电针机同对导线间距离与输出参数的探讨
摘要
电针机是现代科技的产物,近年来许多强调不同治疗功能的电针机经常问世。电针机功能基本上不外是由输出的电压、电流、频率,加上不同的波形,或者再用交、直流电(即插上家用电源或使用于电池)的特性来达成特定治疗的效果,这些工作参数尤以频率最为重要。不同的频率是可以影响身体许多的功能,有镇痛、增大局部血流量等等不同的功效,低频与高频的刺激往往会有不同的治疗结果,更造成了多样化的治疗效果。刺激强度以电压为主要参数,也有以电流或电功率来表示,是以病人能够忍受的强度为主,这个强度是介于感觉阈及痛阈之间,过高或过低疗效会不佳。电针时间久了就容易出现耐受性,必须要再把刺激的量增强。电针机波形的调制是非常重要的,临床使用时可针对疾病的特征,选择合适的波形,以提高治疗成效。
电针机针对特定穴位上更显得穴位的优越与特异性,例如使用电针刺激内关和公孙穴,可以更有效的抑制了心脏交感神经活性,能使心脏跳动的速率变慢,实质上可以提升心脏副交感神经的活性,对健康人体来说,具有保护性的调节作用。
然而这些电针机强调的治疗作用,仅是以所谓的工作参数来论断治疗的功效。但是人体本身就含有特殊的生物电流,这些生物电流的流动可进行沟通、联系等等不同功能以进行各种的生理作用,对维持身体的健康,相当重要。人体本来就是个复杂的有机体,体液中含有溶液、电解质液等等不同可进行化学及生理作用的液体。体内组织、细胞的结构组成不一,电阻不会是处处相同的,电流更不会单纯以直线最短的距离前进,功率更是会随距离与电阻而散失,同条导线间的两根针灸的距离内究竟有什么特殊性、以及会有什么结果产生都是值得探讨的课题,因此设计此试验。
目的：
这是一项在人体外的试验,目的在测试两端导线间是否因距离而导致相关参数的改变?或者寻求两导线间是否存在一些特异的现象?尝试找出一些共同点,归纳出规律,做为日后进一步试验的参考。
方法：
1.平放深度超过一时的猪花肉,在两根针灸针柄处连上电针机的同对导线,插入猪花肉,两根针的距离为1cm,2cm,4cm,6cm,8cm,10cm,12cm,14cm,16cm,18cm,20cm等长度,使用交流电、直流电为电源、分别以电表测量其电压与频率值。2.取深度超过一时的瘦肉,其余如方法同方法1.,再分别量出电压与频率值。
成果：
花肉组测得的直流电电压大于瘦肉组。花肉组测得的交流电电压大于瘦肉组。瘦肉组测得频率值大于花肉组。花肉组加瘦肉组(即全部组数)结果
1.花肉组加瘦肉组(即全部组数),当针距在10cm左右时,测得直流电压更接近于0mV,有统计学意义(P<0.05)。
2.花肉组加瘦肉组(即全部组数),针距与所测得的交流电电压V关系不甚明显,有统计学意义(P<0.05)。
3.花肉组加瘦肉组(即全部组数),随着针距的增加,频率值有逐渐减少的趋势,但减少的幅度也逐渐减缓。有统计学意义(P<0.05)。花肉组结果
1.花肉组当针距在10cm左右时,测得直流电压更接近于0mV,此结论与合并数据一致,有统计学意义(P<0.05)。
2.花肉组针距与所测得的交流电压V关系不甚明显,有统计学意义(P<0.05)。
3.花肉组随着针距的增加,频率值有逐渐减少的趋势,但减少的幅度也逐渐减缓。有统计学意义(P<0.05)。
瘦肉组结果
1.瘦肉组当针距在10cm左右时,测得直流电压更接近于0mV,此结论与合并数据-致,有统计学意义(P<0.05)。
2.瘦肉组针距与所测得的交流电压V关系不甚明显,有统计学意义(P<0.05)。
3.瘦肉组随着针距的增加,频率值有逐渐减少的趋势,但减少的幅度也逐渐减缓。有统计学意义(P<0.05)。
建立两项回归方程
1.建立的回归方程为：y(测得频率)=24.247-0.756*针距cm+0.386*DCVO
2.建立的回归方程为：y(测得直流电压值)=-59.444-0.543*针距cm-0.147*DCVO+1.877*HzO
结论：
1.电针机的安全性：不管是交流电或直流电,所测出的电流单位是uA,与安全电流mA有一段差距,所测出的交流电的电压值都在2V以下,与工安的最大50V相比,有一段安全距离而直流电电压值的绝对值更仅在30mV以下,所以安全性算是不错。
2.直流电电压特殊性的探讨：当电压为0时,电位为0,两根针的中间就无电流经过(从表皮到针尖的一段深度),电流可能是经由其他电阻较少路径通过,或是使用的电表灵敏度不好,未能侦测出微弱的电流。前人的研究认为经络应该是具有电阻低、导电度佳,比较能够传递电流的特性。但出现电压为0的现象,未必代表着表皮以下有经络存在,主要是因为出现电压为0的现象都是在使用直流电之时,使用交流电则未出现此一现象,而且材料取得虽取部位接近但不一定正好都是取到同样经脉或存在经脉。同时花肉组和瘦肉组都出现“针距在lOcm左右时,测得直流电压更接近于0mV”以及“当V<0时,V的绝对值越大,针距cm越大”的结果,依据上项的结果,针距cm越大,则两针间的电阻越大,推测应该是出现了“电极化”的效应。当直流电通过人体之后,带有正、负电荷的离子在整个运动的过程中,是会遇到很大的阻力,因而造成了离子的堆积。一侧会堆积正离子,另一侧则堆积了负离子,这时就容易会出现电势差。这种电势差是和直流电流动的方向相反,所以电压呈现负值。也就是说当两根针的距离超过10cm时,很容易出现此种电压为负的现象,主要是因为距离拉长,其间的等效电阻也增大。另有一种等效阻抗的说法,人体除了一般的电阻外还有电容效应的产生,但这无法说明为何会出现电压是负值的现象。这是不是意味着电针机同对导线连接后,两端的距离是有所限制,是不能随意连接与任意拉长的,有待进一步探讨。
3.不论是瘦肉组或花肉组,其针距与所测得的交流电压V关系不甚明显,推测应该是交流电是双向的流动,所受到的电阻影响较小所致,电针机各项输出参数变动不大。
4.花肉组在测得的所有针距的直流电电压,以及大多数针距的交流电电压的平均值都大于瘦肉组,推测还是因为电阻的问题。当电流通过物体表面,为求顺利通过,必须施以合适的电压。花肉组成份上含肥肉较多,而肥肉被视为电的不良导体,因此可推测花肉组的等效电阻大于瘦肉组。电流要顺利通过花肉组表面,自然要加上比瘦肉组更多的电压。
5.花肉组在所有针距的频率,都是小于瘦肉组,也就是说,电流通过相同的距离,在花肉组表面经过的时间,要比瘦肉组还多。推测是花肉组在表面的等效电阻大于瘦肉组,因此造成频率小于瘦肉组的现象。
6.不论是花肉组或是瘦肉组,不论是在直流电还是交流电,所测量的电流(已测的部分)、电压、频率,均互不相关,没有一种连动性。插入不同花肉组或是瘦肉组的针灸针,接上电针机后,电流是向四方流动的,并不是固定向连接同对导线的方向,会有相当程度的能量消耗,造成互不相关,应该可以以此解释。
7.利用建立的回归方程为：y(测得频率)=24.247-0.756*针距cm+0.386*DCVO我们可以得到针距与频率在不同组的相关关系.两组结果,都有在一定的范围内,针距越长,频率越短的现象。
8.利用建立的回归方程为：y(测得直流电压值)=-59.444-0.543*针距cm0.147*DCVO+1.877*HzO.我们可以得到直流电电压与针距在不同组的相关关系.两组结果,总的来说都有直流电的电压越大,针距cm越小的现象。
Objective
The electroacupuncture machine of the summary is a product of the modern science and technology. A lot of emphasize in recent years the electroacupuncture machine of different treatment functions often come out. The function of electroacupuncture machine is the voltage, electric current, frequency that outputted, it add wave form on the ones different, either alter current, or direct current. Characteristic particular reached for result of treatment, being important particularly these parameters workingsed. Different frequency can influence a lot of functions of the body, like ease pain, increase different efficiency of some blood flow volume, etc., the stimuli of low frequency and high frequency will often have different treatment results, cause diversified therapeutic effects even more. Stimulation intensity to regard voltage as the main parameter, show with electric current or electric power too, take intensity which the patient can stand, this intensity is lain between while feeling threshold and threshold of pain, it will not be good to be too high or cross low curative effect. The time of electroacupuncture presents tolerance easily long, must strengthen amazing quantity again. The modulation of the wave form of the electroacupuncture machine is very important, the clinical characteristic that can be directed against disease while using, choose the appropriate wave form, it is in order to improve to treat the effect.
Superiority and specificity of the point that the electroacupuncture machine seems even more to the particular point, use electric the intersection of needle and imprison and cave Gongsun stimulate, if can effective, can make the speed that the heart beat slacken,it can actually promote the activity of the heart parasympathetic nerve, to healthy human body, to have protective regulating action. But the therapeutic function that these electroacupuncture machine emphasize, the efficiency that-judgement treatment comes with the so-called working parameter. But human itself contains the special bioelectricity, the flow of the bioelectricity can carry on different functions such as communication, the connection is in order to carry on various physiological function, to maintaining the health of the body, it is quite important. Actually human body pieces of complicated organism, contain solution, electrolytic liquid, etc. And it can carry on chemistry and physiological liquid of function in being humoral. The structure of internal tissue, cell makes up and differs, the resistance will not be the same everywhere, electric current can advance with the intersection of straight line and most short distance while being simple, power will up to from lost with the resistance, if there is any particularity, and have subject that any result emerges and worthes canvassing in two distances of acupuncture and moxibustion with a wire on earth, so design this test. This test that is one outside the human body of the purpose, is the purpose the change of the relevant parameter caused by distance between testing wire of both ends? Or seek whether to have some peculiar phenomena between two wire or not? Try to find out some common points, sum up the law, as the reference that will further tested in the future.
Methods
1. Keep depth flat over one the intersection of pig and streaky pork, electroacupuncture machine the same as to wire connect with at needle handle of two acupuncture and moxibustion, insert pig's streaky pork, the distance of two needles is lcm,2cm,4cm,6cm,8cm,10cm,12cm,14cm,16cm,18cm,20cm, use the alternating current, direct current for the power, measure its voltage and frequency value with the ammeter separately.
2. It fetch red meat of over of depths one, the others., it measure out respectively by voltage and frequency value and then.
Results
1. Streaky pork group and the red meat group(all group count). When the needle distance is about10cm, it measures voltage of the direct current approaches OmV even more, there are statistics meanings (P<0.05)
2. Streaky pork group and the red meat group(all group count). Needle distance and alternating current measured are not very obvious in voltage (V) relation, there are statistics meanings (P<0.05)
3. Streaky pork group and the red meat group(all group count). With the increasing of the needle distance, frequency value has the tendency to reduce gradually, but the reduced range is slowed down gradually. There are statistics meanings (P<0.05)
4. Streaky pork group count. When the needle distance is about10cm, it measures voltage of the direct current approaches OmV even more, there are statistics meanings (P<0.05)
5. Streaky pork group count. Needle distance and alternating current measured are not very obvious in voltage (V) relation, there are statistics meanings (P<0.05)
6. Streaky pork group count. With the increasing of the needle distance, frequency value has the tendency to reduce gradually, but the reduced range is slowed down gradually. There are statistics meanings (P<0.05) The red meat group result
7. The red meat group count. When the needle distance is about10cm, it measures voltage of the direct current approaches OmV even more, there are statistics meanings (P<0.05)
8. The red meat group count. Needle distance and alternating current measured are not very obvious in voltage (V) relation, there are statistics meanings (P<0.05)
9. The red meat group count. With the increasing of the needle distance, frequency value has the tendency to reduce gradually, but the reduced range is slowed down gradually. There are statistics meanings (P<0.05). Set up two items equation of regression
10. The equation of regression set up is:Y (measure the frequency)=24.247the distance between two needle(cm)*0.756+0.386*DCV0.
11. The equation of regression set up is:Y (measure the voltage value of direct current)=-59.444-the distance between two needle (cm)*0.543-0.147*DCV0+1.877*HzO
Conclusion
1. Security of the electroacupuncture machine:No matter alternating current or direct current, electric current units measured are uA, there is a section of disparity with the safe electric current mA, voltage values of the alternating current measured are under2V, compared with the worker safe number, highest-level50V, there is a safe distance but the absolute value of the direct current voltage value is only under30mV, so security of the electroacupuncture machine can be regarded as it is good and safe.
2. The discussion of the voltage particularity of direct current:When the voltage is0, the electric potential is close to0, there is no electric current to pass in the middle of two needles (a section of depth from epidermis to pinpoint). Perhaps the electric current is passed via less routes of the resistance of others, or the ammeter sensitivity of use is not good, fail to detect and examine the faint electric current out. Research of forefathers show collateral channels should have low resistance and have good electric conduction degree, it can relatively transmit the characteristic of the electric current. But present the voltage as the phenomenon of0, may not represent that there are collateral channels that exist under the epidermis, it is while using the direct current as the phenomenon of0mainly because the voltage appears. It use the alternating current not to present this phenomenon, and the material is obtained although fetches the position and is close to but might not be just fetched to the same passages through which vital energy circulates or passages through which vital energy circulates present. Streaky meat group and red meat group appear "the needle distance measures the direct current and presses and approaches OmV even more when about10cm" at the same time. And whether when V<0, to loud absolute value V, the needle distancr is the bigger. Result, the item in basis one result, the less large from in needle distance, the less large resistance between two stitch be, the conjecture should present electroacupuncture the Effect after the direct current passes the human body, the with, negative electric charge ion is in the course of the whole sport, will meet very great resistance, therefore cause the piling of ion. One side will pile up the cation, pile up the anion in the other side, it will easy to present the electric potential difference at this moment. Such electric potential difference is contrary to direction in which the direct current flow, so the voltage presents negative value. Or when the distance of two needles exceeds10cm, it is a negative phenomenon to present this kind of voltage very easily, mainly because the distance is elongated, the equivalent resistance during that time increases. There is statement of a kind of equivalent impedance besides, the human body has production on effect of electric capacity besides general resistance, but this unable to state why can appear voltage negative phenomenon. Mean this being electric needle machine with after not connecting wire, there are limiting to some extent on distance of both ends, it can't connect with arbitrarily elongated, remain to further canvass at will.
3. No matter the intersection of red meat and group or spend the intersection of meat and group, its needle distance and exchange not measured voltage very obvious in relation in V, the conjecture should be that the alternating current is the two-way flow, the resistance received influences smaller, every output parameter change of the electric needle machine is not big.
4. Streaky pork the intersection of meat and all the intersection of stitch and the intersection of direct current and voltage of distance that group measure, and most the intersection of stitch and the intersection of alternating current and average of voltage of distance greater than red meat group, the conjecture is still because of the question of the resistance. On the electric current through the surface of object, in order to pass smoothly, must construct with the appropriate voltage. The streaky pork group makes up and includes more fat on one, and the fat is considered as the poor conductor of the electricity, so can infer the equivalent resistance of the streaky pork group is greater than the red meat group. The electric current should add the voltage more than red meat group naturally through the streaky pork group surface of meat group smoothly.
5.The streaky pork group, in the frequency of all stitch distances, smaller than the red meat group, that is to say, the electric current, through the same distance, time to pass the colored surface of meat group is even more than red meat groups. The conjecture is that the equivalent resistance on the surface of streaky pork group is greater than the red meat group, so cause the frequency to be smaller than the phenomenon of the red meat group.
6. No matter streaky meat group or red meat group, no matter in direct current or alternating current, electric current (part already examined) measured, voltage, frequency, is each other independent, person who work up even no. Insert different colored the intersection of meat and group or the intersection of red meat and the intersection of acupuncture and moxibustion and needle of group, at connect behind the electric stitch machine, electric current to flow all sides, fixed to connect with the direction in the wire, know energy consumption to no small extent, it is independent to cause each other, can be by this explanation.
7. In the equation of regression set up is:Y (measure the frequency)=24.247-the distance between two needle (cm)*0.756+0.386*DCV0.We can get the needle distance and relevant relation in different groups of frequency. Two groups (streaky pork group and the red meat group) of results, all have within the range of suring, the longer the needle distance is, the phenomenon of the shorter frequency.
8.In the equation of regression set up is:Y (measure the voltage value of direct current)=-59.444-the distance between two needle(cm)*0.5430.147*DCV0+1.877*Hz0. We can get the voltage of direct current and relevant relation in different groups of needle distance. Two groups (streaky pork group and the red meat group) of results, the bigger the voltage that generally speaking all have the direct current is, the phenomenon that the needle is the smaller needle distance.
电针机针对特定穴位上更显得穴位的优越与特异性,例如使用电针刺激内关和公孙穴,可以更有效的抑制了心脏交感神经活性,能使心脏跳动的速率变慢,实质上可以提升心脏副交感神经的活性,对健康人体来说,具有保护性的调节作用。
然而这些电针机强调的治疗作用,仅是以所谓的工作参数来论断治疗的功效。但是人体本身就含有特殊的生物电流,这些生物电流的流动可进行沟通、联系等等不同功能以进行各种的生理作用,对维持身体的健康,相当重要。人体本来就是个复杂的有机体,体液中含有溶液、电解质液等等不同可进行化学及生理作用的液体。体内组织、细胞的结构组成不一,电阻不会是处处相同的,电流更不会单纯以直线最短的距离前进,功率更是会随距离与电阻而散失,同条导线间的两根针灸的距离内究竟有什么特殊性、以及会有什么结果产生都是值得探讨的课题,因此设计此试验。
目的：
这是一项在人体外的试验,目的在测试两端导线间是否因距离而导致相关参数的改变?或者寻求两导线间是否存在一些特异的现象?尝试找出一些共同点,归纳出规律,做为日后进一步试验的参考。
方法：
1.平放深度超过一时的猪花肉,在两根针灸针柄处连上电针机的同对导线,插入猪花肉,两根针的距离为1cm,2cm,4cm,6cm,8cm,10cm,12cm,14cm,16cm,18cm,20cm等长度,使用交流电、直流电为电源、分别以电表测量其电压与频率值。2.取深度超过一时的瘦肉,其余如方法同方法1.,再分别量出电压与频率值。
成果：
花肉组测得的直流电电压大于瘦肉组。花肉组测得的交流电电压大于瘦肉组。瘦肉组测得频率值大于花肉组。花肉组加瘦肉组(即全部组数)结果
1.花肉组加瘦肉组(即全部组数),当针距在10cm左右时,测得直流电压更接近于0mV,有统计学意义(P<0.05)。
2.花肉组加瘦肉组(即全部组数),针距与所测得的交流电电压V关系不甚明显,有统计学意义(P<0.05)。
3.花肉组加瘦肉组(即全部组数),随着针距的增加,频率值有逐渐减少的趋势,但减少的幅度也逐渐减缓。有统计学意义(P<0.05)。花肉组结果
1.花肉组当针距在10cm左右时,测得直流电压更接近于0mV,此结论与合并数据一致,有统计学意义(P<0.05)。
2.花肉组针距与所测得的交流电压V关系不甚明显,有统计学意义(P<0.05)。
3.花肉组随着针距的增加,频率值有逐渐减少的趋势,但减少的幅度也逐渐减缓。有统计学意义(P<0.05)。
瘦肉组结果
1.瘦肉组当针距在10cm左右时,测得直流电压更接近于0mV,此结论与合并数据-致,有统计学意义(P<0.05)。
2.瘦肉组针距与所测得的交流电压V关系不甚明显,有统计学意义(P<0.05)。
3.瘦肉组随着针距的增加,频率值有逐渐减少的趋势,但减少的幅度也逐渐减缓。有统计学意义(P<0.05)。
建立两项回归方程
1.建立的回归方程为：y(测得频率)=24.247-0.756*针距cm+0.386*DCVO
2.建立的回归方程为：y(测得直流电压值)=-59.444-0.543*针距cm-0.147*DCVO+1.877*HzO
结论：
1.电针机的安全性：不管是交流电或直流电,所测出的电流单位是uA,与安全电流mA有一段差距,所测出的交流电的电压值都在2V以下,与工安的最大50V相比,有一段安全距离而直流电电压值的绝对值更仅在30mV以下,所以安全性算是不错。
2.直流电电压特殊性的探讨：当电压为0时,电位为0,两根针的中间就无电流经过(从表皮到针尖的一段深度),电流可能是经由其他电阻较少路径通过,或是使用的电表灵敏度不好,未能侦测出微弱的电流。前人的研究认为经络应该是具有电阻低、导电度佳,比较能够传递电流的特性。但出现电压为0的现象,未必代表着表皮以下有经络存在,主要是因为出现电压为0的现象都是在使用直流电之时,使用交流电则未出现此一现象,而且材料取得虽取部位接近但不一定正好都是取到同样经脉或存在经脉。同时花肉组和瘦肉组都出现“针距在lOcm左右时,测得直流电压更接近于0mV”以及“当V<0时,V的绝对值越大,针距cm越大”的结果,依据上项的结果,针距cm越大,则两针间的电阻越大,推测应该是出现了“电极化”的效应。当直流电通过人体之后,带有正、负电荷的离子在整个运动的过程中,是会遇到很大的阻力,因而造成了离子的堆积。一侧会堆积正离子,另一侧则堆积了负离子,这时就容易会出现电势差。这种电势差是和直流电流动的方向相反,所以电压呈现负值。也就是说当两根针的距离超过10cm时,很容易出现此种电压为负的现象,主要是因为距离拉长,其间的等效电阻也增大。另有一种等效阻抗的说法,人体除了一般的电阻外还有电容效应的产生,但这无法说明为何会出现电压是负值的现象。这是不是意味着电针机同对导线连接后,两端的距离是有所限制,是不能随意连接与任意拉长的,有待进一步探讨。
3.不论是瘦肉组或花肉组,其针距与所测得的交流电压V关系不甚明显,推测应该是交流电是双向的流动,所受到的电阻影响较小所致,电针机各项输出参数变动不大。
4.花肉组在测得的所有针距的直流电电压,以及大多数针距的交流电电压的平均值都大于瘦肉组,推测还是因为电阻的问题。当电流通过物体表面,为求顺利通过,必须施以合适的电压。花肉组成份上含肥肉较多,而肥肉被视为电的不良导体,因此可推测花肉组的等效电阻大于瘦肉组。电流要顺利通过花肉组表面,自然要加上比瘦肉组更多的电压。
5.花肉组在所有针距的频率,都是小于瘦肉组,也就是说,电流通过相同的距离,在花肉组表面经过的时间,要比瘦肉组还多。推测是花肉组在表面的等效电阻大于瘦肉组,因此造成频率小于瘦肉组的现象。
6.不论是花肉组或是瘦肉组,不论是在直流电还是交流电,所测量的电流(已测的部分)、电压、频率,均互不相关,没有一种连动性。插入不同花肉组或是瘦肉组的针灸针,接上电针机后,电流是向四方流动的,并不是固定向连接同对导线的方向,会有相当程度的能量消耗,造成互不相关,应该可以以此解释。
7.利用建立的回归方程为：y(测得频率)=24.247-0.756*针距cm+0.386*DCVO我们可以得到针距与频率在不同组的相关关系.两组结果,都有在一定的范围内,针距越长,频率越短的现象。
8.利用建立的回归方程为：y(测得直流电压值)=-59.444-0.543*针距cm0.147*DCVO+1.877*HzO.我们可以得到直流电电压与针距在不同组的相关关系.两组结果,总的来说都有直流电的电压越大,针距cm越小的现象。
Objective
The electroacupuncture machine of the summary is a product of the modern science and technology. A lot of emphasize in recent years the electroacupuncture machine of different treatment functions often come out. The function of electroacupuncture machine is the voltage, electric current, frequency that outputted, it add wave form on the ones different, either alter current, or direct current. Characteristic particular reached for result of treatment, being important particularly these parameters workingsed. Different frequency can influence a lot of functions of the body, like ease pain, increase different efficiency of some blood flow volume, etc., the stimuli of low frequency and high frequency will often have different treatment results, cause diversified therapeutic effects even more. Stimulation intensity to regard voltage as the main parameter, show with electric current or electric power too, take intensity which the patient can stand, this intensity is lain between while feeling threshold and threshold of pain, it will not be good to be too high or cross low curative effect. The time of electroacupuncture presents tolerance easily long, must strengthen amazing quantity again. The modulation of the wave form of the electroacupuncture machine is very important, the clinical characteristic that can be directed against disease while using, choose the appropriate wave form, it is in order to improve to treat the effect.
Superiority and specificity of the point that the electroacupuncture machine seems even more to the particular point, use electric the intersection of needle and imprison and cave Gongsun stimulate, if can effective, can make the speed that the heart beat slacken,it can actually promote the activity of the heart parasympathetic nerve, to healthy human body, to have protective regulating action. But the therapeutic function that these electroacupuncture machine emphasize, the efficiency that-judgement treatment comes with the so-called working parameter. But human itself contains the special bioelectricity, the flow of the bioelectricity can carry on different functions such as communication, the connection is in order to carry on various physiological function, to maintaining the health of the body, it is quite important. Actually human body pieces of complicated organism, contain solution, electrolytic liquid, etc. And it can carry on chemistry and physiological liquid of function in being humoral. The structure of internal tissue, cell makes up and differs, the resistance will not be the same everywhere, electric current can advance with the intersection of straight line and most short distance while being simple, power will up to from lost with the resistance, if there is any particularity, and have subject that any result emerges and worthes canvassing in two distances of acupuncture and moxibustion with a wire on earth, so design this test. This test that is one outside the human body of the purpose, is the purpose the change of the relevant parameter caused by distance between testing wire of both ends? Or seek whether to have some peculiar phenomena between two wire or not? Try to find out some common points, sum up the law, as the reference that will further tested in the future.
Methods
1. Keep depth flat over one the intersection of pig and streaky pork, electroacupuncture machine the same as to wire connect with at needle handle of two acupuncture and moxibustion, insert pig's streaky pork, the distance of two needles is lcm,2cm,4cm,6cm,8cm,10cm,12cm,14cm,16cm,18cm,20cm, use the alternating current, direct current for the power, measure its voltage and frequency value with the ammeter separately.
2. It fetch red meat of over of depths one, the others., it measure out respectively by voltage and frequency value and then.
Results
1. Streaky pork group and the red meat group(all group count). When the needle distance is about10cm, it measures voltage of the direct current approaches OmV even more, there are statistics meanings (P<0.05)
2. Streaky pork group and the red meat group(all group count). Needle distance and alternating current measured are not very obvious in voltage (V) relation, there are statistics meanings (P<0.05)
3. Streaky pork group and the red meat group(all group count). With the increasing of the needle distance, frequency value has the tendency to reduce gradually, but the reduced range is slowed down gradually. There are statistics meanings (P<0.05)
4. Streaky pork group count. When the needle distance is about10cm, it measures voltage of the direct current approaches OmV even more, there are statistics meanings (P<0.05)
5. Streaky pork group count. Needle distance and alternating current measured are not very obvious in voltage (V) relation, there are statistics meanings (P<0.05)
6. Streaky pork group count. With the increasing of the needle distance, frequency value has the tendency to reduce gradually, but the reduced range is slowed down gradually. There are statistics meanings (P<0.05) The red meat group result
7. The red meat group count. When the needle distance is about10cm, it measures voltage of the direct current approaches OmV even more, there are statistics meanings (P<0.05)
8. The red meat group count. Needle distance and alternating current measured are not very obvious in voltage (V) relation, there are statistics meanings (P<0.05)
9. The red meat group count. With the increasing of the needle distance, frequency value has the tendency to reduce gradually, but the reduced range is slowed down gradually. There are statistics meanings (P<0.05). Set up two items equation of regression
10. The equation of regression set up is:Y (measure the frequency)=24.247the distance between two needle(cm)*0.756+0.386*DCV0.
11. The equation of regression set up is:Y (measure the voltage value of direct current)=-59.444-the distance between two needle (cm)*0.543-0.147*DCV0+1.877*HzO
Conclusion
1. Security of the electroacupuncture machine:No matter alternating current or direct current, electric current units measured are uA, there is a section of disparity with the safe electric current mA, voltage values of the alternating current measured are under2V, compared with the worker safe number, highest-level50V, there is a safe distance but the absolute value of the direct current voltage value is only under30mV, so security of the electroacupuncture machine can be regarded as it is good and safe.
2. The discussion of the voltage particularity of direct current:When the voltage is0, the electric potential is close to0, there is no electric current to pass in the middle of two needles (a section of depth from epidermis to pinpoint). Perhaps the electric current is passed via less routes of the resistance of others, or the ammeter sensitivity of use is not good, fail to detect and examine the faint electric current out. Research of forefathers show collateral channels should have low resistance and have good electric conduction degree, it can relatively transmit the characteristic of the electric current. But present the voltage as the phenomenon of0, may not represent that there are collateral channels that exist under the epidermis, it is while using the direct current as the phenomenon of0mainly because the voltage appears. It use the alternating current not to present this phenomenon, and the material is obtained although fetches the position and is close to but might not be just fetched to the same passages through which vital energy circulates or passages through which vital energy circulates present. Streaky meat group and red meat group appear "the needle distance measures the direct current and presses and approaches OmV even more when about10cm" at the same time. And whether when V<0, to loud absolute value V, the needle distancr is the bigger. Result, the item in basis one result, the less large from in needle distance, the less large resistance between two stitch be, the conjecture should present electroacupuncture the Effect after the direct current passes the human body, the with, negative electric charge ion is in the course of the whole sport, will meet very great resistance, therefore cause the piling of ion. One side will pile up the cation, pile up the anion in the other side, it will easy to present the electric potential difference at this moment. Such electric potential difference is contrary to direction in which the direct current flow, so the voltage presents negative value. Or when the distance of two needles exceeds10cm, it is a negative phenomenon to present this kind of voltage very easily, mainly because the distance is elongated, the equivalent resistance during that time increases. There is statement of a kind of equivalent impedance besides, the human body has production on effect of electric capacity besides general resistance, but this unable to state why can appear voltage negative phenomenon. Mean this being electric needle machine with after not connecting wire, there are limiting to some extent on distance of both ends, it can't connect with arbitrarily elongated, remain to further canvass at will.
3. No matter the intersection of red meat and group or spend the intersection of meat and group, its needle distance and exchange not measured voltage very obvious in relation in V, the conjecture should be that the alternating current is the two-way flow, the resistance received influences smaller, every output parameter change of the electric needle machine is not big.
4. Streaky pork the intersection of meat and all the intersection of stitch and the intersection of direct current and voltage of distance that group measure, and most the intersection of stitch and the intersection of alternating current and average of voltage of distance greater than red meat group, the conjecture is still because of the question of the resistance. On the electric current through the surface of object, in order to pass smoothly, must construct with the appropriate voltage. The streaky pork group makes up and includes more fat on one, and the fat is considered as the poor conductor of the electricity, so can infer the equivalent resistance of the streaky pork group is greater than the red meat group. The electric current should add the voltage more than red meat group naturally through the streaky pork group surface of meat group smoothly.
5.The streaky pork group, in the frequency of all stitch distances, smaller than the red meat group, that is to say, the electric current, through the same distance, time to pass the colored surface of meat group is even more than red meat groups. The conjecture is that the equivalent resistance on the surface of streaky pork group is greater than the red meat group, so cause the frequency to be smaller than the phenomenon of the red meat group.
6. No matter streaky meat group or red meat group, no matter in direct current or alternating current, electric current (part already examined) measured, voltage, frequency, is each other independent, person who work up even no. Insert different colored the intersection of meat and group or the intersection of red meat and the intersection of acupuncture and moxibustion and needle of group, at connect behind the electric stitch machine, electric current to flow all sides, fixed to connect with the direction in the wire, know energy consumption to no small extent, it is independent to cause each other, can be by this explanation.
7. In the equation of regression set up is:Y (measure the frequency)=24.247-the distance between two needle (cm)*0.756+0.386*DCV0.We can get the needle distance and relevant relation in different groups of frequency. Two groups (streaky pork group and the red meat group) of results, all have within the range of suring, the longer the needle distance is, the phenomenon of the shorter frequency.
8.In the equation of regression set up is:Y (measure the voltage value of direct current)=-59.444-the distance between two needle(cm)*0.5430.147*DCV0+1.877*Hz0. We can get the voltage of direct current and relevant relation in different groups of needle distance. Two groups (streaky pork group and the red meat group) of results, the bigger the voltage that generally speaking all have the direct current is, the phenomenon that the needle is the smaller needle distance.
引文
[1]周幸来,周幸秋,孙冰.电针疗法大全.湖南：湖南科学技术出版社.2010.
[2]石学敏,针灸学,中国中医药出版社,北京：2002,8：170-172.
[3]洪文学,刘海燕.电针镇痛的原理及仪器.医疗卫生装备.2006.
[4]林海波.电针疗法.北京：中国医药科技出版社.2012.9
[5]刘立安,戴淑青.临床电针疗法.北京：中国医药科技出版社.2011.
[6]佟玉杰.现代针灸电子仪器及其应用.哈尔滨：黑龙江科学技术出版社.1984.
[7]马宁.浅谈电流效应对人体的影响.科技向导.2013,6：174,190.
[8]龚贤平.脉冲式电疗治疗糖尿病中风偏瘫痪者的疗效观察及护理[J].齐齐哈尔医学院学报.2013,34(2)：293-294.
[9]杨群.周围神经损伤的电刺激疗法.现代康复.2000：11.
[10]董子平.电针治疗抑郁症101例Chinese Acupuncture & Maxibustion.2010,1(21).
[11]陈子瑜,李万瑶.电针机疗法的应用.中国针灸学会针灸器材专业委员会第十四届学术研讨会参会代表手册.2011,41-45.
[12]王玉国,康山林,赵宝群.大学物理学(下册).北京：科学出版社.2013.1.
[13]陈珺.人体的电流效应[J].卫生职业教育.2011.29(4)：158-159.
[14]黄海滨,任超世.生物电阻抗分析法(BIA)测量人体成分.国外医学生物医学工程分册.2000.23(3)：151-154.
[15]袁国祥.2006。低频电流的人体效应研究与应用。硕士论文。湖北：华中师范大学无线电物理研究所.
[16]李梅伍,陈雪芹,杨月芬.物理教师.1998,(3)19：29.
[17]张三慧.大学物理学—基于相对论的电磁学.北京：清华大学出版社.2008.9.
[18]郑利岩,王巍,张江艳.于经络输入不同频率的声波调节人体虚实证的探索[J].针灸临床杂志,1998,14(1)：7-9.
[19]刘言寿.试论电针疗法临床补泻的模拟运用[J].针灸临床杂志,1997,13(4、5)：3-4.
[20]范军铭,焦伟,赵京伟等.变频电针仪治疗痛证的临床疗效观察[J].上海针灸杂志,1999,18(2)：9-11.
[21]王延武.不同频率电针效应的实验和临床研究[J].安徽中医学院学报,2006,25(3)：58-60.
[22]陈嵘,赵京伟,田中华.电针仪频率的改变对虚实痛证的疗效观察[J].中医研究,2001,14(3)：58-59.
[23]曹炀,徐爱民,邢鲁栋等.针刺手法电针仪的创新原理及其特点.首届国际针灸器材学术研讨会论文集,2009：58-68.
[24]Wall Brain. The gate control theory of pain mechanisms:A re-examination and re-statement,1978.101:1-18.
[25]P. D. Wall. Future trends in pain research, Phil. Trans. R. Soc. B.1985.308(1136):393-401.
[26]武晓东,王佩.针刺镇痛机理研究概述[J].中国中医基础医学杂志,1999,5(2).
[27]牛汉璋,江赛男,韩太真,等.穴位背根电位的传入神经纤维,陕西新医药,1983(12)6：52-53.
[28]Lin JG, Chen XH, Han JS. Antinociception produced by 2 and 5Hz peripheral stimulation in the rat. Intern J Neuroscience.1992;64:15-22.
[29]Tsai HY, Lin JG, Inoki R. Further evidence for possible analgesic mechanism of electroacupuncture:effect on neuropeptides and serotonergic neurons in rat spinal cord. Japan Journal of Pharmacology.1989;49:181-185.
[30]Tsai HY, Chen YF, Lin JG. etc. Effects of electroacupuncture analgesia on the central monoaminergic neurons. Chin Pharma J.1994;46:207-218.
[31]曹永昌.2003。电针刺激与一氧化氮合成酵素抑制剂结合的镇痛研究—以福尔马林疼痛测试与Fos蛋白测量之大白鼠模式。博士论文。台中：中国医药学院中西医结合研究所.
[32]周飞,李福凤,程介士,等.电针对急性脑缺血大鼠局部脑血流和脑梗死体积的影响[J].上海针灸杂志,2003,22(5)：3-6.
[33]周飞,郭景春,程介士,等.频率相关的电针抗急性脑缺血大鼠的效应的观察.中国针灸学会第七届全国中青年针灸推拿学术研讨会论文汇编.2006：96-99.
[34]韩济生.针刺镇痛频率特异性的进一步证明[J].针刺研究,2001,26(3)：923.
[35]王京友,王双坤.不同强度和频率电针的镇痛效应[J].针刺研究,1993,18(1)：44.
[36]孙瑞卿,王贺春,王韵等.不同频率的电针对大鼠神经源性痛的治疗作用[J].中国应用生理学杂志,2002,18(2)：128.
[37]邹燃,张红星,张唐法等.不同频率电针与药物治疗急性痛风性关节炎的比较[J].中国临床康复,2006,10(43)：188-189.
[38]朱丽霞,李文武,吉长福等.不同强度电针对痛觉异常治疗作用的比较[J].中国疼痛医学杂志,1996：2：26.
[39]]Richard S. S. Cheng, Bruce Pomeranz:Electroacupuncture analgesia could be mediated by at least two pain-relieving mechanisms:endorphin and non-endorphin systems. Life Sci.1979;25:1957-1962.
[40]Han JS, Ding XZ, Fan XG. The frequency as the cardinal determinant for electroacupuncture analgesia to be reverse by opioid antagonists. Acta Physiol. Sin. 1986,38(5):475-482.
[41]Chen XH, Han JS. Analgesia induced by electroacupuncture of different frequencies is mediated by different types of opiod receptors. Another cross-tolerance study. Behav. Brain Res.1992;47:143-149.
[42]Sun SL, Han JS. High and low frequency electroacupuncture analgesia are mediated by different types of opiod receptors at spinal level. A cross-tolerance study. Acta. Physiol. Sin.1989;41(4):416-420.
[43]Chen XH, Han JS. All three types of opioid receptors in the spinal cord are important for 2/15 Hz electroacupuncture analgesia. Eur. J. Pharmacol.1992;211:203-210.
[44]魏以斌.2001。不同频率电针三阴交穴(SP-6)对剖腹产术后疼痛效应之评估。硕士论文。台中：中国医药学院中国医学研究所.
[45]高忠汉.2002。电针止痛效果与大白鼠脊髓背角神经中细胞致癌基因c-fos之关联。硕士论文。台中：中国医药学院中西医结合研究所
[46]陈海林,王全权.不同频率的穴位电刺激对急性软组织损伤疼痛的影响[J].成都中医药大学学报.2006,22(6)：357-358.
[47]吴炫璋.2007。电针刺激内关与公孙穴对健康人心脏自主神经功能与胃电图之影响。硕士论文。花莲：慈济大学整合生理暨临床科学研究所.
[48]Wang Q. Mao LM, Han JS. The arcuate nucleus of hypothalamus mediates low but not high frequency electroacupuncture analgesia in rats. Brain Res.1990;513:60-66.
[49]Wang Q. Mao LM, Han JS. Analgesia electric stimulation of the hypothalamic arcuate nucleus:tolerance and its cross tolerance to 2Hz or100Hz electroacupuncture. Brain Res.1990;518:40-46.
[50]Lee JH, Beitz AJ. Electroacupuncture modifies the expression of c-fos in the spinal cord induced by noxious stimulation. Brain Res.1992,557:80-91.
[51]卢峻,时宇静,金智秀等.不同频率电针对模型大鼠抗抑郁效应的比较研究[J].北京中医药大学学报,2003,26(6)：83.
[52]焦杨,王焕梅.不同频率的电针治疗腰椎间盘突出症的疗效观察.中国针灸学会2009年学术年会论文集,2009：807-809.
[53]Han JS. Acupuncture:neuropeptide release produced by electrical stimulation of different frequencies. Trends Neurosci.2003,26 (1):17-22.
[54]Wu MT, Sheen JM, Chuang KH, Yang P, Chin SL, Tsai CY, Chen CJ, Liao JR, Lai PH, Chu KA, Pan HB, Yang CF. Neuroanal Specificity of acupuncture respond:a fMRI study with electroacupuncture. Neuroimage.2002:16:1028-1037.
[55]康玉典.2007。比较电针和经皮电刺激两侧足三里穴对正中神经体性感觉诱发电位的影响。硕士论文。台中：中国医药学院针灸研究所.
[56]Pomeranz B, Warma N. Electroacupuncture suppression of a nociceptive reflex is potentiated by two repeated electroacupuncture treatments:The first opioid effect potentiates a second non-opioid effect. Brain. Res.1988,452:232-236.
[57]Pomeranz B, Bibic L. Electroacupuncture suppresses a nociceptive reflex:naltrexone prevents but does not reverse this effect. Brain Res.1988,452:27-231.
[58]Wang Y, Wang S. Analgesic effects of electroacupuncture stimulation at different intensities and frtquencies. Chen Tzu Ten Chiu.1993,18(1):44-47.
[59]顾光,金祖培,周逸平等.电排针治疗小儿麻痹后遗症1076例临床疗效总结[J].安徽中医学院学报,1988,7(2)：40-42.
[60]汪润生,刑江淮PCE—88A型程控电针仪治疗172例神经系统疾病的临床观察[J].针刺研究,1994,19(2)：11-13.
[61]Han JS, Ding SZ, Fan SG. Cholecystokinin octapeptide(CCK8):antagonism to electroacupuncture analgesia and a possible role in electroacupuncture tolerance. Pain.1986,27:101-115.
[62]彭至贤.2003。电针穴位对膝关节镜手术术后先机止痛之疗效评估。硕士论文。台中：中国医药学院中西医结合研究所.
[63]顾新建,刘维平,邢江淮等PCE-A型过程控制电针仪的研制[J],中国医疗器械杂志,19943(18)：146-148.
[64]David Jonathan Grant, Jane Bishop-Miller, David Magnus Winchester.etc. A randommizedcomparative trial of acupuncture versus transcutaneous electricalnerve stimulation for chronic back pain in the elderly. Pain.1999,82:9-13.
[65]Hamza Mohamed A, White Paul F, Ahmed Hesham E,etc. Effect of the frequency of transcutaneous electrical nerve stimulation on the postoperative opioid analgesic requirement and recovery profile. Anesthesiology.1999,91(5).
[66]翁清松,杨哲彦、谢文祥,多频电刺激于腰痛症之临床治疗评估,2002年工程科技与中西医学应用研讨会,2002：163-165.
[67]施议强.2006。电针人体中脘穴对血糖与胰岛素值的影响。硕士论文。花莲：南华大学自然医学研究所.
[68]东贵荣,东红升,斐浩.音乐电针对大鼠脑内CCK, mRNA表达影响的对比研究[J].针灸临床杂志.2003,21(3)：58-60.
[69]施雪筠.生理学[M].北京：中国中医药出版社,2003：346-348.
[70]曾家顺.2003。由电针刺激大白鼠俞穴释出调控神经化学物质之探讨。硕士论文。台中：中国医药学院中西医结合研究所.
[71]Zytkowski A. Ectodermal method of Ryodorak —an attempt at clinical measurement for evaluation of physiotherapy effects in patients with low back pain. Neurol Neurochir Pol.1999,32(6):207-215.
[72]Irnich W. Advisory information from Vitatron. Pacing Clin Electrophysiol. 2002,25(1):128.
[73]王世军.电针对大鼠海马CA1区微循环血流量的影响[J].中国针灸.2005,12(9)5：308-310.
[74]Lin JG, Chang SL, Cheng JT. Release of β-endorphin from adrenal gland to lower plasma glucose by the electroacupuncture at Zhongwan acupoint in rats. Nerosci Lett. 2002,326:17-20.
[75]Chang SL, Lin JG, Chi TC.etc. An insulin-dependent hypoglycaemia induced by electroacupuncture at the Zhongwan(CV12) acupoint in diabetic rats. Diabetologia. 1999,42:250-255.
[76]张光远.1996。低、高频率电针刺激足三里穴对人体生理功能之影响：脉搏、血压、皮温、腋温。硕士论文。台中：中国医药学院中国医学研究所.
[77]周仁伟.1999。高、低频率电针刺激手、足三里穴对人体胃电图、胃肠道激素和血糖之影响。硕士论文。台中：中国医药学院中国医学研究所.
[78]嵇波,李晓泓,丁娜等.急性心肌缺血损伤后大鼠经穴NE含量的变化及不同频率电针干预作用.首届皇甫谧故里拜祖大典暨《针灸甲乙经》学术思想国际研讨会论文集,2012：135.
[79]嵇波,胡琅琳,李晓泓等.急性心肌缺血损伤后大鼠经穴CaM含量的变化及不同频率电针干预作用[J].北京中医药大学学报,2012,35(6)：422-425.
[80]嵇波,胡琅琳,李晓泓等.急性心肌缺血损伤后大鼠穴区NO和NOS的变化及不同频率电针的干预作用[J].北京中医药大学学报,2012,35(8)：563-566.
[81]黄学勇,李佩芳.针刺督脉配合经皮电刺激在脑卒中偏瘫康复中的应用[J].中医药临床杂志,2009,21(3)：222-223.
[82]胡永善.下腰痛的生物力学特点及康复[J].颈腰痛杂志,2004,25(2)：73-75.
[83]贡瑾,曹智刚.头部穴区经皮神经功能电刺激治疗脑卒中后抑郁[J].实用医学杂志,2906,22(19)：2256-2257.
[84]陈景藻.现代物理治疗学.北京：人民军医出版社,2001：109-114;333-336.
[85]朱美玲,赵皿,刘洪臣TENS镇痛及影响因素[J].中华老年口腔医学杂志,2004,2(3)：187-189.
[86]周和平,胡庆祥.经皮神经电刺激治疗周围神经痛机制探讨[J].中国康复,1989;4：71.
[87]金真,张蔚婷,罗非等.人脑对不同频率穴位电刺激反应的功能性磁共振成像[J].生理学报,2001,53(4)：275-280.
[88]蒋袁絮,王韵,刘红香等.2Hz和100Hz经皮神经电刺激对大鼠慢性炎症痛治疗作用的比较[J].中国中西医结合杂志,2001,21(12)：923-925.
[89]Han JS, Chen XH, Sun SI. Effect of low and high frequency TENS on Met-enkephalin-Arg-Phe and dynophin A immunoreactivity in human lumbar CSF. Pain,1991,47:295-298.
[90]Dubuisson D. Effect of dorsal-column stimulation on gelatinosa and marginal neurons of cat spinal cord. Journal of Neuro-sufery,1989,70:257-265.
[91]Romita VV, Yashpal K, Hui-Chan CWY, et al. Intense peripheral electrical stimulation evokes brief and persistent inhibition of the nociceptive tail withdraw reflex in the rat. Brain Research.1997,761:192-202.
[92]郝银菊,蒋袁絮TENS对关节炎大鼠脊髓三种神经肽含量的影响[J].南京医科大学学报(自然科学版).2008,28(2)：170-172.
[93]王晓宇,尚红燕,何伟等.不同频率和持续时间经皮电刺激耳甲区对大鼠癫痫发作抑制作用的比较[J].针刺研究.2012,37(6)：447-457.
[94]方剑乔,马桂芝,梁宜.不同时间经皮穴位电刺激抗大鼠慢性运动性疲劳以及对海马和下丘脑形态与5-羟色胺表达的影响[J].上海针灸杂志.2010,29(11)：735-738.
[95]Kerns JM, Freeman JA. Effects of D. C. electrical stimulation on nerve regeneration in the rat sciatic nerve. SOC Neurosci Abstr.1986,11:7.
[96]米立新,王彦香,张仲等.经皮电神经刺激对周围神经再生的影响[J].中华物理医学与康复杂志.2003,25(4)：209-211.
[97]白玉龙,胡永善,林伟平等.阴极经皮电刺激促进周围神经功能恢复的电生理学研究[J].中国康复医学杂志.2005,20(5)：329-330.
[98]白玉龙,胡永善,林伟平等.定量评估经皮神经电刺激促进周围神经的再生和功能恢复[J].中国康复医学杂志.2005,21(9)：49-51.
[99]吴鎏桢,阎俊娟,韩济生.2/100 Hz经皮穴位电刺激对27例青少年网络成瘾症的治疗作用[J].中国药物依赖性杂志,2007,16(1)：32-35.
[100]闫俊娟,倪牧宇,曲之毅等.2/100 Hz经皮穴位电刺激治疗青少年网瘾ET结果的初步探讨[J].中国药物依赖性杂志,2008,17(4)：282-285.
[101]权志鹏,秦明星,韩济生等.2/100 Hz经皮穴位电刺激对社区戒毒康复中阿片成瘾者的作用[J].中国药物依赖性杂志,2008,17(4)：282-285.
[102]Han JS, Wang Q. M obilization of specific neuropeptides by peripheral stimulation of identified frequencies. News Physiol Sci,1992,7:176-180.
[103]Han JS, Zhang RL. Suppression of morphine abstinence syndrome by body electroacupuncture of diifferent frequencies in rats. Drug Alcohol Depend, 1993,31:169-175.
[104]吴鎏桢,崔彩莲,韩济生.2/100 Hz经皮穴位电刺激可降低脱毒期美沙酮用量和脱毒后近期抑郁及焦虑情绪[J].中国药物依赖性杂志,2001,10(2)：124-126
[105]吴鎏桢,崔彩莲,韩济生.2/100 Hz跨皮肤电刺激对男性海洛因成瘾者性机能障碍及血清性激素水平的影响[J].中国中西医结合杂志,2000,20(1)：15-33
[106]张本国,罗非,刘崇悦等.单独应用韩氏戒毒仪治疗海洛因成瘾121例报告[J].中国中西医结合杂志,2000,20(8)：593-595.
[107]迟戈,马艳彬,李非等.直流电疗法的临床作用[J].中国医疗器械信息.2010.16(10)：42-44.
[108]胡鸿志.新型低频电脉冲理疗仪的研制.电子测量技术.2011.34(7)：5-7.
[109]孙彦奇,徐琦民.绝缘电针疗法[J].中国针灸.2002：182-183.
[110]赵秋生.从人体电流效应解读人体触电的安全阈值[J].安全科学技术.2012,5：10-13.
[111]GB/T 13870-1992.电流通过人体的效应,1992.
[112]商振德.经络的本质-人体生物电循环系统[J].中国针灸.2011.31(3)：277-280.
[113]卓廉士,李学智.经脉感应论[J].中国针灸.2004.24(9)：631-633.
[114]盛国俊,董永贵,董恩生等.人体生物电阻抗的脉冲式检测方法及其应用[J].仪器仪表学报.2008.29(4)：295-298.
[115]刘堂义,杨华元,蒯乐等.单穴电针仪的研制及其意义[J].辽宁中医杂志,2010,37(2)：241-243.
[2]石学敏,针灸学,中国中医药出版社,北京：2002,8：170-172.
[3]洪文学,刘海燕.电针镇痛的原理及仪器.医疗卫生装备.2006.
[4]林海波.电针疗法.北京：中国医药科技出版社.2012.9
[5]刘立安,戴淑青.临床电针疗法.北京：中国医药科技出版社.2011.
[6]佟玉杰.现代针灸电子仪器及其应用.哈尔滨：黑龙江科学技术出版社.1984.
[7]马宁.浅谈电流效应对人体的影响.科技向导.2013,6：174,190.
[8]龚贤平.脉冲式电疗治疗糖尿病中风偏瘫痪者的疗效观察及护理[J].齐齐哈尔医学院学报.2013,34(2)：293-294.
[9]杨群.周围神经损伤的电刺激疗法.现代康复.2000：11.
[10]董子平.电针治疗抑郁症101例Chinese Acupuncture & Maxibustion.2010,1(21).
[11]陈子瑜,李万瑶.电针机疗法的应用.中国针灸学会针灸器材专业委员会第十四届学术研讨会参会代表手册.2011,41-45.
[12]王玉国,康山林,赵宝群.大学物理学(下册).北京：科学出版社.2013.1.
[13]陈珺.人体的电流效应[J].卫生职业教育.2011.29(4)：158-159.
[14]黄海滨,任超世.生物电阻抗分析法(BIA)测量人体成分.国外医学生物医学工程分册.2000.23(3)：151-154.
[15]袁国祥.2006。低频电流的人体效应研究与应用。硕士论文。湖北：华中师范大学无线电物理研究所.
[16]李梅伍,陈雪芹,杨月芬.物理教师.1998,(3)19：29.
[17]张三慧.大学物理学—基于相对论的电磁学.北京：清华大学出版社.2008.9.
[18]郑利岩,王巍,张江艳.于经络输入不同频率的声波调节人体虚实证的探索[J].针灸临床杂志,1998,14(1)：7-9.
[19]刘言寿.试论电针疗法临床补泻的模拟运用[J].针灸临床杂志,1997,13(4、5)：3-4.
[20]范军铭,焦伟,赵京伟等.变频电针仪治疗痛证的临床疗效观察[J].上海针灸杂志,1999,18(2)：9-11.
[21]王延武.不同频率电针效应的实验和临床研究[J].安徽中医学院学报,2006,25(3)：58-60.
[22]陈嵘,赵京伟,田中华.电针仪频率的改变对虚实痛证的疗效观察[J].中医研究,2001,14(3)：58-59.
[23]曹炀,徐爱民,邢鲁栋等.针刺手法电针仪的创新原理及其特点.首届国际针灸器材学术研讨会论文集,2009：58-68.
[24]Wall Brain. The gate control theory of pain mechanisms:A re-examination and re-statement,1978.101:1-18.
[25]P. D. Wall. Future trends in pain research, Phil. Trans. R. Soc. B.1985.308(1136):393-401.
[26]武晓东,王佩.针刺镇痛机理研究概述[J].中国中医基础医学杂志,1999,5(2).
[27]牛汉璋,江赛男,韩太真,等.穴位背根电位的传入神经纤维,陕西新医药,1983(12)6：52-53.
[28]Lin JG, Chen XH, Han JS. Antinociception produced by 2 and 5Hz peripheral stimulation in the rat. Intern J Neuroscience.1992;64:15-22.
[29]Tsai HY, Lin JG, Inoki R. Further evidence for possible analgesic mechanism of electroacupuncture:effect on neuropeptides and serotonergic neurons in rat spinal cord. Japan Journal of Pharmacology.1989;49:181-185.
[30]Tsai HY, Chen YF, Lin JG. etc. Effects of electroacupuncture analgesia on the central monoaminergic neurons. Chin Pharma J.1994;46:207-218.
[31]曹永昌.2003。电针刺激与一氧化氮合成酵素抑制剂结合的镇痛研究—以福尔马林疼痛测试与Fos蛋白测量之大白鼠模式。博士论文。台中：中国医药学院中西医结合研究所.
[32]周飞,李福凤,程介士,等.电针对急性脑缺血大鼠局部脑血流和脑梗死体积的影响[J].上海针灸杂志,2003,22(5)：3-6.
[33]周飞,郭景春,程介士,等.频率相关的电针抗急性脑缺血大鼠的效应的观察.中国针灸学会第七届全国中青年针灸推拿学术研讨会论文汇编.2006：96-99.
[34]韩济生.针刺镇痛频率特异性的进一步证明[J].针刺研究,2001,26(3)：923.
[35]王京友,王双坤.不同强度和频率电针的镇痛效应[J].针刺研究,1993,18(1)：44.
[36]孙瑞卿,王贺春,王韵等.不同频率的电针对大鼠神经源性痛的治疗作用[J].中国应用生理学杂志,2002,18(2)：128.
[37]邹燃,张红星,张唐法等.不同频率电针与药物治疗急性痛风性关节炎的比较[J].中国临床康复,2006,10(43)：188-189.
[38]朱丽霞,李文武,吉长福等.不同强度电针对痛觉异常治疗作用的比较[J].中国疼痛医学杂志,1996：2：26.
[39]]Richard S. S. Cheng, Bruce Pomeranz:Electroacupuncture analgesia could be mediated by at least two pain-relieving mechanisms:endorphin and non-endorphin systems. Life Sci.1979;25:1957-1962.
[40]Han JS, Ding XZ, Fan XG. The frequency as the cardinal determinant for electroacupuncture analgesia to be reverse by opioid antagonists. Acta Physiol. Sin. 1986,38(5):475-482.
[41]Chen XH, Han JS. Analgesia induced by electroacupuncture of different frequencies is mediated by different types of opiod receptors. Another cross-tolerance study. Behav. Brain Res.1992;47:143-149.
[42]Sun SL, Han JS. High and low frequency electroacupuncture analgesia are mediated by different types of opiod receptors at spinal level. A cross-tolerance study. Acta. Physiol. Sin.1989;41(4):416-420.
[43]Chen XH, Han JS. All three types of opioid receptors in the spinal cord are important for 2/15 Hz electroacupuncture analgesia. Eur. J. Pharmacol.1992;211:203-210.
[44]魏以斌.2001。不同频率电针三阴交穴(SP-6)对剖腹产术后疼痛效应之评估。硕士论文。台中：中国医药学院中国医学研究所.
[45]高忠汉.2002。电针止痛效果与大白鼠脊髓背角神经中细胞致癌基因c-fos之关联。硕士论文。台中：中国医药学院中西医结合研究所
[46]陈海林,王全权.不同频率的穴位电刺激对急性软组织损伤疼痛的影响[J].成都中医药大学学报.2006,22(6)：357-358.
[47]吴炫璋.2007。电针刺激内关与公孙穴对健康人心脏自主神经功能与胃电图之影响。硕士论文。花莲：慈济大学整合生理暨临床科学研究所.
[48]Wang Q. Mao LM, Han JS. The arcuate nucleus of hypothalamus mediates low but not high frequency electroacupuncture analgesia in rats. Brain Res.1990;513:60-66.
[49]Wang Q. Mao LM, Han JS. Analgesia electric stimulation of the hypothalamic arcuate nucleus:tolerance and its cross tolerance to 2Hz or100Hz electroacupuncture. Brain Res.1990;518:40-46.
[50]Lee JH, Beitz AJ. Electroacupuncture modifies the expression of c-fos in the spinal cord induced by noxious stimulation. Brain Res.1992,557:80-91.
[51]卢峻,时宇静,金智秀等.不同频率电针对模型大鼠抗抑郁效应的比较研究[J].北京中医药大学学报,2003,26(6)：83.
[52]焦杨,王焕梅.不同频率的电针治疗腰椎间盘突出症的疗效观察.中国针灸学会2009年学术年会论文集,2009：807-809.
[53]Han JS. Acupuncture:neuropeptide release produced by electrical stimulation of different frequencies. Trends Neurosci.2003,26 (1):17-22.
[54]Wu MT, Sheen JM, Chuang KH, Yang P, Chin SL, Tsai CY, Chen CJ, Liao JR, Lai PH, Chu KA, Pan HB, Yang CF. Neuroanal Specificity of acupuncture respond:a fMRI study with electroacupuncture. Neuroimage.2002:16:1028-1037.
[55]康玉典.2007。比较电针和经皮电刺激两侧足三里穴对正中神经体性感觉诱发电位的影响。硕士论文。台中：中国医药学院针灸研究所.
[56]Pomeranz B, Warma N. Electroacupuncture suppression of a nociceptive reflex is potentiated by two repeated electroacupuncture treatments:The first opioid effect potentiates a second non-opioid effect. Brain. Res.1988,452:232-236.
[57]Pomeranz B, Bibic L. Electroacupuncture suppresses a nociceptive reflex:naltrexone prevents but does not reverse this effect. Brain Res.1988,452:27-231.
[58]Wang Y, Wang S. Analgesic effects of electroacupuncture stimulation at different intensities and frtquencies. Chen Tzu Ten Chiu.1993,18(1):44-47.
[59]顾光,金祖培,周逸平等.电排针治疗小儿麻痹后遗症1076例临床疗效总结[J].安徽中医学院学报,1988,7(2)：40-42.
[60]汪润生,刑江淮PCE—88A型程控电针仪治疗172例神经系统疾病的临床观察[J].针刺研究,1994,19(2)：11-13.
[61]Han JS, Ding SZ, Fan SG. Cholecystokinin octapeptide(CCK8):antagonism to electroacupuncture analgesia and a possible role in electroacupuncture tolerance. Pain.1986,27:101-115.
[62]彭至贤.2003。电针穴位对膝关节镜手术术后先机止痛之疗效评估。硕士论文。台中：中国医药学院中西医结合研究所.
[63]顾新建,刘维平,邢江淮等PCE-A型过程控制电针仪的研制[J],中国医疗器械杂志,19943(18)：146-148.
[64]David Jonathan Grant, Jane Bishop-Miller, David Magnus Winchester.etc. A randommizedcomparative trial of acupuncture versus transcutaneous electricalnerve stimulation for chronic back pain in the elderly. Pain.1999,82:9-13.
[65]Hamza Mohamed A, White Paul F, Ahmed Hesham E,etc. Effect of the frequency of transcutaneous electrical nerve stimulation on the postoperative opioid analgesic requirement and recovery profile. Anesthesiology.1999,91(5).
[66]翁清松,杨哲彦、谢文祥,多频电刺激于腰痛症之临床治疗评估,2002年工程科技与中西医学应用研讨会,2002：163-165.
[67]施议强.2006。电针人体中脘穴对血糖与胰岛素值的影响。硕士论文。花莲：南华大学自然医学研究所.
[68]东贵荣,东红升,斐浩.音乐电针对大鼠脑内CCK, mRNA表达影响的对比研究[J].针灸临床杂志.2003,21(3)：58-60.
[69]施雪筠.生理学[M].北京：中国中医药出版社,2003：346-348.
[70]曾家顺.2003。由电针刺激大白鼠俞穴释出调控神经化学物质之探讨。硕士论文。台中：中国医药学院中西医结合研究所.
[71]Zytkowski A. Ectodermal method of Ryodorak —an attempt at clinical measurement for evaluation of physiotherapy effects in patients with low back pain. Neurol Neurochir Pol.1999,32(6):207-215.
[72]Irnich W. Advisory information from Vitatron. Pacing Clin Electrophysiol. 2002,25(1):128.
[73]王世军.电针对大鼠海马CA1区微循环血流量的影响[J].中国针灸.2005,12(9)5：308-310.
[74]Lin JG, Chang SL, Cheng JT. Release of β-endorphin from adrenal gland to lower plasma glucose by the electroacupuncture at Zhongwan acupoint in rats. Nerosci Lett. 2002,326:17-20.
[75]Chang SL, Lin JG, Chi TC.etc. An insulin-dependent hypoglycaemia induced by electroacupuncture at the Zhongwan(CV12) acupoint in diabetic rats. Diabetologia. 1999,42:250-255.
[76]张光远.1996。低、高频率电针刺激足三里穴对人体生理功能之影响：脉搏、血压、皮温、腋温。硕士论文。台中：中国医药学院中国医学研究所.
[77]周仁伟.1999。高、低频率电针刺激手、足三里穴对人体胃电图、胃肠道激素和血糖之影响。硕士论文。台中：中国医药学院中国医学研究所.
[78]嵇波,李晓泓,丁娜等.急性心肌缺血损伤后大鼠经穴NE含量的变化及不同频率电针干预作用.首届皇甫谧故里拜祖大典暨《针灸甲乙经》学术思想国际研讨会论文集,2012：135.
[79]嵇波,胡琅琳,李晓泓等.急性心肌缺血损伤后大鼠经穴CaM含量的变化及不同频率电针干预作用[J].北京中医药大学学报,2012,35(6)：422-425.
[80]嵇波,胡琅琳,李晓泓等.急性心肌缺血损伤后大鼠穴区NO和NOS的变化及不同频率电针的干预作用[J].北京中医药大学学报,2012,35(8)：563-566.
[81]黄学勇,李佩芳.针刺督脉配合经皮电刺激在脑卒中偏瘫康复中的应用[J].中医药临床杂志,2009,21(3)：222-223.
[82]胡永善.下腰痛的生物力学特点及康复[J].颈腰痛杂志,2004,25(2)：73-75.
[83]贡瑾,曹智刚.头部穴区经皮神经功能电刺激治疗脑卒中后抑郁[J].实用医学杂志,2906,22(19)：2256-2257.
[84]陈景藻.现代物理治疗学.北京：人民军医出版社,2001：109-114;333-336.
[85]朱美玲,赵皿,刘洪臣TENS镇痛及影响因素[J].中华老年口腔医学杂志,2004,2(3)：187-189.
[86]周和平,胡庆祥.经皮神经电刺激治疗周围神经痛机制探讨[J].中国康复,1989;4：71.
[87]金真,张蔚婷,罗非等.人脑对不同频率穴位电刺激反应的功能性磁共振成像[J].生理学报,2001,53(4)：275-280.
[88]蒋袁絮,王韵,刘红香等.2Hz和100Hz经皮神经电刺激对大鼠慢性炎症痛治疗作用的比较[J].中国中西医结合杂志,2001,21(12)：923-925.
[89]Han JS, Chen XH, Sun SI. Effect of low and high frequency TENS on Met-enkephalin-Arg-Phe and dynophin A immunoreactivity in human lumbar CSF. Pain,1991,47:295-298.
[90]Dubuisson D. Effect of dorsal-column stimulation on gelatinosa and marginal neurons of cat spinal cord. Journal of Neuro-sufery,1989,70:257-265.
[91]Romita VV, Yashpal K, Hui-Chan CWY, et al. Intense peripheral electrical stimulation evokes brief and persistent inhibition of the nociceptive tail withdraw reflex in the rat. Brain Research.1997,761:192-202.
[92]郝银菊,蒋袁絮TENS对关节炎大鼠脊髓三种神经肽含量的影响[J].南京医科大学学报(自然科学版).2008,28(2)：170-172.
[93]王晓宇,尚红燕,何伟等.不同频率和持续时间经皮电刺激耳甲区对大鼠癫痫发作抑制作用的比较[J].针刺研究.2012,37(6)：447-457.
[94]方剑乔,马桂芝,梁宜.不同时间经皮穴位电刺激抗大鼠慢性运动性疲劳以及对海马和下丘脑形态与5-羟色胺表达的影响[J].上海针灸杂志.2010,29(11)：735-738.
[95]Kerns JM, Freeman JA. Effects of D. C. electrical stimulation on nerve regeneration in the rat sciatic nerve. SOC Neurosci Abstr.1986,11:7.
[96]米立新,王彦香,张仲等.经皮电神经刺激对周围神经再生的影响[J].中华物理医学与康复杂志.2003,25(4)：209-211.
[97]白玉龙,胡永善,林伟平等.阴极经皮电刺激促进周围神经功能恢复的电生理学研究[J].中国康复医学杂志.2005,20(5)：329-330.
[98]白玉龙,胡永善,林伟平等.定量评估经皮神经电刺激促进周围神经的再生和功能恢复[J].中国康复医学杂志.2005,21(9)：49-51.
[99]吴鎏桢,阎俊娟,韩济生.2/100 Hz经皮穴位电刺激对27例青少年网络成瘾症的治疗作用[J].中国药物依赖性杂志,2007,16(1)：32-35.
[100]闫俊娟,倪牧宇,曲之毅等.2/100 Hz经皮穴位电刺激治疗青少年网瘾ET结果的初步探讨[J].中国药物依赖性杂志,2008,17(4)：282-285.
[101]权志鹏,秦明星,韩济生等.2/100 Hz经皮穴位电刺激对社区戒毒康复中阿片成瘾者的作用[J].中国药物依赖性杂志,2008,17(4)：282-285.
[102]Han JS, Wang Q. M obilization of specific neuropeptides by peripheral stimulation of identified frequencies. News Physiol Sci,1992,7:176-180.
[103]Han JS, Zhang RL. Suppression of morphine abstinence syndrome by body electroacupuncture of diifferent frequencies in rats. Drug Alcohol Depend, 1993,31:169-175.
[104]吴鎏桢,崔彩莲,韩济生.2/100 Hz经皮穴位电刺激可降低脱毒期美沙酮用量和脱毒后近期抑郁及焦虑情绪[J].中国药物依赖性杂志,2001,10(2)：124-126
[105]吴鎏桢,崔彩莲,韩济生.2/100 Hz跨皮肤电刺激对男性海洛因成瘾者性机能障碍及血清性激素水平的影响[J].中国中西医结合杂志,2000,20(1)：15-33
[106]张本国,罗非,刘崇悦等.单独应用韩氏戒毒仪治疗海洛因成瘾121例报告[J].中国中西医结合杂志,2000,20(8)：593-595.
[107]迟戈,马艳彬,李非等.直流电疗法的临床作用[J].中国医疗器械信息.2010.16(10)：42-44.
[108]胡鸿志.新型低频电脉冲理疗仪的研制.电子测量技术.2011.34(7)：5-7.
[109]孙彦奇,徐琦民.绝缘电针疗法[J].中国针灸.2002：182-183.
[110]赵秋生.从人体电流效应解读人体触电的安全阈值[J].安全科学技术.2012,5：10-13.
[111]GB/T 13870-1992.电流通过人体的效应,1992.
[112]商振德.经络的本质-人体生物电循环系统[J].中国针灸.2011.31(3)：277-280.
[113]卓廉士,李学智.经脉感应论[J].中国针灸.2004.24(9)：631-633.
[114]盛国俊,董永贵,董恩生等.人体生物电阻抗的脉冲式检测方法及其应用[J].仪器仪表学报.2008.29(4)：295-298.
[115]刘堂义,杨华元,蒯乐等.单穴电针仪的研制及其意义[J].辽宁中医杂志,2010,37(2)：241-243.