硒对铬胁迫下茄子生理特性及铬吸收的影响
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摘要
为探讨硒对茄子中铬胁迫的缓解作用,采用土培盆栽方式,研究了不同铬胁迫水平下,施加不同浓度的硒对茄子生长、生理特性及铬吸收的影响。结果表明,随着铬浓度增加,单一铬处理植株生长明显受到抑制,铬毒害表现为株高、根长、根、茎、叶干重及叶绿素含量受抑制。随着铬浓度的增加,叶片中的叶绿素和可溶性蛋白呈下降趋势,丙二醛(MDA)含量呈上升趋势,超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)活性呈先上升后下降的趋势。铬硒混合处理结果表明硒有减轻茄子铬毒害的作用,不同浓度的铬胁迫下,施加不同浓度的硒缓解效果不同,10,20,40,80mg/kg的铬处理下,分别施用12,24,48,48mg/kg的硒缓解效果较好,茄子的株高、根长、生物量均有所增加,叶绿素含量、可溶性蛋白含量显著提高,丙二醛含量和植株体内铬的积累降低。
To investigate the alleviation of selenium(Se)on the chromium(Cr)toxicity in eggplant,the effects of different concentration of Se on the growth,physiological characteristics and Cr uptake by eggplant under different Cr stress levels were studied by pot experiments.The results showed that with the treatment of single Cr,the plant growth was significantly inhibited with the increase of Cr concentration.The Cr toxicity showed that the plant height,root length,shoot and dry weight and chlorophyll content were inhibited.With the increase of Cr concentrations,the chlorophyll and soluble protein in leaves showed a downward trend,and the content of malondialdehyde(MDA)increased,the activities of superoxide dismutase(SOD),peroxidase(POD)and catalase(CAT)increased first and then decreased.The results of Cr-Se mixed treatment showed that Se could alleviate the Cr toxicity of eggplant.Under different concentrations of Cr stress,Se applied with different concentrations showed different alleviation effects.Studies showed that under the treatment of 10,20,40 and 80 mg/kg Cr,the good alleviation effects could be obtained when applied with 12,24,48 and 48 mg/kg Se respectively.The plant height,root length and biomass of eggplant increased,chlorophyll and soluble protein contents increased significantly,MDA and Cr accumulation in plant decreased.
To investigate the alleviation of selenium(Se)on the chromium(Cr)toxicity in eggplant,the effects of different concentration of Se on the growth,physiological characteristics and Cr uptake by eggplant under different Cr stress levels were studied by pot experiments.The results showed that with the treatment of single Cr,the plant growth was significantly inhibited with the increase of Cr concentration.The Cr toxicity showed that the plant height,root length,shoot and dry weight and chlorophyll content were inhibited.With the increase of Cr concentrations,the chlorophyll and soluble protein in leaves showed a downward trend,and the content of malondialdehyde(MDA)increased,the activities of superoxide dismutase(SOD),peroxidase(POD)and catalase(CAT)increased first and then decreased.The results of Cr-Se mixed treatment showed that Se could alleviate the Cr toxicity of eggplant.Under different concentrations of Cr stress,Se applied with different concentrations showed different alleviation effects.Studies showed that under the treatment of 10,20,40 and 80 mg/kg Cr,the good alleviation effects could be obtained when applied with 12,24,48 and 48 mg/kg Se respectively.The plant height,root length and biomass of eggplant increased,chlorophyll and soluble protein contents increased significantly,MDA and Cr accumulation in plant decreased.
引文
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[13]Ismael M A,Elyamine A M,Zhao Y Y,et al.Can selenium and molybdenum restrain cadmium toxicity to pollen grains in Brassica napus?[J].International Journal of Molecular Sciences,2018,19(8):2163-2188.
[14]Hasanuzzaman M,Anwar H M,Fujita M.Selenium in higher plants:Physiological role,antioxidant metabolism and abiotic stress tolerance[J].Journal of Plant Sciences,2010,5(4):354-375.
[15]袁思莉,余垚,万亚男,等.硒缓解植物重金属胁迫和累积的机制[J].农业资源与环境学报,2014,31(6):545-550.
[16]彭玲,贾芬,田小平,等.硒对油菜根尖镉胁迫的缓解作用[J].环境科学学报,2015,35(8):2597-2604.
[17]赵秀峰,程滨,霍晓兰,等.硒对铅、砷单一污染下小白菜种子萌发和幼苗生长的影响[J].中国农学通报,2017,33(23):51-58.
[18]Shanker K,Mishra S,Srivastava S,et al.Study of mercury-selenium(Hg-Se)interactions and their impact on Hg uptake by the radish(Raphanussativus)plant[J].Food and Chemical Toxicology,1996,34(9):883-886.
[19]张驰,吴永尧,彭振坤,等.硒对油菜苗期脯氨酸和丙二醛含量的影响[J].湖北农业科学,2003,16(2):45-47.
[20]Isamu A,Anwar H,Macus T K.Elevated GSH level increases cadmium resistance through down regulation of Sp1-dependent expression of the cadmium transporter ZIP8[J].Molecular pharmacology,2008,74(3):823-833.
[21]廖琳,胡晓荣,李晖,等.生态环境中镉对生物体毒性作用机理及硒对该毒性拮抗作用的研究进展[J].四川环境,2002,21(2):21-24.
[22]Wu Z L,Yin X B,Banuelos G S,et al.Indications of selenium protection against cadmium and lead toxicity in oilseed rape(Brassica napus L.)[J].Frontiers in Plant Science,2016,15(7):1875-1885.
[23]Qing X J,Zhao X H,Hu C X,et al.Selenium alleviates chromium toxicity by preventing oxidative stress in cabbage(Brassica campestris L.ssp.Pekinensis)leaves[J].Ecotoxicology and Environmental Safety,2015,114:179-189.
[24]张志良.植物生理学实验指导[M].2版.北京:高等教育出版社,1990:297-320.
[25]赵世杰,许长成,邹琦,等.植物组织中丙二醛测定方法的改进[J].植物生理学通讯,1994,30(3):207-210.
[26]王丽萍,张健,胡红玲,等.3种土壤下巨菌草对镉胁迫的生理响应[J].农业环境科学学报,2015,34(12):2252-2260.
[27]Muthu T.Selenium,putrescine,and cadmium influence health-promoting phytochemicals and molecularlevel effects on turnip(Brassica rapassp.rapa)[J].Food Chemistry,2015,173:185-193.
[28]Feng R W,Wei C Y,Tu S X,et al.Detoxification of antimony by selenium and their interaction in paddy rice under hydroponic conditions[J].Microchemical Journal,2011,97(1):57-61.
[29]赵秀峰,张强,程滨,等.硒对砷胁迫下小白菜生理特性及砷吸收的影响[J].环境科学学报,2017,37(9):3583-3589.
[30]谷巍,施国新,杜开,等.汞、镉复合污染对轮叶狐尾藻的毒害影响[J].南京师大学报(自然科学版),2001,24(3):75-79.
[31]陈平,吴秀峰,张伟锋,等.硒对镉胁迫下水稻幼苗叶片元素含量的影响[J].中国生态农业学报,2006,14(3):114-117.
[32]郭锋,樊文华,冯两蕊,等.硒对镉胁迫下菠菜生理特性、元素含量及镉吸收转运的影响[J].环境科学学报,2014,34(2):524-531.
[33]Wang R G,Chen S L,Ma H Y,et al.Genotypic differences in antioxidative stress and salt tolerance of three poplars under salt stress[J].Frontiers of Forestry in China,2006,1(1):82-88.
[34]王爱云,黄姗姗,钟国锋,等.铬胁迫对3种草本植物生长及铬积累的影响[J].环境科学,2012,33(6):2028-2037.
[35]Ma P,Yan B,Zeng Q,et al.Oral exposure of Kunming mice to disononylphthalate induces hepatic and renal tissue injury through the accumulation of ROS.Protective effect of melatonin[J].Food and Chemical Toxicology,2014,68(6):247-256..
[36]Mrittunja S,Lena Q M,Bala R,et al.Effects of selenium on arsenic uptake in arsenic hyper accumulator Pterisvittata L.[J].Bioresource Technology,2009,100(3):1115-1121.
[37]王欣,刘云国,艾比布·努扎艾提,等.苎麻对镉毒害的生理耐性机制及外源精胺的缓解效应[J].农业环境科学学报,2007,26(2):487-493.
[38]于淑慧,周鑫斌,王文华,等.硒对水稻幼苗吸收镉的影响[J].西南大学学报(自然科学版),2013,35(9):17-22.
[2]尹华,王锋,刘文.重金属铬在水环境中的迁移转化规律及其污染防治措施[J].农业与技术,2010,30(5):47-49.
[3]杨亚丽,李友丽,陈青云,等.土壤铅、镉、铬对蔬菜发育影响及迁移规律的研究进展[J].华北农学报,2015,30(增刊1):511-517.
[4]Rayman M P.The importance of selenium to human health[J].Lancet,2000,356(9225):233-241.
[5]Jinhyeong L,Jae-Hyun K,Keunsu C,et al.Investigation of the mechanism of chromium removal in(3-aminopropyl)trimethoxysilane functionalized mesoporous silica[J].Scientific Reports,2018,8(1):12078-12089.
[6]Hugo V L.Contribution of local analysis techniques for the characterization of iron and alloying elements in nitrides:Consequences on the precipitation process in FeSi and Fe-Cr nitridealloys[J].Materials,2018,11(8):1409-1428.
[7]张子栋.六价铬毒性作用及其影响因素[J].生物技术世界,2013(8):71.
[8]谢文强.六价铬对人体急性与慢性危害探究[J].资源节约与环保,2016(7):131.
[9]戴宇,杨重法,郑袁明.土壤-植物系统中铬的环境行为及其毒性评价[J].环境科学,2009,30(11):3432-3440.
[10]Stambulska U Y,Bayliak M M,Lushchak V I.Chromium(VI)toxicity in legume plants:Modulation effects of rhizobial symbiosis[J].BioMed Research International,2018(3):1-13.
[11]Monu A,Bala K,Shweta R,et al.Heavy metal accumulation in vegetables irrigated with water from different sources[J].Food Chemistry,2008,111(4):811-815.
[12]王爱云,钟国锋,徐刚标,等.铬胁迫对芥菜型油菜生理特性和铬富集的影响[J].环境科学,2011,32(6):1717-1725.
[13]Ismael M A,Elyamine A M,Zhao Y Y,et al.Can selenium and molybdenum restrain cadmium toxicity to pollen grains in Brassica napus?[J].International Journal of Molecular Sciences,2018,19(8):2163-2188.
[14]Hasanuzzaman M,Anwar H M,Fujita M.Selenium in higher plants:Physiological role,antioxidant metabolism and abiotic stress tolerance[J].Journal of Plant Sciences,2010,5(4):354-375.
[15]袁思莉,余垚,万亚男,等.硒缓解植物重金属胁迫和累积的机制[J].农业资源与环境学报,2014,31(6):545-550.
[16]彭玲,贾芬,田小平,等.硒对油菜根尖镉胁迫的缓解作用[J].环境科学学报,2015,35(8):2597-2604.
[17]赵秀峰,程滨,霍晓兰,等.硒对铅、砷单一污染下小白菜种子萌发和幼苗生长的影响[J].中国农学通报,2017,33(23):51-58.
[18]Shanker K,Mishra S,Srivastava S,et al.Study of mercury-selenium(Hg-Se)interactions and their impact on Hg uptake by the radish(Raphanussativus)plant[J].Food and Chemical Toxicology,1996,34(9):883-886.
[19]张驰,吴永尧,彭振坤,等.硒对油菜苗期脯氨酸和丙二醛含量的影响[J].湖北农业科学,2003,16(2):45-47.
[20]Isamu A,Anwar H,Macus T K.Elevated GSH level increases cadmium resistance through down regulation of Sp1-dependent expression of the cadmium transporter ZIP8[J].Molecular pharmacology,2008,74(3):823-833.
[21]廖琳,胡晓荣,李晖,等.生态环境中镉对生物体毒性作用机理及硒对该毒性拮抗作用的研究进展[J].四川环境,2002,21(2):21-24.
[22]Wu Z L,Yin X B,Banuelos G S,et al.Indications of selenium protection against cadmium and lead toxicity in oilseed rape(Brassica napus L.)[J].Frontiers in Plant Science,2016,15(7):1875-1885.
[23]Qing X J,Zhao X H,Hu C X,et al.Selenium alleviates chromium toxicity by preventing oxidative stress in cabbage(Brassica campestris L.ssp.Pekinensis)leaves[J].Ecotoxicology and Environmental Safety,2015,114:179-189.
[24]张志良.植物生理学实验指导[M].2版.北京:高等教育出版社,1990:297-320.
[25]赵世杰,许长成,邹琦,等.植物组织中丙二醛测定方法的改进[J].植物生理学通讯,1994,30(3):207-210.
[26]王丽萍,张健,胡红玲,等.3种土壤下巨菌草对镉胁迫的生理响应[J].农业环境科学学报,2015,34(12):2252-2260.
[27]Muthu T.Selenium,putrescine,and cadmium influence health-promoting phytochemicals and molecularlevel effects on turnip(Brassica rapassp.rapa)[J].Food Chemistry,2015,173:185-193.
[28]Feng R W,Wei C Y,Tu S X,et al.Detoxification of antimony by selenium and their interaction in paddy rice under hydroponic conditions[J].Microchemical Journal,2011,97(1):57-61.
[29]赵秀峰,张强,程滨,等.硒对砷胁迫下小白菜生理特性及砷吸收的影响[J].环境科学学报,2017,37(9):3583-3589.
[30]谷巍,施国新,杜开,等.汞、镉复合污染对轮叶狐尾藻的毒害影响[J].南京师大学报(自然科学版),2001,24(3):75-79.
[31]陈平,吴秀峰,张伟锋,等.硒对镉胁迫下水稻幼苗叶片元素含量的影响[J].中国生态农业学报,2006,14(3):114-117.
[32]郭锋,樊文华,冯两蕊,等.硒对镉胁迫下菠菜生理特性、元素含量及镉吸收转运的影响[J].环境科学学报,2014,34(2):524-531.
[33]Wang R G,Chen S L,Ma H Y,et al.Genotypic differences in antioxidative stress and salt tolerance of three poplars under salt stress[J].Frontiers of Forestry in China,2006,1(1):82-88.
[34]王爱云,黄姗姗,钟国锋,等.铬胁迫对3种草本植物生长及铬积累的影响[J].环境科学,2012,33(6):2028-2037.
[35]Ma P,Yan B,Zeng Q,et al.Oral exposure of Kunming mice to disononylphthalate induces hepatic and renal tissue injury through the accumulation of ROS.Protective effect of melatonin[J].Food and Chemical Toxicology,2014,68(6):247-256..
[36]Mrittunja S,Lena Q M,Bala R,et al.Effects of selenium on arsenic uptake in arsenic hyper accumulator Pterisvittata L.[J].Bioresource Technology,2009,100(3):1115-1121.
[37]王欣,刘云国,艾比布·努扎艾提,等.苎麻对镉毒害的生理耐性机制及外源精胺的缓解效应[J].农业环境科学学报,2007,26(2):487-493.
[38]于淑慧,周鑫斌,王文华,等.硒对水稻幼苗吸收镉的影响[J].西南大学学报(自然科学版),2013,35(9):17-22.
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