孕哺期补硒对仔鼠海马硒蛋白mRNA表达的影响
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摘要
目的探讨孕期和哺乳期补硒对仔鼠海马硒蛋白mRNA表达的影响。方法从孕1 d开始给予母鼠含亚硒酸钠的饮水补硒,低、中、高3个剂量组饮水中亚硒酸钠的浓度分别为400、1600、6400μg/L,自由饮用。补硒持续至仔鼠出生后21d。采用RT-PCR测定仔鼠海马中25种硒蛋白mRNA的表达。结果仔鼠海马中25种硒蛋白mRNA均有不同程度的表达,其中SelW、SelP、GPX4、SelK15、SelK、SelM、SelT和GPX1的表达量明显优于其他硒蛋白,Dio1表达量最低;母鼠孕哺期补硒后,有15个硒蛋白基因表达量发生了不同程度的变化,高剂量组改变尤为显著; Sel15、SPS2、SelW和Txnrd2的表达相对稳定,不受饮食补硒的影响。结论孕哺期补硒能够对仔鼠海马硒蛋白的mRNA表达产生影响,进而影响仔鼠神经发育。
Objective To investigate the effect of selenium supplementation during pregnancy and lactation on the expression of selenoprotein mRNA in hippocampus of progeny mouse. Methods From the first day of pregnancy, pregnancy rats were given water containing sodium selenite for free drinking. The concentration of sodium selenite in drinking water of low, medium and high dose groups were 400, 1600, 6400 μg/L, respectively.Selenium supplementation lasted 21 days after birth of offspring. The expression of selenoprotein mRNA in hippocampus of offspring was determined by RT-PCR. Results Total of 25 kinds of selenoprotein mRNA could be detected in the hippocampus of progeny mouse. The expression levels of SelW, SelP, GPX4, SelK15, SelK, SelM,SelT and GPX1 were significantly higher than other selenoproteins, and which of Dio1 was the lowest. After selenium supplementation during pregnancy and lactation, the expression of 15 selenoprotein genes changed to different degrees, especially in the high-dose group. The expression levels of Sel15, SPS2, SelW and Txnrd2 were relatively stable and not were affected by dietary supplementation. Conclusion Selenium supplementation during pregnancy and lactation can affect the expression of selenoprotein mRNA in hippocampus of progeny mouse, and then influence its neurodevelopment.
Objective To investigate the effect of selenium supplementation during pregnancy and lactation on the expression of selenoprotein mRNA in hippocampus of progeny mouse. Methods From the first day of pregnancy, pregnancy rats were given water containing sodium selenite for free drinking. The concentration of sodium selenite in drinking water of low, medium and high dose groups were 400, 1600, 6400 μg/L, respectively.Selenium supplementation lasted 21 days after birth of offspring. The expression of selenoprotein mRNA in hippocampus of offspring was determined by RT-PCR. Results Total of 25 kinds of selenoprotein mRNA could be detected in the hippocampus of progeny mouse. The expression levels of SelW, SelP, GPX4, SelK15, SelK, SelM,SelT and GPX1 were significantly higher than other selenoproteins, and which of Dio1 was the lowest. After selenium supplementation during pregnancy and lactation, the expression of 15 selenoprotein genes changed to different degrees, especially in the high-dose group. The expression levels of Sel15, SPS2, SelW and Txnrd2 were relatively stable and not were affected by dietary supplementation. Conclusion Selenium supplementation during pregnancy and lactation can affect the expression of selenoprotein mRNA in hippocampus of progeny mouse, and then influence its neurodevelopment.
引文
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[12]潘利斌,范辉政,蒋建东,等.微量元素硒的体内过程及生物学效应研究进展[J].药学学报,2017,52(12):1849-1858.Pang LB,Fan HZ,Jiang JD,et al.Advance in the in vivo metabolism and the biological effects of selenium[J].Acta Pharm Sin,2017,52(12):1849-1858.
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[14]Steinbrenner H,Sies H.Selenium homeostasis and antioxidant selenoproteins in brain:Implications for disorders in the central nervous system[J].Arch Biochem Biophys,2013,536:152-157.
[15]Anouar Y,Lihrmann I,Falluel-Morel A.Selenoprotein T is a key player in ER proteostasis,endocrine homeostasis and neuroprotection[J].Free Radical Bio Med,2018,127:145-152.
[2]杨玉洁,李楠.硒蛋白与神经退行性疾病[J].生物技术进展,2017,7(5):511-517.Yang YJ,Li N.Selenoproteins and neurodegenerative diseases[J].Current Biotechol,2017,7(5):511-517.
[3]刘哲,张峰,梁建斌,等.硒蛋白家族及其功能的研究进展[J].中国兽医杂志,2014,50(7):61-63.Liu Z,Zhang F,Liang JB,et al.Research progress on selenoprotein family and its function[J].Chin J Veter Med,2014,50(7):61-63.
[4]Solovyev ND.Importance of selenium and selenoprotein for brain function:From antioxidant protection to neuronal signaling[J].J Inorgan Biochem,2015,153:1-12.
[5]杨晓光,张在香,田园,等.大鼠脑中的硒蛋白[J].卫生研究,1999,28(3):146-149.Yang XG,Zhang ZT,Tian Y,et al.Selenoproteins in rat brian[J].J Hyg Res,1999,28(3):146-149.
[6]Zhang X,Ye YL,Zhu H,et al.Selenotranscriptomic analyses identify signature selenoproteins in brain regions in a mouse model of Parkinson’s disease[J].PLoS One,2016,11(9):1-15.
[7]胡滨,陈一资,辜雪冬.亚硒酸钠的急性和蓄积性试验研究[J].现代食品科技,2010,26(10):1043-1046,1080.Hu B,Chen YZ,Gu XD.Study of the acute and accumulative toxicity of sodium selenium[J].Mod Food Sci Technol,2010,26(10):1043-1046,1080.
[8]Murano K,Ogino H,Okuno T,et al.Role of supplementary selenium on the induction of insulin resistance and oxidative stress in NSY mice fed a high fat diet[J].Biol Pharm Bull,2018,41:92-98.
[9]Iqbal J,Zhang K,Jin N,et al.Effect of sodium selenate on hippocampal proteome of 3×Tg-AD mice exploring the antioxidant dogma of selenium against Alzheimer's disease[J].ACS Chem Neurosci,2018,9(7):1637-1651.
[10]Cao Z,Shao B,Xu F,et al.Protective effect of selenium on aflatoxin B1-induced testicular toxicity in mice[J].Biol Trace Elem Res,2017,2:233-238.
[11]莫俊銮,张丽君,周继昌,等.过量硒加重二硝基氯苯诱导的小鼠特应性皮炎样表现[J].中华皮肤科杂志,2018,51(7):495-499.Mo JL,Zhang LJ,Zhou JC,et al.Excessive selenium supplementation aggravates atopic dermatitis-like skin lesions induced by dinitrochlorobenzene in mice[J].Chin J Dermatol,2018,51(7):495-499.
[12]潘利斌,范辉政,蒋建东,等.微量元素硒的体内过程及生物学效应研究进展[J].药学学报,2017,52(12):1849-1858.Pang LB,Fan HZ,Jiang JD,et al.Advance in the in vivo metabolism and the biological effects of selenium[J].Acta Pharm Sin,2017,52(12):1849-1858.
[13]Shetty S,Marsicano JR,Copeland PR.Uptake and utilization of selenium from selenoprotein P[J].Biol Trace Elem Res,2018,181(1):54-61.
[14]Steinbrenner H,Sies H.Selenium homeostasis and antioxidant selenoproteins in brain:Implications for disorders in the central nervous system[J].Arch Biochem Biophys,2013,536:152-157.
[15]Anouar Y,Lihrmann I,Falluel-Morel A.Selenoprotein T is a key player in ER proteostasis,endocrine homeostasis and neuroprotection[J].Free Radical Bio Med,2018,127:145-152.