荧光有机小分子试剂在磷酸根阴离子家族典型成员分析中的应用研究
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摘要
荧光有机小分子化合物由于具有很好的光学特性,在阴离子的分析中得到广泛的应用。筛选或合成新的特异性识别检测阴离子的荧光有机小分子是近年来超分子化学领域的发展方向和趋势。本文以含磷酸根阴离子为研究对象,探讨了藏红T分子与六偏磷酸根的相互作用,并根据藏红T作用前后光谱的变化建立了两种简单、快速检测六偏磷酸钠的新方法。此外,合成了缩邻氨基苯酚化合物,研究了它与焦磷酸盐的作用。具体包括以下三个方面：
(1)研究了藏红T与六偏磷酸钠分别在弱酸性的NaAc-HAc缓冲及弱碱性的Tris-HCl缓冲中的相互作用,结果发现,在两种介质中随着六偏磷酸钠的加入,藏红T在520nm的吸收峰强度均明显降低,同时在476nm处出现新的吸收峰；实验还发现加入六偏磷酸钠后,藏红T的在578nm处荧光强度会降低,但是其荧光寿命没有发生变化,表明SHMP静态猝灭ST的荧光。据此,在弱酸性条件下根据SHMP存在时ST吸光度的变化,建立了一种检测食品添加剂六偏磷酸钠的新方法,线性范围为1.0×10-6mol/L-1.1×10-5mol/L,检测限为3.4×10-7mol/L。该方法应用于饮料中六偏磷酸钠的检测,相对误差小于4.6%,回收率在95.0%-104.0%之间。
(2)在pH8.5的Tris-HCl缓冲溶液,藏红T(ST)与钙黄绿素(Calcein)能发生有效地荧光共振能量转移,而六偏磷酸钠(SHMP)的加入使得藏红T荧光猝灭,同时增大了ST与Calcein之间的距离,导致了荧光共振能量转移效率的降低。六偏磷酸钠的浓度在3.0×10-6mol/L-1.0×10-5mol/L范围内呈很好的线性关系,对6.0×10-6mol/L的连续平行测定11次,其相对标准偏差(RSD)为3.1%,应用于饮料中六偏磷酸钠的分析检测,结果令人满意。
(3)合成荧光试剂缩水杨醛邻氨基苯酚,在pH 5.0的NaAc-HAc缓冲溶液中,考察了该试剂与Br-、Cl-、ClO3-、HCO3-、NO3-、Pi、SO2-、CTP、GTP、UTP、H2PO4-、ppi等阴离子的相互作用,结果表明该试剂只对ppi有较好的选择性识别能力,ppi的加入导致缩水杨醛邻氨基苯酚溶液荧光强度降低,据此建立了一种检测ppi的新方法。该方法可用于合成样中ppi的检测,回收率在95%以上。
Because of the good optical properties, the fluorescent and small organic molecules have been widely applied in the fields of anionic analysis. Specific screening or synthesis of new fluorescent and small organic molecules for anionic recognition and detection supply much more opportunities and challenges in this evolution for supramolecular chemistry in recent years. In this thesis, the typical members in phosphate anion family is the main object of the study. We have discussed the interaction between ST and SHMP, in accordance with changes in spectra of ST, two newly, rapidly and simplely methods was established for SHMP's detection. In addition, fluorescence reagent Salicylaldehyde-o-Aminophenol was synthesized. With the addition of ppi, the fluorescent intensity of Salicylaldehyde-o-Aminophenol was decreased. A direct fluorescence analytical method was proposed to determine the ppi based on the decrease of fluorescent intensity with the increase of the concentration of ppi. The mainly points are as follows:
(1) We have respectively researched the interaction between ST and SHMP in the acid HAc-NaAc buffer and alkaline Tris-HCl buffer. The results showed that the absorbency of ST was decreased at 520nm after adding the SHMP, And a new peak appeared at 476 nm in visible absorption spectra. Further found that the fluorescent intensity of ST was decreased at 578nm, but the fluorescent lifetime of the ST is not changed, which showed the fluorescence of ST is statically quenched by SHMP. Based on the change of absorbance, we have established a new method to detect the SHMP. Under the optimized conditions, the linear range was 1.0×10-6 mol/L-1.1×10-5 mol/L with the detection limit of 3.4×10-7mol/L. The application of the established method in the determination of tea drink samples gave a recovery rate of 95.0%-104.0%, with a relative standard deviation of less than 4.6%.
(2) In a medium of pH 8.5 tris-HCl buffer, it was found that safranine T (ST) can react with calcein to efficiently produce fluorescence resonance energy transfer (FRET), safranine T get aggregation and its fluorescence receded after adding sodium hexametaphosphat(SHMP), which results in a decrease of the FRET between safranine T and calcein. The ratio of fluorescent intensity at 520nm and 578nm was linear with the concentration of melamine over the range of 3.0×10-6-1.0×10-5 mol/L. the Relative Standard Deviation (RSD) was 3.1% for determination of 6.0×10-6mol/L sodium hexametaphosphat (n=11). The proposed method is high selectively, simply and rapid. it was successfully applied to determine the concentration of two synthetic samples.
(3) Fluorescence reagent salicylaldehyde-o-aminophenol was synthesized. In the buffer solution medium of HAc-NaAc at pH 5.0, the reagent can react with ppi more selective than other anions, such as Br-、Cl-、ClO3-、HCO3-、NO3-Pi、SO2-、CTP、GTP、UTP、H2PO4-、ppi. The complex compound made fluorescence intensity decrease and the degree of decreasing was linear with the concentration of ppi. Based on this, a new method for the direct determination of ppi was established. This method could be used to detect synthetic samples, and the recovery was larger than 95%.
(1)研究了藏红T与六偏磷酸钠分别在弱酸性的NaAc-HAc缓冲及弱碱性的Tris-HCl缓冲中的相互作用,结果发现,在两种介质中随着六偏磷酸钠的加入,藏红T在520nm的吸收峰强度均明显降低,同时在476nm处出现新的吸收峰；实验还发现加入六偏磷酸钠后,藏红T的在578nm处荧光强度会降低,但是其荧光寿命没有发生变化,表明SHMP静态猝灭ST的荧光。据此,在弱酸性条件下根据SHMP存在时ST吸光度的变化,建立了一种检测食品添加剂六偏磷酸钠的新方法,线性范围为1.0×10-6mol/L-1.1×10-5mol/L,检测限为3.4×10-7mol/L。该方法应用于饮料中六偏磷酸钠的检测,相对误差小于4.6%,回收率在95.0%-104.0%之间。
(2)在pH8.5的Tris-HCl缓冲溶液,藏红T(ST)与钙黄绿素(Calcein)能发生有效地荧光共振能量转移,而六偏磷酸钠(SHMP)的加入使得藏红T荧光猝灭,同时增大了ST与Calcein之间的距离,导致了荧光共振能量转移效率的降低。六偏磷酸钠的浓度在3.0×10-6mol/L-1.0×10-5mol/L范围内呈很好的线性关系,对6.0×10-6mol/L的连续平行测定11次,其相对标准偏差(RSD)为3.1%,应用于饮料中六偏磷酸钠的分析检测,结果令人满意。
(3)合成荧光试剂缩水杨醛邻氨基苯酚,在pH 5.0的NaAc-HAc缓冲溶液中,考察了该试剂与Br-、Cl-、ClO3-、HCO3-、NO3-、Pi、SO2-、CTP、GTP、UTP、H2PO4-、ppi等阴离子的相互作用,结果表明该试剂只对ppi有较好的选择性识别能力,ppi的加入导致缩水杨醛邻氨基苯酚溶液荧光强度降低,据此建立了一种检测ppi的新方法。该方法可用于合成样中ppi的检测,回收率在95%以上。
Because of the good optical properties, the fluorescent and small organic molecules have been widely applied in the fields of anionic analysis. Specific screening or synthesis of new fluorescent and small organic molecules for anionic recognition and detection supply much more opportunities and challenges in this evolution for supramolecular chemistry in recent years. In this thesis, the typical members in phosphate anion family is the main object of the study. We have discussed the interaction between ST and SHMP, in accordance with changes in spectra of ST, two newly, rapidly and simplely methods was established for SHMP's detection. In addition, fluorescence reagent Salicylaldehyde-o-Aminophenol was synthesized. With the addition of ppi, the fluorescent intensity of Salicylaldehyde-o-Aminophenol was decreased. A direct fluorescence analytical method was proposed to determine the ppi based on the decrease of fluorescent intensity with the increase of the concentration of ppi. The mainly points are as follows:
(1) We have respectively researched the interaction between ST and SHMP in the acid HAc-NaAc buffer and alkaline Tris-HCl buffer. The results showed that the absorbency of ST was decreased at 520nm after adding the SHMP, And a new peak appeared at 476 nm in visible absorption spectra. Further found that the fluorescent intensity of ST was decreased at 578nm, but the fluorescent lifetime of the ST is not changed, which showed the fluorescence of ST is statically quenched by SHMP. Based on the change of absorbance, we have established a new method to detect the SHMP. Under the optimized conditions, the linear range was 1.0×10-6 mol/L-1.1×10-5 mol/L with the detection limit of 3.4×10-7mol/L. The application of the established method in the determination of tea drink samples gave a recovery rate of 95.0%-104.0%, with a relative standard deviation of less than 4.6%.
(2) In a medium of pH 8.5 tris-HCl buffer, it was found that safranine T (ST) can react with calcein to efficiently produce fluorescence resonance energy transfer (FRET), safranine T get aggregation and its fluorescence receded after adding sodium hexametaphosphat(SHMP), which results in a decrease of the FRET between safranine T and calcein. The ratio of fluorescent intensity at 520nm and 578nm was linear with the concentration of melamine over the range of 3.0×10-6-1.0×10-5 mol/L. the Relative Standard Deviation (RSD) was 3.1% for determination of 6.0×10-6mol/L sodium hexametaphosphat (n=11). The proposed method is high selectively, simply and rapid. it was successfully applied to determine the concentration of two synthetic samples.
(3) Fluorescence reagent salicylaldehyde-o-aminophenol was synthesized. In the buffer solution medium of HAc-NaAc at pH 5.0, the reagent can react with ppi more selective than other anions, such as Br-、Cl-、ClO3-、HCO3-、NO3-Pi、SO2-、CTP、GTP、UTP、H2PO4-、ppi. The complex compound made fluorescence intensity decrease and the degree of decreasing was linear with the concentration of ppi. Based on this, a new method for the direct determination of ppi was established. This method could be used to detect synthetic samples, and the recovery was larger than 95%.
引文
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