家蚕茧丝相关性状QTLs和无鳞毛翅基因nlw的定位
摘要
分子标记技术是基因组研究的有力工具,目前在家蚕功能基因定位及育种方面得到了广泛应用。本研究主要采用第二代分子标记SSR(Simple sequence repeats)和STS(Sequence-tagged sites)对家蚕茧丝性状相关的QTLs(Quantitative trait locus)及无鳞毛翅基因进行了定位,并用第三代分子标记SNP(Single Nucleotide Polymorphisms)进行了部分连锁群的初步定位分析。主要研究结果如下:
采用茧丝成绩差异较大的家蚕品种L10和Js组配分离群体,结合本实验室的相关实验数据,有针对性地对第一号染色体进行了连锁分析及QTL位点的扫描,构建了含有17个多态标记的遗传连锁图,得到了与茧丝性状相关的QTLs位点。
采用正常翅P50和无鳞毛翅U06两个亲本组配分离群体,利用BC1F群体得到了8个与家蚕无鳞毛翅nlw基因连锁的SSR标记和1个STS标记,采用BC1M群体构建了关于nlw基因的遗传连锁图,连锁图的遗传距离为125.7 cM,与nlw基因最近的引物为STS标记cash2p,图距为11.4cM。
利用L10和Js组配的分离群体对SNP分子标记进行了研究分析,建立了实用、经济的SNP检测平台,为SNP的广泛应用奠定了基础。
Molecular makers are powerful tool in the analysis of genome, which are comprehensively applied in mapping functional genes and molecular breeding of silkworm (Bombyx mori). SSR(Simple sequence repeats)and STS (Sequence-tagged sites) were adopted in this project to map QTLs (Quantitative trait locus) for silk quality and non-lepis wing gene, nlw. Meanwhile, SNP (Single Nucleotide Polymorphisms) makers were employed in mapping analysis of the eighteenth chromosome in the silkworm. The main results as follows:
The significant divergence exists in the silk quality of silkworm strains Js and L10. A backcross population (BC1) was bred using Js and L10 as parents. Base on the former experimental data of Zhan et al (2009), there was a QTL located at the first chromosome. A linkage map containing seventeen polymorphic makers was constructed and the QTLs relation to silk characters were mapped.
The reciprocal backcrossed F1 (BC1) progenies were constructed using the wild type (+nlw/+nlw) silkworm strain P50 and U06 with scaleless wing (nlw/nlw). The gene of interest was linked to eight SSR markers and one STS marker.A linkage map of 125.7 cM was constructed using a reciprocal BC1M population, and the distance between nlw and the nearest marker cash2p was 11.4 cM.
SNP makers were analysed using backcross population (BC1) derived from L10 and Js, and the economical detection platform for SNP were established, which lay the foundation for the mass application of SNP.
采用茧丝成绩差异较大的家蚕品种L10和Js组配分离群体,结合本实验室的相关实验数据,有针对性地对第一号染色体进行了连锁分析及QTL位点的扫描,构建了含有17个多态标记的遗传连锁图,得到了与茧丝性状相关的QTLs位点。
采用正常翅P50和无鳞毛翅U06两个亲本组配分离群体,利用BC1F群体得到了8个与家蚕无鳞毛翅nlw基因连锁的SSR标记和1个STS标记,采用BC1M群体构建了关于nlw基因的遗传连锁图,连锁图的遗传距离为125.7 cM,与nlw基因最近的引物为STS标记cash2p,图距为11.4cM。
利用L10和Js组配的分离群体对SNP分子标记进行了研究分析,建立了实用、经济的SNP检测平台,为SNP的广泛应用奠定了基础。
Molecular makers are powerful tool in the analysis of genome, which are comprehensively applied in mapping functional genes and molecular breeding of silkworm (Bombyx mori). SSR(Simple sequence repeats)and STS (Sequence-tagged sites) were adopted in this project to map QTLs (Quantitative trait locus) for silk quality and non-lepis wing gene, nlw. Meanwhile, SNP (Single Nucleotide Polymorphisms) makers were employed in mapping analysis of the eighteenth chromosome in the silkworm. The main results as follows:
The significant divergence exists in the silk quality of silkworm strains Js and L10. A backcross population (BC1) was bred using Js and L10 as parents. Base on the former experimental data of Zhan et al (2009), there was a QTL located at the first chromosome. A linkage map containing seventeen polymorphic makers was constructed and the QTLs relation to silk characters were mapped.
The reciprocal backcrossed F1 (BC1) progenies were constructed using the wild type (+nlw/+nlw) silkworm strain P50 and U06 with scaleless wing (nlw/nlw). The gene of interest was linked to eight SSR markers and one STS marker.A linkage map of 125.7 cM was constructed using a reciprocal BC1M population, and the distance between nlw and the nearest marker cash2p was 11.4 cM.
SNP makers were analysed using backcross population (BC1) derived from L10 and Js, and the economical detection platform for SNP were established, which lay the foundation for the mass application of SNP.
引文
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