成都市某幼儿园儿童肠道双歧杆菌种群结构及其与饮奶量的关系
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摘要
目的测定学龄前儿童肠道双歧杆菌(Bifidobacterium)种群结构,探讨其与饮奶量的关系。方法以成都市某幼儿园200名2～6岁健康儿童作为研究对象,男女各半,通过家长填写问卷调查收集饮奶量信息。收集其粪便,采用实时荧光定量PCR对双歧杆菌属和8个菌种水平进行定量测定,比较不同饮奶量儿童肠道双歧杆菌种群结构差异。结果受试儿童粪便均检出双歧杆菌,菌量为(9.33±0.54) log_(10 )CFU/g。日均饮奶量≥300 mL组儿童肠道短双歧杆菌(B.breve)检出率高于饮奶量<300 mL组(88.9%vs. 78.4%,χ~2=4.039,P=0.044)。日均饮奶量≥300 mL组儿童短双歧杆菌和婴儿双歧杆菌(B.infantis)的菌量高于饮奶量<300 mL组儿童[(7.35±1.25)log_(10 )CFU/g vs.(6.91±1.32)log_(10 )CFU/g,t=-2.096,P=0.038;(7.36±1.35)log_(10 )CFU/g vs.(6.68±1.45)log_(10 )CFU/g,t=-2.412,P=0.018]。结论成都市200名学龄前儿童肠道双歧杆菌属检出率为100%,优势菌种为链状双歧杆菌(B.catenulatum)、长双歧杆菌(B.longum)和短双歧杆菌。饮奶量与短双歧杆菌和婴儿双歧杆菌的定植有关。
OBJECTIVE To determine the population structure of intestinal Bifidobacteria in preschool children, and evaluate its relationship with milk consumption. METHODS The study was performed among 200 healthy children aged 2 to 6 in a kindergarten, Chengdu. Half of the children were female. The information of their milk consumption was collected through questionnaire finished by their parents. The levels of Bifidobacterium and 8 Bifidobacterium species in the subject feces were analyzed by real-time fluorescence quantitative PCR. The differences between the population structures of intestinal Bifidobacteria in children with different milk consumption were compared. RESULTS The detection rate of Bifidobacteria in the feces of the children was 100%, and the bacterial amount was(9.33±0.54) log_(10 )CFU/g. In comparison with the group whose daily milk consumption was less than 300 mL, the detection rate of B. breve(88.9% vs. 78.4%,χ~2=4.039,P=0.044), the amount of B. breve and B. infantis [(7.35±1.25) log_(10 )CFU/g vs.(6.91±1.32) log_(10 )CFU/g,t=-2.096,P=0.038;(7.36±1.35) log_(10 )CFU/g vs.(6.68±1.45) log_(10 )CFU/g,t=-2.412,P=0.018]were higher in the group whose daily milk consumption was no less than 300 mL. CONCLUSION The detection rate of Bifidobacteria is 100% in the 200 preschool children of Chengdu. B. catenulatum, B. longum and B. breve are believed to be major bifidobacterial species in children intestinal microbiota. The study manifests the association between milk consumption and B. breve or B. infantis engraftment.
OBJECTIVE To determine the population structure of intestinal Bifidobacteria in preschool children, and evaluate its relationship with milk consumption. METHODS The study was performed among 200 healthy children aged 2 to 6 in a kindergarten, Chengdu. Half of the children were female. The information of their milk consumption was collected through questionnaire finished by their parents. The levels of Bifidobacterium and 8 Bifidobacterium species in the subject feces were analyzed by real-time fluorescence quantitative PCR. The differences between the population structures of intestinal Bifidobacteria in children with different milk consumption were compared. RESULTS The detection rate of Bifidobacteria in the feces of the children was 100%, and the bacterial amount was(9.33±0.54) log_(10 )CFU/g. In comparison with the group whose daily milk consumption was less than 300 mL, the detection rate of B. breve(88.9% vs. 78.4%,χ~2=4.039,P=0.044), the amount of B. breve and B. infantis [(7.35±1.25) log_(10 )CFU/g vs.(6.91±1.32) log_(10 )CFU/g,t=-2.096,P=0.038;(7.36±1.35) log_(10 )CFU/g vs.(6.68±1.45) log_(10 )CFU/g,t=-2.412,P=0.018]were higher in the group whose daily milk consumption was no less than 300 mL. CONCLUSION The detection rate of Bifidobacteria is 100% in the 200 preschool children of Chengdu. B. catenulatum, B. longum and B. breve are believed to be major bifidobacterial species in children intestinal microbiota. The study manifests the association between milk consumption and B. breve or B. infantis engraftment.
引文
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[14] 金红芝,范小兵,杭晓敏,等.儿童肠道双歧杆菌和乳杆菌种群结构分析[J].微生物学报,2005,45(4):567-570.
[15] KATO K,ODAMAKI T,MITSUYAMA E,et al.Age-related changes in the composition of gut bifidobacterium species[J].Curr Microbiol,2017,74(8):987-995.
[16] MARTIN R,MAKINO H,CETINYUREK YAVUZ A,et al.Early-life events,including mode of delivery and type of feeding,siblings and gender,shape the developing gut microbiota[J].Plos One,2016,11(6):e0158498.
[2] KITAGAWA K,OMOTO C,ODA T,et al.Oral combination vaccine,comprising bifidobacterium displaying hepatitis C virus nonstructural protein 3 and interferon-α,induces strong cellular immunity specific to nonstructural protein 3 in mice [J].Viral Immunol,2017,30(3):196-203.
[3] ZHAO L,ZHAO Y,LIU R H,et al.The transcription factor DAF-16 is essential for increased longevity in C.elegans exposed to bifidobacterium longumBB68[J].Nature,2017,7(1):7408.DOI:10.1038/s41598-017-07974-3.
[4] SAAD M J A,SANTOS A,PRADA P O.Linking gut microbiota and inflammation to obesity and insulin resistance[J].Physiology,2016,31(4):283-293.
[5] LIU J L,AN N,MA C,et al.Correlation analysis of intestinal flora with hypertension[J].Exp Therap Med,2018,16(3):2325-2330.DOI:10.3892/etm.2018.6500.
[6] LIEN D T K,NHUNG B T,KHAN N C,et al.Impact of milk consumption on performance and health of primary school children in rural Vietnam[J].Asia Pacific J Clin Nutr,2009,18(3):326-334.
[7] 杨月欣,王光亚,潘兴昌.中国食物成分表[M].北京:北京大学医学出版社,2009.
[8] 中国营养学会.中国居民膳食指南[M].北京:人民卫生出版社,2016.
[9] BLAUT M,KLAUS S.Intestinal microbiota and obesity[M]//Handbook of Experimental Pharmacology(vol209).Berlin:Springer,2011:251-273.
[10] 杨景,罗绪刚,任发政.长双歧杆菌BBMN68诱导的树突状细胞对小鼠牛乳β-乳球蛋白过敏的缓解作用[J].现代食品科技,2016(7):6-11.
[11] KONDO S,XIAO J Z,SATOH T,et al.Antiobesity effects of Bifidobacterium breve strain B-3 supplementation in a mouse model with high-fat diet-induced obesity[J].Biosci Biotech Bioch,2010,74(8):1656-1661.
[12] LI Z J.Inhibitory effect of Bifidobacterium infantis-mediated sKDR prokaryotic expression system on angiogenesis and growth of Lewis lung cancer in mice[J].BMC Cancer,2012,12(1):155.
[13] 王舒悦,李鸣,郭悦,等.成都地区中老年人肠道双歧杆菌构成初探[J].营养学报,2016,38(3):240-245.
[14] 金红芝,范小兵,杭晓敏,等.儿童肠道双歧杆菌和乳杆菌种群结构分析[J].微生物学报,2005,45(4):567-570.
[15] KATO K,ODAMAKI T,MITSUYAMA E,et al.Age-related changes in the composition of gut bifidobacterium species[J].Curr Microbiol,2017,74(8):987-995.
[16] MARTIN R,MAKINO H,CETINYUREK YAVUZ A,et al.Early-life events,including mode of delivery and type of feeding,siblings and gender,shape the developing gut microbiota[J].Plos One,2016,11(6):e0158498.