磁场对大鼠股骨干骨折愈合中VEGF、FGF和TGF-β的影响
摘要
骨折作为临床上常见病、多发病之一,已严重影响到患者的生活质量,如何促进骨折愈合一直是人们研究的热点,目前关于骨折愈合的研究有很多种,包括各种物理、化学以及生物手段。有研究表明,恒强旋转磁场(RCSMF)可以促进成骨细胞的增殖和分化,对骨质疏松有一定治疗作用,但有关恒强旋转磁场在骨折愈合中的应用研究鲜有文献报道。本研究旨在通过运用恒强旋转磁场对股骨骨折大鼠进行干预治疗来研究其在骨折愈合中的作用。
实验选取2月龄、3月龄雄性SD大鼠80只,采用闭合性动物骨折模型造模方法,用髓机数表法将sD大鼠分成自然愈合对照组(c组)和磁场干预组(M组),将M组大鼠置于强度为0.4T的恒强旋转磁场中进行30分钟／天的干预治疗,每周6次。通过比较不同组别不同时点的sD大鼠骨折愈合情况来判断恒强旋转磁场在骨折愈合中的作用,并从组织学变化以及血管内皮生长因子(VEGF)、酸性成纤维生长因子(aFGF)、碱性成纤维生长因子(bFGF)以及转化生长因子(TGF-β)的变化情况来探讨恒强旋转磁场对骨折愈合影响的可能机制。
结果发现,2周干预组VEGF光密度值显著高于对照组(P<0.01),3周、4周干预组VEGF光密度值也显著高于对照组(P<0.05)：2周于预组bFGF光密度值、4周干预组aFGF光密度值和对照组相比具有显著性差异(P<0.01)：2周干预组TGF-β1光密度值显著高于对照组(P<0.05),4周干预组TGF-β2光密度值显著高于对照组(P<0.01),其余各组间无明显差异；HE染色结果显示干预组大鼠骨折愈合情况优于实验对照组。
本研究得出以下结论：与自然愈合相比,恒强旋转磁场干预能明显促进骨痂生长,促进骨折的修复；恒强旋转磁场干预能够促进VEGF诱导血管形成能力增加,恒强旋转磁场在2周和3周时显著促进bFGF的表达,在4周时促进aFGF的表达,可能与磁场干预促进不同时期骨细胞的有丝分裂有关。恒强旋转磁场在骨折后后2周促进TGF-β1的表达,可能与其促进问充质细胞增殖分化为软骨细胞和成骨细胞,参与骨折的修复有关：而在骨折后2、3和4周时显著促进TGF-β2的表达,提示磁场干预不仅使TGF-β2持续转化软骨细胞的能力增强,还可能与成骨细胞活性有关。
Erneture as one of the clinical common in jurys. have seriocsly affected the quality of life of the patients, how to promote the healing has always been the hot spot of the study, at present about how to promote the healing of the research has a lot of kinds, including all kinds of physical. chemical and biological met hods. Research has shown that constant strong rotating magnetie field can promote bone call proliferation and lifferentiation, for osteoporosis have certain treatment effert, but the applieation of constant strong rotating magnetic field in the research of fractore healing has few reports in the literature. This studs aims to throush the use of constant strong retating magnetic field in the Eracture healing of rats to study its iuteryention in the role of the Eracture healing.
We select elshty2months.3months of male SD rats only. use closer Eracture model for the research, with random access method to (?) the SD rats into the natural healing group Groue W and magnctic field interention group (M group). M rats will be set in0.4T constant strong rotating milenetic field in30minules/day intervention treatment. six times a week. Through comparing the different erouns of SD rats to judge constant strotig rotating magnetic field in the role of the fracture realing, and use the (?) method to compare the changes of VEGF. aFGF. bEGF. IGF β1and tGF-β2to explore the mechanism of constant strong rotating magnetic field in fracture healing.
The resulrs found that the VEGE OD value of M2is higher than that in The C2group (P<0.01). the OD value of M3, M4also siznifieaut (P<0.01); than that in natural group (P<0.05);2weaks and4wecks M group bFGF OD values compared with the C group have signifieant difference (P<0.01); M2TGF-β1OD value signifieantly higher than that in C group (P<0.05), the TGF-β2OD value of M4yrogp signilicantly higher than that in C group (P<0.01). and the rest of the group has no difference.
The study concluded that compared with the natural healing, constant strong rotating magnetic field can obviously promote the intervention callus growth Constatant strong intervention can promote VEGF rotating magnetic field induced the formation of blood vessels ability increases, constant strong roptating magnetic field in two weeks and three weeks in significantly promote bFGF expression, in the four weeks of promoting the expression of aFGF, Constant strong rotating magnetic field in the two weeks after the fracture promote TGF-β1expression, and may promote mesenchymal cells for the proliferation and differentiation of cartilage cells and the osteoblast and participate in the fracture of the relevant repair; And after3and4weeks of the fracture can significantly promote TGF-beta2expression, indicating that the magnetic field not only make intervention TGF-beta2continuous transformation of cartilage cells ability strengthens, may also has some relationship with the osteoblast activity.
实验选取2月龄、3月龄雄性SD大鼠80只,采用闭合性动物骨折模型造模方法,用髓机数表法将sD大鼠分成自然愈合对照组(c组)和磁场干预组(M组),将M组大鼠置于强度为0.4T的恒强旋转磁场中进行30分钟／天的干预治疗,每周6次。通过比较不同组别不同时点的sD大鼠骨折愈合情况来判断恒强旋转磁场在骨折愈合中的作用,并从组织学变化以及血管内皮生长因子(VEGF)、酸性成纤维生长因子(aFGF)、碱性成纤维生长因子(bFGF)以及转化生长因子(TGF-β)的变化情况来探讨恒强旋转磁场对骨折愈合影响的可能机制。
结果发现,2周干预组VEGF光密度值显著高于对照组(P<0.01),3周、4周干预组VEGF光密度值也显著高于对照组(P<0.05)：2周于预组bFGF光密度值、4周干预组aFGF光密度值和对照组相比具有显著性差异(P<0.01)：2周干预组TGF-β1光密度值显著高于对照组(P<0.05),4周干预组TGF-β2光密度值显著高于对照组(P<0.01),其余各组间无明显差异；HE染色结果显示干预组大鼠骨折愈合情况优于实验对照组。
本研究得出以下结论：与自然愈合相比,恒强旋转磁场干预能明显促进骨痂生长,促进骨折的修复；恒强旋转磁场干预能够促进VEGF诱导血管形成能力增加,恒强旋转磁场在2周和3周时显著促进bFGF的表达,在4周时促进aFGF的表达,可能与磁场干预促进不同时期骨细胞的有丝分裂有关。恒强旋转磁场在骨折后后2周促进TGF-β1的表达,可能与其促进问充质细胞增殖分化为软骨细胞和成骨细胞,参与骨折的修复有关：而在骨折后2、3和4周时显著促进TGF-β2的表达,提示磁场干预不仅使TGF-β2持续转化软骨细胞的能力增强,还可能与成骨细胞活性有关。
Erneture as one of the clinical common in jurys. have seriocsly affected the quality of life of the patients, how to promote the healing has always been the hot spot of the study, at present about how to promote the healing of the research has a lot of kinds, including all kinds of physical. chemical and biological met hods. Research has shown that constant strong rotating magnetie field can promote bone call proliferation and lifferentiation, for osteoporosis have certain treatment effert, but the applieation of constant strong rotating magnetic field in the research of fractore healing has few reports in the literature. This studs aims to throush the use of constant strong retating magnetic field in the Eracture healing of rats to study its iuteryention in the role of the Eracture healing.
We select elshty2months.3months of male SD rats only. use closer Eracture model for the research, with random access method to (?) the SD rats into the natural healing group Groue W and magnctic field interention group (M group). M rats will be set in0.4T constant strong rotating milenetic field in30minules/day intervention treatment. six times a week. Through comparing the different erouns of SD rats to judge constant strotig rotating magnetic field in the role of the fracture realing, and use the (?) method to compare the changes of VEGF. aFGF. bEGF. IGF β1and tGF-β2to explore the mechanism of constant strong rotating magnetic field in fracture healing.
The resulrs found that the VEGE OD value of M2is higher than that in The C2group (P<0.01). the OD value of M3, M4also siznifieaut (P<0.01); than that in natural group (P<0.05);2weaks and4wecks M group bFGF OD values compared with the C group have signifieant difference (P<0.01); M2TGF-β1OD value signifieantly higher than that in C group (P<0.05), the TGF-β2OD value of M4yrogp signilicantly higher than that in C group (P<0.01). and the rest of the group has no difference.
The study concluded that compared with the natural healing, constant strong rotating magnetic field can obviously promote the intervention callus growth Constatant strong intervention can promote VEGF rotating magnetic field induced the formation of blood vessels ability increases, constant strong roptating magnetic field in two weeks and three weeks in significantly promote bFGF expression, in the four weeks of promoting the expression of aFGF, Constant strong rotating magnetic field in the two weeks after the fracture promote TGF-β1expression, and may promote mesenchymal cells for the proliferation and differentiation of cartilage cells and the osteoblast and participate in the fracture of the relevant repair; And after3and4weeks of the fracture can significantly promote TGF-beta2expression, indicating that the magnetic field not only make intervention TGF-beta2continuous transformation of cartilage cells ability strengthens, may also has some relationship with the osteoblast activity.
引文
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