植物乳杆菌KF5胞外多糖合成条件的优化研究
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摘要
从我国西藏传统乳制品发酵剂藏灵菇(Kefir粒)中分离得到一株具有产胞外多糖(exopolysaccharises,EPS)能力的植物乳杆菌菌株KF5,测得其EPS初始产量为49.32 mg/L。通过单因素及正交试验,优化其EPS的最佳合成条件为:培养时间30 h,培养基初始p H 6.3,接种量3%。培养基初始pH值和接种量对EPS的合成影响不显著,培养时间影响显著(p<0.05)。在最优组合条件下,KF5胞外多糖合成量达到95.58 mg/L,相比于正交优化前提高了26.48%。
The Lactobacillus plantarum KF5 was isolated from Kefir grains(Tibet,China),which could secret exopolysaccharises(EPS)during the growing process.The initial yield of EPS was 49.32 mg/L with phenolsulfuric acid method.The conditions for the synthesis of EPS were optimized with single factor and orthogonal experiments.The optimum conditions were as follows:the incubation time 30 h,the initial pH of medium 6.3 and the inoculum volume 3 %.Moreover,initial pH of culture medium and inoculum volume had no significant effect on the yield of EPS,but the culture time had significantly influence on the production(p<0.05).The yield of L.plantarum KF5 could reach 95.58 mg/L under the optimum conditions,which was higher more than 26.48 %before orthogonal optimization.
The Lactobacillus plantarum KF5 was isolated from Kefir grains(Tibet,China),which could secret exopolysaccharises(EPS)during the growing process.The initial yield of EPS was 49.32 mg/L with phenolsulfuric acid method.The conditions for the synthesis of EPS were optimized with single factor and orthogonal experiments.The optimum conditions were as follows:the incubation time 30 h,the initial pH of medium 6.3 and the inoculum volume 3 %.Moreover,initial pH of culture medium and inoculum volume had no significant effect on the yield of EPS,but the culture time had significantly influence on the production(p<0.05).The yield of L.plantarum KF5 could reach 95.58 mg/L under the optimum conditions,which was higher more than 26.48 %before orthogonal optimization.
引文
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[22]刘翠平,吴正钧,王荫榆,等.响应面法优化干酪乳杆菌LC2W合成胞外多糖的培养条件[J].食品与发酵工业,2008,34(12):85-89
[23]刘燕.高产胞外多糖乳酸菌的筛选及培养条件优化的研究[D].郑州:河南农业大学,2009:14
[24]廖乾伟.侗族酸肉中3株乳酸菌产胞外多糖的研究[D].贵阳:贵州大学,2017:8-11
[2]Surayot U,Wang J G,Seesuriyachan P,et al.Exopolysaccharides from lactic acid bacteria:Structural analysis,molecular weight effect on immunomodulation[J].International Journal of Biological Macromolecules,2014,68(25):233-240
[3]崔学敏.益生菌胞外多糖分离与生物学活性研究[D].沈阳:辽宁大学,2016:14-15
[4]李彬.产胞外多糖菌株的筛选及胞外多糖性质和结构分析[D].南京:南京理工大学,2016:1-8
[5]马静.乳酸菌产生的胞外多糖及其在乳品中的应用研究[D].保定:河北农业大学,2004:8-14
[6]刘慧,张红星,代娟.藏灵菇中嗜热链球菌高产胞外多糖发酵条件的优化[J].中国酿造,2007,26(6):41-45
[7]Cuesta G,Suarez N,Bessio M I,et al.Quantitative determination of pneumococcal capsular polysaccharide serotype 14 using a modification of phenol-sulfuric acid method[J].Journal of Microbiological Methods,2003,52(1):69-73
[8]张春红.嗜热链球菌STI产胞外多糖条件优化及特性研究[D].长春:吉林大学,2011:2-3
[9]Fan T,Hu J G,Fu L D,et al.Optimization of enzymolysis-ultrasonic assisted extraction of polysaccharides from Momordica charabtia L.by response surface methodology[J].Carbohydrate Polymers,2015,115(22):701-706.
[10]De Vuyst L,Vanderveken F,Van de Ven S,et al.Production by and isolation of exopolysaccharides from Streptococcus thermophilus grown in a milk medium and evidence for their growth-associated biosynthesis[J].Journal of Applied Microbiology,1998,84(4):1059-1068
[11]Tallon R,Bressollier P,Urdaci M C.Isolation and characterization of two exopolysaccharides produced by Lactobacillus plantarum EP56[J].Research in microbiology,2003,154(10):705-712
[12]李盛钰,曾宪鹏,杨贞耐.提高乳酸菌胞外多糖产量的途径[J].食品与生物技术学报,2009,28(3):289-293
[13]冯美琴.植物乳杆菌胞外多糖发酵、结构鉴定及其功能特性研究[D].南京:南京农业大学,2012:61-67
[14]Pessione A,Bianco G L,Mangiapane E,et al.Characterization of potentially probiotic lactic acid bacteria isolated from olives:Evaluation of short chain fatty acids production and analysis of the extracellular proteome[J].Food Research International,2015,67:247-254
[15]Prasanna P H P,Grandison A S,Charalampopoulos D.Effect of dairy-based protein sources and temperature on growth,acidification and exopolysaccharide production of Bifidobacterium strains in skim milk[J].Food Research International,2012,47(1):6-12
[16]陶静,许赛信,孟德俊,等.乳酸菌发酵生产胞外多糖条件优化研究[J].食品工业,2017(1):28-31
[17]胡盼盼,宋微,单毓娟.影响乳酸菌胞外多糖产量的因素[J].食品科技,2014,39(9):31-37
[18]Thirugnanasambandhamk K,Sivakumar V,Maran J P.Modeling and investigation of submerged fermentation process to produce extracellular polysaccharide using Lactobacillus confusus[J].Carbohydrate Polymers,2014,114:43-47
[19]Sutherland I W.Biosynthesis of microbial exopolysaccharides[J].Advances in Microbial Physiology,1982,23(23):79-150
[20]Kojic M,Vujcic M,Banina A,et al.Analysis of exopolysaccharide production by Lactobacillus casei CG11,Isolated from cheese[J].Applied and Environmental Microbiology,1992,58(12):4086-4088
[21]于静,曾小群,王鸿飞,等.高产胞外多糖乳酸菌的筛选及其发酵条件优化[J].中国食品学报,2015,15(2):93-98
[22]刘翠平,吴正钧,王荫榆,等.响应面法优化干酪乳杆菌LC2W合成胞外多糖的培养条件[J].食品与发酵工业,2008,34(12):85-89
[23]刘燕.高产胞外多糖乳酸菌的筛选及培养条件优化的研究[D].郑州:河南农业大学,2009:14
[24]廖乾伟.侗族酸肉中3株乳酸菌产胞外多糖的研究[D].贵阳:贵州大学,2017:8-11