中国野生葡萄芪合成酶基因的克隆及转化欧洲葡萄研究
|
摘要
葡萄（Vitis spp.）是世界性重要的经济作物，常遭受真菌病的危害。葡萄白藜芦醇是一种重要的植保素，在植物对病原菌的防御反应中起重要作用，同时又对人体健康具有消炎、抗癌、预防心脑血管疾病等营养保健作用。芪合成酶是在葡萄中催化合成白藜芦醇的关键酶。本研究克隆了中国野生华东葡萄株系‘白河-35-1’(Vitis pseudoreticulataaccession Baihe-35-1)抗白粉病芪合成酶基因，克隆了野生毛葡萄株系‘丹凤-2’(Vitisquinquangularis accession Danfeng-2)抗白粉病芪合成酶基因，并对其进行了生物信息学分析及转化研究。取得的结果如下：
1．通过RACE技术克隆获得在病原菌诱导下的中国野生华东葡萄株系‘白河-35-1’芪合成酶基因家族成员53条，其中4条序列编码区有缺失，为假基因。将获得的芪合成酶基因序列登入GenBank，登录号为：JQ886584至JQ886636。欧洲葡萄和中国野生华东葡萄株系‘白河-35-1’芪合成酶基因部分序列同源最高达98%，但聚类亲缘关系相对较远。
2．采用RT-PCR技术克隆中国野生毛葡萄株系‘丹凤-2’芪合成酶基因VqDSTS1，并进行了序列及表达模式分析。结果表明，VqDSTS1基因cDNA编码区全长为1179bp，GenBank登录号为JQ342086,编码392个氨基酸；氨基酸序列分析表明，VqDSTS1含有芪合成酶基因家族的特征识别序列‘IPNSAGAIAGN’和‘GVLFGFGPGLT’；序列比对显示VqDSTS1与其他葡萄种质的芪合成酶氨基酸序列一致性在95.2%~98.7%之间；半定量RT-PCR分析表明，VqDSTS1受白粉病诱导表达，呈双峰模式。为进一步研究中国野生毛葡萄株系‘丹凤-2’芪合成酶基因家族的表达及功能分析提供了基础。
3．在欧洲葡萄胚状体再生体系中，‘红地球’花药的最佳诱导培养基为NN69+CH0.5g·L~(-1)+PVP1g·L~(-1)＋2,4-D0.5mg·L~(-1)+BAP4.0mg·L~(-1)；子房的最佳诱导培养基为NN69+CH0.5g·L~(-1)+PVP1g·L~(-1)＋2,4-D1.0mg·L~(-1)+BAP4.0mg·L~(-1)。赤霞珠的花器官胚性愈伤组织的诱导效率要明显高于无核白。NN69培养基的诱导率最高（花药29.3%，子房32.5%），其次是B5培养基（花药4.5%，子房9.1%），MS培养基诱导率最低（花药4.0%，子房6.3%）。细胞悬浮培养的方法可迅速扩繁胚性愈伤组织，且能够获得均一的体细胞胚。
4．农杆菌介导的遗传转化中，潮霉素对愈伤组织的最低致死浓度为13mg·L~(-1)；采用此浓度对胚性愈伤组织进行抗性筛选。GV3101菌株转化率明显高于LBA4404菌株。转化后抗性愈伤组织随着继代次数的增加，报告基因GFP的表达逐渐增强。
The grapevine is one of the most important fruit crops in the world, which are generallysusceptible to Erysiphe necator and Plasmopara viticola. Stilbene synthase (STS) is the keyenzyme for producing the resveratrol. Grape resveratrol is a phytoalexin, plays an importantrole in plant defense responses to pathogens, and yet also shows significant health-promotingeffects including anti-cancer, prevention of cardiovascular disease and neuroprotectiveproperties. In this study, stilbene synthase genes were cloned from Chinese wild V.pseudoreticulata accession ‘Baihe-35-1’ which showed high resistance to powdery mildewand downy mildew. A stilbene synthase gene was clone from Chinese wild V. quinquangularisaccession ‘Danfeng-2’ which showed high resistance to powdery mildew and high content ofresveratrol in mature berries; Bioinformatics analysis and the study of transformation wereperformed in this study. The main results are described as followings.
1．STS sequences covered all coding region and3’ UTR were cloned from E. necatorinoculated-leaves of Chinese wild V. pseudoreticulata accession ‘Baihe-35-1’ by RACEamplification. RT-PCR products were analyzed by gel electrophoresis, cloned and more than800individual clones were sequenced. Sequence analysis identified that ‘Baihe-35-1’ have53single STS genes, four of which were pseudogene which do not encode stilbene synthasecorrectly. GenBank Accession numbers of STS from V. pseudoreticulata were from JQ886584to JQ886636. STS sequences from V. vinifera and V. pseudoreticulata sequences werehomologies, which the highest level can reach98%, but most of them cannot be grouped in asingle cluster.
2．VqDSTS1, was isolated by reverse transcription-polymerase chain reaction (RT-PCR)from Chinese wild Vitis quinquangularis accession 'Danfeng-2', and then analysed thesequence and expression model. The results suggested the full length cDNA sequence ofVqDSTS1was1179bp, and the accession number of VqDSTS1was JQ342086, and encoded392amino acid residues. Detailed analysis of the amino acid sequence revealed thatVqDSTS1contains "IPNSAGAIAGN" and "GVLFGFGPGLT" which can be found in allstilbene synthase gene family. With alignment of amino acid sequences, VqDSTS1shared highly identity between95.2%~98.7%with the stilbene synthase from other Vitis. From theanalysis of semi-quantitative, it was found that the expression model of VqDSTS1hadtwin-peak model. This work would make a foundation to investigate the function of stilbenesynthase gene family from Chinese wild Vitis quinquangularis accession 'Danfeng-2'.
3．During the construct process of the system of somatic embryos from Vitis vinifera, itwas found that the best induction medium was NN69+CH0.5g·L~(-1)+PVP1g·L~(-1)＋2,4-D0.5mg·L~(-1)+BAP4.0mg·L~(-1)from anthers of ‘Red Globe’; for the overies of ‘Red Globe’,NN69+CH0.5g·L~(-1)+PVP1g·L~(-1)＋2,4-D1.0mg·L~(-1)+BAP4.0mg·L~(-1). The embryo callusinduction rate from ‘Cabernet Sauvignon’ was obviously higher than the from ‘ThompsonSeedless’. The induction rate of NN69basic medium was the highest (anthers29.3%, overies32.5%); B5for the second (anthers4.5%, overies9.1%); MS the lowest (anthers4.0%, overies6.3%). The method of cell suspension culture could quickly proliferate the embryo callus cells,and then get the same somatic embryos.
4．During the agrobacterium-mediated transformation, the lethal concentration of Hygwas mg·L~(-1), efficiently when using this concentration of Hyg in the process of selecting theresistant callus. The tansformation rate of GV3101was obviously higher than LBA4404. Theexpression of report gene GFP was ever-increasing, when the resistant callus was sub-culturedfor several times.
1．通过RACE技术克隆获得在病原菌诱导下的中国野生华东葡萄株系‘白河-35-1’芪合成酶基因家族成员53条，其中4条序列编码区有缺失，为假基因。将获得的芪合成酶基因序列登入GenBank，登录号为：JQ886584至JQ886636。欧洲葡萄和中国野生华东葡萄株系‘白河-35-1’芪合成酶基因部分序列同源最高达98%，但聚类亲缘关系相对较远。
2．采用RT-PCR技术克隆中国野生毛葡萄株系‘丹凤-2’芪合成酶基因VqDSTS1，并进行了序列及表达模式分析。结果表明，VqDSTS1基因cDNA编码区全长为1179bp，GenBank登录号为JQ342086,编码392个氨基酸；氨基酸序列分析表明，VqDSTS1含有芪合成酶基因家族的特征识别序列‘IPNSAGAIAGN’和‘GVLFGFGPGLT’；序列比对显示VqDSTS1与其他葡萄种质的芪合成酶氨基酸序列一致性在95.2%~98.7%之间；半定量RT-PCR分析表明，VqDSTS1受白粉病诱导表达，呈双峰模式。为进一步研究中国野生毛葡萄株系‘丹凤-2’芪合成酶基因家族的表达及功能分析提供了基础。
3．在欧洲葡萄胚状体再生体系中，‘红地球’花药的最佳诱导培养基为NN69+CH0.5g·L~(-1)+PVP1g·L~(-1)＋2,4-D0.5mg·L~(-1)+BAP4.0mg·L~(-1)；子房的最佳诱导培养基为NN69+CH0.5g·L~(-1)+PVP1g·L~(-1)＋2,4-D1.0mg·L~(-1)+BAP4.0mg·L~(-1)。赤霞珠的花器官胚性愈伤组织的诱导效率要明显高于无核白。NN69培养基的诱导率最高（花药29.3%，子房32.5%），其次是B5培养基（花药4.5%，子房9.1%），MS培养基诱导率最低（花药4.0%，子房6.3%）。细胞悬浮培养的方法可迅速扩繁胚性愈伤组织，且能够获得均一的体细胞胚。
4．农杆菌介导的遗传转化中，潮霉素对愈伤组织的最低致死浓度为13mg·L~(-1)；采用此浓度对胚性愈伤组织进行抗性筛选。GV3101菌株转化率明显高于LBA4404菌株。转化后抗性愈伤组织随着继代次数的增加，报告基因GFP的表达逐渐增强。
The grapevine is one of the most important fruit crops in the world, which are generallysusceptible to Erysiphe necator and Plasmopara viticola. Stilbene synthase (STS) is the keyenzyme for producing the resveratrol. Grape resveratrol is a phytoalexin, plays an importantrole in plant defense responses to pathogens, and yet also shows significant health-promotingeffects including anti-cancer, prevention of cardiovascular disease and neuroprotectiveproperties. In this study, stilbene synthase genes were cloned from Chinese wild V.pseudoreticulata accession ‘Baihe-35-1’ which showed high resistance to powdery mildewand downy mildew. A stilbene synthase gene was clone from Chinese wild V. quinquangularisaccession ‘Danfeng-2’ which showed high resistance to powdery mildew and high content ofresveratrol in mature berries; Bioinformatics analysis and the study of transformation wereperformed in this study. The main results are described as followings.
1．STS sequences covered all coding region and3’ UTR were cloned from E. necatorinoculated-leaves of Chinese wild V. pseudoreticulata accession ‘Baihe-35-1’ by RACEamplification. RT-PCR products were analyzed by gel electrophoresis, cloned and more than800individual clones were sequenced. Sequence analysis identified that ‘Baihe-35-1’ have53single STS genes, four of which were pseudogene which do not encode stilbene synthasecorrectly. GenBank Accession numbers of STS from V. pseudoreticulata were from JQ886584to JQ886636. STS sequences from V. vinifera and V. pseudoreticulata sequences werehomologies, which the highest level can reach98%, but most of them cannot be grouped in asingle cluster.
2．VqDSTS1, was isolated by reverse transcription-polymerase chain reaction (RT-PCR)from Chinese wild Vitis quinquangularis accession 'Danfeng-2', and then analysed thesequence and expression model. The results suggested the full length cDNA sequence ofVqDSTS1was1179bp, and the accession number of VqDSTS1was JQ342086, and encoded392amino acid residues. Detailed analysis of the amino acid sequence revealed thatVqDSTS1contains "IPNSAGAIAGN" and "GVLFGFGPGLT" which can be found in allstilbene synthase gene family. With alignment of amino acid sequences, VqDSTS1shared highly identity between95.2%~98.7%with the stilbene synthase from other Vitis. From theanalysis of semi-quantitative, it was found that the expression model of VqDSTS1hadtwin-peak model. This work would make a foundation to investigate the function of stilbenesynthase gene family from Chinese wild Vitis quinquangularis accession 'Danfeng-2'.
3．During the construct process of the system of somatic embryos from Vitis vinifera, itwas found that the best induction medium was NN69+CH0.5g·L~(-1)+PVP1g·L~(-1)＋2,4-D0.5mg·L~(-1)+BAP4.0mg·L~(-1)from anthers of ‘Red Globe’; for the overies of ‘Red Globe’,NN69+CH0.5g·L~(-1)+PVP1g·L~(-1)＋2,4-D1.0mg·L~(-1)+BAP4.0mg·L~(-1). The embryo callusinduction rate from ‘Cabernet Sauvignon’ was obviously higher than the from ‘ThompsonSeedless’. The induction rate of NN69basic medium was the highest (anthers29.3%, overies32.5%); B5for the second (anthers4.5%, overies9.1%); MS the lowest (anthers4.0%, overies6.3%). The method of cell suspension culture could quickly proliferate the embryo callus cells,and then get the same somatic embryos.
4．During the agrobacterium-mediated transformation, the lethal concentration of Hygwas mg·L~(-1), efficiently when using this concentration of Hyg in the process of selecting theresistant callus. The tansformation rate of GV3101was obviously higher than LBA4404. Theexpression of report gene GFP was ever-increasing, when the resistant callus was sub-culturedfor several times.
引文
陈力耕,刘淑芳,胡西琴.2001.葡萄高效再生体系的建立及转LEAFY基因的研究.浙江大学学报(农业与生命科学版),27(5):523-526
段朝辉.2002.中国葡萄野生种白藜芦醇含量分析的研究[硕士学位论文].杨凌:西北农林科技大学
樊超红.2006.中国野生葡萄芪合酶基因转化无核葡萄的研究[硕士学位论文].杨凌:西北农林科技大学
方莉.2006.中国野生葡萄醛脱氢酶基因转化欧洲无核葡萄的研究[硕士学位论文].杨凌:西北农林科技大学
关心.2010.中国野生华东葡萄新基因乙二醛氧化酶基因遗传转化部分葡萄的研究[硕士学位论文].杨凌:西北农林科技大学
贺普超,王国英.1986.我国葡萄属野生种霜霉病抗性的调整研究.园艺学报,13(2):17-24
李浚明,植物组织培养教程[M],第二版.北京:中国农业大学出版社,2000:59-65
李云,冯慧,田砚亭.2002.“红地球”葡萄叶片、叶柄不定芽再生体系的建立.园艺学报,29(1):60-62
孙仲序,陈受宜,王建设.2003.农杆菌介导BADH基因转化葡萄研究.果树学报,20（2）:89-92
陶建敏,庄智敏,章镇,蔡斌华.2003.葡萄基因转导研究进展.果树学报,20(5):384-387
文颖强.2011.中国野生华东葡萄新基因VpALDH2a功能分析与分子机理研究[博士学位论文].杨凌:西北农林科技大学
王西平.2004.中国葡萄属野生种抗白粉病基因克隆与序列分析.[博士学位论文].杨凌:西北农林科技大学
王西平，刘斌，王跃进.毛葡萄芪合成酶基因的克隆及序列分析[J].西北植物学报,2007,27(8):1544–1549.
王跃进,贺普超.1997.中国葡萄属野生种叶片抗白粉病遗传研究.中国农业科学,30(1):19-25
徐伟荣.2005.中国葡萄属野生种芪合成酶基因载体构建及转化烟草研究[硕士学位论文].杨凌:西北农林科技大学
徐伟荣.2010.中国野葡萄抗白粉病芪合成酶基因启动子克隆与功能分析[博士学位论文].杨凌:西北农林科技大学
张剑侠,王跃进,李沛玲,张今今,潘学军,徐炎,唐海波.2004.中国野生葡萄的离体培养与快速繁殖.园艺学报,31(1):90-93
张克忠,鲍雪珍,自永延,颜望明,唐惕,曹湘玲,毛慧珠.1997.苏云金杆菌内毒素蛋自转入葡萄胚性愈伤组织细胞及转基因植株再生的研究.实验生物学报,30(3):303-309
赵素平，文志丰，侯鸿敏，肖欢，王跃进，王西平.2010.白粉菌诱导的中国野生毛葡萄抑制消减文库构建及EST序列分析.西北农林科技大学学报(自然科学版)，38（11）:109-113
支玉玺.2009.中国野生葡萄乙二醛氧化酶基因转化葡萄的研究[硕士学位论文].杨凌:西北农林科技大学
周鹏,王跃进,贺普超.2002.人胰岛素样生长因子-I基因转化葡萄的研究.热带作物报,23(1):54-61
Bais AJ, Murphy PJ and Dry IB.2000. The molecular regulation of stilbene phytoalexin biosynthesis inVitis vinifera during grape berry development. Functional Plant Biology,27(5):425-433
Bavaresco L, Fregoni C, Macedo Basto Gon alves MIVZ and Vezzulli S,2009. Physiology&MolecularBiology of Grapevine Stilbenes: An Update Grapevine Molecular Physiology&Biotechnology. In:Roubelakis-Angelakis K. A., ed.: Springer Netherlands,341-364.Borie B, Jeandet P, Parize A, BessisR and Adrian M,2004. Resveratrol and Stilbene Synthase mRNA Production in Grapevine LeavesTreated with Biotic and Abiotic Phytoalexin Elicitors. American Journal of Enology and Viticulture,55(1),60-64.
Bolwell G P, Wojtaszek P.1997.Mechanisms for the generation of reactive oxygen species in plant defense–a broad perspective. Physiol. Mol.Plant Pathol.51:347–366
Borie B, Jeandet P, Parize A, Bessis R and Adrian M.2004. Resveratrol and Stilbene Synthase mRNAProduction in Grapevine Leaves Treated with Biotic and Abiotic Phytoalexin Elicitors. AmericanJournal of Enology and Viticulture,55(1):60-64
Cadle-Davidson L,2008. Variation Within and Between Vitis spp. for Foliar Resistance to the DownyMildew Pathogen Plasmopara viticola. Plant Disease,92(11),1577-1584.
Chang X, Heene E, Qiao F and Nick P,2011. The Phytoalexin Resveratrol Regulates the Initiation ofHypersensitive Cell Death in Vitis Cell. PLoS ONE,6(10), e26405.
Cheng Z M, Reisch B I.1988. Shoot regeneration from Vitis labruscanaבCataeba’.Plant Cell Rep,8:403-406
Colby S M, Juncosa A M, Meredith C P.1991. Differences in Agrobacterium susceptibility andregenerative capacity restrict the development of transgenic grapevines. J.Amer Soc Hort Sci,116(2):356-361
Conforti F and Menichini F.2011. Phenolic Compounds from Plants as Nitric Oxide Production Inhibitors.Current Medicinal Chemistry,18(8):1137-1145
Coutos-Thévenot P, Poinssot B, Bonomelli A, Yean H, Breda C, Buffard D, Esnault R, Hain R and BoulayM.2001. In vitro tolerance to Botrytis cinerea of grapevine41B rootstock in transgenic plantsexpressing the stilbene synthase Vst1gene under the control of a pathogen-inducible PR10promoter.Journal of Experimental Botany,52(358):901-910
Das D K, Reddy M K, Upadhyaya K C, Sopory S K.2002.An efficient leaf-disc culture method for theregeneration via somatic embryogenesis and transformation of grape (Vitis vinifera L.). Plant CellReport,20:999–1005
Dhekney S A, Li Z T, Dutt M, Gray D J.2008. Agrobacterium-mediated transformation of embryogeniccultures and plant regeneration in Vitis rotundifolia Michx.(muscadine grape). Plant Cell Report,27:865–872
Dhekney S A, Li Z T.2009.Optimizing Initiation and Maintenance of Vitis Embryogenic Cultures.Hortscience,44(5):1400–1406
Dutt M, Li Z T, Dhekney S A, Gray D J.2007. Transgenic plants from shoot apical meristems of Vitisvinifera L.‘‘Thompson Seedless’’ via Agrobacterium-mediated transformation. Plant Cell Rep,26:2101–2110
Fan C, Pu N, Wang X, Wan Y, Fang L, Xu W, Zhang J.2008. Agrobacterium-mediated genetictransformation of grapevine (Vitis vinifera L.) with a novel stilbene synthase gene from Chinese wildVitis pseudoreticulata.Plant Cell Tiss Organ Cult,(92):197–206
Fernandez V, Videla L A.1996.Biochemical aspects of cellular antioxidant systems. Biol.Res.,29:177–182
Ferrer J L, Jez J M, Bowman M E, et al. Structure of chalcone synthase and the molecular basis of plantpolyketide biosynthesis [J]. Nature structural biology,1999,6(8),775–784.
Fettig S, Hess D.1999. Expression of a chimeric stilbene synthase gene in transgenic wheat lines.Transgenic Res.8:179-189.
Fischer R, Budde I and Hain R.1997. Stilbene synthase gene expression causes changes in flower colourand male sterility in tobacco. The Plant Journal,11(3):489-498
Franks T, He D G.1998. Regeneration of transgenic Vitis vinifera L. Sultana plants: genotypic andphenotypic analysis. Mol. Breed.,4:321-333
Mattivi F, Vrhovsek U, Malacarne G, Masuero D, Zulini L, Stefanini M, Moser C, Velasco R, Guella G.Profiling of Resveratrol Oligomers, Important Stress Metabolites, Accumulating in the Leaves ofHybrid Vitis vinifera (Merzling×Teroldego) Genotypes Infected with Plasmopara viticola [J]. Journalof Agricultural and Food Chemistry,2011,59(10):5364–5375.Gall L O, Torregrosa L, Danglot Y.1994.Agrobacterium-mediated genetic transformation of grapevine somatic embryos and regeneration oftransgenic plants expressing the coat protein of grapevine chrome mosaic nepovirus (GCMV). PlantSci.,102:161-170
Gindro K, Alonso-Villaverde V, Viret O, Spring J-L, Marti G, Wolfender J-L and Pezet R.2012. Stilbenes:Biomarkers of Grapevine Resistance to Disease of High Relevance for Agronomy, Oenology andHuman Health Plant Defence: Biological Control. In: Mérillon J. M. M. and Ramawat K. G. G., eds.:Springer Netherlands,25-54.(Progress in Biological Control; vol.12.)
Giorcelli A, Sparvoli F, Mattivi F, Tava A, Balestrazzi A, Vrhovsek U, Calligari P, Bollini R andConfalonieri M.2004. Expression of the stilbene synthase (StSy) gene from grapevine in transgenicwhite poplar results in high accumulation of the antioxidant resveratrol glucosides. Transgenic Res,13(3):203-214
Giorgio Gambino, Paola Ruffa, Rosalina Vallania, Ivana Gribaudo.2007. Somatic embryogenesis fromwhole flowers, anthers and ovaries of grapevine (Vitis spp.).Plant Cell Tiss Organ Cult,90:79–83
Goodwin PH, Hsiang T and Erickson L.2000. A comparison of stilbene and chalcone synthases including anew stilbene synthase gene from Vitis riparia cv. Gloire de Montpellier. Plant Science,151(1):1-8
Gray D. J.1989. Effects of dehydration and exogenous growth regulators on dormancy, quiescence andgermination of grape somatic embryos. In Vitro Cellular and Developmental Biology,25:1173-1178
Gray D. J.1992. Somatic embryogenesis and plant regeneration from immature zygotic embryos ofMuscadine grape (Vitis rotundifolia) cultivars. American Journal of Botany,79(5):542-546
Guan X, Zhao H, Xu Y and Wang Y.2011. Transient expression of glyoxal oxidase from the Chinese wildgrape Vitis pseudoreticulata can suppress powdery mildew in a susceptible genotype. Protoplasma,248(2):415-423
Hain, R., Bieseler, B., Kindl, H., Schr der, G. and St cker, R..1990. Expression of a stilbene synthase genein Nicotiana tabacum results in synthesis of the phytoalexin resveratrol. Plant Mol. Biol.15:325-335.
Hain R, Reif HJ, Krause E, Langebartels R, Kindl H, Vornam B, Wiese W, Schmelzer E, Schreier PH,Stocker RH and Et Al.1993. Disease resistance results from foreign phytoalexin expression in a novelplant. Nature,361(6408):153-156
Hebert D, Kikkert J R, Smith F S.1993.Optimization of biolistic transformation of embryogenic grape cellsuspension.Plant Cell Rep.,12:585-589
Hipskind JD and Paiva NL.2000. Constitutive accumulation of a resveratrol-glucoside in transgenic alfalfaincreases resistance to Phoma medicaginis. Mol Plant Microbe Interact,13(5):551-562
Holsters, M., Waele, D., Depicker, A. Transfecion and Transformation of Agrobacterium tumefaciens [J].Molec. Gen. Genet.1978,181-187.
Ingrosso I, Bonsegna S, De Domenico S, Laddomada B, Blando F, Santino A and Giovinazzo G.2011.Over-expression of a grape stilbene synthase gene in tomato induces parthenocarpy and causesabnormal pollen development. Plant Physiol Biochem,49(10):1092-1099
Iocco P, Franks T, Thomas M R,2001. Genetic transformation of major wine grape cultivars of Vitisvinifera L. Transg. Res.10:105–112
Jaillon O, Aury JM, Noel B, Policriti A, Clepet C, Casagrande A, Choisne N, Aubourg S, Vitulo N, Jubin C,Vezzi A, Legeai F, Hugueney P, Dasilva C, Horner D, Mica E, Jublot D, Poulain J, Bruyere C, BillaultA, Segurens B, Gouyvenoux M, Ugarte E, Cattonaro F, Anthouard V, Vico V, Del Fabbro C, Alaux M,Di Gaspero G, Dumas V, Felice N, Paillard S, Juman I, Moroldo M, Scalabrin S, Canaguier A, LeClainche I, Malacrida G, Durand E, Pesole G, Laucou V, Chatelet P, Merdinoglu D, Delledonne M,Pezzotti M, Lecharny A, Scarpelli C, Artiguenave F, Pe ME, Valle G, Morgante M, Caboche M,Adam-Blondon AF, Weissenbach J, Quetier F and Wincker P.2007. The grapevine genome sequencesuggests ancestral hexaploidization in major angiosperm phyla. Nature,449(7161),463-U465
Jang M, Cai L, Udeani GO, Slowing KV, Thomas CF, Beecher CW, Fong HH, Farnsworth NR, KinghornAD, Mehta RG, Moon RC and Pezzuto JM.1997. Cancer chemopreventive activity of resveratrol, anatural product derived from grapes. Science,275(5297):218-220
Khandelwal AR, Hebert VY and Dugas TR.2010. Essential role of ER-alpha-dependent NO production inresveratrol-mediated inhibition of restenosis. American Journal of Physiology-Heart and CirculatoryPhysiology,299(5): H1451-H1458
Kikkert J R, Ali G S, Wallace P G.2000.Expression of a fungal chitinase in Vitis vinifera L.‘Merlot’ and‘Chardonnay’ plants produced by biolistic transformation. Acta Horticulture,528:297-303
Kikkert J R, Hébert-Soulé D, Wallace P G,.1996.Transgenic plantlets of ‘Chancellor’ grapevine (Vitis sp.)from biolistic transformation of embryogenic cell suspensions. Plant Cell Rep.,15:311-316
Kobayashi S, Ding CK and Nakamura Y.2000. Kiwifruit (Actinidia deliciosa) transformed with a Vitisstilbene synthase gene produce piceid (resveratrol-glucoside). Plant Cell Rep,19:904-910
Kortekamp A.2006. Expression analysis of defence-related genes in grapevine leaves after inoculation witha host and a non-host pathogen. Plant Physiology and Biochemistry,44(1):58-67
Krastanova S, Perrin M, Barbier P, Demangeat G, Cornuet P, Bardonnet N, Otten L, Pinck L, Walter B.Transformation of grapevine rootstocks with the coat protein gene of grapevine fanleaf nepovirus[J].Plant Cell Rep,1995,14:550-554
Leckband G and L rz H.1998. Transformation and expression of a stilbene synthase gene of Vitis viniferaL. in barley and wheat for increased fungal resistance. TAG Theoretical and Applied Genetics,96(8):1004-1012
Liang H, Zheng J, Shuange JIA, Wang D, Ouyang J, Li J, Li L, Tian W, Jia X, Duan X, Sheng B, Hain R.2000. A transgenic wheat with a stilbene synthase gene resistant to powdery mildew obtained bybiolistic method. Chin. Sci. Bull.45:634-638.
Liu X J, Huang B B, Lin J, Fei J, Chen Z H, Pang Y Z, Sun X F and Tang K X.2006. A novelpathogenesis-related protein (SsPR10) from Solanum surattense with ribonucleolytic andantimicrobial activity is stress-and pathogen-inducible. Journal of Plant Physiology,163(5):546-556
Li Z T, Dhekney S A, Dutt M,Gray D J.2008.An improved protocol for Agrobacterium-mediatedtransformation of grapevine (Vitis vinifera L.) Plant Cell Tiss Organ Cult,93:311–321
Li Z T, Dhekney S, Dutt M, Aman V M, Tattersall J, Kelley K T,Gray D J.2006. OptimizingAgrobacterium-mediated transformation of grapevine. In Vitro Cellular&Developmental Biology-Plant.42(3):220-227
Liswidowati F, M. F., Hohmann F, Schwer B,and Kindl H.1991. Induction of stilbene synthase by Botrytiscinerea in cultured grapevine cells. Planta183:307-314
Lu X, Ma L, Ruan L, Kong Y, Mou H, Zhang Z, Wang Z, Wang JM and Le Y.2010. Resveratroldifferentially modulates inflammatory responses of microglia and astrocytes. Journal ofNeuroinflammation,7(1):46
Mauro M C, Toutain S, Walter B.1995. High efficiency regeneration of grapevine plants transferred withthe GFLV coat protein gene. Plant Sci.,112:97-106
Melchior F and Kindl H.1991. Coordinate-and elicitor-dependent expression of stilbene synthase andphenylalanine ammonia-lyase genes in Vitis cv. Optima. Arch Biochem Biophys,288(2):552-557
Mullins M G, Srinivasan C.1976.Somatic embryos and plantlets from an ancient clone of the grapevine(cv.Cabernet Sauvignon) by apomixes in vitro. Journal of Experimental Botany,27:1022-1030
Patrice T, Thierry L, Mark R T. Historical origins and genetic diversityof wine grapes[J]. Trends inGenetics.2006,22(9):5112519
Popescu C F.1996. Somatic embryogenesis and plant development from anther culture of Vitis vinifera L..Plant Growth Reg,20:75-78
Richter A, de Kathen A, de Lorenzo G, Briviba K, Hain R, Ramsay G, Jacobsen HJ, Kiesecker H.2006.Transgenic peas (Pisum sativum) expressing polygalacturonase inhibiting protein from raspberry(Rubus idaeus) and stilbene synthase from grape (Vitis vinifera). Plant Cell Rep.25:1166-1173
Richter H, Pezet R, Viret O and Gindro K.2005. Characterization of3new partial stilbene synthase genesout of over20expressed in Vitis vinifera during the interaction with Plasmopara viticola.Physiological and Molecular Plant Pathology,67(3-5),248-260
Ruhmann S, Treutter D, Fritsche S, Briviba K and Szankowski I.2006. Piceid (resveratrol glucoside)synthesis in stilbene synthase transgenic apple fruit. J Agric Food Chem,54(13):4633-4640
Rupprich, N, Kindl, H.1978. Stilbene synthases and stilbenecarboxylatesynthases: I.Enzymatics ynthesis of3,5’-4-trihydroxy stilbene from p-coumaroyl coenzyme A and malonyl coenzymeA. Hoppe Seylers ZPhysiol Chem,359:165-172
Schnee S, Viret O and Gindro K.2008. Role of stilbenes in the resistance of grapevine to powdery mildew.Physiological and Molecular Plant Pathology,72(4–6):128-133
Schroder G, Brown JW and Schroder J.1988. Molecular analysis of resveratrol synthase. cDNA, genomicclones and relationship with chalcone synthase. Eur J Biochem,172(1):161-169
Scorza R. and Cordts J M.1996. Producing transgenic ‘Thompson Seedless’ grape (Vitis vinifera L.) plants.J Amer. Soc. Sci.,121(4):616-619
Serazetdinova L, Oldach KH and Lorz H.2005. Expression of transgenic stilbene synthases in wheatcauses the accumulation of unknown stilbene derivatives with antifungal activity. J Plant Physiol,162(9):985-1002
Sparvoli F, Martin C, Scienza A, Gavazzi G and Tonelli C.1994. Cloning and molecular analysis ofstructural genes involved in flavonoid and stilbene biosynthesis in grape (Vitis vinifera L.). Plant MolBiol,24(5):743-755
Stark-Lorenzen P, Nelke B, H n ler G, Mühlbach HP and Thomzik JE.1997. Transfer of a grapevinestilbene synthase gene to rice (Oryza sativa L.). Plant Cell Reports,16(10):668-673
Szankowski I, Briviba K, Fleschhut J, Sch nherr J, Jacobsen HJ and Kiesecker H.2003. Transformation ofapple (Malus domestica Borkh.) with the stilbene synthase gene from grapevine (Vitis vinifera L.)and a PGIP gene from kiwi (Actinidia deliciosa). Plant Cell Reports,22(2):141-149
Thomzik JE, Stenzel K, St cker R, Schreier PH, Hain R and Stahl DJ.1997. Synthesis of a grapevinephytoalexin in transgenic tomatoes (Lycopersicon esculentum Mill.) conditions resistance againstPhytophthora infestans. Physiological and Molecular Plant Pathology,51(4):265-278
Tropf S, Lanz T, Rensing SA, Schr der J and Schr der G.1994. Evidence that stilbene synthases havedeveloped from chalcone synthases several times in the course of evolution. Journal of MolecularEvolution,38(6):610-618
Tsvtkov I J, Atanassov A V, Tsolova V M.2000. Gene transfer for stress resistance in grapes. Proceedingsof the seventh international symposium on grapevine genetics and breeding. Acta Horticulturae,528:389-394
Vergara D, Simeone P, Toraldo D, Del Boccio P, Vergaro V, Leporatti S, Pieragostino D, Tinelli A, DeDomenico S, Alberti S, Urbani A, Salzet M, Santino A and Maffia M.2012. Resveratroldownregulates Akt/GSK and ERK signalling pathways in OVCAR-3ovarian cancer cells. Mol Biosyst,(4):1078-1087
Velasco R, Zharkikh A, Troggio M, Cartwright DA, Cestaro A, Pruss D, Pindo M, Fitzgerald LM, VezzulliS, Reid J, Malacarne G, Iliev D, Coppola G, Wardell B, Micheletti D, Macalma T, Facci M, Mitchell JT,Perazzolli M, Eldredge G, Gatto P, Oyzerski R, Moretto M, Gutin N, Stefanini M, Chen Y, Segala C,Davenport C, Dematte L, Mraz A, Battilana J, Stormo K, Costa F, Tao QZ, Si-Ammour A, Harkins T,Lackey A, Perbost C, Taillon B, Stella A, Solovyev V, Fawcett JA, Sterck L, Vandepoele K, GrandoSM, Toppo S, Moser C, Lanchbury J, Bogden R, Skolnick M, Sgaramella V, Bhatnagar SK, Fontana P,Gutin A, Van De Peer Y, Salamini F and Viola R.2007. A High Quality Draft Consensus Sequence ofthe Genome of a Heterozygous Grapevine Variety. PLoS ONE,2(12): e1326
Versari A, Parpinello GP, Tornielli GB, Ferrarini R and Giulivo C.2001. Stilbene compounds and stilbenesynthase expression during ripening, wilting, and UV treatment in grape cv. Corvina. J Agric FoodChem,49(11):5531-5536
Vezzulli S, Battilani P and Bavaresco L.2007a. Stilbene-Synthase Gene Expression after Aspergilluscarbonarius Infection in Grapes. American Journal of Enology and Viticulture,58(1):132-134
Vezzulli S, Civardi S, Ferrari F and Bavaresco L.2007b. Methyl Jasmonate Treatment as a Trigger ofResveratrol Synthesis in Cultivated Grapevine. American Journal of Enology and Viticulture,58(4):530-533
Wan Y, Schwaninger H, He P and Wang Y.2007. Comparison of resistance to powdery mildew and downymildew in Chinese wild grapes. Vitis,46(3):132-136
Wang Q C, Mawassi M, Sahar N, Li P, Violeta C, Gafny R, Sela I, Tanne E1, Perl A.2004.Cryopreservationof grapevine (Vitis spp.) embryogenic cell suspensions by encapsulation–vitrification. Plant Cell,Tissue and Organ Culture77:267–275
Wang X P, Wang Y J, Hao W. cDNA cloning and characterization of the novel genes related to aldehydedehydrogenase from wild Chinese grape (Vitis pseudoreticulata W. T. Wang)[J]. DNA Sequence,2007,18(1):9–18.
Wang Y, Liu Y, He P, Chen J, Lamikanra O and Lu J.1995. Evaluation of foliar resistance to Uncinulanecator in Chinese wild Vitis species. Vitis,34(3):159-164
Wiese W, Vornam B, Krause E and Kindl H.1994. Structural organization and differential expression ofthree stilbene synthase genes located on a13kb grapevine DNA fragment. Plant Mol Biol,26(2),667-677
Xu W, Yu Y, Ding J, Hua Z and Wang Y.2010a. Characterization of a novel stilbene synthase promoterinvolved in pathogen-and stress-inducible expression from Chinese wild Vitis pseudoreticulata. Planta,231(2):475-487
Xu Y, Zhu Z, Xiao Y and Wang Y.2009. Construction of a cDNA Library of Vitis pseudoreticulata Nativeto China inoculated with Uncinula necator and the analysis of potential defence-related expressedsequence Tags (ESTs). South African Society for Enology&Viticulture,30(1):65-71
Xu WR, Yu YH, Zhou Q, Ding JH, Dai LM, Xie XQ, Xu Y, Zhang CH and Wang YJ.2011. Expressionpattern, genomic structure, and promoter analysis of the gene encoding stilbene synthase from Chinesewild Vitis pseudoreticulata. Journal of Experimental Botany,62(8):2745-2761
Xue B, Ling K S, Reid C L.1999.Transformation of five grape rootstocks with plant virus genes and avirE2gene from Agrobacteriu tumefaciens. In Vitro Cellular and Developmental Biology Plant,35(3):226-231
Yamamoto T, Iketani H, Ieki H, Nishizawa Y, Notsuka K, Hibi T, Hayashi T, Matsuta N.2000.Transgenicgrapevine plants expressing a rice chitinase with enhanced resistance to fungal pathogens. Plant CellRep,19:639-646
Yu CKY, Springob K, Schmidt JR, Nicholson RL, Chu IK, Yip WK and Lo C.2005. A stilbene synthasegene (SbSTS1) is involved in host and nonhost defense responses in Sorghum. Plant Physiology,138(1):393-401
Zhang J, Wang Y, Wang X, Yang K and Yang J.2003. An Improved Method for Rapidly Extracting TotalRNA from Vitis. Journal of Fruit Science,20(3):178-181
Zhang J, Wang Y and Xu Y.2002. The Resistance of Chinese Wild Vitis to Uncinula necator and itsInheritance in F1Generation. Agricultural Sciences in China,1(2):195-199
段朝辉.2002.中国葡萄野生种白藜芦醇含量分析的研究[硕士学位论文].杨凌:西北农林科技大学
樊超红.2006.中国野生葡萄芪合酶基因转化无核葡萄的研究[硕士学位论文].杨凌:西北农林科技大学
方莉.2006.中国野生葡萄醛脱氢酶基因转化欧洲无核葡萄的研究[硕士学位论文].杨凌:西北农林科技大学
关心.2010.中国野生华东葡萄新基因乙二醛氧化酶基因遗传转化部分葡萄的研究[硕士学位论文].杨凌:西北农林科技大学
贺普超,王国英.1986.我国葡萄属野生种霜霉病抗性的调整研究.园艺学报,13(2):17-24
李浚明,植物组织培养教程[M],第二版.北京:中国农业大学出版社,2000:59-65
李云,冯慧,田砚亭.2002.“红地球”葡萄叶片、叶柄不定芽再生体系的建立.园艺学报,29(1):60-62
孙仲序,陈受宜,王建设.2003.农杆菌介导BADH基因转化葡萄研究.果树学报,20（2）:89-92
陶建敏,庄智敏,章镇,蔡斌华.2003.葡萄基因转导研究进展.果树学报,20(5):384-387
文颖强.2011.中国野生华东葡萄新基因VpALDH2a功能分析与分子机理研究[博士学位论文].杨凌:西北农林科技大学
王西平.2004.中国葡萄属野生种抗白粉病基因克隆与序列分析.[博士学位论文].杨凌:西北农林科技大学
王西平，刘斌，王跃进.毛葡萄芪合成酶基因的克隆及序列分析[J].西北植物学报,2007,27(8):1544–1549.
王跃进,贺普超.1997.中国葡萄属野生种叶片抗白粉病遗传研究.中国农业科学,30(1):19-25
徐伟荣.2005.中国葡萄属野生种芪合成酶基因载体构建及转化烟草研究[硕士学位论文].杨凌:西北农林科技大学
徐伟荣.2010.中国野葡萄抗白粉病芪合成酶基因启动子克隆与功能分析[博士学位论文].杨凌:西北农林科技大学
张剑侠,王跃进,李沛玲,张今今,潘学军,徐炎,唐海波.2004.中国野生葡萄的离体培养与快速繁殖.园艺学报,31(1):90-93
张克忠,鲍雪珍,自永延,颜望明,唐惕,曹湘玲,毛慧珠.1997.苏云金杆菌内毒素蛋自转入葡萄胚性愈伤组织细胞及转基因植株再生的研究.实验生物学报,30(3):303-309
赵素平，文志丰，侯鸿敏，肖欢，王跃进，王西平.2010.白粉菌诱导的中国野生毛葡萄抑制消减文库构建及EST序列分析.西北农林科技大学学报(自然科学版)，38（11）:109-113
支玉玺.2009.中国野生葡萄乙二醛氧化酶基因转化葡萄的研究[硕士学位论文].杨凌:西北农林科技大学
周鹏,王跃进,贺普超.2002.人胰岛素样生长因子-I基因转化葡萄的研究.热带作物报,23(1):54-61
Bais AJ, Murphy PJ and Dry IB.2000. The molecular regulation of stilbene phytoalexin biosynthesis inVitis vinifera during grape berry development. Functional Plant Biology,27(5):425-433
Bavaresco L, Fregoni C, Macedo Basto Gon alves MIVZ and Vezzulli S,2009. Physiology&MolecularBiology of Grapevine Stilbenes: An Update Grapevine Molecular Physiology&Biotechnology. In:Roubelakis-Angelakis K. A., ed.: Springer Netherlands,341-364.Borie B, Jeandet P, Parize A, BessisR and Adrian M,2004. Resveratrol and Stilbene Synthase mRNA Production in Grapevine LeavesTreated with Biotic and Abiotic Phytoalexin Elicitors. American Journal of Enology and Viticulture,55(1),60-64.
Bolwell G P, Wojtaszek P.1997.Mechanisms for the generation of reactive oxygen species in plant defense–a broad perspective. Physiol. Mol.Plant Pathol.51:347–366
Borie B, Jeandet P, Parize A, Bessis R and Adrian M.2004. Resveratrol and Stilbene Synthase mRNAProduction in Grapevine Leaves Treated with Biotic and Abiotic Phytoalexin Elicitors. AmericanJournal of Enology and Viticulture,55(1):60-64
Cadle-Davidson L,2008. Variation Within and Between Vitis spp. for Foliar Resistance to the DownyMildew Pathogen Plasmopara viticola. Plant Disease,92(11),1577-1584.
Chang X, Heene E, Qiao F and Nick P,2011. The Phytoalexin Resveratrol Regulates the Initiation ofHypersensitive Cell Death in Vitis Cell. PLoS ONE,6(10), e26405.
Cheng Z M, Reisch B I.1988. Shoot regeneration from Vitis labruscanaבCataeba’.Plant Cell Rep,8:403-406
Colby S M, Juncosa A M, Meredith C P.1991. Differences in Agrobacterium susceptibility andregenerative capacity restrict the development of transgenic grapevines. J.Amer Soc Hort Sci,116(2):356-361
Conforti F and Menichini F.2011. Phenolic Compounds from Plants as Nitric Oxide Production Inhibitors.Current Medicinal Chemistry,18(8):1137-1145
Coutos-Thévenot P, Poinssot B, Bonomelli A, Yean H, Breda C, Buffard D, Esnault R, Hain R and BoulayM.2001. In vitro tolerance to Botrytis cinerea of grapevine41B rootstock in transgenic plantsexpressing the stilbene synthase Vst1gene under the control of a pathogen-inducible PR10promoter.Journal of Experimental Botany,52(358):901-910
Das D K, Reddy M K, Upadhyaya K C, Sopory S K.2002.An efficient leaf-disc culture method for theregeneration via somatic embryogenesis and transformation of grape (Vitis vinifera L.). Plant CellReport,20:999–1005
Dhekney S A, Li Z T, Dutt M, Gray D J.2008. Agrobacterium-mediated transformation of embryogeniccultures and plant regeneration in Vitis rotundifolia Michx.(muscadine grape). Plant Cell Report,27:865–872
Dhekney S A, Li Z T.2009.Optimizing Initiation and Maintenance of Vitis Embryogenic Cultures.Hortscience,44(5):1400–1406
Dutt M, Li Z T, Dhekney S A, Gray D J.2007. Transgenic plants from shoot apical meristems of Vitisvinifera L.‘‘Thompson Seedless’’ via Agrobacterium-mediated transformation. Plant Cell Rep,26:2101–2110
Fan C, Pu N, Wang X, Wan Y, Fang L, Xu W, Zhang J.2008. Agrobacterium-mediated genetictransformation of grapevine (Vitis vinifera L.) with a novel stilbene synthase gene from Chinese wildVitis pseudoreticulata.Plant Cell Tiss Organ Cult,(92):197–206
Fernandez V, Videla L A.1996.Biochemical aspects of cellular antioxidant systems. Biol.Res.,29:177–182
Ferrer J L, Jez J M, Bowman M E, et al. Structure of chalcone synthase and the molecular basis of plantpolyketide biosynthesis [J]. Nature structural biology,1999,6(8),775–784.
Fettig S, Hess D.1999. Expression of a chimeric stilbene synthase gene in transgenic wheat lines.Transgenic Res.8:179-189.
Fischer R, Budde I and Hain R.1997. Stilbene synthase gene expression causes changes in flower colourand male sterility in tobacco. The Plant Journal,11(3):489-498
Franks T, He D G.1998. Regeneration of transgenic Vitis vinifera L. Sultana plants: genotypic andphenotypic analysis. Mol. Breed.,4:321-333
Mattivi F, Vrhovsek U, Malacarne G, Masuero D, Zulini L, Stefanini M, Moser C, Velasco R, Guella G.Profiling of Resveratrol Oligomers, Important Stress Metabolites, Accumulating in the Leaves ofHybrid Vitis vinifera (Merzling×Teroldego) Genotypes Infected with Plasmopara viticola [J]. Journalof Agricultural and Food Chemistry,2011,59(10):5364–5375.Gall L O, Torregrosa L, Danglot Y.1994.Agrobacterium-mediated genetic transformation of grapevine somatic embryos and regeneration oftransgenic plants expressing the coat protein of grapevine chrome mosaic nepovirus (GCMV). PlantSci.,102:161-170
Gindro K, Alonso-Villaverde V, Viret O, Spring J-L, Marti G, Wolfender J-L and Pezet R.2012. Stilbenes:Biomarkers of Grapevine Resistance to Disease of High Relevance for Agronomy, Oenology andHuman Health Plant Defence: Biological Control. In: Mérillon J. M. M. and Ramawat K. G. G., eds.:Springer Netherlands,25-54.(Progress in Biological Control; vol.12.)
Giorcelli A, Sparvoli F, Mattivi F, Tava A, Balestrazzi A, Vrhovsek U, Calligari P, Bollini R andConfalonieri M.2004. Expression of the stilbene synthase (StSy) gene from grapevine in transgenicwhite poplar results in high accumulation of the antioxidant resveratrol glucosides. Transgenic Res,13(3):203-214
Giorgio Gambino, Paola Ruffa, Rosalina Vallania, Ivana Gribaudo.2007. Somatic embryogenesis fromwhole flowers, anthers and ovaries of grapevine (Vitis spp.).Plant Cell Tiss Organ Cult,90:79–83
Goodwin PH, Hsiang T and Erickson L.2000. A comparison of stilbene and chalcone synthases including anew stilbene synthase gene from Vitis riparia cv. Gloire de Montpellier. Plant Science,151(1):1-8
Gray D. J.1989. Effects of dehydration and exogenous growth regulators on dormancy, quiescence andgermination of grape somatic embryos. In Vitro Cellular and Developmental Biology,25:1173-1178
Gray D. J.1992. Somatic embryogenesis and plant regeneration from immature zygotic embryos ofMuscadine grape (Vitis rotundifolia) cultivars. American Journal of Botany,79(5):542-546
Guan X, Zhao H, Xu Y and Wang Y.2011. Transient expression of glyoxal oxidase from the Chinese wildgrape Vitis pseudoreticulata can suppress powdery mildew in a susceptible genotype. Protoplasma,248(2):415-423
Hain, R., Bieseler, B., Kindl, H., Schr der, G. and St cker, R..1990. Expression of a stilbene synthase genein Nicotiana tabacum results in synthesis of the phytoalexin resveratrol. Plant Mol. Biol.15:325-335.
Hain R, Reif HJ, Krause E, Langebartels R, Kindl H, Vornam B, Wiese W, Schmelzer E, Schreier PH,Stocker RH and Et Al.1993. Disease resistance results from foreign phytoalexin expression in a novelplant. Nature,361(6408):153-156
Hebert D, Kikkert J R, Smith F S.1993.Optimization of biolistic transformation of embryogenic grape cellsuspension.Plant Cell Rep.,12:585-589
Hipskind JD and Paiva NL.2000. Constitutive accumulation of a resveratrol-glucoside in transgenic alfalfaincreases resistance to Phoma medicaginis. Mol Plant Microbe Interact,13(5):551-562
Holsters, M., Waele, D., Depicker, A. Transfecion and Transformation of Agrobacterium tumefaciens [J].Molec. Gen. Genet.1978,181-187.
Ingrosso I, Bonsegna S, De Domenico S, Laddomada B, Blando F, Santino A and Giovinazzo G.2011.Over-expression of a grape stilbene synthase gene in tomato induces parthenocarpy and causesabnormal pollen development. Plant Physiol Biochem,49(10):1092-1099
Iocco P, Franks T, Thomas M R,2001. Genetic transformation of major wine grape cultivars of Vitisvinifera L. Transg. Res.10:105–112
Jaillon O, Aury JM, Noel B, Policriti A, Clepet C, Casagrande A, Choisne N, Aubourg S, Vitulo N, Jubin C,Vezzi A, Legeai F, Hugueney P, Dasilva C, Horner D, Mica E, Jublot D, Poulain J, Bruyere C, BillaultA, Segurens B, Gouyvenoux M, Ugarte E, Cattonaro F, Anthouard V, Vico V, Del Fabbro C, Alaux M,Di Gaspero G, Dumas V, Felice N, Paillard S, Juman I, Moroldo M, Scalabrin S, Canaguier A, LeClainche I, Malacrida G, Durand E, Pesole G, Laucou V, Chatelet P, Merdinoglu D, Delledonne M,Pezzotti M, Lecharny A, Scarpelli C, Artiguenave F, Pe ME, Valle G, Morgante M, Caboche M,Adam-Blondon AF, Weissenbach J, Quetier F and Wincker P.2007. The grapevine genome sequencesuggests ancestral hexaploidization in major angiosperm phyla. Nature,449(7161),463-U465
Jang M, Cai L, Udeani GO, Slowing KV, Thomas CF, Beecher CW, Fong HH, Farnsworth NR, KinghornAD, Mehta RG, Moon RC and Pezzuto JM.1997. Cancer chemopreventive activity of resveratrol, anatural product derived from grapes. Science,275(5297):218-220
Khandelwal AR, Hebert VY and Dugas TR.2010. Essential role of ER-alpha-dependent NO production inresveratrol-mediated inhibition of restenosis. American Journal of Physiology-Heart and CirculatoryPhysiology,299(5): H1451-H1458
Kikkert J R, Ali G S, Wallace P G.2000.Expression of a fungal chitinase in Vitis vinifera L.‘Merlot’ and‘Chardonnay’ plants produced by biolistic transformation. Acta Horticulture,528:297-303
Kikkert J R, Hébert-Soulé D, Wallace P G,.1996.Transgenic plantlets of ‘Chancellor’ grapevine (Vitis sp.)from biolistic transformation of embryogenic cell suspensions. Plant Cell Rep.,15:311-316
Kobayashi S, Ding CK and Nakamura Y.2000. Kiwifruit (Actinidia deliciosa) transformed with a Vitisstilbene synthase gene produce piceid (resveratrol-glucoside). Plant Cell Rep,19:904-910
Kortekamp A.2006. Expression analysis of defence-related genes in grapevine leaves after inoculation witha host and a non-host pathogen. Plant Physiology and Biochemistry,44(1):58-67
Krastanova S, Perrin M, Barbier P, Demangeat G, Cornuet P, Bardonnet N, Otten L, Pinck L, Walter B.Transformation of grapevine rootstocks with the coat protein gene of grapevine fanleaf nepovirus[J].Plant Cell Rep,1995,14:550-554
Leckband G and L rz H.1998. Transformation and expression of a stilbene synthase gene of Vitis viniferaL. in barley and wheat for increased fungal resistance. TAG Theoretical and Applied Genetics,96(8):1004-1012
Liang H, Zheng J, Shuange JIA, Wang D, Ouyang J, Li J, Li L, Tian W, Jia X, Duan X, Sheng B, Hain R.2000. A transgenic wheat with a stilbene synthase gene resistant to powdery mildew obtained bybiolistic method. Chin. Sci. Bull.45:634-638.
Liu X J, Huang B B, Lin J, Fei J, Chen Z H, Pang Y Z, Sun X F and Tang K X.2006. A novelpathogenesis-related protein (SsPR10) from Solanum surattense with ribonucleolytic andantimicrobial activity is stress-and pathogen-inducible. Journal of Plant Physiology,163(5):546-556
Li Z T, Dhekney S A, Dutt M,Gray D J.2008.An improved protocol for Agrobacterium-mediatedtransformation of grapevine (Vitis vinifera L.) Plant Cell Tiss Organ Cult,93:311–321
Li Z T, Dhekney S, Dutt M, Aman V M, Tattersall J, Kelley K T,Gray D J.2006. OptimizingAgrobacterium-mediated transformation of grapevine. In Vitro Cellular&Developmental Biology-Plant.42(3):220-227
Liswidowati F, M. F., Hohmann F, Schwer B,and Kindl H.1991. Induction of stilbene synthase by Botrytiscinerea in cultured grapevine cells. Planta183:307-314
Lu X, Ma L, Ruan L, Kong Y, Mou H, Zhang Z, Wang Z, Wang JM and Le Y.2010. Resveratroldifferentially modulates inflammatory responses of microglia and astrocytes. Journal ofNeuroinflammation,7(1):46
Mauro M C, Toutain S, Walter B.1995. High efficiency regeneration of grapevine plants transferred withthe GFLV coat protein gene. Plant Sci.,112:97-106
Melchior F and Kindl H.1991. Coordinate-and elicitor-dependent expression of stilbene synthase andphenylalanine ammonia-lyase genes in Vitis cv. Optima. Arch Biochem Biophys,288(2):552-557
Mullins M G, Srinivasan C.1976.Somatic embryos and plantlets from an ancient clone of the grapevine(cv.Cabernet Sauvignon) by apomixes in vitro. Journal of Experimental Botany,27:1022-1030
Patrice T, Thierry L, Mark R T. Historical origins and genetic diversityof wine grapes[J]. Trends inGenetics.2006,22(9):5112519
Popescu C F.1996. Somatic embryogenesis and plant development from anther culture of Vitis vinifera L..Plant Growth Reg,20:75-78
Richter A, de Kathen A, de Lorenzo G, Briviba K, Hain R, Ramsay G, Jacobsen HJ, Kiesecker H.2006.Transgenic peas (Pisum sativum) expressing polygalacturonase inhibiting protein from raspberry(Rubus idaeus) and stilbene synthase from grape (Vitis vinifera). Plant Cell Rep.25:1166-1173
Richter H, Pezet R, Viret O and Gindro K.2005. Characterization of3new partial stilbene synthase genesout of over20expressed in Vitis vinifera during the interaction with Plasmopara viticola.Physiological and Molecular Plant Pathology,67(3-5),248-260
Ruhmann S, Treutter D, Fritsche S, Briviba K and Szankowski I.2006. Piceid (resveratrol glucoside)synthesis in stilbene synthase transgenic apple fruit. J Agric Food Chem,54(13):4633-4640
Rupprich, N, Kindl, H.1978. Stilbene synthases and stilbenecarboxylatesynthases: I.Enzymatics ynthesis of3,5’-4-trihydroxy stilbene from p-coumaroyl coenzyme A and malonyl coenzymeA. Hoppe Seylers ZPhysiol Chem,359:165-172
Schnee S, Viret O and Gindro K.2008. Role of stilbenes in the resistance of grapevine to powdery mildew.Physiological and Molecular Plant Pathology,72(4–6):128-133
Schroder G, Brown JW and Schroder J.1988. Molecular analysis of resveratrol synthase. cDNA, genomicclones and relationship with chalcone synthase. Eur J Biochem,172(1):161-169
Scorza R. and Cordts J M.1996. Producing transgenic ‘Thompson Seedless’ grape (Vitis vinifera L.) plants.J Amer. Soc. Sci.,121(4):616-619
Serazetdinova L, Oldach KH and Lorz H.2005. Expression of transgenic stilbene synthases in wheatcauses the accumulation of unknown stilbene derivatives with antifungal activity. J Plant Physiol,162(9):985-1002
Sparvoli F, Martin C, Scienza A, Gavazzi G and Tonelli C.1994. Cloning and molecular analysis ofstructural genes involved in flavonoid and stilbene biosynthesis in grape (Vitis vinifera L.). Plant MolBiol,24(5):743-755
Stark-Lorenzen P, Nelke B, H n ler G, Mühlbach HP and Thomzik JE.1997. Transfer of a grapevinestilbene synthase gene to rice (Oryza sativa L.). Plant Cell Reports,16(10):668-673
Szankowski I, Briviba K, Fleschhut J, Sch nherr J, Jacobsen HJ and Kiesecker H.2003. Transformation ofapple (Malus domestica Borkh.) with the stilbene synthase gene from grapevine (Vitis vinifera L.)and a PGIP gene from kiwi (Actinidia deliciosa). Plant Cell Reports,22(2):141-149
Thomzik JE, Stenzel K, St cker R, Schreier PH, Hain R and Stahl DJ.1997. Synthesis of a grapevinephytoalexin in transgenic tomatoes (Lycopersicon esculentum Mill.) conditions resistance againstPhytophthora infestans. Physiological and Molecular Plant Pathology,51(4):265-278
Tropf S, Lanz T, Rensing SA, Schr der J and Schr der G.1994. Evidence that stilbene synthases havedeveloped from chalcone synthases several times in the course of evolution. Journal of MolecularEvolution,38(6):610-618
Tsvtkov I J, Atanassov A V, Tsolova V M.2000. Gene transfer for stress resistance in grapes. Proceedingsof the seventh international symposium on grapevine genetics and breeding. Acta Horticulturae,528:389-394
Vergara D, Simeone P, Toraldo D, Del Boccio P, Vergaro V, Leporatti S, Pieragostino D, Tinelli A, DeDomenico S, Alberti S, Urbani A, Salzet M, Santino A and Maffia M.2012. Resveratroldownregulates Akt/GSK and ERK signalling pathways in OVCAR-3ovarian cancer cells. Mol Biosyst,(4):1078-1087
Velasco R, Zharkikh A, Troggio M, Cartwright DA, Cestaro A, Pruss D, Pindo M, Fitzgerald LM, VezzulliS, Reid J, Malacarne G, Iliev D, Coppola G, Wardell B, Micheletti D, Macalma T, Facci M, Mitchell JT,Perazzolli M, Eldredge G, Gatto P, Oyzerski R, Moretto M, Gutin N, Stefanini M, Chen Y, Segala C,Davenport C, Dematte L, Mraz A, Battilana J, Stormo K, Costa F, Tao QZ, Si-Ammour A, Harkins T,Lackey A, Perbost C, Taillon B, Stella A, Solovyev V, Fawcett JA, Sterck L, Vandepoele K, GrandoSM, Toppo S, Moser C, Lanchbury J, Bogden R, Skolnick M, Sgaramella V, Bhatnagar SK, Fontana P,Gutin A, Van De Peer Y, Salamini F and Viola R.2007. A High Quality Draft Consensus Sequence ofthe Genome of a Heterozygous Grapevine Variety. PLoS ONE,2(12): e1326
Versari A, Parpinello GP, Tornielli GB, Ferrarini R and Giulivo C.2001. Stilbene compounds and stilbenesynthase expression during ripening, wilting, and UV treatment in grape cv. Corvina. J Agric FoodChem,49(11):5531-5536
Vezzulli S, Battilani P and Bavaresco L.2007a. Stilbene-Synthase Gene Expression after Aspergilluscarbonarius Infection in Grapes. American Journal of Enology and Viticulture,58(1):132-134
Vezzulli S, Civardi S, Ferrari F and Bavaresco L.2007b. Methyl Jasmonate Treatment as a Trigger ofResveratrol Synthesis in Cultivated Grapevine. American Journal of Enology and Viticulture,58(4):530-533
Wan Y, Schwaninger H, He P and Wang Y.2007. Comparison of resistance to powdery mildew and downymildew in Chinese wild grapes. Vitis,46(3):132-136
Wang Q C, Mawassi M, Sahar N, Li P, Violeta C, Gafny R, Sela I, Tanne E1, Perl A.2004.Cryopreservationof grapevine (Vitis spp.) embryogenic cell suspensions by encapsulation–vitrification. Plant Cell,Tissue and Organ Culture77:267–275
Wang X P, Wang Y J, Hao W. cDNA cloning and characterization of the novel genes related to aldehydedehydrogenase from wild Chinese grape (Vitis pseudoreticulata W. T. Wang)[J]. DNA Sequence,2007,18(1):9–18.
Wang Y, Liu Y, He P, Chen J, Lamikanra O and Lu J.1995. Evaluation of foliar resistance to Uncinulanecator in Chinese wild Vitis species. Vitis,34(3):159-164
Wiese W, Vornam B, Krause E and Kindl H.1994. Structural organization and differential expression ofthree stilbene synthase genes located on a13kb grapevine DNA fragment. Plant Mol Biol,26(2),667-677
Xu W, Yu Y, Ding J, Hua Z and Wang Y.2010a. Characterization of a novel stilbene synthase promoterinvolved in pathogen-and stress-inducible expression from Chinese wild Vitis pseudoreticulata. Planta,231(2):475-487
Xu Y, Zhu Z, Xiao Y and Wang Y.2009. Construction of a cDNA Library of Vitis pseudoreticulata Nativeto China inoculated with Uncinula necator and the analysis of potential defence-related expressedsequence Tags (ESTs). South African Society for Enology&Viticulture,30(1):65-71
Xu WR, Yu YH, Zhou Q, Ding JH, Dai LM, Xie XQ, Xu Y, Zhang CH and Wang YJ.2011. Expressionpattern, genomic structure, and promoter analysis of the gene encoding stilbene synthase from Chinesewild Vitis pseudoreticulata. Journal of Experimental Botany,62(8):2745-2761
Xue B, Ling K S, Reid C L.1999.Transformation of five grape rootstocks with plant virus genes and avirE2gene from Agrobacteriu tumefaciens. In Vitro Cellular and Developmental Biology Plant,35(3):226-231
Yamamoto T, Iketani H, Ieki H, Nishizawa Y, Notsuka K, Hibi T, Hayashi T, Matsuta N.2000.Transgenicgrapevine plants expressing a rice chitinase with enhanced resistance to fungal pathogens. Plant CellRep,19:639-646
Yu CKY, Springob K, Schmidt JR, Nicholson RL, Chu IK, Yip WK and Lo C.2005. A stilbene synthasegene (SbSTS1) is involved in host and nonhost defense responses in Sorghum. Plant Physiology,138(1):393-401
Zhang J, Wang Y, Wang X, Yang K and Yang J.2003. An Improved Method for Rapidly Extracting TotalRNA from Vitis. Journal of Fruit Science,20(3):178-181
Zhang J, Wang Y and Xu Y.2002. The Resistance of Chinese Wild Vitis to Uncinula necator and itsInheritance in F1Generation. Agricultural Sciences in China,1(2):195-199
© 2004-2018 中国地质图书馆版权所有 京ICP备05064691号 京公网安备11010802017129号 地址：北京市海淀区学院路29号 邮编：100083 电话：办公室：(+86 10)66554848；文献借阅、咨询服务、科技查新：66554700 |