新疆野生石竹种质资源收集、保存、评价及利用研究
摘要
本研究对新疆野生石竹种质资源进行了全面调查和收集,并从形态学、植物细胞学、孢粉学及分子生物学4个层面,系统地研究了新疆野生石竹当前的资源状况。主要结论如下:
1.对位于新疆中部的天山山脉和北部的阿尔泰山脉2个野生石竹自然分布区进行实地调查。共采集野生石竹标本160份和26个群体528个株系的种子。并对其形态特征进行了系统调查和分析。
2.在对新疆野生石竹进行实地调查和对采集标本分析鉴定以及对主要标本馆进行现存标本查阅的基础上。编制了新疆野生石竹分种检索表。对发现的两个新类群进行了命名。
3.以采自新疆天山山脉和阿尔泰山脉的13个种和1个变种的野生石竹花粉、种子和叶片为材料,利用扫描电镜对其形态进行系统观察和比较,并通过研究结果对新疆野生石竹的分类、演化和亲缘关系进行了探讨。
4.采用PCR产物直接测序方法对新疆野生石竹的10个种进行了ITS区序列测定,并对新疆野生石竹种间的亲缘关系进行了分析。
Species resource of Xinjiang wild Dianthus was collected and comprehensively investigated in this study。
1. Based on field investigation of 2 wild Dianthus natural distribution areas: Tianshan Mountains located in the middle of Xinjiang and Altay Mountains which was the north of Xinjiang.
2. We based on a field investigation and analysis of the collected Xinjiang Wild Dianthus specimens and museum specimens, and revised the position of Xinjiang Wild Dianthus in classification systems. Two new species were discovered and named Dianthus altaicus L.X.Dong & C.Y.Yang and Dianthus nanshanicus C.Y.Yang & L.X.Dong and at last make the list of Dianthus species family.
3. We collected pollen.seed and leaf of 10 wild Dianthus species in the Tianshan Mountains and Altay Mountains from Xinjiang,which were observed under the scanning electron microscope (SEM).
4. We measured the ITS sequences using Direct sequence of PCR products methods, and analyzed the relationship among each group of Xinjiang Wild Dianthus. The results showed that the length ITS sequences in 10 species of Xinjiang wild Dianthus were from 610bp to 614bp), had smaller variation and the difference is 4bp.Compared with the length of the ITS of L.coronata in out-group genus Lychnis which had obvious differences.
1.对位于新疆中部的天山山脉和北部的阿尔泰山脉2个野生石竹自然分布区进行实地调查。共采集野生石竹标本160份和26个群体528个株系的种子。并对其形态特征进行了系统调查和分析。
2.在对新疆野生石竹进行实地调查和对采集标本分析鉴定以及对主要标本馆进行现存标本查阅的基础上。编制了新疆野生石竹分种检索表。对发现的两个新类群进行了命名。
3.以采自新疆天山山脉和阿尔泰山脉的13个种和1个变种的野生石竹花粉、种子和叶片为材料,利用扫描电镜对其形态进行系统观察和比较,并通过研究结果对新疆野生石竹的分类、演化和亲缘关系进行了探讨。
4.采用PCR产物直接测序方法对新疆野生石竹的10个种进行了ITS区序列测定,并对新疆野生石竹种间的亲缘关系进行了分析。
Species resource of Xinjiang wild Dianthus was collected and comprehensively investigated in this study。
1. Based on field investigation of 2 wild Dianthus natural distribution areas: Tianshan Mountains located in the middle of Xinjiang and Altay Mountains which was the north of Xinjiang.
2. We based on a field investigation and analysis of the collected Xinjiang Wild Dianthus specimens and museum specimens, and revised the position of Xinjiang Wild Dianthus in classification systems. Two new species were discovered and named Dianthus altaicus L.X.Dong & C.Y.Yang and Dianthus nanshanicus C.Y.Yang & L.X.Dong and at last make the list of Dianthus species family.
3. We collected pollen.seed and leaf of 10 wild Dianthus species in the Tianshan Mountains and Altay Mountains from Xinjiang,which were observed under the scanning electron microscope (SEM).
4. We measured the ITS sequences using Direct sequence of PCR products methods, and analyzed the relationship among each group of Xinjiang Wild Dianthus. The results showed that the length ITS sequences in 10 species of Xinjiang wild Dianthus were from 610bp to 614bp), had smaller variation and the difference is 4bp.Compared with the length of the ITS of L.coronata in out-group genus Lychnis which had obvious differences.
引文
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