补肾法对肾虚排卵抑制模型大鼠的影响
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摘要
目的:
以SD大鼠肾虚排卵抑制模型为研究工具,以罗氏促排卵汤和/或针灸补肾法为施加因素,研究补肾法对肾虚排卵抑制模型的促排卵作用,探讨补肾法治疗排卵障碍不孕症的机理。
方法:
采用羟基脲灌胃方法对10-12周龄,体重200-250g,SPF级雌性SD大鼠进行造模,造模后观察阴道细胞涂片与卵巢的病理改变,在光镜下计算卵巢切片各级卵泡计数,造模29天大鼠卵巢组织中次级生长卵泡与成熟卵泡的比例明显减少,而以原始卵泡和初级生长卵泡为主。提示为排卵抑制,造模成功。
将造模成功大鼠随机分为4组,分为①肾虚排卵障碍造模组、②造模加中药组、③造模加针刺组、④造模加针刺加中药组,再加上未造模的空白对照组,共5组。药物和(或)针灸干预的第6天,各组分别取一半雌鼠与雄鼠同笼;药物和(或)针灸干预的第15天,未与雄鼠同笼的大鼠,无菌条件下处死,采血,进行血清性激素、血清抑制素A、B的检测;取各组大鼠脏器测湿重;药物和(或)针灸干预的第15天,与雄鼠同笼者,取出雄鼠,继续饲养7天后无菌条件下处死,采血和测定血清性激素、脏器测湿重。
结果:
1、羟基脲造模后可使大鼠出现肾虚症状,活动减少,体重增加缓慢,模型组大鼠体重低于正常对照组。
2、羟基脲造模成功后,予以罗氏促排卵汤煎剂、针刺关元、中极、肾俞、子宫穴位加电针、罗氏促排卵汤煎剂+针刺加电针进行治疗干预,均可以使大鼠子宫、卵巢湿重有不同程度的增加,而肾、肾上腺、脾、脑垂体的变化不明显;治疗干预后与雄鼠同笼大鼠妊娠率提高,妊娠胚胎数,单个子胎直径有不同程度的增加。
3.各组大鼠血清性激素、抑制素A、B的变化,组间比较均未见明显统计学意义(P＞0.05)。
结论:
通过对SD大鼠羟基脲造模形成肾虚排卵障碍的动物模型进行补肾治疗的实验研究,可以得出以下结论:
1.应用羟基脲灌胃可以成功建立肾虚排卵抑制大鼠动物模型,并以肾虚症状为主,兼见脾虚的症状。
2.通过补肾法对肾虚-排卵抑制病理模型进行治疗,可以有效改善模型大鼠的肾虚及脾虚症状。
3.本研究采用的补肾法包括以附子、菟丝子、巴戟、淫羊藿等为主要药物的中药复方罗氏促排卵汤;针刺取关元、中极、肾俞、子宫等穴;也采用了针、药并用的治法,对肾虚-排卵抑制病理模型进行治疗干预,可以有效提高大鼠的妊娠率、妊娠胚胎数目、单个胚胎直径,但对大鼠各个脏器湿重、内分泌和抑制素等指标的影响尚未有统计学意义。
4.各组大鼠血清性激素、抑制素A、B的变化,组间比较均未见明显统计学意义,说明在大鼠的育龄早期应用羟基脲灌胃建立肾虚排卵抑制模型,并未影响到模型大鼠的卵巢储备能力。
Objective:
The research focused on invigorating kidney therapy applying on rat model with renal deficiency and ovulation inhibition built by Hydroxyurea for stimulating ovulation.The therapies for invigorating kidney are including herbal medical formula, acupuncture,both herbal formula and acupuncture.This study would make sense in mechanism of Chinese medicine for female infertility treatment on ovulation inhibition.
Method.
SPF SD female rats,10-12weeks old,weight 220-250g,were made by Hydroxyurea for stimulating ovulation.After intragastric administration of hydroxycarbarnide,Vaginal cells in smear and pathological changes of ovary were observed.Successful model has prompted by inhibiting ovulation pathology,which the proportion of secondary growing follicles and mature follicles was reduced in evidence,primordial follicles and primary growing follicles were in the majority in ovary when the numbers of follicles of all levels were counted through light microscope in the 29~(th) day.
Successful model rats were distributed into 4 groups at random,which were group A:model,B:model +medicine,C.model +acupuncture,D.model + medicine + acupuncture,adding E:control,which were not intragastric administration. There were 5 groups all.Rat model with renal deficiency were given invigorating kidney therapies,including herbal medical formula,acupuncture,both herbal formula and acupuncture.Herbal medical formula and(or) acupuncture intervention in the first 6 days,half of each group rat were taken female and male with cage;In the first 15 days,rat which were not with the male rat with cage were killed in asepsis condition, and the following date was measured:blood-serum sex hormones and inhibin,wet weight of uterine,ovarian,kidney,spleen,adrenal and pituitary.At the same day,rat which were with the male rat with cage were taken out of the cage.After 7days,rat which were with the male rat with cage were killed in asepsis condition,the following date was measured:blood-serum sex hormones,wet weight of organs,pregnancy rate.
Results,
1.Model rat with intragastric administration of hydroxycarbamide expressed symptoms of renal deficiency,less activity,slower weight increase,weight of model rats were lower compared with control group.
2.Invigorating kidney therapies were taken on successful model rat with renal deficiency and ovulation inhibition,involving promoting ovulation herbal medical formula of Luo,acupuncture of Guanyuan,Zhongji,Shenshu,Zigong points,both herbal formula and acupuncture.Wet weights of uterine and ovarian were increased in varying degrees compared with model group,but wet weights of kidney,spleen, adrenal,pituitary did not increase in significant difference.The pregnancy rates,the number of pregnancy embryos and the diameter of a single embryo were also increased.
3.There were no significant difference in blood-serum sex hormones and inhibin A and B in all groups(P＞0.05).
Conclusion:
Conclusions were educed on research of invigorating kidney therapy applying on rat model with renal deficiency and ovulation inhibition built by Hydroxyurea for stimulating ovulation.
1.Model rat with intragastric administration of hydroxycarbamide were successfully built,symptoms of deficiency both renal and spleen were showed.
2.Nourish kidney therapies effectively improved the symptoms of deficiency both renal and spleen after treatment.
3.Nourish kidney therapies involved promoting ovulation herbal compound medical formula of Luo which has radix aconiti carmichaeli,semen cuscutae,radices morindae,herba epimedii,and so on,acupuncture of Guanyuan,Zhongji,Shenshu, Zigong points and both herbal formula and acupuncture.Pregnancy rate,number of pregnancy embryos,diameter of a single embryo of treatment groups improved effectively.But there were no significant difference in organ wet weights, blood-serum sex hormones and inhibin in all groups.
4.There were no significant difference in organ wet weights,blood-serum sex hormones and inhibin in all groups,which could illustrate model rat with intragastric administration of hydroxycarbamide,which were at the early reproductive life,did not influence the reserve capacity of ovarian..
以SD大鼠肾虚排卵抑制模型为研究工具,以罗氏促排卵汤和/或针灸补肾法为施加因素,研究补肾法对肾虚排卵抑制模型的促排卵作用,探讨补肾法治疗排卵障碍不孕症的机理。
方法:
采用羟基脲灌胃方法对10-12周龄,体重200-250g,SPF级雌性SD大鼠进行造模,造模后观察阴道细胞涂片与卵巢的病理改变,在光镜下计算卵巢切片各级卵泡计数,造模29天大鼠卵巢组织中次级生长卵泡与成熟卵泡的比例明显减少,而以原始卵泡和初级生长卵泡为主。提示为排卵抑制,造模成功。
将造模成功大鼠随机分为4组,分为①肾虚排卵障碍造模组、②造模加中药组、③造模加针刺组、④造模加针刺加中药组,再加上未造模的空白对照组,共5组。药物和(或)针灸干预的第6天,各组分别取一半雌鼠与雄鼠同笼;药物和(或)针灸干预的第15天,未与雄鼠同笼的大鼠,无菌条件下处死,采血,进行血清性激素、血清抑制素A、B的检测;取各组大鼠脏器测湿重;药物和(或)针灸干预的第15天,与雄鼠同笼者,取出雄鼠,继续饲养7天后无菌条件下处死,采血和测定血清性激素、脏器测湿重。
结果:
1、羟基脲造模后可使大鼠出现肾虚症状,活动减少,体重增加缓慢,模型组大鼠体重低于正常对照组。
2、羟基脲造模成功后,予以罗氏促排卵汤煎剂、针刺关元、中极、肾俞、子宫穴位加电针、罗氏促排卵汤煎剂+针刺加电针进行治疗干预,均可以使大鼠子宫、卵巢湿重有不同程度的增加,而肾、肾上腺、脾、脑垂体的变化不明显;治疗干预后与雄鼠同笼大鼠妊娠率提高,妊娠胚胎数,单个子胎直径有不同程度的增加。
3.各组大鼠血清性激素、抑制素A、B的变化,组间比较均未见明显统计学意义(P＞0.05)。
结论:
通过对SD大鼠羟基脲造模形成肾虚排卵障碍的动物模型进行补肾治疗的实验研究,可以得出以下结论:
1.应用羟基脲灌胃可以成功建立肾虚排卵抑制大鼠动物模型,并以肾虚症状为主,兼见脾虚的症状。
2.通过补肾法对肾虚-排卵抑制病理模型进行治疗,可以有效改善模型大鼠的肾虚及脾虚症状。
3.本研究采用的补肾法包括以附子、菟丝子、巴戟、淫羊藿等为主要药物的中药复方罗氏促排卵汤;针刺取关元、中极、肾俞、子宫等穴;也采用了针、药并用的治法,对肾虚-排卵抑制病理模型进行治疗干预,可以有效提高大鼠的妊娠率、妊娠胚胎数目、单个胚胎直径,但对大鼠各个脏器湿重、内分泌和抑制素等指标的影响尚未有统计学意义。
4.各组大鼠血清性激素、抑制素A、B的变化,组间比较均未见明显统计学意义,说明在大鼠的育龄早期应用羟基脲灌胃建立肾虚排卵抑制模型,并未影响到模型大鼠的卵巢储备能力。
Objective:
The research focused on invigorating kidney therapy applying on rat model with renal deficiency and ovulation inhibition built by Hydroxyurea for stimulating ovulation.The therapies for invigorating kidney are including herbal medical formula, acupuncture,both herbal formula and acupuncture.This study would make sense in mechanism of Chinese medicine for female infertility treatment on ovulation inhibition.
Method.
SPF SD female rats,10-12weeks old,weight 220-250g,were made by Hydroxyurea for stimulating ovulation.After intragastric administration of hydroxycarbarnide,Vaginal cells in smear and pathological changes of ovary were observed.Successful model has prompted by inhibiting ovulation pathology,which the proportion of secondary growing follicles and mature follicles was reduced in evidence,primordial follicles and primary growing follicles were in the majority in ovary when the numbers of follicles of all levels were counted through light microscope in the 29~(th) day.
Successful model rats were distributed into 4 groups at random,which were group A:model,B:model +medicine,C.model +acupuncture,D.model + medicine + acupuncture,adding E:control,which were not intragastric administration. There were 5 groups all.Rat model with renal deficiency were given invigorating kidney therapies,including herbal medical formula,acupuncture,both herbal formula and acupuncture.Herbal medical formula and(or) acupuncture intervention in the first 6 days,half of each group rat were taken female and male with cage;In the first 15 days,rat which were not with the male rat with cage were killed in asepsis condition, and the following date was measured:blood-serum sex hormones and inhibin,wet weight of uterine,ovarian,kidney,spleen,adrenal and pituitary.At the same day,rat which were with the male rat with cage were taken out of the cage.After 7days,rat which were with the male rat with cage were killed in asepsis condition,the following date was measured:blood-serum sex hormones,wet weight of organs,pregnancy rate.
Results,
1.Model rat with intragastric administration of hydroxycarbamide expressed symptoms of renal deficiency,less activity,slower weight increase,weight of model rats were lower compared with control group.
2.Invigorating kidney therapies were taken on successful model rat with renal deficiency and ovulation inhibition,involving promoting ovulation herbal medical formula of Luo,acupuncture of Guanyuan,Zhongji,Shenshu,Zigong points,both herbal formula and acupuncture.Wet weights of uterine and ovarian were increased in varying degrees compared with model group,but wet weights of kidney,spleen, adrenal,pituitary did not increase in significant difference.The pregnancy rates,the number of pregnancy embryos and the diameter of a single embryo were also increased.
3.There were no significant difference in blood-serum sex hormones and inhibin A and B in all groups(P＞0.05).
Conclusion:
Conclusions were educed on research of invigorating kidney therapy applying on rat model with renal deficiency and ovulation inhibition built by Hydroxyurea for stimulating ovulation.
1.Model rat with intragastric administration of hydroxycarbamide were successfully built,symptoms of deficiency both renal and spleen were showed.
2.Nourish kidney therapies effectively improved the symptoms of deficiency both renal and spleen after treatment.
3.Nourish kidney therapies involved promoting ovulation herbal compound medical formula of Luo which has radix aconiti carmichaeli,semen cuscutae,radices morindae,herba epimedii,and so on,acupuncture of Guanyuan,Zhongji,Shenshu, Zigong points and both herbal formula and acupuncture.Pregnancy rate,number of pregnancy embryos,diameter of a single embryo of treatment groups improved effectively.But there were no significant difference in organ wet weights, blood-serum sex hormones and inhibin in all groups.
4.There were no significant difference in organ wet weights,blood-serum sex hormones and inhibin in all groups,which could illustrate model rat with intragastric administration of hydroxycarbamide,which were at the early reproductive life,did not influence the reserve capacity of ovarian..
引文
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