抑制素主动免疫对青年奶山羊生殖的影响
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摘要
为了建立西农萨能奶山羊抑制素主动免疫的方法,探讨对其体内主要生殖激素和产羔率的影响,提高青年奶山羊的繁殖力,选用饲养于西北农林科技大学萨能羊原种场年龄1.5岁、体重接近的8只青年奶山羊,随机分为两组,每组4只,在发情季节利用诱导同期发情、抑制素主动免疫方法、琼脂双向扩散和激素放射免疫测定技术研究抑制素主动免疫后,对抗体效价、生殖激素浓度和产羔数的影响。试验组用人抑制素α亚单位片段与牛血清白蛋白耦合后,颈部多点皮下注射进行免疫,间隔4w进行3次免疫,对照组用生理盐水。第二次加强免疫结束后,对8只青年奶山羊颈部肌肉注射氯前列烯醇(PG)0.1mg/只,间隔11d,共注射3次。第三次注射PG后24h,以6h间隔牵引公羊试情,观察并记录8只羊首次接受的爬跨时间。第三次注射PG后,间隔6h采血,8只羊发情后12h均用牵引交配的方式进行第一次配种,间隔12h再补配一次,并做记录。试验结果显示:试验组4只羊第1次免疫后均产生了抑制素抗体,抗体滴度随免疫次数的增加而增加,末次免疫后试验组均获得了较高的抑制素抗体效价。第三次注射PG后试验组外周血促卵泡素(FSH)平均水平为11.68±0.13 ng/mL,对照组为5.99±0.13 ng/mL,两者差异显著(P < 0.05);试验组FSH水平在注射PG后42h达到峰值,对照组48h达到峰值,前者峰值的出现早于后者;试验组中FSH的峰值水平显著高于对照组(P < 0.05)。试验组促黄体素(LH)平均水平为4.29±0.26 mIU/mL,对照组为5.30±0.26 mIU/mL,两者差异不显著(P > 0.05),试验组LH水平注射PG后42h达到峰值,对照组在注射PG后48h达到峰值,前者峰值的出现早于后者峰值。孕酮(P)水平在注射PG后持续下降。试验组孕酮平均水平为2.77±0.74 ng/mL,对照组为2.06±0.74 ng/mL,差异不显著(P > 0.05)。第三次注射PG后0h和6h两个时刻,试验组孕酮水平显著高于对照组(P < 0.05),6h后两组孕酮水平差异不显著。第三次注射PG后雌二醇(E2)的水平先升高后降低,试验组E2平均水平为19.37±0.68 pg/mL,对照组为11.83±0.56 pg/mL,差异显著(P < 0.05)。第三次PG注射后第42h和48h,E2的水平显著高于对照组(P < 0.05),试验组E2峰值出现时刻比对照组晚6h。两组试验动物受胎率均为100%,试验组产羔9只,产羔率为180%,对照组产羔5只,产羔率为125%。试验组产羔数量多于对照组,而且产双羔的羊只也多于对照组,经卡平方检验,试验组产羔率显著高于对照组(P < 0.05)。结论:抑制素主动免疫后,青年奶山羊均能产生抗体,第二次加强免疫后抗体水平最高;抑制素主动免疫对青年奶山羊发情期间FSH、E2的平均水平有显著提高,对LH、P的没有显著改变,试验组FSH、LH峰值出现均早于对照组;抑制素主动免疫对青年奶山羊诱导其提前发情,对产羔数有提高的作用。
The inhibin active immunization method, agar diffusion technique and radioimmunoassay are used to predict the antibody titers and hormonal level on youth Xinong Saanen dairy goats in this experiment. The main content is as the following:
Eight 1.5 years old and weigh close youth dairy goats are allocated to two groups raised in the NWSUAF Saanen Goat Growing Field. The immunized group (n=4) are treated with s.c. injections of human inhibin vaccine into four sites followed by two booster injection at 4-week intervals and the control group (n=4) are treated with an equal volume saline. After the two booster injection, estrous cycles are synchronized by i.m. injection of 0.1mg PG. Two further PG injections are given at 11-day intervals. The estrus time is recorded after each PG injection. All estrus goats are bred in natural mating method after the third PG injection.The results show that: all in immunized group produce antibodies, and the antibody titres rise with increased of the immunized time. There are high titres in immunized group after the second booster injection, reaching 1:64, 1:128, 1:64 and 1:64 respectively. FSH mean concentrations in 72 hour after the third PG injection are significantly higher (P< 0.05) in inhibin-immunized goats than in the controls. The immunized group FSH surge level come at 48 hour after PG injection, while the control group at 56 hour. The surge value in FSH is higher significantly (P < 0.05) than the control group. Plasma concentrations of LH did not differ significantly between inhibin-immunized and control goats (P > 0.05). In both groups, PG injection is followed by a preovulatory sure in LH coincident with the FSH surge. Plasma concentrations of P decrease after the third PG injection, and the P mean concentrations in 6 hour after PG injection are significantly higher(P< 0.05)in inhibin-immunized goats than in the controls, but the overall mean concentrations after PG injection are not significantly between the two group. Plasma concentrations of E2 rise after PG-induced luteolysis to reach a peak value which is significantly (P< 0.05) higher in immunized goats than in controls. The concentrations then fall and remain relatively low. The time of peak value come later in immunized group than the control group. The conception rate is 100% in both groups. Immunized-goats produce 9 kids while only 5 in the controls. A 55% increase in kidding rate is recorded for youth goats in immunized group. The kidding rate is higher significantly between the two groups.The results demonstrate that the active immunization against inhibin enhances the secretion of FSH, E2 and P on youth dairy goat in estrous period. Immunization against inhibin induces the estrous and enhances the kidding of youth dairy goat.
The inhibin active immunization method, agar diffusion technique and radioimmunoassay are used to predict the antibody titers and hormonal level on youth Xinong Saanen dairy goats in this experiment. The main content is as the following:
Eight 1.5 years old and weigh close youth dairy goats are allocated to two groups raised in the NWSUAF Saanen Goat Growing Field. The immunized group (n=4) are treated with s.c. injections of human inhibin vaccine into four sites followed by two booster injection at 4-week intervals and the control group (n=4) are treated with an equal volume saline. After the two booster injection, estrous cycles are synchronized by i.m. injection of 0.1mg PG. Two further PG injections are given at 11-day intervals. The estrus time is recorded after each PG injection. All estrus goats are bred in natural mating method after the third PG injection.The results show that: all in immunized group produce antibodies, and the antibody titres rise with increased of the immunized time. There are high titres in immunized group after the second booster injection, reaching 1:64, 1:128, 1:64 and 1:64 respectively. FSH mean concentrations in 72 hour after the third PG injection are significantly higher (P< 0.05) in inhibin-immunized goats than in the controls. The immunized group FSH surge level come at 48 hour after PG injection, while the control group at 56 hour. The surge value in FSH is higher significantly (P < 0.05) than the control group. Plasma concentrations of LH did not differ significantly between inhibin-immunized and control goats (P > 0.05). In both groups, PG injection is followed by a preovulatory sure in LH coincident with the FSH surge. Plasma concentrations of P decrease after the third PG injection, and the P mean concentrations in 6 hour after PG injection are significantly higher(P< 0.05)in inhibin-immunized goats than in the controls, but the overall mean concentrations after PG injection are not significantly between the two group. Plasma concentrations of E2 rise after PG-induced luteolysis to reach a peak value which is significantly (P< 0.05) higher in immunized goats than in controls. The concentrations then fall and remain relatively low. The time of peak value come later in immunized group than the control group. The conception rate is 100% in both groups. Immunized-goats produce 9 kids while only 5 in the controls. A 55% increase in kidding rate is recorded for youth goats in immunized group. The kidding rate is higher significantly between the two groups.The results demonstrate that the active immunization against inhibin enhances the secretion of FSH, E2 and P on youth dairy goat in estrous period. Immunization against inhibin induces the estrous and enhances the kidding of youth dairy goat.
引文
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[2] Hopkinson CR, Dulisch B, Gauss G, etc. The effect of local testicular irradiation on testicularhistology and plasma hormone levels in the male rat[J]. Acta Endocrinol (Copenh) ,1978, 87(2):413-23.
[3] Daume E, Chari S, Hopkinson CR, etc. lnhibin-like activity in human ovarian follicular fluid[J].Klin Wochenschr, 1978 ,Apr 1: 56(7) ,369-370.
[4] Nandini SG, Lipner H, Moudgal NR. A model system for studying inhibin[J]. Endocrinology,1976,Jun;98(6):1460-1465.
[5] Setchell BP, Sirinathsinghy DJ. Antigonadotrophic activity in rete testis fluid, a possible inhibin[J]. J Endocrmol 1972 Jun:53(3):45-55.
[6] De long FH, Sharpe RM. Evidence for inhibin-like activity in bovine follicular fluid[J]. Nature, 1976 ,Sep 2;263(5572):71-72.
[7] 叶雪清. 更年期综合证:神经--内分泌--免疫网络的变化[M],陕西科学技术出版社,1995,33-50.
[8] 李江源. 性腺疾病[M]. 天津科技翻译出版公司,1996, 65-77.
[9] 王进荣,杨利国,王元兴. 人抑制素 α 亚基主动免疫对鸡卵泡发育和性腺激素及产蛋率的影响[J]. 南京农业大学学报,2000, 23(4) :63-66.
[10] Lewis KA, Gray PC, Blount AL,etc..Betaglycan binds inhibin and can mediate functional antagonism of activin signaling[J]. Nature ,2000 ,Mar,23(404):411-414.
[11] Matzuk MM, Finegold MJ, Su JG.. Alpha-inhibin is a tumour-suppressor gene with gonadal specificity in mice[J]. Nature,1992, Nov, 26(360):313-319.
[12] Laws SC, Beggs MJ, Webbster JC. Inhibin increase and progesteronedecreases receptor for gonadotropin releasing Hormone in vitro pituitary culture[J].Endocrinology 1990 ,127:373-380.
[13] Gregg DW , schwall RH, Nett TM. Regulation of gonadotropin secretion and number of gonadotropin-release hormone receptor by inhibin activin-A and estradiol[J]. Biol.reprod,1991,44:725-732.
[14] 张小冬等. 人抑制素合成肽与猪精液抑制素提取物主动免疫对母羊生殖激素妊娠及产羔的影响[J]. 南京农业大学学报,1998,21(21)4:66- 71.
[15] Hairison CA, Farnworth PG, Chan KL, etc. Identification of specific inhibin a-binding proteins on mouse leydig and sertoli cell lines.[J]. Endocrinology, 2001, Apr, 142(4):1393-1402.
[16] Chong H, Pangas SA, Bernard DJ, etc..Structure and expression of a membrane component of the inhibin receptor system [J]. Endocrinology ,2000,Jul,141:2600-2607.
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