聚精丸对睾丸生精障碍性不育症的临床及实验研究
摘要
本研究遵循中医理论，结合临床实际，从理论、临床两方面对睾丸生精障碍性不育进行系统深入的研究。在理论研究中对该病的中医历史渊源、病因病机、辨治方法等作了系统回顾，并对西医学关于本病的病因、病理生理、诊断方法及治疗进展进行综述。实验研究部分对生精障碍性不育患者的睾丸进行了常规病理切片检查和原位末端脱氧核苷酸转移酶杂交实验(Tdt-TUNEL法，terminal deoxynucleotidyl transferase，TDT，Terminal deoxynucleotidyl transferase-mediated deoxyuride triphosphate nick-end labeling，TUNEL)，初步发现了睾丸生精障碍性不育的凋亡规律。临床研究部分对本病患者的生精细胞凋亡指数与精子质量、精子倍体进行相关分析，并对导师徐福松教授的名方聚精丸的疗效及其对患者凋亡指数的影响进行临床观察，认为聚精丸治疗后的患者生精细胞凋亡指数接近正常，有药效学意义。
Objective: To analyze the apoptotic regulation of vary types of spermatogenic cell, supporting cell and Ley dig cell in the testes of patients with infertility. To evaluate the effect of Chinese Traditional Medicine, the Pill of regenerated spermatozoa treatment, on the apoptotic change in the testes of infertility
Methods: In a prospective, nonrandomized, noncontrolled, clinical study, Testicle puncture biopsy were taken in 30 patients with dys-spermatid in the testes, and 5 cases of normal testes. Carry on the rule pathology check and the TUNEL (terminal deoxynucleotidyl transferase-mediated Dutp[deoxy-uridine triphosphate]nick end labeling) assay, (Td t ?TUNEL assay ), the changing of apoptotic indexes have been observed in vary types of spermatogenic celK supporting cell and Leydig cell in the convoluted seminiferous tubule of infertility .
Result (s): Contrasted to normal group, apoptotic indexes obviously distinct in the convoluted seminiferous tubule of infertility, mainly expressing: the apoptotic indexes were increased in primary spermatocyte and secondary spermatocyte; the apoptotic indexes decreased in spermatid and sperm, the apoptotic indexes were decreased in primary spermatocyte and secondary spermatocyte while increased in ospermatid and sperm after treating with the Pill of regenerated spermatozoa in patients; and indexes of the semen parameters were obviously improved.
Conclusion: From the apoptotic mechanism, we found that the apoptotic indexes were increased in primary spermatocyte and secondary spermatocyte while decreased in ospermatid and sperm in the testes of patients with infertility. The Pill of regenerated spermatozoa can readjusted the imbalance in spermatogenic cell with apoptotic turbulence, and obviously improved the spermatogenic function of the testes in infertilities.
Objective: To analyze the apoptotic regulation of vary types of spermatogenic cell, supporting cell and Ley dig cell in the testes of patients with infertility. To evaluate the effect of Chinese Traditional Medicine, the Pill of regenerated spermatozoa treatment, on the apoptotic change in the testes of infertility
Methods: In a prospective, nonrandomized, noncontrolled, clinical study, Testicle puncture biopsy were taken in 30 patients with dys-spermatid in the testes, and 5 cases of normal testes. Carry on the rule pathology check and the TUNEL (terminal deoxynucleotidyl transferase-mediated Dutp[deoxy-uridine triphosphate]nick end labeling) assay, (Td t ?TUNEL assay ), the changing of apoptotic indexes have been observed in vary types of spermatogenic celK supporting cell and Leydig cell in the convoluted seminiferous tubule of infertility .
Result (s): Contrasted to normal group, apoptotic indexes obviously distinct in the convoluted seminiferous tubule of infertility, mainly expressing: the apoptotic indexes were increased in primary spermatocyte and secondary spermatocyte; the apoptotic indexes decreased in spermatid and sperm, the apoptotic indexes were decreased in primary spermatocyte and secondary spermatocyte while increased in ospermatid and sperm after treating with the Pill of regenerated spermatozoa in patients; and indexes of the semen parameters were obviously improved.
Conclusion: From the apoptotic mechanism, we found that the apoptotic indexes were increased in primary spermatocyte and secondary spermatocyte while decreased in ospermatid and sperm in the testes of patients with infertility. The Pill of regenerated spermatozoa can readjusted the imbalance in spermatogenic cell with apoptotic turbulence, and obviously improved the spermatogenic function of the testes in infertilities.
引文
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2．陆仁康等．应重视和加强男性生殖健康的研究．中国男科学杂志，2001，15(1)：3
3．徐福松．不育症的中医辨证观．中医药研究，2001，17(2)：7
4．徐福松等．聚精丸治疗精液异常所致男性不育症246．江苏中医，1996，17(2)：21
5．程泾 主编．实用中西医结合不孕不育诊疗学．2000，第1版，北京：中国中医药出版社，85
6．王琦 主编．王琦男科学．1997，第1版，河南：河南科学技术出版社，339
7．岳广平，陈琼，戴宁．补肾生精汤对肾阳虚睾丸功能损害大鼠模型作用的实验研究 中国中西医结合杂志，1997，17(5)：289—291
8．金维新，李广文，孙少霞，等生精汤与液化汤治疗男性不育症临床研究．见：金之刚，陈文伯主编．男性不育与性功能障碍．北京：学苑出版社，1991．113
9．陈文怕，陈生，周书元，等．血浆睾酮水平与男性不育关系初探——128例临床病例小结．见：金之刚，陈文伯主编．男性不育与性功能障碍．北京：学苑出版社，1991．57～62
10．姚汉军．自拟益肾育精汤治疗不育52例临床观察．甘肃中医，2000，13(4)：16～17
11．刘艳芳，陈宝坤，王素君．益肾增精汤治疗精少不育症46例．山东中医杂志，2001，20(1)：25～26
12．崔文平．二仙五子生精汤治疗男性不育症．中国乡村医生，2000，15(6)：47～48
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