东北褐环乳牛肝菌多糖提取及抗氧化活性研究
|
摘要
以褐环乳牛肝菌为原料,采用水提-醇沉法从东北产褐环乳牛肝菌中提取子实体多糖,并对粗多糖进行体外抗氧化研究。结果表明:其多糖对超氧离子自由基(·O~(2-))的清除率为48.30%;多糖对羟基自由基(·OH)的清除率为62.19%;多糖对DPPH自由基的清除率为28.32%,其红外表征显示多糖为β-型吡喃多糖。其显示出多糖有一定抗氧化能力,具有一定的开发前景。
The Suillus luteus was raw material,the polysaccharide was extracted from Suillus luteus by water extracting-alcohol precipitating. In vitro antioxidant systems, the antioxidant activity of polysaccharide on Suillus luteus were discussed. The results showed that: The scavenging rate of superoxide radical was 48.30%,DPPH was 28.32%,hydroxyl radical was 62.19%.in a word, the IR showed that polysaccharide is beta-pyranose. Suillus luteus' polysaccharide has a certain antioxidant capacities and a bright future.
The Suillus luteus was raw material,the polysaccharide was extracted from Suillus luteus by water extracting-alcohol precipitating. In vitro antioxidant systems, the antioxidant activity of polysaccharide on Suillus luteus were discussed. The results showed that: The scavenging rate of superoxide radical was 48.30%,DPPH was 28.32%,hydroxyl radical was 62.19%.in a word, the IR showed that polysaccharide is beta-pyranose. Suillus luteus' polysaccharide has a certain antioxidant capacities and a bright future.
引文
[1]尹大川,周洪敏,高慈元,等.褐环乳牛肝菌的生物学特性研究[J].沈阳农业大学学报,2017,48(03):348-353.
[2]刘虹,李素玲,许晶,等.山西吕梁山地区乳牛肝菌属物种多样性研究[J].中国食用菌,2018,37(06):15-19.
[3]韩长志,任雪敏.褐环乳牛肝菌的研究进展[J].北方园艺,2016(06):195-199.
[4]宫雪. 不同干燥方式褐环乳牛肝菌风味成分分析及电子舌感官评价[D]. 沈阳:沈阳农业大学,2017.
[5]王立安. 黄乳牛肝菌子实体中含丰富的抗氧化活性物质[C].中国菌物学会学术年会论文集,中国湖南张家界.中国菌物学会,2010:3.
[6]顾可飞,周昌艳,李晓贝.食用菌的营养价值及药用价值[J].食品工业,2017,38(10):228-231.
[7]员子晶,姚庆智,闫伟.褐环乳牛肝菌对油松根际土壤可培养微生物数量影响的研究[J].内蒙古农业大学学报(自然科学版),2011,32(02):175-178.
[8]尹大川,宋瑞清,祁金玉,等.共培养条件下绿木霉对褐环乳牛肝菌产酶的诱导效应[J].菌物学报,2018,37(05):617-624.
[9]祁金玉,邓继峰,尹大川,等.外生菌根菌对油松幼苗抗氧化酶活性及根系构型的影响[J].生态学报,2019(08):1-6.
[10]李敏,闫伟,堵国成,等.褐环乳牛肝菌发酵条件的优化[J].食品与生物技术学报,2009,28(03):390-396.
[11]刘刚,厍守权,高文爽,等.长白山5种真菌粗提取物的抗氧化作用[J].延边大学医学学报,2013,36(01):16-21.
[12]刘品华,徐方,刘明研,等云南十三种野生菌粗多糖含量和抗氧化活性的研究[J].保鲜与加工,2017,17(06):78-84.
[13]王丽梅,叶诚,吴晨,等.紫苏油对衰老模型大鼠的抗衰老作用研究[J].食品科技,2013,38(01):280-284.
[14]魏梦涛,徐佳,舒青龙,等.紫草素清除自由基和抑制脂质过氧化活性研究[J].食品工业,2018,39(03):140-143.
[15]何婷婷,柴军红,金志民,等.软枣猕猴桃多糖、黄酮提取工艺的优化及抗氧化活性[J].江苏农业科学,2017,45(21):199-201.
[16]柴军红,何婷婷,金志民,等.黑加仑果渣提取物抗氧化活性研究[J].食品研究与开发,2018,39(01):16-20.
[17]KARAKA.Didem,ARI, Ferda,ULUKAYA, Engin.The MTT viability assay yields strikingly false-positive viabilities although the cells are killed by some plant extracts[J]. Turkish Journal of Biology,2017, 41(06):919-925.
[18]Liyan LI,Tao HUANG.Growth Inhibitory Effects of Garlic Polysaccharide on Human HepG2 Cells[J]. Agricultural Science & Technology,2017,18(06):988-992.5.
[19]蒋增良,刘晓庆,王珍珍,等.葡萄酵素有机酸分析及其体外抗氧化性能[J].中国食品学报,2017,17(05):255-262.
[20]张镜,温思霞,廖富林,等. 温度、光照及pH值对阴香花色苷清除DPPH自由基活性的影响[J].食品科学, 2009,30(13):120-123.
[21]余兰彬,徐国良,谭志伟,等. DPPH 法对5 种抗衰老中药抗氧化活性的研究[J].江西中医药,2016,47(08):74-76.
[22]魏元.香菇多糖的化学结构与抗氧化活性分析[J].江苏农业科学,2016,44(11):305-308.
[2]刘虹,李素玲,许晶,等.山西吕梁山地区乳牛肝菌属物种多样性研究[J].中国食用菌,2018,37(06):15-19.
[3]韩长志,任雪敏.褐环乳牛肝菌的研究进展[J].北方园艺,2016(06):195-199.
[4]宫雪. 不同干燥方式褐环乳牛肝菌风味成分分析及电子舌感官评价[D]. 沈阳:沈阳农业大学,2017.
[5]王立安. 黄乳牛肝菌子实体中含丰富的抗氧化活性物质[C].中国菌物学会学术年会论文集,中国湖南张家界.中国菌物学会,2010:3.
[6]顾可飞,周昌艳,李晓贝.食用菌的营养价值及药用价值[J].食品工业,2017,38(10):228-231.
[7]员子晶,姚庆智,闫伟.褐环乳牛肝菌对油松根际土壤可培养微生物数量影响的研究[J].内蒙古农业大学学报(自然科学版),2011,32(02):175-178.
[8]尹大川,宋瑞清,祁金玉,等.共培养条件下绿木霉对褐环乳牛肝菌产酶的诱导效应[J].菌物学报,2018,37(05):617-624.
[9]祁金玉,邓继峰,尹大川,等.外生菌根菌对油松幼苗抗氧化酶活性及根系构型的影响[J].生态学报,2019(08):1-6.
[10]李敏,闫伟,堵国成,等.褐环乳牛肝菌发酵条件的优化[J].食品与生物技术学报,2009,28(03):390-396.
[11]刘刚,厍守权,高文爽,等.长白山5种真菌粗提取物的抗氧化作用[J].延边大学医学学报,2013,36(01):16-21.
[12]刘品华,徐方,刘明研,等云南十三种野生菌粗多糖含量和抗氧化活性的研究[J].保鲜与加工,2017,17(06):78-84.
[13]王丽梅,叶诚,吴晨,等.紫苏油对衰老模型大鼠的抗衰老作用研究[J].食品科技,2013,38(01):280-284.
[14]魏梦涛,徐佳,舒青龙,等.紫草素清除自由基和抑制脂质过氧化活性研究[J].食品工业,2018,39(03):140-143.
[15]何婷婷,柴军红,金志民,等.软枣猕猴桃多糖、黄酮提取工艺的优化及抗氧化活性[J].江苏农业科学,2017,45(21):199-201.
[16]柴军红,何婷婷,金志民,等.黑加仑果渣提取物抗氧化活性研究[J].食品研究与开发,2018,39(01):16-20.
[17]KARAKA.Didem,ARI, Ferda,ULUKAYA, Engin.The MTT viability assay yields strikingly false-positive viabilities although the cells are killed by some plant extracts[J]. Turkish Journal of Biology,2017, 41(06):919-925.
[18]Liyan LI,Tao HUANG.Growth Inhibitory Effects of Garlic Polysaccharide on Human HepG2 Cells[J]. Agricultural Science & Technology,2017,18(06):988-992.5.
[19]蒋增良,刘晓庆,王珍珍,等.葡萄酵素有机酸分析及其体外抗氧化性能[J].中国食品学报,2017,17(05):255-262.
[20]张镜,温思霞,廖富林,等. 温度、光照及pH值对阴香花色苷清除DPPH自由基活性的影响[J].食品科学, 2009,30(13):120-123.
[21]余兰彬,徐国良,谭志伟,等. DPPH 法对5 种抗衰老中药抗氧化活性的研究[J].江西中医药,2016,47(08):74-76.
[22]魏元.香菇多糖的化学结构与抗氧化活性分析[J].江苏农业科学,2016,44(11):305-308.