再生水灌水水平对土壤重金属及致病菌分布的影响
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摘要
为了探讨再生水不同灌水水平对土壤重金属、活性微生物和典型环境致病菌分布的影响,采用室内土柱灌水实验,研究再生水、自来水不同灌水水平对土壤重金属Cd、Pb、Cu、Zn和土壤细菌、真菌、大肠菌群、大肠埃希氏菌分布的影响.结果表明:相同灌水水平下,与自来水灌溉处理相比,再生水灌溉处理下土壤重金属含量略有提高,但仍远低于《土壤环境质量标准》[GB15618-1995]限值,因此短期再生水灌溉不会造成土壤重金属污染;再生水不同灌水水平对比分析表明,充分灌溉相比非充分灌溉提高了表层土壤Cd、Pb含量,Cu、Zn含量无明显差异.此外,相同灌水水平下,再生水灌溉相比自来水显著提高了表层土壤细菌总数和大肠菌群、大肠埃希氏菌数量,对土壤真菌总数影响不大;再生水不同灌水水平对比分析表明,充分灌溉相比非充分灌溉显著提高了表层土壤细菌和真菌总数及大肠菌群、大肠埃希氏菌数量.土壤重金属与土壤活性微生物及典型环境致病菌之间的相关性分析表明,土壤Cd、Pb、Zn含量与土壤细菌、真菌总数及大肠菌群、大肠埃希氏菌数量之间呈正相关性,推断较低含量重金属对土壤活性微生物及典型致病菌的生长繁殖存在一定程度上的刺激作用.因此,再生水灌溉促进了土壤活性微生物的繁衍,并且在一定程度上增加了土壤重金属和环境致病菌的污染风险;合理控制再生水灌水水平可以有效阻控土壤重金属和致病菌含量.
This study aimed to discern the effect of different levels of irrigation with reclaimed water on soil heavy metals and thedistribution of soil active microorganisms and typical environmental pathogenic bacteria. The effects of two irrigation water qualities(reclaimed water and tap water) and two irrigation levels(full irrigation and insufficient irrigation) on soil heavy metals and thedistribution of soil active microorganisms and typical environmental pathogenic bacteria were studied through indoor soil columnirrigation. Results indicated that reclaimed water irrigation increased the content of heavy metals in soil, but according to the soilenvironmental quality standard, soil after irrigated by reclaimed water did not exceed the standard. Therefore, reclaimed waterirrigation could not cause soil heavy metals pollution. Compared with insufficient irrigation with reclaimed water, full irrigation withreclaimed water significantly increased the content of Cd and Pb in surface soil, there was no significant difference in the content ofCu and Zn. At the same irrigation level, the number of bacteria, coliform group and Escherichia coli in surface soil significantlyincreased with reclaimed water irrigation, there was no significant difference in the number of soil fungi at two irrigation waterquality. The number of bacteria, fungi, coliform group and Escherichia coli in surface soil under full irrigation with reclaimed waterwas significantly higher than with insufficient irrigation using reclaimed water. There was a positive correlation between the contentof heavy metals(Cd, Pb, Zn) and the quantity of bacteria and fungi, and between the content of heavy metals and the quantity ofcoliform group and Escherichia coli. Reclaimed water therefore promoted the reproduction of active microorganisms in surface soil,and increased the risk of soil heavy metals and environmental pathogens pollution. Reasonable irrigation with reclaimed water cantherefore effectively control the content of heavy metals and the quantity of soil pathogens in soil.
This study aimed to discern the effect of different levels of irrigation with reclaimed water on soil heavy metals and thedistribution of soil active microorganisms and typical environmental pathogenic bacteria. The effects of two irrigation water qualities(reclaimed water and tap water) and two irrigation levels(full irrigation and insufficient irrigation) on soil heavy metals and thedistribution of soil active microorganisms and typical environmental pathogenic bacteria were studied through indoor soil columnirrigation. Results indicated that reclaimed water irrigation increased the content of heavy metals in soil, but according to the soilenvironmental quality standard, soil after irrigated by reclaimed water did not exceed the standard. Therefore, reclaimed waterirrigation could not cause soil heavy metals pollution. Compared with insufficient irrigation with reclaimed water, full irrigation withreclaimed water significantly increased the content of Cd and Pb in surface soil, there was no significant difference in the content ofCu and Zn. At the same irrigation level, the number of bacteria, coliform group and Escherichia coli in surface soil significantlyincreased with reclaimed water irrigation, there was no significant difference in the number of soil fungi at two irrigation waterquality. The number of bacteria, fungi, coliform group and Escherichia coli in surface soil under full irrigation with reclaimed waterwas significantly higher than with insufficient irrigation using reclaimed water. There was a positive correlation between the contentof heavy metals(Cd, Pb, Zn) and the quantity of bacteria and fungi, and between the content of heavy metals and the quantity ofcoliform group and Escherichia coli. Reclaimed water therefore promoted the reproduction of active microorganisms in surface soil,and increased the risk of soil heavy metals and environmental pathogens pollution. Reasonable irrigation with reclaimed water cantherefore effectively control the content of heavy metals and the quantity of soil pathogens in soil.
引文
[1]Wang Z,Li J,Li Y.Using Reclaimed water for agricultural and landscape irrigation in China:a review[J].Irrigation and Drainage,2017,66(5):672-686.
[2]Khamisi S A A,Prathapar S A,Ahmed M.Conjunctive use of reclaimed water and groundwater in crop rotations[J].Agricultural Water Management,2013,116(1):228-234.
[3]Lyu S,Chen W,Zhang W,et al.Wastewater reclamation and reuse in China:Opportunities and challenges[J].Journal of Environmental Sciences,2016,39:86-96.
[4]陈卫平,吕斯丹,张炜铃,等.再生(污)水灌溉生态风险与可持续利用[J].生态学报,2014,34(1):163-172.Chen W P,Lu S D,Zhang W L,et al.Ecological risks and sustainable utilization of reclaimed water and wastewater irrigation[J].Acta Ecologica Sinica,2014,34(1):163-172.
[5]Urbano V R,Mendon?a T G,Bastos R G,et al.Effects of treated wastewater irrigation on soil properties and lettuce yield[J].Agricultural water management,2017,181:108-115.
[6]Troldborg M,Duckett D,Allan R,et al.A risk-based approach for developing standards for irrigation with reclaimed water.[J].Water Research,2017,126:372-384.
[7]Al-Jassim N,Ansari M I,Harb M,et al.Removal of bacterial contaminants and antibiotic resistance genes by conventional wastewater treatment processes in Saudi Arabia:Is the treated wastewater safe to reuse for agricultural irrigation?[J].Water Research,2015,73(1):277-290.
[8]Pedrero F,Maestre-Valero J F,Mounzer O,et al.Physiological and agronomic mandarin trees performance under saline reclaimed water combined with regulated deficit irrigation[J].Agricultural Water Management,2014,146:228-237.
[9]Lu S,Wang J,Pei L.Study on the effects of irrigation with reclaimed water on the content and distribution of heavy metals in soil[J].International journal of environmental research and public health,2016,13(3):298.
[10]Chen W,Lu S,Pan N,et al.Impact of reclaimed water irrigation on soil health in urban green areas[J].Chemosphere,2015,119:654-661.
[11]王志超,史海滨,李仙岳,等.回填土下再生水灌溉对玉米生长及土壤理化性质的影响[J].水土保持学报,2016,30(1):196-202.Wang Z C,Shi H B,Li X Y,et al.Effects of reclaimed water irrigation on the maize growth and physical and chemical properties of soil based on the construction backfill[J].Journal of Soil and Water Conservation,2016,30(1):196-202.
[12]张铁军,宋矗森,陈莉荣,等.再生水灌溉对土壤盐渍化与重金属累积影响研究[J].节水灌溉,2016,(12):72-75.Zhang T J,Song C S,Chen L R,et al.Effect on reclaimed water irrigation on soil salinizationand accumulation of heavy metals[J].Water Saving Irrigation,2016(12):72-75.
[13]丁光晔,樊贵盛,张艳.山西省汾河再生水灌区土壤重金属污染及分布特征[J].灌溉排水学报,2015,34(2):53-55.Ding G Y,Fan G S,Zhang Y.Pollution and Distribution Characteristic of Heavy Metals in Fenhe River Reclaimed Water Irrigation Area of Shanxi Province[J].Journal of Irrigation and Drainage,2015,34(2):53-55.
[14]Chen W,Lu S,Peng C,et al.Accumulation of Cd in agricultural soil under long-term reclaimed water irrigation[J].Environmental Pollution,2013,178(1):294-299.
[15]马闯,杨军,雷梅,等.北京市再生水灌溉对地下水的重金属污染风险[J].地理研究,2012,31(12):2250-2258.Ma C,Yang J,Lei M,et al.Assessing the effect of reclaimed water irrigation on groundwaterpollution of heavy metals in Beijing[J].Geographical Research,2012,31(12):2250-2258.
[16]赵忠明,陈卫平,焦文涛,等.再生水灌溉对土壤性质及重金属垂直分布的影响[J].环境科学,2012,33(12):4094-4099.Zhao Z M,Chen W P,Jiao W T,et al.Effect of reclaimed water irrigation on soil properties and vertical distribution of heavy metal[J].Environmental Science,2012,33(12):4094-4099.
[17]刘源,崔二苹,李中阳,等.生物质炭和果胶对再生水灌溉下玉米生长及养分、重金属迁移的影响[J].水土保持学报,2017,(6):242-248.Liu Y,Cui E P,Li Z Y,et al.Different effects of biochar and pectin on maize growth,nutrient and heavy metals migration irrigated by reclaimed water[J].Journal of Soil and Water Conservation,2017,(6):242-248.
[18]Cirelli G L,Consoli S,Licciardello F,et al.Treated municipal wastewater reuse in vegetable production[J].Agricultural Water Management,2012,104:163-170.
[19]滕宝霞.大肠杆菌与大肠菌群关系的实验研究[J].中国药事,2004,18(3):170-172.Teng B X.An experimental study on the relationship between E.coli and coliform group[J].Chinese Medicine,2004,18(3):170-172.
[20]Vergine P,Saliba R,Salerno C,et al.Fate of the fecal indicator Escherichia coli in irrigation with partially treated wastewater[J].Water research,2015,85:66-73.
[21]Qiu Z,Li J,Zhao W.Effects of lateral depth and irrigation level on nitrate and Escherichia coli leaching in the North China Plain for subsurface drip irrigation applying sewage effluent[J].Irrigation Science,2017,35(6):469-482.
[22]Li J,Wen J.Effects of water managements on transport of E.coli in soil-plant system for drip irrigation applying secondary sewage effluent[J].Agricultural Water Management,2016,178:12-20.
[23]张志华,陈为峰,石岳峰,等.再生水灌溉对苜蓿生长发育和品质的影响[J].应用生态学报,2009,20(11):2659-2664.Zhang Z H,Chen W F,Shi Y F,et al.Effects of irrigation with regenerated water on the growth and quality of alfalfa[J].Chinese Journal of Applied Ecology,2009,20(11):2659-2664.
[24]Meng W,Wang Z,Hu B,et al.Heavy metals in soil and plants after long-term sewage irrigation at Tianjin China:A case study assessment[J].Agricultural Water Management,2016,171:153-161.
[25]郑顺安,郑向群,刘书田,等.再生水灌溉下紫色水稻土颗粒态有机质中重金属的富集特征[J].水土保持学报,2012,26(2):246-250.Zheng S A,Zheng X Q,Liu S T,et al.Heavy metalsenrichmenti inparticulate organic matter from reclaimed water irrigated purplepaddy soil[J].Journal of Soil and Water Conservation,2012,26(2):246-250.
[26]沈仁芳,赵学强.土壤微生物在植物获得养分中的作用[J].生态学报,2015,35(20):6584-6591.Shen R F,Zhao X Q.Role ofsoilmicrobesin theacquisition ofnutrientsby Plants[J].Acta Ecologica Sinica,2015,35(20):6584-6591.
[27]刘远,张辉,熊明华,等.气候变化对土壤微生物多样性及其功能的影响[J].中国环境科学,2016,36(12):3793-3799.Liu Y,Zhang H,Xiong M H,et al.Effect of climate change on soil microbial diversity and function[J].China Environmental Science,2016,36(12):3793-3799.
[28]刘旻霞,李瑞,张灿,等.兰州市南山季节性土壤微生物特征及影响因素[J].中国环境科学,2018,38(7):2722-2730.Liu M X,Li R,Zhang C,et al.Seasonal characteristics and influencing factors of soil microbial in Nanshan,Lanzhou[J].China Environmental Science,2018,38(7):2722-2730.
[29]赵彤,蒋跃利,闫浩,等.土壤氨化过程中微生物作用研究进展[J].应用与环境生物学报,2014,20(2):315-321.Zhao T,Jiang Y L,Yan H,et al.Research advances on microbial function in soil ammonifyingprocess[J].Chinese Journal of Applied and Environmental Biology,2014,20(2):315-321.
[30]Schadt C W,Rosling A.Comment on“Global diversity and geography of soil fungi”[J].Science,2015,348(6242):1438-1438.
[31]Barbi F,Prudent E,Vallon L,et al.Tree species select diverse soil fungal communities expressing different sets of lignocellulolytic enzymeencoding genes[J].Soil Biology and Biochemistry,2016,100:149-159.
[32]Tedersoo L,Bahram M,P?lme S,et al.Global diversity and geography of soil fungi[J].Science,2014,346(6213):1256688.
[33]龚雪,王继华,关健飞,等.再生水灌溉对土壤化学性质及可培养微生物的影响[J].环境科学,2014,35(9):3572-3579.Gong X,Wang J H,Guan J F,et al.Impact of reclaimed water irrigation on soil chemical properties and culturablemicroorganisms[J].Environmental Science,2014,35(9):3572-3579.
[34]Rendueles O,Amherd M,Velicer G J.Positively frequency-dependent interference competition maintains diversity and pervades a natural population of cooperative microbes[J].Current Biology,2015,25(13):1673-1681.
[35]张娟,王艳春.再生水灌溉对植物根际土壤特性和微生物数量的影响[J].节水灌溉,2009,(3):5-8.Zhang J,Wang Y C.Influenceof Irrigationwith Reclaimed Water on Soil Peculiarityand Microbes Quantityof Plant Rhizosphere[J].Water Saving Irrigation,2009,(3):5-8.
[36]裴亮,孙莉英,张体彬.再生水滴灌对蔬菜根际土壤微生物的影响研究[J].中国农村水利水电,2015,(11):39-42.Pei L,Sun L Y,Zhang T B.Effectofsoilmicroorganism of greenswith reclaimed waterdrip irrigation ofruraldomesticsewage[J].China Rural Water and Hydropower,2015,(11):39-42.
[37]郭魏,齐学斌,李中阳,等.不同施氮水平下再生水灌溉对土壤微环境的影响[J].水土保持学报,2015,29(3):311-315.Guo W,Qi X B,Li Z Y,et al.Impact ofreclaimed wateron soilmicroenvironmentunderdifferentnitrogen levels[J].Journal of Soil and Water Conservation,2015,29(3):311-315.
[38]Paruch A M,M?hlum T.Specific features of Escherichia coli that distinguish it from coliform and thermotolerant coliform bacteria and define it as the most accurate indicator of faecal contamination in the environment[J].Ecological Indicators,2012,23:140-142.
[39]丁程成.欧美水质大肠菌群相关检验标准探究[J].环境科技,2010,(1):67-69.Ding C C.Research on Western relevant inspection standards of coliform group in water body[J].Environmental Science and Technology,2010,(1):67-69.
[40]Ren X,Zeng G,Tang L,et al.Sorption,transport and biodegradationan insight into bioavailability of persistent organic pollutants in soil[J].Science of the Total Environment,2018,610:1154-1163.
[41]Yang F,Zhang W,Li J,et al.The enhancement of atrazine sorption and microbial transformation in biochars amended black soils[J].Chemosphere,2017,189:507-516.
[42]Bech T B,Johnsen K,Dalsgaard A,et al.Transport and distribution of Salmonella entericaserovar Typhimurium in loamy and sandy soil monoliths with applied liquid manure[J].Applied and environmental microbiology,2010,76(3):710-714.
[43]Forslund A,Ensink J H,Markussen B,et al.Escherichia coli contamination and health aspects of soil and tomatoes(Solanumlycopersicum L.)subsurface drip irrigated with on-site treated domestic wastewater[J].Water Research,2012,46(18):5917-5934.
[44]Wen J,Li J,Li Y.Wetting patterns and bacterial distributions in different soils from a surface point source applying effluents with varying Escherichia coli concentrations[J].Journal of integrative agriculture,2016,15(7):1625-1637.
[45]Libutti A,Gatta G,Gagliardi A,et al.Agro-industrial wastewater reuse for irrigation of a vegetable crop succession under Mediterranean conditions[J].Agricultural Water Management,2018,196:1-14.
[46]刁展.外源重金属对不同类型土壤养分及微生物活性的影响[D].杨凌:西北农林科技大学,2016.Diao Z.Effects of exogenous heavy metals on nutrients and microbial activities of different soils[D].Yangling:Northwest Agriculture and Forestry University,2016.
[47]朱红梅,李国华,崔静,等.重金属铅对土壤微生物活性的影响[J].南京农业大学学报,2011,34(6):125-128.Zhu H M,Li G H,Cui J,et al.Effects of lead on microbial activities in vegetable soil[J].Journal of Nanjing Agricultural University,2011,34(6):125-128.
[48]Deng L,Zeng G,Fan C,et al.Response of rhizosphere microbial community structure and diversity to heavy metal co-pollution in arable soil[J].Applied microbiology and biotechnology,2015,99(19):8259-8269.
[49]Xie Y,Fan J,Zhu W,et al.Effect of heavy metals pollution on soil microbial diversity and bermudagrass genetic variation[J].Frontiers in plant science,2016,7:755.
[50]Dixit R,Malaviya D,Pandiyan K,et al.Bioremediation of heavy metals from soil and aquatic environment:an overview of principles and criteria of fundamental processes[J].Sustainability,2015,7(2):2189-2212.
[2]Khamisi S A A,Prathapar S A,Ahmed M.Conjunctive use of reclaimed water and groundwater in crop rotations[J].Agricultural Water Management,2013,116(1):228-234.
[3]Lyu S,Chen W,Zhang W,et al.Wastewater reclamation and reuse in China:Opportunities and challenges[J].Journal of Environmental Sciences,2016,39:86-96.
[4]陈卫平,吕斯丹,张炜铃,等.再生(污)水灌溉生态风险与可持续利用[J].生态学报,2014,34(1):163-172.Chen W P,Lu S D,Zhang W L,et al.Ecological risks and sustainable utilization of reclaimed water and wastewater irrigation[J].Acta Ecologica Sinica,2014,34(1):163-172.
[5]Urbano V R,Mendon?a T G,Bastos R G,et al.Effects of treated wastewater irrigation on soil properties and lettuce yield[J].Agricultural water management,2017,181:108-115.
[6]Troldborg M,Duckett D,Allan R,et al.A risk-based approach for developing standards for irrigation with reclaimed water.[J].Water Research,2017,126:372-384.
[7]Al-Jassim N,Ansari M I,Harb M,et al.Removal of bacterial contaminants and antibiotic resistance genes by conventional wastewater treatment processes in Saudi Arabia:Is the treated wastewater safe to reuse for agricultural irrigation?[J].Water Research,2015,73(1):277-290.
[8]Pedrero F,Maestre-Valero J F,Mounzer O,et al.Physiological and agronomic mandarin trees performance under saline reclaimed water combined with regulated deficit irrigation[J].Agricultural Water Management,2014,146:228-237.
[9]Lu S,Wang J,Pei L.Study on the effects of irrigation with reclaimed water on the content and distribution of heavy metals in soil[J].International journal of environmental research and public health,2016,13(3):298.
[10]Chen W,Lu S,Pan N,et al.Impact of reclaimed water irrigation on soil health in urban green areas[J].Chemosphere,2015,119:654-661.
[11]王志超,史海滨,李仙岳,等.回填土下再生水灌溉对玉米生长及土壤理化性质的影响[J].水土保持学报,2016,30(1):196-202.Wang Z C,Shi H B,Li X Y,et al.Effects of reclaimed water irrigation on the maize growth and physical and chemical properties of soil based on the construction backfill[J].Journal of Soil and Water Conservation,2016,30(1):196-202.
[12]张铁军,宋矗森,陈莉荣,等.再生水灌溉对土壤盐渍化与重金属累积影响研究[J].节水灌溉,2016,(12):72-75.Zhang T J,Song C S,Chen L R,et al.Effect on reclaimed water irrigation on soil salinizationand accumulation of heavy metals[J].Water Saving Irrigation,2016(12):72-75.
[13]丁光晔,樊贵盛,张艳.山西省汾河再生水灌区土壤重金属污染及分布特征[J].灌溉排水学报,2015,34(2):53-55.Ding G Y,Fan G S,Zhang Y.Pollution and Distribution Characteristic of Heavy Metals in Fenhe River Reclaimed Water Irrigation Area of Shanxi Province[J].Journal of Irrigation and Drainage,2015,34(2):53-55.
[14]Chen W,Lu S,Peng C,et al.Accumulation of Cd in agricultural soil under long-term reclaimed water irrigation[J].Environmental Pollution,2013,178(1):294-299.
[15]马闯,杨军,雷梅,等.北京市再生水灌溉对地下水的重金属污染风险[J].地理研究,2012,31(12):2250-2258.Ma C,Yang J,Lei M,et al.Assessing the effect of reclaimed water irrigation on groundwaterpollution of heavy metals in Beijing[J].Geographical Research,2012,31(12):2250-2258.
[16]赵忠明,陈卫平,焦文涛,等.再生水灌溉对土壤性质及重金属垂直分布的影响[J].环境科学,2012,33(12):4094-4099.Zhao Z M,Chen W P,Jiao W T,et al.Effect of reclaimed water irrigation on soil properties and vertical distribution of heavy metal[J].Environmental Science,2012,33(12):4094-4099.
[17]刘源,崔二苹,李中阳,等.生物质炭和果胶对再生水灌溉下玉米生长及养分、重金属迁移的影响[J].水土保持学报,2017,(6):242-248.Liu Y,Cui E P,Li Z Y,et al.Different effects of biochar and pectin on maize growth,nutrient and heavy metals migration irrigated by reclaimed water[J].Journal of Soil and Water Conservation,2017,(6):242-248.
[18]Cirelli G L,Consoli S,Licciardello F,et al.Treated municipal wastewater reuse in vegetable production[J].Agricultural Water Management,2012,104:163-170.
[19]滕宝霞.大肠杆菌与大肠菌群关系的实验研究[J].中国药事,2004,18(3):170-172.Teng B X.An experimental study on the relationship between E.coli and coliform group[J].Chinese Medicine,2004,18(3):170-172.
[20]Vergine P,Saliba R,Salerno C,et al.Fate of the fecal indicator Escherichia coli in irrigation with partially treated wastewater[J].Water research,2015,85:66-73.
[21]Qiu Z,Li J,Zhao W.Effects of lateral depth and irrigation level on nitrate and Escherichia coli leaching in the North China Plain for subsurface drip irrigation applying sewage effluent[J].Irrigation Science,2017,35(6):469-482.
[22]Li J,Wen J.Effects of water managements on transport of E.coli in soil-plant system for drip irrigation applying secondary sewage effluent[J].Agricultural Water Management,2016,178:12-20.
[23]张志华,陈为峰,石岳峰,等.再生水灌溉对苜蓿生长发育和品质的影响[J].应用生态学报,2009,20(11):2659-2664.Zhang Z H,Chen W F,Shi Y F,et al.Effects of irrigation with regenerated water on the growth and quality of alfalfa[J].Chinese Journal of Applied Ecology,2009,20(11):2659-2664.
[24]Meng W,Wang Z,Hu B,et al.Heavy metals in soil and plants after long-term sewage irrigation at Tianjin China:A case study assessment[J].Agricultural Water Management,2016,171:153-161.
[25]郑顺安,郑向群,刘书田,等.再生水灌溉下紫色水稻土颗粒态有机质中重金属的富集特征[J].水土保持学报,2012,26(2):246-250.Zheng S A,Zheng X Q,Liu S T,et al.Heavy metalsenrichmenti inparticulate organic matter from reclaimed water irrigated purplepaddy soil[J].Journal of Soil and Water Conservation,2012,26(2):246-250.
[26]沈仁芳,赵学强.土壤微生物在植物获得养分中的作用[J].生态学报,2015,35(20):6584-6591.Shen R F,Zhao X Q.Role ofsoilmicrobesin theacquisition ofnutrientsby Plants[J].Acta Ecologica Sinica,2015,35(20):6584-6591.
[27]刘远,张辉,熊明华,等.气候变化对土壤微生物多样性及其功能的影响[J].中国环境科学,2016,36(12):3793-3799.Liu Y,Zhang H,Xiong M H,et al.Effect of climate change on soil microbial diversity and function[J].China Environmental Science,2016,36(12):3793-3799.
[28]刘旻霞,李瑞,张灿,等.兰州市南山季节性土壤微生物特征及影响因素[J].中国环境科学,2018,38(7):2722-2730.Liu M X,Li R,Zhang C,et al.Seasonal characteristics and influencing factors of soil microbial in Nanshan,Lanzhou[J].China Environmental Science,2018,38(7):2722-2730.
[29]赵彤,蒋跃利,闫浩,等.土壤氨化过程中微生物作用研究进展[J].应用与环境生物学报,2014,20(2):315-321.Zhao T,Jiang Y L,Yan H,et al.Research advances on microbial function in soil ammonifyingprocess[J].Chinese Journal of Applied and Environmental Biology,2014,20(2):315-321.
[30]Schadt C W,Rosling A.Comment on“Global diversity and geography of soil fungi”[J].Science,2015,348(6242):1438-1438.
[31]Barbi F,Prudent E,Vallon L,et al.Tree species select diverse soil fungal communities expressing different sets of lignocellulolytic enzymeencoding genes[J].Soil Biology and Biochemistry,2016,100:149-159.
[32]Tedersoo L,Bahram M,P?lme S,et al.Global diversity and geography of soil fungi[J].Science,2014,346(6213):1256688.
[33]龚雪,王继华,关健飞,等.再生水灌溉对土壤化学性质及可培养微生物的影响[J].环境科学,2014,35(9):3572-3579.Gong X,Wang J H,Guan J F,et al.Impact of reclaimed water irrigation on soil chemical properties and culturablemicroorganisms[J].Environmental Science,2014,35(9):3572-3579.
[34]Rendueles O,Amherd M,Velicer G J.Positively frequency-dependent interference competition maintains diversity and pervades a natural population of cooperative microbes[J].Current Biology,2015,25(13):1673-1681.
[35]张娟,王艳春.再生水灌溉对植物根际土壤特性和微生物数量的影响[J].节水灌溉,2009,(3):5-8.Zhang J,Wang Y C.Influenceof Irrigationwith Reclaimed Water on Soil Peculiarityand Microbes Quantityof Plant Rhizosphere[J].Water Saving Irrigation,2009,(3):5-8.
[36]裴亮,孙莉英,张体彬.再生水滴灌对蔬菜根际土壤微生物的影响研究[J].中国农村水利水电,2015,(11):39-42.Pei L,Sun L Y,Zhang T B.Effectofsoilmicroorganism of greenswith reclaimed waterdrip irrigation ofruraldomesticsewage[J].China Rural Water and Hydropower,2015,(11):39-42.
[37]郭魏,齐学斌,李中阳,等.不同施氮水平下再生水灌溉对土壤微环境的影响[J].水土保持学报,2015,29(3):311-315.Guo W,Qi X B,Li Z Y,et al.Impact ofreclaimed wateron soilmicroenvironmentunderdifferentnitrogen levels[J].Journal of Soil and Water Conservation,2015,29(3):311-315.
[38]Paruch A M,M?hlum T.Specific features of Escherichia coli that distinguish it from coliform and thermotolerant coliform bacteria and define it as the most accurate indicator of faecal contamination in the environment[J].Ecological Indicators,2012,23:140-142.
[39]丁程成.欧美水质大肠菌群相关检验标准探究[J].环境科技,2010,(1):67-69.Ding C C.Research on Western relevant inspection standards of coliform group in water body[J].Environmental Science and Technology,2010,(1):67-69.
[40]Ren X,Zeng G,Tang L,et al.Sorption,transport and biodegradationan insight into bioavailability of persistent organic pollutants in soil[J].Science of the Total Environment,2018,610:1154-1163.
[41]Yang F,Zhang W,Li J,et al.The enhancement of atrazine sorption and microbial transformation in biochars amended black soils[J].Chemosphere,2017,189:507-516.
[42]Bech T B,Johnsen K,Dalsgaard A,et al.Transport and distribution of Salmonella entericaserovar Typhimurium in loamy and sandy soil monoliths with applied liquid manure[J].Applied and environmental microbiology,2010,76(3):710-714.
[43]Forslund A,Ensink J H,Markussen B,et al.Escherichia coli contamination and health aspects of soil and tomatoes(Solanumlycopersicum L.)subsurface drip irrigated with on-site treated domestic wastewater[J].Water Research,2012,46(18):5917-5934.
[44]Wen J,Li J,Li Y.Wetting patterns and bacterial distributions in different soils from a surface point source applying effluents with varying Escherichia coli concentrations[J].Journal of integrative agriculture,2016,15(7):1625-1637.
[45]Libutti A,Gatta G,Gagliardi A,et al.Agro-industrial wastewater reuse for irrigation of a vegetable crop succession under Mediterranean conditions[J].Agricultural Water Management,2018,196:1-14.
[46]刁展.外源重金属对不同类型土壤养分及微生物活性的影响[D].杨凌:西北农林科技大学,2016.Diao Z.Effects of exogenous heavy metals on nutrients and microbial activities of different soils[D].Yangling:Northwest Agriculture and Forestry University,2016.
[47]朱红梅,李国华,崔静,等.重金属铅对土壤微生物活性的影响[J].南京农业大学学报,2011,34(6):125-128.Zhu H M,Li G H,Cui J,et al.Effects of lead on microbial activities in vegetable soil[J].Journal of Nanjing Agricultural University,2011,34(6):125-128.
[48]Deng L,Zeng G,Fan C,et al.Response of rhizosphere microbial community structure and diversity to heavy metal co-pollution in arable soil[J].Applied microbiology and biotechnology,2015,99(19):8259-8269.
[49]Xie Y,Fan J,Zhu W,et al.Effect of heavy metals pollution on soil microbial diversity and bermudagrass genetic variation[J].Frontiers in plant science,2016,7:755.
[50]Dixit R,Malaviya D,Pandiyan K,et al.Bioremediation of heavy metals from soil and aquatic environment:an overview of principles and criteria of fundamental processes[J].Sustainability,2015,7(2):2189-2212.
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