中药对运动大鼠抗疲劳能力及脑自由基的影响
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摘要
1.研究目的
通过对服用补肾益气中药及运动训练大鼠的运动能力、脑组织自由基、抗氧化剂及抗氧化酶等指标的变化的分析,探讨自由基与运动性中枢疲劳产生的关系及中草药抵抗运动性中枢疲劳的机制,为科学的指导运动训练和健身活动、快速有效的消除运动性疲劳并增强运动能力、开发抗疲劳中药制剂提供重要的科学理论依据。
2.研究方法
选取8周龄健康雄性Sprague-Dawley(SD)大鼠120只,随机分为对照组(C组)、服药组(M组)、训练组(T组)和训练服药组(TM组)。对M组和TM组大鼠灌服中药水煎液,对C组和T组大鼠灌服等量纯净水,约1ml/100g体重,1次/天;对T组和TM组大鼠进行递增负荷的游泳训练5周,每周训练6天,休息1天。5周后对大鼠进行取材,每组大鼠按处死状态(安静、力竭即刻、力竭恢复12小时)又分为三个亚组,分别测定大鼠脑组织一氧化氮(NO)、丙二醛(MDA)、谷胱甘肽(GSH)的含量和超氧化物歧化酶(SOD)的活力。所有数据经SPSS13.0统计软件进行统计分析。
3.实验结果
3.1在整个实验期间,四组大鼠精神饱满,活泼好动,皮毛光洁整齐,眼睛有神;未出现倦怠懒动,双眼黯淡无光,眯眼弓背,体形瘦弱,毛杂乱无光泽,甚至皮毛脱落等症状。各组大鼠体重均呈稳定增长趋势。
3.2与对照组相比,其它三组大鼠力竭时间明显延长,且运动服药组延长的时间最为显著(P＜0.01)。
3.3安静状态下,NO和MDA含量变化一致,服药组、运动组和运动服药组与对照组相比均降低,运动服药组降低幅度最大;SOD活性与对照组相比,服药组和运动服药组降低,运动组升高;GSH含量与对照组相比,其它各组均降低。
3.4力竭即刻状态下,NO和MDA含量,服药组、运动组和运动服药组与对照组相比均降低;SOD活性与对照组相比,服药组和运动服药组降低,运动组升高;GSH含量与对照组相比,服药组和运动服药组减少,运动组增加。
3.5力竭恢复12小时状态下,NO和MDA含量变化一致,服药组、运动组和运动服药组与对照组相比均降低,运动服药组降低幅度最大;SOD活性与对照组相比服药组稍升高,运动组稍降低,但都无统计学意义,运动服药组明显降低;GSH含量与对照组相比,服药组升高,运动组和运动服药组降低。
4.结论
4.1补肾益气中药和运动训练能有效提高大鼠的运动能力,明显延长大鼠游泳至力竭的时间,且服用补肾益气中药和运动训练相结合效果更佳。
4.2补肾益气中药和运动训练使大鼠脑组织自由基一氧化氮和脂质过氧化产物丙二醛含量明显降低,且服药和运动相结合降低幅度更大,说明中药和运动训练能抑制脂质过氧化反应,减少脑组织自由基一氧化氮的生成。
4.3补肾益气中药和运动训练对大鼠脑组织超氧化物歧化酶活性和谷胱甘肽含量的影响在本实验中没有表现出一定的规律性,补肾益气中药和长期中等强度的递增负荷训练对大鼠脑组织抗氧化剂和抗氧化酶影响的机理仍需进一步探讨。
Objective:
This research tested exercise ability、free radical、antioxidant and antioxidant enzyme in the brain of big rats which drunk the traditional Chinese medicine for benefiting kidney and profiting spleen and trained, through analyzing the change of above indexes to explore the relation between free radical and exercise-induced central fatigue and the mechanism of traditional Chinese medicine preparation resist exercise-induced central fatigue. In order to provide the scientific theory basis to guide sports training and exercises to keep in good health, to eliminate exercise-induced fatigue and enhance exercise ability, to make anti-fatigue traditional Chinese medicine preparation.
Method:
Selected 120 healthy male Sprague-Dawley big rats which were 8-week-old, the big rats were randomly divided into contrast group (group C), drinking medicine group (group M), training group (group T), training and drinking medicine group (group TM). The big rats drunk traditional Chinese medicine in group M and group TM and drunk pure water in group C and group T, about 1ml/100g body weight, once/one day; The big rats carried out progressive resistance swimming training five weeks in group T and group TM, the big rats trained six days and rested one day every week. The kidneys were taken from the body of big rats after five weeks. Each group rats were divided into three inferior groups again according to the state (quite, exhaustion immediately, recover 12 hours after exhaustion) which were executed, tested the content of NO、MDA、GSH and the activity of SOD in the brain of rats respectively. All dates were analyzed by SPSS13.0.
Results:
1 In the whole course of experience, four groups big rats are invigorating and lively, their fur is bright and tidy, their eyes are shining; no below the symptom appears, such as tired and sleepy, eyes faint, narrow eyes and bow back, body thin and weak, fur disorderly and no light, even fur drop. Each group big rats' weight is on the trend of steady groeth.
2 Compare with group C, the time of other three group big rats swim to exhaustion is obvious prolong, and this phenomenon is most obvious (P<0.01) in group TM.
3 Under the state of quiet, the change of NO and MDA content is consistent, compare with group C, the content all reduce in other three groups, the range of decrease is biggest in group TM; compare with group C, the activity of SOD both reduce in group M and group TM, the activity of SOD rise in group T; compare with group C, the content of GSH all reduce in other three groups.
4 Under the state of exhaustion immediately, compare with group C, the content of NO and MDA all reduce in group M、group T、group TM; compare with group C, the activity of SOD reduce and the content of GSH reduce in group M and group TM, the activity of SOD rise and the content of GSH increase in group T.
5 Under the state of recovering 12 hours after exhaustion, the change of NO and MDA content is consistent, compare with group C, the content all reduce in other three groups, the range of decrease is biggest in group TM; compare with group C, the activity of SOD rise a bit in group M and reduce a bit in group T, but those change hasn't significance in statistics, the activity of SOD obviously reduce in group TM; compare with group C, the content of GSH rise in group M and reduce in group T and group TM.
Conclusion:
1 The traditional Chinese medicine for benefiting kidney and profiting spleen and training can enhance the exercise ability of big rats effectively, prolong the time of big rats swim to exhaustion obviously, and the result of drinking medicine and combine training is better.
2 The traditional Chinese medicine for benefiting kidney and profiting spleen and training make the free radical NO and the outcome of lipid peroxidation MDA reduce obviously, and the range of decrease is biggest in drinking medicine and combine training group, those indicate that the traditional Chinese medicine preparation and training can inhibit the reaction of lipid peroxidation, reduce the generation of free radical NO in the brain.
3 The change of SOD activity and GSH content which are influenced by the traditional Chinese medicine for benefiting kidney and profiting spleen and training didn't show specified regulation in the brain of big rats in this experience, the mechanism that the traditional Chinese medicine for benefiting kidney and profiting spleen and long-period moderate intensity progressive resistance training influence the antioxidant and antioxidant enzyme remain need discussed further.
通过对服用补肾益气中药及运动训练大鼠的运动能力、脑组织自由基、抗氧化剂及抗氧化酶等指标的变化的分析,探讨自由基与运动性中枢疲劳产生的关系及中草药抵抗运动性中枢疲劳的机制,为科学的指导运动训练和健身活动、快速有效的消除运动性疲劳并增强运动能力、开发抗疲劳中药制剂提供重要的科学理论依据。
2.研究方法
选取8周龄健康雄性Sprague-Dawley(SD)大鼠120只,随机分为对照组(C组)、服药组(M组)、训练组(T组)和训练服药组(TM组)。对M组和TM组大鼠灌服中药水煎液,对C组和T组大鼠灌服等量纯净水,约1ml/100g体重,1次/天;对T组和TM组大鼠进行递增负荷的游泳训练5周,每周训练6天,休息1天。5周后对大鼠进行取材,每组大鼠按处死状态(安静、力竭即刻、力竭恢复12小时)又分为三个亚组,分别测定大鼠脑组织一氧化氮(NO)、丙二醛(MDA)、谷胱甘肽(GSH)的含量和超氧化物歧化酶(SOD)的活力。所有数据经SPSS13.0统计软件进行统计分析。
3.实验结果
3.1在整个实验期间,四组大鼠精神饱满,活泼好动,皮毛光洁整齐,眼睛有神;未出现倦怠懒动,双眼黯淡无光,眯眼弓背,体形瘦弱,毛杂乱无光泽,甚至皮毛脱落等症状。各组大鼠体重均呈稳定增长趋势。
3.2与对照组相比,其它三组大鼠力竭时间明显延长,且运动服药组延长的时间最为显著(P＜0.01)。
3.3安静状态下,NO和MDA含量变化一致,服药组、运动组和运动服药组与对照组相比均降低,运动服药组降低幅度最大;SOD活性与对照组相比,服药组和运动服药组降低,运动组升高;GSH含量与对照组相比,其它各组均降低。
3.4力竭即刻状态下,NO和MDA含量,服药组、运动组和运动服药组与对照组相比均降低;SOD活性与对照组相比,服药组和运动服药组降低,运动组升高;GSH含量与对照组相比,服药组和运动服药组减少,运动组增加。
3.5力竭恢复12小时状态下,NO和MDA含量变化一致,服药组、运动组和运动服药组与对照组相比均降低,运动服药组降低幅度最大;SOD活性与对照组相比服药组稍升高,运动组稍降低,但都无统计学意义,运动服药组明显降低;GSH含量与对照组相比,服药组升高,运动组和运动服药组降低。
4.结论
4.1补肾益气中药和运动训练能有效提高大鼠的运动能力,明显延长大鼠游泳至力竭的时间,且服用补肾益气中药和运动训练相结合效果更佳。
4.2补肾益气中药和运动训练使大鼠脑组织自由基一氧化氮和脂质过氧化产物丙二醛含量明显降低,且服药和运动相结合降低幅度更大,说明中药和运动训练能抑制脂质过氧化反应,减少脑组织自由基一氧化氮的生成。
4.3补肾益气中药和运动训练对大鼠脑组织超氧化物歧化酶活性和谷胱甘肽含量的影响在本实验中没有表现出一定的规律性,补肾益气中药和长期中等强度的递增负荷训练对大鼠脑组织抗氧化剂和抗氧化酶影响的机理仍需进一步探讨。
Objective:
This research tested exercise ability、free radical、antioxidant and antioxidant enzyme in the brain of big rats which drunk the traditional Chinese medicine for benefiting kidney and profiting spleen and trained, through analyzing the change of above indexes to explore the relation between free radical and exercise-induced central fatigue and the mechanism of traditional Chinese medicine preparation resist exercise-induced central fatigue. In order to provide the scientific theory basis to guide sports training and exercises to keep in good health, to eliminate exercise-induced fatigue and enhance exercise ability, to make anti-fatigue traditional Chinese medicine preparation.
Method:
Selected 120 healthy male Sprague-Dawley big rats which were 8-week-old, the big rats were randomly divided into contrast group (group C), drinking medicine group (group M), training group (group T), training and drinking medicine group (group TM). The big rats drunk traditional Chinese medicine in group M and group TM and drunk pure water in group C and group T, about 1ml/100g body weight, once/one day; The big rats carried out progressive resistance swimming training five weeks in group T and group TM, the big rats trained six days and rested one day every week. The kidneys were taken from the body of big rats after five weeks. Each group rats were divided into three inferior groups again according to the state (quite, exhaustion immediately, recover 12 hours after exhaustion) which were executed, tested the content of NO、MDA、GSH and the activity of SOD in the brain of rats respectively. All dates were analyzed by SPSS13.0.
Results:
1 In the whole course of experience, four groups big rats are invigorating and lively, their fur is bright and tidy, their eyes are shining; no below the symptom appears, such as tired and sleepy, eyes faint, narrow eyes and bow back, body thin and weak, fur disorderly and no light, even fur drop. Each group big rats' weight is on the trend of steady groeth.
2 Compare with group C, the time of other three group big rats swim to exhaustion is obvious prolong, and this phenomenon is most obvious (P<0.01) in group TM.
3 Under the state of quiet, the change of NO and MDA content is consistent, compare with group C, the content all reduce in other three groups, the range of decrease is biggest in group TM; compare with group C, the activity of SOD both reduce in group M and group TM, the activity of SOD rise in group T; compare with group C, the content of GSH all reduce in other three groups.
4 Under the state of exhaustion immediately, compare with group C, the content of NO and MDA all reduce in group M、group T、group TM; compare with group C, the activity of SOD reduce and the content of GSH reduce in group M and group TM, the activity of SOD rise and the content of GSH increase in group T.
5 Under the state of recovering 12 hours after exhaustion, the change of NO and MDA content is consistent, compare with group C, the content all reduce in other three groups, the range of decrease is biggest in group TM; compare with group C, the activity of SOD rise a bit in group M and reduce a bit in group T, but those change hasn't significance in statistics, the activity of SOD obviously reduce in group TM; compare with group C, the content of GSH rise in group M and reduce in group T and group TM.
Conclusion:
1 The traditional Chinese medicine for benefiting kidney and profiting spleen and training can enhance the exercise ability of big rats effectively, prolong the time of big rats swim to exhaustion obviously, and the result of drinking medicine and combine training is better.
2 The traditional Chinese medicine for benefiting kidney and profiting spleen and training make the free radical NO and the outcome of lipid peroxidation MDA reduce obviously, and the range of decrease is biggest in drinking medicine and combine training group, those indicate that the traditional Chinese medicine preparation and training can inhibit the reaction of lipid peroxidation, reduce the generation of free radical NO in the brain.
3 The change of SOD activity and GSH content which are influenced by the traditional Chinese medicine for benefiting kidney and profiting spleen and training didn't show specified regulation in the brain of big rats in this experience, the mechanism that the traditional Chinese medicine for benefiting kidney and profiting spleen and long-period moderate intensity progressive resistance training influence the antioxidant and antioxidant enzyme remain need discussed further.
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