喹啉高效降解菌株Alcaligenes sp. WUST-qu的筛选、鉴定及降解特性
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摘要
从某焦化厂污水处理系统的活性污泥中筛选到一株喹啉高效降解菌WUST-qu,对其形态、分类地位、降解特征和生长动力学进行表征。结果表明,该菌株为革兰氏阴性菌,有芽孢,长为1.15μm±0.08μm(n=20),宽为0.26μm±0.01μm (n=20);16S rRNA基因核苷酸序列比对结果表明,该菌株属产碱杆菌属微生物(Alcaligenes sp)。菌株WUST-qu能在初始pH为5.0～9.0内有效降解喹啉,最适初始pH为中性到略偏碱性(7.0～8.0)。在该条件下,菌株能在接种后的48 h内将最高初始质量浓度为700 mg·L~(-1)的喹啉完全降解。进一步研究表明,菌株WUST-qu不仅能够有效降解喹啉,还能有效降解苯酚,在MSM初始pH为8.0的条件下,菌株WUST-qu能在32 h内将初始质量浓度为700 mg·L~(-1)的苯酚完全降解。其以喹啉为唯一碳源生长时的动力学参数为μ_(max)=1.657 6 h~(-1),K_s=36.42 mg·L~(-1),K_i=81.418 mg·L~(-1)。
In this study,a potent quinoline-degrading bacterial strain WUST-qu was isolated from the activated sludge of the wastewater treatment system of a coke plant,and the morphological properties,taxonomic status,degrading characteristics,and kinetics of growth were characterized. The results demonstrated that the strain WUST-qu is Gram negative and sporulating with a length of 1.15 μm ±0.08 μm(n=20),and a width of 0.26 μm ±0.01 μm(n=20). Partial 16 S rDNA sequence alignment indicated that WUST-qu belongs to the genus of Alcaligenes.The strain could effectively degrade quinoline at between pH 5.0 and 9.0,but the ideal pH to degrade quinoline is neutral or slightly basic(7.0-8.0),under which the strain could completely degrade quinoline at a maximal initial concentration of 700 mg·L~(-1) within 48 h upon inoculation. The further study indicated that the strain WUST-qu also degrades phenol effectively. The strain could completely degrade phenol at 700 mg·L~(-1) within 32 h at an initial pH of 8.0. The kinetic parameters for growth with quinoine as the sole carbon were as follow: μ_(max)= 1.657 6 h~(-1),K_s= 36.42 mg·L~(-1),K_i= 81.418 mg·L~(-1).
In this study,a potent quinoline-degrading bacterial strain WUST-qu was isolated from the activated sludge of the wastewater treatment system of a coke plant,and the morphological properties,taxonomic status,degrading characteristics,and kinetics of growth were characterized. The results demonstrated that the strain WUST-qu is Gram negative and sporulating with a length of 1.15 μm ±0.08 μm(n=20),and a width of 0.26 μm ±0.01 μm(n=20). Partial 16 S rDNA sequence alignment indicated that WUST-qu belongs to the genus of Alcaligenes.The strain could effectively degrade quinoline at between pH 5.0 and 9.0,but the ideal pH to degrade quinoline is neutral or slightly basic(7.0-8.0),under which the strain could completely degrade quinoline at a maximal initial concentration of 700 mg·L~(-1) within 48 h upon inoculation. The further study indicated that the strain WUST-qu also degrades phenol effectively. The strain could completely degrade phenol at 700 mg·L~(-1) within 32 h at an initial pH of 8.0. The kinetic parameters for growth with quinoine as the sole carbon were as follow: μ_(max)= 1.657 6 h~(-1),K_s= 36.42 mg·L~(-1),K_i= 81.418 mg·L~(-1).
引文
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[13] ACIKGOZ E,OZCAN B.Phenol biodegradation by Halophilic archaea[J].International Biodeterioration & Biodegradation,2016,107:140-146.
[14] 李静,李文英.喹啉降解菌筛选及其对焦化废水强化处理[J].环境科学,2015,36(4):1385-1391. LI J,LI W Y.Screening of a highly efficient quinoline-degrading strain and its enhanced biotreatment on coking waste water[J].Environmental Science,2015,36(4):1385-1391.
[2] SUN Q H,BAI Y H,ZHAO C,et al.Aerobic biodegradation characteristics and metabolic products of quinoline by a Pseudomonas strain[J].Bioresource Technology,2009,100(21):5030-5036.
[3] 朴铁夫,高山,李国全,等.4-硝基喹啉-1-氧化物对小麦损伤效应的研究[J].吉林农业大学学报,1995,17(3):26-28. PIAO T F,GAO SH,LI G Q,et al.Studies on damage effect of 4-NQO to wheat[J].Journal of Jilin Agricultural University,1995,17(3):26-28.
[4] 朴铁夫,韩秀云,原亚萍,等.4-NQO对蚕豆损伤效应的研究[J].核农学通报,1995,16(4):186-187. PIAO T F,HAN X Y,YUAN Y P,et al.Studies on damage effect of 4-NQO to horsebean[J].Nuclear Agriculture Bulletin,1995,16(4):186-187.
[5] 陈万涛.4-硝基喹啉-1-氧化物诱发大鼠舌黏膜鳞癌模型和分子发病机制研究进展[J].中国实用口腔科杂志,2010,3(7):387-390. CHEN W T.The animal model with tongue squamous cell carcinoma induced by 4-NQO and its mechanism of molecular carcinogenesis[J].Chinese Journal of Practical Stomatology,2010,3(7):387-390.
[6] OBEROI A S,PHILIP L,BHALLAMUDI S M.Biodegradation of various aromatic compounds by enriched bacterial cultures: part B-nitrogen-,sulfur-,and oxygen-containing heterocyclic aromatic compounds[J].Applied Biochemistry Biotechnology,2015,176:1746-1769.
[7] 沈萍,陈向东.微生物学实验[M].北京:高等教育出版社,2007. SHEN P,CHEN X D.Microbiology Experiments[M].Beijing:Higher Education Press,2007.
[8] LIU J ZH,WANG Q,YAN J B,et al.Isolation and characterization of a novel phenol degrading bacterial strain WUST-C1[J].Industrial & Engineering Chemistry Research,2013,52(1):258-265.
[9] 郑中原,赵翠,温东辉,等.喹啉降解菌BW004的分离、鉴定及降解特性[J].北京大学学报(自然科学版),2013,49(4):683-688. ZHENG ZH Y,ZHAO C,WEN D H,et al.Isolation,identification,and metabolic characteristics of a quinoline degrading bacterium[J].Acta Scientiarum Naturalium Universitatis Pekinensis,2013,49(4):683-688.
[10] ZHU S N,LIU D Q,FAN L,et al.Degradation of quinoline by Rhodococcus sp.QL2 isolated from activated sludge[J].Journal of Hazardous Materials,2008,160(23):289-294.
[11] WANG J L,QUAN X CH,HAN L P,et al.Microbial degradation of quinoline by immobilized cells of Burkholderia pickettii[J].Water Research,2002,36(9):2288-2296.
[12] STOBDAN T,SINHA A,SINGH R P,et al.Degradation of pyridine and 4-methylpyridine by Gordonia terrea IIPN1[J].Biodegradation,2008,19(4):481-487.
[13] ACIKGOZ E,OZCAN B.Phenol biodegradation by Halophilic archaea[J].International Biodeterioration & Biodegradation,2016,107:140-146.
[14] 李静,李文英.喹啉降解菌筛选及其对焦化废水强化处理[J].环境科学,2015,36(4):1385-1391. LI J,LI W Y.Screening of a highly efficient quinoline-degrading strain and its enhanced biotreatment on coking waste water[J].Environmental Science,2015,36(4):1385-1391.