CSISV对肉仔鸡主要免疫器官的致病性及免疫的影响
摘要
鸡生长与免疫抑制综合征(CSIS)是近年来危害养鸡业的重要传染性疾病之一,其病原的致病性和对免疫的影响,是防治该病的主要依据。本研究在刘兴友教授前期研究证明CSIS为一种新的疾病的基础上,开展了CSISV对肉仔鸡主要免疫器官的致病性研究和对免疫功能的影响的研究。
将CSISV人工感染3日龄肉仔鸡(美国ROSS308),分别在感染后24、48、72、96、120、144h等采取脾脏、胸腺和法氏囊,测定其器官指数,同时进行肉眼观察,HE染色和透射电镜等病理学观察,结果表明:试验组脾脏肿胀,线粒体空泡化,淋巴细胞凋亡,网状细胞增生;胸腺萎缩,胸腺小体发育迟缓、肿胀,胸腺小体的细胞内线粒体肿胀,皮质部变薄;法氏囊发育迟缓,体积小,淋巴细胞凋亡和网状细胞增生。
感染后1w、2w、3w、4w、5w采抗凝血1mL,分别用E-玫瑰花环实验和流式细胞仪进行T淋巴细胞计数和CD分子检测,结果显示:试验组玫瑰花环率显著低于对照组;试验组CD3+显著低于对照组,CD4+T前23天高于对照组,以后低于对照组,CD8+T明显高于对照组,CD4+/CD8+明显低于对照组。感染CSISV肉仔鸡一周后接种ND疫苗,免疫后1w、2w、3w、4w采血进行血凝抑制实验,检测抗体,结果试验组鸡ND免疫抗体滴度显著低于对照组。
综上所述CSISV对肉仔鸡体液免疫和细胞免疫都产生抑制作用。
Recently years ,Chicken stunt and immunosupression syndrome is one of important infectious diseases ,its pathogenicity and affect of body is mainstay of prevention and cure it . The research founded on that CSIS is a new disease which is proved by professor Xing-you Liu ,is about to study the pathogenicity to major immune organs and affect to immune function of broiler chickens.
Three-days-old broiler chicken(American ROSS308) is artificial infected by CSISV. On 24、48、72、96、120、144 hours et al. after artificial infection, the index of organ is measured about three major immune organs: spleen、thymus、bursa of fabricius, meanwhile, by macroscopic observation, H.E. dyeing、transmission electron microscope observation. The result shows that spleen of infected group swell, mitochondria vacuolization, lymphocyte apoptosis, reticulocyte proliferation; Atrophy of thymus gland, hypoevolutism、swell of concentric corpuscles, swell of concentric corpuscles cell's mitochondria, attenuation of cortical part; hypoevolutism of bursa of Fabricius,volume growing down ,lymphocyte apoptosis ,reticulocyte proliferation.
One milliliter anticoagulated blood is gotten from broiler chicken on one week, two weeks、three weeks , four weeks, five weeks after artificial infection CSISV. lymphocyte count by rosettes experiment and detection of CD molecule, The consequence is chaplet rate of infected group, is significantly lower than control group;CD3+of infected group is significantly lower than control group, CD4+Tof infected group is higher than that of control group first twenty three days, after that, infected group is lower than control goup.CD8+T of infected group is higher than that of control group,CD4+/CD8+ of infected group is lower than that of control group.
Broiler chickens on one week after artificial infection CSISV is inoculated newcastle disease vaccine. after inoculation, the blood is gotten on one week/two weeks/three weeks/four weeks ,to detect antibody by hemagglution inhibition experiment. The result is that ND antibody titer of infected group is significant lower than that of control group.
To sum up,humoral immunity and cell immunity of artificial infection CSISV chicken are inhibited.
将CSISV人工感染3日龄肉仔鸡(美国ROSS308),分别在感染后24、48、72、96、120、144h等采取脾脏、胸腺和法氏囊,测定其器官指数,同时进行肉眼观察,HE染色和透射电镜等病理学观察,结果表明:试验组脾脏肿胀,线粒体空泡化,淋巴细胞凋亡,网状细胞增生;胸腺萎缩,胸腺小体发育迟缓、肿胀,胸腺小体的细胞内线粒体肿胀,皮质部变薄;法氏囊发育迟缓,体积小,淋巴细胞凋亡和网状细胞增生。
感染后1w、2w、3w、4w、5w采抗凝血1mL,分别用E-玫瑰花环实验和流式细胞仪进行T淋巴细胞计数和CD分子检测,结果显示:试验组玫瑰花环率显著低于对照组;试验组CD3+显著低于对照组,CD4+T前23天高于对照组,以后低于对照组,CD8+T明显高于对照组,CD4+/CD8+明显低于对照组。感染CSISV肉仔鸡一周后接种ND疫苗,免疫后1w、2w、3w、4w采血进行血凝抑制实验,检测抗体,结果试验组鸡ND免疫抗体滴度显著低于对照组。
综上所述CSISV对肉仔鸡体液免疫和细胞免疫都产生抑制作用。
Recently years ,Chicken stunt and immunosupression syndrome is one of important infectious diseases ,its pathogenicity and affect of body is mainstay of prevention and cure it . The research founded on that CSIS is a new disease which is proved by professor Xing-you Liu ,is about to study the pathogenicity to major immune organs and affect to immune function of broiler chickens.
Three-days-old broiler chicken(American ROSS308) is artificial infected by CSISV. On 24、48、72、96、120、144 hours et al. after artificial infection, the index of organ is measured about three major immune organs: spleen、thymus、bursa of fabricius, meanwhile, by macroscopic observation, H.E. dyeing、transmission electron microscope observation. The result shows that spleen of infected group swell, mitochondria vacuolization, lymphocyte apoptosis, reticulocyte proliferation; Atrophy of thymus gland, hypoevolutism、swell of concentric corpuscles, swell of concentric corpuscles cell's mitochondria, attenuation of cortical part; hypoevolutism of bursa of Fabricius,volume growing down ,lymphocyte apoptosis ,reticulocyte proliferation.
One milliliter anticoagulated blood is gotten from broiler chicken on one week, two weeks、three weeks , four weeks, five weeks after artificial infection CSISV. lymphocyte count by rosettes experiment and detection of CD molecule, The consequence is chaplet rate of infected group, is significantly lower than control group;CD3+of infected group is significantly lower than control group, CD4+Tof infected group is higher than that of control group first twenty three days, after that, infected group is lower than control goup.CD8+T of infected group is higher than that of control group,CD4+/CD8+ of infected group is lower than that of control group.
Broiler chickens on one week after artificial infection CSISV is inoculated newcastle disease vaccine. after inoculation, the blood is gotten on one week/two weeks/three weeks/four weeks ,to detect antibody by hemagglution inhibition experiment. The result is that ND antibody titer of infected group is significant lower than that of control group.
To sum up,humoral immunity and cell immunity of artificial infection CSISV chicken are inhibited.
引文
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