大针茅质膜水通道蛋白基因（SgPIPs）全长cDNA序列的克隆及序列分析
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摘要
水通道蛋白不但为水分跨细胞膜的运输提供了一条选择性通道,而且水通道蛋白在植物种子萌发、细胞伸长、气孔运动、受精及植物逆境应答中起重要作用。研究以亚洲中部特有的典型草原种大针茅(Stipa grandis P.Smirn)为材料,通过反转录聚合酶链式反应(RT-PCR)和快速分离cDNA末端技术(RACE),克隆得到大针茅的质膜水通道蛋白基因(SgPIPs)的全长cDNA序列,并对其进行生物信息学分析。主要结果如下:
共获得SgPIPs的9个中间片段cDNA序列,22个3′端cDNA序列,9个5′端cDNA序列。克隆到3个SgPIPs的cDNA全长序列,分别为SgPIP1-1、SgPIP1-2和SgPIP1-3。
SgPIP1-1全长cDNA序列为991bp,包含15bp的5′非翻译区,870bp的开放阅读框,106bp的3′非翻译区,编码289个氨基酸。SgPIP1-2全长cDNA序列为1248bp,包含15bp的5′非翻译区,870bp的开放阅读框,363bp的完整3′非翻译区,编码289个氨基酸;SgPIP1-3全长cDNA序列为1068bp,包含58bp的5′非翻译区,867bp的开放阅读框,143bp的完整3′非翻译区,编码288个氨基酸。
SgPIP1-1、SgPIP1-2、SgPIP1-3推导氨基酸序列表明:它们不但具有MIP家族的信号序列SGXHXNPAVT,而且具有质膜水通道蛋白的特征信号序列GGGANXXXXGY和TGI/TNPARSI/FGAAI/VI/VF/YN。同时具有水通道蛋白典型的6个跨膜区和两个NPA盒单元。
SgPIP1-1、SgPIP1-2、SgPIP1-3的核苷酸序列和禾本科物种构建的系统进化树表明:SgPIP1-1和SgPIP1-3进化关系最近,之后与玉米、甘蔗、水稻聚为一类。SgPIP1-2与大麦、小麦的进化关系最近。SgPIP1-1、SgPIP1-2、SgPIP1-3推导的氨基酸序列和大麦PIPs基因家族的PIP1和PIP2构建系统进化树,结果表明:SgPIP1-1、SgPIP1-2、SgPIP1-3均为PIP1类基因亚家族成员。
Aquaporin represent an important selective pathway for water to move across cellular membrane, and involved in seed germination,cell elongation,stomatal movement,fertilization, and even some plant aquaporin that play an important role in response to stress. A typical grassland plants, Stipa grandis P.Smirn, growing in the steppe region of central Asia was collected and cultured. Full-length cDNA sequence of plasma membrane aquaporin genes from Stipa grandis was acquired by RT-PCR and RACE technology. Their structure of sequence of nucleotide and amino acid were analyzed by bioinformatics software. The mainly results as follows:
The experiment obtained a total of nine core fragment cDNA sequences of SgPIPs, twenty-two 3′end cDNA sequences of SgPIPs, nine 5′end cDNA sequences of SgPIPs. The experiment cloned three full-length cDNA sequences of SgPIPs, named SgPIP1-1, SgPIP1-2 and SgPIP1-3.
SgPIP1-1 full-length cDNA sequence consists of 991bp, which includes the length of 15bp of the 5′untranslated region, followed by 870bp of the open reading frame encoding 289 amino acids, and finally a 106bp of 3′untranslated region. SgPIP1-2 full-length cDNA sequence consists of 1248bp, which includes the length of 15bp of the 5′untranslated region, followed by 870bp of the open reading frame encoding 289 amino acids, and finally a 363bp of 3′untranslated region. SgPIP1-3 full-length cDNA sequence consists of 1068bp, which includes the length of 58bp of the 5′untranslated region, followed by 867bp of the open reading frame encoding 288 amino acids, and finally a 143bp of 3′untranslated region.
SgPIP1-1, SgPIP1-2, and SgPIP1-3 amino acid sequence analysis showed that their not only have the MIP family of signal sequence SGXHXNPAVT, but also the characteristics of plasma membrane water channel protein signal sequence GGGANXXXXGY and TGI/TNPARSI/FGAAI/VI/VF/YN. Besides their have the typical six transmembrane domains and two NPA units.
phylogenetic tree constructed by nucleotide sequences Between SgPIP1-1, SgPIP1-2, and SgPIP1-3 and gramineous species which are very closer in relationship showed that SgPIP1-1 and SgPIP1-3 the most closest in evolution, and then with Zea mays、Saccharum officinarum、Oryza sative clustered together. SgPIP1-2, Hordeum vulgare, and Triticum aestivum have the closest relationship in evolution. Phylogenetic tree constructed by amino acids sequences between SgPIP1-1, SgPIP1-2, and SgPIP1-3 and all PIPs of barley showed that SgPIP1-1, SgPIP1-2, and SgPIP1-3 were PIP1 subfamily members.
共获得SgPIPs的9个中间片段cDNA序列,22个3′端cDNA序列,9个5′端cDNA序列。克隆到3个SgPIPs的cDNA全长序列,分别为SgPIP1-1、SgPIP1-2和SgPIP1-3。
SgPIP1-1全长cDNA序列为991bp,包含15bp的5′非翻译区,870bp的开放阅读框,106bp的3′非翻译区,编码289个氨基酸。SgPIP1-2全长cDNA序列为1248bp,包含15bp的5′非翻译区,870bp的开放阅读框,363bp的完整3′非翻译区,编码289个氨基酸;SgPIP1-3全长cDNA序列为1068bp,包含58bp的5′非翻译区,867bp的开放阅读框,143bp的完整3′非翻译区,编码288个氨基酸。
SgPIP1-1、SgPIP1-2、SgPIP1-3推导氨基酸序列表明:它们不但具有MIP家族的信号序列SGXHXNPAVT,而且具有质膜水通道蛋白的特征信号序列GGGANXXXXGY和TGI/TNPARSI/FGAAI/VI/VF/YN。同时具有水通道蛋白典型的6个跨膜区和两个NPA盒单元。
SgPIP1-1、SgPIP1-2、SgPIP1-3的核苷酸序列和禾本科物种构建的系统进化树表明:SgPIP1-1和SgPIP1-3进化关系最近,之后与玉米、甘蔗、水稻聚为一类。SgPIP1-2与大麦、小麦的进化关系最近。SgPIP1-1、SgPIP1-2、SgPIP1-3推导的氨基酸序列和大麦PIPs基因家族的PIP1和PIP2构建系统进化树,结果表明:SgPIP1-1、SgPIP1-2、SgPIP1-3均为PIP1类基因亚家族成员。
Aquaporin represent an important selective pathway for water to move across cellular membrane, and involved in seed germination,cell elongation,stomatal movement,fertilization, and even some plant aquaporin that play an important role in response to stress. A typical grassland plants, Stipa grandis P.Smirn, growing in the steppe region of central Asia was collected and cultured. Full-length cDNA sequence of plasma membrane aquaporin genes from Stipa grandis was acquired by RT-PCR and RACE technology. Their structure of sequence of nucleotide and amino acid were analyzed by bioinformatics software. The mainly results as follows:
The experiment obtained a total of nine core fragment cDNA sequences of SgPIPs, twenty-two 3′end cDNA sequences of SgPIPs, nine 5′end cDNA sequences of SgPIPs. The experiment cloned three full-length cDNA sequences of SgPIPs, named SgPIP1-1, SgPIP1-2 and SgPIP1-3.
SgPIP1-1 full-length cDNA sequence consists of 991bp, which includes the length of 15bp of the 5′untranslated region, followed by 870bp of the open reading frame encoding 289 amino acids, and finally a 106bp of 3′untranslated region. SgPIP1-2 full-length cDNA sequence consists of 1248bp, which includes the length of 15bp of the 5′untranslated region, followed by 870bp of the open reading frame encoding 289 amino acids, and finally a 363bp of 3′untranslated region. SgPIP1-3 full-length cDNA sequence consists of 1068bp, which includes the length of 58bp of the 5′untranslated region, followed by 867bp of the open reading frame encoding 288 amino acids, and finally a 143bp of 3′untranslated region.
SgPIP1-1, SgPIP1-2, and SgPIP1-3 amino acid sequence analysis showed that their not only have the MIP family of signal sequence SGXHXNPAVT, but also the characteristics of plasma membrane water channel protein signal sequence GGGANXXXXGY and TGI/TNPARSI/FGAAI/VI/VF/YN. Besides their have the typical six transmembrane domains and two NPA units.
phylogenetic tree constructed by nucleotide sequences Between SgPIP1-1, SgPIP1-2, and SgPIP1-3 and gramineous species which are very closer in relationship showed that SgPIP1-1 and SgPIP1-3 the most closest in evolution, and then with Zea mays、Saccharum officinarum、Oryza sative clustered together. SgPIP1-2, Hordeum vulgare, and Triticum aestivum have the closest relationship in evolution. Phylogenetic tree constructed by amino acids sequences between SgPIP1-1, SgPIP1-2, and SgPIP1-3 and all PIPs of barley showed that SgPIP1-1, SgPIP1-2, and SgPIP1-3 were PIP1 subfamily members.
引文
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