内蒙地区蒙古族儿童过敏性紫癜与HLA-A基因的关联性
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摘要
目的探索内蒙地区蒙古族儿童过敏性紫癜(AP)与HLA-A等位基因的关联性,可望找出相关基因,以探寻AP的部分发病机理及其防治前景。方法采用病例对照研究策略,引入PCR-SSO技术,在祖籍三代居住内蒙地区,无血缘关系,无与异族通婚史及其他免疫性疾病史和家族史的蒙古族人群中,选择儿童AP病例组56例和健康儿童对照组66名,作以HLA-A等位基因的型别分析。基因频率比较在单因素四格表x2检验或Fisher检验的基础上,再作以Logistic多因素回归分析。结果病例组HLA-A*11等位基因频率为16.1%,与对照组的9.1%比较,Wald为3.954,P为0.047<0.05,差异有统计学意义。表明该基因频率在病例组高于对照组,B为0.844>0,OR是2.325>1,促进发病,其95%可信区间为1.012～5.340,其内不包含1,与P意义相符,有统计学意义。EF为0.342>0,结论HLA-A*11等位基因可能是内蒙地区蒙古族儿童AP发病单体型中的一个遗传易感基因。
Objective To explore the association between HLA-A allele and Anaphylactoid Pur-pura(AP)in children of Mongolia in Inner Mongolia. We want to find correlated genes and study part of pathogenesis and the method of prevention and cure of AP.Method We a-dopted the method of case control and we selected 56 children AP as case group and health children 66 as control group in Mongolia,who had resided in Inner Mongolia three generations without consanguinity,history of mixed,marriages,other medical history,and family history of immunity,led into polymerase chain reaction sequence specific oligonucleotide probes(PCR-SSO)technique,analyzed the type of HLA-A allele.The compare of gene frenquency made with logistic regression after x2 or Fisher test. Result The gene frenquency of HLA-A*11 allele in case group(16.1%)compared to that of control group(9.1%),Wald of HLA-A* 11 gene was 3.954,P=0.047,the difference had statistical significance.B=0.844>0,OR =2.325>1,it helped development of the disease.whose 95% confident interval was 1-012-5.340,and which did not include 1,it was statistical significance,EF=0.342>0.Conclu-sion HLA-A*11 allele may be the haplotype susceptible gene of AP in children of Mon-golia in Inner Mongolia.
Objective To explore the association between HLA-A allele and Anaphylactoid Pur-pura(AP)in children of Mongolia in Inner Mongolia. We want to find correlated genes and study part of pathogenesis and the method of prevention and cure of AP.Method We a-dopted the method of case control and we selected 56 children AP as case group and health children 66 as control group in Mongolia,who had resided in Inner Mongolia three generations without consanguinity,history of mixed,marriages,other medical history,and family history of immunity,led into polymerase chain reaction sequence specific oligonucleotide probes(PCR-SSO)technique,analyzed the type of HLA-A allele.The compare of gene frenquency made with logistic regression after x2 or Fisher test. Result The gene frenquency of HLA-A*11 allele in case group(16.1%)compared to that of control group(9.1%),Wald of HLA-A* 11 gene was 3.954,P=0.047,the difference had statistical significance.B=0.844>0,OR =2.325>1,it helped development of the disease.whose 95% confident interval was 1-012-5.340,and which did not include 1,it was statistical significance,EF=0.342>0.Conclu-sion HLA-A*11 allele may be the haplotype susceptible gene of AP in children of Mon-golia in Inner Mongolia.
引文
[1]Halling SF,Soderbery MP,Berg UB.Henoch-Schoenlein nephritis:Clinical finding related to renal function and morphology. Pediatr Nephrol.2005,20(1);46-51.
[2]Beck S,Geraghty D,Inoko H,et al. Complete sequence and gene map of a human major histocompatibility complex[J].Nature,1999,401(28):921.
[3]任少敏,杨光路,仝林虎等.蒙汉族儿童AP临床特点与HLA-DRB1基因关联性对比分析[J].中华风湿病学杂志,2003,7(8)：469.
[4]Amoli MM,Thomson W,Hajeer AH,et al. HLA-B35 association with nephritis in Henoch-Schoenlein purpura[J].J Rheumatol.2002,29(5):948-949.
[5]胡亚美,江载芳,诸福棠实用儿科学.上册[M].第7版.北京：人民出版社,2002,688-690.
[6]谭建明,周永昌,唐孝达,主编.组织配型技术与临床应用[M].北京：人民卫生出版社,2002,317.
[7]王晓娟,林娜,孟化,等.HLA-DQA1等位基因与儿童过敏性紫癜的相关研究.内蒙古医学报,2001,23(3)：154-156.
[8]Amoli MM,Thomson W,Hajeer AH,et al. HLA-DRB1*01 association with He-noch-Schoenlein purpura in patients from northwest spain. Jouanal of Rheumatolo-gy,2001,28:266-270.
[9]仝林虎,任少敏,郝富,等.蒙汉族儿童AP多器官损害与HLA-DRB1基因的关联性.中华微生物和免疫学杂志,2003,23(2)：136.
[10]任少敏,睢俊卿,仝林虎,等.内蒙籍汉族儿童AP关节胃肠道肾脏受累与HLA -DQA1的关联性[J].中华风湿病学杂志,2001,5(5)：295-298.
[11]傅亮,任少敏,仝林虎,等.内蒙地区汉族儿童HLA-DQA1基因对AP累及心脏的遗传易感性[J].中华心血管病杂志,2003,31(5)：360.
[12]仝林虎,任少敏,傅亮,等..内蒙地区汉族儿童AP器官系统损害与HLA-DRB1基因的关联性[J].中华微生物和免疫学杂志,2003,23(10)：780-781.
[1]胡亚美,江载芳,诸福棠实用儿科学.上册[M].第7版,北京：人民出版社,2002,688-690.
[2]Jesus M,Xose ML,Albert T,et al.Henoch-Schonlein purpura and IgA nephrop athy farther and son[J]Nephron,1990,54:77-79.
[3]Beck S,Geraghty D,Inoko H,et al. Complete sequence and gene map of a human major histocompatibility complex[J].Nature,1999,401(28):921.
[4]Willam E,Braun. HLA and diseas:A comprehensive review.New York:CRC press ,1979,1-27.
[5]The MHC Sequencing Consortium:Complete sequence and the gene map of a human major histocompatibility complex[J],Nature,1999,401-921.
[6]Petzl-Erler ML,Luz R,Sotomajor Vs.The HLA polymorphism of two distinctive south-American Indian triber:the Kaingang and the Guarani[J].Tissue Antigens, 1993,42:227.
[7]范丽安,周光炎.人类基因组MHC测序及其免疫学意义[T].上海免疫学杂志,1999,19(6)：321-322.
[8]Howell WM,Holgate ST. HLAgenetics and allergic disease[J],Thorax,1995, 50:815-818.
[9]陈慰峰主编.医学免疫学.北京：人民卫生出版社,2002,57-59.
[10]Jin DK,Kohsaka T,Koo JW,et al.Complement 4 loucs II genwe deletion and DQA1*0301 gene;genetic risk factors for IgA nephropathy and Henoch-Schone-lei nnephritis. Nephron,1996,73:390-395.
[11]韩秀萍,张志欣,肖毅等.北方汉族过敏性紫癜与HLA相关性研究.中华医学遗传学杂志,1997,142：79-82.
[12]Amoroso A,Berrino M,Canal L,et al. Immunogenetics of Henoch-Schoenlein disease.Eur J Immunogenet,1997,24:323-324.
[13]Amoli MM,Thomson W,Hajeer AH,et al. HLA-DRB1*01 association with Henoch-Schoenlein purpura in patients from northwest spain. Jouanal of Rheuma-tology,2001,28:266-270.
[14]任少敏仝林虎,锡林高娃等.内蒙古汉族儿童AP与DQA1基因的关联性研究[J],中华医学遗传学杂志,2002,19(1)：58.
[15]王晓娟,林娜,孟化等.HLA-DQA1等位基因与儿童AP的相关研究[J].内蒙古医学院学报,2001,23(3)：154.
[16]Dong KJ,Takao K,Ja WK,et al.Complement 4 locus II gene deletion and DQA1 *0301 gene:Genetic risk for IgA nephropatgy and Henoch-Schonlein nephritis [J]Nephron,1996,73:390-395.
[17]任少敏,杨光路,仝林虎等.蒙汉族儿童AP临床特点与HLA-DRB1基因关联性对比分析[J].中华风湿病学杂志,2003,7(8)：469..
[18]锡林高娃,张岸平,任少敏等.内蒙籍蒙古族儿童HLA-DRB1等位基因对过敏性紫癜的遗传易感性.中国基层医药,2002.9(11)：964-965.
[2]Beck S,Geraghty D,Inoko H,et al. Complete sequence and gene map of a human major histocompatibility complex[J].Nature,1999,401(28):921.
[3]任少敏,杨光路,仝林虎等.蒙汉族儿童AP临床特点与HLA-DRB1基因关联性对比分析[J].中华风湿病学杂志,2003,7(8)：469.
[4]Amoli MM,Thomson W,Hajeer AH,et al. HLA-B35 association with nephritis in Henoch-Schoenlein purpura[J].J Rheumatol.2002,29(5):948-949.
[5]胡亚美,江载芳,诸福棠实用儿科学.上册[M].第7版.北京：人民出版社,2002,688-690.
[6]谭建明,周永昌,唐孝达,主编.组织配型技术与临床应用[M].北京：人民卫生出版社,2002,317.
[7]王晓娟,林娜,孟化,等.HLA-DQA1等位基因与儿童过敏性紫癜的相关研究.内蒙古医学报,2001,23(3)：154-156.
[8]Amoli MM,Thomson W,Hajeer AH,et al. HLA-DRB1*01 association with He-noch-Schoenlein purpura in patients from northwest spain. Jouanal of Rheumatolo-gy,2001,28:266-270.
[9]仝林虎,任少敏,郝富,等.蒙汉族儿童AP多器官损害与HLA-DRB1基因的关联性.中华微生物和免疫学杂志,2003,23(2)：136.
[10]任少敏,睢俊卿,仝林虎,等.内蒙籍汉族儿童AP关节胃肠道肾脏受累与HLA -DQA1的关联性[J].中华风湿病学杂志,2001,5(5)：295-298.
[11]傅亮,任少敏,仝林虎,等.内蒙地区汉族儿童HLA-DQA1基因对AP累及心脏的遗传易感性[J].中华心血管病杂志,2003,31(5)：360.
[12]仝林虎,任少敏,傅亮,等..内蒙地区汉族儿童AP器官系统损害与HLA-DRB1基因的关联性[J].中华微生物和免疫学杂志,2003,23(10)：780-781.
[1]胡亚美,江载芳,诸福棠实用儿科学.上册[M].第7版,北京：人民出版社,2002,688-690.
[2]Jesus M,Xose ML,Albert T,et al.Henoch-Schonlein purpura and IgA nephrop athy farther and son[J]Nephron,1990,54:77-79.
[3]Beck S,Geraghty D,Inoko H,et al. Complete sequence and gene map of a human major histocompatibility complex[J].Nature,1999,401(28):921.
[4]Willam E,Braun. HLA and diseas:A comprehensive review.New York:CRC press ,1979,1-27.
[5]The MHC Sequencing Consortium:Complete sequence and the gene map of a human major histocompatibility complex[J],Nature,1999,401-921.
[6]Petzl-Erler ML,Luz R,Sotomajor Vs.The HLA polymorphism of two distinctive south-American Indian triber:the Kaingang and the Guarani[J].Tissue Antigens, 1993,42:227.
[7]范丽安,周光炎.人类基因组MHC测序及其免疫学意义[T].上海免疫学杂志,1999,19(6)：321-322.
[8]Howell WM,Holgate ST. HLAgenetics and allergic disease[J],Thorax,1995, 50:815-818.
[9]陈慰峰主编.医学免疫学.北京：人民卫生出版社,2002,57-59.
[10]Jin DK,Kohsaka T,Koo JW,et al.Complement 4 loucs II genwe deletion and DQA1*0301 gene;genetic risk factors for IgA nephropathy and Henoch-Schone-lei nnephritis. Nephron,1996,73:390-395.
[11]韩秀萍,张志欣,肖毅等.北方汉族过敏性紫癜与HLA相关性研究.中华医学遗传学杂志,1997,142：79-82.
[12]Amoroso A,Berrino M,Canal L,et al. Immunogenetics of Henoch-Schoenlein disease.Eur J Immunogenet,1997,24:323-324.
[13]Amoli MM,Thomson W,Hajeer AH,et al. HLA-DRB1*01 association with Henoch-Schoenlein purpura in patients from northwest spain. Jouanal of Rheuma-tology,2001,28:266-270.
[14]任少敏仝林虎,锡林高娃等.内蒙古汉族儿童AP与DQA1基因的关联性研究[J],中华医学遗传学杂志,2002,19(1)：58.
[15]王晓娟,林娜,孟化等.HLA-DQA1等位基因与儿童AP的相关研究[J].内蒙古医学院学报,2001,23(3)：154.
[16]Dong KJ,Takao K,Ja WK,et al.Complement 4 locus II gene deletion and DQA1 *0301 gene:Genetic risk for IgA nephropatgy and Henoch-Schonlein nephritis [J]Nephron,1996,73:390-395.
[17]任少敏,杨光路,仝林虎等.蒙汉族儿童AP临床特点与HLA-DRB1基因关联性对比分析[J].中华风湿病学杂志,2003,7(8)：469..
[18]锡林高娃,张岸平,任少敏等.内蒙籍蒙古族儿童HLA-DRB1等位基因对过敏性紫癜的遗传易感性.中国基层医药,2002.9(11)：964-965.