皖南花猪和皖南黑猪PRLR和BF基因多态性与产仔性状的关联分析
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摘要
本论文以PRLR(催乳素受体)基因和BF(备解素因子)基因为研究对象,旨在探索这两个基因的多态性与安徽两个地方猪种(皖南花猪、皖南黑猪)高繁殖力之间的关系。
用于本研究的猪分别来自于黄山市黟县皖南花猪保种场和宁国市皖南黑猪保种场,其中皖南花猪30只,皖南黑猪85只。利用限制性内切酶酶切技术研究这些基因在遗传变异及其对猪繁殖性能的影响。结果表明:
(1)对于PRLR基因,根据猪的PRLR基因设计1对引物覆盖该基因外显子10,检测PRLR基因在皖南花猪和皖南黑猪中的单核苷酸多态性,同时研究该基因对猪繁殖力的影响。检测结果显示,在皖南花猪和皖南黑猪中均检测到AA、AB和BB型;皖南花猪AA型、AB型、BB型的基因型频率分别为0.6667、0.2333和0.100;皖南黑猪AA型、AB型、BB型的基因型频率分别为0.5647、0.2824和0.1529。统计分析结果显示不论是皖南花猪还是皖南黑猪BB型与AA型的产仔数差异均显著(P<0.05)。
(2)对于BF基因,针对该基因的内含子1设计了1对引物中,仅引物P8扩增片段存在多态性。皖南花猪共发现两种基因型:CC和CD型,基因型频率为0.5333和0.4667。皖南黑猪共发现三种基因型:CC、CD和DD型。基因频率为0.3295、0.5529和0.1176。BF基因在两个安徽地方猪种的群体遗传分析表明:C为优势基因,在两个种群中占有优势。统计分析结果显示不论是皖南花猪还是皖南黑猪CC型与DD型的产仔数差异均显著(P<0.05)。研究结果初步表明PRLR基因和BF基因皆是提高猪的一个潜在有效的DNA标记。
The PRLR gene and steroid BF gene were studied as candidate genes for the prolificacy in two local breeds of Anhui (Wannanhua Pigs and Wannanhei Pigs) in the present study by techniques of PCR and restriction endonuclease. Samples used in this research represented for high prolificacy breeds (Wannanhua Pigs—30 in total;Wannanhei Pigs—85 in total) were obtained from Conservation Farm for Wannanhua Pigs in Huangshanduo County and Conservation Farm for Wannanhei Pigs in Ningguo respectively.
(1)As for PRLR gene, one pair of primer was designed to detect single nucleotide polymorphisms of exon 10 of PRLR gene in Wannanhua Pigs and Wannanhchangei Pigs by PCR and digestion with restriction endonucleases according to the sequence of swine PRLR gene. Three genotypes (AA, AB and BB) were detected in both Wannanhua Pigs and Wannanhei Pigs. The gene frequency of AA,AB and BB were 0.667,0.233 and 0.100 in Wannanhua Pigs while in Wannanhei Pigs were 0.5647, 0.2824 and 0.1529. According to statistic analysis, the differences of the litter size in Wannanhua Pigs and Wannanhei Pigs between BB and AA genotypes were all significant (P<0.05).
(2)As for BF gene, one pair of primer was designed to detect single nucleotide polymorphisms of intron 1 of BF gene in Wannanhua Pigs and Wannanhei Pigs by PCR and digestion with restriction endonucleases according to the sequence of swine BF gene. Two genotypes (CC and DD) were detected in Wannanhua Pigs (gene frequency were 0.5333 and0.4667 respectively) while Three genotypes (CC, DD and CD) were detected in Wannanhei Pigs(gene frequency were 0.3295,0.5529 and 0.1176 respectively). According to statistic analysis, allele C had a positive heterosis in these two breeds and the differences of the litter size in Wannanhua Pigs and Wannanhei Pigs between CC and DD genotypes, CC and CD genotypes were all significant (P<0.05). These results preliminarily indicated that the PRLR gene and BF gene are potential DNA markers for improving litter size in pigs.
用于本研究的猪分别来自于黄山市黟县皖南花猪保种场和宁国市皖南黑猪保种场,其中皖南花猪30只,皖南黑猪85只。利用限制性内切酶酶切技术研究这些基因在遗传变异及其对猪繁殖性能的影响。结果表明:
(1)对于PRLR基因,根据猪的PRLR基因设计1对引物覆盖该基因外显子10,检测PRLR基因在皖南花猪和皖南黑猪中的单核苷酸多态性,同时研究该基因对猪繁殖力的影响。检测结果显示,在皖南花猪和皖南黑猪中均检测到AA、AB和BB型;皖南花猪AA型、AB型、BB型的基因型频率分别为0.6667、0.2333和0.100;皖南黑猪AA型、AB型、BB型的基因型频率分别为0.5647、0.2824和0.1529。统计分析结果显示不论是皖南花猪还是皖南黑猪BB型与AA型的产仔数差异均显著(P<0.05)。
(2)对于BF基因,针对该基因的内含子1设计了1对引物中,仅引物P8扩增片段存在多态性。皖南花猪共发现两种基因型:CC和CD型,基因型频率为0.5333和0.4667。皖南黑猪共发现三种基因型:CC、CD和DD型。基因频率为0.3295、0.5529和0.1176。BF基因在两个安徽地方猪种的群体遗传分析表明:C为优势基因,在两个种群中占有优势。统计分析结果显示不论是皖南花猪还是皖南黑猪CC型与DD型的产仔数差异均显著(P<0.05)。研究结果初步表明PRLR基因和BF基因皆是提高猪的一个潜在有效的DNA标记。
The PRLR gene and steroid BF gene were studied as candidate genes for the prolificacy in two local breeds of Anhui (Wannanhua Pigs and Wannanhei Pigs) in the present study by techniques of PCR and restriction endonuclease. Samples used in this research represented for high prolificacy breeds (Wannanhua Pigs—30 in total;Wannanhei Pigs—85 in total) were obtained from Conservation Farm for Wannanhua Pigs in Huangshanduo County and Conservation Farm for Wannanhei Pigs in Ningguo respectively.
(1)As for PRLR gene, one pair of primer was designed to detect single nucleotide polymorphisms of exon 10 of PRLR gene in Wannanhua Pigs and Wannanhchangei Pigs by PCR and digestion with restriction endonucleases according to the sequence of swine PRLR gene. Three genotypes (AA, AB and BB) were detected in both Wannanhua Pigs and Wannanhei Pigs. The gene frequency of AA,AB and BB were 0.667,0.233 and 0.100 in Wannanhua Pigs while in Wannanhei Pigs were 0.5647, 0.2824 and 0.1529. According to statistic analysis, the differences of the litter size in Wannanhua Pigs and Wannanhei Pigs between BB and AA genotypes were all significant (P<0.05).
(2)As for BF gene, one pair of primer was designed to detect single nucleotide polymorphisms of intron 1 of BF gene in Wannanhua Pigs and Wannanhei Pigs by PCR and digestion with restriction endonucleases according to the sequence of swine BF gene. Two genotypes (CC and DD) were detected in Wannanhua Pigs (gene frequency were 0.5333 and0.4667 respectively) while Three genotypes (CC, DD and CD) were detected in Wannanhei Pigs(gene frequency were 0.3295,0.5529 and 0.1176 respectively). According to statistic analysis, allele C had a positive heterosis in these two breeds and the differences of the litter size in Wannanhua Pigs and Wannanhei Pigs between CC and DD genotypes, CC and CD genotypes were all significant (P<0.05). These results preliminarily indicated that the PRLR gene and BF gene are potential DNA markers for improving litter size in pigs.
引文
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[13] Blowe CD.Boytte KE.Ashwell MS.et al.Characterization of a line of pigs previously selected for increased litter size for RBP4 and follistatin[J]. Anim Breed Genet,2006,123(6):389-395
[14] Pinton P,Schibler L,Cribiu E, et al.Localuzatuib if 113 anchor loci in pigs:improvement of the comparative map for humans[J].pig.and goats.Mamm Genome,2000,11(4):306-315
[15] Vincent AL,Wng L,Tuggle C K,et al.Prolactin receptor maps to pig chromosome16[J].Mammalian Genome.1997,8:793-794
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[17] Berschinger H.U.Bachmann,M,Mettle.C,et al.Adhesive fimbriae produced in vivo by Escherichiacoli O139:K12(B):H1 associated with eneterotoxaemia in pigs.Vet Microbiology.1990,25:267-281.
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