基质含量对人工快渗滤池厌氧氨氧化脱氮的影响
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摘要
采用人工快渗滤池处理低基质含量污水,考察了进水NO_2~--N、NH_4~+-N含量对厌氧氨氧化(ANAMMOX)脱氮性能的影响。结果表明,人工快渗滤池在进水ρ(NO_2~--N)/ρ(NH_4~+-N)为1.3时ANAMMOX脱氮效果最佳,NH_4~+-N、NO_2~--N、TN平均去除率分别高于98%、98%、91%。对ANAMMOX活性开始产生抑制作用的NH_4~+-N、NO_2~--N质量浓度分别约为65、40 mg/L。提高进水NH_4~+-N、NO_2~--N的质量浓度分别至100、50 mg/L时,ANAMMOX性能受到严重抑制,TN平均去除率分别降至62.2%、45.7%。受NO_2~--N严重抑制时,降低进水NO_2~--N的质量浓度至26 mg/L运行21 d后,TN去除率可恢复至受抑前的84.9%;受NH_4~+-N严重抑制时,降低进水NH_4~+-N的质量浓度至20 mg/L运行16 d后,TN去除率可恢复至受抑前的96.3%。NO_2~--N对ANAMMOX的抑制效应比NH_4~+-N更强,所需的恢复时间更长。
The effect of influent NO2--N and NH4+-N content on the nitrogen removal performance of ANAMMOX was investigated by using constructed rapid infiltration filter to treat wastewater with low substrate content. The results showed that the nitrogen removal efficiency of ANAMMOX was the best when the influent ρ(NO2--N)/ρ(NH4+-N) was 1.3, and the average removal rates of NH4+-N, NO2--N and TN were higher than 98%, 98% and 91%respectively, mass concentration of NH4+-N and NO2--N that inhibited the activity of ANAMMOX was respectively about 65 and 40 mg/L. When mass concentration of NH4+-N and NO2--N respectively increased to 100 and 50 mg/L, the ANAMMOX performance of the filter was severely inhibited, and the average TN removal rate decreased to 62.2% and 45.7%, respectively. When the ANAMMOX performance was seriously inhibited by NO2--N, TN removal rate could be recovered to 84.9% of the pre-inhibition level after 21 d via reducing the influent mass concentration of NO2--N to 26 mg/L. When the ANAMMOX performance was seriously inhibited by NH4+-N, TN removal rate could be recovered to 96.3% of the pre-inhibition level after 16 d via reducing the influent mass concentration of NH4+-N to 20 mg/L. The inhibitory effect of NO2--N on ANAMMOX was stronger than that of NH4+-N, and its recovery time was longer.
The effect of influent NO2--N and NH4+-N content on the nitrogen removal performance of ANAMMOX was investigated by using constructed rapid infiltration filter to treat wastewater with low substrate content. The results showed that the nitrogen removal efficiency of ANAMMOX was the best when the influent ρ(NO2--N)/ρ(NH4+-N) was 1.3, and the average removal rates of NH4+-N, NO2--N and TN were higher than 98%, 98% and 91%respectively, mass concentration of NH4+-N and NO2--N that inhibited the activity of ANAMMOX was respectively about 65 and 40 mg/L. When mass concentration of NH4+-N and NO2--N respectively increased to 100 and 50 mg/L, the ANAMMOX performance of the filter was severely inhibited, and the average TN removal rate decreased to 62.2% and 45.7%, respectively. When the ANAMMOX performance was seriously inhibited by NO2--N, TN removal rate could be recovered to 84.9% of the pre-inhibition level after 21 d via reducing the influent mass concentration of NO2--N to 26 mg/L. When the ANAMMOX performance was seriously inhibited by NH4+-N, TN removal rate could be recovered to 96.3% of the pre-inhibition level after 16 d via reducing the influent mass concentration of NH4+-N to 20 mg/L. The inhibitory effect of NO2--N on ANAMMOX was stronger than that of NH4+-N, and its recovery time was longer.
引文
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[3]WANG S,PENG Y,MA B,et al.Anaerobic ammonium oxidation in traditional municipal wastewater treatment plants with low-strength ammonium loading:widespread but overlooked[J].Water Research,2015,84:66-75.
[4]LI X,SUN S,BADGLEY B D,et al.Nitrogen removal by granular nitritation-anammox in an upflow membrane-aerated biofilm reactor[J].Water Research,2016,94:23-31.
[5]STROUS M,HEIJNEN J J,KUENEN J G,et al.The sequencing batch reactor as a powerful tool for the study of slowly growing anaerobic ammonium-oxidizing microorganisms[J].Applied Microbiology&Biotechnology,1998,50(5):589-596.
[6]ZHU W,LI J,DONG H,et al.Effect of influent substrate ratio on anammox granular sludge:performance and kinetics[J].Biodegradation,2017,28(6):1-16.
[7]YU Y C,TAO Y,GAO D W.Effects of HRT and nitrite/ammonia ratio on anammox discovered in a sequencing batch biofilm reactor[J].Rsc Advances,2014,4(97):54798-54804.
[8]魏琛,陆天友,钟仁超,等.HRT及氮素负荷对厌氧氨氧化系统的影响[J].环境科学学报,2010,30(4):749-755.
[9]LOTTI T,WR V D S,KLEEREBEZEM R,et al.The effect of nitrite inhibition on the anammox process[J].Water Research,2012,46(8):2559-2569.
[10]BETTAZZI E,CAFFAZ S,VANNINI C,et al.Nitrite inhibition and intermediates effects on Anammox bacteria:A batch-scale experimental study[J].Process Biochemistry,2010,45(4):573-580.
[11]CHILD M S,PECHUKAS P.Molecular collision theory[J].Physics Today,1976,29(3):59-60.
[12]唐崇俭,郑平.厌氧氨氧化膨胀污泥床反应器的化学计量学特性[J].中国环境科学,2010,30(11):1446-1452.
[13]JIN R C,XING B S,YU J J,et al.The importance of the substrate ratio in the operation of the Anammox process in upflow biofilter[J].Ecological Engineering,2013,53(3):130-137.
[14]严锋,袁林江,王洋,等.亚硝氮停供及恢复供给对Anammox反应器中氮去除的影响[J].环境科学学报,2017,37(12):4602-4609.
[15]STROUS M,KUENEN J G,JETTEN M S M.Key physiology of anaerobic ammonium oxidation[J].Applied&Environmental Microbiology,1999,65(7):3248-3250.
[16]郑平,胡宝兰.厌氧氨氧化菌混培物生长及代谢动力学研究[J].生物工程学报,2001,17(2):193-198.
[17]DAPENA-MORA A,CAMPOS I F L.Evaluation of activity and inhibition effects on Anammox process by batch tests based on the nitrogen gas production[J].Enzyme&Microbial Technology,2007,40(4):859-865.
[18]丁爽,郑平,毕竑,等.基质性毒物对厌氧氨氧化富集培养物的单独和联合抑制效应[J].环境科学学报,2017,37(8):2936-2943.
[19]唐崇俭,郑平,陈小光.厌氧氨氧化工艺的基质抑制及其恢复策略[J].应用基础与工程科学学报,2010,18(4):561-570.
[20]袁砚,周正,林兴,等.氨氮对厌氧氨氧化过程的抑制规律及调控策略[J].中国环境科学,2017,37(9):3309-3314.
[21]NIU Q,HE S,ZHANG Y,et al.Process stability and the recovery control associated with inhibition factors in a UASB-anammox reactor with a long-term operation[J].Bioresource Technology,2016,203:132-141.
[22]ZHANG Y,NIU Q,MA H,et al.Long-term operation performance and variation of substrate tolerance ability in an anammox attached film expanded bed(AAFEB)reactor[J].Bioresource Technology,2016,211:31-40.