西瓜种质资源枯萎病抗性鉴定
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摘要
以西瓜为试材,采用2种鉴定方法研究了18份种质资源的枯萎病抗性情况。结果表明:供试种质中没有西瓜枯萎病免疫的品种。苗期人工接种法鉴定出高抗西瓜枯萎病材料9份,占参试材料50%;抗病材料1份,感病材料4份,高感病材料4份。其中高抗材料中K1和K4死苗率最低为2%,比高抗对照品种Sugarlee死苗率低。用分子鉴定的方法鉴定出抗病材料6份。苗期田间接种鉴定和分子鉴定结果吻合率达到66.67%。
Watermelon was used as test material,Fusarium wilt resistance of 18 watermelon germplasms were identified by two methods.The results showed that immune varieties were not found in tested material.9 cultivars were high resistance,accounted for 50%;1 cultivar was resistance;4 cultivars were susceptibility;4 cultivars were high susceptibility.Dead seedingsrate of K1 and K4 were 2%,less than the sugarlee control cultivars.6 cultivars showed resistance by using molecular identification.The coincident rate of artificial inoculation at seedling stage and molecular identification was 66.67%.
Watermelon was used as test material,Fusarium wilt resistance of 18 watermelon germplasms were identified by two methods.The results showed that immune varieties were not found in tested material.9 cultivars were high resistance,accounted for 50%;1 cultivar was resistance;4 cultivars were susceptibility;4 cultivars were high susceptibility.Dead seedingsrate of K1 and K4 were 2%,less than the sugarlee control cultivars.6 cultivars showed resistance by using molecular identification.The coincident rate of artificial inoculation at seedling stage and molecular identification was 66.67%.
引文
[1] 张华峰,王迎儿,马海荣,等.嫁接砧木对小果型西瓜生长、产量和品质的影响[J].中国瓜菜,2013,26(6):38-40.
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[11] 张显,王鸣.西瓜枯萎病抗性机制的研究[J].西北农业大学学报,1989,17(4):29-35.
[12] 张屹,张海英,郭绍贵,等.西瓜枯萎病菌生理小种1抗性基因连锁标记开发[J].中国农业科学,2013,46(10):2058-2093.
[13] 张屹,刘建雄,梁志怀,等.不同西瓜品种枯萎病抗性鉴定方法比较[J].长江蔬菜,2015(12):50-52.
[14] 李娜,王吉明,尚建立,等.西瓜枯萎病生理小种1抗性QTL精细定位与InDeI标记开发[J].中国农业科学,2017,50(1):131-141.
[2] 张兆辉,牛青,杨晓峰,等.不同砧木嫁接对西瓜生长、果实品质及丰产性的影响[J].中国农学通报,2015,31(7):72-75.
[3] 刘新华,曹春信,刘林,等.不同砧木对嫁接西瓜生长和果实品质的影响[J].浙江农业学报,2015,27(6):966-969.
[4] 应泉盛,何勇,王迎儿,等.不同砧木嫁接对西瓜果实品质的影响机理研究[J].浙江农业学报,2017,29(4):590-596.
[5] 赵利强.不同种类砧木嫁接对西瓜生长发育和果实品质的影响[D].武汉:华中农业大学,2014.
[6] 朱迎春,孙德玺,邓云,等.野生西瓜嫁接对大果型西瓜生长及品质的影响[J].中国瓜菜,2017,30(12):30-32.
[7] 马双武,刘君璞.西瓜种质资源描述规范和数据标准[M].北京:中国农业出版社,2005.
[8] 焦荻,任毅,宫国义.四倍体西瓜抗枯萎病生理小种1分子标记辅助选择技术研究[J].园艺学报,2015,42(6):1112-1120.
[9] KING B C,WAXMAN K D,NENNI N V,et al.Aresenal of plant cell wall degrading enzymes reflects host preference among plant pathogenic fungi[J].Biotechnology for Biofrels,2012(4):4.
[10] BANI M,RISPAL N,EVIDENTE A,et al.Identification of the main toxins isolated from Fusarium oxysporum f.sp.Pisi race 2 and their relation with isolates pathogenicity[J].Journal of Agricultural and Food Chemistry,2014,62(12):2574-2580.
[11] 张显,王鸣.西瓜枯萎病抗性机制的研究[J].西北农业大学学报,1989,17(4):29-35.
[12] 张屹,张海英,郭绍贵,等.西瓜枯萎病菌生理小种1抗性基因连锁标记开发[J].中国农业科学,2013,46(10):2058-2093.
[13] 张屹,刘建雄,梁志怀,等.不同西瓜品种枯萎病抗性鉴定方法比较[J].长江蔬菜,2015(12):50-52.
[14] 李娜,王吉明,尚建立,等.西瓜枯萎病生理小种1抗性QTL精细定位与InDeI标记开发[J].中国农业科学,2017,50(1):131-141.