大豆抗病分子标记的研究进展
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摘要
大豆(Glycine max L.)病害是影响大豆产量和品质的重要因素之一,随着大豆全基因组序列的公布,以SNP为代表的新一代分子标记技术使大豆抗病分子标记辅助育种得以快速发展。本文综述了2010年以来研究者们对大豆抗性基因的定位方法、抗病基因的定位、候选基因的筛选及鉴定等所取得的新进展,并结合当前大豆抗病分子标记的现状提出了新的发展方向,为进一步探究相关抗病基因的功能及分子育种提供理论参考。
Soybean(Glycine max L.) disease is one of the most important factors affecting soybean yield and quality. With the publication of the whole genome sequence of soybean, SNP as a new generation of molecular marker has been effectively applied in resistance molecular marker-assisted breeding. This article reviews the progress made by researchers with mapping methods, resistance genes localization, screening and identification of candidate genes for soybean disease resistance since 2010. This review could provide a helpful reference for further functional analysis of resistance-related genes and molecular breeding in soybean.
Soybean(Glycine max L.) disease is one of the most important factors affecting soybean yield and quality. With the publication of the whole genome sequence of soybean, SNP as a new generation of molecular marker has been effectively applied in resistance molecular marker-assisted breeding. This article reviews the progress made by researchers with mapping methods, resistance genes localization, screening and identification of candidate genes for soybean disease resistance since 2010. This review could provide a helpful reference for further functional analysis of resistance-related genes and molecular breeding in soybean.
引文
[1]韩立德,盖钧镒,张文明.大豆营养成分研究现状.种子,2003(5):57-59.
[2]王艳,韩英鹏,李文滨.大豆分子标记研究新进展.大豆科学,2015,34(1):148-162.
[3]Martin T,Nikolai A,Mugford S G,et al. Combining SNP discovery from next-generation sequencing data with bulked segregant analysis(BSA)to fine-map genes in polyploid wheat. BMC Plant Biology,2012,12(1):12-14.
[4]Liu W Y,Kang J H,Jeong H S,et al. Combined use of bulked segregant analysis and microarrays reveals SNP markers pinpointing a major QTL for resistance to Phytophthora capsici in pepper.Theoretical and Applied Genetics,2014,127(11):2503-2513.
[5]Wu J,Wang Q,Xu L,et al. Combining SNP genotyping array with bulked segregant analysis to map a gene controlling adultplant resistance to stripe rust in wheat line 03031-1-5 H62.Phytopathology,2017,108(1):103-113.
[6]Zhang X,Li R,Chen L,et al. Fine-mapping and candidate gene analysis of the Brassica juncea white-flowered mutant Bjpc2 using the whole-genome resequencing. Molecular Genetics and Genomics,2017,293(2):359-370.
[7]Han Y,Zhao F,Gao S,et al. Fine mapping of a male sterility gene ms-3 in a novel cucumber(Cucumis sativus L.)mutant. Theoretical and Applied Genetics,2018,131(3):449-460.
[8]张红,王超楠,黄志银,等.近等基因系构建及其在育种中应用综述.中国园艺文摘,2018,34(6):54-56.
[9]Kobayashi T,Yamamoto K,Suetsugu Y,et al. Genetic mapping of the rice resistance-breaking gene of the brown planthopper Nilaparvata lugens. Proceedings Biological Sciences,2014,281:296-306.
[10]Chung C L,Poland J,Kump K,et al. Targeted discovery of quantitative trait loci for resistance to northern leaf blight and other diseases of maize. Theoretical and Applied Genetics,2011,123(2):307-326.
[11]Warrington C V,Zhu S,Parrott W A,et al. Seed yield of nearisogenic soybean lines with introgressed quantitative trait loci conditioning resistance to corn earworm(lepidoptera:noctuidae)and soybean looper(lepidoptera:noctuidae)from PI 229358. Journal of Economic Entomology,2008,101(4):1471-1477.
[12]Zheng Z,Ma J,Stiller J,et al. Fine mapping of a large-effect QTL conferring Fusarium crown rot resistance on the long arm of chromosome 3B in hexaploid wheat. BMC Genomics,2015,16(1):1-7.
[13]Cho E K,Goodman R M. Strains of soybean mosaic virus:classification based on virulence in resistant soybean cultivars.Phytopathology,1979,69(5):467-470.
[14]Yulho K,Oksun K,Bongchoon L,et al. G7H,a new soybean mosaic virus strain:its virulence and nucleotide sequence of CI gene. Plant Disease,2003,87(11):1372-1375.
[15]Yang Q H,Gai J Y. Identification,inheritance and gene mapping of resistance to a virulent soybean mosaic virus strain SC15 in soybean.Plant Breeding,2011,130(2):128-132.
[16]Wang D,Zhao L,Li K,et al. Marker-assisted pyramiding of soybean resistance genes RSC4,RSC8,and RSC14Q to soybean mosaic virus. Journal of Integrative Agriculture,2017,16(11):2413-2420.
[17]Ren R,Liu S,Karthikeyan A,et al. Fine-mapping and identification of a novel locus Rsc15,underlying soybean resistance to soybean mosaic virus. Theoretical and Applied Genetics,2017,130(11):2395-2410.
[18]Yan H,Wang H,Cheng H,et al. Detection and fine-mapping of SC7resistance genes via linkage and association analysis in soybean.Journal of Integrative Plant Biology,2015,57(8):722-729.
[19]Zhao L,Wang D,Zhang H,et al. Fine mapping of the RSC8 locus and expression analysis of candidate SMV resistance genes in soybean. Plant Breeding,2016,135(6):701-706.
[20]Zheng G J,Yang Y Q,Ying M A,et al. Fine mapping and candidate gene analysis of resistance gene RSC3Q,to soybean mosaic virus,in Qihuang 1. Journal of Integrative Agriculture,2014,13(12):2608-2615.
[21]Wang D,Ma Y,Liu N,et al. Fine mapping and identification of the soybean RSC4 resistance candidate gene to soybean mosaic virus.Plant Breeding,2011,130(6):653-659.
[22]Yang Y,Zheng G,Han L,et al. Genetic analysis and mapping of genes for resistance to multiple strains of soybean mosaic virus in a single resistant soybean accession PI 96983. Theoretical and Applied Genetics,2013,126(7):1783-1791.
[23]Ma Y,Wang D,Li H,et al. Fine mapping of the RSC14Q,locus for resistance to soybean mosaic virus in soybean. Euphytica,2011,181(1):127-135.
[24]Li K,Ren R,Adhimoolam K,et al. Genetic analysis and identification of two soybean mosaic virus resistance genes in soybean[Glycine max(L.)Merr]. Plant Breeding,2016,134(6):684-695.
[25]Karthikeyan A,Li K,Li C,et al. Fine-mapping and identifying candidate genes conferring resistance to soybean mosaic virus strain SC20 in soybean. Theoretical and Applied Genetics,2018,131(2):461-476.
[26]Ma F,Wu X,Chen Y,et al. Fine mapping of the Rsv1-h,gene in the soybean cultivar Suweon 97 that confers resistance to two Chinese strains of the soybean mosaic virus. Theoretical and Applied Genetics,2016,129(11):2227-2236.
[27]Sugimoto T,Kaga A,Hajika M,et al. Genetic analysis and identification of DNA markers linked to a novel sojae resistance gene in the Japanese soybean cultivar Waseshiroge. Euphytica,2011,182(1):133-145.
[28]Li N,Zhao M,Liu T,et al. A novel soybean dirigent gene GmDIR22contributes to promotion of lignan biosynthesis and enhances resistance to Phytophthora sojae. Frontiers in Plant Science,2017,8(1):1-8.
[29]Lin F,Zhao M,Ping J,et al. Molecular mapping of two genes conferring resistance to Phytophthora sojae in a soybean landrace PI567139B. Theoretical and Applied Genetics,2013,126(8):2177-2185.
[30]Li L,Feng L,Wang W,et al. Fine mapping and candidate gene analysis of two loci conferring resistance to Phytophthora sojae,in soybean. Theoretical and Applied Genetics,2016,129(12):2379-2386.
[31]Zhang J,Xia C,Duan C,et al. Identification and candidate gene analysis of a novel phytophthora resistance gene Rps10 in a Chinese soybean cultivar. PLoS ONE,2013,8(7):e69799.
[32]Ping J,Fitzgerald J C,Zhang C,et al. Identification and molecular mapping of Rps11,a novel gene conferring resistance to Phytophthora sojae in soybean. Theoretical and Applied Genetics,2016,129(2):445-451.
[33]Zhong C,Sun S,Li Y,et al. Next-generation sequencing to identify candidate genes and develop diagnostic markers for a novel Phytophthora resistance gene,RpsHC18,in soybean. Theoretical and Applied Genetics,2018,131(3):525-538.
[34]Sun J,Li L,Zhao J,et al. Genetic analysis and fine mapping of RpsJS,a novel resistance gene to Phytophthora sojae,in soybean[Glycine max(L.)Merr.]. Theoretical and Applied Genetics,2014,127(4):913-919.
[35]Cheng Y,Ma Q,Ren H,et al. Fine mapping of a Phytophthoraresistance gene RpsWY in soybean(Glycine max L.)by highthroughput genome-wide sequencing. Theoretical and Applied Genetics,2017,130(5):1041-1051.
[36]肖亮,武天龙.大豆抗蚜虫研究进展.中国农学通报,2013,29(36):326-333.
[37]Meng F,Han Y,Teng W,et al. QTL underlying the resistance to soybean aphid(Aphis glycines Matsumura)through isoflavonemediated antibiosis in soybean cultivar'Zhongdou 27'. Theoretical and Applied Genetics,2011,123(8):1459-1465.
[38]Zhang G,Gu D,Wang D. Mapping and validation of a gene for soybean aphid resistance in PI567537. Molecular Breeding,2013,32(1):131-138.
[39]Xiao L,Zhong Y P,Wang B,et al. Mapping an aphid resistance gene in soybean[Glycine max(L.)Merr.] P746. Genetics and Molecular Research,2014,13(4):9152-9160.
[40]Zhang S,Zhang Z,Bales C,et al. Mapping novel aphid resistance QTL from wild soybean,Glycine soja 85-32. Theoretical and Applied Genetics,2017,130(4):1941-1952.
[41]娄雪.野生大豆抗胞囊线虫病相关SNP标记的开发及其辅助选择.长春:东北师范大学,2016.
[42]Chen H,Zhao S,Yang Z,et al. Genetic analysis and molecular mapping of resistance gene to Phakopsora pachyrhizi in soybean germplasm SX6907. Theoretical and Applied Genetics,2015,128(4):733-743.
[43]Liu M,Li S,Swaminathan S,et al. Identification of a soybean rust resistance gene in PI567104B. Theoretical and Applied Genetics,2016,129(5):863-877.
[44]李穆,刘念析,岳岩磊,等.抗大豆白粉病南方栽培大豆种质资源的初步筛选.大豆科学,2016,35(2):209-212.
[45]Wang Y,Shi A,Zhang B,et al. Mapping powdery mildew resistance gene in V97-3000 soybean. Plant Breeding,2013,132(6):625-629.
[46]宋伟,赵雪,徐玲秀,等.大豆抗菌核病位点挖掘及一致性QTL分析.中国油料作物学报,2017,39(6):763-770.
[47]Zhao X,Han Y,Li Y,et al. Loci and candidate gene identification for resistance to Sclerotinia sclerotiorum in soybean[Glycine max(L.)Merr.] via association and linkage maps. The Plant Journal,2015,82(2):245-255.
[2]王艳,韩英鹏,李文滨.大豆分子标记研究新进展.大豆科学,2015,34(1):148-162.
[3]Martin T,Nikolai A,Mugford S G,et al. Combining SNP discovery from next-generation sequencing data with bulked segregant analysis(BSA)to fine-map genes in polyploid wheat. BMC Plant Biology,2012,12(1):12-14.
[4]Liu W Y,Kang J H,Jeong H S,et al. Combined use of bulked segregant analysis and microarrays reveals SNP markers pinpointing a major QTL for resistance to Phytophthora capsici in pepper.Theoretical and Applied Genetics,2014,127(11):2503-2513.
[5]Wu J,Wang Q,Xu L,et al. Combining SNP genotyping array with bulked segregant analysis to map a gene controlling adultplant resistance to stripe rust in wheat line 03031-1-5 H62.Phytopathology,2017,108(1):103-113.
[6]Zhang X,Li R,Chen L,et al. Fine-mapping and candidate gene analysis of the Brassica juncea white-flowered mutant Bjpc2 using the whole-genome resequencing. Molecular Genetics and Genomics,2017,293(2):359-370.
[7]Han Y,Zhao F,Gao S,et al. Fine mapping of a male sterility gene ms-3 in a novel cucumber(Cucumis sativus L.)mutant. Theoretical and Applied Genetics,2018,131(3):449-460.
[8]张红,王超楠,黄志银,等.近等基因系构建及其在育种中应用综述.中国园艺文摘,2018,34(6):54-56.
[9]Kobayashi T,Yamamoto K,Suetsugu Y,et al. Genetic mapping of the rice resistance-breaking gene of the brown planthopper Nilaparvata lugens. Proceedings Biological Sciences,2014,281:296-306.
[10]Chung C L,Poland J,Kump K,et al. Targeted discovery of quantitative trait loci for resistance to northern leaf blight and other diseases of maize. Theoretical and Applied Genetics,2011,123(2):307-326.
[11]Warrington C V,Zhu S,Parrott W A,et al. Seed yield of nearisogenic soybean lines with introgressed quantitative trait loci conditioning resistance to corn earworm(lepidoptera:noctuidae)and soybean looper(lepidoptera:noctuidae)from PI 229358. Journal of Economic Entomology,2008,101(4):1471-1477.
[12]Zheng Z,Ma J,Stiller J,et al. Fine mapping of a large-effect QTL conferring Fusarium crown rot resistance on the long arm of chromosome 3B in hexaploid wheat. BMC Genomics,2015,16(1):1-7.
[13]Cho E K,Goodman R M. Strains of soybean mosaic virus:classification based on virulence in resistant soybean cultivars.Phytopathology,1979,69(5):467-470.
[14]Yulho K,Oksun K,Bongchoon L,et al. G7H,a new soybean mosaic virus strain:its virulence and nucleotide sequence of CI gene. Plant Disease,2003,87(11):1372-1375.
[15]Yang Q H,Gai J Y. Identification,inheritance and gene mapping of resistance to a virulent soybean mosaic virus strain SC15 in soybean.Plant Breeding,2011,130(2):128-132.
[16]Wang D,Zhao L,Li K,et al. Marker-assisted pyramiding of soybean resistance genes RSC4,RSC8,and RSC14Q to soybean mosaic virus. Journal of Integrative Agriculture,2017,16(11):2413-2420.
[17]Ren R,Liu S,Karthikeyan A,et al. Fine-mapping and identification of a novel locus Rsc15,underlying soybean resistance to soybean mosaic virus. Theoretical and Applied Genetics,2017,130(11):2395-2410.
[18]Yan H,Wang H,Cheng H,et al. Detection and fine-mapping of SC7resistance genes via linkage and association analysis in soybean.Journal of Integrative Plant Biology,2015,57(8):722-729.
[19]Zhao L,Wang D,Zhang H,et al. Fine mapping of the RSC8 locus and expression analysis of candidate SMV resistance genes in soybean. Plant Breeding,2016,135(6):701-706.
[20]Zheng G J,Yang Y Q,Ying M A,et al. Fine mapping and candidate gene analysis of resistance gene RSC3Q,to soybean mosaic virus,in Qihuang 1. Journal of Integrative Agriculture,2014,13(12):2608-2615.
[21]Wang D,Ma Y,Liu N,et al. Fine mapping and identification of the soybean RSC4 resistance candidate gene to soybean mosaic virus.Plant Breeding,2011,130(6):653-659.
[22]Yang Y,Zheng G,Han L,et al. Genetic analysis and mapping of genes for resistance to multiple strains of soybean mosaic virus in a single resistant soybean accession PI 96983. Theoretical and Applied Genetics,2013,126(7):1783-1791.
[23]Ma Y,Wang D,Li H,et al. Fine mapping of the RSC14Q,locus for resistance to soybean mosaic virus in soybean. Euphytica,2011,181(1):127-135.
[24]Li K,Ren R,Adhimoolam K,et al. Genetic analysis and identification of two soybean mosaic virus resistance genes in soybean[Glycine max(L.)Merr]. Plant Breeding,2016,134(6):684-695.
[25]Karthikeyan A,Li K,Li C,et al. Fine-mapping and identifying candidate genes conferring resistance to soybean mosaic virus strain SC20 in soybean. Theoretical and Applied Genetics,2018,131(2):461-476.
[26]Ma F,Wu X,Chen Y,et al. Fine mapping of the Rsv1-h,gene in the soybean cultivar Suweon 97 that confers resistance to two Chinese strains of the soybean mosaic virus. Theoretical and Applied Genetics,2016,129(11):2227-2236.
[27]Sugimoto T,Kaga A,Hajika M,et al. Genetic analysis and identification of DNA markers linked to a novel sojae resistance gene in the Japanese soybean cultivar Waseshiroge. Euphytica,2011,182(1):133-145.
[28]Li N,Zhao M,Liu T,et al. A novel soybean dirigent gene GmDIR22contributes to promotion of lignan biosynthesis and enhances resistance to Phytophthora sojae. Frontiers in Plant Science,2017,8(1):1-8.
[29]Lin F,Zhao M,Ping J,et al. Molecular mapping of two genes conferring resistance to Phytophthora sojae in a soybean landrace PI567139B. Theoretical and Applied Genetics,2013,126(8):2177-2185.
[30]Li L,Feng L,Wang W,et al. Fine mapping and candidate gene analysis of two loci conferring resistance to Phytophthora sojae,in soybean. Theoretical and Applied Genetics,2016,129(12):2379-2386.
[31]Zhang J,Xia C,Duan C,et al. Identification and candidate gene analysis of a novel phytophthora resistance gene Rps10 in a Chinese soybean cultivar. PLoS ONE,2013,8(7):e69799.
[32]Ping J,Fitzgerald J C,Zhang C,et al. Identification and molecular mapping of Rps11,a novel gene conferring resistance to Phytophthora sojae in soybean. Theoretical and Applied Genetics,2016,129(2):445-451.
[33]Zhong C,Sun S,Li Y,et al. Next-generation sequencing to identify candidate genes and develop diagnostic markers for a novel Phytophthora resistance gene,RpsHC18,in soybean. Theoretical and Applied Genetics,2018,131(3):525-538.
[34]Sun J,Li L,Zhao J,et al. Genetic analysis and fine mapping of RpsJS,a novel resistance gene to Phytophthora sojae,in soybean[Glycine max(L.)Merr.]. Theoretical and Applied Genetics,2014,127(4):913-919.
[35]Cheng Y,Ma Q,Ren H,et al. Fine mapping of a Phytophthoraresistance gene RpsWY in soybean(Glycine max L.)by highthroughput genome-wide sequencing. Theoretical and Applied Genetics,2017,130(5):1041-1051.
[36]肖亮,武天龙.大豆抗蚜虫研究进展.中国农学通报,2013,29(36):326-333.
[37]Meng F,Han Y,Teng W,et al. QTL underlying the resistance to soybean aphid(Aphis glycines Matsumura)through isoflavonemediated antibiosis in soybean cultivar'Zhongdou 27'. Theoretical and Applied Genetics,2011,123(8):1459-1465.
[38]Zhang G,Gu D,Wang D. Mapping and validation of a gene for soybean aphid resistance in PI567537. Molecular Breeding,2013,32(1):131-138.
[39]Xiao L,Zhong Y P,Wang B,et al. Mapping an aphid resistance gene in soybean[Glycine max(L.)Merr.] P746. Genetics and Molecular Research,2014,13(4):9152-9160.
[40]Zhang S,Zhang Z,Bales C,et al. Mapping novel aphid resistance QTL from wild soybean,Glycine soja 85-32. Theoretical and Applied Genetics,2017,130(4):1941-1952.
[41]娄雪.野生大豆抗胞囊线虫病相关SNP标记的开发及其辅助选择.长春:东北师范大学,2016.
[42]Chen H,Zhao S,Yang Z,et al. Genetic analysis and molecular mapping of resistance gene to Phakopsora pachyrhizi in soybean germplasm SX6907. Theoretical and Applied Genetics,2015,128(4):733-743.
[43]Liu M,Li S,Swaminathan S,et al. Identification of a soybean rust resistance gene in PI567104B. Theoretical and Applied Genetics,2016,129(5):863-877.
[44]李穆,刘念析,岳岩磊,等.抗大豆白粉病南方栽培大豆种质资源的初步筛选.大豆科学,2016,35(2):209-212.
[45]Wang Y,Shi A,Zhang B,et al. Mapping powdery mildew resistance gene in V97-3000 soybean. Plant Breeding,2013,132(6):625-629.
[46]宋伟,赵雪,徐玲秀,等.大豆抗菌核病位点挖掘及一致性QTL分析.中国油料作物学报,2017,39(6):763-770.
[47]Zhao X,Han Y,Li Y,et al. Loci and candidate gene identification for resistance to Sclerotinia sclerotiorum in soybean[Glycine max(L.)Merr.] via association and linkage maps. The Plant Journal,2015,82(2):245-255.