蓝莓花色苷提取纯化及生理功能研究
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摘要
蓝莓是杜鹃花科(Ericaceae)越橘属(Vaccinium.spp)植物,在我国有丰富的蓝莓资源。研究表明,蓝莓果中含有丰富的花色苷类物质,具有消除眼睛疲劳,保护视力,以及抗氧化、抗衰老、抗溃疡、抗炎和抗癌等生理功能和药用功能。本文通过比较4个品种蓝莓花色苷含量的差异,筛选出花色苷含量比较高的“圣云”蓝莓作为试验材料,对其花色苷进行系统的研究,以明确其生物活性,为更好地开发和利用蓝莓资源提供理论支持。
本文通过溶剂法和酶法研究蓝莓花色苷的提取工艺;研究AB-8型大孔树脂对花色苷静态和动态纯化条件,并采用聚酰胺和硅胶柱层析对花色苷进一步纯化,用硅胶薄层色谱对花色苷的组分成分进行分离。用反相高效液相色谱/质谱对蓝莓花色苷进行分离和组成成分分析;研究了蓝莓花色苷的稳定性,并对其进行微胶囊化和分子修饰;通过化学模拟试验对蓝莓花色苷的体外抗氧化作用进行了系统评价;通过动物试验研究花色苷对高脂血症大鼠血脂、抗氧化及动脉硬化的影响并探讨其作用机制。主要研究结论如下:
1.溶剂法提取蓝莓花色苷最佳条件为:60%乙醇,料液比为1g:15mL,提取时间120min,pH3.0,提取温度40℃,提取2次即可。圣云蓝莓果中花色苷的含量约为328.91mg/100g鲜果。
2.一定量的纤维素酶使蓝莓花色苷提取量提高,而果胶酶未能提高花色苷提取量,增加果胶酶用量,花色苷提取量下降。两种酶复合处理花色苷提取量低于相同用量纤维素酶的花色苷提取量,两种酶无协同作用。纤维素酶提取蓝莓花色苷的最佳条件为:酶用量5mg/g,料液比1g:8mL,酶解时间60min,pH5.0,酶解温度为45℃,提取2次即可,2次累计提取量与水提法相比,提高49.35%。
3.通过对10种大孔树脂的静态吸附解吸研究,发现AB-8型大孔树脂是一种比较理想的树脂,吸附率大,解吸率高,较适合蓝莓果中花色苷类成分的纯化。AB-8型大孔树脂对花色苷的吸附属多分子层吸附,静态吸附解吸条件为:吸附平衡时间为4h,解吸平衡时间2h,吸附平衡最适浓度为750mg/L,pH3.0时吸附能力比较强,用pH3.0的60%乙醇溶液作为解吸液,低温有利于AB-8型树脂的吸附,而高温则有利于该树脂的解吸。动态吸附解吸条件为:吸附和解吸流速1mL/min,上样液浓度3.0mg/mL,用5倍柱床体积的60%酸性乙醇作为洗脱液。经大孔树脂纯化后的蓝莓花色苷为紫黑色粉末,再经硅胶、聚酰胺柱层析,其色价分别为79.20,85.30。以硅胶G作为层析介质,通过用不同的展开剂分离蓝莓花色苷,分离效果比较好的是正丁醇-乙酸-水(4:1:5)和水-乙醇-正丁醇-乙酸乙酯(12:8:24:36)为展开剂。
4.蓝莓花色苷在酸性缓冲液和甲醇溶液中的可见区最大吸收波长分别520nm,535nm,通过高效液相色谱/质谱分析,“圣云”蓝莓含有17种花色苷单体,分别是芍药色素3-半乳糖苷、飞燕草色素3-半乳糖苷、矢车菊色素3-半乳糖苷、矢车菊色素3-葡萄糖苷、牵牛花色素3-半乳糖苷、牵牛花色素3-葡萄糖苷、矢车菊色素3-阿拉伯糖苷、芍药色素3-葡萄糖苷、锦葵色素3-半乳糖苷、锦葵色素3-葡萄糖苷、锦葵色素3-阿拉伯糖苷、牵牛花色素3-阿拉伯糖苷、飞燕草色素3-葡萄糖苷、芍药色素3-乙酰半乳糖苷、锦葵色素3-乙酰半乳糖苷、芍药色素3-乙酰葡萄糖苷、锦葵色素3-乙酰葡萄糖苷。通过制备型高效液相色谱制备2种花色苷单体,经紫外、红外、质谱、核磁共振分析鉴定,样品1主要为锦葵色素3-半乳糖苷,样品2主要为锦葵色素3-葡萄糖苷。
5.蓝莓花色苷属于水溶性色素。该花色苷在pH小于3.0条件下比较稳定,在60℃以下热稳定性较好,在短时间内有一定的耐高温能力。对光比较敏感,微波对其稳定性无不良影响。该花色苷耐氧化还原性差,抗坏血酸使花色苷稳定性下降,加速花色苷溶液的褪色。蔗糖和葡萄糖对蓝莓花色苷稳定性均无不良影响,高浓度的蔗糖和葡萄糖对其有不同程度的护色效果。苯钾酸钠对蓝莓花色苷稳定性具有良好作用。K~+不能使花色苷的吸光度增加,对花色苷的稳定性也无明显影响;Mg~(2+)、Ca~(2+)、Cu~(2+)、Al~(3+)具有增色作用,对花色苷的稳定性无显著影响:高浓度Na~+、Zn~(2+)、Mn~(2+)具有增色作用,而且能够增强花色苷的稳定性:Fe~(2+)、Fe~(3+)、Pb~(2+)对花色苷具有破坏作用,使花色苷的稳定性下降,含Fe~(3+)、Pb~(2+)的花色苷溶液出现浑浊,有沉淀生成。
6.以阿拉伯胶为壁材微胶囊化蓝莓花色苷的工艺条件为:芯材与壁材比3:197,固形物含量为20%,进风温度为180℃,出风温度为70℃。以β-环糊精-麦芽糊精-阿拉伯胶为壁材微胶囊化蓝莓花色苷的工艺条件为:芯材与壁材比3:97,β-环糊精:麦芽糊精为1:2,阿拉伯胶占总固形物的百分比为7.5%,固形物含量为20%。以大豆分离蛋白-麦芽糊精为壁材对蓝莓花色苷进行微胶囊化的工艺条件:大豆分离蛋白与麦芽糊精比为3:7,芯材:壁材为4:96,固形物含量为15%。乙基纤维素作为壁材微胶囊化蓝莓花色苷的工艺条件为聚乙二醇含量15%,芯材与壁材比1:19,固形物含量4%。微胶囊产品在模拟胃液环境中释放率比较高,在模拟胃液环境中停留60min后,释放率均达到90%以上。蓝莓花色苷微胶囊化和酰化后,花色苷的光和热稳定性提高。
7.蓝莓花色苷具有抗油脂和抗脂质体氧化能力,而且抗脂质体氧化能力强于抗坏血酸。蓝莓花色苷具有较强的还原能力:蓝莓花色苷具有清除羟基自由基、超氧阴离子自由基、DPPH自由基和H_2O_2能力。
8.与高脂模型组相比,灌胃蓝莓花色苷能显著降低大鼠血清的总甘油三酯(TG)、总胆固醇(TC)、低密度脂蛋白胆固醇(LDL-C)水平和载脂蛋白ApoB含量,显著提高高密度脂蛋白胆固醇(HDL-C)水平和载脂蛋白ApoAⅠ含量,动脉硬化指数显著下降。与高脂模型组相比,灌胃蓝莓花色苷可以有效提高大鼠血清和肝脏总抗氧化能力,抗氧化酶SOD和GSH-Px活性显著提高,脂质过氧化物MDA含量显著下降。结果表明:蓝莓花色苷能够降低高脂血症大鼠血脂水平,改善血脂代谢,抑制脂质过氧化,可以有效的预防动脉硬化(AS)的发生和发展。
Blueberry is abundant and distributing over the country.Anthocyanins have been discussed in relation to a wide range of function characteristic such as vision improvement, antioxidant,antidecrepitude,antiulcer,antivirus and anticancer and so on.In this paper, Saintcloud blueberry with most anthocyanin content was selected after comparing anthocyanin content of 4 kinds of blueberry fruits.Anthocyanins were systematically studied from Saintcloud blueberry as material.It is very important to understand its bioactivities and use the blueberry resource.
In this paper,anthocyanins were extracted from Saitcloud blueberry fruits.The optimum industrial process of extraction of blueberry anthocyanins were determined by solvent and enzyme method.Blueberry anthocyanins' conditions of absorption and desorption which were static and dynamic were studied by AB-8 macroporous resin.Blueberry anthocyanins were purified by polyamide and silica-gel column chromatography.Blueberry anthocyanins were separated by thin-layer chromatography.Constitutes and structrue of blueberry anthocyanins was identified by RP-HPLC/MS.The stability of blueberry anthocyanins were tested.Microencapsulation and molecular modification of blueberry anthoeyanins were studied.The effect of blueberry anthocyanins on antioxidant was investigated by the method of chemical simulation in vitro.The effects of anthocyanions from blueberry fruits on hypolipidemic and antioxidative in hyperlipidemic rats were studied by animal tests.The main results were as follows:
1.The optimum extraction technology parameters determined by ethanol were as follows: 60%ethanol,material-solvent ratio 1g:15mL,extraction time 120min,pH value 3.0, extraction temperature 40℃and extraction 2 times.Anthocyanins content was 328.91 mg/100g fresh fruits.
2.The effect of cellulase,pectinase and their mixture on blueberry anthocyanins extraction was studied.The result showed that cellulose could enhance the extraction content of anthocyanins,but pectinse could not.In addition,the extraction content of anthocyanins by their mixture was lower than by cellulase.The optimum parameters of extraction technology by cellulase were enzymatic quantify 5mg/g,material-liquid ratio 1 g:8mL,enzymolysis time 60min,pH value 5.0,enzymolysis temperature 45℃and extraction 2 times.Blueberry anthocyanins content was more 49.53%by cellulase on extraction than water.
3.Among 10 kinds of macroporous resins,AB-8 resin has better capabilities of absorption and desorption to purify crude blueberry anthocyanins.The conditions of static absorption and desorption of blueberry anthocyanins were that anthocyanins concentration in balance was 750mg/L,pH value of absorption was 3.0,the elution solvent was pH3.0 and 60%ethanol.The optimum conditions of dynamic absorption and desorption of blueberry anthocyanins were as follows:flow rate of absorption and desorption 1mL/min,concentration 3.0mg/mL.The elution solvent was 3BV(bed volume)60%ethanol.In those conditions,the anthocyanins produced by this technological process was purplish dark powder.Blueberry anthocyanins were purified by silica-gel and polyamide column chromatography after AB-8 resin purifying,the colour value was 79.20,85.30 respectively.Anthocyanins were separated by thin-layer chromatography.Separation was well done by butanol-acetic-water(4:1:5)and water-ethanol- butanol - acetic ester(12:8:24:36) as developing solvent.Two kinds of anthocyanins were isolated by preparative HPLC.Sample 1 was Malvidin3-arabinoside, Sample 2 was Malvidin3-glucoside by UV,IR,MS and NMR.
4.The wavelength of absorption respectively was 520nm and 535nm in pH3.0 citric acid solvent and methanol.Saintcloud blueberry was found to contain 17 anthocyanins by HPLC/MS.The 17 of them were tentatively identified as Peonidin3-galactoside, Delphinidin3-galactosid,Cyanidin3-galactoside,Cyanidin3-glucoside,Petunidin3-galactoside ,Petunidin3-glucoside,Cyanidin3-arabinoside,Peonidin3-glucoside,Malvidin3-galactoside,Mal -vidin3-glucoside,Malvidin3-galactoside,Petunidin3-arabinoside,Delphinidin3-glucoside,Peon -idin3-(6"-acetyl)galactoside,Malvidin3-(6"-acetyl)galactoside,Peonidin3-(6"-acetyl) glucoside,Malvidin3-(6"-acetyl)glucoside.
5.Blueberry anthocyanins are water-soluble pigments.The anthocyanins were stable under pH 3.0,below 60℃.It was heat-resistant to high temperature in a short time.The anthocyanins were sensitive to light and stable to microwave.It was found that oxidizer H_2O_2, reductive Na_2SO_3 and ascorbic acid could decrease the stability of anthocyanins and result in its more rapid discoloration.Sucrose and glucose had no harmful effect on the stability of blueberry anthocyanins,but had certain color maintenance at higher concentration.Sodium benzoate and K~+ had no effect on the stability of anthocyanins.Mg~(2+),Ca~(2+),Cu~(2+)and A1~(3+) had a certain effect of clout improvement,but no effect on the stability of anthocyanins.Na~+,Zn~(2+) and Mn~(2+)could not holy strengthen the colour at higher concentration,but enhance the stability of blueberry anthocyanins.Fe~(2+),Fe~(3+) and Pb~(2+) were harmful to the stability of blueberry anthocyanins while Fe~(3+) and Pb~(2+) could lead to precipitate in blueberry anthocyanins solution.
6.The optimum technology parameters by using arabic gum as wall materials were as follows:the proportion of anthocyanins to wall materials was 3:197,emulsion concentration was 20%,inlet air and outlet air temperature were 180℃and 70℃respectively.The optimum technology parameters by using mixture ofβ-cyclodextrin,maltodextrinn,and arabic gum as wall materials were as follows:the ratio of core mateials to wall mateials 3:97,β-cyclodextfinto maltodextriun 1:2,arabic gum 7.5%,emulsion concentration 20%.The optimum technology parameters with mixture of soybean protein isolatidn(SPI) and maltodextrin showed that SPI and maltodextrin was in proportion of 3:7 as the materials,the proportion of core mateials to wall mateials was 4:96,the emulsion concentration was 15%(W/V).The optimum technology parameters by using ethyl cellulose as wall materials were as follows:EPG concentration was 15%,the proportion of anthocyanins to wall materials was 1:19,emulsion concentration was 4%respectively.Anthocyanins of microencapsulation have higher releasing rate in vitro.Releasing rate could exceed 90%after 60min in gastric juice of simulation.The temperature and light stability of microencapsulation and anthocyanins of molecular modification could be improved.
7.Blueberry anthocyanins could inhibit peroxidation of lard and lipid,and have stronger effect of lipid peroxidation than ascorbic acids.Blueberry anthocyanins have the reduction ability,and the positive correlation existed between reductive ability and concentration. Blueberry anthoeyanins could effectively scavenge hydroxyl free radicals,superoxide anions, DPPH and peroxide.
8.The serum TG,TC,LDL-C concentration,ApoB content and atherosclerosis index in rats fed with anthocyanins from blueberry fruits(ABBF)were significantly lower than those in model group,while serum HDL-C concentration and ApoA I content were higher.Comparing with model group,T-AOC,SOD,GSH-Px were increased significantly in serum and liver by feeding with ABBF,while MDA content were decreased in serum and liver.ABBF could decrease blood lipids level,improve blood lipid metabolism,inhibit lipid peroxidation,inhibit formation and development of antherosclerosis.
本文通过溶剂法和酶法研究蓝莓花色苷的提取工艺;研究AB-8型大孔树脂对花色苷静态和动态纯化条件,并采用聚酰胺和硅胶柱层析对花色苷进一步纯化,用硅胶薄层色谱对花色苷的组分成分进行分离。用反相高效液相色谱/质谱对蓝莓花色苷进行分离和组成成分分析;研究了蓝莓花色苷的稳定性,并对其进行微胶囊化和分子修饰;通过化学模拟试验对蓝莓花色苷的体外抗氧化作用进行了系统评价;通过动物试验研究花色苷对高脂血症大鼠血脂、抗氧化及动脉硬化的影响并探讨其作用机制。主要研究结论如下:
1.溶剂法提取蓝莓花色苷最佳条件为:60%乙醇,料液比为1g:15mL,提取时间120min,pH3.0,提取温度40℃,提取2次即可。圣云蓝莓果中花色苷的含量约为328.91mg/100g鲜果。
2.一定量的纤维素酶使蓝莓花色苷提取量提高,而果胶酶未能提高花色苷提取量,增加果胶酶用量,花色苷提取量下降。两种酶复合处理花色苷提取量低于相同用量纤维素酶的花色苷提取量,两种酶无协同作用。纤维素酶提取蓝莓花色苷的最佳条件为:酶用量5mg/g,料液比1g:8mL,酶解时间60min,pH5.0,酶解温度为45℃,提取2次即可,2次累计提取量与水提法相比,提高49.35%。
3.通过对10种大孔树脂的静态吸附解吸研究,发现AB-8型大孔树脂是一种比较理想的树脂,吸附率大,解吸率高,较适合蓝莓果中花色苷类成分的纯化。AB-8型大孔树脂对花色苷的吸附属多分子层吸附,静态吸附解吸条件为:吸附平衡时间为4h,解吸平衡时间2h,吸附平衡最适浓度为750mg/L,pH3.0时吸附能力比较强,用pH3.0的60%乙醇溶液作为解吸液,低温有利于AB-8型树脂的吸附,而高温则有利于该树脂的解吸。动态吸附解吸条件为:吸附和解吸流速1mL/min,上样液浓度3.0mg/mL,用5倍柱床体积的60%酸性乙醇作为洗脱液。经大孔树脂纯化后的蓝莓花色苷为紫黑色粉末,再经硅胶、聚酰胺柱层析,其色价分别为79.20,85.30。以硅胶G作为层析介质,通过用不同的展开剂分离蓝莓花色苷,分离效果比较好的是正丁醇-乙酸-水(4:1:5)和水-乙醇-正丁醇-乙酸乙酯(12:8:24:36)为展开剂。
4.蓝莓花色苷在酸性缓冲液和甲醇溶液中的可见区最大吸收波长分别520nm,535nm,通过高效液相色谱/质谱分析,“圣云”蓝莓含有17种花色苷单体,分别是芍药色素3-半乳糖苷、飞燕草色素3-半乳糖苷、矢车菊色素3-半乳糖苷、矢车菊色素3-葡萄糖苷、牵牛花色素3-半乳糖苷、牵牛花色素3-葡萄糖苷、矢车菊色素3-阿拉伯糖苷、芍药色素3-葡萄糖苷、锦葵色素3-半乳糖苷、锦葵色素3-葡萄糖苷、锦葵色素3-阿拉伯糖苷、牵牛花色素3-阿拉伯糖苷、飞燕草色素3-葡萄糖苷、芍药色素3-乙酰半乳糖苷、锦葵色素3-乙酰半乳糖苷、芍药色素3-乙酰葡萄糖苷、锦葵色素3-乙酰葡萄糖苷。通过制备型高效液相色谱制备2种花色苷单体,经紫外、红外、质谱、核磁共振分析鉴定,样品1主要为锦葵色素3-半乳糖苷,样品2主要为锦葵色素3-葡萄糖苷。
5.蓝莓花色苷属于水溶性色素。该花色苷在pH小于3.0条件下比较稳定,在60℃以下热稳定性较好,在短时间内有一定的耐高温能力。对光比较敏感,微波对其稳定性无不良影响。该花色苷耐氧化还原性差,抗坏血酸使花色苷稳定性下降,加速花色苷溶液的褪色。蔗糖和葡萄糖对蓝莓花色苷稳定性均无不良影响,高浓度的蔗糖和葡萄糖对其有不同程度的护色效果。苯钾酸钠对蓝莓花色苷稳定性具有良好作用。K~+不能使花色苷的吸光度增加,对花色苷的稳定性也无明显影响;Mg~(2+)、Ca~(2+)、Cu~(2+)、Al~(3+)具有增色作用,对花色苷的稳定性无显著影响:高浓度Na~+、Zn~(2+)、Mn~(2+)具有增色作用,而且能够增强花色苷的稳定性:Fe~(2+)、Fe~(3+)、Pb~(2+)对花色苷具有破坏作用,使花色苷的稳定性下降,含Fe~(3+)、Pb~(2+)的花色苷溶液出现浑浊,有沉淀生成。
6.以阿拉伯胶为壁材微胶囊化蓝莓花色苷的工艺条件为:芯材与壁材比3:197,固形物含量为20%,进风温度为180℃,出风温度为70℃。以β-环糊精-麦芽糊精-阿拉伯胶为壁材微胶囊化蓝莓花色苷的工艺条件为:芯材与壁材比3:97,β-环糊精:麦芽糊精为1:2,阿拉伯胶占总固形物的百分比为7.5%,固形物含量为20%。以大豆分离蛋白-麦芽糊精为壁材对蓝莓花色苷进行微胶囊化的工艺条件:大豆分离蛋白与麦芽糊精比为3:7,芯材:壁材为4:96,固形物含量为15%。乙基纤维素作为壁材微胶囊化蓝莓花色苷的工艺条件为聚乙二醇含量15%,芯材与壁材比1:19,固形物含量4%。微胶囊产品在模拟胃液环境中释放率比较高,在模拟胃液环境中停留60min后,释放率均达到90%以上。蓝莓花色苷微胶囊化和酰化后,花色苷的光和热稳定性提高。
7.蓝莓花色苷具有抗油脂和抗脂质体氧化能力,而且抗脂质体氧化能力强于抗坏血酸。蓝莓花色苷具有较强的还原能力:蓝莓花色苷具有清除羟基自由基、超氧阴离子自由基、DPPH自由基和H_2O_2能力。
8.与高脂模型组相比,灌胃蓝莓花色苷能显著降低大鼠血清的总甘油三酯(TG)、总胆固醇(TC)、低密度脂蛋白胆固醇(LDL-C)水平和载脂蛋白ApoB含量,显著提高高密度脂蛋白胆固醇(HDL-C)水平和载脂蛋白ApoAⅠ含量,动脉硬化指数显著下降。与高脂模型组相比,灌胃蓝莓花色苷可以有效提高大鼠血清和肝脏总抗氧化能力,抗氧化酶SOD和GSH-Px活性显著提高,脂质过氧化物MDA含量显著下降。结果表明:蓝莓花色苷能够降低高脂血症大鼠血脂水平,改善血脂代谢,抑制脂质过氧化,可以有效的预防动脉硬化(AS)的发生和发展。
Blueberry is abundant and distributing over the country.Anthocyanins have been discussed in relation to a wide range of function characteristic such as vision improvement, antioxidant,antidecrepitude,antiulcer,antivirus and anticancer and so on.In this paper, Saintcloud blueberry with most anthocyanin content was selected after comparing anthocyanin content of 4 kinds of blueberry fruits.Anthocyanins were systematically studied from Saintcloud blueberry as material.It is very important to understand its bioactivities and use the blueberry resource.
In this paper,anthocyanins were extracted from Saitcloud blueberry fruits.The optimum industrial process of extraction of blueberry anthocyanins were determined by solvent and enzyme method.Blueberry anthocyanins' conditions of absorption and desorption which were static and dynamic were studied by AB-8 macroporous resin.Blueberry anthocyanins were purified by polyamide and silica-gel column chromatography.Blueberry anthocyanins were separated by thin-layer chromatography.Constitutes and structrue of blueberry anthocyanins was identified by RP-HPLC/MS.The stability of blueberry anthocyanins were tested.Microencapsulation and molecular modification of blueberry anthoeyanins were studied.The effect of blueberry anthocyanins on antioxidant was investigated by the method of chemical simulation in vitro.The effects of anthocyanions from blueberry fruits on hypolipidemic and antioxidative in hyperlipidemic rats were studied by animal tests.The main results were as follows:
1.The optimum extraction technology parameters determined by ethanol were as follows: 60%ethanol,material-solvent ratio 1g:15mL,extraction time 120min,pH value 3.0, extraction temperature 40℃and extraction 2 times.Anthocyanins content was 328.91 mg/100g fresh fruits.
2.The effect of cellulase,pectinase and their mixture on blueberry anthocyanins extraction was studied.The result showed that cellulose could enhance the extraction content of anthocyanins,but pectinse could not.In addition,the extraction content of anthocyanins by their mixture was lower than by cellulase.The optimum parameters of extraction technology by cellulase were enzymatic quantify 5mg/g,material-liquid ratio 1 g:8mL,enzymolysis time 60min,pH value 5.0,enzymolysis temperature 45℃and extraction 2 times.Blueberry anthocyanins content was more 49.53%by cellulase on extraction than water.
3.Among 10 kinds of macroporous resins,AB-8 resin has better capabilities of absorption and desorption to purify crude blueberry anthocyanins.The conditions of static absorption and desorption of blueberry anthocyanins were that anthocyanins concentration in balance was 750mg/L,pH value of absorption was 3.0,the elution solvent was pH3.0 and 60%ethanol.The optimum conditions of dynamic absorption and desorption of blueberry anthocyanins were as follows:flow rate of absorption and desorption 1mL/min,concentration 3.0mg/mL.The elution solvent was 3BV(bed volume)60%ethanol.In those conditions,the anthocyanins produced by this technological process was purplish dark powder.Blueberry anthocyanins were purified by silica-gel and polyamide column chromatography after AB-8 resin purifying,the colour value was 79.20,85.30 respectively.Anthocyanins were separated by thin-layer chromatography.Separation was well done by butanol-acetic-water(4:1:5)and water-ethanol- butanol - acetic ester(12:8:24:36) as developing solvent.Two kinds of anthocyanins were isolated by preparative HPLC.Sample 1 was Malvidin3-arabinoside, Sample 2 was Malvidin3-glucoside by UV,IR,MS and NMR.
4.The wavelength of absorption respectively was 520nm and 535nm in pH3.0 citric acid solvent and methanol.Saintcloud blueberry was found to contain 17 anthocyanins by HPLC/MS.The 17 of them were tentatively identified as Peonidin3-galactoside, Delphinidin3-galactosid,Cyanidin3-galactoside,Cyanidin3-glucoside,Petunidin3-galactoside ,Petunidin3-glucoside,Cyanidin3-arabinoside,Peonidin3-glucoside,Malvidin3-galactoside,Mal -vidin3-glucoside,Malvidin3-galactoside,Petunidin3-arabinoside,Delphinidin3-glucoside,Peon -idin3-(6"-acetyl)galactoside,Malvidin3-(6"-acetyl)galactoside,Peonidin3-(6"-acetyl) glucoside,Malvidin3-(6"-acetyl)glucoside.
5.Blueberry anthocyanins are water-soluble pigments.The anthocyanins were stable under pH 3.0,below 60℃.It was heat-resistant to high temperature in a short time.The anthocyanins were sensitive to light and stable to microwave.It was found that oxidizer H_2O_2, reductive Na_2SO_3 and ascorbic acid could decrease the stability of anthocyanins and result in its more rapid discoloration.Sucrose and glucose had no harmful effect on the stability of blueberry anthocyanins,but had certain color maintenance at higher concentration.Sodium benzoate and K~+ had no effect on the stability of anthocyanins.Mg~(2+),Ca~(2+),Cu~(2+)and A1~(3+) had a certain effect of clout improvement,but no effect on the stability of anthocyanins.Na~+,Zn~(2+) and Mn~(2+)could not holy strengthen the colour at higher concentration,but enhance the stability of blueberry anthocyanins.Fe~(2+),Fe~(3+) and Pb~(2+) were harmful to the stability of blueberry anthocyanins while Fe~(3+) and Pb~(2+) could lead to precipitate in blueberry anthocyanins solution.
6.The optimum technology parameters by using arabic gum as wall materials were as follows:the proportion of anthocyanins to wall materials was 3:197,emulsion concentration was 20%,inlet air and outlet air temperature were 180℃and 70℃respectively.The optimum technology parameters by using mixture ofβ-cyclodextrin,maltodextrinn,and arabic gum as wall materials were as follows:the ratio of core mateials to wall mateials 3:97,β-cyclodextfinto maltodextriun 1:2,arabic gum 7.5%,emulsion concentration 20%.The optimum technology parameters with mixture of soybean protein isolatidn(SPI) and maltodextrin showed that SPI and maltodextrin was in proportion of 3:7 as the materials,the proportion of core mateials to wall mateials was 4:96,the emulsion concentration was 15%(W/V).The optimum technology parameters by using ethyl cellulose as wall materials were as follows:EPG concentration was 15%,the proportion of anthocyanins to wall materials was 1:19,emulsion concentration was 4%respectively.Anthocyanins of microencapsulation have higher releasing rate in vitro.Releasing rate could exceed 90%after 60min in gastric juice of simulation.The temperature and light stability of microencapsulation and anthocyanins of molecular modification could be improved.
7.Blueberry anthocyanins could inhibit peroxidation of lard and lipid,and have stronger effect of lipid peroxidation than ascorbic acids.Blueberry anthocyanins have the reduction ability,and the positive correlation existed between reductive ability and concentration. Blueberry anthoeyanins could effectively scavenge hydroxyl free radicals,superoxide anions, DPPH and peroxide.
8.The serum TG,TC,LDL-C concentration,ApoB content and atherosclerosis index in rats fed with anthocyanins from blueberry fruits(ABBF)were significantly lower than those in model group,while serum HDL-C concentration and ApoA I content were higher.Comparing with model group,T-AOC,SOD,GSH-Px were increased significantly in serum and liver by feeding with ABBF,while MDA content were decreased in serum and liver.ABBF could decrease blood lipids level,improve blood lipid metabolism,inhibit lipid peroxidation,inhibit formation and development of antherosclerosis.
引文
1.鲍鲁生.1999.食品工业中应用的微胶囊技术.食品科学,20(10):6-9
2.毕红霞,李建伟,陈玮,等.AB-8吸附树脂对欧李色素的吸附和精制.郑州工程学院学报,2004,25(2):40-42
3.蔡俊.2005.酶在番茄红素提取过程中的应用研究.湖北工业大学学报,20(1):8-10
4.曹炜,尉亚辉,郭斌.2002.用化学发光法研究蜂胶对氧自由基的清除作用,光子学报,31(2):162-164
5.陈炳华,刘剑秋.2003.不同溶剂和保存条件对尾叶悬钩子花色素苷提取及稳定性的影响.植物资源与环境学报,12(4):11-15
6.陈丛瑾,黄克瀛,张东峰,等.2006.超声波提取白英果红色素的工艺研究.食品研究与开发,27(8):80-83
7.陈建初,叶兴乾.2005.抗坏血酸对杨梅花色苷色素稳定性的影响.浙江大学学报(农业与生命科学版),31(3):298-300
8.陈连文,刘敬兰,师建华.2001.黑糯玉米色素的提取及稳定性研究.食品科技,27(1):39-41
9.陈留勇,孟宪军,贾薇,等.2004.黄桃水溶性多糖的抗肿瘤作用及清除自由基、提高免疫活性研究食品科学,25(2):167-170
10.陈伟,孟宪军,王立男.2006.玫瑰花红色素提取工艺研究.食品研究与开发,27(3):50-52
11.陈晓辉,谭晓杰,田中克佳.2004.制备型高效液相色谱在中药野菊花化学成分分离中的应用.药品评价,1(5):359-361
12.陈勇,张晴.2001.AB-8大孔吸附树脂吸附和分离紫甘薯色素的研究.中国食品添加剂,(1):6-9
13.陈勇,赵春芬,孙清,等.1998.紫苏色素稳定性的初步研究.中国食品添加剂,(3):4-8
14.陈志华,李静.2000.粉状脂溶性茶多酚在色拉油中的性能实验.中国油脂,25:55-57
15.程超,莫开菊,汪兴平.2005.天然水溶性火棘色素在不同环境条件下的稳定性研究.食品科学,26(3):72-74
16.程瑛琨,李蕾,王丹梅,等.2006.酸浆宿萼色素的提取及稳定性的研究.食品工业科技,27(12):58-64
17.程元恺.1993.脂质过氧化与抗氧化酶.工业卫生与职业病,13(4):254
18.崔僖山编绎.1992.食品文摘,2:9-11
19.党蕊叶,权清转.2005.大孔吸附树脂纯化矮牵牛化红色素.西北农业学报,14(3):145-147,158
20.段翰英,李远志,黄苇,等.2004.微胶囊Vc加工方法及性质研究.食品工业科技,25(8):107-108
21.樊金玲,丁霄霖.2006.沙棘籽提取物抗氧化活性与酚类组成的研究.天然产物研究与开发,18(4):529-524,554
22.范润珍,韩少伟,林宏图.2006.山竹壳色素的提取及其稳定性研究.食品科学,27(8):358-362
23.方允中,郑荣梁主编.2002.自由基生物学的理论与应用.北京:科学出版社,60-69
24.冯卫华,刘邻渭,许克勇.2004.猕猴桃籽油微胶囊化技术研究.农业工程学报,20(1):234-237
25.葛毅强.2000.微胶囊型天然维生素E粉末的研制.食品工业,(3):32-34
26.顾红梅,张新申,蒋小平.2004.超声波法和冻结-融解法相结合提取紫薯中花色甙.食品科学,25(7):104-107
27.顾文秀,谢为明,夏文水.2005.超声波提取乌饭树叶色素及其稳定性的研究.林产化学与工 业,25(4):74-78
28.郭红,陈在嘉,徐义枢.1991.血清脂质与脂质过氧化关系的临床研究.中国循环杂志,6(5):373-375
29.郭颖.2001.微胶囊化左旋肉碱.江南大学硕士论文,10
30.郝建军,刘延吉.2001.植物生理实验技术.沈阳:辽宁科学技术出版社.
31.何耀,李良涛,万志恒.冠心病血清载脂蛋白A1和B100的观察.中华心血管病杂志,1990,18(4):217-219
32.胡天喜.1997.自由基生命科学进展(第5集).北京:原子能出版社,66-67
33.黄涛,卢起来.1997.月季花红色素的提取与性质研究.精细化学,14(5):21-23
34.黄延春,索全伶,贺文智,等.2005.天然实用红木色素的分离纯化与结构表征.天然产物研究与开发,17(6):681-684
35.回瑞华,侯冬严.1995.近代有机仪器分析.吉林:吉林大学出版社
36.霍琳琳,苏平,吕英华.2005.分光光度法测定桑葚总花色苷含量的研究.酿酒,23(4):88-89
37.姜平平,吕晓玲,姚秀玲,等.2002.紫心甘薯花色苷抗氧化活性体外实验研究.中国食品添加剂,(6):8-11
38.姜平平,吕晓玲朱惠丽.2003.花色苷类物质分离鉴定方法.中国食品添加剂,(4):108-111
39.焦中高,刘杰超,王思新.2003.黑莓红色素抗氧化活性的研究.食品科技,(8):63-65
40.金呈之.2006.紫草色素制备的研究进展.江苏食品发酵,(2):16-20
41.金锋,李新华,张森.2006.玉米色素微胶囊制备工艺研究.中国食品添加剂,48-52
42.金杰,李志西,张锋,等.2006.桑椹醋提取物对二苯代苦味酰基自由基(DPPH·)的清除作用.西北农林科技大学学报(自然科学版),35(3):135-137
43.金宁,刘通迅.2007.山楂原花青素的抗氧化活性.食品与发酵工业,33(1):45-47
44.李春阳.2006.葡萄籽中原花青素的提取纯化及其结构和功能研究.江南大学博士论文.
45.李大婧,刘春泉,方桂珍.2007.酶为介质提取万寿菊花中叶黄素的工艺研究.农业工程学报,23(5):232-236
46.李剑君,李稳宏.2001.葛根总黄酮中葛根素的分离研究.西北大学学报(自然科学版),34(4):311-314
47.李进,彭宇,彭子模.2004.鸡冠花红色素理化性质及其稳定性研究.生物技术,14(1):21-24
48.李进.2005.大孔树脂吸附分离黑果枸杞色素的研究.食品科学,26(6):47-51
49.李军,徐本明,刘珂.2004.曲克卢丁对照品的制备液相色谱分离.中国医药工业杂志,35:285-287
50.李善吉,伍胜君.2005.大孔树脂的应用研究概况.广东轻工职业技术学院学报,4(2):11-13
51.李书国,薛文通,李雪梅,等.2005.乙基纤维素微胶囊化Vc工艺参数及其活性保护的研究.食品工业科技,26(5):143-148
52.李亚东等.2001.中国蓝莓产业化发展的前景.沈阳农业大学学报,3(1):39-42
53.李亚东.2002.蓝莓果实的成分及保健机能.中国食品与营养,(1):27-285
4.李永强,张玉娜,王明珍,等.2005.白刺果实色素的提取及理化性质研究.山东农业大学学报(自然科学版),36(1):75-81
55.励建荣,岑沛霖,Joyce D C.2002.杨梅汁内花色苷热降解动力学研究.科技通报,18(1):1-5
56.梁少玲,蔡宇,杨燕霞,等.2006.大孔树脂吸附在黄酮及皂苷类成分分离纯化中的应用.时珍国医国 药,17(2):277-278
57.梁治齐.1999.微胶囊技术及其应用.中国轻工业出版社
58.林秋风,王凌云,李药兰,等.2003.AB-8大孔树脂分离长瓣金莲花总黄酮.暨南大学学报(自然科学版),24(1):55-58
59.凌关庭.2003a.食品添加剂手册(第三版).北京:化学工业出版社,685-690
60.凌关庭.2003b.可供开发食品添加剂(Ⅸ):兰莓提取物及其抗氧化作用.粮食与油脂,(6):45-48.
61.凌关庭.2004.抗氧化食品与健康.北京:化学工业出版社(第一版),1-58,233-234
62.刘斌,石任兵,余超.2002.影响大孔树脂吸附分离中草药化学成分的因素.中草药,33(5):475-476
63.刘富梁,梁华正,刘成佐,等.大孔树脂分离纯化商陆红色素的研究.化工时刊,2006,20(1):33-35
64.刘广文.2001.喷雾干燥实用技术大全.北京:中国轻工业出版社
65.刘建新,胡浩斌,赵国林.2006.叶子花红色素的提取及其稳定性研究.食品科学,27(8):98-102
66.刘杰超,焦中高,周红平,等.2006.甜樱桃红色素的体外抗氧化活性.果树学报,23(5):756-759
67.刘邻渭.2000.食品化学.北京:中国农业出版社.
68.刘玲,李霞,金同铭.1998.高压液相色谱在花色素苷分析中的应用.北京农业科学,16(2):30-33
69.刘小珍.2002.脐橙色素的稳定性.光谱实验室,19(6):784-785
70.刘云海,刘瑛,曹小红,等.2004.天然食用色素花青素的微胶囊化.食品工业科技,25(12):109-110
71.卢蓉蓉,张国农.2001.喷雾干燥微胶囊技术中的玻璃化转变.中国乳品工业,6:24-27
72.卢钰,董现义,杜景平.2005.花色苷研究进展.山东农业大学学报,35(2):315-320
73.陆国权,蓝小明,楼晓波,等.1996.紫心甘薯红色素理化性质的研究.浙江农业大学学报.22(3):308-301
74.陆国权,吴小蓉.1997.黑豆皮色素的提取及其理化性质研究.中国粮油学报.12(3):53-58
75.吕晓玲,孔德莉,姚秀玲,等.2006.采用荧光化学发光法研究甜菜红色素的抗氧化作用.食品与发酵工业,32(10):70-73.
76.马银海,彭永芳,张永丽,等.2000.AB-8树脂吸附和分离黑糯米黑色素.食品科学,21(12):93-94
77.马银海,彭永芳,付云海,等.2005a.一串红色素的提取工艺研究.食品科学,26(9):311-313
78.马银海,彭永芳,杨丽琳,等.2005b.草莓红色素的提取及树脂吸附.食品科学,26(7):56-58
79.孟红梅,韩多红,李彩霞,等.2006.唐古特白刺果实红色素稳定性研究.食品科学,27(8):72-75
80.孟洁.黑大豆色素的研究.2000.青岛大学学报,13(2):29-33
81.孟志芬,董彩霞,孟现超.2005.酶法提取芝麻叶中总黄酮的工艺研究郑州大学学报(工学版):26(4):53-56
82.莫开菊,柳圣,程超.2006.生姜黄酮的抗氧化活性研究.食品科学,27(9):110-115
83.聂芊,吴春.2002.辅色素对笃柿天然色素稳定性的影响.食品研究与开发,23(3):31-32
84.帕提古丽,马合木提,高莉.2004.酸浆花萼色素的提取及理化性质研究.食品科学.25(9):35-38
85.潘见,陈强,谢慧明,等.1999.大孔树脂对葛根黄酮的吸附分离特性研究.农业工程学报,15(1):236
86.庞战军,周玫,陈瑗.2000.自由基医学研究方法.北京:人民卫生出版社,23-115
87.祁建军,宋经元,雷和田,等.2002.根癌农杆菌转化紫草的研究.云南植物研究,24(5):656-658
88.乔华,张生万,李美萍,等.2006.天然色素稳定性研究及其新的类型划分.食品科学,27(9):69-73
89.钦传光,马丽,丁焰,等.1998.纸色谱法鉴定蛇莓红色素的成分.湖北工学院学报,13(3):5-8
90.邱业先,王桃云.2002.野菊花黄色素提取工艺研究.食品与发酵工业,28(3):33-36
91.任海,查丽杭,秦川,等.2002.大孔树脂分离提取麻黄碱的研究.离子交换与吸附,18(2):97
92.上官新晨,沈勇根,徐明生,等.2006.大孔树脂对紫红薯色素吸附和解吸的特性研究.食品科技,(7):117-120
93.尚庆坤,祝红,阎吉昌.1999.高效制备液相色谱法分离制备虻中的多肽样品.分析化学研究简报,27(8):924-926
94.宋常春,王晓鹏.2003.壳聚糖膜的制备及对植物色素富集作用的研究.食品科学,24(10):88-90
95.苏金为.1999.黑米色素的提取和组成分析.福建农业大学学报,28(2):233-237
96.孙贵宝.2002.越橘的保健作用及各国栽培发展趋势.农机化研究,(3):225
97.孙健,蒋跃明,彭子模.2005.利用大孔吸附树脂提取蜀葵花色素的研究.生物技术,15(1):63-65
98.孙庆杰,顾琼芬.1997.超临界萃取技术在天然色素中的应用.农牧产品开发,(12);3-5
99.孙艳华,苗靖.2006.酶法提取荞麦茎叶中黄酮的研究.食品工业,(3):16-18
100.孙阳昭,孙志奸,廖小军,等.2006.红莓果中花青素的提取工艺研究.食品与发酵工业,32(1):129-133
101.孙志健,张燕,陈芳,等.2005.对蓝莓产业化发展的思考.食品工业科技,26(12):183-184
102.唐传核,马青.1998.花色苷色素以及其在食品中的应用.中国食品用化学品,(6):29-31
103.唐传核.2005.植物生物活性物质.北京:化学工业出版社.247
104.唐璇,李稳红,李新生,等.2006.黄姜中黄色素的提取工艺研究.食品科学,27(10):337-340
105.王冬梅,尉芹,马希汉.2002.大孔树脂在药用植物有效成分分离中的应用,西北林学院学报,17(1):60-63
106.王晖,刘佳佳.2003.银杏黄酮的酶法提取工艺研究.中药材,26(12):887-888
107.王慧琴,谢明勇,付志红.2003.红花红色素的抗氧化活性.无锡轻工大学学报,22(5):98-101
108.王可兴,潘思轶,罗锐.2003.原花青素微胶囊工艺及稳定性研究.食品科学,24(8):88-90.
109.王克勤.1995.脂蛋白与动脉粥样硬化.北京:人民卫生出版社,417
110.王力生,邹节明,郭亚健.2004.D101大孔树脂纯化苦玄参总皂苷的研究.中草药,35(5):515-517
111.王琳芳,杨克恭.2001.医学分子生物学原理.北京:高等教育出版社,956-957
112.王琳琳,凌文华,马静,等.2002.膳食黑米皮抑制高脂诱导家兔动脉粥样硬化形成及其机制研究.营养学报,24(4):
113.王伟,陈义勇,谢岩黎,等.2006.草莓红色素的提取工艺研究.常熟理工学院学报,20(4):81-84
114.王霞,高云.2004.黑甜玉米中黑色素提取及纯化工艺研究.食品科学,25(11):198-200
115.王贤纯.2000.姜黄色素及其提制方法.生物学杂志,17(1):36-37.
116.王晓波,刘殿武,王本华,等.2003.马齿苋对高脂动物血脂及脂质过氧化作用的干预实验研究.河北医科大学学报,24(5):261-263
117.王彦吉,宁增高主编.1995.光谱和色谱分析.北京:人民大学出版社.146-148
118.王璋.1999.食品化学.北京:化学工业出版社,568-570
119.王振宇,杨谦.2003.大花葵红色素理化性质的研究.林产化学与工业,23(3):102-104
120.王振宇,杨谦.2005.微生物破壁法提取大花葵花色苷.东北林业大学学报,33(1):56-57
121.王志祥.1994.生化分离技术-制备型高效液相色谱.现代化工,9:18-20
122.王忠合,朱俊晨,陈惠音.2007.双层原花青素微胶囊的试验研究.食品科学,28(1):102-106
123.魏莉.2005.制备型高效液相色谱技术的研究进展.广东化工,11:5-7
124.吴朝霞,孟宪军,林琳.2005.几种微胶囊包裹方法对葡萄籽原花青素包裹效果的对比研究.食品工业科技,26(9):67-72
125.吴春,陈林林,李健.2006.菟丝子黄酮的微胶囊化及释放性能研究.农业工程学报,22(9):264-266
126.吴东生,金磊.1997.食品工业中的喷雾干燥法微胶囊.食品工业科技,17(1):78-82
127.吴恩岐.2002.花瓣花色素的定性分析.内蒙古师范大学学报,31(1):64-67
128.吴满平.1988.载脂蛋白基因和功能研究.生理科学进展,19(2):135
129.吴文林,胡天喜.1997.几种黄酮类化合物对羟自由基引起的DNA损伤的保护作用.自由基生命科学进展,(5):101-104
130.吴信子,朴京一,张小勇,等.2001.蓝靛果花青索的分离与鉴定.延边大学学报,27(3):191-194
131.肖道安,李秋红,陈芳.2007.杜仲叶提取物微胶囊化研究.赣南医学院学报,27(3):321-323
132.谢棒祥,张敏红.2003.生物类黄酮的生理功能极其应用研究进展.动物营养学报,15(2):11-15
133.谢凤霞,邱祖民,涂盛辉,等.2005.浸提法与超声波法提取法提取栀子黄色素的比较.南昌大学学报(理科版),29(3):278-281
134.熊何健,马玉婵,吴国宏.2005.茶多酚的提取及微胶囊化工艺研究.食品科学,26(10):137-140.
135.徐建国,田呈瑞,胡青平.2005.超声波提取桑葚红色素的工艺研究.山西农业大学学报,25(4):380-382
136.徐金瑞,张名位,刘兴华,等.2005.黑大豆种皮花色苷的提取及其抗氧化作用研究.农业工程学报,21(8):161-164
137.徐金瑞,张名位,刘兴华,等.2006.大孔树脂对黑大豆种皮花色苷的纯化研究.农业机械学报,37(4):145-148
138.徐俐,金毅,王斌,等.2006.树莓红色素的提取及稳定性的研究.食品科学,27(5):191-194
139.徐璐,郑建仙.2005.欧洲越桔花色苷的研究概况.中国食品添加剂,(4):43-46
140.徐清海,明霞.2005.天然色素的提取及其生理功能研究.应用化工,34(5):268-270
141.徐文秀,吴彩娥,李强,等.2006.丁香油喷雾干燥微胶囊技术研究.食品科学,27(1):278-281
142.徐渊金,杜琪珍.2006.花色苷分离鉴定方法及其生物活性.食品与发酵工业,32(3):67-72
143.徐忠,张亚丽.2002.D101大孔树脂对萝卜红色素的吸附特性研究.食品科学,(1):59-60
144.许申鸿.2000.天竺葵花抗氧化作用的研究.中国药学杂志,35(4):234-236
145.薛伟明,张效林.1999.红花黄色素的酶法提取应用研究.化学工程,27(1):42-44
146.闫巧娟,韩鲁佳,江正强.纤维素酶法提取芪多糖.中草药,2005,36(12):1804-1807
147.阎师杰,吴彩娥,寇晓虹,等.2003.核桃油微胶囊化工艺的研究.农业工程学报,19(1):168-171
148.杨桂霞.2004.笃斯越橘花色素的分离鉴定及栽培品种花色素的定量分析.吉林农业大学硕士论文.11-26
149.杨桂霞,范海林,李亚东,等.2005.RP--HPLC法测定栽培种越橘果中花色素苷的含量.药物分析杂志,25(10):1222-1224
150.杨小兰,毛立新,张晓云.2005.黑桑椹对高脂血症大鼠的降脂作用研究.食品科学,26(9),509-510
151.杨政水,罗显华,黄静,等.2005.商陆色素的稳定性研究.食品科学,26(9):104-107
152.杨转琴,王磊,范娜,等.2006.柚皮黄酮化合物含量及抗氧化活性的研究.食品科学,27(4):102-105
153.姚伙旺,曾秋平,劳永民,等.2006.食品添加剂对荔枝果皮花色素苷稳定性的影响.食品科学,27(5):152-156
154.叶兴乾,陈健初,苏平.1994.荸荠种杨梅的花色苷组分鉴定.浙江农业大学学报,20(2):188-190
155.于守洋,崔洪斌.2001.中国保健食品的进展,北京:人民卫生出版社,87-89
156.于晓南,张启翔.2002.观赏植物的花色素苷与花色.林业科学,38(3):147-153
157.于泽源,徐雅琴,李兴国.2004.草原樱桃红色素稳定性的研究.食品科学,25(6):111-113
158.余华.2004.玫瑰茄红色素的结构定性研究.食品科技,28(7):55-56
159.张尔贤,俞丽君,周意林,等.1996.Fe~(2+)诱发脂蛋白PUFA过氧化体系及对若干天然产物抗氧化作用的评价.生物化学与生物物理学报,28(2):218-292
160.张捷莉,马天芳,李铁纯,等.2005.板栗皮棕色素的提取与稳定性研究.食品科学,26(9):281-284
161.张峻,吉伟之,陈晓云,等.2002.吸附析法制备低聚原花青素.天然产物研究与开发,14(4):31-33
162.张峻,吉伟之,陈晓云,等.2004.壳聚糖-海藻酸钠微胶囊对葡萄多酚控制释放的研究.食品科学,25(5):102-104
163.张立娟,于国平,周国华.2005.黑木耳多糖酶法提取条件的研究.食品研究与开发,26(3):89-91
164.张连富,杜彦山,牟德华.2006.原花青素的微胶囊化研究.食品与发酵工业,32(2):64-66
165.张名位,郭宝江,张瑞芬,等.2006a.黑米抗氧化活性成分的分离纯化和结构鉴定.中国农业科学,39(1):153-160
166.张名位,张瑞芬,郭宝江,等.2006b.黑米皮提取物的抗氧化与降血脂作用及其成分分析.中国农业科学,39(11):2368-2373
167.张沙艳,闫玉杰.1999.高粱壳中色素提取及稳定性的研究.辽宁师专学报,(1):88-90
168.张世东,王建建,王霞,等.2007.玉米须单体成分纯化及类别鉴定.福建分析测试,16(3):67-69
169.张树宝.2006.超临界CO_2萃取大花葵花色苷工艺研究.特产研究,(1):29-33
170.张惟杰主编.1999.糖复合物生化研究技术.浙江大学出版社:102-105.
171.张燕,谢玫珍,廖小军.2005.热和紫外辐射对红莓花色苷稳定性的影响.食品与发酵工业,31(3):37-40
172.张跃超.2001.果树新品-美国蓝莓.农业科技通讯,(1):16
173.张钟,宫坤,陈守江.2004.黑糯玉米色素的提取及性质研究.中国粮油学报,19(2):62-71
174.张钟,张志海.2006.影响黑糯玉米芯色素抗氧化活性的因素及与其他抗氧化剂效应的比较.食品与发酵工业,30一33
175.赵昶灵,郭维明,陈俊愉.2004.梅花“南京红”花色色素花色苷的分子结构.云南植物研究,26(5):549-557
176.赵晓燕,冯作山,陈复生.2005.番茄红素微胶囊的稳定性研究.食品与发酵工业,31(10):45-47.
177.赵宜江,姚建民.1997.无机膜提取栀子黄色素的工艺研究.南京化工大学学报,19(1):77-81
178.郑光耀.葡萄籽原花青素提取物的生理活性、药用作用及其应用.林产化工通讯,2000,34(1):28-33
179.郑穗平,沈继红,郭勇.1997.利用玫瑰茄细胞悬浮培养技术生产花色苷的初步研究.食品工业科 技,(6):29-31
180.钟丽玉.黑米分子结构解析.无锡轻工大学学报,1996,15(1):33-38
181.周宇,闫国华,张开春,等.2006.桔梗花色苷的提取与稳定性研究.食品工业科技,27(3):162-165
182.朱蓓微,金英实.2003.提高越桔天然色素稳定性方法的研究.食品科学,24(5):81-84
183.朱洪梅,韩永斌,顾振新,等.2006a.大孔树脂对甘薯色素的吸附与解吸特性研究.农业工程学报,22(5):153-156
184.朱洪梅,韩永斌,顾振新,等.2006b.单宁对紫甘薯花色苷的辅色作用的研究.南京农业大学学报,29(3):98-102
185.朱文学,吴龙奇,易军鹏,等.2006.牡丹花色素理化性质的研究.农业工程学报,22(6):224-226
186.朱选,阳会军,黄慧敏,等.2000.β-胡萝卜素微胶囊化工艺参数的研究.食品与机械,(5):11-13
187.朱英,罗永明,付小梅.1989.大孔树脂分离油茶皂甙和黄酮的研究.林产化学与工业,19(1):40-42
188.Aaby K,Skrede G,and Wrolstad R E.2005.Phenolic composition and antioxidant activities in flesh and achenes of strawberries(Fragaria ananassa).J.Agric.Food Chem.,53(10):4032-4040
189.Annamaryin D S.1997.Antioxidant ability of anthocyanins against ascorbic acid oxidation,Phytochemistry.45(4):671-674
190.Armstrong M L.1976.Evidence of regression of atherosclerosis in primates and man.Postgrad.Med.52,456
191.Assmann G,Gotto A M.2004.HDL cholesterol and protective factors in atherosclerosis.Circulation,109:Ⅲ8-14
192.Bagchi D,Sen chandan K,Ray S D,et al.2003.Molecular mechanisms of cardioprotection by a novel grape seed proantocyanidin extract.Mutation Research.523-524.
193.Bao J S,Cai Y Z,Sun M,et al.2005.Anthocyanins,Flavonols,and free rdical scavenging activity of Chinese bayberry(Myrica rubra) extracts and their colour properties and stability.J.Agric.Food Chem.,53(6):2327-2332
194.Bate-Smith E C.1948.Paper chromatography of anthocyanins and related substances in petal extracts.Nature,(4100):835-838
195.Brown M S,Faust J R,and Goldstein J L.1975.Role of the low density lipoprotein receptor in regulating the contents of free and esterified cholesterol in human fibroblasts.J.Clin.Invest.55,783
196.Cao G,Sofic E,Prior R L.1997.Antioxidant and prooxidant behavior of flavoniods:structure-activity relation.Free Redical Biology and Medicine.22(5):746-760
197.Cao L.1994.Quantification and distribution of simple and acylated anthocyanins and other phenolics in blueberries.Food science,59:1057-1059.
198.Castro L,and Freeman B.2001.Reactive oxygen species in human health and disease.Nutrition.17:161-165
199.Chandra A,Rana J,Li Y Q.2001.Separation,idertification,quantification,and method validation of anthocyanins in botanical supplement raw materials by HPLC and HPLC-MS.J.Agric.Food Chem.,49(8):3515-3521
200.Charron C S,Clevidence B A,Britz S J,et al.2007.Effect of dose size on bilavailability of acylated and nonacylated anthocyanins from red cabbage(Brassica oleracea L.Var.capitata).J.Agric.Food Chem.,55(13):5354-5362
201.Chen L J,Hrazdina G.1981.Structural aspects of anthocyanin flavonoid complex formation and its role in plant color.Phythchem.,20:297-303
202.Chou E J.2001.Effect of ingestion of purple grae juice on endothelial function in patieuts with coronary heart disease.The American Journal of Cardiology,88(9):553-555
203.Connor A.M.,et al.2002.Coenotypic and environmental variation in antioxidant activity,totalphenolic content and anthocyanin content among blueberry cultivars,Amer.Soc.Hort.Sci,127(1):89-97.
204.Cooke D N,Thomasset S,Boocock D J,et al.2006.Development of analyses by high-performance liquid chromatography and liquid chromatography/tandem mass spectrometry of bilberry(Vaccinium myrtilus) anthocyanins in human plama and urine.J.Agric.Food Chem.,54(19):7009-7013
205.Dangles O,Saito N,Brouillard R.1993.Anthocyanin intramolecular copigment effect.Phytochemistry.34:119-124
206.Degenhardt A,Knapp H,Winterhalter P.2000.Separation and purification of anthocyanins by high-speed countercurrent chromatography and screening for antioxidant ctivity.J.Agric.Food Chem.,48(2):338-343
207.Di Paola-Naranjo R D,Sanchez-Sanchez J,Gonzalez-Paramas A M,et al.2004.Liquid chromatographic-mass spectrometric analysis of anthocyanin composition of dark blue bee pollen from Echium plantagineum.J Chromatogr A.,1054(1-2):205-210
208.Duh P D,YenG C.1997.Antixidant activities of three herbal water extracts.Food Chem,60:639-645
209.Ghiselli A,Nardini M,Baldi A.,et al.1998.Antioxidant activity of different phenolic fractions separated from An Italian red wine,J.Agric.Food Chem.,46:361-367
210.Hanson C.1999.The advantage of natural colors in food products.Food Marketing and Technology,99:12-15
211.Harborne J B.1958a.Spectral methods of characterizing anthoeyanins.J.Bio.Chem,70:22
212.Harborne J B.1958b.The chromatographic identification of anthoeyanin pigments.Journal of Chromatography,1:473-488
213.He J,Magnuson B A,and Giusti M M.2005.Analysis of anthoeyanins in rats intestinal contents-impact of anthocyanins chemical structure on fecal excretion.J.Agric.Food Chem.,53(8):2859-2866
214.Heinonen I M,Meyer A S,and Frankel E N.1998.Antioxidant activity of berry phenolics on human low density lipoprotein and liposome oxidation.J.Agrie.Food Chem.,46:4107-4112
215.Hennink W E,Nostrum C F.2002.Novel cross-linking methods to design hydrogels.Advanced Drug Delivery Reviews,54:13-36
216.Heo H J and Lee C Y.2005.Strawberry and its anthoeyanins reduces oxidative stress-induced apoptosis in PC12 cells.J.Agric.Food Chem.,53(6):1984-1989
217.Iehiyanagi T,Shida Y,Rahman M M,et al.2006.Bioavailability and tissue distribution of anthoeyanins in bilberry(Vaccinium myrtillus L.) extract in rats.J.Agrie.Food Chem.,54(18):6578-6587
218.Iehiyangi T,Hatano Y,Matsugo S,et al.2004.Structural dependence of HPLC separation pattern of anthocyanins from Bilberry(Vaceinium myrtillus L.).Chem Pharm Bull(Tokyo),52(5):628-630
219.Igarashi K,Kimura Y,Takenaka A.2000.Preventive effects of detary cabbage acylated ant-hocyanins on paraqual-induced oxidative stress in rats.Biosci,Biotechnol,Biochem.64:1600-1607
220.Joseph A.B.1973.Microencapsulation of foods and related products.Food technology,(11):34-44
221.Jung C H,Seog H M,Choi I W,et al.2005.Antioxiant activities of cultivated and wild Korean ginseng leaves.Food Chemistry,92:535-540
222.Kalt W and Mcdonald J E.1996.Chemical composition of lowbush blueberry cultivars.J.Amer.Soc.Hort.Sci.;121(1):142-146.
223.Kalt W,Forney C F,Martin A,et al.1999.Antioxidant,capacity,vitarninC,phenolics,and anthocyanins after fresh storage of small fruits.J.Agric.Food chem.,47,4638-4644.
224.Kalt W,Blumber J B,McDonald J E,et al.2008.Identification of anthocyanins in the liver,eye,and brain of blueberry-fed pigs.J Agric Food Chem.,56(3):705-712.
225.Katsube N,Iwashita K,Tsushida T,et al.2003.Induction of apoptosis in cancer cells by bilberry(Vaccinium myrtillus) and the anthocyanins.J.Agric.Food Chem.,51(1):68-752
26.Kayamori F,Igarashi K.1994.Effects of dietary nasunio on the serum cholesterol level in rats.Biosci.Biotechnol.Biochem.58:507-571.
227.Kim M J,Hyun J N,Kim J A,et al.2007.Relationship between phenolic compounds,anthocyanins content and antioxidant activity in colored barley germplasm.J.Agric.Food Chem.55(12):4802-4809
228.Lapidot T,Hurel S,Akiri B,et al.1999.pH-dependent forms of red wine anthocyanins as antioxidants.J.Agric.Food Chem.47:67-70
229.Lapidot T,Harel S,Granit R,et al.1998.Bioavailability of red wine anthocyanins as detected in human urine.J.A gric.Food Chem.,46910):4297-4302.
230.Laplaud P M,Lelubre A,and Chapman M J.1997.Antioxidant of total monomeric anthocyanin pigment content of fruits juices,beverages,natural colorants,and wines by the pH different method:collaborative study.J AOAC Int,88(5):1269-1278
231.Lau F C,Shukitt-Hale B and Joseph J A.2005.The beneficial effects of fruit polyphenols on brain aging.Neurobiology of aging,26(1):128-132
232.Lee J and Wrolstad R E.2004.Extraction of anthocyanins and polyphenolics from blueberry processing waste.Journal of food science,69(7):564-573
233.Lee L,Durst R W,Wrolatad R E.2005.Determination of total monomeric anthocyanin pigment content of fruit juices,beverages,natural colorants,and wines by the pH differental method:collaborative study.J AOAC int,88(5):1269-1278
234.Liu X D,Takuroh Atarashi,Takeshi Furuta,et al.2001.Microencapsulation of emulsified hydrophobic flavors by spray drying.Drying Technology.19(7):1361-1374
235.Ma Y H,Li W L,Peng Y F,et al.2003.The extractive technique of red pigment of Bougainvillea spectabilis wild.Food Sciences,24(1):78-80
236.Matsumoto H,Hanamra S,Kawakami T et al.2001.Preparative-scale isolation of four anthocyanin components of black current(Ribes nigrum L.)fruits.J.Agric.Food Chem.,49(3):1541-1545
237.Maurizio F.1995.Preparative high-performance liquid chromatography for the purification of natural anthocyanins.Jourhal of Chromatography A,(692):213-219
238.Mazza G,Cacace J E,Kay C D.2004.Methods of analysis for anthocyanins in plants and biological fluits.J AOAC int,87(1):129-145
239.Mazza G,Kay C D,Cottrell T,et al.2002.Absorption of anthocyanins from blueberries and serum antioxidant status in human subjects.J.Agric.Food Chem.,50(26):7731-7737
240.Mazza G.1987.Recent Development in the stabilization of anthocyanins in food products.Food Chemistry,25:207-225
241.McDougall G J,Dobson P,Smith P,et al.2005.Assessing potential bioavability of raspberry anthocyanins using an in vitro digestion system.J.Agric.Food Chem.,53(10):5896-5904
242.McGhie T K,Ainge G D,Barnett L E,et al.2003.Anthocyanins glycosides from berry fruits are absorbed and excreted unmetabolized by both humans and rats.J.Agric.Food Chem.,51(16):4539-4548
243.McGhie T K,Rowan D R,Edwards P J.2006.Structural identification of two major anthocyanins components of boysenberry by NMR spectroscopy J.Agric.Food Chem.,54(23):8756-8761
244.Milbury P E,Cao G H,Prior R L.2002,Bioavailablility of elderberry anthocyanins.Mechanisms of Ageing and Development,123:997-1006
245.Motoori S.1998The Food Industry,41(16):22-28
246.Moyer R A,Hummer K E,Finn C E,Wrolstad R E.2002.Anthocyanins,phenolics,and antioxidant capacity in diverse small fruits:Vaccinium,Rubis,and Ribes.J.Agric.FoodChem.,50:519-525
247.Nakano T,Nakano K,Sim J S.1998.Extraction of glycosaminoglycan peptide from bovine nasal cartilage with 0.1M sodium acetate.J.Agric Food Chem,(46):772-778.
248.Nielsen I LF,Dragsted L O,Ravn-Haren G,et al.2003.Absorption and excretion of black currant anthocyanins in humans and watanabe heritable hypedipidemie rabbits.J.Agric.Food Chem.,51(9):2813-2820
249.Norio Sito,Kenjiro Toki,Hidekazu Moriyama.2002.Acylated anthocyanins from the blue-violet flowers of anemone coronaria.Phytochemistry.60:365-373
250.Nyman N A and Kumpulainen J T.determination of anthocyanins in berries and red wine by High-performance liquid chromatography.J.Agric.Food Chem.,2001,49(9):4183-4187
251.Obedeg L W.1986.Free radicals aging and degenerative disease.Alan R Liss Inc,42:301-311
252.Ock-Sook Yi.1997.Antioxidant activity of grape extracts in a lecthin liposome system.JAOCS,74(10):1301-1306
253.Olsson M E,Gustawsson K E,Andersson S,et al.2004.Inhibition of cancer cell proliferation in Vitro by fruit and berry extracts and correlation with antioxidant levels.J.Agric.Food Chem.,52(24):7264-7271
254.Osawa Y.Anthocyanins as food colors.New York:academic Press,1982,85
255.Otukia T,Matsufujia H,Takedaa M.2002.Acylated anthocyanins from red radish.Phytochemistry.60:79-87
256.Pauleti M S,Mestoy P.1999.Butter microencapsulation as affected by composition of wall material and fat.J.Food Sci.64(2):279
257.Pinelo M,Rubilar M,Jerez M,et al.2005.Effect of solvent,temperature,and solvent-to-solid ratio on the total phenolic content and antiradical activity of extracts from different components of grape pomace.J.Agric.Food Chem.,53(6):2111-2117
258.Prior R L,Lazarus S A,Cao G H,et al.2001.Identification of procyanidins and anthocynins in blueberries and cranberries(Vaccinium SPP.) using high-performance liquid chromatography/Mass spectrometry.J.Agric.Food Chem.,49(3):1270-1276
259.Prior R L,Wu X,Gu L,et al.2008.Whole berries versus berry anthocyanins:interactions with dietary fat levels in the C57BL/6J mouse model of obesity.J Agric Food Chem.,56(3):647-653.
260.Quan Q z,Dang R Y,Li T S.2001.Adsorbing and separating radish red pigment by X-5 Resin.Acta Botanica Boreal-Occidentalia Sinica,21(6):1218-1222.
261.Renand S,Zorgelil M.1992.Wine,alcohol,platelets and the French paradox for coronary heart disease.Lancet.33(9):1523-1526
262.Saito N,Tatsuzawa F,Yoda K,et al.1995.Acylated cyaniding glycosides in the viole t-blue flowers of ipomoea purpurea.Phytochemistry.40(4):1283-12 8 9
263.Satue Gracia M T,Heinonen M,Frankel E N.1997.Anthocyanins as antioxidants on human low-density lipoprotein and lecithin-liposome systems.J.Agric Food Chem,45(3):362-367.
264.Schwarz M,Hillebrand S,Habben S,Degenhardt A,et al.2003.Application of high-speed counter-current chromatography to the large-scale isolation of anthocyanins.Biochem Eng J,14(3):179-189
265.Seeram N P,Adams L S,Zhang Y J et al.2006.Blackberry black raspberry,blueberry,cranberry,red raspberry,and strawberry extracts inhibit growth and stimulate apoptosis of human cancer cells in vitro.J.Agric.Food Chem.,54(25):9329-9339
266.Shahidi F,Han X Q.1993.Encapsulation of food ingredients.Critical Review Food Sci.Nutri.33(6):501.
267.Smironff N,Cumbes Q J.1989.Hyroxyl radical scavenging activity of compatible solutes.Phytochemistry,28(4):1051-1560
268.Solomon A,Golubowicz S,Yablowicz Z,et al.2006.Antioxidant activities and anthocyanin content of fresh fruits of common Fig(Ficus carica L.).J.Agric.Food Chem.,54(20):7717-7723
269.Suda I,Oki T,Masuda M,et al.2002,Direct absorption of acylated anthocyanin in purple- fleshed sweet potato into rats.J.Agric.Food Chem.,50(6):1672-1676
270.Talavera S,Felgines C,Texier O,et al.2005.Anthocyanin metabolism in rats and their distribution to digestive area,Kidney,and brain.J.Agric.Food Chem.,53(10):3902-3908
271.Tan BKH.1991.Sixth SEA-Western Pacific Regional Meeting of Pharmacologists.Hong Kong:234.
272.Terasawa N,Saotome A,Tachimura Y,et al.2007.Identification and some properties of anthocyanin isolated from zuiki,Stalk of colocasia esculenta.J.Agric.Food Chem.55(10):4154-4159
273.Tian Q G,Giusti M M,Stoner G D,et al.2005.Schwartz.Screening for anthocyanins using high-performance liquid chromatography coupled to electrospray ionization tandem mass.Journal of Chromatography A,109(1-2):72-82
274.Tian Q G,Giusti M M,Stoner G D,et al.2006.Urinart excretion of black raspberry(Rubus occidentalis) anthocyanins and their metabolites.J.Agric.Food Chem.,54(4):1467-1472
275.Tomas J P,Geiger PG,Girotti AM.1993.Lethal Damage to endothelial cell by oxidized low density lipoprotein:role of selenoperoxidase in cyctoprotection agains lipid hydroperoperoxide-and iron-mediated reactions.Lipid Res,479-490
276.Tsuda T.1994.Antioxidntive Activity of Anthoocyanin Pigments Cyanidin 3- O-β-D-gluceside and Cyandin,J.Agre.Food Chem.42(11):2407-2410
277.Tsuda T,Horio F,Osawa T.1999.Absorption and metabolism of cyanidin3-O-β-D-gl ucoside in rats.FEBS Letters,449:179-I82
278.Tsuda T,Watanade M,Ohshima K,et al.1994.Antioxidative activity of the anthocyanin pigments cyanidin3-O-β-D-Glucoside and cyaniding.J Agric.Food Chem,42(10):2407-2410
279.Vander Meer R,De Vrises H T,West C E,et al.Casein-induced hypercholesterolaemia in rabbits is calcium-dependent.Atherosclerosis,1985,56:139-147
280.Vinson J A.2001.Red wine,dealcoholized red wine and especially grape juice inhibit atherosclerusis in a hamster mode.Atherosclerosis.156:67-72
281.Voyiaziks E,Glodberg I J,Plump A S,et al.PapoA triglyceridemia and increased atherosclerosis in human apoB transgenic mice.J lipid Res1998,39(2):313-321
282.Wang H,Cao G H,and Prior R L.1997.Oxygen radical absorbing capacity of anthocyanins.J.Agric.Food Chem.,45,304-309.
283.Wang H,Liu G,Yang L,et al.2005.Effects of Liensinine on blood lipids and lipid peroxidation in experimental hyperlipidemia rats.Natural Product Research and Development.17(6):722-725.
284.Wang J and Mazza G.2002.Effects of anthocyanins and other phenolic compounds on the production of tumor necrosis factor α in LPS/IFN-γ-activated RAW 264.7 macrophages.J.Agric.Food Chem,50(15):4183-4189
285.Wang Y,Chen F C,Jenny C,et al.2005.Dietary supplementation with blueberries,spinach,or spirulina reduces ischemic brain damage.Experimental Neurology, 193(1):75-84
286.Wilson T, Porcari J P, Harbin D.1998.Cranberry extract inhibits low density lipoprotein oxidantion.Life Science, 62 (24):381-386
287.Wu Q K., Koponen J M, Mykkanen H M, et al.2007.Berry phenolic extracts modulate the expression of p21~(WAFI) and bax but bcl-2 in HT-29 colon cancer cells.J.Agric.Food Chem.55(4):1156-1163
288.Wu X L and Prior RL.2005.Systematic identification and characterization of anthocyanins by HPLC-ESI-MS/MS in common foods in the United States: Fruits and berries.J.Agric.Food Chem., 53(7):2589-2599
289.Wu X L, Beecher G R, Holden J M, et al.2006.Concentrations of anthocyanins in common foods in the Uniteds States and estimation of normal consumption.J.Agric.Food Chem., 54(11):4069-4075
290.Yen G C , Chen H Y.1995.Antioxidant activity of various tea extracts in reation to their antimutagenicity.J Agric Food Chem, 43(1):27-32
291.Yi W G, Akoh C C, Fischer J ,et al.2006a.Effects of phenolic compounds in blueberries and muscadine grapes on HepG2 cell viability and apoptosis.Food Research International, 39(5):628-638
292.Yi W G , Akoh C C, Fischer J, et al.2006b.Absorption of anthocyanins from blueberry extracts by caco-2 human intestinal cell monolyers.J.Agric.Food Chem., 54(15):5651-5658
293.Yi W G , Fischer J, Krewer G, et al.2005 .Phenolic compounds from blueberries can inhibit colon cancer cell proliferation and induce apoptosis.J.Agric.Food Chem., 53(18):7320-7329
294.Youdim K.A, Mcdonald J, Kalt W, et al.2002.Potential role of dietary flavonoids in reducing microvascular endothelium vulnerability to oxidative and inflammatory insults.the Journal of Nutritionall Biochemistry, 13(5):282-288
295.Zanatta C F.Cuevas E, Bobbio F O, et al.2005.Determination of anthocyanins from cmu-camu (Myrciaria dubia) by HPLC-PDA.HPLC-MS, and NMR.53(24):9531-9535
296.Zhang Z, Kou X L, Fugal K, et al.2004.Comparison of HPLC methods for determination of anthocyanins and anthocyaninidins in bilberry extracts.J.Agric.Food Chem., 52(4):688-691
297.Zhao C W, Giusti MM, Malik M,et al.2004.Effects of commercial anthocyanin-rich extracts on colonic cancer and nontumorigenic colonic cell growth.J.Agric.Food Chem., 52(20):6122-6128
298.Zheng W and Wang S Y.2003.Oxygen radical absorbing cpacity of phenolics in blueberries,cranberries,chokeberries, and lingonberries.J.Agric.Food Chem., 51(2):502-509
299.Zhu L S, Ma X C, Liu,et al.2004.Effects of total flavones of buckwheat leaf on blood lipid and lipid peroxides.Chines Journal rehabilitation, 8(24):5178-5179
2.毕红霞,李建伟,陈玮,等.AB-8吸附树脂对欧李色素的吸附和精制.郑州工程学院学报,2004,25(2):40-42
3.蔡俊.2005.酶在番茄红素提取过程中的应用研究.湖北工业大学学报,20(1):8-10
4.曹炜,尉亚辉,郭斌.2002.用化学发光法研究蜂胶对氧自由基的清除作用,光子学报,31(2):162-164
5.陈炳华,刘剑秋.2003.不同溶剂和保存条件对尾叶悬钩子花色素苷提取及稳定性的影响.植物资源与环境学报,12(4):11-15
6.陈丛瑾,黄克瀛,张东峰,等.2006.超声波提取白英果红色素的工艺研究.食品研究与开发,27(8):80-83
7.陈建初,叶兴乾.2005.抗坏血酸对杨梅花色苷色素稳定性的影响.浙江大学学报(农业与生命科学版),31(3):298-300
8.陈连文,刘敬兰,师建华.2001.黑糯玉米色素的提取及稳定性研究.食品科技,27(1):39-41
9.陈留勇,孟宪军,贾薇,等.2004.黄桃水溶性多糖的抗肿瘤作用及清除自由基、提高免疫活性研究食品科学,25(2):167-170
10.陈伟,孟宪军,王立男.2006.玫瑰花红色素提取工艺研究.食品研究与开发,27(3):50-52
11.陈晓辉,谭晓杰,田中克佳.2004.制备型高效液相色谱在中药野菊花化学成分分离中的应用.药品评价,1(5):359-361
12.陈勇,张晴.2001.AB-8大孔吸附树脂吸附和分离紫甘薯色素的研究.中国食品添加剂,(1):6-9
13.陈勇,赵春芬,孙清,等.1998.紫苏色素稳定性的初步研究.中国食品添加剂,(3):4-8
14.陈志华,李静.2000.粉状脂溶性茶多酚在色拉油中的性能实验.中国油脂,25:55-57
15.程超,莫开菊,汪兴平.2005.天然水溶性火棘色素在不同环境条件下的稳定性研究.食品科学,26(3):72-74
16.程瑛琨,李蕾,王丹梅,等.2006.酸浆宿萼色素的提取及稳定性的研究.食品工业科技,27(12):58-64
17.程元恺.1993.脂质过氧化与抗氧化酶.工业卫生与职业病,13(4):254
18.崔僖山编绎.1992.食品文摘,2:9-11
19.党蕊叶,权清转.2005.大孔吸附树脂纯化矮牵牛化红色素.西北农业学报,14(3):145-147,158
20.段翰英,李远志,黄苇,等.2004.微胶囊Vc加工方法及性质研究.食品工业科技,25(8):107-108
21.樊金玲,丁霄霖.2006.沙棘籽提取物抗氧化活性与酚类组成的研究.天然产物研究与开发,18(4):529-524,554
22.范润珍,韩少伟,林宏图.2006.山竹壳色素的提取及其稳定性研究.食品科学,27(8):358-362
23.方允中,郑荣梁主编.2002.自由基生物学的理论与应用.北京:科学出版社,60-69
24.冯卫华,刘邻渭,许克勇.2004.猕猴桃籽油微胶囊化技术研究.农业工程学报,20(1):234-237
25.葛毅强.2000.微胶囊型天然维生素E粉末的研制.食品工业,(3):32-34
26.顾红梅,张新申,蒋小平.2004.超声波法和冻结-融解法相结合提取紫薯中花色甙.食品科学,25(7):104-107
27.顾文秀,谢为明,夏文水.2005.超声波提取乌饭树叶色素及其稳定性的研究.林产化学与工 业,25(4):74-78
28.郭红,陈在嘉,徐义枢.1991.血清脂质与脂质过氧化关系的临床研究.中国循环杂志,6(5):373-375
29.郭颖.2001.微胶囊化左旋肉碱.江南大学硕士论文,10
30.郝建军,刘延吉.2001.植物生理实验技术.沈阳:辽宁科学技术出版社.
31.何耀,李良涛,万志恒.冠心病血清载脂蛋白A1和B100的观察.中华心血管病杂志,1990,18(4):217-219
32.胡天喜.1997.自由基生命科学进展(第5集).北京:原子能出版社,66-67
33.黄涛,卢起来.1997.月季花红色素的提取与性质研究.精细化学,14(5):21-23
34.黄延春,索全伶,贺文智,等.2005.天然实用红木色素的分离纯化与结构表征.天然产物研究与开发,17(6):681-684
35.回瑞华,侯冬严.1995.近代有机仪器分析.吉林:吉林大学出版社
36.霍琳琳,苏平,吕英华.2005.分光光度法测定桑葚总花色苷含量的研究.酿酒,23(4):88-89
37.姜平平,吕晓玲,姚秀玲,等.2002.紫心甘薯花色苷抗氧化活性体外实验研究.中国食品添加剂,(6):8-11
38.姜平平,吕晓玲朱惠丽.2003.花色苷类物质分离鉴定方法.中国食品添加剂,(4):108-111
39.焦中高,刘杰超,王思新.2003.黑莓红色素抗氧化活性的研究.食品科技,(8):63-65
40.金呈之.2006.紫草色素制备的研究进展.江苏食品发酵,(2):16-20
41.金锋,李新华,张森.2006.玉米色素微胶囊制备工艺研究.中国食品添加剂,48-52
42.金杰,李志西,张锋,等.2006.桑椹醋提取物对二苯代苦味酰基自由基(DPPH·)的清除作用.西北农林科技大学学报(自然科学版),35(3):135-137
43.金宁,刘通迅.2007.山楂原花青素的抗氧化活性.食品与发酵工业,33(1):45-47
44.李春阳.2006.葡萄籽中原花青素的提取纯化及其结构和功能研究.江南大学博士论文.
45.李大婧,刘春泉,方桂珍.2007.酶为介质提取万寿菊花中叶黄素的工艺研究.农业工程学报,23(5):232-236
46.李剑君,李稳宏.2001.葛根总黄酮中葛根素的分离研究.西北大学学报(自然科学版),34(4):311-314
47.李进,彭宇,彭子模.2004.鸡冠花红色素理化性质及其稳定性研究.生物技术,14(1):21-24
48.李进.2005.大孔树脂吸附分离黑果枸杞色素的研究.食品科学,26(6):47-51
49.李军,徐本明,刘珂.2004.曲克卢丁对照品的制备液相色谱分离.中国医药工业杂志,35:285-287
50.李善吉,伍胜君.2005.大孔树脂的应用研究概况.广东轻工职业技术学院学报,4(2):11-13
51.李书国,薛文通,李雪梅,等.2005.乙基纤维素微胶囊化Vc工艺参数及其活性保护的研究.食品工业科技,26(5):143-148
52.李亚东等.2001.中国蓝莓产业化发展的前景.沈阳农业大学学报,3(1):39-42
53.李亚东.2002.蓝莓果实的成分及保健机能.中国食品与营养,(1):27-285
4.李永强,张玉娜,王明珍,等.2005.白刺果实色素的提取及理化性质研究.山东农业大学学报(自然科学版),36(1):75-81
55.励建荣,岑沛霖,Joyce D C.2002.杨梅汁内花色苷热降解动力学研究.科技通报,18(1):1-5
56.梁少玲,蔡宇,杨燕霞,等.2006.大孔树脂吸附在黄酮及皂苷类成分分离纯化中的应用.时珍国医国 药,17(2):277-278
57.梁治齐.1999.微胶囊技术及其应用.中国轻工业出版社
58.林秋风,王凌云,李药兰,等.2003.AB-8大孔树脂分离长瓣金莲花总黄酮.暨南大学学报(自然科学版),24(1):55-58
59.凌关庭.2003a.食品添加剂手册(第三版).北京:化学工业出版社,685-690
60.凌关庭.2003b.可供开发食品添加剂(Ⅸ):兰莓提取物及其抗氧化作用.粮食与油脂,(6):45-48.
61.凌关庭.2004.抗氧化食品与健康.北京:化学工业出版社(第一版),1-58,233-234
62.刘斌,石任兵,余超.2002.影响大孔树脂吸附分离中草药化学成分的因素.中草药,33(5):475-476
63.刘富梁,梁华正,刘成佐,等.大孔树脂分离纯化商陆红色素的研究.化工时刊,2006,20(1):33-35
64.刘广文.2001.喷雾干燥实用技术大全.北京:中国轻工业出版社
65.刘建新,胡浩斌,赵国林.2006.叶子花红色素的提取及其稳定性研究.食品科学,27(8):98-102
66.刘杰超,焦中高,周红平,等.2006.甜樱桃红色素的体外抗氧化活性.果树学报,23(5):756-759
67.刘邻渭.2000.食品化学.北京:中国农业出版社.
68.刘玲,李霞,金同铭.1998.高压液相色谱在花色素苷分析中的应用.北京农业科学,16(2):30-33
69.刘小珍.2002.脐橙色素的稳定性.光谱实验室,19(6):784-785
70.刘云海,刘瑛,曹小红,等.2004.天然食用色素花青素的微胶囊化.食品工业科技,25(12):109-110
71.卢蓉蓉,张国农.2001.喷雾干燥微胶囊技术中的玻璃化转变.中国乳品工业,6:24-27
72.卢钰,董现义,杜景平.2005.花色苷研究进展.山东农业大学学报,35(2):315-320
73.陆国权,蓝小明,楼晓波,等.1996.紫心甘薯红色素理化性质的研究.浙江农业大学学报.22(3):308-301
74.陆国权,吴小蓉.1997.黑豆皮色素的提取及其理化性质研究.中国粮油学报.12(3):53-58
75.吕晓玲,孔德莉,姚秀玲,等.2006.采用荧光化学发光法研究甜菜红色素的抗氧化作用.食品与发酵工业,32(10):70-73.
76.马银海,彭永芳,张永丽,等.2000.AB-8树脂吸附和分离黑糯米黑色素.食品科学,21(12):93-94
77.马银海,彭永芳,付云海,等.2005a.一串红色素的提取工艺研究.食品科学,26(9):311-313
78.马银海,彭永芳,杨丽琳,等.2005b.草莓红色素的提取及树脂吸附.食品科学,26(7):56-58
79.孟红梅,韩多红,李彩霞,等.2006.唐古特白刺果实红色素稳定性研究.食品科学,27(8):72-75
80.孟洁.黑大豆色素的研究.2000.青岛大学学报,13(2):29-33
81.孟志芬,董彩霞,孟现超.2005.酶法提取芝麻叶中总黄酮的工艺研究郑州大学学报(工学版):26(4):53-56
82.莫开菊,柳圣,程超.2006.生姜黄酮的抗氧化活性研究.食品科学,27(9):110-115
83.聂芊,吴春.2002.辅色素对笃柿天然色素稳定性的影响.食品研究与开发,23(3):31-32
84.帕提古丽,马合木提,高莉.2004.酸浆花萼色素的提取及理化性质研究.食品科学.25(9):35-38
85.潘见,陈强,谢慧明,等.1999.大孔树脂对葛根黄酮的吸附分离特性研究.农业工程学报,15(1):236
86.庞战军,周玫,陈瑗.2000.自由基医学研究方法.北京:人民卫生出版社,23-115
87.祁建军,宋经元,雷和田,等.2002.根癌农杆菌转化紫草的研究.云南植物研究,24(5):656-658
88.乔华,张生万,李美萍,等.2006.天然色素稳定性研究及其新的类型划分.食品科学,27(9):69-73
89.钦传光,马丽,丁焰,等.1998.纸色谱法鉴定蛇莓红色素的成分.湖北工学院学报,13(3):5-8
90.邱业先,王桃云.2002.野菊花黄色素提取工艺研究.食品与发酵工业,28(3):33-36
91.任海,查丽杭,秦川,等.2002.大孔树脂分离提取麻黄碱的研究.离子交换与吸附,18(2):97
92.上官新晨,沈勇根,徐明生,等.2006.大孔树脂对紫红薯色素吸附和解吸的特性研究.食品科技,(7):117-120
93.尚庆坤,祝红,阎吉昌.1999.高效制备液相色谱法分离制备虻中的多肽样品.分析化学研究简报,27(8):924-926
94.宋常春,王晓鹏.2003.壳聚糖膜的制备及对植物色素富集作用的研究.食品科学,24(10):88-90
95.苏金为.1999.黑米色素的提取和组成分析.福建农业大学学报,28(2):233-237
96.孙贵宝.2002.越橘的保健作用及各国栽培发展趋势.农机化研究,(3):225
97.孙健,蒋跃明,彭子模.2005.利用大孔吸附树脂提取蜀葵花色素的研究.生物技术,15(1):63-65
98.孙庆杰,顾琼芬.1997.超临界萃取技术在天然色素中的应用.农牧产品开发,(12);3-5
99.孙艳华,苗靖.2006.酶法提取荞麦茎叶中黄酮的研究.食品工业,(3):16-18
100.孙阳昭,孙志奸,廖小军,等.2006.红莓果中花青素的提取工艺研究.食品与发酵工业,32(1):129-133
101.孙志健,张燕,陈芳,等.2005.对蓝莓产业化发展的思考.食品工业科技,26(12):183-184
102.唐传核,马青.1998.花色苷色素以及其在食品中的应用.中国食品用化学品,(6):29-31
103.唐传核.2005.植物生物活性物质.北京:化学工业出版社.247
104.唐璇,李稳红,李新生,等.2006.黄姜中黄色素的提取工艺研究.食品科学,27(10):337-340
105.王冬梅,尉芹,马希汉.2002.大孔树脂在药用植物有效成分分离中的应用,西北林学院学报,17(1):60-63
106.王晖,刘佳佳.2003.银杏黄酮的酶法提取工艺研究.中药材,26(12):887-888
107.王慧琴,谢明勇,付志红.2003.红花红色素的抗氧化活性.无锡轻工大学学报,22(5):98-101
108.王可兴,潘思轶,罗锐.2003.原花青素微胶囊工艺及稳定性研究.食品科学,24(8):88-90.
109.王克勤.1995.脂蛋白与动脉粥样硬化.北京:人民卫生出版社,417
110.王力生,邹节明,郭亚健.2004.D101大孔树脂纯化苦玄参总皂苷的研究.中草药,35(5):515-517
111.王琳芳,杨克恭.2001.医学分子生物学原理.北京:高等教育出版社,956-957
112.王琳琳,凌文华,马静,等.2002.膳食黑米皮抑制高脂诱导家兔动脉粥样硬化形成及其机制研究.营养学报,24(4):
113.王伟,陈义勇,谢岩黎,等.2006.草莓红色素的提取工艺研究.常熟理工学院学报,20(4):81-84
114.王霞,高云.2004.黑甜玉米中黑色素提取及纯化工艺研究.食品科学,25(11):198-200
115.王贤纯.2000.姜黄色素及其提制方法.生物学杂志,17(1):36-37.
116.王晓波,刘殿武,王本华,等.2003.马齿苋对高脂动物血脂及脂质过氧化作用的干预实验研究.河北医科大学学报,24(5):261-263
117.王彦吉,宁增高主编.1995.光谱和色谱分析.北京:人民大学出版社.146-148
118.王璋.1999.食品化学.北京:化学工业出版社,568-570
119.王振宇,杨谦.2003.大花葵红色素理化性质的研究.林产化学与工业,23(3):102-104
120.王振宇,杨谦.2005.微生物破壁法提取大花葵花色苷.东北林业大学学报,33(1):56-57
121.王志祥.1994.生化分离技术-制备型高效液相色谱.现代化工,9:18-20
122.王忠合,朱俊晨,陈惠音.2007.双层原花青素微胶囊的试验研究.食品科学,28(1):102-106
123.魏莉.2005.制备型高效液相色谱技术的研究进展.广东化工,11:5-7
124.吴朝霞,孟宪军,林琳.2005.几种微胶囊包裹方法对葡萄籽原花青素包裹效果的对比研究.食品工业科技,26(9):67-72
125.吴春,陈林林,李健.2006.菟丝子黄酮的微胶囊化及释放性能研究.农业工程学报,22(9):264-266
126.吴东生,金磊.1997.食品工业中的喷雾干燥法微胶囊.食品工业科技,17(1):78-82
127.吴恩岐.2002.花瓣花色素的定性分析.内蒙古师范大学学报,31(1):64-67
128.吴满平.1988.载脂蛋白基因和功能研究.生理科学进展,19(2):135
129.吴文林,胡天喜.1997.几种黄酮类化合物对羟自由基引起的DNA损伤的保护作用.自由基生命科学进展,(5):101-104
130.吴信子,朴京一,张小勇,等.2001.蓝靛果花青索的分离与鉴定.延边大学学报,27(3):191-194
131.肖道安,李秋红,陈芳.2007.杜仲叶提取物微胶囊化研究.赣南医学院学报,27(3):321-323
132.谢棒祥,张敏红.2003.生物类黄酮的生理功能极其应用研究进展.动物营养学报,15(2):11-15
133.谢凤霞,邱祖民,涂盛辉,等.2005.浸提法与超声波法提取法提取栀子黄色素的比较.南昌大学学报(理科版),29(3):278-281
134.熊何健,马玉婵,吴国宏.2005.茶多酚的提取及微胶囊化工艺研究.食品科学,26(10):137-140.
135.徐建国,田呈瑞,胡青平.2005.超声波提取桑葚红色素的工艺研究.山西农业大学学报,25(4):380-382
136.徐金瑞,张名位,刘兴华,等.2005.黑大豆种皮花色苷的提取及其抗氧化作用研究.农业工程学报,21(8):161-164
137.徐金瑞,张名位,刘兴华,等.2006.大孔树脂对黑大豆种皮花色苷的纯化研究.农业机械学报,37(4):145-148
138.徐俐,金毅,王斌,等.2006.树莓红色素的提取及稳定性的研究.食品科学,27(5):191-194
139.徐璐,郑建仙.2005.欧洲越桔花色苷的研究概况.中国食品添加剂,(4):43-46
140.徐清海,明霞.2005.天然色素的提取及其生理功能研究.应用化工,34(5):268-270
141.徐文秀,吴彩娥,李强,等.2006.丁香油喷雾干燥微胶囊技术研究.食品科学,27(1):278-281
142.徐渊金,杜琪珍.2006.花色苷分离鉴定方法及其生物活性.食品与发酵工业,32(3):67-72
143.徐忠,张亚丽.2002.D101大孔树脂对萝卜红色素的吸附特性研究.食品科学,(1):59-60
144.许申鸿.2000.天竺葵花抗氧化作用的研究.中国药学杂志,35(4):234-236
145.薛伟明,张效林.1999.红花黄色素的酶法提取应用研究.化学工程,27(1):42-44
146.闫巧娟,韩鲁佳,江正强.纤维素酶法提取芪多糖.中草药,2005,36(12):1804-1807
147.阎师杰,吴彩娥,寇晓虹,等.2003.核桃油微胶囊化工艺的研究.农业工程学报,19(1):168-171
148.杨桂霞.2004.笃斯越橘花色素的分离鉴定及栽培品种花色素的定量分析.吉林农业大学硕士论文.11-26
149.杨桂霞,范海林,李亚东,等.2005.RP--HPLC法测定栽培种越橘果中花色素苷的含量.药物分析杂志,25(10):1222-1224
150.杨小兰,毛立新,张晓云.2005.黑桑椹对高脂血症大鼠的降脂作用研究.食品科学,26(9),509-510
151.杨政水,罗显华,黄静,等.2005.商陆色素的稳定性研究.食品科学,26(9):104-107
152.杨转琴,王磊,范娜,等.2006.柚皮黄酮化合物含量及抗氧化活性的研究.食品科学,27(4):102-105
153.姚伙旺,曾秋平,劳永民,等.2006.食品添加剂对荔枝果皮花色素苷稳定性的影响.食品科学,27(5):152-156
154.叶兴乾,陈健初,苏平.1994.荸荠种杨梅的花色苷组分鉴定.浙江农业大学学报,20(2):188-190
155.于守洋,崔洪斌.2001.中国保健食品的进展,北京:人民卫生出版社,87-89
156.于晓南,张启翔.2002.观赏植物的花色素苷与花色.林业科学,38(3):147-153
157.于泽源,徐雅琴,李兴国.2004.草原樱桃红色素稳定性的研究.食品科学,25(6):111-113
158.余华.2004.玫瑰茄红色素的结构定性研究.食品科技,28(7):55-56
159.张尔贤,俞丽君,周意林,等.1996.Fe~(2+)诱发脂蛋白PUFA过氧化体系及对若干天然产物抗氧化作用的评价.生物化学与生物物理学报,28(2):218-292
160.张捷莉,马天芳,李铁纯,等.2005.板栗皮棕色素的提取与稳定性研究.食品科学,26(9):281-284
161.张峻,吉伟之,陈晓云,等.2002.吸附析法制备低聚原花青素.天然产物研究与开发,14(4):31-33
162.张峻,吉伟之,陈晓云,等.2004.壳聚糖-海藻酸钠微胶囊对葡萄多酚控制释放的研究.食品科学,25(5):102-104
163.张立娟,于国平,周国华.2005.黑木耳多糖酶法提取条件的研究.食品研究与开发,26(3):89-91
164.张连富,杜彦山,牟德华.2006.原花青素的微胶囊化研究.食品与发酵工业,32(2):64-66
165.张名位,郭宝江,张瑞芬,等.2006a.黑米抗氧化活性成分的分离纯化和结构鉴定.中国农业科学,39(1):153-160
166.张名位,张瑞芬,郭宝江,等.2006b.黑米皮提取物的抗氧化与降血脂作用及其成分分析.中国农业科学,39(11):2368-2373
167.张沙艳,闫玉杰.1999.高粱壳中色素提取及稳定性的研究.辽宁师专学报,(1):88-90
168.张世东,王建建,王霞,等.2007.玉米须单体成分纯化及类别鉴定.福建分析测试,16(3):67-69
169.张树宝.2006.超临界CO_2萃取大花葵花色苷工艺研究.特产研究,(1):29-33
170.张惟杰主编.1999.糖复合物生化研究技术.浙江大学出版社:102-105.
171.张燕,谢玫珍,廖小军.2005.热和紫外辐射对红莓花色苷稳定性的影响.食品与发酵工业,31(3):37-40
172.张跃超.2001.果树新品-美国蓝莓.农业科技通讯,(1):16
173.张钟,宫坤,陈守江.2004.黑糯玉米色素的提取及性质研究.中国粮油学报,19(2):62-71
174.张钟,张志海.2006.影响黑糯玉米芯色素抗氧化活性的因素及与其他抗氧化剂效应的比较.食品与发酵工业,30一33
175.赵昶灵,郭维明,陈俊愉.2004.梅花“南京红”花色色素花色苷的分子结构.云南植物研究,26(5):549-557
176.赵晓燕,冯作山,陈复生.2005.番茄红素微胶囊的稳定性研究.食品与发酵工业,31(10):45-47.
177.赵宜江,姚建民.1997.无机膜提取栀子黄色素的工艺研究.南京化工大学学报,19(1):77-81
178.郑光耀.葡萄籽原花青素提取物的生理活性、药用作用及其应用.林产化工通讯,2000,34(1):28-33
179.郑穗平,沈继红,郭勇.1997.利用玫瑰茄细胞悬浮培养技术生产花色苷的初步研究.食品工业科 技,(6):29-31
180.钟丽玉.黑米分子结构解析.无锡轻工大学学报,1996,15(1):33-38
181.周宇,闫国华,张开春,等.2006.桔梗花色苷的提取与稳定性研究.食品工业科技,27(3):162-165
182.朱蓓微,金英实.2003.提高越桔天然色素稳定性方法的研究.食品科学,24(5):81-84
183.朱洪梅,韩永斌,顾振新,等.2006a.大孔树脂对甘薯色素的吸附与解吸特性研究.农业工程学报,22(5):153-156
184.朱洪梅,韩永斌,顾振新,等.2006b.单宁对紫甘薯花色苷的辅色作用的研究.南京农业大学学报,29(3):98-102
185.朱文学,吴龙奇,易军鹏,等.2006.牡丹花色素理化性质的研究.农业工程学报,22(6):224-226
186.朱选,阳会军,黄慧敏,等.2000.β-胡萝卜素微胶囊化工艺参数的研究.食品与机械,(5):11-13
187.朱英,罗永明,付小梅.1989.大孔树脂分离油茶皂甙和黄酮的研究.林产化学与工业,19(1):40-42
188.Aaby K,Skrede G,and Wrolstad R E.2005.Phenolic composition and antioxidant activities in flesh and achenes of strawberries(Fragaria ananassa).J.Agric.Food Chem.,53(10):4032-4040
189.Annamaryin D S.1997.Antioxidant ability of anthocyanins against ascorbic acid oxidation,Phytochemistry.45(4):671-674
190.Armstrong M L.1976.Evidence of regression of atherosclerosis in primates and man.Postgrad.Med.52,456
191.Assmann G,Gotto A M.2004.HDL cholesterol and protective factors in atherosclerosis.Circulation,109:Ⅲ8-14
192.Bagchi D,Sen chandan K,Ray S D,et al.2003.Molecular mechanisms of cardioprotection by a novel grape seed proantocyanidin extract.Mutation Research.523-524.
193.Bao J S,Cai Y Z,Sun M,et al.2005.Anthocyanins,Flavonols,and free rdical scavenging activity of Chinese bayberry(Myrica rubra) extracts and their colour properties and stability.J.Agric.Food Chem.,53(6):2327-2332
194.Bate-Smith E C.1948.Paper chromatography of anthocyanins and related substances in petal extracts.Nature,(4100):835-838
195.Brown M S,Faust J R,and Goldstein J L.1975.Role of the low density lipoprotein receptor in regulating the contents of free and esterified cholesterol in human fibroblasts.J.Clin.Invest.55,783
196.Cao G,Sofic E,Prior R L.1997.Antioxidant and prooxidant behavior of flavoniods:structure-activity relation.Free Redical Biology and Medicine.22(5):746-760
197.Cao L.1994.Quantification and distribution of simple and acylated anthocyanins and other phenolics in blueberries.Food science,59:1057-1059.
198.Castro L,and Freeman B.2001.Reactive oxygen species in human health and disease.Nutrition.17:161-165
199.Chandra A,Rana J,Li Y Q.2001.Separation,idertification,quantification,and method validation of anthocyanins in botanical supplement raw materials by HPLC and HPLC-MS.J.Agric.Food Chem.,49(8):3515-3521
200.Charron C S,Clevidence B A,Britz S J,et al.2007.Effect of dose size on bilavailability of acylated and nonacylated anthocyanins from red cabbage(Brassica oleracea L.Var.capitata).J.Agric.Food Chem.,55(13):5354-5362
201.Chen L J,Hrazdina G.1981.Structural aspects of anthocyanin flavonoid complex formation and its role in plant color.Phythchem.,20:297-303
202.Chou E J.2001.Effect of ingestion of purple grae juice on endothelial function in patieuts with coronary heart disease.The American Journal of Cardiology,88(9):553-555
203.Connor A.M.,et al.2002.Coenotypic and environmental variation in antioxidant activity,totalphenolic content and anthocyanin content among blueberry cultivars,Amer.Soc.Hort.Sci,127(1):89-97.
204.Cooke D N,Thomasset S,Boocock D J,et al.2006.Development of analyses by high-performance liquid chromatography and liquid chromatography/tandem mass spectrometry of bilberry(Vaccinium myrtilus) anthocyanins in human plama and urine.J.Agric.Food Chem.,54(19):7009-7013
205.Dangles O,Saito N,Brouillard R.1993.Anthocyanin intramolecular copigment effect.Phytochemistry.34:119-124
206.Degenhardt A,Knapp H,Winterhalter P.2000.Separation and purification of anthocyanins by high-speed countercurrent chromatography and screening for antioxidant ctivity.J.Agric.Food Chem.,48(2):338-343
207.Di Paola-Naranjo R D,Sanchez-Sanchez J,Gonzalez-Paramas A M,et al.2004.Liquid chromatographic-mass spectrometric analysis of anthocyanin composition of dark blue bee pollen from Echium plantagineum.J Chromatogr A.,1054(1-2):205-210
208.Duh P D,YenG C.1997.Antixidant activities of three herbal water extracts.Food Chem,60:639-645
209.Ghiselli A,Nardini M,Baldi A.,et al.1998.Antioxidant activity of different phenolic fractions separated from An Italian red wine,J.Agric.Food Chem.,46:361-367
210.Hanson C.1999.The advantage of natural colors in food products.Food Marketing and Technology,99:12-15
211.Harborne J B.1958a.Spectral methods of characterizing anthoeyanins.J.Bio.Chem,70:22
212.Harborne J B.1958b.The chromatographic identification of anthoeyanin pigments.Journal of Chromatography,1:473-488
213.He J,Magnuson B A,and Giusti M M.2005.Analysis of anthoeyanins in rats intestinal contents-impact of anthocyanins chemical structure on fecal excretion.J.Agric.Food Chem.,53(8):2859-2866
214.Heinonen I M,Meyer A S,and Frankel E N.1998.Antioxidant activity of berry phenolics on human low density lipoprotein and liposome oxidation.J.Agrie.Food Chem.,46:4107-4112
215.Hennink W E,Nostrum C F.2002.Novel cross-linking methods to design hydrogels.Advanced Drug Delivery Reviews,54:13-36
216.Heo H J and Lee C Y.2005.Strawberry and its anthoeyanins reduces oxidative stress-induced apoptosis in PC12 cells.J.Agric.Food Chem.,53(6):1984-1989
217.Iehiyanagi T,Shida Y,Rahman M M,et al.2006.Bioavailability and tissue distribution of anthoeyanins in bilberry(Vaccinium myrtillus L.) extract in rats.J.Agrie.Food Chem.,54(18):6578-6587
218.Iehiyangi T,Hatano Y,Matsugo S,et al.2004.Structural dependence of HPLC separation pattern of anthocyanins from Bilberry(Vaceinium myrtillus L.).Chem Pharm Bull(Tokyo),52(5):628-630
219.Igarashi K,Kimura Y,Takenaka A.2000.Preventive effects of detary cabbage acylated ant-hocyanins on paraqual-induced oxidative stress in rats.Biosci,Biotechnol,Biochem.64:1600-1607
220.Joseph A.B.1973.Microencapsulation of foods and related products.Food technology,(11):34-44
221.Jung C H,Seog H M,Choi I W,et al.2005.Antioxiant activities of cultivated and wild Korean ginseng leaves.Food Chemistry,92:535-540
222.Kalt W and Mcdonald J E.1996.Chemical composition of lowbush blueberry cultivars.J.Amer.Soc.Hort.Sci.;121(1):142-146.
223.Kalt W,Forney C F,Martin A,et al.1999.Antioxidant,capacity,vitarninC,phenolics,and anthocyanins after fresh storage of small fruits.J.Agric.Food chem.,47,4638-4644.
224.Kalt W,Blumber J B,McDonald J E,et al.2008.Identification of anthocyanins in the liver,eye,and brain of blueberry-fed pigs.J Agric Food Chem.,56(3):705-712.
225.Katsube N,Iwashita K,Tsushida T,et al.2003.Induction of apoptosis in cancer cells by bilberry(Vaccinium myrtillus) and the anthocyanins.J.Agric.Food Chem.,51(1):68-752
26.Kayamori F,Igarashi K.1994.Effects of dietary nasunio on the serum cholesterol level in rats.Biosci.Biotechnol.Biochem.58:507-571.
227.Kim M J,Hyun J N,Kim J A,et al.2007.Relationship between phenolic compounds,anthocyanins content and antioxidant activity in colored barley germplasm.J.Agric.Food Chem.55(12):4802-4809
228.Lapidot T,Hurel S,Akiri B,et al.1999.pH-dependent forms of red wine anthocyanins as antioxidants.J.Agric.Food Chem.47:67-70
229.Lapidot T,Harel S,Granit R,et al.1998.Bioavailability of red wine anthocyanins as detected in human urine.J.A gric.Food Chem.,46910):4297-4302.
230.Laplaud P M,Lelubre A,and Chapman M J.1997.Antioxidant of total monomeric anthocyanin pigment content of fruits juices,beverages,natural colorants,and wines by the pH different method:collaborative study.J AOAC Int,88(5):1269-1278
231.Lau F C,Shukitt-Hale B and Joseph J A.2005.The beneficial effects of fruit polyphenols on brain aging.Neurobiology of aging,26(1):128-132
232.Lee J and Wrolstad R E.2004.Extraction of anthocyanins and polyphenolics from blueberry processing waste.Journal of food science,69(7):564-573
233.Lee L,Durst R W,Wrolatad R E.2005.Determination of total monomeric anthocyanin pigment content of fruit juices,beverages,natural colorants,and wines by the pH differental method:collaborative study.J AOAC int,88(5):1269-1278
234.Liu X D,Takuroh Atarashi,Takeshi Furuta,et al.2001.Microencapsulation of emulsified hydrophobic flavors by spray drying.Drying Technology.19(7):1361-1374
235.Ma Y H,Li W L,Peng Y F,et al.2003.The extractive technique of red pigment of Bougainvillea spectabilis wild.Food Sciences,24(1):78-80
236.Matsumoto H,Hanamra S,Kawakami T et al.2001.Preparative-scale isolation of four anthocyanin components of black current(Ribes nigrum L.)fruits.J.Agric.Food Chem.,49(3):1541-1545
237.Maurizio F.1995.Preparative high-performance liquid chromatography for the purification of natural anthocyanins.Jourhal of Chromatography A,(692):213-219
238.Mazza G,Cacace J E,Kay C D.2004.Methods of analysis for anthocyanins in plants and biological fluits.J AOAC int,87(1):129-145
239.Mazza G,Kay C D,Cottrell T,et al.2002.Absorption of anthocyanins from blueberries and serum antioxidant status in human subjects.J.Agric.Food Chem.,50(26):7731-7737
240.Mazza G.1987.Recent Development in the stabilization of anthocyanins in food products.Food Chemistry,25:207-225
241.McDougall G J,Dobson P,Smith P,et al.2005.Assessing potential bioavability of raspberry anthocyanins using an in vitro digestion system.J.Agric.Food Chem.,53(10):5896-5904
242.McGhie T K,Ainge G D,Barnett L E,et al.2003.Anthocyanins glycosides from berry fruits are absorbed and excreted unmetabolized by both humans and rats.J.Agric.Food Chem.,51(16):4539-4548
243.McGhie T K,Rowan D R,Edwards P J.2006.Structural identification of two major anthocyanins components of boysenberry by NMR spectroscopy J.Agric.Food Chem.,54(23):8756-8761
244.Milbury P E,Cao G H,Prior R L.2002,Bioavailablility of elderberry anthocyanins.Mechanisms of Ageing and Development,123:997-1006
245.Motoori S.1998The Food Industry,41(16):22-28
246.Moyer R A,Hummer K E,Finn C E,Wrolstad R E.2002.Anthocyanins,phenolics,and antioxidant capacity in diverse small fruits:Vaccinium,Rubis,and Ribes.J.Agric.FoodChem.,50:519-525
247.Nakano T,Nakano K,Sim J S.1998.Extraction of glycosaminoglycan peptide from bovine nasal cartilage with 0.1M sodium acetate.J.Agric Food Chem,(46):772-778.
248.Nielsen I LF,Dragsted L O,Ravn-Haren G,et al.2003.Absorption and excretion of black currant anthocyanins in humans and watanabe heritable hypedipidemie rabbits.J.Agric.Food Chem.,51(9):2813-2820
249.Norio Sito,Kenjiro Toki,Hidekazu Moriyama.2002.Acylated anthocyanins from the blue-violet flowers of anemone coronaria.Phytochemistry.60:365-373
250.Nyman N A and Kumpulainen J T.determination of anthocyanins in berries and red wine by High-performance liquid chromatography.J.Agric.Food Chem.,2001,49(9):4183-4187
251.Obedeg L W.1986.Free radicals aging and degenerative disease.Alan R Liss Inc,42:301-311
252.Ock-Sook Yi.1997.Antioxidant activity of grape extracts in a lecthin liposome system.JAOCS,74(10):1301-1306
253.Olsson M E,Gustawsson K E,Andersson S,et al.2004.Inhibition of cancer cell proliferation in Vitro by fruit and berry extracts and correlation with antioxidant levels.J.Agric.Food Chem.,52(24):7264-7271
254.Osawa Y.Anthocyanins as food colors.New York:academic Press,1982,85
255.Otukia T,Matsufujia H,Takedaa M.2002.Acylated anthocyanins from red radish.Phytochemistry.60:79-87
256.Pauleti M S,Mestoy P.1999.Butter microencapsulation as affected by composition of wall material and fat.J.Food Sci.64(2):279
257.Pinelo M,Rubilar M,Jerez M,et al.2005.Effect of solvent,temperature,and solvent-to-solid ratio on the total phenolic content and antiradical activity of extracts from different components of grape pomace.J.Agric.Food Chem.,53(6):2111-2117
258.Prior R L,Lazarus S A,Cao G H,et al.2001.Identification of procyanidins and anthocynins in blueberries and cranberries(Vaccinium SPP.) using high-performance liquid chromatography/Mass spectrometry.J.Agric.Food Chem.,49(3):1270-1276
259.Prior R L,Wu X,Gu L,et al.2008.Whole berries versus berry anthocyanins:interactions with dietary fat levels in the C57BL/6J mouse model of obesity.J Agric Food Chem.,56(3):647-653.
260.Quan Q z,Dang R Y,Li T S.2001.Adsorbing and separating radish red pigment by X-5 Resin.Acta Botanica Boreal-Occidentalia Sinica,21(6):1218-1222.
261.Renand S,Zorgelil M.1992.Wine,alcohol,platelets and the French paradox for coronary heart disease.Lancet.33(9):1523-1526
262.Saito N,Tatsuzawa F,Yoda K,et al.1995.Acylated cyaniding glycosides in the viole t-blue flowers of ipomoea purpurea.Phytochemistry.40(4):1283-12 8 9
263.Satue Gracia M T,Heinonen M,Frankel E N.1997.Anthocyanins as antioxidants on human low-density lipoprotein and lecithin-liposome systems.J.Agric Food Chem,45(3):362-367.
264.Schwarz M,Hillebrand S,Habben S,Degenhardt A,et al.2003.Application of high-speed counter-current chromatography to the large-scale isolation of anthocyanins.Biochem Eng J,14(3):179-189
265.Seeram N P,Adams L S,Zhang Y J et al.2006.Blackberry black raspberry,blueberry,cranberry,red raspberry,and strawberry extracts inhibit growth and stimulate apoptosis of human cancer cells in vitro.J.Agric.Food Chem.,54(25):9329-9339
266.Shahidi F,Han X Q.1993.Encapsulation of food ingredients.Critical Review Food Sci.Nutri.33(6):501.
267.Smironff N,Cumbes Q J.1989.Hyroxyl radical scavenging activity of compatible solutes.Phytochemistry,28(4):1051-1560
268.Solomon A,Golubowicz S,Yablowicz Z,et al.2006.Antioxidant activities and anthocyanin content of fresh fruits of common Fig(Ficus carica L.).J.Agric.Food Chem.,54(20):7717-7723
269.Suda I,Oki T,Masuda M,et al.2002,Direct absorption of acylated anthocyanin in purple- fleshed sweet potato into rats.J.Agric.Food Chem.,50(6):1672-1676
270.Talavera S,Felgines C,Texier O,et al.2005.Anthocyanin metabolism in rats and their distribution to digestive area,Kidney,and brain.J.Agric.Food Chem.,53(10):3902-3908
271.Tan BKH.1991.Sixth SEA-Western Pacific Regional Meeting of Pharmacologists.Hong Kong:234.
272.Terasawa N,Saotome A,Tachimura Y,et al.2007.Identification and some properties of anthocyanin isolated from zuiki,Stalk of colocasia esculenta.J.Agric.Food Chem.55(10):4154-4159
273.Tian Q G,Giusti M M,Stoner G D,et al.2005.Schwartz.Screening for anthocyanins using high-performance liquid chromatography coupled to electrospray ionization tandem mass.Journal of Chromatography A,109(1-2):72-82
274.Tian Q G,Giusti M M,Stoner G D,et al.2006.Urinart excretion of black raspberry(Rubus occidentalis) anthocyanins and their metabolites.J.Agric.Food Chem.,54(4):1467-1472
275.Tomas J P,Geiger PG,Girotti AM.1993.Lethal Damage to endothelial cell by oxidized low density lipoprotein:role of selenoperoxidase in cyctoprotection agains lipid hydroperoperoxide-and iron-mediated reactions.Lipid Res,479-490
276.Tsuda T.1994.Antioxidntive Activity of Anthoocyanin Pigments Cyanidin 3- O-β-D-gluceside and Cyandin,J.Agre.Food Chem.42(11):2407-2410
277.Tsuda T,Horio F,Osawa T.1999.Absorption and metabolism of cyanidin3-O-β-D-gl ucoside in rats.FEBS Letters,449:179-I82
278.Tsuda T,Watanade M,Ohshima K,et al.1994.Antioxidative activity of the anthocyanin pigments cyanidin3-O-β-D-Glucoside and cyaniding.J Agric.Food Chem,42(10):2407-2410
279.Vander Meer R,De Vrises H T,West C E,et al.Casein-induced hypercholesterolaemia in rabbits is calcium-dependent.Atherosclerosis,1985,56:139-147
280.Vinson J A.2001.Red wine,dealcoholized red wine and especially grape juice inhibit atherosclerusis in a hamster mode.Atherosclerosis.156:67-72
281.Voyiaziks E,Glodberg I J,Plump A S,et al.PapoA triglyceridemia and increased atherosclerosis in human apoB transgenic mice.J lipid Res1998,39(2):313-321
282.Wang H,Cao G H,and Prior R L.1997.Oxygen radical absorbing capacity of anthocyanins.J.Agric.Food Chem.,45,304-309.
283.Wang H,Liu G,Yang L,et al.2005.Effects of Liensinine on blood lipids and lipid peroxidation in experimental hyperlipidemia rats.Natural Product Research and Development.17(6):722-725.
284.Wang J and Mazza G.2002.Effects of anthocyanins and other phenolic compounds on the production of tumor necrosis factor α in LPS/IFN-γ-activated RAW 264.7 macrophages.J.Agric.Food Chem,50(15):4183-4189
285.Wang Y,Chen F C,Jenny C,et al.2005.Dietary supplementation with blueberries,spinach,or spirulina reduces ischemic brain damage.Experimental Neurology, 193(1):75-84
286.Wilson T, Porcari J P, Harbin D.1998.Cranberry extract inhibits low density lipoprotein oxidantion.Life Science, 62 (24):381-386
287.Wu Q K., Koponen J M, Mykkanen H M, et al.2007.Berry phenolic extracts modulate the expression of p21~(WAFI) and bax but bcl-2 in HT-29 colon cancer cells.J.Agric.Food Chem.55(4):1156-1163
288.Wu X L and Prior RL.2005.Systematic identification and characterization of anthocyanins by HPLC-ESI-MS/MS in common foods in the United States: Fruits and berries.J.Agric.Food Chem., 53(7):2589-2599
289.Wu X L, Beecher G R, Holden J M, et al.2006.Concentrations of anthocyanins in common foods in the Uniteds States and estimation of normal consumption.J.Agric.Food Chem., 54(11):4069-4075
290.Yen G C , Chen H Y.1995.Antioxidant activity of various tea extracts in reation to their antimutagenicity.J Agric Food Chem, 43(1):27-32
291.Yi W G, Akoh C C, Fischer J ,et al.2006a.Effects of phenolic compounds in blueberries and muscadine grapes on HepG2 cell viability and apoptosis.Food Research International, 39(5):628-638
292.Yi W G , Akoh C C, Fischer J, et al.2006b.Absorption of anthocyanins from blueberry extracts by caco-2 human intestinal cell monolyers.J.Agric.Food Chem., 54(15):5651-5658
293.Yi W G , Fischer J, Krewer G, et al.2005 .Phenolic compounds from blueberries can inhibit colon cancer cell proliferation and induce apoptosis.J.Agric.Food Chem., 53(18):7320-7329
294.Youdim K.A, Mcdonald J, Kalt W, et al.2002.Potential role of dietary flavonoids in reducing microvascular endothelium vulnerability to oxidative and inflammatory insults.the Journal of Nutritionall Biochemistry, 13(5):282-288
295.Zanatta C F.Cuevas E, Bobbio F O, et al.2005.Determination of anthocyanins from cmu-camu (Myrciaria dubia) by HPLC-PDA.HPLC-MS, and NMR.53(24):9531-9535
296.Zhang Z, Kou X L, Fugal K, et al.2004.Comparison of HPLC methods for determination of anthocyanins and anthocyaninidins in bilberry extracts.J.Agric.Food Chem., 52(4):688-691
297.Zhao C W, Giusti MM, Malik M,et al.2004.Effects of commercial anthocyanin-rich extracts on colonic cancer and nontumorigenic colonic cell growth.J.Agric.Food Chem., 52(20):6122-6128
298.Zheng W and Wang S Y.2003.Oxygen radical absorbing cpacity of phenolics in blueberries,cranberries,chokeberries, and lingonberries.J.Agric.Food Chem., 51(2):502-509
299.Zhu L S, Ma X C, Liu,et al.2004.Effects of total flavones of buckwheat leaf on blood lipid and lipid peroxides.Chines Journal rehabilitation, 8(24):5178-5179