鄱阳湖洲滩菰浸泡液对铜绿微囊藻生长的影响
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摘要
为了解鄱阳湖洲滩优势植物菰浸泡液对铜绿微囊藻生长的影响,通过控温、控光的无菌培养条件下,分析菰浸泡液中铜绿微囊藻细胞密度和藻细胞的理化指标的变化。实验结果表明,菰浸泡液对铜绿微囊藻的抑制率较高;培养到第7天时铜绿微囊藻的抑制率在81%～95%之间,并随菰浸泡液生物量含量的增加而增加,当菰浸泡液生物量>8 g/L时铜绿微囊藻抑制率无显著差异(p>0.05)。菰浸泡液对藻细胞产生化感作用可能的原因是化感物质使藻细胞内丙二醛(MDA)含量增加,细胞膜破坏,藻细胞直径缩短,这可能是化感物质导致藻细胞死亡的原因;在培养过程中观测到藻细胞胞外多糖含量增加,这将有利于微囊藻群体的形成而抵御化感抑制。可见,鄱阳湖洲滩优势植物菰浸泡液可抑制铜绿微囊藻的生长,防止藻类水华;该研究也可为菰在水环境中控藻提供基础数据。
To examine the impact of Zizanialatifolia soaking liquid, a dominant plant at the bottom land of Poyang Lake, on the growth of M. aeruginosa under the indoor aseptic condition with temperature and intensity control, the growth curves of M. aeruginosa, the inhibition rate, the cell diameter, the changes of cell MDA and EPS were analyzed. The experimental results showed that Zizanialatifolia soaking liquid played an inhibitory role in the growth of M.aeruginosa, and the inhibitory effect was intensified gradually with the increase of Zizanialatifolia concentration. The inhibition rate ranged from 81% to 95%,the highest inhibitory concentration of Zizanialatifolia soaking liquid is achieved at 8 g/L. The allelochemicals secreted by Zizanialatifolia could increase the contents of malondialdehyde(MDA), it also led to lipidperoxidation, the destruction of cell membrane ion channel and the reduction of cell diameter, which will eventually inhibit the growth of M. aeruginosa. The extracellular polysaccharide content of algae was increased by allelopathy, which would be beneficial to the formation colony in M. aeruginosa, and impaired allelopathy. The growth of M. aeruginosa was inhibited and algae bloom might be prevented in the soaking liquid of the Zizanialatifolia in Poyang Lake. The experimental results provided basic data for controlling water bloom.
To examine the impact of Zizanialatifolia soaking liquid, a dominant plant at the bottom land of Poyang Lake, on the growth of M. aeruginosa under the indoor aseptic condition with temperature and intensity control, the growth curves of M. aeruginosa, the inhibition rate, the cell diameter, the changes of cell MDA and EPS were analyzed. The experimental results showed that Zizanialatifolia soaking liquid played an inhibitory role in the growth of M.aeruginosa, and the inhibitory effect was intensified gradually with the increase of Zizanialatifolia concentration. The inhibition rate ranged from 81% to 95%,the highest inhibitory concentration of Zizanialatifolia soaking liquid is achieved at 8 g/L. The allelochemicals secreted by Zizanialatifolia could increase the contents of malondialdehyde(MDA), it also led to lipidperoxidation, the destruction of cell membrane ion channel and the reduction of cell diameter, which will eventually inhibit the growth of M. aeruginosa. The extracellular polysaccharide content of algae was increased by allelopathy, which would be beneficial to the formation colony in M. aeruginosa, and impaired allelopathy. The growth of M. aeruginosa was inhibited and algae bloom might be prevented in the soaking liquid of the Zizanialatifolia in Poyang Lake. The experimental results provided basic data for controlling water bloom.
引文
[1] Schrader K K, Dhammika N P, Rimando C S, et al. Novel derivatives of 9, 10-anthraquinone are selective algicides against the musty-odor cyanobacterium Oscillatoria perornata[J]. Applied and Environmental Microbiology, 2003,69(9):5319-5327.
[2] Preston T, Stewart W D P, Reynolds C S. Bloom-forming cyanobacterium Microcystis aeruginosa over winters on sediment surface[J]. Nature, 1980,288(5789):365-367.
[3] Reynolds C S, Jaworski G H M, Cmiech H A, et al. On the annual cycle of the blue-green alga Microcystis aeruginosa-philosophical transactions of the Royal Society of London, Series B[J]. Biological Sciences, 1981,293(1068):419-477.
[4] Gumbo RJ G, Ross ET, Cloete. Biological control of Microcystis dominated harmful algal blooms[J]. African Journal of Biotechnology, 2008,7(25):4765-4773.
[5] Rice E L. PREFACE-Allelopathy[M]. Second Edition. Allelopathy, 1983.
[6] Park M, Chung I, Ahmad A B, et al. Growth inhibition of unicellular and colonial Microcystis strains(Cyanophyceae)by compounds isolated from rice(Oryza sativa)hulls[J].Aquatic Botany, 2009,90(4):309-314.
[7] Wang H, Liu F, Luo P, et al. Allelopathic effects of Myriophyllum aquaticum on two cyanobacteria of Anabaena flosaquae and Microcystis aeruginosa[J]. Bulletin of Environmental Contamination and Toxicology, 2017,98(4):556-561.
[8] Pakdel FM, Sim L, Beardall J, et al. Allelopathic inhibition of microalgae by the freshwater stonewort, Chara australis,and a submerged angiosperm, Potamogeton crispus[J].Aquatic Botany, 2013,110(10):24-30.
[9] Takeda F, Nakano K, Aikawa Y, et al. Allelopathic potential against Microcystis aeruginosa by emergent Macrophytes on floating beds[J]. Journal of Water&Environment Technology, 2011,9(4):371-380.
[10] Wu X X, Zhang Z Y, Chen D L, et al. Allelopathic effects of Eichhornia crassipes on the growth of Microcystis aeruginosa[J]. Journal of Agricultural Science and Technology, 2012,12:1400-1406.
[11] Park M H, Han M S, Ahn C Y, et al. Growth inhibition of bloom-forming cyanobacterium Microcystis aeruginosa by rice straw extract[J]. Letters in Applied Microbiology, 2006,43(3):307-312.
[12] Hall A B, Blum U, Fites R C. Stress modification of allelopathy of Helianthus annuus L. debris on seed germination[J].American Journal of Botany, 1982,69(5):776-783.
[13] Hong Y, Hu H Y, Sakoda A, et al. Straw preservation effects of Arundo donax L. on its allelopathic activity to toxic and bloom-forming Microcystis aeruginosa[J]. Water Science and Technology, 2011,63(8):1566-1573.
[14]李林,陈峰,赵荣芳.鄱阳湖灰化苔草浸泡液对铜绿微囊藻的化感作用[J].环境科学与技术, 2016,39(2):1-5.Li Lin, Chen Feng, Zhao Rongfang. Allelopathic effect of soaking Carex cinerascens in Poyang Lake on Microcystis aeruginosa[J]. Environmental Science&Technology, 2016,39(2):1-5.
[15]董龙香,胡利静,胡鲲,等.大蒜对铜绿微囊藻的化感抑制作用[J].江西农业大学学报, 2016,38(6):1167-1173.
[16] Zhang S, Zhang B, Dai W, et al. Oxidative damage and antioxidant responses in Microcystis aeruginosa exposed to the allelochemical berberine isolated from golden thread[J].Journal of Plant Physiology, 2011,168(7):639-643.
[17]李锋民,胡洪营,种云霄,等.芦苇化感物质对藻类细胞膜选择透性的影响[J].环境科学, 2007,28(11):2453-2456.Li Fengmin, Hu Hongying, Chong Yunxiao, et al. Effects of allelochemical isolated from Phragmites communis on algal membrane permeability[J]. Environmental Science, 2007,28(11):2453-2456.
[18] Taylor A R, Manison N F H, Fernandez C, et al. Spatial organization of calcium signaling involved in volume control of the Fucus rhizoid[J]. Plant Cell, 1996,8:2015-2031.
[19] Heimlich G, Bortner C D, Cidlowski J A. Apoptosis and cell volume regulation-the importance of ions and ion channels[J]. Advances in Experimental Medicine&Biology, 2004,559:189-203.
[20]李林,陈峰,赵荣芳.淹没对鄱阳湖洲滩灰化苔草化感抑藻的影响[J].草业科学, 2015,32(11):1832-1837.
[21]戴国飞,张伟,彭宁彦,等.枯水期鄱阳湖及其滨湖水体氮磷等污染物分布与藻华风险研究[J].生态环境学报, 2015,24(5):838-844.Dai Guofei, Zhang Wei, Peng Ningyan, et al. Study on distribution of N and P pollutants and risk of cyanobacteria bloom in Poyang Lake and waters around the lake during drought periods[J]. Ecology and Environmental Science,2015,24(5):838-844.
[22]李冰,杨桂山,王晓龙,等.鄱阳湖典型洲滩植物物种多样性季节动态特征[J].土壤, 2016,48(2):298-305.Li Bing, Yang Guishan, Wang Xiaolong, et al. Seasonal dynamic characteristics of species diversity of typical hygrophilous vegetation in Poyang lakeshore wetland[J]. Soil, 2016,48(2):298-305.
[23]胡振鹏,葛刚,刘成林.鄱阳湖湿地植被退化原因分析及其预警[J].长江流域资源与环境, 2015,24(3):381-386.Hu Zhenpeng, Ge Gang, Liu Chenglin. Cause analysis and early warning for wetland vegetation degradation in Poyang Lake[J]. Resource and Environment in the Yangtze Basin,2015,24(3):381-386.
[24] Heath RL, Packer L. Photoperoxidation in isolated chloroplasts:I. kinetics and stoichiometry of fatty acid peroxidation[J]. Archives of Biochemistry and Biophysics, 1968,125(1):189-198.
[25] Herbert D, Phipps P J, Strange R E. Chemical analysis of microbial cells[J]. Methods in Microbiology, 1971,5:209-344.
[26]国家环保总局《水和废水监测分析方法》编委会.水和废水监测分析方法[M].第4版.北京:中国环境科学出版社,2002:38-47.
[27] Zhang T T, Wang L L, He Z X, et al. Growth inhibition and biochemical changes of cyanobacteria induced by emergent macrophyte Thalia dealbata, roots[J]. Biochemical Systematics&Ecology, 2011,39(2):88-94.
[28]洪喻,胡洪营,黄晶晶,等.不同溶剂提取芦竹化感物质对铜绿微囊藻生长的影响[J].环境科学, 2008,29(11):3143-3147.Hong Yu, Hu Hongying, Huang Jingjing, et al. Growth of Microcystis aeruginosa affected by allelochemicals of Arundo donax Linn. extracted with different solvents[J]. Envionmental Science, 2008,29(11):3143-3147.
[29]唐萍,吴国荣,陆长梅,等.凤眼莲根系分泌物对栅藻结构及代谢的影响[J].环境科学学报, 2000,20(3):355-359.
[30] Xu F, Zhu W, Xiao M, et al. Interspecific variation in extracellular polysaccharide content and colony formation of Microcystis spp. cultured under different light intensities and temperatures[J]. Journal of Applied Phycology, 2015,28(3):1-9.
[2] Preston T, Stewart W D P, Reynolds C S. Bloom-forming cyanobacterium Microcystis aeruginosa over winters on sediment surface[J]. Nature, 1980,288(5789):365-367.
[3] Reynolds C S, Jaworski G H M, Cmiech H A, et al. On the annual cycle of the blue-green alga Microcystis aeruginosa-philosophical transactions of the Royal Society of London, Series B[J]. Biological Sciences, 1981,293(1068):419-477.
[4] Gumbo RJ G, Ross ET, Cloete. Biological control of Microcystis dominated harmful algal blooms[J]. African Journal of Biotechnology, 2008,7(25):4765-4773.
[5] Rice E L. PREFACE-Allelopathy[M]. Second Edition. Allelopathy, 1983.
[6] Park M, Chung I, Ahmad A B, et al. Growth inhibition of unicellular and colonial Microcystis strains(Cyanophyceae)by compounds isolated from rice(Oryza sativa)hulls[J].Aquatic Botany, 2009,90(4):309-314.
[7] Wang H, Liu F, Luo P, et al. Allelopathic effects of Myriophyllum aquaticum on two cyanobacteria of Anabaena flosaquae and Microcystis aeruginosa[J]. Bulletin of Environmental Contamination and Toxicology, 2017,98(4):556-561.
[8] Pakdel FM, Sim L, Beardall J, et al. Allelopathic inhibition of microalgae by the freshwater stonewort, Chara australis,and a submerged angiosperm, Potamogeton crispus[J].Aquatic Botany, 2013,110(10):24-30.
[9] Takeda F, Nakano K, Aikawa Y, et al. Allelopathic potential against Microcystis aeruginosa by emergent Macrophytes on floating beds[J]. Journal of Water&Environment Technology, 2011,9(4):371-380.
[10] Wu X X, Zhang Z Y, Chen D L, et al. Allelopathic effects of Eichhornia crassipes on the growth of Microcystis aeruginosa[J]. Journal of Agricultural Science and Technology, 2012,12:1400-1406.
[11] Park M H, Han M S, Ahn C Y, et al. Growth inhibition of bloom-forming cyanobacterium Microcystis aeruginosa by rice straw extract[J]. Letters in Applied Microbiology, 2006,43(3):307-312.
[12] Hall A B, Blum U, Fites R C. Stress modification of allelopathy of Helianthus annuus L. debris on seed germination[J].American Journal of Botany, 1982,69(5):776-783.
[13] Hong Y, Hu H Y, Sakoda A, et al. Straw preservation effects of Arundo donax L. on its allelopathic activity to toxic and bloom-forming Microcystis aeruginosa[J]. Water Science and Technology, 2011,63(8):1566-1573.
[14]李林,陈峰,赵荣芳.鄱阳湖灰化苔草浸泡液对铜绿微囊藻的化感作用[J].环境科学与技术, 2016,39(2):1-5.Li Lin, Chen Feng, Zhao Rongfang. Allelopathic effect of soaking Carex cinerascens in Poyang Lake on Microcystis aeruginosa[J]. Environmental Science&Technology, 2016,39(2):1-5.
[15]董龙香,胡利静,胡鲲,等.大蒜对铜绿微囊藻的化感抑制作用[J].江西农业大学学报, 2016,38(6):1167-1173.
[16] Zhang S, Zhang B, Dai W, et al. Oxidative damage and antioxidant responses in Microcystis aeruginosa exposed to the allelochemical berberine isolated from golden thread[J].Journal of Plant Physiology, 2011,168(7):639-643.
[17]李锋民,胡洪营,种云霄,等.芦苇化感物质对藻类细胞膜选择透性的影响[J].环境科学, 2007,28(11):2453-2456.Li Fengmin, Hu Hongying, Chong Yunxiao, et al. Effects of allelochemical isolated from Phragmites communis on algal membrane permeability[J]. Environmental Science, 2007,28(11):2453-2456.
[18] Taylor A R, Manison N F H, Fernandez C, et al. Spatial organization of calcium signaling involved in volume control of the Fucus rhizoid[J]. Plant Cell, 1996,8:2015-2031.
[19] Heimlich G, Bortner C D, Cidlowski J A. Apoptosis and cell volume regulation-the importance of ions and ion channels[J]. Advances in Experimental Medicine&Biology, 2004,559:189-203.
[20]李林,陈峰,赵荣芳.淹没对鄱阳湖洲滩灰化苔草化感抑藻的影响[J].草业科学, 2015,32(11):1832-1837.
[21]戴国飞,张伟,彭宁彦,等.枯水期鄱阳湖及其滨湖水体氮磷等污染物分布与藻华风险研究[J].生态环境学报, 2015,24(5):838-844.Dai Guofei, Zhang Wei, Peng Ningyan, et al. Study on distribution of N and P pollutants and risk of cyanobacteria bloom in Poyang Lake and waters around the lake during drought periods[J]. Ecology and Environmental Science,2015,24(5):838-844.
[22]李冰,杨桂山,王晓龙,等.鄱阳湖典型洲滩植物物种多样性季节动态特征[J].土壤, 2016,48(2):298-305.Li Bing, Yang Guishan, Wang Xiaolong, et al. Seasonal dynamic characteristics of species diversity of typical hygrophilous vegetation in Poyang lakeshore wetland[J]. Soil, 2016,48(2):298-305.
[23]胡振鹏,葛刚,刘成林.鄱阳湖湿地植被退化原因分析及其预警[J].长江流域资源与环境, 2015,24(3):381-386.Hu Zhenpeng, Ge Gang, Liu Chenglin. Cause analysis and early warning for wetland vegetation degradation in Poyang Lake[J]. Resource and Environment in the Yangtze Basin,2015,24(3):381-386.
[24] Heath RL, Packer L. Photoperoxidation in isolated chloroplasts:I. kinetics and stoichiometry of fatty acid peroxidation[J]. Archives of Biochemistry and Biophysics, 1968,125(1):189-198.
[25] Herbert D, Phipps P J, Strange R E. Chemical analysis of microbial cells[J]. Methods in Microbiology, 1971,5:209-344.
[26]国家环保总局《水和废水监测分析方法》编委会.水和废水监测分析方法[M].第4版.北京:中国环境科学出版社,2002:38-47.
[27] Zhang T T, Wang L L, He Z X, et al. Growth inhibition and biochemical changes of cyanobacteria induced by emergent macrophyte Thalia dealbata, roots[J]. Biochemical Systematics&Ecology, 2011,39(2):88-94.
[28]洪喻,胡洪营,黄晶晶,等.不同溶剂提取芦竹化感物质对铜绿微囊藻生长的影响[J].环境科学, 2008,29(11):3143-3147.Hong Yu, Hu Hongying, Huang Jingjing, et al. Growth of Microcystis aeruginosa affected by allelochemicals of Arundo donax Linn. extracted with different solvents[J]. Envionmental Science, 2008,29(11):3143-3147.
[29]唐萍,吴国荣,陆长梅,等.凤眼莲根系分泌物对栅藻结构及代谢的影响[J].环境科学学报, 2000,20(3):355-359.
[30] Xu F, Zhu W, Xiao M, et al. Interspecific variation in extracellular polysaccharide content and colony formation of Microcystis spp. cultured under different light intensities and temperatures[J]. Journal of Applied Phycology, 2015,28(3):1-9.
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