栝蒌子功能性脂类的研究
|
摘要
本研究以栝蒌子为原料,利用超临界CO_2萃取技术、低温富集技术、微胶囊技术、高通量筛选等现代高新食品科学技术,从栝蒌子中提取栝蒌子油、富集纯化栝蒌子功能性脂类,并对其功效成分进行了微胶囊化研究。探讨了微胶囊化生产的工艺技术,并系统地研究了栝蒌子功能性脂类理化性质、贮藏稳定性和体内、体外的生理活性。主要研究内容如下:
1、超临界CO_2萃取栝蒌子油技术的研究以栝蒌子为原料,研究了压力、温度、时间等因素对萃取率的影响,得出最佳的萃取条件:萃取压力为35Mpa,分离压力为6～10Mpa,萃取温度为45℃,分离温度为30℃,CO_2流量为80L/h,萃取2.5h,对萃取率影响由大到小依次为萃取温度、时间、压力。萃取的主要物质为亚油酸(37.137%),亚麻油酸(9.274%),a-亚麻酸(5.43%)等功能性成分,多不饱和脂肪酸总含量约达到约52%(为1#样品);而按传统的方法提取的多不饱和脂肪酸总含量约为6%左右。
2、用低温浓缩技术提取富集多不饱和脂肪酸
以超临界二氧化碳萃取的栝蒌子功能性脂类1#样品为对象,利用低温浓缩法富集高浓度多不饱和脂肪酸;并利用气相色谱法测定其中的多不饱和脂肪酸,主要针对温度、时间、溶剂配比等条件进行比较,结果表明:-70℃、原油:溶剂为1:10、冷冻2h,多不饱和脂肪酸含量提高到83.05%。结论:该工艺简便、无污染,利于工业化生产,此为栝蒌子功能性脂类2#样品。
3、栝蒌子功能性脂类的稳定性研究
研究了光线、温度、紫外线以及储存时间等因素对栝蒌子功能性脂类2#样品所产生的影响,结果表明,这些因素对栝萎子功能性脂类的氧化均有显著影响,能大大加快栝蒌子功能性脂类的氧化速度。通过添加抗氧化剂BHA、BHT、PG、TBHQ以及增效剂柠檬酸来提高其抗氧化性能,结果表明:0.01%TBHQ+0.01%BHT+0.01%柠檬酸作为栝蒌子功能性脂类的复合抗氧化剂,可使栝蒌子功能性脂类在60℃条件下的贮藏时间由8d延长到26d,在20℃条件下的预期贮藏时间从4个月延长至14个月,储存时间显著延长。
4.栝蒌子功能性脂类的微胶囊技术研究
采用喷雾干燥法对2#样品进行微胶囊化,确定了所选用的复合壁材,并对影响微胶囊化的各种因素,如芯材与壁材配比、复合壁材配比、总固形物含量以及喷雾干燥进出口温度和均质压力进行了研究。研究结果表明,在微胶囊化生产过程研究中,选择麦芽糊精和阿拉伯胶作为复合壁材,对微胶囊包埋率影响大小为芯材与壁材配比)复合壁材配比)总固形物含量。正交试验所得微胶囊最佳配比为:阿拉伯胶与麦芽糊精的配比1:1,芯材与壁材比率2:3,总固形物含量25%(W/V)。喷雾干燥的最佳工艺条件为:进风温度180℃,出风温度80℃,均质压为35MPa。微胶囊对油脂具有较好的包埋效果,对芯材具有很好的保护作用,经微胶囊化处理后的栝蒌子油氧化稳定性显著增强。
5、栝蒌子功能性脂类生理活性的高通量筛选
利用栝萎子功能性脂类1#和2#样品为实验材料,对栝蒌子功能性脂类进行了PPAR模型的高通量筛选,实验证明:栝蒌子功能性脂类2#对PPARα、PPARγ受体的激活作用很弱,对PPAR6作用显著,而1#样品对这三种模型则没有作用。
6.栝蒌子功能性脂类体内生理活性研究
利用栝蒌子功能性脂类2#样品作为研究对象,通过降脂功能方面的体外实验,选用高脂饲养诱导的高脂血症大鼠为模型,治疗性灌胃给予栝蒌子功能性脂类2#样品,剂量为每日0.5、1和3mg/kg共4周,结果发现:2#样品能明显降低大鼠总胆固醇和低密度脂蛋白水平,但对各组的TG和HDL水平无明显改变。
Studies on using Trichosanthes Kirilowii Maxim seed as raw materials and applying modern high-tech food engineering technology,mainly supercritical CO_2 extraction technology,cryoconcentration method.the soft capsule technic,The High Throughput Screening Method etc.Extracted and separated the oil from Trichosanthes Kirilowii Maxim seed and enriched and purified poly—unsaturated fatty acids. discussed the arts and crafts of the soft capsule of the oil of Trichosanthes Kirilowii Maxim seed,investigated its physical and chemical character ,the stability and the activity.The main results of this study were as follows:
1.Study on supercritical CO_2 extraction technology of function fat of Trichosanthes Kirilowii Maxim seed
Trichosanthes Kirilowii Maxim seed is the material of study, and pressure,temperature and time is the factor of effect. The best technical condition of extraction are: the pressure of extraction is 35M P a , the pressure of separation is 6～10Mpa,the tempreture of extraction is 45℃, the tempreture of separation is 30℃,the flux of CO_2 is 80l/ h ,the time is 2.5 h .The influence of rate of extraction is temperature,time,pressure in turn from bigness to smallness.The main substance are linoleic acid (37.137%) ,flaxoleic acid (9.2745%),a-linolenic acid (5.43%) and so on. and The content of poly—unsaturated fatty acids with the extraction of supercritical CO_2 fluidextraction is about 52%(by way of 1#).But the content of poly—unsaturated fatty acids with the method of extraction of tradition is about 22%.
2. Enrich poly—unsaturated fatty acids by cryoconcentration technology
Purpose to extraction and to enrich poly—unsaturated fatty acids by cryoconcentration technology. Methods natural Trichosanthes Kirilowii Maxim seed primrose oil extracted by supercritical fluid was used as raw material. High concentration of poly—unsaturated fatty acids was enriched by cryoconcentration method .poly—unsaturated fatty acids was determined by GC,and different temperatures,time and solvent proportions were compared.Results:-70℃,raw oil :solvent=1:8,freezing 2h,the content of poly—unsaturated fatty acids was raised to 83.05%.The technology is simple and convenient without any solvent pollution.It is beneficial to apply in industrial production, by way of 2#.
3. Study on stability of function fat of Trichosanthes Kirilowii Maxim seed
The ray ,temperature ,ultraviolet radiation and time on influence of function component of Trichosanthes Kirilowii Maxim seed(2#) . The influence of these factors are market.The speed is rapid. there is abundant of poly—unsaturated fatty acids in the oil of Trichosanthes Kirilowii Maxim seed, it is easy oxidated very much .the main content of paper are discussing the effect to improve its antioxidation by adding BHA、BHT、PG、TBHQ as well as citric acid.The result are:when we added 0.01%TBHQ + 0.01%BHT+ 0.01% Citric acid, the shelf life of the oil of Trichosanthes Kirilowii Maxim seed is extended from 8 days to 26 days at 60℃, the shelf life of the oil of Trichosanthes Kirilowii Maxim seed is extended from 4 months to 14 months at 20℃,the effect is very distinct.
4. Study of microcapsule of the function fat of Trichosanthes Kirilowii Maximseed
Spray-drying is needed for microcapsule of 2# capsules research,identified a selected compound wall material, and the impact of Trapa natans seed oil microcapsules of various factors such as the ratio of core material and wall material ,the ratio of compound wall material and the total solids content, as well as import and export of spray-dried temperature and homogenizing pressure were studied. The results show that in the study of the Trapa natans seed oil microcapsule production process, maltodextrin and arabic gum is been selected for composite wall material , the impact of embedding rate of various factors is the ratio of core material and wall material >the ratio of compound wall material > the total solids content .Orthogonal design from the best ratio of microencapsulation as follows: the ratio of arabic gum and maltodextrin is 1 : 1, the ratio of core material and wall material is 2 : 3, the total solids content is 25% (W / V). The optimum spray drying conditions as follows: the temperature of entering wind is 180℃,the temperature of out wind is 80℃,the homogenizing pressure is 35MPa. microencapsulation with a better embedding,microencapsulation of core material has a good protective effect, Microencapsulation enhance the oxidation stability of 2#.
5. Throughput Screening on the pharmacological activity in function fat of Trichosanthes Kirilowii Maxim seed
Studying the pharmacological activity in function component of Trichosanthes Kirilowii Maxim seed(1#and2#) extraction by High Throughput Screening method . High Throughput Screening method is the main tehnology to screen drugs currutly, and is used widely by many foreign drugs research institutes because of the characteristics of micro-quantity speediness and high efficiency. This paper used PPAR model of High Throughput Screening being the first time in Trichosanthes Kirilowii Maxim seed study to screen active compounds of Trichosanthes Kirilowii Maxim seed. The result showed that a function component of Trichosanthes Kirilowii Maxim seed(2#) acted on PPARαand PPARγfaintly, PPARδstronger,but 1# sample have no activity to the three model.
6.Study on the pharmacological activity of body of function fat of Trichosanthes Kirilowii Maxim seed
Lowering blood-lipid investigation with function fat of Trichosanthes Kirilowii Maxim seed 2#,Using high blood-lipid big-rat model which were brood by high-fat feed, then were filled stomach by function component of Trichosanthes Kirilowii Maxim seed( 2#)(0.2、0.4 and 0.8 mg/g per day,four weeks).The dose can depress LDLand TC,but TG and HDL hardly influence.
1、超临界CO_2萃取栝蒌子油技术的研究以栝蒌子为原料,研究了压力、温度、时间等因素对萃取率的影响,得出最佳的萃取条件:萃取压力为35Mpa,分离压力为6～10Mpa,萃取温度为45℃,分离温度为30℃,CO_2流量为80L/h,萃取2.5h,对萃取率影响由大到小依次为萃取温度、时间、压力。萃取的主要物质为亚油酸(37.137%),亚麻油酸(9.274%),a-亚麻酸(5.43%)等功能性成分,多不饱和脂肪酸总含量约达到约52%(为1#样品);而按传统的方法提取的多不饱和脂肪酸总含量约为6%左右。
2、用低温浓缩技术提取富集多不饱和脂肪酸
以超临界二氧化碳萃取的栝蒌子功能性脂类1#样品为对象,利用低温浓缩法富集高浓度多不饱和脂肪酸;并利用气相色谱法测定其中的多不饱和脂肪酸,主要针对温度、时间、溶剂配比等条件进行比较,结果表明:-70℃、原油:溶剂为1:10、冷冻2h,多不饱和脂肪酸含量提高到83.05%。结论:该工艺简便、无污染,利于工业化生产,此为栝蒌子功能性脂类2#样品。
3、栝蒌子功能性脂类的稳定性研究
研究了光线、温度、紫外线以及储存时间等因素对栝蒌子功能性脂类2#样品所产生的影响,结果表明,这些因素对栝萎子功能性脂类的氧化均有显著影响,能大大加快栝蒌子功能性脂类的氧化速度。通过添加抗氧化剂BHA、BHT、PG、TBHQ以及增效剂柠檬酸来提高其抗氧化性能,结果表明:0.01%TBHQ+0.01%BHT+0.01%柠檬酸作为栝蒌子功能性脂类的复合抗氧化剂,可使栝蒌子功能性脂类在60℃条件下的贮藏时间由8d延长到26d,在20℃条件下的预期贮藏时间从4个月延长至14个月,储存时间显著延长。
4.栝蒌子功能性脂类的微胶囊技术研究
采用喷雾干燥法对2#样品进行微胶囊化,确定了所选用的复合壁材,并对影响微胶囊化的各种因素,如芯材与壁材配比、复合壁材配比、总固形物含量以及喷雾干燥进出口温度和均质压力进行了研究。研究结果表明,在微胶囊化生产过程研究中,选择麦芽糊精和阿拉伯胶作为复合壁材,对微胶囊包埋率影响大小为芯材与壁材配比)复合壁材配比)总固形物含量。正交试验所得微胶囊最佳配比为:阿拉伯胶与麦芽糊精的配比1:1,芯材与壁材比率2:3,总固形物含量25%(W/V)。喷雾干燥的最佳工艺条件为:进风温度180℃,出风温度80℃,均质压为35MPa。微胶囊对油脂具有较好的包埋效果,对芯材具有很好的保护作用,经微胶囊化处理后的栝蒌子油氧化稳定性显著增强。
5、栝蒌子功能性脂类生理活性的高通量筛选
利用栝萎子功能性脂类1#和2#样品为实验材料,对栝蒌子功能性脂类进行了PPAR模型的高通量筛选,实验证明:栝蒌子功能性脂类2#对PPARα、PPARγ受体的激活作用很弱,对PPAR6作用显著,而1#样品对这三种模型则没有作用。
6.栝蒌子功能性脂类体内生理活性研究
利用栝蒌子功能性脂类2#样品作为研究对象,通过降脂功能方面的体外实验,选用高脂饲养诱导的高脂血症大鼠为模型,治疗性灌胃给予栝蒌子功能性脂类2#样品,剂量为每日0.5、1和3mg/kg共4周,结果发现:2#样品能明显降低大鼠总胆固醇和低密度脂蛋白水平,但对各组的TG和HDL水平无明显改变。
Studies on using Trichosanthes Kirilowii Maxim seed as raw materials and applying modern high-tech food engineering technology,mainly supercritical CO_2 extraction technology,cryoconcentration method.the soft capsule technic,The High Throughput Screening Method etc.Extracted and separated the oil from Trichosanthes Kirilowii Maxim seed and enriched and purified poly—unsaturated fatty acids. discussed the arts and crafts of the soft capsule of the oil of Trichosanthes Kirilowii Maxim seed,investigated its physical and chemical character ,the stability and the activity.The main results of this study were as follows:
1.Study on supercritical CO_2 extraction technology of function fat of Trichosanthes Kirilowii Maxim seed
Trichosanthes Kirilowii Maxim seed is the material of study, and pressure,temperature and time is the factor of effect. The best technical condition of extraction are: the pressure of extraction is 35M P a , the pressure of separation is 6～10Mpa,the tempreture of extraction is 45℃, the tempreture of separation is 30℃,the flux of CO_2 is 80l/ h ,the time is 2.5 h .The influence of rate of extraction is temperature,time,pressure in turn from bigness to smallness.The main substance are linoleic acid (37.137%) ,flaxoleic acid (9.2745%),a-linolenic acid (5.43%) and so on. and The content of poly—unsaturated fatty acids with the extraction of supercritical CO_2 fluidextraction is about 52%(by way of 1#).But the content of poly—unsaturated fatty acids with the method of extraction of tradition is about 22%.
2. Enrich poly—unsaturated fatty acids by cryoconcentration technology
Purpose to extraction and to enrich poly—unsaturated fatty acids by cryoconcentration technology. Methods natural Trichosanthes Kirilowii Maxim seed primrose oil extracted by supercritical fluid was used as raw material. High concentration of poly—unsaturated fatty acids was enriched by cryoconcentration method .poly—unsaturated fatty acids was determined by GC,and different temperatures,time and solvent proportions were compared.Results:-70℃,raw oil :solvent=1:8,freezing 2h,the content of poly—unsaturated fatty acids was raised to 83.05%.The technology is simple and convenient without any solvent pollution.It is beneficial to apply in industrial production, by way of 2#.
3. Study on stability of function fat of Trichosanthes Kirilowii Maxim seed
The ray ,temperature ,ultraviolet radiation and time on influence of function component of Trichosanthes Kirilowii Maxim seed(2#) . The influence of these factors are market.The speed is rapid. there is abundant of poly—unsaturated fatty acids in the oil of Trichosanthes Kirilowii Maxim seed, it is easy oxidated very much .the main content of paper are discussing the effect to improve its antioxidation by adding BHA、BHT、PG、TBHQ as well as citric acid.The result are:when we added 0.01%TBHQ + 0.01%BHT+ 0.01% Citric acid, the shelf life of the oil of Trichosanthes Kirilowii Maxim seed is extended from 8 days to 26 days at 60℃, the shelf life of the oil of Trichosanthes Kirilowii Maxim seed is extended from 4 months to 14 months at 20℃,the effect is very distinct.
4. Study of microcapsule of the function fat of Trichosanthes Kirilowii Maximseed
Spray-drying is needed for microcapsule of 2# capsules research,identified a selected compound wall material, and the impact of Trapa natans seed oil microcapsules of various factors such as the ratio of core material and wall material ,the ratio of compound wall material and the total solids content, as well as import and export of spray-dried temperature and homogenizing pressure were studied. The results show that in the study of the Trapa natans seed oil microcapsule production process, maltodextrin and arabic gum is been selected for composite wall material , the impact of embedding rate of various factors is the ratio of core material and wall material >the ratio of compound wall material > the total solids content .Orthogonal design from the best ratio of microencapsulation as follows: the ratio of arabic gum and maltodextrin is 1 : 1, the ratio of core material and wall material is 2 : 3, the total solids content is 25% (W / V). The optimum spray drying conditions as follows: the temperature of entering wind is 180℃,the temperature of out wind is 80℃,the homogenizing pressure is 35MPa. microencapsulation with a better embedding,microencapsulation of core material has a good protective effect, Microencapsulation enhance the oxidation stability of 2#.
5. Throughput Screening on the pharmacological activity in function fat of Trichosanthes Kirilowii Maxim seed
Studying the pharmacological activity in function component of Trichosanthes Kirilowii Maxim seed(1#and2#) extraction by High Throughput Screening method . High Throughput Screening method is the main tehnology to screen drugs currutly, and is used widely by many foreign drugs research institutes because of the characteristics of micro-quantity speediness and high efficiency. This paper used PPAR model of High Throughput Screening being the first time in Trichosanthes Kirilowii Maxim seed study to screen active compounds of Trichosanthes Kirilowii Maxim seed. The result showed that a function component of Trichosanthes Kirilowii Maxim seed(2#) acted on PPARαand PPARγfaintly, PPARδstronger,but 1# sample have no activity to the three model.
6.Study on the pharmacological activity of body of function fat of Trichosanthes Kirilowii Maxim seed
Lowering blood-lipid investigation with function fat of Trichosanthes Kirilowii Maxim seed 2#,Using high blood-lipid big-rat model which were brood by high-fat feed, then were filled stomach by function component of Trichosanthes Kirilowii Maxim seed( 2#)(0.2、0.4 and 0.8 mg/g per day,four weeks).The dose can depress LDLand TC,but TG and HDL hardly influence.
引文
[1].Chen S-K(陈书坤),Wu Z-Y(吴征镒),Chen Z-L(陈宗莲)1986 Trichosanthes.Flora Reipublicae Popularis Sinicae(中国植物志) Beijing Science Press 73:223-255
[2].Huang LQ(黄璐琦) 1995.Study on the systematics of Trichosanthes L(Cucurbitaceae).The Doctor Degree of Medical Science Department of Beijing University
[3].Kurz,S.Contribution towards alhowlcdge of Burmese flora.Journ.AS.Soc Beng 1877,46(2)97-99
[4].Yue C-X(乐崇熙),Chen J-R(诚静容).Apreliminary study on the Chinese medicinal species of genus Trichosanthes L Acta Phytotaxonomica Sinica(植物分类学报) 1974,12:415-446
[5].Yue C-X(乐崇熙),Chen J-R(诚静容).The Chinese medicinal species of the genus Trichosanthes.L Actr Phytotaxonomica Sinica(植物分类学报) 1980,18:333-352
[6].黄璐琦,桔蒌属(Trichosanthes)药用植物的细胞学及根的化学鉴别研究,中国中医研究院硕士学位论文,1992
[7].Yue C-X(乐崇熙),Zhang Y-L(张玉龙)1986 Studies on the pollen motholo of chinese Trichosathes.Bulletin of Botanical Research(植物研究) 6:21-35
[8].Huang L-Q(黄璐琦),Yue C-X(乐崇熙),Li M-X(李懋学)Chromosome numbers of 8 species in Trichosanthes L Acta Botanica Yunnanica(云南植物研究) 199416:95-96
[9].Bhaduri P N Cytogenetical investigations in some chromosomes as a physical basis of speciation.Joural Genetics 1947,48:237-256
[10].Rangaswami K,Tatuno,Yoshida Scx chromosomes of Trichosanthes palmate Roxb Proceedings of Indian Science,1949,3:137
[11].Darlington CD,1955,Chromosome altas of flowering plants.London G Aeeen Press.
[12].Thakur GK Cytology of a tetraploid Trichosanthes bracteata(Lam).Voigt from Bihar Journal Cytologic Genetics 1976,11:47-50
[13].Singh A K Cytological studies in Trichosanthes L Journal Cytogenetics,1979,14:50-57
[14].Pandcy V Thompson W.B Chromosome in Trichosanthes lepiniana and Cogn.Current Science 1986,55:159
[15].E.B Byjib,1960 An introduction to historical plant geography Beijing:Science Press
[16]黄璐琦,杨滨,乐崇熙.括蒌属药用植物资源调查.中国中药杂志,1995,20(4):195-197
[17]陈书坤.中国括蒌属的新分类群.植物研究,1985,5(2):113-121
[18]蒌之岑,秦波.常用中药材品种整理和质量研究.北京:北京医科大学、中国协 和医科大学联合出版社,1996,585
[19]陈胜发,黄时伟,王昌利.括蒌属植物化学成分的研究进展.中成药,2006,28(8):1187-1192
[20]巢志茂,刘静明.湖北括萎果皮挥发油化学成分的研究.中国药学杂志,1996,31(3):140-141
[21]霍启录,陈振德,许重远.双边括蒌种子油含量及其脂肪酸的测定.华西药学杂志,2002,17(5):351-352
[22]巢志茂,刘静明.双边括蒌中括蒌碱的结构研究.药学学报,1995,30(7):517-520
[23]巢志茂,刘静明.双边括萎皮挥发油的化学成分研究.中国中药杂志,21(6):357-384
[24]时岩鹏,姚庆强,刘拥军.括蒌化学成分的研究及其菠菜昌醇的含量测定.中草药,2002,33(1):14-16
[25]巢志茂,何波.括蒌果实的化学成分研究.中国中药杂志,1999,24(10):612-613
[26]巢志茂,何波,张颖.括蒌种子中不皂化类脂的化学成分研究.中国药学杂志,2000,35(11):131-135
[27]黄民权.括蒌属植物种子中特殊脂肪酸成分的研究.植物研究,1986,6(1):79-83
[28]胡合妓,王鸿,潘远江.GC-MS法测定余杭括萎根块的挥发油成分.林产化学与工业,2005,25(1):109-111
[29]何祥久,印峰,姚新生,等.括蒌属植物化学成分.国外医药·植物药分册,2002,17(1):11-13
[30]ItohT,YashidaK,TarnuraT,etal.3thyl-sterolsin the roots of Triehosanthes joniea,chemistiy,1982,21(3):727
[31]AkihisaT,TamuraT,MatsumotoT.Karounidiol and its 3benzoate:novel Pentaey clietri terpenes from Trichosan the skirilowi ray molecular settireof karouni diol diacetate.J Chem Soc Perkin Transl Org Chem,1988,(3):439
[32]AkihisaT,Kokke WCMC Kimuraet first uaturall yal.Isokarounidio the occurring triterpene with6,8-conjugateddienesystem.iodine mediated dedrogenation and isomeri Zationof its diacetate.JO Chem,1993,58(7):1959
[33]李丰.括蒌籽中一种新的具有蛋白质生物合成抑制活性的多肤-Trichokirin-S1的分离、纯化和性质.生物化学与生物物理学报,2003,35(9):841-845
[34]陈毓荃,吕志强,吴伸.括蒌籽核糖体失活蛋白(TCK)与天花粉蛋白(TCS)'胜质比较研究.西北植物学报,1998,18(3):423
[35]邱明华,陈书坤,陈剑超,等.葫芦科化学分类学.应用与环境生物学报,2005,11(6):673-68
[36]HilditehTP,etal.Fatty acids and glycerides of solid seed fats VIII:the seed fat of Hodgsonia eapnio carpa.JSoeChemhid,1939,58:27-28
[37]樊娟,冯宝树,邱明华,等.臭瓜根的化学成分.云南植物研究,1988,10(4):475-479.
[38]Dubo is MA,BauerR,Cagiotti MR and Wa gner H.Foeti dissimoside A,anew3,2-bidesmosi dictriter penoid saponin,and eueur bit aeins from Cucur bita foeti dissima.Phyto-chemistry,1988,27(3):881-885
[39]贝伟剑.瓜蒌建白汤药理研究概况.广西中医药,1998,18(3):423
[40]黄美兰,贝伟剑.大子括蒌和括蒌的药理作用比较.广东药学,2000,10(1):47
[41]AkihisaT,hsukawaK,KimuraY,etal.karounidiol.括蒌中的一种新的三萜.国外医学中医中药分册,1994,16(1):41
[42]邵春丽,王世久,王进.瓜蒌皮抗缺氧作用的研究.沈阳药科大学学报,1998,1998,15(1):38-31
[43]凌宏,鲁翔,董传仁.瓜蒌、心得安对血小板体外聚集和TXAZ合成的影响.湖北医学院学报,1988,9(2):138
[44]汪酞,金善炜.2000,天花粉蛋白.北京:科学出版社
[45]玄焕开,刘普,王菊.葫芦素的研究概况.齐鲁药事,2005,24(12):737-739
[46]秦林,高伟良.瓜蒌对子宫颈癌细胞和巨噬细胞的影响.山东中医学院学报,1995,19(6):414-417
[47]卢兆华.抗癌植物-括蒌.福建热作科技,1995,20(1):28-29
[48]贝伟剑,宋黎纳,赵一大子括蒌的药理作用.时珍国医国药研究,2002,6(2):20-22
[49]曹焕生,徐明芳,李定坚.大苞括蒌的药用价值分析.药物生物技术,2002,9(5):292-296
[50]彭桂福,李彩琴.食用括蒌的生育特性及栽培技术.中国西瓜甜瓜,2003,(4):44-40
[51]任静,何家庆.括蒌子资源的利用及其价值.中国野生植物资源,2000,20(6):21-22
[52]洪金祥,赵长江.多用途多效能的垂直绿化材料-括蒌.林业科技通迅,1994,(11):42
[53]杨廷祯,高敬东,杨明霞.括蒌的经济价值及栽培技术要点。甘肃农业科技,2003,(10):48-50.
[54]郎进宝,张贤朝,王珍儿.括蒌的特性及其丌发利用.上海农业科技,2004,(2):91
[55].Wang YQ LaiBS Yan XL,et al Analys is the composition of fatty acid from perilla frutiscens seeds and linseed with GC/MS[J]Chin I Biochem Pham(中国生化药物志 ),1999,20(2):62-64
[56].Yin L J,Wang Y Zu Y G Purification of linolenic acid from fruit oil of Camptotheca acuminata[J].Chin Trad it Herb Drugs(中草药),2002,33(1):22-23
[57].Ezaki O Takahashi M Shigem atsu T etal Long+trem effects of dietary alpha linolenic acid from perilla oil on serum fatty acids corn position and on the fisk factors of coronary heart disease in Japanese elderly subjects[J]Nutr Scivitaminol.1999,45(6):759-772
[58].Renaus S Lanzmann-petithory D Dietary fats and coronary heart disease pathogenesis[J].Curr A theroscler Rep,2002,4(6):419-424
[59].Ding G L,Hu DY,Wang B Suppression of transient out ward current by linolenic acid in atria myocytes from patienst with heart failure[J].Bullmed Res(医药研究通讯) 2000,29(6):8-9
[60].Lin H,Shen JQ,Xin D etal Effects of flaxseed on blood lipids on rats[J]I Capita LUniu Med Sci(首都医科大学学报),2001,22(2):104-106
[61].Chen J,Stavro P M,Thompson L U,Dietary flaxseed inhibits human breast cancer grow thand metastasis and down regulated expression of insulin like growth factor and epidermal grow th factor receptor[J].Nutr Cancer,2002,43(2):187-192
[62].Xie L T,Huang JQ Cytotoxic action and effect of unsaturated fatty acids and their selenidses on DNA synthes is in BEL-7402 human hepatocarcinom a cell[J]I Chin Tumour Clin(中国肿瘤临床),1998,25(7):501-504
[63].Lee J Y,Kim Y S,Shin D H.Anti microbial synerg is ticeffect of linolenic acid and monoglyceride against Bacillus cereus and Staphylococcus aureus[J].Agric Food Chem,2002,50(7):2193-2199
[64]. Kakus, Yunokis, Ohkura K etal Interactions of dietary fats and proteins on fatty acid composition of immune cells and LTB4 production by peritoneal exudates cells of rats [J] Biosci B iotechnol Biochem, 2001,65(2):315-321
[65]. Suresh Y Das U N Long-chain polyunsaturated fatty acids and chem. Ically induced diabetes mellitus. Effect of o mega-3 fatty acids [J] Nutrition, 2003,19(3):213-228
[66]. Ikem oto A Fukuma A, Fujii Y, etal Lysosomal enzyme activities are decreased in the retina and their circadian rhythms are different from those in the pineal gland of rats fed and alpha linolenic acid restricted diet [J] Nutrition 2000,130(12):3059-3062
[67]. Xu ZH Shao Y F Effect of linolenic acid on rat's behavior, retina, and liver, brain fatty acid composition [J]. Chin Public Health(中国公共卫生),2002,18 (3): 301-304
[68]. Umezawa M Kogishik, Tojo H etal High-linoleate and high-alpha-linolenate diets affect learning ability and natural behavior in SAMRL mice [J] Nutrition, 1999, 129(2):431-437
[69]. Zhang H M, Liu F Z, Dai L M ,Purity method of linolenic acid on the theory of urea adduction fractionation (I)-study on the noticeable factors affecting purification behavior of linolenic acid [J] Chin Lipid,2001,26(2):41-44
[70]. Zhu D. Wang S C Guo Y etal Purification of linolenic acid from Perilla frutiscens oil [J] Chin Tradit Herb Drugs 1997,28(6):242-243
[71]. Wang W, LiM J, Liu J H, etal Research on the quality standardization criterion on perilla frutiscens. Preparation and identification ethanol linolenic ester [J]. Chin Tradit Herb Drugs, 1999,30(4):267-268
[72]. Szentm ihalyik, Vinkler P, Lakatos B etal Rose hip(Rosa canina L)Oil obtained from waste hip seeds by different extraction methods [J]. Bioresour Technol, 2002,82(2):195-201
[73]. Shen X J, Lai B S, Chen Z T, etal, Effec of time of super critical fluid extraction on quality of fatty acids in plant medicines oil [J] Chin I pharm 2002,33(11):533-535
[74]. Chen Y Yang J C Studies on supercritical carbon dioxide extraction of linseed iol[J]. Nat Prod ResDEV,2002,13(3):14-19
[75]. Fu H Gan A Y Preparation of polyunsaturated fatty acid from fish oil with molecular distillation[J]I Wuxi univ Light Ind 200,21(6):617-621
[75]汪言满,陈达美,黎碧娜.超临界CO_2流体萃取技术在食品工业中的新应用[J].广州化工,20000,28(4):44-45
[76]彭阳生编著.植物油脂加工实用技术.北京:金盾出版社,2003
[78]韩国麒编著.油脂化学.郑州:河南科学技术出版社,1995
[1]沈晓京,赖炳森,陈镇童,等.超临界萃取时间对植物药油脂产率和脂肪酸质量的影响[J].中国医药工业杂志,2002,33(11):533-535
[2]Alkiom,Gonzalezc,Janttim,etal.Purl flcation of poly unsaturated fatty acid sesters from Tuna with superoritical fluid[J].Chromatography.JAOCS,2000,77(3):315-321
[3]李章万,周同惠.香薷挥发油成分的研究[J].药学学报,1983.18(5):363-368
[4]孙丽萍,王进欣,康淑荷,等.萼果香薷精油的化学成分[J].西北师范大学学报,2000.36(2):48-49
[5]谢亚雄,张占旺.木香薷挥发油化学成分的研究[J].质谱学报,1988.19(2):70-74
[6]曾虹燕,蒋丽娟,唐艳林,等.超临界CO2萃取石香薷挥发油工艺的研究[J],天然产物研究与开发.2003.15(1):51-54
[7]Masada Y.Analysis of essential oil by gas chromatography and mass spectrometry [M].New Yok:JohnWiley and sons.Inc.1976.5
[1]魏莲.γ-亚麻酸的研究进展[J].青海大学学报,2002,20(3):13-16.
[2]吴萍.月见草油的药效学研究概况[J].中国中医基础医学杂志,2001,7(10):75-77
[3]王北婴,王跃生,王焕奎,等.我国中药制药工业亟需推广的高新技术[J].中药现代化,2000,2(2):18-23
[1]张若梅.油脂抗氧化稳定性测试[J].粮油食品科技,2001,9(4):13-14
[2]王玉.海鞘油脂的提取及其氧化稳定性研究[J].中国海洋大学,2005,5:25-26
[3]赵贵兴.维生素E抗油脂氧化的功能[J].黑龙江农业科学,2003,(1):45-48
[4]傅冬和,刘仲华,黄建安,等.儿茶素在食用植物油中的抗氧化应用效果[J].茶叶科学,2005(1):61
[5]王璋,许时婴,汤坚.食品化学[M]北京:中国轻工业出版社,2006,109-111
[6]陈杭君,毛金林,陈文煊,等.富含油脂食品的抗氧化研究现状[J].浙江农业学报,2006,(3):335-337.
[7]Trans determination of edible oils by Fourier Transorm near infrared spectroscopy [J].Jmm.Oil chem.Soc,2000,77(10):1061-1067
[8]肇立春.浅谈食用油脂的氧化及其测定[J].粮食与食品工业,2003,(1):17-18
[9]马文平,纳鹏,蔡同,等.沙篙籽油的氧化稳定性研究[J].食品科学,2006,20(11):59-62
[10]龚钢明,王化田,荣绍丰,等.红景天超临界CO_2提取物抗氧化作用研究[J].食品工业,2007(4):13-15
[11]苏望懿主编.油脂加工艺学[M].武汉:湖北科学技术出版社,1997,107-108
[12]何东华.油脂精炼与加工艺学[M]北京:化学工业出版社,2005,213-214
[13]Hana Sobotova etal.Antioxidative activity of selected fruits and vegetables[J].Section Cellular and Molecular Biology.Biologia,Bratislava,2006,V3 279-284
[14]闫峰,常丽新,王莉.不同加热温度和时间对3种植物油品质的影响[J].安徽农业科学学,2007,35(20):67-69
[15]王毕妮,张富新,李建科.松籽油的抗氧化稳定性研究[J].食品工业科技,2005(7):96-98
[16]李全宏.植物油脂制品安全生产与品质控制[M].北京:化学工业出社,2005,3-4
[17]徐幸莲,彭增起,邓尚贵.食品原料学[M]北京:中国计量出版社,2006,95-98
[18]余佶,张永康,麻成金,等.抗氧化剂对猕猴桃籽油抗氧化性能的影响[J].吉首大学学报(自然科学版),2007(3):112-116
[19]麻成金,黄群,欧阳玉祝,等.抗氧化剂对杜仲籽油抗氧化性能影响的研究[J].食品科学,2006(27):50-53
[20]卢艳杰.油脂检测技术[M]北京:化学工业出版社,2004,55-57
[21]余裕娟,黄华军,徐惠金.油脂过氧化值的检测结果分析[J].职业与健康,2003,19(3):40-41
[22]马长伟,曾名勇.食品工艺学导论[M].北京:中国农业大学出版社,2002,262
[1]刘玉兰主编.油脂制取与加工工艺学.北京:科学技术出版社,2003
[2]郑诗超,陈宗道,阚健全.油脂天然抗氧化剂研究进展.粮食与油脂,2003,(6):43-44
[3]彭阳生编著.植物油脂加工实用技术.北京:金盾出版社,2003
[4]韩国麒编著.油脂化学.郑州:河南科学技术出版社,1995
[5]凌关庭.食品抗氧化剂及其进展(I).粮食与油脂,2000,(6):45-48
[6]凌关庭.食品抗氧化剂及其进展(III).粮食与油脂,2000,(8):45-47
[7]韩丽华,王丽红.大豆异黄酮抗氧化性的研究.中国油脂,2001,26(8):41-44
[8]王绍美,赵讲芬.茶多酚对食用油脂抗氧化作用的研究.西部粮油科技,1997,22(2):44-46
[9]闫峰,常丽新,王莉.不同加热温度和时间对3种植物油品质的影响[J].安徽农业科学学,2007,35(20):60-62
[10]王毕妮,张富新,李建科.松籽油的抗氧化稳定性研究[J].食品工业科技,2005(7):96-98
[11]赵声兰,李涛,蔡绍芬,等.几种抗氧化剂对核桃油抗氧化性能的研究[J].食品科学,2002,23(2):135-138
[12]王毕妮,张富新,等.松籽油的抗氧化稳定性研究[J].研究与探讨,2005(7):96-98
[13]余佶,张永康,麻成金,等.抗氧化剂对猕猴桃籽油抗氧化性能的影响[J].吉首大学学报(自然科学版),2007(3):112-116
[14]赵声兰,李涛,蔡绍芬,等.几种抗氧化剂对核桃油抗氧化性能的研究[J].食品科学,2002,23(2):135-138
[15]王毕妮,张富新,等.松籽油的抗氧化稳定性研究[J].研究与探讨,2005(7):96-98
[16]魏香,陈朝银,蔡嵩,等.三种食品抗氧化剂对核桃油抗氧化作用的影响[J].中国油脂,2000,25(6):134-136
[17]王兴国,裘爱泳,史小华,等.抗坏血酸棕榈酸酯在不同油品中的抗氧化性能研究[J].中国油脂,2000,25(3):52-55
[18]Hudson B J F.Food antioxidands.New York:Marcel Dekker Inc.1996.
[19]Dziezak JK.Microencapsulation and encapsulated ingredients[J].Food Technnl,1988(4):136-151
[1]Chen Q X,Chan Laureen L Y.Bitter melon(Momordica charantia L.) reduces adiposity,lowers serum insulin and normalizes glucose tolerance in rats fed a high fat diet[J].J Nutri,2003,133(4):1088-1093.
[2]Miura T,Itoh Y,Iwanmoto N,et al.Suppressive activity of the fruit of Momordica with exercise on blood glucose in type 2-diabetic mice[J].Biol&Pharmac Bulletin,2004,27:248-250.
[3]Tongia A,Tongia S,Dave M,et al.Phytochemical determination and extraction of Momordica Charantia fruit and its hypoglycemic potentiation of oral hypoglycemic drugs in diabetes mellitus(NIDDM)[J].J Physiol & Pharm,2004,48:241-244.
[4]Issemarm J I,Green S.Activation of a member of the steroid hormone receptor super-family by pero-xisome pro-liferator activate dreceptor[J].Nature,1990,347:645-650.
[5]廖晨钟,邓沱,山松,等.以PPAR为靶标的抗2型糖尿病药物研发策略[J].中国新药杂志,2003,12(4):980-983.
[6]Keith J W,Richard A M,Michael W.Recent advances in discovery technologies[J].Neurotransmissions,1997,5(3):24-28.
[1]鲍鲁生.食品工业中应用的微胶囊技术[J].食品科学,1999,20(9):75-78
[2]黄英雄,华聘聘.用于油脂微胶囊化一些壁材[J].粮食与油脂,2002,30(1):60-62
[3]宋建,陈磊,李效军.微胶囊化技术及应用北京:化学工业出版社,2001,9.
[4]钱列生,芮汉明.食品微胶囊技术.中山大学学报.2007,26(6):45-47
[5]王海燕,马一长伟,麻成金.湖南腊肉在加工过程中挥发性成分的组成和变化.第五届中国肉类大会论文集,2006,11
[6]牛广财,朱丹,李志江,等.南瓜籽油微胶囊化的研究[J].食品科学,2007,38(9):60-63
[7]余估,张永康,麻成金,等.抗氧化剂对称猴桃籽油抗氧化性能的影响.吉首大学学报(自然科学版),2007,22(3):22-25
[8]欧阳林,麻成金,黄群,等.混合溶剂浸提茶叶籽油的研究[J].食品与发酵,2007,25(4):32-35
[9]鲍鲁生,食品工业中应用的微胶囊技术(续)[J].北京:食品科学,1999,18(10):52-54
[10]高福成,王海鸥.现代食品工程高新技术.北京:中国轻工业出版社,1997,5
[11]杨芙莲,夏银,邓宇凤.荞麦类黄酮微胶囊化的研究[J].食品科技,2007,25(2):28-30
[12]姚茂君,刘飞.猕猴桃籽油的微胶囊化研究[J].生产与科研经验,2006,15(10):12-14
[13]孙厚良.喷雾干燥法制备微胶囊工艺研究[J].化工时刊,2005.19(10):16-20
[14]李书国,薛文通,李雪梅,等.乙基纤维素微胶囊化Vc工艺参数及其活性保护的研究[J].食品工业科技,2005,26(5):143-145
[15]张根旺.油料中功能性成分的研究开发与油脂工程技术的发展[J].中国工程科学.2000,2(3):50-53
[16]K.S.Soppimath,A.R.Kulkarni,T.M.Aminabhavi.Cellulose acetate prepared by o/w emulsification and solvent evaporation method.Journal of Mirrnancancnlatinn.2001.18(6):811-817
[17]Er-Tong Chen,Ting Wang,Mi Peng,Bin Zhou,Bo Xu.Research on the Microcapsule Production by High Voltage Pulsing Method.Proceedings of the 2005IEEE Engineering in Medicine and Biology 27th Annual ConferenceShanghai,China,September.2005.26(4):234-238
[1]徐淑云,卞如镰,药理实验方法学(第三版)北京:人民卫生出版社,2000,1784-1789
[2]陈奇,中药药理研究方法学北京:人民卫生出版社,1993,878-879
[2].Huang LQ(黄璐琦) 1995.Study on the systematics of Trichosanthes L(Cucurbitaceae).The Doctor Degree of Medical Science Department of Beijing University
[3].Kurz,S.Contribution towards alhowlcdge of Burmese flora.Journ.AS.Soc Beng 1877,46(2)97-99
[4].Yue C-X(乐崇熙),Chen J-R(诚静容).Apreliminary study on the Chinese medicinal species of genus Trichosanthes L Acta Phytotaxonomica Sinica(植物分类学报) 1974,12:415-446
[5].Yue C-X(乐崇熙),Chen J-R(诚静容).The Chinese medicinal species of the genus Trichosanthes.L Actr Phytotaxonomica Sinica(植物分类学报) 1980,18:333-352
[6].黄璐琦,桔蒌属(Trichosanthes)药用植物的细胞学及根的化学鉴别研究,中国中医研究院硕士学位论文,1992
[7].Yue C-X(乐崇熙),Zhang Y-L(张玉龙)1986 Studies on the pollen motholo of chinese Trichosathes.Bulletin of Botanical Research(植物研究) 6:21-35
[8].Huang L-Q(黄璐琦),Yue C-X(乐崇熙),Li M-X(李懋学)Chromosome numbers of 8 species in Trichosanthes L Acta Botanica Yunnanica(云南植物研究) 199416:95-96
[9].Bhaduri P N Cytogenetical investigations in some chromosomes as a physical basis of speciation.Joural Genetics 1947,48:237-256
[10].Rangaswami K,Tatuno,Yoshida Scx chromosomes of Trichosanthes palmate Roxb Proceedings of Indian Science,1949,3:137
[11].Darlington CD,1955,Chromosome altas of flowering plants.London G Aeeen Press.
[12].Thakur GK Cytology of a tetraploid Trichosanthes bracteata(Lam).Voigt from Bihar Journal Cytologic Genetics 1976,11:47-50
[13].Singh A K Cytological studies in Trichosanthes L Journal Cytogenetics,1979,14:50-57
[14].Pandcy V Thompson W.B Chromosome in Trichosanthes lepiniana and Cogn.Current Science 1986,55:159
[15].E.B Byjib,1960 An introduction to historical plant geography Beijing:Science Press
[16]黄璐琦,杨滨,乐崇熙.括蒌属药用植物资源调查.中国中药杂志,1995,20(4):195-197
[17]陈书坤.中国括蒌属的新分类群.植物研究,1985,5(2):113-121
[18]蒌之岑,秦波.常用中药材品种整理和质量研究.北京:北京医科大学、中国协 和医科大学联合出版社,1996,585
[19]陈胜发,黄时伟,王昌利.括蒌属植物化学成分的研究进展.中成药,2006,28(8):1187-1192
[20]巢志茂,刘静明.湖北括萎果皮挥发油化学成分的研究.中国药学杂志,1996,31(3):140-141
[21]霍启录,陈振德,许重远.双边括蒌种子油含量及其脂肪酸的测定.华西药学杂志,2002,17(5):351-352
[22]巢志茂,刘静明.双边括蒌中括蒌碱的结构研究.药学学报,1995,30(7):517-520
[23]巢志茂,刘静明.双边括萎皮挥发油的化学成分研究.中国中药杂志,21(6):357-384
[24]时岩鹏,姚庆强,刘拥军.括蒌化学成分的研究及其菠菜昌醇的含量测定.中草药,2002,33(1):14-16
[25]巢志茂,何波.括蒌果实的化学成分研究.中国中药杂志,1999,24(10):612-613
[26]巢志茂,何波,张颖.括蒌种子中不皂化类脂的化学成分研究.中国药学杂志,2000,35(11):131-135
[27]黄民权.括蒌属植物种子中特殊脂肪酸成分的研究.植物研究,1986,6(1):79-83
[28]胡合妓,王鸿,潘远江.GC-MS法测定余杭括萎根块的挥发油成分.林产化学与工业,2005,25(1):109-111
[29]何祥久,印峰,姚新生,等.括蒌属植物化学成分.国外医药·植物药分册,2002,17(1):11-13
[30]ItohT,YashidaK,TarnuraT,etal.3thyl-sterolsin the roots of Triehosanthes joniea,chemistiy,1982,21(3):727
[31]AkihisaT,TamuraT,MatsumotoT.Karounidiol and its 3benzoate:novel Pentaey clietri terpenes from Trichosan the skirilowi ray molecular settireof karouni diol diacetate.J Chem Soc Perkin Transl Org Chem,1988,(3):439
[32]AkihisaT,Kokke WCMC Kimuraet first uaturall yal.Isokarounidio the occurring triterpene with6,8-conjugateddienesystem.iodine mediated dedrogenation and isomeri Zationof its diacetate.JO Chem,1993,58(7):1959
[33]李丰.括蒌籽中一种新的具有蛋白质生物合成抑制活性的多肤-Trichokirin-S1的分离、纯化和性质.生物化学与生物物理学报,2003,35(9):841-845
[34]陈毓荃,吕志强,吴伸.括蒌籽核糖体失活蛋白(TCK)与天花粉蛋白(TCS)'胜质比较研究.西北植物学报,1998,18(3):423
[35]邱明华,陈书坤,陈剑超,等.葫芦科化学分类学.应用与环境生物学报,2005,11(6):673-68
[36]HilditehTP,etal.Fatty acids and glycerides of solid seed fats VIII:the seed fat of Hodgsonia eapnio carpa.JSoeChemhid,1939,58:27-28
[37]樊娟,冯宝树,邱明华,等.臭瓜根的化学成分.云南植物研究,1988,10(4):475-479.
[38]Dubo is MA,BauerR,Cagiotti MR and Wa gner H.Foeti dissimoside A,anew3,2-bidesmosi dictriter penoid saponin,and eueur bit aeins from Cucur bita foeti dissima.Phyto-chemistry,1988,27(3):881-885
[39]贝伟剑.瓜蒌建白汤药理研究概况.广西中医药,1998,18(3):423
[40]黄美兰,贝伟剑.大子括蒌和括蒌的药理作用比较.广东药学,2000,10(1):47
[41]AkihisaT,hsukawaK,KimuraY,etal.karounidiol.括蒌中的一种新的三萜.国外医学中医中药分册,1994,16(1):41
[42]邵春丽,王世久,王进.瓜蒌皮抗缺氧作用的研究.沈阳药科大学学报,1998,1998,15(1):38-31
[43]凌宏,鲁翔,董传仁.瓜蒌、心得安对血小板体外聚集和TXAZ合成的影响.湖北医学院学报,1988,9(2):138
[44]汪酞,金善炜.2000,天花粉蛋白.北京:科学出版社
[45]玄焕开,刘普,王菊.葫芦素的研究概况.齐鲁药事,2005,24(12):737-739
[46]秦林,高伟良.瓜蒌对子宫颈癌细胞和巨噬细胞的影响.山东中医学院学报,1995,19(6):414-417
[47]卢兆华.抗癌植物-括蒌.福建热作科技,1995,20(1):28-29
[48]贝伟剑,宋黎纳,赵一大子括蒌的药理作用.时珍国医国药研究,2002,6(2):20-22
[49]曹焕生,徐明芳,李定坚.大苞括蒌的药用价值分析.药物生物技术,2002,9(5):292-296
[50]彭桂福,李彩琴.食用括蒌的生育特性及栽培技术.中国西瓜甜瓜,2003,(4):44-40
[51]任静,何家庆.括蒌子资源的利用及其价值.中国野生植物资源,2000,20(6):21-22
[52]洪金祥,赵长江.多用途多效能的垂直绿化材料-括蒌.林业科技通迅,1994,(11):42
[53]杨廷祯,高敬东,杨明霞.括蒌的经济价值及栽培技术要点。甘肃农业科技,2003,(10):48-50.
[54]郎进宝,张贤朝,王珍儿.括蒌的特性及其丌发利用.上海农业科技,2004,(2):91
[55].Wang YQ LaiBS Yan XL,et al Analys is the composition of fatty acid from perilla frutiscens seeds and linseed with GC/MS[J]Chin I Biochem Pham(中国生化药物志 ),1999,20(2):62-64
[56].Yin L J,Wang Y Zu Y G Purification of linolenic acid from fruit oil of Camptotheca acuminata[J].Chin Trad it Herb Drugs(中草药),2002,33(1):22-23
[57].Ezaki O Takahashi M Shigem atsu T etal Long+trem effects of dietary alpha linolenic acid from perilla oil on serum fatty acids corn position and on the fisk factors of coronary heart disease in Japanese elderly subjects[J]Nutr Scivitaminol.1999,45(6):759-772
[58].Renaus S Lanzmann-petithory D Dietary fats and coronary heart disease pathogenesis[J].Curr A theroscler Rep,2002,4(6):419-424
[59].Ding G L,Hu DY,Wang B Suppression of transient out ward current by linolenic acid in atria myocytes from patienst with heart failure[J].Bullmed Res(医药研究通讯) 2000,29(6):8-9
[60].Lin H,Shen JQ,Xin D etal Effects of flaxseed on blood lipids on rats[J]I Capita LUniu Med Sci(首都医科大学学报),2001,22(2):104-106
[61].Chen J,Stavro P M,Thompson L U,Dietary flaxseed inhibits human breast cancer grow thand metastasis and down regulated expression of insulin like growth factor and epidermal grow th factor receptor[J].Nutr Cancer,2002,43(2):187-192
[62].Xie L T,Huang JQ Cytotoxic action and effect of unsaturated fatty acids and their selenidses on DNA synthes is in BEL-7402 human hepatocarcinom a cell[J]I Chin Tumour Clin(中国肿瘤临床),1998,25(7):501-504
[63].Lee J Y,Kim Y S,Shin D H.Anti microbial synerg is ticeffect of linolenic acid and monoglyceride against Bacillus cereus and Staphylococcus aureus[J].Agric Food Chem,2002,50(7):2193-2199
[64]. Kakus, Yunokis, Ohkura K etal Interactions of dietary fats and proteins on fatty acid composition of immune cells and LTB4 production by peritoneal exudates cells of rats [J] Biosci B iotechnol Biochem, 2001,65(2):315-321
[65]. Suresh Y Das U N Long-chain polyunsaturated fatty acids and chem. Ically induced diabetes mellitus. Effect of o mega-3 fatty acids [J] Nutrition, 2003,19(3):213-228
[66]. Ikem oto A Fukuma A, Fujii Y, etal Lysosomal enzyme activities are decreased in the retina and their circadian rhythms are different from those in the pineal gland of rats fed and alpha linolenic acid restricted diet [J] Nutrition 2000,130(12):3059-3062
[67]. Xu ZH Shao Y F Effect of linolenic acid on rat's behavior, retina, and liver, brain fatty acid composition [J]. Chin Public Health(中国公共卫生),2002,18 (3): 301-304
[68]. Umezawa M Kogishik, Tojo H etal High-linoleate and high-alpha-linolenate diets affect learning ability and natural behavior in SAMRL mice [J] Nutrition, 1999, 129(2):431-437
[69]. Zhang H M, Liu F Z, Dai L M ,Purity method of linolenic acid on the theory of urea adduction fractionation (I)-study on the noticeable factors affecting purification behavior of linolenic acid [J] Chin Lipid,2001,26(2):41-44
[70]. Zhu D. Wang S C Guo Y etal Purification of linolenic acid from Perilla frutiscens oil [J] Chin Tradit Herb Drugs 1997,28(6):242-243
[71]. Wang W, LiM J, Liu J H, etal Research on the quality standardization criterion on perilla frutiscens. Preparation and identification ethanol linolenic ester [J]. Chin Tradit Herb Drugs, 1999,30(4):267-268
[72]. Szentm ihalyik, Vinkler P, Lakatos B etal Rose hip(Rosa canina L)Oil obtained from waste hip seeds by different extraction methods [J]. Bioresour Technol, 2002,82(2):195-201
[73]. Shen X J, Lai B S, Chen Z T, etal, Effec of time of super critical fluid extraction on quality of fatty acids in plant medicines oil [J] Chin I pharm 2002,33(11):533-535
[74]. Chen Y Yang J C Studies on supercritical carbon dioxide extraction of linseed iol[J]. Nat Prod ResDEV,2002,13(3):14-19
[75]. Fu H Gan A Y Preparation of polyunsaturated fatty acid from fish oil with molecular distillation[J]I Wuxi univ Light Ind 200,21(6):617-621
[75]汪言满,陈达美,黎碧娜.超临界CO_2流体萃取技术在食品工业中的新应用[J].广州化工,20000,28(4):44-45
[76]彭阳生编著.植物油脂加工实用技术.北京:金盾出版社,2003
[78]韩国麒编著.油脂化学.郑州:河南科学技术出版社,1995
[1]沈晓京,赖炳森,陈镇童,等.超临界萃取时间对植物药油脂产率和脂肪酸质量的影响[J].中国医药工业杂志,2002,33(11):533-535
[2]Alkiom,Gonzalezc,Janttim,etal.Purl flcation of poly unsaturated fatty acid sesters from Tuna with superoritical fluid[J].Chromatography.JAOCS,2000,77(3):315-321
[3]李章万,周同惠.香薷挥发油成分的研究[J].药学学报,1983.18(5):363-368
[4]孙丽萍,王进欣,康淑荷,等.萼果香薷精油的化学成分[J].西北师范大学学报,2000.36(2):48-49
[5]谢亚雄,张占旺.木香薷挥发油化学成分的研究[J].质谱学报,1988.19(2):70-74
[6]曾虹燕,蒋丽娟,唐艳林,等.超临界CO2萃取石香薷挥发油工艺的研究[J],天然产物研究与开发.2003.15(1):51-54
[7]Masada Y.Analysis of essential oil by gas chromatography and mass spectrometry [M].New Yok:JohnWiley and sons.Inc.1976.5
[1]魏莲.γ-亚麻酸的研究进展[J].青海大学学报,2002,20(3):13-16.
[2]吴萍.月见草油的药效学研究概况[J].中国中医基础医学杂志,2001,7(10):75-77
[3]王北婴,王跃生,王焕奎,等.我国中药制药工业亟需推广的高新技术[J].中药现代化,2000,2(2):18-23
[1]张若梅.油脂抗氧化稳定性测试[J].粮油食品科技,2001,9(4):13-14
[2]王玉.海鞘油脂的提取及其氧化稳定性研究[J].中国海洋大学,2005,5:25-26
[3]赵贵兴.维生素E抗油脂氧化的功能[J].黑龙江农业科学,2003,(1):45-48
[4]傅冬和,刘仲华,黄建安,等.儿茶素在食用植物油中的抗氧化应用效果[J].茶叶科学,2005(1):61
[5]王璋,许时婴,汤坚.食品化学[M]北京:中国轻工业出版社,2006,109-111
[6]陈杭君,毛金林,陈文煊,等.富含油脂食品的抗氧化研究现状[J].浙江农业学报,2006,(3):335-337.
[7]Trans determination of edible oils by Fourier Transorm near infrared spectroscopy [J].Jmm.Oil chem.Soc,2000,77(10):1061-1067
[8]肇立春.浅谈食用油脂的氧化及其测定[J].粮食与食品工业,2003,(1):17-18
[9]马文平,纳鹏,蔡同,等.沙篙籽油的氧化稳定性研究[J].食品科学,2006,20(11):59-62
[10]龚钢明,王化田,荣绍丰,等.红景天超临界CO_2提取物抗氧化作用研究[J].食品工业,2007(4):13-15
[11]苏望懿主编.油脂加工艺学[M].武汉:湖北科学技术出版社,1997,107-108
[12]何东华.油脂精炼与加工艺学[M]北京:化学工业出版社,2005,213-214
[13]Hana Sobotova etal.Antioxidative activity of selected fruits and vegetables[J].Section Cellular and Molecular Biology.Biologia,Bratislava,2006,V3 279-284
[14]闫峰,常丽新,王莉.不同加热温度和时间对3种植物油品质的影响[J].安徽农业科学学,2007,35(20):67-69
[15]王毕妮,张富新,李建科.松籽油的抗氧化稳定性研究[J].食品工业科技,2005(7):96-98
[16]李全宏.植物油脂制品安全生产与品质控制[M].北京:化学工业出社,2005,3-4
[17]徐幸莲,彭增起,邓尚贵.食品原料学[M]北京:中国计量出版社,2006,95-98
[18]余佶,张永康,麻成金,等.抗氧化剂对猕猴桃籽油抗氧化性能的影响[J].吉首大学学报(自然科学版),2007(3):112-116
[19]麻成金,黄群,欧阳玉祝,等.抗氧化剂对杜仲籽油抗氧化性能影响的研究[J].食品科学,2006(27):50-53
[20]卢艳杰.油脂检测技术[M]北京:化学工业出版社,2004,55-57
[21]余裕娟,黄华军,徐惠金.油脂过氧化值的检测结果分析[J].职业与健康,2003,19(3):40-41
[22]马长伟,曾名勇.食品工艺学导论[M].北京:中国农业大学出版社,2002,262
[1]刘玉兰主编.油脂制取与加工工艺学.北京:科学技术出版社,2003
[2]郑诗超,陈宗道,阚健全.油脂天然抗氧化剂研究进展.粮食与油脂,2003,(6):43-44
[3]彭阳生编著.植物油脂加工实用技术.北京:金盾出版社,2003
[4]韩国麒编著.油脂化学.郑州:河南科学技术出版社,1995
[5]凌关庭.食品抗氧化剂及其进展(I).粮食与油脂,2000,(6):45-48
[6]凌关庭.食品抗氧化剂及其进展(III).粮食与油脂,2000,(8):45-47
[7]韩丽华,王丽红.大豆异黄酮抗氧化性的研究.中国油脂,2001,26(8):41-44
[8]王绍美,赵讲芬.茶多酚对食用油脂抗氧化作用的研究.西部粮油科技,1997,22(2):44-46
[9]闫峰,常丽新,王莉.不同加热温度和时间对3种植物油品质的影响[J].安徽农业科学学,2007,35(20):60-62
[10]王毕妮,张富新,李建科.松籽油的抗氧化稳定性研究[J].食品工业科技,2005(7):96-98
[11]赵声兰,李涛,蔡绍芬,等.几种抗氧化剂对核桃油抗氧化性能的研究[J].食品科学,2002,23(2):135-138
[12]王毕妮,张富新,等.松籽油的抗氧化稳定性研究[J].研究与探讨,2005(7):96-98
[13]余佶,张永康,麻成金,等.抗氧化剂对猕猴桃籽油抗氧化性能的影响[J].吉首大学学报(自然科学版),2007(3):112-116
[14]赵声兰,李涛,蔡绍芬,等.几种抗氧化剂对核桃油抗氧化性能的研究[J].食品科学,2002,23(2):135-138
[15]王毕妮,张富新,等.松籽油的抗氧化稳定性研究[J].研究与探讨,2005(7):96-98
[16]魏香,陈朝银,蔡嵩,等.三种食品抗氧化剂对核桃油抗氧化作用的影响[J].中国油脂,2000,25(6):134-136
[17]王兴国,裘爱泳,史小华,等.抗坏血酸棕榈酸酯在不同油品中的抗氧化性能研究[J].中国油脂,2000,25(3):52-55
[18]Hudson B J F.Food antioxidands.New York:Marcel Dekker Inc.1996.
[19]Dziezak JK.Microencapsulation and encapsulated ingredients[J].Food Technnl,1988(4):136-151
[1]Chen Q X,Chan Laureen L Y.Bitter melon(Momordica charantia L.) reduces adiposity,lowers serum insulin and normalizes glucose tolerance in rats fed a high fat diet[J].J Nutri,2003,133(4):1088-1093.
[2]Miura T,Itoh Y,Iwanmoto N,et al.Suppressive activity of the fruit of Momordica with exercise on blood glucose in type 2-diabetic mice[J].Biol&Pharmac Bulletin,2004,27:248-250.
[3]Tongia A,Tongia S,Dave M,et al.Phytochemical determination and extraction of Momordica Charantia fruit and its hypoglycemic potentiation of oral hypoglycemic drugs in diabetes mellitus(NIDDM)[J].J Physiol & Pharm,2004,48:241-244.
[4]Issemarm J I,Green S.Activation of a member of the steroid hormone receptor super-family by pero-xisome pro-liferator activate dreceptor[J].Nature,1990,347:645-650.
[5]廖晨钟,邓沱,山松,等.以PPAR为靶标的抗2型糖尿病药物研发策略[J].中国新药杂志,2003,12(4):980-983.
[6]Keith J W,Richard A M,Michael W.Recent advances in discovery technologies[J].Neurotransmissions,1997,5(3):24-28.
[1]鲍鲁生.食品工业中应用的微胶囊技术[J].食品科学,1999,20(9):75-78
[2]黄英雄,华聘聘.用于油脂微胶囊化一些壁材[J].粮食与油脂,2002,30(1):60-62
[3]宋建,陈磊,李效军.微胶囊化技术及应用北京:化学工业出版社,2001,9.
[4]钱列生,芮汉明.食品微胶囊技术.中山大学学报.2007,26(6):45-47
[5]王海燕,马一长伟,麻成金.湖南腊肉在加工过程中挥发性成分的组成和变化.第五届中国肉类大会论文集,2006,11
[6]牛广财,朱丹,李志江,等.南瓜籽油微胶囊化的研究[J].食品科学,2007,38(9):60-63
[7]余估,张永康,麻成金,等.抗氧化剂对称猴桃籽油抗氧化性能的影响.吉首大学学报(自然科学版),2007,22(3):22-25
[8]欧阳林,麻成金,黄群,等.混合溶剂浸提茶叶籽油的研究[J].食品与发酵,2007,25(4):32-35
[9]鲍鲁生,食品工业中应用的微胶囊技术(续)[J].北京:食品科学,1999,18(10):52-54
[10]高福成,王海鸥.现代食品工程高新技术.北京:中国轻工业出版社,1997,5
[11]杨芙莲,夏银,邓宇凤.荞麦类黄酮微胶囊化的研究[J].食品科技,2007,25(2):28-30
[12]姚茂君,刘飞.猕猴桃籽油的微胶囊化研究[J].生产与科研经验,2006,15(10):12-14
[13]孙厚良.喷雾干燥法制备微胶囊工艺研究[J].化工时刊,2005.19(10):16-20
[14]李书国,薛文通,李雪梅,等.乙基纤维素微胶囊化Vc工艺参数及其活性保护的研究[J].食品工业科技,2005,26(5):143-145
[15]张根旺.油料中功能性成分的研究开发与油脂工程技术的发展[J].中国工程科学.2000,2(3):50-53
[16]K.S.Soppimath,A.R.Kulkarni,T.M.Aminabhavi.Cellulose acetate prepared by o/w emulsification and solvent evaporation method.Journal of Mirrnancancnlatinn.2001.18(6):811-817
[17]Er-Tong Chen,Ting Wang,Mi Peng,Bin Zhou,Bo Xu.Research on the Microcapsule Production by High Voltage Pulsing Method.Proceedings of the 2005IEEE Engineering in Medicine and Biology 27th Annual ConferenceShanghai,China,September.2005.26(4):234-238
[1]徐淑云,卞如镰,药理实验方法学(第三版)北京:人民卫生出版社,2000,1784-1789
[2]陈奇,中药药理研究方法学北京:人民卫生出版社,1993,878-879