou H Z,Liu P,Zhang J,et al.Removal of refractory nitrogencontaining heterocyclic aromatics by combination treatment of microbubble catalytic ozonation and biological process[J].China Environmrntal Science,2017,37(8):2978-85.
[6]Berry D F,Francis A J,Bollag J M,et al.Microbial metabolism of homocyclic and heterocyclic aromatic compounds under anaerobic conditions[J].Microbiological Reviews,1987,51(1):43.
[7]Berry D F,Madsen E L,Bollag J M.Conversion of indole to oxindole under methanogenic conditions[J].Applied and Environmental Microbiology,1987,53(1):180-2.
[8]Li Y M,Gu G W,Zhao J F.Anoxic degradation of nitrogenous heterocyclic compounds by acclimated activated sludge[J].Process Biochemistry,2001,37(1):81-6.
[9]Li Y,Li W,Gu G.Promotive effect of pyridine on indole degradation by activated sludge under anoxic conditions[J].Frontiers of Environmental Science and Engineering in China,2007,1(4):493-7.
[10]Zhao Q,Han H,Jia S,et al.Adsorption and bioregeneration in the treatment of phenol,indole,and mixture with activated carbon[J].Desalination and Water Treatment,2015,55(7):1-9.
[11]Zhou A,Zhang J,Varrone C,et al.Process assessment associated to microbial community response provides insight on possible mechanism of waste activated sludge digestion under typical chemical pretreatments[J].Energy,2017,137.
[12]全向春,王建龙,韩力平,等.喹啉与葡萄糖共基质条件下生物降解的动力学分析[J].环境科学学报,2001,21(4):416-9.Quan X C,Wang J L,Han L P,et al.Biodegradation kinetics of a mixture containing quinoline and glucose by burkholderia pickettii strain[J].Act Scientiae Circumstantiae,2001,21(4):416-9.
[13]Wang J,Zhang X,Fan J,et al.Indigoids biosynthesis from Indole by two phenol-degrading strains,Pseudomonas sp.PI1 and Acinetobacter sp.PI2[J].Applied Biochemistry and Biotechnology,2015,176(5):1263-76.
[14]Shukla O P.Microbial transformation of quinoline by a Pseudomonas sp.[J].Applied and Environmental Microbiology,1986,51(6):1332.
[15]Madsen E L,Bollag J M.Pathway of indole metabolism by a denitrifying microbial community[J].Archives of Microbiology,1988,151(1):71-6.
[16]Zhang X,Hua X,Yue X.Comparison of bacterial community characteristics between complete and shortcut denitrification systems for quinoline degradation[J].Applied Microbiology and Biotechnology,2016,101(4):1-11.
[17]Juretschko S,Loy A,Lehner A,et al.The microbial community composition of a nitrifying-denitrifying activated sludge from an industrial sewage treatment plant analyzed by the full-cycle r RNAapproach[J].Systematic and Applied Microbiology,2002,25(1):84-99.
[18]Chen K C,Lin Y F.The relationship between denitrifying bacteria and methanogenic bacteria in a mixed culture system of acclimated sludges[J].Water Research,1993,27(12):1749-59.
[19]Keyser M,Witthuhn R C,Lamprecht C,et al.PCR-based DGGEfingerprinting and identification of methanogens detected in three different types of UASB granules[J].Systematic and Applied Microbiology,2006,29(1):77-84.
[20]Moura A,Tacao M,Henriques I,et al.Characterization of bacterial diversity in two aerated lagoons of a wastewater treatment plant using PCR-DGGE analysis[J].Microbiological Research,2009,164(5):560-9.
[21]Lin G H,Chen H P,Shu H Y.Detoxification of indole by an indoleinduced flavoprotein oxygenase from acinetobacter baumannii[J].Plos One,2015,10(9):e0138798.
[22]Su J F,Zheng S C,Huang T L,et al.Simultaneous removal of Mn(II)and nitrate by the manganese-oxidizing bacterium Acinetobacter sp.SZ28 in anaerobic conditions[J].Geomicrobiology,2016,33(7):586-591.
[23]Vaz-moreira I,Figueira V,Lopes A R,et al.Candidimonas nitroreducens gen.nov.,sp.nov.and Candidimonas humi sp.nov.,isolated from sewage sludge compost[J].Int.J.Syst.Evol.Microbiol.,2011,61(9):2238-46.
[24]Nam J-H,Ventura J-R,Tae I,et al.A novel perchlorate-and nitrate-reducing bacterium,Azospira sp.PMJ[J].Applied Microbiology&Biotechnology,2016,100:1-14.
[25]Krumholz L R,Harris S H,Tay S T,et al.Characterization of two subsurface H2-utilizing bacteria,Desulfomicrobium hypogeium sp.nov.and Acetobacterium psammolithicum sp.nov.,and their ecological roles[J].Applied and Environmental Microbiology,1999,65(6):2300.
[26]Leahy J G,Olsen R H.Kinetics of toluene degradation by toluene‐oxidizing bacteria as a function of oxygen concentration,and the effect of nitrate[J].Fems Microbiology Ecology,1997,23(1):23-30.
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