福建省部分规模猪场猪圆环病毒病疫苗免疫效果评价与分析
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摘要
为了解免疫不同圆环病毒病疫苗的抗体水平,给养殖户提供合适的免疫依据,对福建省不同地区8个猪场在使用不同猪圆环病毒病疫苗和免疫程序后猪群的抗体水平进行检测。结果显示:免疫场和未免疫场各阶段种猪群的猪圆环病毒病抗体阳性率皆为100%,差异不显著(P>0.05);但各阶段种猪群的S/P平均值,免疫场较未免疫场更高;同时通过比较变异系数发现,未免疫场种猪的抗体水平更活跃;14日龄免疫亚单位疫苗后抗体水平较高且维持时间较长,而免疫全病毒灭活苗后抗体水平仍持续下降。综上,种猪群免疫圆环病毒病疫苗是必要的,亚单位疫苗比全病毒灭活苗的免疫效果好。
In order to understand the antibody level of different circoidal vaccines and provide appropriate basis for farmers' circoidal immunity.The antibody levels of pigs after different immunization programs using different porcine circovirus vaccines were tested in 8 pig farms in different areas of Fujian province. The result showed that the pigs in the immunization field and the non-immunized field were positive for the pig ring antibody. The rates were all 100%, and the difference was not significant(P>0.05); however, the average S/P of the pigs at each stage was higher than that of the non-immunized field; and the antibody coefficient of the unimmunized field of the pigs was found to be more active by comparing the coefficient of variation. the antibody level of the 14-day-old immunized subunit vaccine was higher and the maintenance time was longer, and the antibody level continued to decrease after the immunization of the whole virus inactivated vaccine. It was found that it is necessary to breed the immune circovirus vaccine of pigs. The immune effect of porcine circovirus subunit vaccine is better than that of whole virus inactivated vaccine. The porcine circovirus type 2 ELISA antibody detection kit used in this experiment can reflect the antibody of artificial immunity and wild-type infection.
In order to understand the antibody level of different circoidal vaccines and provide appropriate basis for farmers' circoidal immunity.The antibody levels of pigs after different immunization programs using different porcine circovirus vaccines were tested in 8 pig farms in different areas of Fujian province. The result showed that the pigs in the immunization field and the non-immunized field were positive for the pig ring antibody. The rates were all 100%, and the difference was not significant(P>0.05); however, the average S/P of the pigs at each stage was higher than that of the non-immunized field; and the antibody coefficient of the unimmunized field of the pigs was found to be more active by comparing the coefficient of variation. the antibody level of the 14-day-old immunized subunit vaccine was higher and the maintenance time was longer, and the antibody level continued to decrease after the immunization of the whole virus inactivated vaccine. It was found that it is necessary to breed the immune circovirus vaccine of pigs. The immune effect of porcine circovirus subunit vaccine is better than that of whole virus inactivated vaccine. The porcine circovirus type 2 ELISA antibody detection kit used in this experiment can reflect the antibody of artificial immunity and wild-type infection.
引文
[1]董春霞,凌洪权,黎朝燕,等.猪圆环病毒病及其危害[J].中国畜牧兽医文摘,2014,30(12):117-118.
[2]芦银华,谈国蕾,华修国,等.应用间接免疫荧光试验检测猪圆环病毒抗体[J].中国兽医科技,2002,32(8):19-20.
[3]于新友,李天芝,李峰,等.猪圆环病毒病流行与防治进展[J].猪业科学,2015,32(11):100-102.
[4]史岩,秦爱建.猪圆环病毒2型生物学特性与致病特征研究进展[J].扬州大学报,2010,31(1):10-15.
[5]刘长明,陆月华,张超范,等.猪圆环病毒2型重组Cap蛋白在昆虫杆状病毒中的表达[J].中国预防兽医学报,2005,27(26):479-482.
[6]琚春梅,陈焕春,樊惠英,等,猪2型圆环病毒ORF1与ORF2基因和伪狂犬病毒基因的重组与表达的研究[J].生物工程学报,2005,21(3):370-374.
[7]刘新文,姚清侠,曹胜波,等.嵌合猪圆环病毒PVC2-1感染性克隆的构建[J].中国兽医学报,2007,21(1):1-5.
[8] Pallares F J,Halbur P G,Opriessnig T,et al.Porcine circovirus type 2(PCV2)coinfections in US field cases of postweaning multisystemic wasting syndrome(PMWS)[J].Journal of Veterinary Diagnostic Investigation,2002,14(6):515-519.
[9]郏建中.动物防疫在畜牧业中的地位作用与发展思路[J].内蒙古兽医,2011(1):24-25.
[10]彭志峰.河南省2型圆环病毒分子流行病学调查[D].郑州:河南农业大学,2009.
[11]杨芳,陈立功,李艳琴,等.猪圆环病毒2型SD/2008株对仔猪的致病性[J].中国兽医学报,2013,33(4):538-544.
[12]郎洪武,王力,张广川,等.猪圆环病毒分离鉴定及猪断乳多系统衰弱综合征的诊断[J].中国兽医科技,2001,31(3):3-5.
[13] Desrosicrs R,温鹏.猪圆环病毒病(PCVD)概况-猪圆环病毒病在加拿大东部、欧洲和美国的情况[J].今日养猪业,2008(1):16-20.
[14]潘群兴,陈德,何孔旺,等.检测PCV2、PPV、PRV疫苗株与野毒株的多重PCR方法[J].中国病毒学,2005,20(6):603-606.
[2]芦银华,谈国蕾,华修国,等.应用间接免疫荧光试验检测猪圆环病毒抗体[J].中国兽医科技,2002,32(8):19-20.
[3]于新友,李天芝,李峰,等.猪圆环病毒病流行与防治进展[J].猪业科学,2015,32(11):100-102.
[4]史岩,秦爱建.猪圆环病毒2型生物学特性与致病特征研究进展[J].扬州大学报,2010,31(1):10-15.
[5]刘长明,陆月华,张超范,等.猪圆环病毒2型重组Cap蛋白在昆虫杆状病毒中的表达[J].中国预防兽医学报,2005,27(26):479-482.
[6]琚春梅,陈焕春,樊惠英,等,猪2型圆环病毒ORF1与ORF2基因和伪狂犬病毒基因的重组与表达的研究[J].生物工程学报,2005,21(3):370-374.
[7]刘新文,姚清侠,曹胜波,等.嵌合猪圆环病毒PVC2-1感染性克隆的构建[J].中国兽医学报,2007,21(1):1-5.
[8] Pallares F J,Halbur P G,Opriessnig T,et al.Porcine circovirus type 2(PCV2)coinfections in US field cases of postweaning multisystemic wasting syndrome(PMWS)[J].Journal of Veterinary Diagnostic Investigation,2002,14(6):515-519.
[9]郏建中.动物防疫在畜牧业中的地位作用与发展思路[J].内蒙古兽医,2011(1):24-25.
[10]彭志峰.河南省2型圆环病毒分子流行病学调查[D].郑州:河南农业大学,2009.
[11]杨芳,陈立功,李艳琴,等.猪圆环病毒2型SD/2008株对仔猪的致病性[J].中国兽医学报,2013,33(4):538-544.
[12]郎洪武,王力,张广川,等.猪圆环病毒分离鉴定及猪断乳多系统衰弱综合征的诊断[J].中国兽医科技,2001,31(3):3-5.
[13] Desrosicrs R,温鹏.猪圆环病毒病(PCVD)概况-猪圆环病毒病在加拿大东部、欧洲和美国的情况[J].今日养猪业,2008(1):16-20.
[14]潘群兴,陈德,何孔旺,等.检测PCV2、PPV、PRV疫苗株与野毒株的多重PCR方法[J].中国病毒学,2005,20(6):603-606.