四种芽孢杆菌生防功能初探
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摘要
以实验室分离保存的西姆芽孢杆菌H1401和地衣芽孢杆菌H2202、解淀粉芽孢杆菌H4112、贝莱斯芽孢杆菌H4309为例,探究生防应用中的差异特性。采用MSA-CAS检测培养基检测产铁载体功能,用平板对峙法检测其对两种致病菌尖孢镰刀菌古巴生理4号小种和立枯丝核菌的抑制力,用PCR扩增技术检测抗病相关基因,用高效液相色谱法检测4株供试菌株发酵产物中目标抗病脂肽类化合物。结果显示,仅H1401菌株具有分泌铁载体功能;均有抑制两种致病菌的能力;均有抗病相关基因等。研究表明,H4309菌株和H1401菌株具有较好的拮抗植物致病菌的功能。菌株H1401和H4309可以作为潜在的工程菌株加以开发利用。
引文
[1]陈中义,张杰,黄大昉.植物病害生防芽孢杆菌抗菌机制与遗传改良研究[J].植物病理学报,2003,33(2):97-103
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[6]崔晓,徐艳霞,刘俊杰,等.芽孢杆菌在农业生产中的应用[J].土壤与作物,2019,8(1):32-42.
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[10]谭志远,傅琴梅,彭桂香,等.青香茅和五节芒内生固氮菌的分离与生理生化鉴定[J].应用与环境生物学报,2013,19(4):643-649.
[11]Cao Y,Zhihui X U,Ling N,et al.Isolation and identification of lipopeptides produced by B.subtilis SQR 9 for suppressing Fusarium wilt of cucumber[J].Scientia Horticulturae,2012,135(1):32-39.
[12]欧阳慧.抗猕猴桃果腐病西姆芽孢杆菌的分离鉴定及抑菌活性的初步研究[D].江西农业大学,2017.
[13]陈倩倩,刘波,刘国红,等.华重楼根际土芽胞杆菌多样性研究[J].热带农业科学,2015,35(12):103-107.
[14]Chen L,Shi H,Heng J,et al.Antimicrobial,plant growth-promoting and genomic properties of the peanut endophyte Bacillus velezensis LDO2[J].Microbiological Research,2019,(218):41-48.
[2]王萍兰,刘志杰,李顺举,等.几种蕨类植物内生真菌抑制病原菌的筛选[J].九江学院学报(自然科学版),2007,22(3):51-53
[3]LEONG J.Siderophores:their biochemistry and possible role inthe biocontrol of plant pathogens[J].Annu Rev Phytopathol,1986,(24):187-209.
[4]万学勤,李万可兰,崔志新,等.抗菌微生物及拮抗性代谢产物的种类和应用[J].中国医药生物技术,2009,4(2):137-139.
[5]Annapurna K,Kumar A,Kumar L V,et al.PGPR-Induced Systemic Resistance(ISR)in Plant Disease Management[M].2013Wei G,Kloepper J W,Tuzun S.Induction of systemic resistance of cucumber to Colletotrichum orbiculare by select strains of plant growth-promoting rhizobacteria.[J].Phytopathology,1992,81(12):1508-1512.
[6]崔晓,徐艳霞,刘俊杰,等.芽孢杆菌在农业生产中的应用[J].土壤与作物,2019,8(1):32-42.
[7]BENIZRI E,BAUDOIN E,GUCKERT A.Root colonization by inoculated plant growth-promoting rhizobacteria[J].Biocontrol Sci Technol,2001,(11):557-574.
[8]BLOEMBERG G V,LUGTENBERG B J.Molecular basis of plant growth promotion and biocontrol by rhizobacteria[J].Curr Opin Plant Biol,2001,(4):343-350.
[9]ChenS,Xiang Z,Ping S,et al.Hig hsensitive detection method for siderophores from Pseudomonas[J].Microbiology,2006,33(3):122-127.
[10]谭志远,傅琴梅,彭桂香,等.青香茅和五节芒内生固氮菌的分离与生理生化鉴定[J].应用与环境生物学报,2013,19(4):643-649.
[11]Cao Y,Zhihui X U,Ling N,et al.Isolation and identification of lipopeptides produced by B.subtilis SQR 9 for suppressing Fusarium wilt of cucumber[J].Scientia Horticulturae,2012,135(1):32-39.
[12]欧阳慧.抗猕猴桃果腐病西姆芽孢杆菌的分离鉴定及抑菌活性的初步研究[D].江西农业大学,2017.
[13]陈倩倩,刘波,刘国红,等.华重楼根际土芽胞杆菌多样性研究[J].热带农业科学,2015,35(12):103-107.
[14]Chen L,Shi H,Heng J,et al.Antimicrobial,plant growth-promoting and genomic properties of the peanut endophyte Bacillus velezensis LDO2[J].Microbiological Research,2019,(218):41-48.