重组人Ⅱ型肿瘤坏死因子受体—抗体融合蛋白对神经病理性疼痛大鼠痛敏的影响
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摘要
论文一重组人Ⅱ型肿瘤坏死因子受体-抗体融合蛋白对神经病理性疼痛大鼠行为学的影响
目的观察益赛普对神经病理性疼痛大鼠行为学的影响。方法成年雄性SD大鼠32只随机分为假手术组+NS组、CCI+NS组、CCI+0.4mg/kg益赛普组和CCI+0.8mg/kg益赛普组,于术后即刻开始至取材点每天给予皮下注射益赛普或生理盐水。于术前1d,术后1d、3d、7d测定机械性痛觉阈值(MWT)、热痛觉阈值(TWL)和运动功能评分。结果与假手术组相比,CCI组机械痛和热痛阈值在术后各时间点都明显下降(P<0.05)。益赛普组在术后各时间点机械痛和热痛阈值较CCI组都明显升高(P<0.05),且高剂量组较低剂量组有明显升高(P<0.05)。结论益赛普能够抑制CCI引起的神经病理性疼痛,且高剂量更有效。
论文二
第一部分重组人Ⅱ型肿瘤坏死因子受体-抗体融合蛋白对神经病理性疼痛大鼠脊髓中TNF-α免疫活性表达的影响
目的观察益赛普对神经病理性疼痛大鼠脊髓TNF-α免疫活性的影响。方法成年雄性SD大鼠32只随机分为假手术组+NS组、CCI+NS组、CCI+0.4mg/kg益赛普组和CCI+0.8mg/kg益赛普组,于术后即刻开始至取材点每天给予皮下注射益赛普或生理盐水。测定行为学后取腰段脊髓进行免疫组化测定。结果与假手术组相比,CCI组TNF-α免疫活性明显升高(P<0.05),与CCI组相比,益赛普组TNF-α免疫活性明显降低(P<0.05),与低剂量组相比,高剂量益赛普组TNF-α免疫活性明显降低(P<0.05)。结论益赛普能够抑制CCI大鼠脊髓TNF-α的免疫活性,且高剂量更有效。
第二部分RT-PCR检测神经病理性疼痛大鼠脊髓中TNF-αmRNA表达的变化及重组人Ⅱ型肿瘤坏死因子受体-抗体融合蛋白的作用
目的观察益赛普对神经病理性疼痛大鼠脊髓TNF-αmRNA表达的影响。方法成年雄性SD大鼠32只随机分为假手术组+NS组、CCI+NS组、CCI+0.4mg/kg益赛普组和CCI+0.8mg/kg益赛普组,于术后即刻开始至取材点每天给予皮下注射益赛普或生理盐水。测定行为学后取腰段脊髓进行RT-PCR测定。结果与假手术组相比,CCI组TNF-αmRNA表达明显升高(P<0.05),与CCI组相比,益赛普组TNF-αmRNA表达明显降低(P<0.05),与低剂量组相比,高剂量益赛普组TNF-αmRNA表达明显降低(P<0.05)。结论益赛普能够抑制CCI大鼠脊髓TNF-αmRNA的表达,且高剂量更有效。
The Effect Of Recombinant Human Tumor Necrosis Factor Receptor
TypeⅡ- antibody Fusion Protein On Behavior In Neuropathic Pain Rats.
1、Objective To investigate the effect of recombinant human tumor necrosis factor receptor typeⅡ- antibody fusion protein on behavior in neuropathic pain rats. Methods Thirty-two adult male SD rats were randomly divided into sham-operation group, chronic constriction injury (CCI) group, 0.4mg/kg and 0.8mg/kg etanercept groups, gave subcutaneous injection etanercept or NS every day from postoperative to derived point, determined mechanical withdrawal threshold(MWT)、thermal withdrawal latency(TWL)and motor function score in preoperative 1d, postoperative 1d、3d、7d.Results Compared with sham-operation group, the MWT and TWL of CCI group have significantly decrease in postoperative 1d、3d、7d(P<0.05).Compared with CCI group, the MWT and TWL of etanercept groups have significantly elevate at all postoperative time points(P<0.05), and high-dose group have significantly elevate than low-dose group (P<0.05).Conclusions Etanercept can inhibit neuropathic pain induced by CCI, and high-dose have more effective than low-dose.
2、
(1) Objective To investigate the effect of recombinant human tumor necrosis factor receptor typeⅡ- antibody fusion protein on TNF-αimmunocompetence of spinal cord in neuropathic pain rats. Methods Thirty-two adult male SD rats were randomly divided into sham-operation group, chronic constriction injury (CCI) group, 0.4mg/kg and 0.8mg/kg etanercept groups, gave subcutaneous injection etanercept or NS every day from postoperative to derived point, take lumber spinal cord to do Immunohistochemistry after determination of behavior. Results Compared with sham-operation group, the TNF-αimmunocompetence of CCI group have significantly elevate(P<0.05), Compared with CCI group, the TNF-αimmunocompetence of etanercept groups have significantly decrease(P<0.05), and high-dose group have significantly decrease than low-dose group ( P < 0.05 ) .Conclusions Etanercept can inhibit TNF-αimmunocompetence of spinal cord induced by CCI, and high-dose have more effective than low-dose.
(2) Objective To investigate the effect of recombinant human tumor necrosis factor receptor typeⅡ- antibody fusion protein on TNF-αmRNA of spinal cord in neuropathic pain rats. Methods Thirty-two adult male SD rats were randomly divided into sham-operation group, chronic constriction injury (CCI) group, 0.4mg/kg and 0.8mg/kg etanercept groups, gave subcutaneous injection etanercept or NS every day from postoperative to derived point, take lumber spinal cord to do RT-PCR after determination of behavior. Results Compared with sham-operation group, the TNF-αmRNA of CCI group have significantly elevat(eP<0.05), Compared with CCI group, the TNF-αmRNA of etanercept groups have significantly decrease(P<0.05), and high-dose group have significantly decrease than low-dose group ( P <0.05).Conclusions Etanercept can inhibit TNF-αmRNA of spinal cord induced by CCI, and high-dose have more effective than low-dose.
目的观察益赛普对神经病理性疼痛大鼠行为学的影响。方法成年雄性SD大鼠32只随机分为假手术组+NS组、CCI+NS组、CCI+0.4mg/kg益赛普组和CCI+0.8mg/kg益赛普组,于术后即刻开始至取材点每天给予皮下注射益赛普或生理盐水。于术前1d,术后1d、3d、7d测定机械性痛觉阈值(MWT)、热痛觉阈值(TWL)和运动功能评分。结果与假手术组相比,CCI组机械痛和热痛阈值在术后各时间点都明显下降(P<0.05)。益赛普组在术后各时间点机械痛和热痛阈值较CCI组都明显升高(P<0.05),且高剂量组较低剂量组有明显升高(P<0.05)。结论益赛普能够抑制CCI引起的神经病理性疼痛,且高剂量更有效。
论文二
第一部分重组人Ⅱ型肿瘤坏死因子受体-抗体融合蛋白对神经病理性疼痛大鼠脊髓中TNF-α免疫活性表达的影响
目的观察益赛普对神经病理性疼痛大鼠脊髓TNF-α免疫活性的影响。方法成年雄性SD大鼠32只随机分为假手术组+NS组、CCI+NS组、CCI+0.4mg/kg益赛普组和CCI+0.8mg/kg益赛普组,于术后即刻开始至取材点每天给予皮下注射益赛普或生理盐水。测定行为学后取腰段脊髓进行免疫组化测定。结果与假手术组相比,CCI组TNF-α免疫活性明显升高(P<0.05),与CCI组相比,益赛普组TNF-α免疫活性明显降低(P<0.05),与低剂量组相比,高剂量益赛普组TNF-α免疫活性明显降低(P<0.05)。结论益赛普能够抑制CCI大鼠脊髓TNF-α的免疫活性,且高剂量更有效。
第二部分RT-PCR检测神经病理性疼痛大鼠脊髓中TNF-αmRNA表达的变化及重组人Ⅱ型肿瘤坏死因子受体-抗体融合蛋白的作用
目的观察益赛普对神经病理性疼痛大鼠脊髓TNF-αmRNA表达的影响。方法成年雄性SD大鼠32只随机分为假手术组+NS组、CCI+NS组、CCI+0.4mg/kg益赛普组和CCI+0.8mg/kg益赛普组,于术后即刻开始至取材点每天给予皮下注射益赛普或生理盐水。测定行为学后取腰段脊髓进行RT-PCR测定。结果与假手术组相比,CCI组TNF-αmRNA表达明显升高(P<0.05),与CCI组相比,益赛普组TNF-αmRNA表达明显降低(P<0.05),与低剂量组相比,高剂量益赛普组TNF-αmRNA表达明显降低(P<0.05)。结论益赛普能够抑制CCI大鼠脊髓TNF-αmRNA的表达,且高剂量更有效。
The Effect Of Recombinant Human Tumor Necrosis Factor Receptor
TypeⅡ- antibody Fusion Protein On Behavior In Neuropathic Pain Rats.
1、Objective To investigate the effect of recombinant human tumor necrosis factor receptor typeⅡ- antibody fusion protein on behavior in neuropathic pain rats. Methods Thirty-two adult male SD rats were randomly divided into sham-operation group, chronic constriction injury (CCI) group, 0.4mg/kg and 0.8mg/kg etanercept groups, gave subcutaneous injection etanercept or NS every day from postoperative to derived point, determined mechanical withdrawal threshold(MWT)、thermal withdrawal latency(TWL)and motor function score in preoperative 1d, postoperative 1d、3d、7d.Results Compared with sham-operation group, the MWT and TWL of CCI group have significantly decrease in postoperative 1d、3d、7d(P<0.05).Compared with CCI group, the MWT and TWL of etanercept groups have significantly elevate at all postoperative time points(P<0.05), and high-dose group have significantly elevate than low-dose group (P<0.05).Conclusions Etanercept can inhibit neuropathic pain induced by CCI, and high-dose have more effective than low-dose.
2、
(1) Objective To investigate the effect of recombinant human tumor necrosis factor receptor typeⅡ- antibody fusion protein on TNF-αimmunocompetence of spinal cord in neuropathic pain rats. Methods Thirty-two adult male SD rats were randomly divided into sham-operation group, chronic constriction injury (CCI) group, 0.4mg/kg and 0.8mg/kg etanercept groups, gave subcutaneous injection etanercept or NS every day from postoperative to derived point, take lumber spinal cord to do Immunohistochemistry after determination of behavior. Results Compared with sham-operation group, the TNF-αimmunocompetence of CCI group have significantly elevate(P<0.05), Compared with CCI group, the TNF-αimmunocompetence of etanercept groups have significantly decrease(P<0.05), and high-dose group have significantly decrease than low-dose group ( P < 0.05 ) .Conclusions Etanercept can inhibit TNF-αimmunocompetence of spinal cord induced by CCI, and high-dose have more effective than low-dose.
(2) Objective To investigate the effect of recombinant human tumor necrosis factor receptor typeⅡ- antibody fusion protein on TNF-αmRNA of spinal cord in neuropathic pain rats. Methods Thirty-two adult male SD rats were randomly divided into sham-operation group, chronic constriction injury (CCI) group, 0.4mg/kg and 0.8mg/kg etanercept groups, gave subcutaneous injection etanercept or NS every day from postoperative to derived point, take lumber spinal cord to do RT-PCR after determination of behavior. Results Compared with sham-operation group, the TNF-αmRNA of CCI group have significantly elevat(eP<0.05), Compared with CCI group, the TNF-αmRNA of etanercept groups have significantly decrease(P<0.05), and high-dose group have significantly decrease than low-dose group ( P <0.05).Conclusions Etanercept can inhibit TNF-αmRNA of spinal cord induced by CCI, and high-dose have more effective than low-dose.
引文
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2. Chaplan S, Bach F, Pogrel J, et al. Quantitative assessment of tactile allodynia in the rat paw. J Neurosci Methods, 1994,53:55–63.
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