灰飞虱体内水稻条纹病毒的检测及传毒相关蛋白的研究
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摘要
水稻条纹病毒(Rice stripe virus,Rsv)是纤细病毒属(Tenuivirus)的典型成员,由灰飞虱以持久方式传播(Laodelphax striatellus Fallen,),曾分布于我国16个省市,至今仍在部分省市暴发流行,给我国水稻生产造成了严重损失。本文利用已经制备的多克隆抗体SV21和单克隆抗体389,应用多孔板间接ELISA、DIBA和Western blotting方法都能检测出单头灰飞虱体内水稻条纹病毒,检测灵敏度以多孔板间接ELISA最高,其次为DIBA,Western最低,用单克隆抗体检测灵敏度高于多克隆抗体。RT-PCR、IC-RT-PCR和DB-RT-PCR 3种方法中,RT-PCR对单头灰飞虱稀释到400倍都可以检测到病毒;IC-RT-PCR在多克隆抗体SV21稀释浓度大于500倍就检测不出RSV,单克隆抗体稀释浓度大于800倍也检测不到RSV;DB-RT-PCR结果显示单头灰飞虱在稀释400倍后都无阳性反应。
检测灰飞虱体内由水稻条纹病毒(RSV)编码的外壳蛋白CP、病害特异性蛋白SP,以及非结构蛋白NS2、NS3和NSvc4 5种蛋白以及病毒粒子(CP)与SP、NS2、NS3、NSvc4的体外结合,为进一步研究水稻条纹病毒与其介体昆虫互作提供有用信息。本文提取了高带毒灰飞虱(Q家系)总蛋白,用免疫方法检测了昆虫体内CP、SP、NS2、NS3和NSvc45种重要蛋白的表达以及病毒粒子(CP)与SP、NS2、NS3、NSvc4的体外结合情况。结果表明在带毒灰飞虱体内以上5种蛋白都能检测到,而且CP含量最高,NSvc4、SP、NS3、NS2含量依次降低;用体外原核表达后纯化的蛋白进行了RSV粒子(CP)与SP、NS2、NS3、NSvc4的体外结合情况实验,实验结果表明病毒粒子(CP)与sP、NSvc4之间存在体外结合关系。检测结果为灰飞虱作为RSV的昆虫寄主并且可以在其体内增殖的结论提供了支持,表达差异表明病毒编码蛋白在介体灰飞虱体内可能存在着一定的协调关系;CP与SP的结合为前人关于CP与SP可能共同作用于叶绿体进而影响症状的严重度的报道又提供了一个新的证据,CP与NSvc4之间存在体外结合关系,也为NSvc4可能作为运动蛋白诱导病毒在胞间运动需要CP的辅助提供了佐证。
为了获取灰飞虱体内RSV受体蛋白的情况。本文以Bio-Rad高通量双向电泳系统为技术平台,通过改进和优化灰飞虱蛋白的提取方法及电泳条件,建立了适合灰飞虱总蛋白的高通量、高分辨率和高重复性的双向电泳技术体系。在灰飞虱蛋白双向电泳图谱上能检测到至少54个蛋白点。应用病毒覆盖检测技术,我们从高亲和性无毒灰飞虱蛋白质中筛选出一个大小约为60KD,等电点约为6.7的蛋白,该蛋白可以与RSV在体外发生特异性结合。
Rice stripe virus (RSV), a typical member of Tenuivirus, is one of important rice viruses in Easten Asian. It had inflicated damage over a huge hectarage from 2000 in Jiangsu province and its neighor areas. Polyclonal antibody SV21 and monoclonal antibody 3B9 were used in the paper. RSV in a single small brown plant hopper (Laodelphax striatellus Fallén, SBPH) could be detected with the ELISA, DIBA and Western blotting methods successfully. Results showed that the monoclonal antibody was more sensitive than polyclonal antibody and polywell-plate indirect-ELISA with polyclonal antibody or monoclonal antibody as testing antibody had a higher specificity and sensitivity than DIBA and Western blotting. Among the three molecular detection methods, RSV could be detected with 1:400 dilution of a single SBPH using RT-PCR, however, RSV could not be detected using IC-RT-PCR if the dilution of polyclonal antibody SV21 was less than 1:500 or the dilution of monoclonal antibody was less than 1:800. The results of DB-RT-PCR also showed that RSV could not be detected with less than 1:400 dilution of a single male or female SBPH.
Detecting the five proteins encoded by RSV (CP, SP, NS2, NS3 and NSvc4) in SBPH, investigating the interactions between virion (CP) and the other four proteins of RSV in vitro, and providing useful data for further studying relationship between RSV and its insect vector. The total protein of virus infected SBPH was extracted, and hybrided with specific antibodies of the five RSV proteins. The gel overlay assay was performed. All the five proteins encoded by RSV presented in the SBPH. Further more, CP has a higher density among the five proteins. Besides, results indicated that there are interactions with virion (CP) and SP or virion (CP) and NSvc4 in vitro. Our study confirmed that RSV replicates in SBPH, and different concentration of the five proteins presented in SBPH body indicated the different roles in its replication. The interaction in vitro between CP and NSvc4 (the potential movement protein) indicates that the cell-to-cell movement of RSV may depend on this relationship between CP and NSvc4. Our results also showed the interaction between CP and SP, which has been reported previously on chloroplast of rice and influence to the severity of symptoms.
In order to parse the case of receptor protein of RSV in SBPH . In this dissertation, through optimizing the protein of SBPH extraction methods and electrophoresis conditions, a large-scale, high- resolution and reproducible two-dimensional electrophoresis(2-DE) analysis system was established based on the technical platform of Bio-Rad large-scale 2- DE system. At least 54 protein pots were detected on the gels of SBPH.The protein of Mr= 60 KD, pI=6.7 was detected by virus overlay assay (VOA) from SBPH of non-infected and high-affinity. There is interaction with the protein and RSV in vitro, specially.
检测灰飞虱体内由水稻条纹病毒(RSV)编码的外壳蛋白CP、病害特异性蛋白SP,以及非结构蛋白NS2、NS3和NSvc4 5种蛋白以及病毒粒子(CP)与SP、NS2、NS3、NSvc4的体外结合,为进一步研究水稻条纹病毒与其介体昆虫互作提供有用信息。本文提取了高带毒灰飞虱(Q家系)总蛋白,用免疫方法检测了昆虫体内CP、SP、NS2、NS3和NSvc45种重要蛋白的表达以及病毒粒子(CP)与SP、NS2、NS3、NSvc4的体外结合情况。结果表明在带毒灰飞虱体内以上5种蛋白都能检测到,而且CP含量最高,NSvc4、SP、NS3、NS2含量依次降低;用体外原核表达后纯化的蛋白进行了RSV粒子(CP)与SP、NS2、NS3、NSvc4的体外结合情况实验,实验结果表明病毒粒子(CP)与sP、NSvc4之间存在体外结合关系。检测结果为灰飞虱作为RSV的昆虫寄主并且可以在其体内增殖的结论提供了支持,表达差异表明病毒编码蛋白在介体灰飞虱体内可能存在着一定的协调关系;CP与SP的结合为前人关于CP与SP可能共同作用于叶绿体进而影响症状的严重度的报道又提供了一个新的证据,CP与NSvc4之间存在体外结合关系,也为NSvc4可能作为运动蛋白诱导病毒在胞间运动需要CP的辅助提供了佐证。
为了获取灰飞虱体内RSV受体蛋白的情况。本文以Bio-Rad高通量双向电泳系统为技术平台,通过改进和优化灰飞虱蛋白的提取方法及电泳条件,建立了适合灰飞虱总蛋白的高通量、高分辨率和高重复性的双向电泳技术体系。在灰飞虱蛋白双向电泳图谱上能检测到至少54个蛋白点。应用病毒覆盖检测技术,我们从高亲和性无毒灰飞虱蛋白质中筛选出一个大小约为60KD,等电点约为6.7的蛋白,该蛋白可以与RSV在体外发生特异性结合。
Rice stripe virus (RSV), a typical member of Tenuivirus, is one of important rice viruses in Easten Asian. It had inflicated damage over a huge hectarage from 2000 in Jiangsu province and its neighor areas. Polyclonal antibody SV21 and monoclonal antibody 3B9 were used in the paper. RSV in a single small brown plant hopper (Laodelphax striatellus Fallén, SBPH) could be detected with the ELISA, DIBA and Western blotting methods successfully. Results showed that the monoclonal antibody was more sensitive than polyclonal antibody and polywell-plate indirect-ELISA with polyclonal antibody or monoclonal antibody as testing antibody had a higher specificity and sensitivity than DIBA and Western blotting. Among the three molecular detection methods, RSV could be detected with 1:400 dilution of a single SBPH using RT-PCR, however, RSV could not be detected using IC-RT-PCR if the dilution of polyclonal antibody SV21 was less than 1:500 or the dilution of monoclonal antibody was less than 1:800. The results of DB-RT-PCR also showed that RSV could not be detected with less than 1:400 dilution of a single male or female SBPH.
Detecting the five proteins encoded by RSV (CP, SP, NS2, NS3 and NSvc4) in SBPH, investigating the interactions between virion (CP) and the other four proteins of RSV in vitro, and providing useful data for further studying relationship between RSV and its insect vector. The total protein of virus infected SBPH was extracted, and hybrided with specific antibodies of the five RSV proteins. The gel overlay assay was performed. All the five proteins encoded by RSV presented in the SBPH. Further more, CP has a higher density among the five proteins. Besides, results indicated that there are interactions with virion (CP) and SP or virion (CP) and NSvc4 in vitro. Our study confirmed that RSV replicates in SBPH, and different concentration of the five proteins presented in SBPH body indicated the different roles in its replication. The interaction in vitro between CP and NSvc4 (the potential movement protein) indicates that the cell-to-cell movement of RSV may depend on this relationship between CP and NSvc4. Our results also showed the interaction between CP and SP, which has been reported previously on chloroplast of rice and influence to the severity of symptoms.
In order to parse the case of receptor protein of RSV in SBPH . In this dissertation, through optimizing the protein of SBPH extraction methods and electrophoresis conditions, a large-scale, high- resolution and reproducible two-dimensional electrophoresis(2-DE) analysis system was established based on the technical platform of Bio-Rad large-scale 2- DE system. At least 54 protein pots were detected on the gels of SBPH.The protein of Mr= 60 KD, pI=6.7 was detected by virus overlay assay (VOA) from SBPH of non-infected and high-affinity. There is interaction with the protein and RSV in vitro, specially.
引文
1.Anzola JV,Z.K.Xu,T.Asamizu,et al.Segment-specificinverted repeats found adjacent to conserved terminal sequences in wound tumor virus genome and defective interfering RNAs[J].Proc.NatlAcad.Sci USA,1987,84(23):8301-8305.
2.Aranda M,Fraile A,Dopazo J,et al.Contribution of mutation and RNA recombination to the evolution of a plantpathogenic RNA[J].Journal of Molecular Evolution,1997,(1):81-88.
3.Areya C D,Atreya P L,Thornbury D W et al.Site-directed mutation in potyvirus HC-Pro gene affect helper component acticity cirus accumulation and symptom express in infected tobacco plant [J].Virology,1992,191:106-111.
4.Atreya CD,Raccob B,Pirone T P.Apoint mutation in coat protein abolishes aphid transmissibility of Potyvirus[J].Virology,1990,178:161-165.
5.Bandla MD,L.Campbell,D.E.Ulhnan,et al.Interaction of tomato spotted wilt tospovirus.(TSWV)glycoproteins with a thrips midgut protein,a potential cellular receptor for.TSWV[J].Phytopathol.,1998.88:98-104.
6.Barbier P,Takashi M,Nakamura I,et al.Solubilization and promoter analysis of RNA polymerase from rice stripe virus[J].Journal of Virology,1992,66(10):6171-6174.
7.Boonham N,Welsh K,Smith P.Detection of Potatoviurses using microarray technology:towards a genetic method for Plant viral disease diagnosis[J].Virol Methods,2003,108:181-187.
8.Brunt A.A,K.Cuabtree,M.J.Dallwitz et al.Sweet potato sunken vein clostero virus[M].Viruses of plants,CAB International,Cambrige,United Kingdom.1996.
9.Cai L,Ma X,Kang,et al.Detecting rice stripe virus(RSV) in the small brown planthopper with high specificity by RT-PCR[J].Journal of Virological Methods,2003,112:115-120.
10.Chay C A,Gunasinge UB,Dinesh-kumar S P et al.Aphid transmission and systemic plant infection determinants of barley yellow dwarf Luteovirus-PAV are contained in the coat protein readthrough domain and 17 kDa protein respectively[J].Virology.,1996,219:57-65.
11.Chen,C,Ko W.Studies on the time of rice stripe virus infection and field experiments on disease control.Res[J].Bull.Taichung District Agric.Improv.Sta.(Taiwan) 1986,12:51-59.
12.Chomchan P,Li S,Miranda G,et al.Interactions among proteins coded on rice grassy stunt virus genome.[J].Annuals of phytopathological society of Japan,2000,66:164.
13.DharA.K,SinghR.P.Improvement in the sensitivity of PVYN detection by increasing the cDNA Probe size.Journal of virological method,1994,50(1-3):197-210.
14.Dohi K,Mise K,Furusawa I,et al.RNA-dependent RNA polymerase complexs of Brome mosaic virus:analysis of the molecular structure with monoclonal antibodies[J].Journal of General Virology,2002,83:2879-2890.
15.Dorig R E,Marcil A,Chopra A,et al.The human CD46 molecule is a receptor for measles virus (Edmonston)[J].Cell,1993,75(2):295-305.
16.Elliot R.Molecular biology of the Bunyaviridae[J].Journal of General virology,1990,71:501-522.
17.F.奥斯伯,R.E.金斯顿,J.G.塞德曼,等.1998精编分子生物学实验指南[M],科学出版社.
18.Falk B,Tsai J.Biology and molecular biology of viruses in the genus Tenuivirus[J].Annual Review of Phytopathology,1998,36:139-163.
19.Fukamachi S,Inoe H.Period of infection by rice stripe virus during normal season rice culture in Kagoshima Prefecture[C].Proceeding of the Association for Plant Protection of Kyushu,1989,35:3-4.
20.Galon A.Antiguns Y.Rosher A et al.A zucchini yellow mosaic virus coat Protein gene mutation restores aphid transmissibility but has no effect on multiplication[J].Gen Virology,1992,73:2183-2187.
21.Garret A,Kerlan C,Thomas D..Visualization of a luteovirus in the vector aphid's body by two gold immunolabelling techniques:a comparative study.Journal of virological methods,1991,35(1):93-107.
22.Gergerich RC,H.A.Scott.Determinants in the specificity of virus transmission by leaf-feeding beetles[J].Adv.Dis.vector Res,1991.8:1-14.
23.Gildow F E,Gray S M.The aphid salivary gland lamma basal as a selective barrier associated with vector specific transmission of Barley yellow dwarf leteovirus[J].Phytophathology,1993,83:1293-1302.
24.Gingery R,Nault L,Yamashita S.Relationship between maize stripe virus and rice stripe virus[J].Journal of General Virology,1983,64(3):1765-1770.
25.Govier DA,Kassanis B.Evidence that a component other than the virus particles is neel for aphid transmission of Potato VirusY[J].Virology,1974,57:285-286.
26.Granier F.,Durand-Tardif M.,Casse-Delbart F,et al.Mutation in zucchini yellow mosaic virus HC protein associated with loss of aphid transmissibility[J].Gen Virology,1993,74:2737-2742.
27.Gray SM,N Banerjee.Mechanisms of arthropod transmission of plant and animal viruses[J].Microbiology and Molecular Biology Reviews,1999,63(1):128-148.
28.Gray SM.Plant virus proteins involved in natural vector transmission[J].Trends.Microbid.,1996,4:253-294.
29.Guilley H.,WiptS C.,RichardsK et al.Mucleotide sepuence of cucurbit aphid- bione yellow luteovirus[J].Virology,1994,202:1012-1017.
30. Hamamatsu C, Ishihama A. Ambisense RNA genome of rice stripe virus[J]. Uirusu, 1994, 44(1):19-25.
31. Hamamatsu C, Toriyama S, Toyoda T, et al. Ambisense coding strategy of the rice stripe virus genome: in vitro translation studies[J].J. Gen. Virol, 1993; 74: 1125-1131.
32. Hataya I ,Inoue A.K., Shikata E. PCR-microplate hybridization, method for plant virus detection.Journal of virologicalMehtods, 199A, 46 (2) :223-236.
33. Hayakawa T, Zhu Y, Itoh K, et al. Genetically engineered rice resistant to rice stripe virus, an insect-transmitted virus[J]. Proceeding of the National Academy of Science. USA, 1992, 89:9865-9869.
34. Hayano Y, Kakutani T, Hayashi T, et al. Coding strategy of rice stripe virus: major nonstructural protein is encoded in viral RNA segment 4 and coat protein in RNA complementary to segment 3 [J]. Virology. 1990, 177: 372-374.
35. Hayano-Saito Y, Tsuji T, Fujii K, et al. Localization of the rice stripe disease resistance gene, Stvb-I : by graphical genotyping and linkage analyses with molecular markers [J]. Theoretical and Applied Genetics, 1998,96(8): 1044-1049.
36. Hayashi T, Usugi T, Nakano M, et al. On the strains of rice stripe virus(1): an attempt to detect strains by difference of molecular size of disease-specific proteins[J]. Proceeding of the Association for Plant Protection of Kyushu, 1989,35(1): 1-2.
37. Hibino H. Biology and Epidemiology of rice viruses[J]. Annual Review of Phytopathology, 1996, 34: 249-174.
38. Holland J, Spindler K, Horodyshi F. Rapid evolution of RNA genomes[J]. Science, 1982, 215: 1577-1585.
39. Hong S S, Karayan L,Tournier J,et al.A denovirus type 5 fiber konb bingds to MHC class 1α2 domain at the surface of human epithelial and B lymphoblastoid cells [J]. EMBO, 1997,16(9):2294-2306.
40. Ishihama H. Nucleotide sequence and rice stripe virus RNA segment 2[J]. Journal of General Virology, 1993, 74(4): 769-733.
41. Ishii M, Ono K. On strains of rice stripe virus[J]. Annuals of the phytopathological society of Japan,1967, 32(1): 83.
42. Ito K, Okada M. Bionomics of the small brown planthopper in relation to the transmission of rice stripe virus[J]. Plant Protection (Japan), 1985, 39(11): 525-53.
43. Kakutani T, Hayano Y, Hayashi T, et al. Ambisense segment 3 of rice stripe virus: the first instance of a virus containing two ambisense segments[J]. Journal of General Virology, 1991, 72: 465-468.
44. Kakutani T, Hayashi T, Minobe Y. Ambisense segment 3 of rice stripe virus: the first instance of a virus containing two ambisense segments[J].Journal of General,1991,72(2):465-4.68.
45.Kisimoto R,Yamada Y.Present Status of Contolling Rice Stripe Virus[C].Edited by Hadidi A,Khetarpal R and Oganezawa H.Chapter 35:470-483.
46.Kisimoto R,Yamada Y.Present Status of Controlling Rice Stripe Virus[A].In:Hadidi A,Khetarpal RK,Koganezawa H(ed).Plant Virus Disease Control[C].St.Paul.Minnesota:APS Press,1991:470-481.
47.Kisimoto R.Ecology office stripe disease[J].Hereditas(In Japan),1972,(12):34-40.
48.Kisimoto R.Genetic variation in the ability of a planthopper vector,Laodelphaxstriatelllus Fallen to acquire the rice stfie virus[J].Virology,1967,32(1):144-152.
49.Kiso H,Yamamoto T.Infection and symptom development in rice stripe diseases,with special reference to disease specific protein other than virus.Rev[J].Plant Protect.Res.(Japan) 1973,6:75-100.
50.Lee G P,Min BE,Kim CS.Plant virus cDNA chip hybridization for detection and dieffrentiation of four cucurbit-infecting Tobamoviruss[J].Virol Methods,2003,110:19-24.
51.Li W H,Moore M J,Vasilieva N,et al.Angiotensin-converting enzyme 2 is a functional receptor for SARS coronavims[J].Nature,2003,426(6965):450-454.
52.Liang D,Ma X,Qu Z.PVC2 of rice stripe tenuivirus is a component of fibrillar electron-opaque inclusion body.Abstract of 19~(th) American Society for Virology[M],Colorado,USA,2000,7:15-19.
53.Lin Hanxin,Wei Taiyun,Wu Zujian,et al.Molecular variability in coat protein and disease-specific protein genes among isolates of rice stripe virus in China[M].Abstract for the ⅪInternational Congress of Virology,Australia,Sydney,1999:235-236.
54.ltamirez B,Haenni A.Molecular biology of tenuivinrses:a remarkable group of plant vinise[J].Jouranl of General Yrology,1994,75:467-475.
55.Ludwig G V,Israel B A,Christensen B M,et al.Role of La crosse virus glycoproteins in attachment of virus to host cell[J].Virology,1991,181:564-571.
56.Marjolein Kikkert,Cor Meurs,Fennet van de Wetering,Simone Dorfm(u|¨)ller,Dick Peters,Richard Kormelink,and Rob Goldbach.Binding of tomato spotted wilt vires to a 94-kDa thrips protein.Phytopathology,1998,88:63-69.
57.Medeiros RB,D E Ullman,J.L.Sherwood,et al.Immunoprecipitation of a 50 kDa protein:a candidate receptor component for tomato spotted wilt tospovirus in its thrips vector[J].Virus Research,2000,67(2):109-118
58.Morris P.C.著,朱传楹译.生物防治与植物保护.国外农学-植物保护,1992,5(4):44-46.
59.Nishiguchi M,Mori M,Suzuki F.Specific detection of a severe strain of sweet potato feathery mottle virus(SPFMV-S) by reverse transcription and polymerase chain reaction(RT-PCR)[J]. Annuals of the phytopathological society of Japan, 1995, 61:119-122.
60. Nolasco G, de Bias C, Torres V, Ponz F. A method combining immunocapture and PCR amplification in a microtiter plate for the detection of plant virus and subviral pathogens. Journal of virological Methods, 1993.,45(2):201-218.
61. Noris E,A.MVaira,P.Caciagli,V. Masenga,et al. Amino acids in the capsid protein of tomato yellow leaf curl virus that are crucial for systemic, infection, particle formation, and insect transmission [J].Virology, 1998,72(12): 10050-10057.
62. Nuss DL, D. Summers. Variant dsRNAs associated with transmission-defective isolates of wound tumor virus represent terminally conserved remnants of genome segments [J]. Virology, 1984, 133(2): 276-288.
63. Okuyama S, Asuyama H. The propagation of the rice stripe virus in the body of the vector[C], in: Programs and Abstracts of the Symposium on Vectors of Plant Viruses, 1962, 288: 10.
64. Omura T, Hibino H, Usugi T. Detection of rice viruses in plants and individual insect vectors by Latex Flocculation Test[J]. Plant Diseases , 1984, 68: 374-378.
65. Omura T, J, Yan, B. Zhong, et al. The P2 protein of rice dwarf phytoreovirus is required for adsorption of the virus to cells of the insect vector [J]. Virology, 1998, 72(11): 9370-9373.
66. Omura T, Takahashi Y, Shohara K, et al. Production of monoclonal antibodies against rice stripe virus for the detection of virus antigen in infected plants and viruliferous insects[J]. Annals of the phytopathological society of Japan, 1986a, 52(2): 270-277.
67. Omura T, W. Maruyama, et al. Involvement in virus infection to insect vector cells of the P2 outer capsid proteins of rice gall dwarf and rice dwarf phytoreoviruses [J]. Phytopathol, 1997, 87 : S72.
68. Peiffer H L., Glidow F E., Gray S M. Two distinct mechanisms regulate luteovirus transmission efficiency and specificity at the aphid salivary gland. [J] Gen Virology, 1997,78: 495-503.
69. Pirone T P. Viral genes and gene products that determine insect transmissibility [J]. Seminar in Virology, 1991,2:81-87.
70. Pirone T P., Blanc S. Helper-dependent transmission of plant viruses [J]. Annu Rev Phytopathol, 1996, 34: 227-247.
71. Qiu W, Geske S, Hichey C. Tomato spotted reassortrnent and genome segment-specific adaptation[J]. Urology, wilt, 1998, 244: 186-194.
72. Rajcani J. Molecular mechanisms of virus spread and virion components as tools of virulence [J].Acta Microbiol Immunol Hung, 2003, 50(4): 407-443.
73. Ramirez B, Haenni A. Molecular biology of tenuiviruses, a remarkable group of plant viruses[J].Journal of General Virology, 1994, 75: 467-475.
74. Ramirez B, Lozano I, Constantino L, et al. Complete nucleotide sequence and coding strategy of rice hoja blanca virus RNA4[J].Journal of General Virology,1993,74:2463-2468.
75.Resende R.,de Haan P,de Arila AC,et al.Defective isolates of tomato spotted wilt virus.Virus-Thrips-Plant Interactions of Tomato Spotted Wilt Virus[J].Virology,1991.72:2375-2383.
76.Robinson D.J.,Romero J..Sensitivity and specificity of nucleic acid probes for potato leafrollluteovirus detection.Journal of virological Methods,1991,34(2):209-219.
77.Ryu C J,Cho D Y,Gripon P,et al.An 80-kilodalton protein that binds to the Pre-Sldomain of hepatitis B virus[J].Virology,2000,74(1):110-116.
78.Salas-Benito J S,Del-Angel R M.Identification of two surface proteins from C6/36 cells that bind Dengue type 4 virus[J].Virology,1997,71(10):7246-7252.
79.Shinkai A.Studies on insect transmission of rice virus diseases in Japan[J].Bull.Natl.Instit.Agric.Sci.Series C 1962,14:104-112.
80.Singh R.R,Kutz J,Boiteau G.Detection of stylet-borne and circulative potato viruses in aphids by duplex reverse transcription polymerase chain reaction.Journal of virological Methods,1996,59(1-2):189-196.
81.Takahashi M,Toriyama S,Kikuchi Y,Hayakawa T,Ishihama A.Complementarity between the 5'-and 3'-terminal sequences of rice stripe virus RNAs[J].Journal of General virology,1990,71:2817-2821.
82.Takahashi M,Toriyama S,Hamamatsu C,Ishihama A.Nucleotide sequence and possible ambisense coding strategy of rice stripe virus RNA segment 2.Journal of General Virology,1993,74(4):769-763.
83.Takahashi M.Expression of rice stripe virus 22.8k protein in insect cells[J].Annuals of the phytopathlogical society of Japan,1998,65(3):342.
84.Thomas C.Mettenleiter Brief overview on cellular virus receptors[J].Virus Research,2002,82:3-8.
85.Thornhbury T P.Comparative sequence of helper component(HC) region of PVY and HC-defective strain potato virus C[J].virology,1990,178:573-578.
86.Tomaro,M.W.Maruyama,A.Kiknche.Protein P2 results in nontransmissibility by the insect vector of rice dwarf phytoreovims[J].Virology,1997,71:8019-8023.
87.Toriyama S,Takahashi M,Sano Y,Shimizu T,Ishihama A..Nucleotide sequence of RNA1 the largest genomic segment of rice stripe virus,the prototype of the tenuiviruses[J].Journal of General Virology,1994,75:3569-3579.
88.Toriyama S.Characterization of rice stripe virus:a heavy component carrying infectivity[J].Journal of General Virology,1982,61:187-195.
89.Toriyama S.Rice stripe virus[J].CMI/AAB Descriptions of Plant Virus,1983,No:269.
90.Toriyama S.Rice stripe virus:prototype of a new group of viruses that replicated in plants and insects[J].Microbllogical Sciences,1986,3:347-351.
91.Toriyama S.Rice stripe virus[C].CMI/ABB.Desccription of plant viruses.1983,269:15.
92.Walsh K,North J,Barker I.,Boonham N.Detection of different strains of Potato virus Y and their mixed infections using competitive fluorescent RT-PCR.Journal of virological Methods,2001,91(2):167-173.
93.Wang J Y.,Chay F E.,Gray S M.Readthrough protein associated with cirions of barley yellow dwarf leuteovirus and its potential role in regulation the efficiency of aphid transmission[J].Virology,1995,206:954-962.
94.Welnicki M,Zekanowski C,Zagorski W.Digoxigenin-labelled molecular probe for the simultaneous detection of three potato pathogens:potato spindle tuber viroid(PSTVd),potato virus Y(PVY),and potato leaf roll virus(P LRV).Acta Biochim Pol,1994,41(4):473-475.
95.White M A.The yeast two-hybrid system:forward and reverse[J].Proc Natl Acad Sci USA,1996,93(19):10001-10003.
96.Wijkamp I.,van Lent J.,Kormelink R.,et al.Multiplication of tomato spotted wolt virus in its insect vector,Frankliniella occidentals[J].Gen Virology,1993,74:341-349.
97.Yamada W,Shiomi T,Yamamoto H.Studies on the stripe disease of rice plant,Ⅱ.On the disease occurrence in Okayama Prefecture and its control[J].Okayama Pref.Agric.1955,52:113-124.
98.Yah J.M.Tomaru,A.Takahachi,et al.P2 protein encoded by genome segment S2 of rice dwarf phytoreovirus is essential for vires infection[J].Virology,1996,224:539-541.
99.Yasuo S,Ishii M,Yamaguchi T.Studies on rice stripe disease Ⅰ.Epidemiological and ecological studies on rice stripe disease in the Kanto-Tosan district of central part of Japan[M].Review of Plant Protection Research,1968,1:96-104.
100.Zhang H,Wu R.Effcient regenerationof transgenic plant from rice protoplasts and correctly regulated expression of the foreign gene in the plant[J].Theor Appl Genet,1988,76:835-840.
101.Zhu Y,Hayajawa T,Toriyama S.Compete nucleotide sequence of RNA4 of rice stripe virus isolate T and comparison with anther isolate and with maize stripe virus[J].Journal of General lrrology,1992,73:1309-1312.
102.Zhu Y,Hayakawa T,Toriyama S,Takahashi M.Complete nucleotide sequence of RNA3 of rice stripe virus:an ambisense coding strategy[J].Journal of General Virology,1991,72:763-767.
103.白艳菊,李学湛,吕典秋.应用DAS-ELISA法同时检测马铃薯病毒[J].中国马铃薯,2000,4(3):143-44
104.陈爱香.水稻条纹病毒NS2基因的分子变异及其原核表达[D],2004,福州:福建农林大学.
105.陈光育,龚林根,秦维勒,傅华欣.酶联法在水稻条纹叶枯病流行预测上的应用[J].植物保护学 报,1991,18(1):49-52.
106.陈光育.酶联免疫吸附试验检测水稻条纹叶枯病介体昆虫带毒率[J].植物保护学报,1984,11(2):73-78.
107.陈京,胡伟贞,张成良,宋淑敏,王树琴.番茄环斑病毒单克隆抗体细胞株的建立及检测方法的研究[J].植物检疫,1994,8(1):1.
108.陈庆忠,柯文华.水稻条纹叶枯病主要感染时期之推定及适期防治试验[J].台中区农业改良场研究简报,1986,12:51-60.
109.陈庆忠,柯文华.台湾水稻条纹叶枯病流行学研究[J].植物保护会刊,1989,31(3):290-303.
110.陈声祥.水稻病毒发生现状及研究进展[J].浙江农业科学,1996,(1):41-42.
111.程兆榜,杨荣明,周益军,等.江苏稻区水稻条纹叶枯病发生新规律[J].江苏农业科学,2002(1):39-41
112.丁天兵,任君萍,马文煜.毒受体的研究方法[J].中国病毒学,2006,21(2):198-193
113.高东明,秦文胜.抗性品种对预防水稻条纹叶枯病发生和流行的研究[J].浙江农业学报,1994,6(3):141-149.
114.高苓昌,宋克勤,张洪瑞,等.黄淮稻区水稻条纹叶枯病的发病特点与综合防治[J].山东农业科学,2006(3):66-67.
115.弓利英,夏立,马丽,等.沿黄稻区水稻条纹叶枯病的发生及防治技术[J].河南农业科学,2006(7):64-65.
116.贺鹏飞.PLRV的IC-RT-PCR和DAS-ELlsA两种检测方法的比较[D].湖南农业大学学士学位论文,1999,12-13.
117.贾慧.基因芯片技术在百合病毒检测中的应用[D].河北农业大学硕士学位论文,2004.
118.蒋耀培,李建刚.上海地区水稻条纹叶枯病的发生与防治技术初探[J].上海农业科技,2005(5):37-38.
119.鞠振林,朱笑梅,薛爱红.改进的直接组织斑点免疫测定法在植物病毒及细菌检测中的应用[J].植物病理学报,1993,23(4):366-371.
120.李汝刚,郭军,鞠振林,朱笑梅,梁华,王远程.马铃薯x、Y病毒的单克隆抗体及其在马铃薯病毒检测上的应用[J].马铃薯杂志,1992,6(4):233-236.
121.李毅,陈章良.植物病毒胞间运动的分子生物学和细胞学研究进展[J].植物生理学通讯,1995,31(6):463-469.
122.林付根,成长庚,赵阳.水稻条纹叶枯病治虫防病策略的实践体会[J].中国植保导刊,2006(1):13-15.
123.林含新,林奇英,谢联辉.水稻条纹病毒分子生物学研究进展[J].中国病毒学报,1997,12(3):203-209.
124.林含新,魏太云,吴祖建,等.我国水稻条纹病毒7个分离物的致病性和和化学特性比[J].福建农 业大学学报,2002,31(2):164-167.
125.林含新,魏太云,吴祖建,等.我国水稻条纹病毒一个强致病性分离物的RNA4序列测定与分析[J].微生物学报,2001,41(1):25-30.
126.林含新,魏太云,吴祖建,等.应用PCR-SSCP技术快速检测我国水稻条纹叶枯病毒的分子变异[J].中国病毒学,2001,16(2):166-169.
127.林含新,吴祖建,林奇英,等.水稻品种对水稻条纹病毒的抗性鉴定及其作用机制研究初报[M].植物病毒与病毒病防治(刘仪主编),中国农业科技出版社,北京,1997:p188-192.
128.林均安,高锦梁,洪健主编.实用生物电子显微技术[M].沈阳:辽宁科学技术出版社.1988.
129.林奇田,林含新,吴祖建,林奇英,谢联辉.水稻条纹病毒外壳蛋白和病害特异性蛋白在寄主体内的积累[J].福建农业大学学报,1998,27(3):322-326.
130.林奇英,谢联辉,周仲驹,谢莉妍,吴祖建.水稻条纹叶枯病的研究Ⅰ.病害的分布和损失[J].福建农学院学报,1990,19(4):421-425.
131.刘德钧,韩长安,黄拔山,王金其,陈建生,朱云娥,许鸿林,胡建中.水稻条纹叶枯病发生程度的预测模型[J].上海农业学报,1993,9(1):70-74.
132.刘海建,水稻条纹病毒(RSV)免疫检测试剂盒研制与应用[D],扬州:扬州大学.
133.刘力,陈声样,邱并生,康良仪,田波.抗水稻条纹病毒核酶的设计、克隆及体外活性测定[J].中国病毒学,1996,11(2):157-163.
134.刘利华,吴祖建,林奇英,谢联辉.水稻条纹叶枯病细胞病理变化的观察[J].植物病理学报,2000,30(4):306-311.
135.刘卫平.快速ELISA法鉴定马铃薯病毒[J].马铃薯杂志,1997,11(1):11-12.
136.吕英之,邓可京,曲志才,严键,沈大棱.逆转录聚合酶链式反应快速检测水稻条叶枯病毒[J].复旦学报(自然科学版),1999,38(5):591-593..
137.孟清,张鹤龄,宋伯符.应用Dot-ELISA检测PVX、PVY和PVS[J].中国病毒学,1993,8(4):355-371.
138.明艳林,吴祖建,谢联辉.水稻条纹病毒CP、SP进入叶绿素与褪绿症状的关系[J].福建农林大学学报,30(增):147.
139.秦文胜,高东明,陈声祥.灰飞虱体内稻条纹叶枯病毒快速检测技术研究[J].浙江农业学报,1994,6(4):226-/229.
140.邱并生,王晋芳,田波,等.水稻条纹叶枯病毒分子生物学的研究(2)—外壳蛋白基因的合成、克隆和表达[J].科学通报,1991,36(20):1578-1581.
141.邱美强,水稻条纹病毒代表分离物致病性与部分基因分子变异[D],2005,福州:福建农林大学.
142.曲志才,沈大棱,徐亚南,谈家桢,Roger H.水稻条纹叶枯病毒基因产物在水稻和昆虫体内的Western印迹分析[J].遗传学报,1999,26(5):512-517.
143.阮义理,金登迪,许如银.水稻条纹叶枯病发生与流行的研究[J].浙江农业科学,1983,(2):79-81.
144.阮义理,日本水稻条纹叶枯病的研究进展[M]。国外农学(水稻).1986,(5):25-28.
145.田波.植物病毒研究方法[M].北京:科学出版社,1987.
146.王关林,方宏绮.植物基因工程原理与技术[M].科学出版社,1998.
147.王贵珍,周益军,陈正贤,等.水稻条纹病毒单克隆抗体的制备及检测应用[J].植物病理学报2004,34(4):302-306.
148.王贵珍,周益军,周雪平,等.以直接斑点免疫结合测定法检测灰飞虱体内的水稻条纹病毒[J].浙江大学学报(农业与生命科学版),2005,31(1):37-40.
149.王晋芳,邱并生,田波,等.水稻条纹叶枯病毒分子生物学的研究—外壳蛋白基因的序列分析[J].中国病毒学,1992,7(4):463-466.
150.王明霞.应用PCR微量板杂交法检测植物病毒和类病毒[J].植物保护,1996,22(1):34-35a.
151.王明霞.微量板杂交法和斑点杂交法检测菊花矮化类病毒的比较[J].福建农业大学学报,1996,25(1):56-60b.
152.王维新,史成银,高天翔等.鉴定病毒受体的研究方法[J],海洋湖沼通报,2005,(1):54-60.
153.王锡锋,周广和.大麦黄矮病毒介体麦二叉蚜和麦长管蚜体内传毒相关蛋白的确定.科学通报,2003,48(15:1671-1675.
154.王晓红,叶寅,王苏燕,等.水稻条纹叶枯病毒基因组含vRNA2 ORF片段的克隆、序列分析及其在原核中的表达[J].科学通报,1997,42(4):438-441.
155.韦石泉.植物病毒的免疫电子显微镜技术[J].沈阳农业大学学报,1987,18(1):69-75.
156.魏太云,林含新,吴祖建,等.水稻条纹病毒NS2基因遗传多样性分析[J].中国生物化学与分子生物学报,2003b,19(5):600-605.
157.魏太云,林含新,吴祖建,等.水稻条纹病毒RNA4基因间隔区序列分桥棍合侵染及基因组重组证据[J].徽生物学报,2003c,43(5):577-585.
158.魏太云,林含新,吴祖建,等.水稻条纹病毒两个分离物RNA4基因间隔区的序列比较[J].中国病毒学,2000,15(2):156-162.
159.魏太云,林含新,吴祖建.水稻条纹病毒RNA4基因间隔区的分子变异[J].病毒学报,2001,17(2):144-149.
160.魏太云,水稻条纹病毒的基因组结构及其分子群体遗传[D],2003,福州:福建农林大学.
161.魏太云,王辉,林含新,等.我国水稻条纹病毒RNA3片段序列分析—纤细病毒属重配的又一证据[J].生物化学与生物物理学报,2003,35(1):97-103.
162.吴爱忠,赵艳,曲志才,等.水稻条叶枯病毒(RSV)的SP蛋白在介体灰飞虱内的亚细胞定位[J].科学通报,2001,45(14):1183-1186.
163.谢联辉,魏太云,林含新,等.水稻条纹病毒的分子生物学[J].福建农业大学学报,2001,30(3):269-279.
164.谢联辉,周仲驹,林奇英,宋秀高,谢莉妍.水稻条纹叶枯病的研究Ⅲ.病害的病原性质.福建农 学院学报,1991,20(2):144-149.
165.谢晓慧,张云发,林莉,包绍永,刑玉仙.A蛋白酶联免疫吸附法检测水稻条纹叶枯病毒介体昆虫灰飞虱带毒率的研究.西南农业大学学报,1992,5(3):80-/84.
166.徐宜为.免疫检测技术(第二版).北京:科学出版社.1991,174-176.
167.燕义唐,下晋芳,邱并生,等.水稻条纹叶枯病毒外壳蛋白基因在工程水稻植株中的表达[J].植物学报,1992,34(12):899-906.
168.杨家鸾,刘玉彬,林莉,文艳萍,杨炬.灰飞虱生活史及发生规律观察.云南农业科学,(3):19-21.
169.杨金广,云南省水稻条纹病毒的分子变异[D],2005,云南:云南农业大学.
170.杨荣明,刁春友,朱叶芹,等.江苏省水稻条纹叶枯病上升原因及防治对策[J].植保技术与推广,2002,22(3):9-12.
171.于群,魏太云,林含新,等.我国水稻条纹病毒北京双桥(RSV-SQ)分离物RNA4片段序列分析[J].农业生物技术学报,2000,8(3):225-228.
172.张国柱.马铃薯病毒检测技术NCM-ELISA的应用[J].山西农业科学,1991,4:25-26.
173.张鹤龄.酶联免疫吸附测定(ELISA)及其在植物病毒鉴定中的应用[J].马铃薯杂志,1983,4:35-41b.
174.张宏伟,万由衷,曲志才,张晓宁,叶鸣明,沈大棱.利用噬菌体展示筛选结合水稻条叶枯病毒S蛋白的短肽.复旦大学(自然科学版),2001,40(6):695-696.
175.支月娥,何燕红,顾振芳,水稻条纹叶枯病研究[J].上海交通大学报(农业科学版),2001,19(2):148-150.
176.周益军,刘海建,王贵珍,等.灰飞虱携带的水稻条纹病毒免疫检测[J].江苏农业科学,2004(1):50-51.
177.朱光新,郭福寰,肖志敏,李芝芳,周淑琴.应用免疫电镜对几种马铃薯病毒毒源的鉴定研究[J].马铃薯杂志,1992,6(2):67-72.
178.朱美凤,肖庆璞,王法明,陈毓芩.江南稻区新发生的几种稻病[J].植物保护,1964,2(3):100-102.
2.Aranda M,Fraile A,Dopazo J,et al.Contribution of mutation and RNA recombination to the evolution of a plantpathogenic RNA[J].Journal of Molecular Evolution,1997,(1):81-88.
3.Areya C D,Atreya P L,Thornbury D W et al.Site-directed mutation in potyvirus HC-Pro gene affect helper component acticity cirus accumulation and symptom express in infected tobacco plant [J].Virology,1992,191:106-111.
4.Atreya CD,Raccob B,Pirone T P.Apoint mutation in coat protein abolishes aphid transmissibility of Potyvirus[J].Virology,1990,178:161-165.
5.Bandla MD,L.Campbell,D.E.Ulhnan,et al.Interaction of tomato spotted wilt tospovirus.(TSWV)glycoproteins with a thrips midgut protein,a potential cellular receptor for.TSWV[J].Phytopathol.,1998.88:98-104.
6.Barbier P,Takashi M,Nakamura I,et al.Solubilization and promoter analysis of RNA polymerase from rice stripe virus[J].Journal of Virology,1992,66(10):6171-6174.
7.Boonham N,Welsh K,Smith P.Detection of Potatoviurses using microarray technology:towards a genetic method for Plant viral disease diagnosis[J].Virol Methods,2003,108:181-187.
8.Brunt A.A,K.Cuabtree,M.J.Dallwitz et al.Sweet potato sunken vein clostero virus[M].Viruses of plants,CAB International,Cambrige,United Kingdom.1996.
9.Cai L,Ma X,Kang,et al.Detecting rice stripe virus(RSV) in the small brown planthopper with high specificity by RT-PCR[J].Journal of Virological Methods,2003,112:115-120.
10.Chay C A,Gunasinge UB,Dinesh-kumar S P et al.Aphid transmission and systemic plant infection determinants of barley yellow dwarf Luteovirus-PAV are contained in the coat protein readthrough domain and 17 kDa protein respectively[J].Virology.,1996,219:57-65.
11.Chen,C,Ko W.Studies on the time of rice stripe virus infection and field experiments on disease control.Res[J].Bull.Taichung District Agric.Improv.Sta.(Taiwan) 1986,12:51-59.
12.Chomchan P,Li S,Miranda G,et al.Interactions among proteins coded on rice grassy stunt virus genome.[J].Annuals of phytopathological society of Japan,2000,66:164.
13.DharA.K,SinghR.P.Improvement in the sensitivity of PVYN detection by increasing the cDNA Probe size.Journal of virological method,1994,50(1-3):197-210.
14.Dohi K,Mise K,Furusawa I,et al.RNA-dependent RNA polymerase complexs of Brome mosaic virus:analysis of the molecular structure with monoclonal antibodies[J].Journal of General Virology,2002,83:2879-2890.
15.Dorig R E,Marcil A,Chopra A,et al.The human CD46 molecule is a receptor for measles virus (Edmonston)[J].Cell,1993,75(2):295-305.
16.Elliot R.Molecular biology of the Bunyaviridae[J].Journal of General virology,1990,71:501-522.
17.F.奥斯伯,R.E.金斯顿,J.G.塞德曼,等.1998精编分子生物学实验指南[M],科学出版社.
18.Falk B,Tsai J.Biology and molecular biology of viruses in the genus Tenuivirus[J].Annual Review of Phytopathology,1998,36:139-163.
19.Fukamachi S,Inoe H.Period of infection by rice stripe virus during normal season rice culture in Kagoshima Prefecture[C].Proceeding of the Association for Plant Protection of Kyushu,1989,35:3-4.
20.Galon A.Antiguns Y.Rosher A et al.A zucchini yellow mosaic virus coat Protein gene mutation restores aphid transmissibility but has no effect on multiplication[J].Gen Virology,1992,73:2183-2187.
21.Garret A,Kerlan C,Thomas D..Visualization of a luteovirus in the vector aphid's body by two gold immunolabelling techniques:a comparative study.Journal of virological methods,1991,35(1):93-107.
22.Gergerich RC,H.A.Scott.Determinants in the specificity of virus transmission by leaf-feeding beetles[J].Adv.Dis.vector Res,1991.8:1-14.
23.Gildow F E,Gray S M.The aphid salivary gland lamma basal as a selective barrier associated with vector specific transmission of Barley yellow dwarf leteovirus[J].Phytophathology,1993,83:1293-1302.
24.Gingery R,Nault L,Yamashita S.Relationship between maize stripe virus and rice stripe virus[J].Journal of General Virology,1983,64(3):1765-1770.
25.Govier DA,Kassanis B.Evidence that a component other than the virus particles is neel for aphid transmission of Potato VirusY[J].Virology,1974,57:285-286.
26.Granier F.,Durand-Tardif M.,Casse-Delbart F,et al.Mutation in zucchini yellow mosaic virus HC protein associated with loss of aphid transmissibility[J].Gen Virology,1993,74:2737-2742.
27.Gray SM,N Banerjee.Mechanisms of arthropod transmission of plant and animal viruses[J].Microbiology and Molecular Biology Reviews,1999,63(1):128-148.
28.Gray SM.Plant virus proteins involved in natural vector transmission[J].Trends.Microbid.,1996,4:253-294.
29.Guilley H.,WiptS C.,RichardsK et al.Mucleotide sepuence of cucurbit aphid- bione yellow luteovirus[J].Virology,1994,202:1012-1017.
30. Hamamatsu C, Ishihama A. Ambisense RNA genome of rice stripe virus[J]. Uirusu, 1994, 44(1):19-25.
31. Hamamatsu C, Toriyama S, Toyoda T, et al. Ambisense coding strategy of the rice stripe virus genome: in vitro translation studies[J].J. Gen. Virol, 1993; 74: 1125-1131.
32. Hataya I ,Inoue A.K., Shikata E. PCR-microplate hybridization, method for plant virus detection.Journal of virologicalMehtods, 199A, 46 (2) :223-236.
33. Hayakawa T, Zhu Y, Itoh K, et al. Genetically engineered rice resistant to rice stripe virus, an insect-transmitted virus[J]. Proceeding of the National Academy of Science. USA, 1992, 89:9865-9869.
34. Hayano Y, Kakutani T, Hayashi T, et al. Coding strategy of rice stripe virus: major nonstructural protein is encoded in viral RNA segment 4 and coat protein in RNA complementary to segment 3 [J]. Virology. 1990, 177: 372-374.
35. Hayano-Saito Y, Tsuji T, Fujii K, et al. Localization of the rice stripe disease resistance gene, Stvb-I : by graphical genotyping and linkage analyses with molecular markers [J]. Theoretical and Applied Genetics, 1998,96(8): 1044-1049.
36. Hayashi T, Usugi T, Nakano M, et al. On the strains of rice stripe virus(1): an attempt to detect strains by difference of molecular size of disease-specific proteins[J]. Proceeding of the Association for Plant Protection of Kyushu, 1989,35(1): 1-2.
37. Hibino H. Biology and Epidemiology of rice viruses[J]. Annual Review of Phytopathology, 1996, 34: 249-174.
38. Holland J, Spindler K, Horodyshi F. Rapid evolution of RNA genomes[J]. Science, 1982, 215: 1577-1585.
39. Hong S S, Karayan L,Tournier J,et al.A denovirus type 5 fiber konb bingds to MHC class 1α2 domain at the surface of human epithelial and B lymphoblastoid cells [J]. EMBO, 1997,16(9):2294-2306.
40. Ishihama H. Nucleotide sequence and rice stripe virus RNA segment 2[J]. Journal of General Virology, 1993, 74(4): 769-733.
41. Ishii M, Ono K. On strains of rice stripe virus[J]. Annuals of the phytopathological society of Japan,1967, 32(1): 83.
42. Ito K, Okada M. Bionomics of the small brown planthopper in relation to the transmission of rice stripe virus[J]. Plant Protection (Japan), 1985, 39(11): 525-53.
43. Kakutani T, Hayano Y, Hayashi T, et al. Ambisense segment 3 of rice stripe virus: the first instance of a virus containing two ambisense segments[J]. Journal of General Virology, 1991, 72: 465-468.
44. Kakutani T, Hayashi T, Minobe Y. Ambisense segment 3 of rice stripe virus: the first instance of a virus containing two ambisense segments[J].Journal of General,1991,72(2):465-4.68.
45.Kisimoto R,Yamada Y.Present Status of Contolling Rice Stripe Virus[C].Edited by Hadidi A,Khetarpal R and Oganezawa H.Chapter 35:470-483.
46.Kisimoto R,Yamada Y.Present Status of Controlling Rice Stripe Virus[A].In:Hadidi A,Khetarpal RK,Koganezawa H(ed).Plant Virus Disease Control[C].St.Paul.Minnesota:APS Press,1991:470-481.
47.Kisimoto R.Ecology office stripe disease[J].Hereditas(In Japan),1972,(12):34-40.
48.Kisimoto R.Genetic variation in the ability of a planthopper vector,Laodelphaxstriatelllus Fallen to acquire the rice stfie virus[J].Virology,1967,32(1):144-152.
49.Kiso H,Yamamoto T.Infection and symptom development in rice stripe diseases,with special reference to disease specific protein other than virus.Rev[J].Plant Protect.Res.(Japan) 1973,6:75-100.
50.Lee G P,Min BE,Kim CS.Plant virus cDNA chip hybridization for detection and dieffrentiation of four cucurbit-infecting Tobamoviruss[J].Virol Methods,2003,110:19-24.
51.Li W H,Moore M J,Vasilieva N,et al.Angiotensin-converting enzyme 2 is a functional receptor for SARS coronavims[J].Nature,2003,426(6965):450-454.
52.Liang D,Ma X,Qu Z.PVC2 of rice stripe tenuivirus is a component of fibrillar electron-opaque inclusion body.Abstract of 19~(th) American Society for Virology[M],Colorado,USA,2000,7:15-19.
53.Lin Hanxin,Wei Taiyun,Wu Zujian,et al.Molecular variability in coat protein and disease-specific protein genes among isolates of rice stripe virus in China[M].Abstract for the ⅪInternational Congress of Virology,Australia,Sydney,1999:235-236.
54.ltamirez B,Haenni A.Molecular biology of tenuivinrses:a remarkable group of plant vinise[J].Jouranl of General Yrology,1994,75:467-475.
55.Ludwig G V,Israel B A,Christensen B M,et al.Role of La crosse virus glycoproteins in attachment of virus to host cell[J].Virology,1991,181:564-571.
56.Marjolein Kikkert,Cor Meurs,Fennet van de Wetering,Simone Dorfm(u|¨)ller,Dick Peters,Richard Kormelink,and Rob Goldbach.Binding of tomato spotted wilt vires to a 94-kDa thrips protein.Phytopathology,1998,88:63-69.
57.Medeiros RB,D E Ullman,J.L.Sherwood,et al.Immunoprecipitation of a 50 kDa protein:a candidate receptor component for tomato spotted wilt tospovirus in its thrips vector[J].Virus Research,2000,67(2):109-118
58.Morris P.C.著,朱传楹译.生物防治与植物保护.国外农学-植物保护,1992,5(4):44-46.
59.Nishiguchi M,Mori M,Suzuki F.Specific detection of a severe strain of sweet potato feathery mottle virus(SPFMV-S) by reverse transcription and polymerase chain reaction(RT-PCR)[J]. Annuals of the phytopathological society of Japan, 1995, 61:119-122.
60. Nolasco G, de Bias C, Torres V, Ponz F. A method combining immunocapture and PCR amplification in a microtiter plate for the detection of plant virus and subviral pathogens. Journal of virological Methods, 1993.,45(2):201-218.
61. Noris E,A.MVaira,P.Caciagli,V. Masenga,et al. Amino acids in the capsid protein of tomato yellow leaf curl virus that are crucial for systemic, infection, particle formation, and insect transmission [J].Virology, 1998,72(12): 10050-10057.
62. Nuss DL, D. Summers. Variant dsRNAs associated with transmission-defective isolates of wound tumor virus represent terminally conserved remnants of genome segments [J]. Virology, 1984, 133(2): 276-288.
63. Okuyama S, Asuyama H. The propagation of the rice stripe virus in the body of the vector[C], in: Programs and Abstracts of the Symposium on Vectors of Plant Viruses, 1962, 288: 10.
64. Omura T, Hibino H, Usugi T. Detection of rice viruses in plants and individual insect vectors by Latex Flocculation Test[J]. Plant Diseases , 1984, 68: 374-378.
65. Omura T, J, Yan, B. Zhong, et al. The P2 protein of rice dwarf phytoreovirus is required for adsorption of the virus to cells of the insect vector [J]. Virology, 1998, 72(11): 9370-9373.
66. Omura T, Takahashi Y, Shohara K, et al. Production of monoclonal antibodies against rice stripe virus for the detection of virus antigen in infected plants and viruliferous insects[J]. Annals of the phytopathological society of Japan, 1986a, 52(2): 270-277.
67. Omura T, W. Maruyama, et al. Involvement in virus infection to insect vector cells of the P2 outer capsid proteins of rice gall dwarf and rice dwarf phytoreoviruses [J]. Phytopathol, 1997, 87 : S72.
68. Peiffer H L., Glidow F E., Gray S M. Two distinct mechanisms regulate luteovirus transmission efficiency and specificity at the aphid salivary gland. [J] Gen Virology, 1997,78: 495-503.
69. Pirone T P. Viral genes and gene products that determine insect transmissibility [J]. Seminar in Virology, 1991,2:81-87.
70. Pirone T P., Blanc S. Helper-dependent transmission of plant viruses [J]. Annu Rev Phytopathol, 1996, 34: 227-247.
71. Qiu W, Geske S, Hichey C. Tomato spotted reassortrnent and genome segment-specific adaptation[J]. Urology, wilt, 1998, 244: 186-194.
72. Rajcani J. Molecular mechanisms of virus spread and virion components as tools of virulence [J].Acta Microbiol Immunol Hung, 2003, 50(4): 407-443.
73. Ramirez B, Haenni A. Molecular biology of tenuiviruses, a remarkable group of plant viruses[J].Journal of General Virology, 1994, 75: 467-475.
74. Ramirez B, Lozano I, Constantino L, et al. Complete nucleotide sequence and coding strategy of rice hoja blanca virus RNA4[J].Journal of General Virology,1993,74:2463-2468.
75.Resende R.,de Haan P,de Arila AC,et al.Defective isolates of tomato spotted wilt virus.Virus-Thrips-Plant Interactions of Tomato Spotted Wilt Virus[J].Virology,1991.72:2375-2383.
76.Robinson D.J.,Romero J..Sensitivity and specificity of nucleic acid probes for potato leafrollluteovirus detection.Journal of virological Methods,1991,34(2):209-219.
77.Ryu C J,Cho D Y,Gripon P,et al.An 80-kilodalton protein that binds to the Pre-Sldomain of hepatitis B virus[J].Virology,2000,74(1):110-116.
78.Salas-Benito J S,Del-Angel R M.Identification of two surface proteins from C6/36 cells that bind Dengue type 4 virus[J].Virology,1997,71(10):7246-7252.
79.Shinkai A.Studies on insect transmission of rice virus diseases in Japan[J].Bull.Natl.Instit.Agric.Sci.Series C 1962,14:104-112.
80.Singh R.R,Kutz J,Boiteau G.Detection of stylet-borne and circulative potato viruses in aphids by duplex reverse transcription polymerase chain reaction.Journal of virological Methods,1996,59(1-2):189-196.
81.Takahashi M,Toriyama S,Kikuchi Y,Hayakawa T,Ishihama A.Complementarity between the 5'-and 3'-terminal sequences of rice stripe virus RNAs[J].Journal of General virology,1990,71:2817-2821.
82.Takahashi M,Toriyama S,Hamamatsu C,Ishihama A.Nucleotide sequence and possible ambisense coding strategy of rice stripe virus RNA segment 2.Journal of General Virology,1993,74(4):769-763.
83.Takahashi M.Expression of rice stripe virus 22.8k protein in insect cells[J].Annuals of the phytopathlogical society of Japan,1998,65(3):342.
84.Thomas C.Mettenleiter Brief overview on cellular virus receptors[J].Virus Research,2002,82:3-8.
85.Thornhbury T P.Comparative sequence of helper component(HC) region of PVY and HC-defective strain potato virus C[J].virology,1990,178:573-578.
86.Tomaro,M.W.Maruyama,A.Kiknche.Protein P2 results in nontransmissibility by the insect vector of rice dwarf phytoreovims[J].Virology,1997,71:8019-8023.
87.Toriyama S,Takahashi M,Sano Y,Shimizu T,Ishihama A..Nucleotide sequence of RNA1 the largest genomic segment of rice stripe virus,the prototype of the tenuiviruses[J].Journal of General Virology,1994,75:3569-3579.
88.Toriyama S.Characterization of rice stripe virus:a heavy component carrying infectivity[J].Journal of General Virology,1982,61:187-195.
89.Toriyama S.Rice stripe virus[J].CMI/AAB Descriptions of Plant Virus,1983,No:269.
90.Toriyama S.Rice stripe virus:prototype of a new group of viruses that replicated in plants and insects[J].Microbllogical Sciences,1986,3:347-351.
91.Toriyama S.Rice stripe virus[C].CMI/ABB.Desccription of plant viruses.1983,269:15.
92.Walsh K,North J,Barker I.,Boonham N.Detection of different strains of Potato virus Y and their mixed infections using competitive fluorescent RT-PCR.Journal of virological Methods,2001,91(2):167-173.
93.Wang J Y.,Chay F E.,Gray S M.Readthrough protein associated with cirions of barley yellow dwarf leuteovirus and its potential role in regulation the efficiency of aphid transmission[J].Virology,1995,206:954-962.
94.Welnicki M,Zekanowski C,Zagorski W.Digoxigenin-labelled molecular probe for the simultaneous detection of three potato pathogens:potato spindle tuber viroid(PSTVd),potato virus Y(PVY),and potato leaf roll virus(P LRV).Acta Biochim Pol,1994,41(4):473-475.
95.White M A.The yeast two-hybrid system:forward and reverse[J].Proc Natl Acad Sci USA,1996,93(19):10001-10003.
96.Wijkamp I.,van Lent J.,Kormelink R.,et al.Multiplication of tomato spotted wolt virus in its insect vector,Frankliniella occidentals[J].Gen Virology,1993,74:341-349.
97.Yamada W,Shiomi T,Yamamoto H.Studies on the stripe disease of rice plant,Ⅱ.On the disease occurrence in Okayama Prefecture and its control[J].Okayama Pref.Agric.1955,52:113-124.
98.Yah J.M.Tomaru,A.Takahachi,et al.P2 protein encoded by genome segment S2 of rice dwarf phytoreovirus is essential for vires infection[J].Virology,1996,224:539-541.
99.Yasuo S,Ishii M,Yamaguchi T.Studies on rice stripe disease Ⅰ.Epidemiological and ecological studies on rice stripe disease in the Kanto-Tosan district of central part of Japan[M].Review of Plant Protection Research,1968,1:96-104.
100.Zhang H,Wu R.Effcient regenerationof transgenic plant from rice protoplasts and correctly regulated expression of the foreign gene in the plant[J].Theor Appl Genet,1988,76:835-840.
101.Zhu Y,Hayajawa T,Toriyama S.Compete nucleotide sequence of RNA4 of rice stripe virus isolate T and comparison with anther isolate and with maize stripe virus[J].Journal of General lrrology,1992,73:1309-1312.
102.Zhu Y,Hayakawa T,Toriyama S,Takahashi M.Complete nucleotide sequence of RNA3 of rice stripe virus:an ambisense coding strategy[J].Journal of General Virology,1991,72:763-767.
103.白艳菊,李学湛,吕典秋.应用DAS-ELISA法同时检测马铃薯病毒[J].中国马铃薯,2000,4(3):143-44
104.陈爱香.水稻条纹病毒NS2基因的分子变异及其原核表达[D],2004,福州:福建农林大学.
105.陈光育,龚林根,秦维勒,傅华欣.酶联法在水稻条纹叶枯病流行预测上的应用[J].植物保护学 报,1991,18(1):49-52.
106.陈光育.酶联免疫吸附试验检测水稻条纹叶枯病介体昆虫带毒率[J].植物保护学报,1984,11(2):73-78.
107.陈京,胡伟贞,张成良,宋淑敏,王树琴.番茄环斑病毒单克隆抗体细胞株的建立及检测方法的研究[J].植物检疫,1994,8(1):1.
108.陈庆忠,柯文华.水稻条纹叶枯病主要感染时期之推定及适期防治试验[J].台中区农业改良场研究简报,1986,12:51-60.
109.陈庆忠,柯文华.台湾水稻条纹叶枯病流行学研究[J].植物保护会刊,1989,31(3):290-303.
110.陈声祥.水稻病毒发生现状及研究进展[J].浙江农业科学,1996,(1):41-42.
111.程兆榜,杨荣明,周益军,等.江苏稻区水稻条纹叶枯病发生新规律[J].江苏农业科学,2002(1):39-41
112.丁天兵,任君萍,马文煜.毒受体的研究方法[J].中国病毒学,2006,21(2):198-193
113.高东明,秦文胜.抗性品种对预防水稻条纹叶枯病发生和流行的研究[J].浙江农业学报,1994,6(3):141-149.
114.高苓昌,宋克勤,张洪瑞,等.黄淮稻区水稻条纹叶枯病的发病特点与综合防治[J].山东农业科学,2006(3):66-67.
115.弓利英,夏立,马丽,等.沿黄稻区水稻条纹叶枯病的发生及防治技术[J].河南农业科学,2006(7):64-65.
116.贺鹏飞.PLRV的IC-RT-PCR和DAS-ELlsA两种检测方法的比较[D].湖南农业大学学士学位论文,1999,12-13.
117.贾慧.基因芯片技术在百合病毒检测中的应用[D].河北农业大学硕士学位论文,2004.
118.蒋耀培,李建刚.上海地区水稻条纹叶枯病的发生与防治技术初探[J].上海农业科技,2005(5):37-38.
119.鞠振林,朱笑梅,薛爱红.改进的直接组织斑点免疫测定法在植物病毒及细菌检测中的应用[J].植物病理学报,1993,23(4):366-371.
120.李汝刚,郭军,鞠振林,朱笑梅,梁华,王远程.马铃薯x、Y病毒的单克隆抗体及其在马铃薯病毒检测上的应用[J].马铃薯杂志,1992,6(4):233-236.
121.李毅,陈章良.植物病毒胞间运动的分子生物学和细胞学研究进展[J].植物生理学通讯,1995,31(6):463-469.
122.林付根,成长庚,赵阳.水稻条纹叶枯病治虫防病策略的实践体会[J].中国植保导刊,2006(1):13-15.
123.林含新,林奇英,谢联辉.水稻条纹病毒分子生物学研究进展[J].中国病毒学报,1997,12(3):203-209.
124.林含新,魏太云,吴祖建,等.我国水稻条纹病毒7个分离物的致病性和和化学特性比[J].福建农 业大学学报,2002,31(2):164-167.
125.林含新,魏太云,吴祖建,等.我国水稻条纹病毒一个强致病性分离物的RNA4序列测定与分析[J].微生物学报,2001,41(1):25-30.
126.林含新,魏太云,吴祖建,等.应用PCR-SSCP技术快速检测我国水稻条纹叶枯病毒的分子变异[J].中国病毒学,2001,16(2):166-169.
127.林含新,吴祖建,林奇英,等.水稻品种对水稻条纹病毒的抗性鉴定及其作用机制研究初报[M].植物病毒与病毒病防治(刘仪主编),中国农业科技出版社,北京,1997:p188-192.
128.林均安,高锦梁,洪健主编.实用生物电子显微技术[M].沈阳:辽宁科学技术出版社.1988.
129.林奇田,林含新,吴祖建,林奇英,谢联辉.水稻条纹病毒外壳蛋白和病害特异性蛋白在寄主体内的积累[J].福建农业大学学报,1998,27(3):322-326.
130.林奇英,谢联辉,周仲驹,谢莉妍,吴祖建.水稻条纹叶枯病的研究Ⅰ.病害的分布和损失[J].福建农学院学报,1990,19(4):421-425.
131.刘德钧,韩长安,黄拔山,王金其,陈建生,朱云娥,许鸿林,胡建中.水稻条纹叶枯病发生程度的预测模型[J].上海农业学报,1993,9(1):70-74.
132.刘海建,水稻条纹病毒(RSV)免疫检测试剂盒研制与应用[D],扬州:扬州大学.
133.刘力,陈声样,邱并生,康良仪,田波.抗水稻条纹病毒核酶的设计、克隆及体外活性测定[J].中国病毒学,1996,11(2):157-163.
134.刘利华,吴祖建,林奇英,谢联辉.水稻条纹叶枯病细胞病理变化的观察[J].植物病理学报,2000,30(4):306-311.
135.刘卫平.快速ELISA法鉴定马铃薯病毒[J].马铃薯杂志,1997,11(1):11-12.
136.吕英之,邓可京,曲志才,严键,沈大棱.逆转录聚合酶链式反应快速检测水稻条叶枯病毒[J].复旦学报(自然科学版),1999,38(5):591-593..
137.孟清,张鹤龄,宋伯符.应用Dot-ELISA检测PVX、PVY和PVS[J].中国病毒学,1993,8(4):355-371.
138.明艳林,吴祖建,谢联辉.水稻条纹病毒CP、SP进入叶绿素与褪绿症状的关系[J].福建农林大学学报,30(增):147.
139.秦文胜,高东明,陈声祥.灰飞虱体内稻条纹叶枯病毒快速检测技术研究[J].浙江农业学报,1994,6(4):226-/229.
140.邱并生,王晋芳,田波,等.水稻条纹叶枯病毒分子生物学的研究(2)—外壳蛋白基因的合成、克隆和表达[J].科学通报,1991,36(20):1578-1581.
141.邱美强,水稻条纹病毒代表分离物致病性与部分基因分子变异[D],2005,福州:福建农林大学.
142.曲志才,沈大棱,徐亚南,谈家桢,Roger H.水稻条纹叶枯病毒基因产物在水稻和昆虫体内的Western印迹分析[J].遗传学报,1999,26(5):512-517.
143.阮义理,金登迪,许如银.水稻条纹叶枯病发生与流行的研究[J].浙江农业科学,1983,(2):79-81.
144.阮义理,日本水稻条纹叶枯病的研究进展[M]。国外农学(水稻).1986,(5):25-28.
145.田波.植物病毒研究方法[M].北京:科学出版社,1987.
146.王关林,方宏绮.植物基因工程原理与技术[M].科学出版社,1998.
147.王贵珍,周益军,陈正贤,等.水稻条纹病毒单克隆抗体的制备及检测应用[J].植物病理学报2004,34(4):302-306.
148.王贵珍,周益军,周雪平,等.以直接斑点免疫结合测定法检测灰飞虱体内的水稻条纹病毒[J].浙江大学学报(农业与生命科学版),2005,31(1):37-40.
149.王晋芳,邱并生,田波,等.水稻条纹叶枯病毒分子生物学的研究—外壳蛋白基因的序列分析[J].中国病毒学,1992,7(4):463-466.
150.王明霞.应用PCR微量板杂交法检测植物病毒和类病毒[J].植物保护,1996,22(1):34-35a.
151.王明霞.微量板杂交法和斑点杂交法检测菊花矮化类病毒的比较[J].福建农业大学学报,1996,25(1):56-60b.
152.王维新,史成银,高天翔等.鉴定病毒受体的研究方法[J],海洋湖沼通报,2005,(1):54-60.
153.王锡锋,周广和.大麦黄矮病毒介体麦二叉蚜和麦长管蚜体内传毒相关蛋白的确定.科学通报,2003,48(15:1671-1675.
154.王晓红,叶寅,王苏燕,等.水稻条纹叶枯病毒基因组含vRNA2 ORF片段的克隆、序列分析及其在原核中的表达[J].科学通报,1997,42(4):438-441.
155.韦石泉.植物病毒的免疫电子显微镜技术[J].沈阳农业大学学报,1987,18(1):69-75.
156.魏太云,林含新,吴祖建,等.水稻条纹病毒NS2基因遗传多样性分析[J].中国生物化学与分子生物学报,2003b,19(5):600-605.
157.魏太云,林含新,吴祖建,等.水稻条纹病毒RNA4基因间隔区序列分桥棍合侵染及基因组重组证据[J].徽生物学报,2003c,43(5):577-585.
158.魏太云,林含新,吴祖建,等.水稻条纹病毒两个分离物RNA4基因间隔区的序列比较[J].中国病毒学,2000,15(2):156-162.
159.魏太云,林含新,吴祖建.水稻条纹病毒RNA4基因间隔区的分子变异[J].病毒学报,2001,17(2):144-149.
160.魏太云,水稻条纹病毒的基因组结构及其分子群体遗传[D],2003,福州:福建农林大学.
161.魏太云,王辉,林含新,等.我国水稻条纹病毒RNA3片段序列分析—纤细病毒属重配的又一证据[J].生物化学与生物物理学报,2003,35(1):97-103.
162.吴爱忠,赵艳,曲志才,等.水稻条叶枯病毒(RSV)的SP蛋白在介体灰飞虱内的亚细胞定位[J].科学通报,2001,45(14):1183-1186.
163.谢联辉,魏太云,林含新,等.水稻条纹病毒的分子生物学[J].福建农业大学学报,2001,30(3):269-279.
164.谢联辉,周仲驹,林奇英,宋秀高,谢莉妍.水稻条纹叶枯病的研究Ⅲ.病害的病原性质.福建农 学院学报,1991,20(2):144-149.
165.谢晓慧,张云发,林莉,包绍永,刑玉仙.A蛋白酶联免疫吸附法检测水稻条纹叶枯病毒介体昆虫灰飞虱带毒率的研究.西南农业大学学报,1992,5(3):80-/84.
166.徐宜为.免疫检测技术(第二版).北京:科学出版社.1991,174-176.
167.燕义唐,下晋芳,邱并生,等.水稻条纹叶枯病毒外壳蛋白基因在工程水稻植株中的表达[J].植物学报,1992,34(12):899-906.
168.杨家鸾,刘玉彬,林莉,文艳萍,杨炬.灰飞虱生活史及发生规律观察.云南农业科学,(3):19-21.
169.杨金广,云南省水稻条纹病毒的分子变异[D],2005,云南:云南农业大学.
170.杨荣明,刁春友,朱叶芹,等.江苏省水稻条纹叶枯病上升原因及防治对策[J].植保技术与推广,2002,22(3):9-12.
171.于群,魏太云,林含新,等.我国水稻条纹病毒北京双桥(RSV-SQ)分离物RNA4片段序列分析[J].农业生物技术学报,2000,8(3):225-228.
172.张国柱.马铃薯病毒检测技术NCM-ELISA的应用[J].山西农业科学,1991,4:25-26.
173.张鹤龄.酶联免疫吸附测定(ELISA)及其在植物病毒鉴定中的应用[J].马铃薯杂志,1983,4:35-41b.
174.张宏伟,万由衷,曲志才,张晓宁,叶鸣明,沈大棱.利用噬菌体展示筛选结合水稻条叶枯病毒S蛋白的短肽.复旦大学(自然科学版),2001,40(6):695-696.
175.支月娥,何燕红,顾振芳,水稻条纹叶枯病研究[J].上海交通大学报(农业科学版),2001,19(2):148-150.
176.周益军,刘海建,王贵珍,等.灰飞虱携带的水稻条纹病毒免疫检测[J].江苏农业科学,2004(1):50-51.
177.朱光新,郭福寰,肖志敏,李芝芳,周淑琴.应用免疫电镜对几种马铃薯病毒毒源的鉴定研究[J].马铃薯杂志,1992,6(2):67-72.
178.朱美凤,肖庆璞,王法明,陈毓芩.江南稻区新发生的几种稻病[J].植物保护,1964,2(3):100-102.
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