苦参内生拮抗细菌筛选及其抗菌物质纯化鉴定
|
摘要
该论文从中药材苦参中分离筛选具有抑菌活性的内生细菌,对其分类地位、发酵条件、定殖能力、抗菌物质纯化鉴定及其机用制进行了系统研究。分离筛选获得了具有广谱抑菌作用的内生细菌B_(36)菌株,鉴定为枯草芽孢杆菌;明确了B_(36)菌株的最佳发酵条件;从B_(36)菌株发酵液中分离纯化出了2种抗菌物质,经鉴定为环状脂肽类的抗生素;该抗生素可破坏番茄叶霉病菌细胞膜,并能够抑制菌丝生长和孢子萌发。该研究对植物内生细菌的开发与应用和新型微生物农药的创制,具有重要理论指导意义及一定实际应用价值。
从苦参根中分离筛选到了拮抗内生细菌B_(36)菌株。采用稀释平板法从辽宁省沈阳天柱山上采集的苦参根内一共分离到了148株内生细菌,通过平板对峙培养法得到29株具有较强活性的菌株,再进一步通过对菌株无菌滤液抑菌活性的测定和盆栽活体试验筛选得到了抑菌活性强的目的生防菌B_(36)菌株。该菌株的无菌滤液对玉米弯孢霉叶斑病菌[Curvularia lunata(Walk)Boed]、菜豆炭疽病菌(Colletotrichum lindemuthianum)、小麦根腐病菌(Helminthosporium sorokinianum Pam.et al.)、番茄灰霉病菌(Botrytis cinereaPers.)、番茄叶霉病菌[Fulvia fulva(Cooke) Cifferri]等病原菌表现出了很强的抑制作用,抑菌圈直径都在25.0mm以上,对小麦白粉病的离体叶片的防效达到了95.71%,而盆栽防效也很高达83.20%,对番茄灰霉病也有一定的预防效果(防效为71.49%)。因此该菌株作为生防菌具有一定的研究和应用潜力。
对B_(36)菌株的生理生化性质进行研究,结合分子生物学手段确定了分类地位。B_(36)菌株性质为革兰氏阳性反应,有芽孢,可运动,利用柠檬酸盐,利用葡萄糖,蔗糖,果糖,甘露醇产酸,可还原硝酸盐为亚硝酸盐,水解淀粉和明胶,利用葡萄糖发酵产酸等。其16SrDNA全序列共有1463bp,通过建立系统发育树得出枯草芽孢杆菌Bacillus subtilisstrain B432与菌株B_(36)的亲缘关系最近,聚在一起。因此鉴定B_(36)菌株为枯草芽孢杆菌(Bacillus subtilis)。
通过单因子试验和正交试验方法,得到了最适枯草芽孢杆菌B_(36)菌株的发酵培养条件。最适宜发酵条件为发酵温度28-33℃,转速150 r·min~(-1),接种量20%,初始pH 9.0,在250mL三角瓶的装液量为50 mL,发酵36h。最佳培养基组成为葡萄糖2.0%,酵母粉0.5%,大豆粉0.5%,NH_4NO_3 0.5%,CaCO_3 1.0%。优化培养条件后B_(36)菌株产生抗菌物质能力明显增强,抑菌圈直径提高了13.3%。
采用抗生素双标记的方法研究了枯草芽孢杆菌B_(36)菌株在番茄植株体内的定殖动态。结果表明,接种方法显著影响B_(36)菌株在植株体内的定殖和传导,以浸根、浸种处理效果最好,灌根次之,喷雾和针刺处理在植株根部检测不到B_(36)菌株。采用浸根法接种后结果表明标记菌株可在番茄根际和根表有效定殖,在根际定殖能力强于根表,定殖动态为先向下降后上升再下降。浸根处理后标记菌株能在番茄体内定殖,并传导至茎部和叶片,随着接种天数的增加,标记菌株在根部定殖数量整体上呈下降趋势,而在茎部和叶部定殖的菌量均呈现出“先增后降”的趋势,到后期各组织中菌量基本趋于稳定。
采用有机溶剂沉淀法,溶媒萃取法和大孔吸附树脂法对枯草芽孢杆菌B_(36)菌株发酵产生的抑菌活性成分进行初步提取。结果表明,有机溶剂沉淀法和溶媒萃取法提取抗菌成分的能力较低,而大孔吸附树脂法提取抗菌成分能力强。因此选择大孔树脂HP20进行吸附,以75%乙醇为洗脱剂进行动态解吸初步提取枯草芽孢杆菌B_(36)菌株的抗菌成分。
采用硅胶柱层析,结合薄层层析和生物活性追踪方法分离枯草芽孢杆菌B_(36)代谢产物的粗提物,经过3次硅胶柱层析后得到活性最好,含量最高的组分。通过HPLC-MS分析,负离子电离后得到洗脱时间分别为5.7min和6.9min的两种化合物,进ESI\MS二级质谱电离后得到其结构。分子量为1042.5Da的化合物A结构为Cys-Asp-Tyr-Gln-氨基脂肪酸-Pro-Asn-Gln,7环肽C_(14)的环状脂肽类化合物;分子量为1056.5Da的化合物B结构为氨基脂肪酸-Asn-Asp-Met-Gln-Tyr-Asn-Gln-Asn-Pro-Ser,10环肽C_(17)的环状脂肽类化合物。
采用生物化学等方法研究枯草芽孢杆菌B_(36)菌株抗菌物质对番茄叶霉病菌的作用机制。结果表明,菌株B_(36)抗菌物质粗提物对番茄叶霉病菌抑菌圈直径达20.5mm,并引起菌丝畸形,产生囊泡,并抑制分生孢子萌发,当抗菌物质浓度为1.06×10~3μg·mL~(-1)时,孢子萌发抑制率达到86.56%,果胶酶和羧甲基纤维素酶的酶活分别为0.5911 U·mL~(-1)和0.01757 U·mL~(-1),显著低于对照,说明B_(36)抗菌物质抑制两种菌丝胞外细胞壁水解酶的合成,并通过破坏细胞膜结构产生抑菌作用,而抗菌物质对病菌菌丝细胞壁组成物质几丁质和菌丝蛋白质的浓度没有影响。
An endophytic bacterium with antagonistic activity was isolated from Sophora which belonged to traditional Chinese medicines and its classification,ferment conditions, colonizing ability,and its extraction method and antifungal mechanism to pathogen of its antagonistic substances were studied.Strain B_(36) was abtained which was Bacillus subtilis and it could colonize effectively on Figs.Two antagonistic substances of endophytic bacteria from Sophora were the first time obtained,and it belonged to cyclic lipopeptide,which destroyed the structure of plasma membrane and expressed obvious inhibition on spores germination and grown on hyphae of Fulvia fulva.
Strain B_(36) was obtained from Sophora flavescens Ait root.148 bacteria strains were isolated by dilution- Fig method from roots of Sophora flavescens Ait were sampled at Tianzhu mountain in Shenyang City Liaoning Province.29bacteria strains showed antagonistic activity though confront culture method against pathogens on Fig,then the aimed strain B_(36) which sterile-filtered supernatant had the strongest antagonistic activity against pathogens in Fig experiment as well as in pot culture trails was screened.Strain B_(36) had antagonistic effect on Helminthosporium sativu Pammel.el al.,Curvularia lunata (Wakker) Boed,Colletotrichum lindemuthianum,Botrytis cinerea Pers.,and Fulvia fulva (Cooke) Cifferris et al.,the inhibition zone diameters were all arrived at 25.0mm.As well as strain B36 showed biological control activity against Wheat powdery mildew of experiment in vitro and in pot and against tomato gray mold with control effect were 95.71%,83.20% and 71.49%respectively,so Strain B_(36) had great capacity of research and application.
According to the characteristics of morphological feature,physiology,biochemistry tests and the comparison of 16SrRNA sequence the classification of strain B_(36) was established. The results indicated that it was positive of gram,germ staining and acid production from glucose,and utilized glucose,sucrose,mannitol,fructose as carbon source,it could reduce nitrate,hydrolysis starch and gelatin.The16S rDNA sequence of B_(36) Strain was 1463bp, according to the phylogenetic tree,closest relative strain to B_(36) Strain was Bacillus subtilis B432,as the strain B_(36) was Bacillus subtilis.
The culture conditions and the compositions of cultural medium of Endophytic B36 strain which was isolated from Sophora with Antifungal Activity were studied by using the single factor and orthogonal design method.According to the antagonistic activity of sterile-filtered B36 and its grown.The optimal fermentation parameters were 28-33℃,150 r·min-1,pH 9.0,20%inoculation volume,and 50mL culture in 250mL flask for 36h,which had the strongest antagonistic activity.The fermentation medium was modified and the optimized medium was composed of sucrose 2.0%,yeast 0.5%,soybean flour 0.5%, NH_4NO_30.5%and CaCO_31.0%.Under this optimized condition,the inhibition zone diameter increased 13.3%to the initial condition.
Colonizing dynamics of t he strain B_(36) in tomato were studied by labeling method with double antibiotics-resistance.The results showed that strain B_(36) marked with double antibiotics-resistance could colonize the root of tomato and deliver into the issues which were influenced significantly by inoculation methods.The density of marked strain in the tomato after inoculated by pruned root and soaking seed were munch more then by soil drench,there were no marked strain in root of tomato by spraying foliar and daubing leaf method.After inoculated with pruned root,the marked strain B_(36) could colonize the root of tomato that could enhance the biocontrol effect to pantheons,and the strain B_(36) had stronger colonize ability around the roots then in the surface of the roots.Then the marked strain could deliver into internal stem and leaf tissues,and its density decreased in root gradually while increased in stem and foliage then decreased as the plant growing.
The antifungal substances of Baciliius subtilis B_(36) strain were extracted by organic solvents precipitates,solvents extraction and macroporous adsorption resin methods.The extraction effect of macroporous adsorption resin method had showed better effection than the other methods.So macroporous resin HP20 was adopted to adsorb the antifungal substances of Baciliius subtilis B_(36) and 75%ethanol was selected for desorption the antifungal substances that with dynamic experiment.
A component had greatest antagonistic activity and content was obtained by three times separated with silica gel column,then analyzed by HPLC-MS negative ion.Two components with elution time were 5.7min and 6.9min respectively were obtained,and their composition were determined by electro spray ionization mass spectrometry.The results showed that component A(with a molecular weight of 1042.5Da) had a primary structure of seven cyclic lipopetides link to C_(14):Cys-Asp-Tyr-Gln-Fatty acid-Pro-Asn-Gln,and component B(with a molecular weight of 1056.5Da) had a primary structure of ten cyclic link to C_(17):Fatty acid-Asn -Asp-Met-Gln-Tyr-Asn-Gln-Asn-Pro-Ser.
Antifungal effect of active substances extracted by macroporous adsorption resin method from endophytic bacteria B_(36) to Fulvia fulva(Cooke)Cif.were studied.The results showed that the pathogenic hyphae grew in abnormal shape and produced sac,and the inhibition zone diameter was 20.5mm after treated with the crude extracts of antifungal substances.The antifungal substances also expressed obvious inhibition effect on spores germination.The inhibition rate was 86.56%and the activity of PMG and Cx were 0.5911 U·mL~(-1) and 0.01757 U·mL~(-1) which were both lower than the control significantly when the concentration of antifungal substances arrived at 1.06×10~3μg·mL~(-1).So it was concluded that the antifungal substances could inhibit the activity of PMG and Cx and destroy the structure of plasma membrane,but could not decompose the chitin or inhibit the content of proteins.
从苦参根中分离筛选到了拮抗内生细菌B_(36)菌株。采用稀释平板法从辽宁省沈阳天柱山上采集的苦参根内一共分离到了148株内生细菌,通过平板对峙培养法得到29株具有较强活性的菌株,再进一步通过对菌株无菌滤液抑菌活性的测定和盆栽活体试验筛选得到了抑菌活性强的目的生防菌B_(36)菌株。该菌株的无菌滤液对玉米弯孢霉叶斑病菌[Curvularia lunata(Walk)Boed]、菜豆炭疽病菌(Colletotrichum lindemuthianum)、小麦根腐病菌(Helminthosporium sorokinianum Pam.et al.)、番茄灰霉病菌(Botrytis cinereaPers.)、番茄叶霉病菌[Fulvia fulva(Cooke) Cifferri]等病原菌表现出了很强的抑制作用,抑菌圈直径都在25.0mm以上,对小麦白粉病的离体叶片的防效达到了95.71%,而盆栽防效也很高达83.20%,对番茄灰霉病也有一定的预防效果(防效为71.49%)。因此该菌株作为生防菌具有一定的研究和应用潜力。
对B_(36)菌株的生理生化性质进行研究,结合分子生物学手段确定了分类地位。B_(36)菌株性质为革兰氏阳性反应,有芽孢,可运动,利用柠檬酸盐,利用葡萄糖,蔗糖,果糖,甘露醇产酸,可还原硝酸盐为亚硝酸盐,水解淀粉和明胶,利用葡萄糖发酵产酸等。其16SrDNA全序列共有1463bp,通过建立系统发育树得出枯草芽孢杆菌Bacillus subtilisstrain B432与菌株B_(36)的亲缘关系最近,聚在一起。因此鉴定B_(36)菌株为枯草芽孢杆菌(Bacillus subtilis)。
通过单因子试验和正交试验方法,得到了最适枯草芽孢杆菌B_(36)菌株的发酵培养条件。最适宜发酵条件为发酵温度28-33℃,转速150 r·min~(-1),接种量20%,初始pH 9.0,在250mL三角瓶的装液量为50 mL,发酵36h。最佳培养基组成为葡萄糖2.0%,酵母粉0.5%,大豆粉0.5%,NH_4NO_3 0.5%,CaCO_3 1.0%。优化培养条件后B_(36)菌株产生抗菌物质能力明显增强,抑菌圈直径提高了13.3%。
采用抗生素双标记的方法研究了枯草芽孢杆菌B_(36)菌株在番茄植株体内的定殖动态。结果表明,接种方法显著影响B_(36)菌株在植株体内的定殖和传导,以浸根、浸种处理效果最好,灌根次之,喷雾和针刺处理在植株根部检测不到B_(36)菌株。采用浸根法接种后结果表明标记菌株可在番茄根际和根表有效定殖,在根际定殖能力强于根表,定殖动态为先向下降后上升再下降。浸根处理后标记菌株能在番茄体内定殖,并传导至茎部和叶片,随着接种天数的增加,标记菌株在根部定殖数量整体上呈下降趋势,而在茎部和叶部定殖的菌量均呈现出“先增后降”的趋势,到后期各组织中菌量基本趋于稳定。
采用有机溶剂沉淀法,溶媒萃取法和大孔吸附树脂法对枯草芽孢杆菌B_(36)菌株发酵产生的抑菌活性成分进行初步提取。结果表明,有机溶剂沉淀法和溶媒萃取法提取抗菌成分的能力较低,而大孔吸附树脂法提取抗菌成分能力强。因此选择大孔树脂HP20进行吸附,以75%乙醇为洗脱剂进行动态解吸初步提取枯草芽孢杆菌B_(36)菌株的抗菌成分。
采用硅胶柱层析,结合薄层层析和生物活性追踪方法分离枯草芽孢杆菌B_(36)代谢产物的粗提物,经过3次硅胶柱层析后得到活性最好,含量最高的组分。通过HPLC-MS分析,负离子电离后得到洗脱时间分别为5.7min和6.9min的两种化合物,进ESI\MS二级质谱电离后得到其结构。分子量为1042.5Da的化合物A结构为Cys-Asp-Tyr-Gln-氨基脂肪酸-Pro-Asn-Gln,7环肽C_(14)的环状脂肽类化合物;分子量为1056.5Da的化合物B结构为氨基脂肪酸-Asn-Asp-Met-Gln-Tyr-Asn-Gln-Asn-Pro-Ser,10环肽C_(17)的环状脂肽类化合物。
采用生物化学等方法研究枯草芽孢杆菌B_(36)菌株抗菌物质对番茄叶霉病菌的作用机制。结果表明,菌株B_(36)抗菌物质粗提物对番茄叶霉病菌抑菌圈直径达20.5mm,并引起菌丝畸形,产生囊泡,并抑制分生孢子萌发,当抗菌物质浓度为1.06×10~3μg·mL~(-1)时,孢子萌发抑制率达到86.56%,果胶酶和羧甲基纤维素酶的酶活分别为0.5911 U·mL~(-1)和0.01757 U·mL~(-1),显著低于对照,说明B_(36)抗菌物质抑制两种菌丝胞外细胞壁水解酶的合成,并通过破坏细胞膜结构产生抑菌作用,而抗菌物质对病菌菌丝细胞壁组成物质几丁质和菌丝蛋白质的浓度没有影响。
An endophytic bacterium with antagonistic activity was isolated from Sophora which belonged to traditional Chinese medicines and its classification,ferment conditions, colonizing ability,and its extraction method and antifungal mechanism to pathogen of its antagonistic substances were studied.Strain B_(36) was abtained which was Bacillus subtilis and it could colonize effectively on Figs.Two antagonistic substances of endophytic bacteria from Sophora were the first time obtained,and it belonged to cyclic lipopeptide,which destroyed the structure of plasma membrane and expressed obvious inhibition on spores germination and grown on hyphae of Fulvia fulva.
Strain B_(36) was obtained from Sophora flavescens Ait root.148 bacteria strains were isolated by dilution- Fig method from roots of Sophora flavescens Ait were sampled at Tianzhu mountain in Shenyang City Liaoning Province.29bacteria strains showed antagonistic activity though confront culture method against pathogens on Fig,then the aimed strain B_(36) which sterile-filtered supernatant had the strongest antagonistic activity against pathogens in Fig experiment as well as in pot culture trails was screened.Strain B_(36) had antagonistic effect on Helminthosporium sativu Pammel.el al.,Curvularia lunata (Wakker) Boed,Colletotrichum lindemuthianum,Botrytis cinerea Pers.,and Fulvia fulva (Cooke) Cifferris et al.,the inhibition zone diameters were all arrived at 25.0mm.As well as strain B36 showed biological control activity against Wheat powdery mildew of experiment in vitro and in pot and against tomato gray mold with control effect were 95.71%,83.20% and 71.49%respectively,so Strain B_(36) had great capacity of research and application.
According to the characteristics of morphological feature,physiology,biochemistry tests and the comparison of 16SrRNA sequence the classification of strain B_(36) was established. The results indicated that it was positive of gram,germ staining and acid production from glucose,and utilized glucose,sucrose,mannitol,fructose as carbon source,it could reduce nitrate,hydrolysis starch and gelatin.The16S rDNA sequence of B_(36) Strain was 1463bp, according to the phylogenetic tree,closest relative strain to B_(36) Strain was Bacillus subtilis B432,as the strain B_(36) was Bacillus subtilis.
The culture conditions and the compositions of cultural medium of Endophytic B36 strain which was isolated from Sophora with Antifungal Activity were studied by using the single factor and orthogonal design method.According to the antagonistic activity of sterile-filtered B36 and its grown.The optimal fermentation parameters were 28-33℃,150 r·min-1,pH 9.0,20%inoculation volume,and 50mL culture in 250mL flask for 36h,which had the strongest antagonistic activity.The fermentation medium was modified and the optimized medium was composed of sucrose 2.0%,yeast 0.5%,soybean flour 0.5%, NH_4NO_30.5%and CaCO_31.0%.Under this optimized condition,the inhibition zone diameter increased 13.3%to the initial condition.
Colonizing dynamics of t he strain B_(36) in tomato were studied by labeling method with double antibiotics-resistance.The results showed that strain B_(36) marked with double antibiotics-resistance could colonize the root of tomato and deliver into the issues which were influenced significantly by inoculation methods.The density of marked strain in the tomato after inoculated by pruned root and soaking seed were munch more then by soil drench,there were no marked strain in root of tomato by spraying foliar and daubing leaf method.After inoculated with pruned root,the marked strain B_(36) could colonize the root of tomato that could enhance the biocontrol effect to pantheons,and the strain B_(36) had stronger colonize ability around the roots then in the surface of the roots.Then the marked strain could deliver into internal stem and leaf tissues,and its density decreased in root gradually while increased in stem and foliage then decreased as the plant growing.
The antifungal substances of Baciliius subtilis B_(36) strain were extracted by organic solvents precipitates,solvents extraction and macroporous adsorption resin methods.The extraction effect of macroporous adsorption resin method had showed better effection than the other methods.So macroporous resin HP20 was adopted to adsorb the antifungal substances of Baciliius subtilis B_(36) and 75%ethanol was selected for desorption the antifungal substances that with dynamic experiment.
A component had greatest antagonistic activity and content was obtained by three times separated with silica gel column,then analyzed by HPLC-MS negative ion.Two components with elution time were 5.7min and 6.9min respectively were obtained,and their composition were determined by electro spray ionization mass spectrometry.The results showed that component A(with a molecular weight of 1042.5Da) had a primary structure of seven cyclic lipopetides link to C_(14):Cys-Asp-Tyr-Gln-Fatty acid-Pro-Asn-Gln,and component B(with a molecular weight of 1056.5Da) had a primary structure of ten cyclic link to C_(17):Fatty acid-Asn -Asp-Met-Gln-Tyr-Asn-Gln-Asn-Pro-Ser.
Antifungal effect of active substances extracted by macroporous adsorption resin method from endophytic bacteria B_(36) to Fulvia fulva(Cooke)Cif.were studied.The results showed that the pathogenic hyphae grew in abnormal shape and produced sac,and the inhibition zone diameter was 20.5mm after treated with the crude extracts of antifungal substances.The antifungal substances also expressed obvious inhibition effect on spores germination.The inhibition rate was 86.56%and the activity of PMG and Cx were 0.5911 U·mL~(-1) and 0.01757 U·mL~(-1) which were both lower than the control significantly when the concentration of antifungal substances arrived at 1.06×10~3μg·mL~(-1).So it was concluded that the antifungal substances could inhibit the activity of PMG and Cx and destroy the structure of plasma membrane,but could not decompose the chitin or inhibit the content of proteins.
引文
1.陈贵斌,牛晋阳,张建勇等.反相高效液相色谱法测定抗生素AGPM发酵液中的主要成分.色谱,2003,21(4):378-381.
2.陈丽梅,樊妙姬,李玲.甘蔗固氮营养醋杆菌的研究进展.微生物学通报,2000,27(1):63-66.
3.陈文君,吴剑波,全启光.农用抗生素11371各组分的分离纯化和鉴别.中国抗生素杂志,1990,15(1):42-48.
4.陈志谊,刘永锋,陆凡.水稻纹枯病生防菌Bs-916产业化生产关键技术.植物保护学报,2004,31(3):230-233.
5.陈志谊,陆凡,刘春祥,等.水稻纹枯病拮抗细菌B-916培养条件与发酵配方的研究.西北农业学报.1998,12(1):76-8.
6.陈志谊,许志刚,陆凡,等.拮抗细菌B-916对水稻植株的抗性诱导作用.2001,14(2):44-48.
7.崔云龙,刘训理.枯草芽饱杆菌B-921菌株对数种植物病原真菌的抗生作用.生物防治通报,1993,9(1):29-31.
8.崔宗均,苏宝森,蔡妙英,等.北方水稻田固氮细菌资源的研究(简报).中国农业大学学报,1996,1(3):64-80.
9.习亚梅,粟韩,李捷,等.小麦白粉病离体叶段筛选方法初探.浙江化工,2000,31卷增刊:78-80.
10.东秀珠,蔡妙英.常见细菌系统鉴定手册.北京:科学出版社,2001.
11.董合忠,李维江.植物诱导抗病性及其利用.莱阳农学院学报,2001,18(4):268-269.
12.樊连梅.诺尔斯链霉菌西昌变种发酵液宁南霉素分离纯化工艺及其性质研究.沈阳农业大学硕士学位论文,2000.6.
13.方中达,植病研究方法.2004,中国农业出版社.
14.傅正擎,夏正俊,吴蔼民,等.内生菌对棉花黄萎病病菌及毒素的抑制作用和对棉花的促生作用.植物病理学,1999,29(4):374-375.
15.傅正挚等.内生菌对棉花黄萎病病菌及毒素的抑制作用和对棉花的促生作用.植物病理学报,1999,29(4):374-375.
16.高学文,姚仕义,HuongPham,等.枯草芽孢杆菌B2菌株产生的抑菌活性物质分析.中国生物防治,2003,19(3):175-179.
17.高学文,姚仕义,Pham Uong,Vater Joachim,等.基因工程菌枯草芽孢杆菌GEB3产生的脂肽类抗生素及生物活性研究.中国农业大学学报,2003,36(12):1496-1501.
18.高学文,姚仕义,Pham Uong,Vater Joachim,等.枯草芽孢杆菌B2菌株产生的表面活性素变异体的纯化和鉴定.微生物学报,2003,45(5):647-652.
19.高增贵,2007,玉米内生细菌种群多样性与纹枯病生防机理的研究.沈阳农业大学博士学位论文.
20.高增贵,陈捷,刘军华,等.拮抗内生细菌B202006菌株对玉米主要防御酶系的影响.植物病理学报,2007,37(1):102-104.
21.辜运富,张云飞,张小平.一株抗玉米纹枯病内生细菌的分离鉴定及其抗病促生作用.微生物学报,2008,35(8):1240-1245.
22.谷祖敏.2008草茎点霉作为生物除草剂防除鸭跖草的潜力及环境安全评价.沈阳农业大学博士学位论文.
23.顾觉奋,陈钢.H-103大孔吸附剂分离纯化抗生素LY-92的研究.离子交换与吸附,1994,10(6):504-509.
24.顾觉奋.抗生素[M].上海:上海科学技术出版社,2001,13-20.
25.顾真荣,吴恩,高新华,等.枯草芽抱杆菌G3菌株的抗菌物质及其特性.植物病理学报,2004,34(2):166-172.
26.郭良栋.内生真菌研究进展.菌物系统,2001,20(1):148-152.
27.韩继刚,宋未.植物内生细菌研究进展及其应用潜力.自然科学进展,2004,14(4):374-379.
28.韩平,西莉,刘鹏飞,等.高效液相色谱法研究啶菌噁唑对番茄灰霉病菌麦角甾醇生物合成的影响.分析化学_研究简报,2006,34(10):1467-1470.
29.何红,蔡学清,陈玉森,等.辣椒内生枯草芽抱杆菌BS-2和BS-1防治香蕉炭疽病研究.福建农林大学学报,2002,31(4):441-443.
30.何红,蔡学清,关雄,等.辣椒内生枯草芽抱杆菌(Bacillus subtilis)BS-2和BS-1防治辣椒炭疽病研究[J].植物病理学报,2003,33(2):170-173.
31.何红,蔡学清,关雄,等.内生菌BS-2菌株的抗菌蛋白及其防病作用.植物病理学报,2003,33(4):373-378.
32.何青芳,陈卫良,马志超.枯草芽抱杆菌A30菌株产生的拮抗肤的分离纯化与理化性质研究.中国水稻科学,2002,16(4):361-365.
33.胡剑,林心怡,张九一,等.拮抗菌BS-98分泌抗菌蛋白的条件及其发酵液特性.微生物学通报,1996,23(6):323-326.
34.胡梁斌.细菌B-FS01抗菌物质的鉴定以及对串珠镰刀菌生长和伏马菌素B1产生的抑制效应.南京:南京农业大学,2007.
35.姬生宝,范晋勇,元英进.藤黄灰链霉菌2H103发酵液中抗真菌活性成分的分离纯化.微生物学通报,2005,32(3):77-81.
36.江木兰.油菜内生枯草芽孢杆菌BY-2的定殖生态、抗菌机制和防治菌核病的研究.华中农业大学博士论文.1997.
37.蒋细良,谢德龄,倪楚芳,等.中生菌素的抗生作用.植物病理学报,1997,27(2):133-138.
38.蒋细良,谢德龄,倪楚芳,等.中生菌素对真菌作用机理的研究.中国生物防治,1997,13(2):69-71.
39.蒋细良,谢德龄.农用抗生素的作用机理.生物防治通报,1994,10(2):76-81.
40.金玲,巴峰,计平生,等.小麦内生有害细菌的发现和作用研究.植物病理学报,1996,26(2)123-126.
41.孔建,王文夕,赵白鸽.枯草芽孢杆菌B-903菌株的研究.中国生物防治,1999,15(4):157-161.
42.孔建,王文夕,赵白鸽,等.枯草芽孢杆菌B-903菌株的研究Ⅲ.影响抗菌物质产生和积累的主要因素.中国生物防治,2000,16(2):65-68.
43.孔建,赵白鸽,王文夕,等.枯草芽抱杆菌抗菌物质对镰刀菌抑制机理的镜下研究.植物病理学报,1998,28(4):337-340.
44.兰琪,姬志勤,顾爱国,等.苦皮藤内生真菌中杀虫杀菌活性物质的初步研究.西北农林科技大学学报,2004,32(10):79-84.
45.黎起泰,叶云峰,王涛,等.内生枯草芽孢杆菌B47菌株入侵番茄的途径及其定殖部位.中国生物防治,24(2):133-137.
46.李星,张汀,杨文香,等.芽抱杆菌对黄瓜霜霉病的防治效果研究.植物保护,2003,29(4):25-27.
47.李州,寥史书,雷文.制药业中溶剂萃取的机制和应用发展方向.中国医药工业杂志,1996,27:89-90.
48.林壁润,谢双大,杨丽梅,等.抗生素2507对蔬菜卵菌病害的防治作用及稳定性研究.植物保护,2000,26(2):11-13.
49.林孔勋.杀菌剂毒理学.北京:中国农业出版社,1992.
50.林茂松.植物线虫病害的生物防治.世界农业,1993,1:43-45.
51.刘焕利,张学君,潘小玫,等.抗菌蛋白产生菌株的筛选.南京农业大学学报,1994,17(增刊):76-78.
52.刘杰凤,韩寒冰,张进凤,等.茄类内生菌的分离及拮抗细菌的筛选.安徽农业科学,2009,37(3):1160-1162.
53.刘明舟,伍学刚,陶黎明,等.农业抗生素94166-23的提取、分离、纯化和结构鉴定.农药学学报,2002,4(3):83-86.
54.刘秋,吴元华,于基成.萃取龟裂链霉菌菌株MY02发酵液中抑菌活性成分SN06的分离纯化.植物保护学报,2004,31(4):353-357.
55.刘西莉.芽孢杆菌HL29的生物学特性和抗菌活性物质研究.北京:中国农业大学博士论文,2004.
56.刘向阳,杨世忠,牟伯中.微生物脂肽的结构.生物技术通报,2005,4:18-21.
57.刘学敏,张春泉,李立军等.菌克毒克(2%宁南霉素水剂)对烟草普通花叶病毒病作用机制的初报.黑龙江烟草,1999,13(5):9-10
58.刘雪.枯草芽孢杆菌B-332菌株发酵条件及其抗菌物质分离纯化的研究.中国农业科学院硕士论文,2007.
59.刘伊强,王雅平.拮抗菌TG26的鉴定及其抗菌蛋白BI的纯化和部分特性.植物学报,1994,36(3):197-203.
60.刘颖,徐庆,陈章良.抗真菌肽LP-1的分离纯化及特性分析.微生物学报,1999,39(5):442-448.
61.刘云霞,张青文,周明样.Bt杀虫基因向水稻内生细菌的转化研究.农业生物技术学报,1987,5(2):188-193.
62.刘云霞,张青文,周明样.电镜免疫胶体金定位水稻内生细菌的研究.农业生物技术学报,1996,4(4):354-358.
63.刘云霞.植物内生细菌的研究与应用.植物保护,1994,20(5):30-32.
64.刘振伟,史秀娟,崔爱平.棉花黄萎病菌提取物对棉花的抗病效果.中国生物防治,2001,17(2):96.
65.刘忠梅,王陵,赵金焕,等.有益内生细菌B946在小麦体内的定殖舰律.中国生物防治,2005,1(2):13-116.
66.龙良鲲,崇刚.内生细凶01-144住番茄根茎内定殖的初步研究.微生物学通报,2003,30(5):53-56.
67.吕玲玲,雷建军,宋明.抗生素的研究及其在农业上的应用.微生物学杂志,2003,23(1):40-45.
68.罗明,芦云,张祥林,等.内生拮抗细菌在哈密瓜植株体内的传导定殖和促生作用研究.西北植物学报,2007,27(4):719-725.
69.马国胜.烟草黑胫病研究进展.烟草科技.2001,(9):44-47.
70.马忠华,周明国,叶钟音.唆枯哇对水稻白叶枯病菌作用机制研究初报.植物病理学报,1997,27(3):237-241.
71.牟志美,路国兵,冀宪领,等.桑树内生拈抗细菌Burkholderia cepacia Lu10-1的分离鉴定及其内生定殖.微生物学报,2008,48(5):623-630.
72.牛书金,别合欣,王少勇,等.白肋烟黑胫病发生规律与防治技术研究.烟草科技,1994,6:38-40.
73.潘文道.芽孢杆菌B-21菌株的生物学特性及其拮抗物质分离纯化研究.广西大学硕士论文.2007.
74.裴炎,李先碧,彭红卫.抗真菌多肽APS-1的分离纯化与特性.微生物学报,1999,39(4):344-349
75.祁高富,杨斌,叶建仁.植物病原真菌毒素研究进展.南京林业大学学报,2000,24(2):66-70.
76.祁之秋,周明国.戊唑醇对小麦纹枯病菌的抑菌作用.农药学学报,2003,5(3):80-84.
77.巨元盛,周淑萍,莫小真,等.稻根联合固氮细菌的研究.微生物学报,1981,21(4):473-476.
78.任欣正.植物病原细菌的分类和鉴定.北京:农业出版社,1992.
79.任争光,张志勇,李丹,等.芽孢杆菌BJ-6产抗菌物质发酵条件初探.中国农学通报,2007,23(5);321-325.
80.隋勤.利迪链霉菌A02拮抗产物的分离纯化和鉴定.东北农业大学硕士学位论文,2007.
81.田长城,许晖曹,柯戴嘉.黑曲霉和芽孢杆菌混合纤维素酶液的活性研究.中国饲料,2008,(3):26-28.
82.田黎,顾真芳,陈杰,等.海洋细菌B-9987菌株产生的抑菌物质及对几种植物病原真菌的作用.植物病理学报,2003,33(1):77-80.
83.田兴山,江学斌,沈会芳,等.高效液相色谱法分离测定农抗2507.华南理工大学学报(自然科学版),2001.4:106-107.
84.田中信男.1977.抗菌素的作用机制(翻泽本).北京:科学出版社.
85.汪澈,何月秋,张永庆.枯草芽孢杆菌B9601-Y2抑菌蛋白活性及产生条件的研究.植物病理学报,2005,35(1):30-36.
86.王刚,李志强.小麦内生细菌的分离及其对小麦纹枯菌的拮抗作用.微生物学通报,2005,(2):20-24.
87.王金生.细菌素在植物细菌病害生防中的应用.生物防治通报,1985,1(2):36-40.
88.王艳红.嘧肽霉素新组分的理化性质、纯化及其结构分析.沈阳农业大学硕士学位论文,2005.
89.吴蔼民,顾本康,傅正擎,等.内生菌73a在不同抗性品种棉花体内的定殖和消长动态研究.植物病理学报,2001,31(4):289-294.
90.吴文君.从天然产物到新农约创制-原理·方法.北京:化学工业出版社,2006.
91.吴学宏,肖建华,张文华,等.2%三唑醇·戊唑醇种衣剂对小麦纹枯病菌麦角甾醇含量的影响.农药学学报,2005,7(4):72-375.
92.夏正俊,顾本康,吴蔼民,等.植株植物内生菌诱导棉花抗黄萎病过程中同工酶活性的变化.江苏农业学报,1997,13(2):99-101.
93.夏正俊,顾本康,吴蔼民.植物内生及根际土壤细菌诱导棉花对大丽轮枝菌抗性的研究[J].中国生物防治,1996,12(1):7-10.
94.辛玉成,秦淑莲,李宝笃,等。枯草芽抱杆菌XM16菌株制剂对苹果霉心病的防治及病原的抑制作用.植物病理学报,2000,30(1):66-70.
95.徐进,何礼远,冯洁,等.0702和GP7-13对植物细菌性青枯病的防治和增产作用.中国生物防治,2004,20(2):138-140.
96.徐静.棉花抗虫工程菌对棉铃虫的离体及活体生物测定.昆虫学报,2000,43(增刊):92-97.
97.徐铮,曹永兵,姜远英.麦角甾醇生物合成途径中的抗真菌药物作用靶酶.国外医药抗生素分册,2001,22(5):193-197.
98.闫孟红,蔡正求,韩继刚,等.植物内生细菌在防治植物病害中的应用研究.生物技术通报,2004,(3):8-13.
99.杨海莲,孙晓璐,宋未,等.水稻内生联合同氮细菌的筛选、鉴定及其分布特性.植物学报,1999,41(9):927-937.
100.杨海莲,孙晓璐,宋未.水稻内生阴沟肠杄菌的定殖研究.自然科学进展.1999,(12):1241-1244.
101.杨海莲,孙晓璐,宋未.植物根际促生细菌和内生细菌的诱导抗病性的研究进展.植物病理学报, 2000,30(2):106-110.
102.杨海莲,孙晓璐,宋未.植物内生细菌的研究.微生物学通报.1998,25(4):224-227.
103.杨海莲,孙晓璐,宋未,等.水稻根面争论产碱菌IAG4的分离、鉴定及其固氮和对病原菌的拮抗作用.植物病理学报,1999,29(4):294-298.
104.杨三维,谢咸升,李红玲,等.几种植物源粗提物对小麦白粉病的防治效果研究.现代农业科技,2008,(11):129-131.
105.叶华智,余桂容,严吉明,等.小麦赤霉病的生物防治研究Ⅲ-拮抗芽孢杆菌B4、B6菌株的防病机制.四川农业大学学报,2003,21(1):18-21.
106.余凤玉,李振华,曾会才.抗真菌农用抗生素研究进展.热带农业科学,2005,25(1):60-65.
107.余桂容,叶华智,张敏,等.小麦赤霉病的生物防治研究.拮抗芽孢杆菌在麦穗上的消长动态及生物学特性.四川农业大学学报,2002,20(4):324-327.
108.袁军,孙福在,田宏先,等.防治马铃薯环腐病有益内生细菌的分离和筛选.微生物学报,2002,42(3):270-274.
109.张炳欣,张平.植物根围外来微生物定殖的检测方法.浙江大学学报(农业与生命科学版),2000,26(3):624-628.
110.张德.2001.井冈霉素A诱导水稻防御纹枯病反应机理研究.中国农业大学中华人民共和国约典.2005版.凡例(八).
111.张宏印,李电东.博莱霉素作用机制研究进展.国外医药抗生素分册,1999,20(6):266-269.
112.张龙翔等.生物化学实验方法与技术.北京:人民教育出版社,1982.
113.张一,杜连祥,马书章,等.大孔吸附树脂纯化万古霉素发酵液的研究.约物生物技术,2007,14(2):127-130.
114.张一宾译.甲氧丙烯酸酯类抗生素开发农用杀菌剂.农药译丛,1995,17(3):25-29.
115.张余良,郭郑.微生物农药应用研究进展.天津农业科学,1996,2(4):32-35.
116.赵世杰,许长成,邹琦,孟庆伟.植物组织中丙二醛测定方法的改进.植物生理学通讯,1994,30(3):207-210.
117.赵秀云,韩素芬.固氮细菌与杨树的联合固氮作用.南京林业大学学报,2001,5(4):17-20.
118.赵秀云,韩素芬.杨树根际固氮菌的分离、筛选和鉴定.南京林业大学学报,2000,24(3):17-20.
119.朱吕雄,谢德玲,倪楚芳.农抗120防治西瓜枯萎病菌的室内药效实验.生物防治通报,1990,6(3):124-127.
120.邹文欣,谭仁样.植物内生菌研究新进展.植物学报,2001,43:881-892.
121.Adhikari,T B.,Joseph,C.M.,Phillips,D.A.and Nelson,M.Evaluation of bacteria isolated from rice for plant growth promotion and biological control of seedling diseases of rice.Can.J.Microbiol. (2001)47,916-924.
122.Alabouvett L,Lemanceau P,SteinbergC.Recent advance in the biological control fusarium wilt.Pestic Sci, 1993,37:365-373.
123.Andrews,J.H.Biological control in phyllosphere.Ann.Rev.Phytopathol,1992,30:603-605.
124.Arai N,Shiomi K,Iwai Y,etal.Argifin,a new chitinase inhibitor,produced by Gliocladium sp.FTD-0668 Ⅱ.Isolation,physico-chemical properties,and structure elucidation.The Journal of Antibiotics,2000,53(6):609-614.
125.AsanoY,Akaishi E,TajimaK,etal.Establishment of monoclonal antibodies that discriminate Bacillus subtilis DB90.Biosci Biotechnol Bioehem,2000,64:652-656.
126.AzevedoJ L,Walter M J,Jose Odair P,et al.Edophytic microorganisms:a review on insect control and recent advances on tropical plants.E JB Electronic J.Biotechnol,2000,3(1):40-65.
127.Bakker P A.The role of siderophores in potato tuber yield increase by Pseudomonas putida in a short rotation of potato.Neth.J.Plant Pathol,1986,92:249-256.
128.Banic S,Lunder M.Additive effect of the combination of griseofulvin and ketoconazole against Microsporum cants in vitro.Mycoses,1989,32(9):487-489.
129.Barraquio,W.L.,Revilla,L.,and Ladha,J.K.Isolation of endophytic diazotrophic bacteria from wetland rice.Plant soil.1997,194:15-24.
130.Benhamou,N.,Klooppcr,J.W.,Quadt-Hallman,A.,Tuzun,S.Induction of defense-related ultrastructoral modifications in pea root tissues inoculated with endophytic bacteria.Plant PhysiOl,1996,112:919-929.
131.Boder U S,Reis V D..In Dolsinelli M,et al.(eds),Mtrogen Fixation Kluwer Academic.Publishers Dordsecht,1991:105-111.
132.Bowyer J,Trumpower B.Rapid reduction of cytochrome c_1 in the presence of antimycin and its implication for the mechanism of electron transfer in the cytochrome bi segment of the mitochondrial respiratory chain. J Biol Chem,1981,256(5):2245-2251.
133.Brooks D S ,Ginzalez C F ,et al.Ph ytopathology.1988,78:626.
134.Buchenauer H.Biological control of soil-borne disease by rhizobacterisJ.Plant Disease and Protection,1995,44:40-50.
135.Carroll G C.The biology of endophytism in plants with particular reference to woody plants [A].In:Fokkema N J,van den Heuvel J eds.Microbiology of the phyllosphere[C].Cambridge U.K.:Cambridge University Press,1986,205-222.
136.Charles W.Bacon,DorothyM.Hinlon Endophytic and Biological Control Potential of Bacillus mojavensis and Related Species.Biological Control,2002,23:274-284.
137.Chelius,M,K.,and Triplett,E.W.Dyadobacter fermentnsgen.nov.,sp nov,a novel Gram-negative bacterium isolated from surface-sterilized Zea mays stems.Int.J.Syst.Bacteriol,2000,50:751-758.
138.Chen C E,Bau Skem,Musson G,etal.Biological control of Fusarium wilt on cotton by use of endophytic bacteria.Biological Control,1995,5:83-91.
139.Chris P,Chanway.Bacterial endophytes.The Encyclopedia of pest management.Marcel Dekker,New York,2002:43-46.
140.Cindy Lodenychx,Jaco Vangronsveld.Endophytic bacteria and their potential applications Critical Reviews in Plant Sciences,2002,21 (6):583-606.
141.Coombs J T,Franco C M M.Visualization of an endophytic Streptomyces species in wheat seed.Appl Environ Microbiol,2003,69(7):4260-4262.
142.Dicarboximide fungicides with their fungicidal activity.Journal of Agricultural Food Chemtry,19 98,46:737-741
143.Dingle,J.Some endophytic fungi reduce the density of pustules of Puccinia reconditaf.sp.tritici in wheat.Mycol.Res,2003,107(3):310-316.
144.Dobereiner J,et al.Endophytic occurrence of diazotrophic bacteria in non-leguminous crops.In :Fendrik I ,et al.(eds)Azospirillum Viand Related Microorganism.Berlin.Springer-V erlag,1995,3-14.
145.Dobereiner J.History and new perspectives of dizazotrophs in association with nonleguminous plants.Symbiosis,1992,13(3):1-13.
146.Dong Z,Canny M,McCully M E,et al.A nitrogen-fixing endophytic of sugarcane stems.Plant physiol,1994,105:1139-1147.
147.Emmert EAB,Handelsman J.Biocontrol of plant diseasese:(Gram-)positive perspective.FEMS Microbiology Letters,1999,171(1):1-9.
148.FENG YJ (冯永君),SONG W(宋未).Characteristics and label loss dynamics of Pantoea ag glomerans,prodominants endophytic bacteria isolate from rice plant.Chinese Journal Biochemistry and Molecular Biology (中国生物化学与分子生物学报),2002,18(1):85-91(in Chinese).
149.Freeman S,etal.Genetic conversion of a fungal plant pathogen to a non pathogenic,en dophytic mutualist.Science,1993,26(3):75-78.
150.Fridlender M,Inbar J,Chet L.Biological contral of soilbrone plant pathogens by a 1,3-glucanase-producing Pseudomonas cepacia.Soil Biol Biochem,1993,25(9):1211-1221.
151.Graner,G,Persson,P,Meijer,J.,etal.A study on mierobial diversity in different cultivars of brassica napus in relation to its wilt pathogen.Vertieillium iongisporum.Ferns Microbial Lett,2003,224(2):269-276.
152.Gyaneshwar P,James EK,MathanN,et al.Endophytic colonization of rice by a diazotrophic strain of Serratia marcescens.J Bacteriol,2001,183(8):2634-2645.
153.Hallmann J,et al.Bacterial endophytes in a gricultural crops.Can J Microbiol,1997,43:895-914.
154.Hecht S M.Bleomycin,new perspectives on the mechanism of action.J Nat Prod,2000,63(1):158-168.
155.Hollis J P.Bacteria in the healthy potato tissue.Phytopathol,1951,41:197-209.
156.Huang J.ultrastructure of bacterial penetration in plants.Ann.Rev.Phytopathol,1986,24:141-157.
157.Huang K.T.1964.e al,J.Antibiotics set.A.17:65-69.
158.Inbar J,Chet 1.Evidence that chitinase produced by Aeromonas caviae is involved in the biolo gical control of soil—borne plant pathogens by this bacteria.Soil.Biolo.Biochem,1991,23:973-978.
159.Jacobs,M.J.,W.M.Bugbee,and D.A.Gabrielson.Enumeration,location and characterization of endophytic bacteria within sugar beet roots.Can.J.Bot.1985,63:1262-1265.
160.Jinkui Zhou,Geoi C,Gerhardt,Andrej Baranski,etal.Capillary lectrophoresis of some tetracycline antibiotics coupled with fast cyclic voltammetric detection.Journal of Chromatograp,1999,839(12):193-201.
161.Juan Calvo,Viviana Calvente,Maria Edith de orellano,Delia Benlia Benuzzi.Biological control of postharvest spoilage caused by Penicillium expansum and Botrytis cinerea in apple by using the bacterium Ra.International Journal of Food Microbiology,2007,113:251-257.
162.Kleinkauf H,Dohren H.Noribosomal bioeynthesis of peptide antibiotis.Europe journal Biochem,1990,192:1-15.
163.Kleopper J W& Beauchamp C J.A review of issues related to measuring colonization of plant roots by bacteria.Can.J.Microbiol,1992,38:1219-1232.
164.Kleopper J W,Schipper B and Bakker P A H M.Proposed elimination of the term endorhizosphere.Phytopathol,1992,82:726-727.
165.KrebsB,OekhardtA,HoedingB,etal.Cyclic peptides from Bacillus amylolique faciensuseful antimycoties,antivirals,fungicides,nematicides etc,1996.
166.Krishnamurthy K.,Gnanamaniekam.Biological control of Rice blast by Pseudomonas fluorescens StrainPf7-14 Evaluation of a Maker Gene and Formulations.Biological Control,1998 (13):158-165.
167.LambT G,Tonkyn D W and Kluepfel D A.Movement of Pseudomonas aurefaciens from the rhizosphere to aerial plant tissue.Can.J.M crobiol,1996,42:1112-1120.
168.Lampel J S.Integrative cloning,Expression,and Stability of the CryLA(c)Genne from Btk in a Recombinant Strain of Clavibacter xyli subsp.cynodontis.Appl.Environ.Microbiol,1994,60(2):501-508.
169.Lebbadi M,Galvez A,Maqueda M,et al.A narrows pectrum peptide antibiotic from Bacillus licheniformis M-4.Journal of Applied Bacteriology,1994,77(1):49-53.
170.Leifert.C,Li,H .etal.Antibiotic Production and Biocontrol Activity by Bacillus subtilise CL27 and Bacillus pumilus CL 45.Journal of Applied bacteriology,1995,78:97-101.
171.Lodewyckx E,et al.Endophytic bacteria and their potential applications.Critical Reviews in Plant Sciences,2002,21:583.
172.Mahaffee W F,Kloepper J W,et al.In “Improving plant production with rhizosphere bacteria” .Ryder M H,Stephenes PM,Bowen G D (eds.).CSIRO,Australia,1994,180.173.Marc-Andre Selosse,Ezekiel Baudoin,Philippe Vandenkoornhuyse.Symbiotic microorganisms,a key for ecological success and protection of plants.C.R.Biologies,2004,327:639-648.
174.Martin,D.F,Priest,F.G.,Todd,C,etal,Distribution of beta-gluconases within the genus Bacillus.Appl.Environ.Micobiol,1980,40(6):1136-1138.
175.Mcinory J A,Koepper J W.In:Molecular Ecology of Rhizosphere Micro-organisms.FOGARA,D.N .Dowling,B.Boesten (Eds).1994,19-28.
176.McInroy,J.A.,and Kloepper,J.W.Survey of indigenous bacterial endophytes from cotton and sweet corn.Plant soil,1995,173,337-342.
177.Moniea LE,ElizabethADJ,WilliamEBJ,et al.Viability and stability of biological control agents on cotton and snap bean seeds pest.Management of Seience,2001,57(8):695-706.
178.Motoo Koitabashi,Seiya Tsushima.Studies on Biocontrol of Air-bore Plant Disease by a Filamentous Fungus Producing Antifugal Volatiles.JARQ,2007,41(4):261-265.
179.Motoo Koitabashi,Yuuji Kajitani,Keita Hirashima.Antifugal substances produced by fungal stain Kyu-W63 from wheat leaf and its taxonomic position.Journal of General Plant Pathlogy,2004,70(2):124-130.
180.Mundt J O and Hinkle N F.Bacteria within ovules and seeds.Appl.Environ.Microbiol,1976,32:694-698.
181.Munif,A.,Halllnann,J.,Sikora,R.A.Induced systemic resistance of seleeted endophytic baeteria against meioidogyne incognito on tomato.Meded Rijksuniv Gent Fak Landboenvkd Toegep Biol Wet.2001,66(2b):663-669.
182.Munimbazi C,Bullerman L.B.Isolation and partial characterization of antifungal Metabolites of Bacillus pumilus.Journal of Applied Microbiology,1998,84(6):959-968.
183.Nakano M M,Zuber P.Molecular biology of antibiotic production in Bacillus IJI.Critieal Reviews in Biotechnology,1990,10(3):223-240.
184.Perini O.Fungal endophytes of treeleaves.In:Andrews J H.Hirano S S.eds.Microbial Ecology of Leaves[C].New York,Springer-Verlag,1991:179-197.
185.Peterson C A ,E manuel M E,etal.Pathway of movement of apoplastic fluorescent dye Tracers through the endodermis at the site of secondary root formation in corn(Zea mays)and broad bean (Viciafaba).Can.J.Bol,1981,59:618-621.
186.Quan CS,Liu Q,Tian WJ,et al.Biodegradation of an endocrine-disrupting chemical,di-2-ethylhexyl phthalate by Bacillus subtilis.No.661 Appl Microbio Biotech,2005,66:701-710.
187.Rerans R,Ruppel S,Jacob H J,et al.Colonization behaviour of two enterobacterial strains on cereals.Biol Fertil Soil,2000,30:550-557.
188.Roy K,Mukhopadhyay T,Reddy G,etal.Mulundocandin,a new lipopeptide antibiotic.I.Taxonomy,fermentation,isolation and characterization.The Journal of Antibiotics,1987,40(3):278-284.
189.Schulz B etal.The endophyte-host interaction:abalanced antagonism.MycolRes,1999,103:1275-1283.
190.Sieber TN et al,Simultaneous stimulation of endophytic Cryptodiaporthe hyxiix and inhibition of Acer macrophyllum callus in dual culture.Mycologia,1990,82:569-575.
191.Silo-Sun L A,Lethbridge B J,Raffel S.J.et al.Biological activities of two fungistatic antibiotics produced by Bacillus cereus UW85.Applied and Enviromental Mico-biology,1994,60(6):2023-2030.
192.Stein T,Borchert S,Conrad B,etal.Two different antibiotic-like peptides originate from the ericingene cluster of Bacillus subtilis A1/3.J.Bacteriology,2002,184:1703-1711.
193.Stephens.C .T,Elmer,W.H.An in vitor assay to evaluate source of resistance in Aspergillus spp.to Fusnrium crown and root rot.Plant Disease,1988,72:334-337.
194.Sturz A V Christie B R,Matheson B G,et al.Biodiversity of endophytic bacteria with colonize red clover nodules,roots,stems and foliage and their influence on host growth.Biol Fertil Soils,1997,25:13-19.
195.Sturz A V,Christie B R,NowakJ.Bacterial endophytes:Potental role in developing sustainable systems of crop production.Critical Reviews in Plant Sciences,2000,19 (1):1-30.
196.Sturz A V.The role of endophytic bacteria during seed piece decay and potato tuberization.Plant and soil,1995,75:57-63.
197.Sturz,A.V.,Christie,B.R.and Nowak,J.Bacterial endophytes:potential role in developing sustainable system of crop production.Crit.Rev.Plant Sci.(2000),19,1-30.
198.Sturz,A.v.,Christie,B.R,Matheson,B.G.,Arsenault,w J.,Buchanan,N.A.(1999)Endophytic.
199.Sugiyama M,Mochmizuki H,Nimi O.Mechanism of protection of protein synthesis against streptomycin inhibition in a producing strain.J Antibiot,1981,34(9):1183-1188.
200.Sulow TV,Shroth M N.Rhizobacteria of sugar beets:Effects of seed application and root colonization on yield.Phytopathol,1982,72:199-206.
201.anaka N.1987.Studies on the mechanism of action of antibiotics. Nippon Saiki-ngaku Zasshi.42(5):707-716.
202.Tang,W.H.Advances in biological control of Plant diseases:proceeding of the international workshop on biological control of plant diseases.China Agricultural University Press,Beijing.1996,38-43.
203.Tjamos ECG,Papavizas R J,Cook,(eds).Biological Control of Plant Disease :Progress and challenges for the Future .Plenum,New York.1992.
204.Tomasino S F,Leister R T,Dimoek M B,et al.Field performance of Clavibacter xyli subsp.Cynodontis expressing the insecticidal protein gene cryLA(c)of Bacillus thuringiensis against European corn borer in field corn.Biological Control,1995,5:442～448.
205.Toyoda,H.and Utsumi,R Method for the prevention of Fusadum diseases and microorganisms used for same U.S.Patent.1988,586.
206.Van Buren A M,Andre C,Ishimaru C A.Biological control of the bacteria ring rot pat hogen by endophytic bacteria isolated from potato.Phytopathology,1993,83:1406.
207.Vargas C,Lopes A and Hemerly.Variability and interactions between endophytic bacteria and fungi isolated from leaf tissues of citrus rootstocks.Can J Miorobiol,2001,47(3):229-236.
208.Velazhahan,R,Samiyappan,R.Relationship between antagonistic activities of Pseudomonas fluorescens islates against Rhizoetonia solani and their production of lyric enzymes.J.Plant Dasease and Protection,1999,106:244-250.
209.Verma S C,Singh A,Chowdhury S P,et al.Endophytic colonization ability of two deep-water rice endophytes,Pantoea sp.and Ochrobactrum sp,using green fluorescent protein reporter.Biotechnol Lett,2004,26:425-429
210.Vollenborich,D,Pauli,G,Ozel,M.,etal.Antimycoplasma properties and application in cell culture of surfactin,a lipopeptide antibiotic from Bacillus subtilis.Appl Environ Microbiol,1997,63(1):44-49.
211.Vollenborich,D.,Ozel,M.,Vater,J.,etal.Mechanism of inactivation of enveloped viruses by the biosurfactant surfactin from Bacillus subtilis.Biologicals,1997,25:289-297.
212.Yaya Rukayadi,Antonius Suwanto,Budi Tjahjona.et al.,Survival and Epiphytic Fitness of a Nonpathogenic Mutant of Xant homonas campestris pv GLycines.Applied and Environmental Microbiology,2000,66(3):1183-1189.
213.Yee Y C,Kisslinger B,Yu V L,Jin D J.A mechanism of rifamycin inhibition and resistance in Pseudomonas aeruginosa.J Antimicrob Chemother,1996,38(1):133-140.
214.Zamfir M,Callewaert R,Cornea P C,et al.Purification and characterization of a bacteriocin produced by Lactobacillus acidophilus IBB801.Journal of Applied Microbiology,1999,87 (6):923-931.
215.Zaumeyer WJ.Seed infection by Bacterium phaseoli.Phytopathology,1929,19:96.
2.陈丽梅,樊妙姬,李玲.甘蔗固氮营养醋杆菌的研究进展.微生物学通报,2000,27(1):63-66.
3.陈文君,吴剑波,全启光.农用抗生素11371各组分的分离纯化和鉴别.中国抗生素杂志,1990,15(1):42-48.
4.陈志谊,刘永锋,陆凡.水稻纹枯病生防菌Bs-916产业化生产关键技术.植物保护学报,2004,31(3):230-233.
5.陈志谊,陆凡,刘春祥,等.水稻纹枯病拮抗细菌B-916培养条件与发酵配方的研究.西北农业学报.1998,12(1):76-8.
6.陈志谊,许志刚,陆凡,等.拮抗细菌B-916对水稻植株的抗性诱导作用.2001,14(2):44-48.
7.崔云龙,刘训理.枯草芽饱杆菌B-921菌株对数种植物病原真菌的抗生作用.生物防治通报,1993,9(1):29-31.
8.崔宗均,苏宝森,蔡妙英,等.北方水稻田固氮细菌资源的研究(简报).中国农业大学学报,1996,1(3):64-80.
9.习亚梅,粟韩,李捷,等.小麦白粉病离体叶段筛选方法初探.浙江化工,2000,31卷增刊:78-80.
10.东秀珠,蔡妙英.常见细菌系统鉴定手册.北京:科学出版社,2001.
11.董合忠,李维江.植物诱导抗病性及其利用.莱阳农学院学报,2001,18(4):268-269.
12.樊连梅.诺尔斯链霉菌西昌变种发酵液宁南霉素分离纯化工艺及其性质研究.沈阳农业大学硕士学位论文,2000.6.
13.方中达,植病研究方法.2004,中国农业出版社.
14.傅正擎,夏正俊,吴蔼民,等.内生菌对棉花黄萎病病菌及毒素的抑制作用和对棉花的促生作用.植物病理学,1999,29(4):374-375.
15.傅正挚等.内生菌对棉花黄萎病病菌及毒素的抑制作用和对棉花的促生作用.植物病理学报,1999,29(4):374-375.
16.高学文,姚仕义,HuongPham,等.枯草芽孢杆菌B2菌株产生的抑菌活性物质分析.中国生物防治,2003,19(3):175-179.
17.高学文,姚仕义,Pham Uong,Vater Joachim,等.基因工程菌枯草芽孢杆菌GEB3产生的脂肽类抗生素及生物活性研究.中国农业大学学报,2003,36(12):1496-1501.
18.高学文,姚仕义,Pham Uong,Vater Joachim,等.枯草芽孢杆菌B2菌株产生的表面活性素变异体的纯化和鉴定.微生物学报,2003,45(5):647-652.
19.高增贵,2007,玉米内生细菌种群多样性与纹枯病生防机理的研究.沈阳农业大学博士学位论文.
20.高增贵,陈捷,刘军华,等.拮抗内生细菌B202006菌株对玉米主要防御酶系的影响.植物病理学报,2007,37(1):102-104.
21.辜运富,张云飞,张小平.一株抗玉米纹枯病内生细菌的分离鉴定及其抗病促生作用.微生物学报,2008,35(8):1240-1245.
22.谷祖敏.2008草茎点霉作为生物除草剂防除鸭跖草的潜力及环境安全评价.沈阳农业大学博士学位论文.
23.顾觉奋,陈钢.H-103大孔吸附剂分离纯化抗生素LY-92的研究.离子交换与吸附,1994,10(6):504-509.
24.顾觉奋.抗生素[M].上海:上海科学技术出版社,2001,13-20.
25.顾真荣,吴恩,高新华,等.枯草芽抱杆菌G3菌株的抗菌物质及其特性.植物病理学报,2004,34(2):166-172.
26.郭良栋.内生真菌研究进展.菌物系统,2001,20(1):148-152.
27.韩继刚,宋未.植物内生细菌研究进展及其应用潜力.自然科学进展,2004,14(4):374-379.
28.韩平,西莉,刘鹏飞,等.高效液相色谱法研究啶菌噁唑对番茄灰霉病菌麦角甾醇生物合成的影响.分析化学_研究简报,2006,34(10):1467-1470.
29.何红,蔡学清,陈玉森,等.辣椒内生枯草芽抱杆菌BS-2和BS-1防治香蕉炭疽病研究.福建农林大学学报,2002,31(4):441-443.
30.何红,蔡学清,关雄,等.辣椒内生枯草芽抱杆菌(Bacillus subtilis)BS-2和BS-1防治辣椒炭疽病研究[J].植物病理学报,2003,33(2):170-173.
31.何红,蔡学清,关雄,等.内生菌BS-2菌株的抗菌蛋白及其防病作用.植物病理学报,2003,33(4):373-378.
32.何青芳,陈卫良,马志超.枯草芽抱杆菌A30菌株产生的拮抗肤的分离纯化与理化性质研究.中国水稻科学,2002,16(4):361-365.
33.胡剑,林心怡,张九一,等.拮抗菌BS-98分泌抗菌蛋白的条件及其发酵液特性.微生物学通报,1996,23(6):323-326.
34.胡梁斌.细菌B-FS01抗菌物质的鉴定以及对串珠镰刀菌生长和伏马菌素B1产生的抑制效应.南京:南京农业大学,2007.
35.姬生宝,范晋勇,元英进.藤黄灰链霉菌2H103发酵液中抗真菌活性成分的分离纯化.微生物学通报,2005,32(3):77-81.
36.江木兰.油菜内生枯草芽孢杆菌BY-2的定殖生态、抗菌机制和防治菌核病的研究.华中农业大学博士论文.1997.
37.蒋细良,谢德龄,倪楚芳,等.中生菌素的抗生作用.植物病理学报,1997,27(2):133-138.
38.蒋细良,谢德龄,倪楚芳,等.中生菌素对真菌作用机理的研究.中国生物防治,1997,13(2):69-71.
39.蒋细良,谢德龄.农用抗生素的作用机理.生物防治通报,1994,10(2):76-81.
40.金玲,巴峰,计平生,等.小麦内生有害细菌的发现和作用研究.植物病理学报,1996,26(2)123-126.
41.孔建,王文夕,赵白鸽.枯草芽孢杆菌B-903菌株的研究.中国生物防治,1999,15(4):157-161.
42.孔建,王文夕,赵白鸽,等.枯草芽孢杆菌B-903菌株的研究Ⅲ.影响抗菌物质产生和积累的主要因素.中国生物防治,2000,16(2):65-68.
43.孔建,赵白鸽,王文夕,等.枯草芽抱杆菌抗菌物质对镰刀菌抑制机理的镜下研究.植物病理学报,1998,28(4):337-340.
44.兰琪,姬志勤,顾爱国,等.苦皮藤内生真菌中杀虫杀菌活性物质的初步研究.西北农林科技大学学报,2004,32(10):79-84.
45.黎起泰,叶云峰,王涛,等.内生枯草芽孢杆菌B47菌株入侵番茄的途径及其定殖部位.中国生物防治,24(2):133-137.
46.李星,张汀,杨文香,等.芽抱杆菌对黄瓜霜霉病的防治效果研究.植物保护,2003,29(4):25-27.
47.李州,寥史书,雷文.制药业中溶剂萃取的机制和应用发展方向.中国医药工业杂志,1996,27:89-90.
48.林壁润,谢双大,杨丽梅,等.抗生素2507对蔬菜卵菌病害的防治作用及稳定性研究.植物保护,2000,26(2):11-13.
49.林孔勋.杀菌剂毒理学.北京:中国农业出版社,1992.
50.林茂松.植物线虫病害的生物防治.世界农业,1993,1:43-45.
51.刘焕利,张学君,潘小玫,等.抗菌蛋白产生菌株的筛选.南京农业大学学报,1994,17(增刊):76-78.
52.刘杰凤,韩寒冰,张进凤,等.茄类内生菌的分离及拮抗细菌的筛选.安徽农业科学,2009,37(3):1160-1162.
53.刘明舟,伍学刚,陶黎明,等.农业抗生素94166-23的提取、分离、纯化和结构鉴定.农药学学报,2002,4(3):83-86.
54.刘秋,吴元华,于基成.萃取龟裂链霉菌菌株MY02发酵液中抑菌活性成分SN06的分离纯化.植物保护学报,2004,31(4):353-357.
55.刘西莉.芽孢杆菌HL29的生物学特性和抗菌活性物质研究.北京:中国农业大学博士论文,2004.
56.刘向阳,杨世忠,牟伯中.微生物脂肽的结构.生物技术通报,2005,4:18-21.
57.刘学敏,张春泉,李立军等.菌克毒克(2%宁南霉素水剂)对烟草普通花叶病毒病作用机制的初报.黑龙江烟草,1999,13(5):9-10
58.刘雪.枯草芽孢杆菌B-332菌株发酵条件及其抗菌物质分离纯化的研究.中国农业科学院硕士论文,2007.
59.刘伊强,王雅平.拮抗菌TG26的鉴定及其抗菌蛋白BI的纯化和部分特性.植物学报,1994,36(3):197-203.
60.刘颖,徐庆,陈章良.抗真菌肽LP-1的分离纯化及特性分析.微生物学报,1999,39(5):442-448.
61.刘云霞,张青文,周明样.Bt杀虫基因向水稻内生细菌的转化研究.农业生物技术学报,1987,5(2):188-193.
62.刘云霞,张青文,周明样.电镜免疫胶体金定位水稻内生细菌的研究.农业生物技术学报,1996,4(4):354-358.
63.刘云霞.植物内生细菌的研究与应用.植物保护,1994,20(5):30-32.
64.刘振伟,史秀娟,崔爱平.棉花黄萎病菌提取物对棉花的抗病效果.中国生物防治,2001,17(2):96.
65.刘忠梅,王陵,赵金焕,等.有益内生细菌B946在小麦体内的定殖舰律.中国生物防治,2005,1(2):13-116.
66.龙良鲲,崇刚.内生细凶01-144住番茄根茎内定殖的初步研究.微生物学通报,2003,30(5):53-56.
67.吕玲玲,雷建军,宋明.抗生素的研究及其在农业上的应用.微生物学杂志,2003,23(1):40-45.
68.罗明,芦云,张祥林,等.内生拮抗细菌在哈密瓜植株体内的传导定殖和促生作用研究.西北植物学报,2007,27(4):719-725.
69.马国胜.烟草黑胫病研究进展.烟草科技.2001,(9):44-47.
70.马忠华,周明国,叶钟音.唆枯哇对水稻白叶枯病菌作用机制研究初报.植物病理学报,1997,27(3):237-241.
71.牟志美,路国兵,冀宪领,等.桑树内生拈抗细菌Burkholderia cepacia Lu10-1的分离鉴定及其内生定殖.微生物学报,2008,48(5):623-630.
72.牛书金,别合欣,王少勇,等.白肋烟黑胫病发生规律与防治技术研究.烟草科技,1994,6:38-40.
73.潘文道.芽孢杆菌B-21菌株的生物学特性及其拮抗物质分离纯化研究.广西大学硕士论文.2007.
74.裴炎,李先碧,彭红卫.抗真菌多肽APS-1的分离纯化与特性.微生物学报,1999,39(4):344-349
75.祁高富,杨斌,叶建仁.植物病原真菌毒素研究进展.南京林业大学学报,2000,24(2):66-70.
76.祁之秋,周明国.戊唑醇对小麦纹枯病菌的抑菌作用.农药学学报,2003,5(3):80-84.
77.巨元盛,周淑萍,莫小真,等.稻根联合固氮细菌的研究.微生物学报,1981,21(4):473-476.
78.任欣正.植物病原细菌的分类和鉴定.北京:农业出版社,1992.
79.任争光,张志勇,李丹,等.芽孢杆菌BJ-6产抗菌物质发酵条件初探.中国农学通报,2007,23(5);321-325.
80.隋勤.利迪链霉菌A02拮抗产物的分离纯化和鉴定.东北农业大学硕士学位论文,2007.
81.田长城,许晖曹,柯戴嘉.黑曲霉和芽孢杆菌混合纤维素酶液的活性研究.中国饲料,2008,(3):26-28.
82.田黎,顾真芳,陈杰,等.海洋细菌B-9987菌株产生的抑菌物质及对几种植物病原真菌的作用.植物病理学报,2003,33(1):77-80.
83.田兴山,江学斌,沈会芳,等.高效液相色谱法分离测定农抗2507.华南理工大学学报(自然科学版),2001.4:106-107.
84.田中信男.1977.抗菌素的作用机制(翻泽本).北京:科学出版社.
85.汪澈,何月秋,张永庆.枯草芽孢杆菌B9601-Y2抑菌蛋白活性及产生条件的研究.植物病理学报,2005,35(1):30-36.
86.王刚,李志强.小麦内生细菌的分离及其对小麦纹枯菌的拮抗作用.微生物学通报,2005,(2):20-24.
87.王金生.细菌素在植物细菌病害生防中的应用.生物防治通报,1985,1(2):36-40.
88.王艳红.嘧肽霉素新组分的理化性质、纯化及其结构分析.沈阳农业大学硕士学位论文,2005.
89.吴蔼民,顾本康,傅正擎,等.内生菌73a在不同抗性品种棉花体内的定殖和消长动态研究.植物病理学报,2001,31(4):289-294.
90.吴文君.从天然产物到新农约创制-原理·方法.北京:化学工业出版社,2006.
91.吴学宏,肖建华,张文华,等.2%三唑醇·戊唑醇种衣剂对小麦纹枯病菌麦角甾醇含量的影响.农药学学报,2005,7(4):72-375.
92.夏正俊,顾本康,吴蔼民,等.植株植物内生菌诱导棉花抗黄萎病过程中同工酶活性的变化.江苏农业学报,1997,13(2):99-101.
93.夏正俊,顾本康,吴蔼民.植物内生及根际土壤细菌诱导棉花对大丽轮枝菌抗性的研究[J].中国生物防治,1996,12(1):7-10.
94.辛玉成,秦淑莲,李宝笃,等。枯草芽抱杆菌XM16菌株制剂对苹果霉心病的防治及病原的抑制作用.植物病理学报,2000,30(1):66-70.
95.徐进,何礼远,冯洁,等.0702和GP7-13对植物细菌性青枯病的防治和增产作用.中国生物防治,2004,20(2):138-140.
96.徐静.棉花抗虫工程菌对棉铃虫的离体及活体生物测定.昆虫学报,2000,43(增刊):92-97.
97.徐铮,曹永兵,姜远英.麦角甾醇生物合成途径中的抗真菌药物作用靶酶.国外医药抗生素分册,2001,22(5):193-197.
98.闫孟红,蔡正求,韩继刚,等.植物内生细菌在防治植物病害中的应用研究.生物技术通报,2004,(3):8-13.
99.杨海莲,孙晓璐,宋未,等.水稻内生联合同氮细菌的筛选、鉴定及其分布特性.植物学报,1999,41(9):927-937.
100.杨海莲,孙晓璐,宋未.水稻内生阴沟肠杄菌的定殖研究.自然科学进展.1999,(12):1241-1244.
101.杨海莲,孙晓璐,宋未.植物根际促生细菌和内生细菌的诱导抗病性的研究进展.植物病理学报, 2000,30(2):106-110.
102.杨海莲,孙晓璐,宋未.植物内生细菌的研究.微生物学通报.1998,25(4):224-227.
103.杨海莲,孙晓璐,宋未,等.水稻根面争论产碱菌IAG4的分离、鉴定及其固氮和对病原菌的拮抗作用.植物病理学报,1999,29(4):294-298.
104.杨三维,谢咸升,李红玲,等.几种植物源粗提物对小麦白粉病的防治效果研究.现代农业科技,2008,(11):129-131.
105.叶华智,余桂容,严吉明,等.小麦赤霉病的生物防治研究Ⅲ-拮抗芽孢杆菌B4、B6菌株的防病机制.四川农业大学学报,2003,21(1):18-21.
106.余凤玉,李振华,曾会才.抗真菌农用抗生素研究进展.热带农业科学,2005,25(1):60-65.
107.余桂容,叶华智,张敏,等.小麦赤霉病的生物防治研究.拮抗芽孢杆菌在麦穗上的消长动态及生物学特性.四川农业大学学报,2002,20(4):324-327.
108.袁军,孙福在,田宏先,等.防治马铃薯环腐病有益内生细菌的分离和筛选.微生物学报,2002,42(3):270-274.
109.张炳欣,张平.植物根围外来微生物定殖的检测方法.浙江大学学报(农业与生命科学版),2000,26(3):624-628.
110.张德.2001.井冈霉素A诱导水稻防御纹枯病反应机理研究.中国农业大学中华人民共和国约典.2005版.凡例(八).
111.张宏印,李电东.博莱霉素作用机制研究进展.国外医药抗生素分册,1999,20(6):266-269.
112.张龙翔等.生物化学实验方法与技术.北京:人民教育出版社,1982.
113.张一,杜连祥,马书章,等.大孔吸附树脂纯化万古霉素发酵液的研究.约物生物技术,2007,14(2):127-130.
114.张一宾译.甲氧丙烯酸酯类抗生素开发农用杀菌剂.农药译丛,1995,17(3):25-29.
115.张余良,郭郑.微生物农药应用研究进展.天津农业科学,1996,2(4):32-35.
116.赵世杰,许长成,邹琦,孟庆伟.植物组织中丙二醛测定方法的改进.植物生理学通讯,1994,30(3):207-210.
117.赵秀云,韩素芬.固氮细菌与杨树的联合固氮作用.南京林业大学学报,2001,5(4):17-20.
118.赵秀云,韩素芬.杨树根际固氮菌的分离、筛选和鉴定.南京林业大学学报,2000,24(3):17-20.
119.朱吕雄,谢德玲,倪楚芳.农抗120防治西瓜枯萎病菌的室内药效实验.生物防治通报,1990,6(3):124-127.
120.邹文欣,谭仁样.植物内生菌研究新进展.植物学报,2001,43:881-892.
121.Adhikari,T B.,Joseph,C.M.,Phillips,D.A.and Nelson,M.Evaluation of bacteria isolated from rice for plant growth promotion and biological control of seedling diseases of rice.Can.J.Microbiol. (2001)47,916-924.
122.Alabouvett L,Lemanceau P,SteinbergC.Recent advance in the biological control fusarium wilt.Pestic Sci, 1993,37:365-373.
123.Andrews,J.H.Biological control in phyllosphere.Ann.Rev.Phytopathol,1992,30:603-605.
124.Arai N,Shiomi K,Iwai Y,etal.Argifin,a new chitinase inhibitor,produced by Gliocladium sp.FTD-0668 Ⅱ.Isolation,physico-chemical properties,and structure elucidation.The Journal of Antibiotics,2000,53(6):609-614.
125.AsanoY,Akaishi E,TajimaK,etal.Establishment of monoclonal antibodies that discriminate Bacillus subtilis DB90.Biosci Biotechnol Bioehem,2000,64:652-656.
126.AzevedoJ L,Walter M J,Jose Odair P,et al.Edophytic microorganisms:a review on insect control and recent advances on tropical plants.E JB Electronic J.Biotechnol,2000,3(1):40-65.
127.Bakker P A.The role of siderophores in potato tuber yield increase by Pseudomonas putida in a short rotation of potato.Neth.J.Plant Pathol,1986,92:249-256.
128.Banic S,Lunder M.Additive effect of the combination of griseofulvin and ketoconazole against Microsporum cants in vitro.Mycoses,1989,32(9):487-489.
129.Barraquio,W.L.,Revilla,L.,and Ladha,J.K.Isolation of endophytic diazotrophic bacteria from wetland rice.Plant soil.1997,194:15-24.
130.Benhamou,N.,Klooppcr,J.W.,Quadt-Hallman,A.,Tuzun,S.Induction of defense-related ultrastructoral modifications in pea root tissues inoculated with endophytic bacteria.Plant PhysiOl,1996,112:919-929.
131.Boder U S,Reis V D..In Dolsinelli M,et al.(eds),Mtrogen Fixation Kluwer Academic.Publishers Dordsecht,1991:105-111.
132.Bowyer J,Trumpower B.Rapid reduction of cytochrome c_1 in the presence of antimycin and its implication for the mechanism of electron transfer in the cytochrome bi segment of the mitochondrial respiratory chain. J Biol Chem,1981,256(5):2245-2251.
133.Brooks D S ,Ginzalez C F ,et al.Ph ytopathology.1988,78:626.
134.Buchenauer H.Biological control of soil-borne disease by rhizobacterisJ.Plant Disease and Protection,1995,44:40-50.
135.Carroll G C.The biology of endophytism in plants with particular reference to woody plants [A].In:Fokkema N J,van den Heuvel J eds.Microbiology of the phyllosphere[C].Cambridge U.K.:Cambridge University Press,1986,205-222.
136.Charles W.Bacon,DorothyM.Hinlon Endophytic and Biological Control Potential of Bacillus mojavensis and Related Species.Biological Control,2002,23:274-284.
137.Chelius,M,K.,and Triplett,E.W.Dyadobacter fermentnsgen.nov.,sp nov,a novel Gram-negative bacterium isolated from surface-sterilized Zea mays stems.Int.J.Syst.Bacteriol,2000,50:751-758.
138.Chen C E,Bau Skem,Musson G,etal.Biological control of Fusarium wilt on cotton by use of endophytic bacteria.Biological Control,1995,5:83-91.
139.Chris P,Chanway.Bacterial endophytes.The Encyclopedia of pest management.Marcel Dekker,New York,2002:43-46.
140.Cindy Lodenychx,Jaco Vangronsveld.Endophytic bacteria and their potential applications Critical Reviews in Plant Sciences,2002,21 (6):583-606.
141.Coombs J T,Franco C M M.Visualization of an endophytic Streptomyces species in wheat seed.Appl Environ Microbiol,2003,69(7):4260-4262.
142.Dicarboximide fungicides with their fungicidal activity.Journal of Agricultural Food Chemtry,19 98,46:737-741
143.Dingle,J.Some endophytic fungi reduce the density of pustules of Puccinia reconditaf.sp.tritici in wheat.Mycol.Res,2003,107(3):310-316.
144.Dobereiner J,et al.Endophytic occurrence of diazotrophic bacteria in non-leguminous crops.In :Fendrik I ,et al.(eds)Azospirillum Viand Related Microorganism.Berlin.Springer-V erlag,1995,3-14.
145.Dobereiner J.History and new perspectives of dizazotrophs in association with nonleguminous plants.Symbiosis,1992,13(3):1-13.
146.Dong Z,Canny M,McCully M E,et al.A nitrogen-fixing endophytic of sugarcane stems.Plant physiol,1994,105:1139-1147.
147.Emmert EAB,Handelsman J.Biocontrol of plant diseasese:(Gram-)positive perspective.FEMS Microbiology Letters,1999,171(1):1-9.
148.FENG YJ (冯永君),SONG W(宋未).Characteristics and label loss dynamics of Pantoea ag glomerans,prodominants endophytic bacteria isolate from rice plant.Chinese Journal Biochemistry and Molecular Biology (中国生物化学与分子生物学报),2002,18(1):85-91(in Chinese).
149.Freeman S,etal.Genetic conversion of a fungal plant pathogen to a non pathogenic,en dophytic mutualist.Science,1993,26(3):75-78.
150.Fridlender M,Inbar J,Chet L.Biological contral of soilbrone plant pathogens by a 1,3-glucanase-producing Pseudomonas cepacia.Soil Biol Biochem,1993,25(9):1211-1221.
151.Graner,G,Persson,P,Meijer,J.,etal.A study on mierobial diversity in different cultivars of brassica napus in relation to its wilt pathogen.Vertieillium iongisporum.Ferns Microbial Lett,2003,224(2):269-276.
152.Gyaneshwar P,James EK,MathanN,et al.Endophytic colonization of rice by a diazotrophic strain of Serratia marcescens.J Bacteriol,2001,183(8):2634-2645.
153.Hallmann J,et al.Bacterial endophytes in a gricultural crops.Can J Microbiol,1997,43:895-914.
154.Hecht S M.Bleomycin,new perspectives on the mechanism of action.J Nat Prod,2000,63(1):158-168.
155.Hollis J P.Bacteria in the healthy potato tissue.Phytopathol,1951,41:197-209.
156.Huang J.ultrastructure of bacterial penetration in plants.Ann.Rev.Phytopathol,1986,24:141-157.
157.Huang K.T.1964.e al,J.Antibiotics set.A.17:65-69.
158.Inbar J,Chet 1.Evidence that chitinase produced by Aeromonas caviae is involved in the biolo gical control of soil—borne plant pathogens by this bacteria.Soil.Biolo.Biochem,1991,23:973-978.
159.Jacobs,M.J.,W.M.Bugbee,and D.A.Gabrielson.Enumeration,location and characterization of endophytic bacteria within sugar beet roots.Can.J.Bot.1985,63:1262-1265.
160.Jinkui Zhou,Geoi C,Gerhardt,Andrej Baranski,etal.Capillary lectrophoresis of some tetracycline antibiotics coupled with fast cyclic voltammetric detection.Journal of Chromatograp,1999,839(12):193-201.
161.Juan Calvo,Viviana Calvente,Maria Edith de orellano,Delia Benlia Benuzzi.Biological control of postharvest spoilage caused by Penicillium expansum and Botrytis cinerea in apple by using the bacterium Ra.International Journal of Food Microbiology,2007,113:251-257.
162.Kleinkauf H,Dohren H.Noribosomal bioeynthesis of peptide antibiotis.Europe journal Biochem,1990,192:1-15.
163.Kleopper J W& Beauchamp C J.A review of issues related to measuring colonization of plant roots by bacteria.Can.J.Microbiol,1992,38:1219-1232.
164.Kleopper J W,Schipper B and Bakker P A H M.Proposed elimination of the term endorhizosphere.Phytopathol,1992,82:726-727.
165.KrebsB,OekhardtA,HoedingB,etal.Cyclic peptides from Bacillus amylolique faciensuseful antimycoties,antivirals,fungicides,nematicides etc,1996.
166.Krishnamurthy K.,Gnanamaniekam.Biological control of Rice blast by Pseudomonas fluorescens StrainPf7-14 Evaluation of a Maker Gene and Formulations.Biological Control,1998 (13):158-165.
167.LambT G,Tonkyn D W and Kluepfel D A.Movement of Pseudomonas aurefaciens from the rhizosphere to aerial plant tissue.Can.J.M crobiol,1996,42:1112-1120.
168.Lampel J S.Integrative cloning,Expression,and Stability of the CryLA(c)Genne from Btk in a Recombinant Strain of Clavibacter xyli subsp.cynodontis.Appl.Environ.Microbiol,1994,60(2):501-508.
169.Lebbadi M,Galvez A,Maqueda M,et al.A narrows pectrum peptide antibiotic from Bacillus licheniformis M-4.Journal of Applied Bacteriology,1994,77(1):49-53.
170.Leifert.C,Li,H .etal.Antibiotic Production and Biocontrol Activity by Bacillus subtilise CL27 and Bacillus pumilus CL 45.Journal of Applied bacteriology,1995,78:97-101.
171.Lodewyckx E,et al.Endophytic bacteria and their potential applications.Critical Reviews in Plant Sciences,2002,21:583.
172.Mahaffee W F,Kloepper J W,et al.In “Improving plant production with rhizosphere bacteria” .Ryder M H,Stephenes PM,Bowen G D (eds.).CSIRO,Australia,1994,180.173.Marc-Andre Selosse,Ezekiel Baudoin,Philippe Vandenkoornhuyse.Symbiotic microorganisms,a key for ecological success and protection of plants.C.R.Biologies,2004,327:639-648.
174.Martin,D.F,Priest,F.G.,Todd,C,etal,Distribution of beta-gluconases within the genus Bacillus.Appl.Environ.Micobiol,1980,40(6):1136-1138.
175.Mcinory J A,Koepper J W.In:Molecular Ecology of Rhizosphere Micro-organisms.FOGARA,D.N .Dowling,B.Boesten (Eds).1994,19-28.
176.McInroy,J.A.,and Kloepper,J.W.Survey of indigenous bacterial endophytes from cotton and sweet corn.Plant soil,1995,173,337-342.
177.Moniea LE,ElizabethADJ,WilliamEBJ,et al.Viability and stability of biological control agents on cotton and snap bean seeds pest.Management of Seience,2001,57(8):695-706.
178.Motoo Koitabashi,Seiya Tsushima.Studies on Biocontrol of Air-bore Plant Disease by a Filamentous Fungus Producing Antifugal Volatiles.JARQ,2007,41(4):261-265.
179.Motoo Koitabashi,Yuuji Kajitani,Keita Hirashima.Antifugal substances produced by fungal stain Kyu-W63 from wheat leaf and its taxonomic position.Journal of General Plant Pathlogy,2004,70(2):124-130.
180.Mundt J O and Hinkle N F.Bacteria within ovules and seeds.Appl.Environ.Microbiol,1976,32:694-698.
181.Munif,A.,Halllnann,J.,Sikora,R.A.Induced systemic resistance of seleeted endophytic baeteria against meioidogyne incognito on tomato.Meded Rijksuniv Gent Fak Landboenvkd Toegep Biol Wet.2001,66(2b):663-669.
182.Munimbazi C,Bullerman L.B.Isolation and partial characterization of antifungal Metabolites of Bacillus pumilus.Journal of Applied Microbiology,1998,84(6):959-968.
183.Nakano M M,Zuber P.Molecular biology of antibiotic production in Bacillus IJI.Critieal Reviews in Biotechnology,1990,10(3):223-240.
184.Perini O.Fungal endophytes of treeleaves.In:Andrews J H.Hirano S S.eds.Microbial Ecology of Leaves[C].New York,Springer-Verlag,1991:179-197.
185.Peterson C A ,E manuel M E,etal.Pathway of movement of apoplastic fluorescent dye Tracers through the endodermis at the site of secondary root formation in corn(Zea mays)and broad bean (Viciafaba).Can.J.Bol,1981,59:618-621.
186.Quan CS,Liu Q,Tian WJ,et al.Biodegradation of an endocrine-disrupting chemical,di-2-ethylhexyl phthalate by Bacillus subtilis.No.661 Appl Microbio Biotech,2005,66:701-710.
187.Rerans R,Ruppel S,Jacob H J,et al.Colonization behaviour of two enterobacterial strains on cereals.Biol Fertil Soil,2000,30:550-557.
188.Roy K,Mukhopadhyay T,Reddy G,etal.Mulundocandin,a new lipopeptide antibiotic.I.Taxonomy,fermentation,isolation and characterization.The Journal of Antibiotics,1987,40(3):278-284.
189.Schulz B etal.The endophyte-host interaction:abalanced antagonism.MycolRes,1999,103:1275-1283.
190.Sieber TN et al,Simultaneous stimulation of endophytic Cryptodiaporthe hyxiix and inhibition of Acer macrophyllum callus in dual culture.Mycologia,1990,82:569-575.
191.Silo-Sun L A,Lethbridge B J,Raffel S.J.et al.Biological activities of two fungistatic antibiotics produced by Bacillus cereus UW85.Applied and Enviromental Mico-biology,1994,60(6):2023-2030.
192.Stein T,Borchert S,Conrad B,etal.Two different antibiotic-like peptides originate from the ericingene cluster of Bacillus subtilis A1/3.J.Bacteriology,2002,184:1703-1711.
193.Stephens.C .T,Elmer,W.H.An in vitor assay to evaluate source of resistance in Aspergillus spp.to Fusnrium crown and root rot.Plant Disease,1988,72:334-337.
194.Sturz A V Christie B R,Matheson B G,et al.Biodiversity of endophytic bacteria with colonize red clover nodules,roots,stems and foliage and their influence on host growth.Biol Fertil Soils,1997,25:13-19.
195.Sturz A V,Christie B R,NowakJ.Bacterial endophytes:Potental role in developing sustainable systems of crop production.Critical Reviews in Plant Sciences,2000,19 (1):1-30.
196.Sturz A V.The role of endophytic bacteria during seed piece decay and potato tuberization.Plant and soil,1995,75:57-63.
197.Sturz,A.V.,Christie,B.R.and Nowak,J.Bacterial endophytes:potential role in developing sustainable system of crop production.Crit.Rev.Plant Sci.(2000),19,1-30.
198.Sturz,A.v.,Christie,B.R,Matheson,B.G.,Arsenault,w J.,Buchanan,N.A.(1999)Endophytic.
199.Sugiyama M,Mochmizuki H,Nimi O.Mechanism of protection of protein synthesis against streptomycin inhibition in a producing strain.J Antibiot,1981,34(9):1183-1188.
200.Sulow TV,Shroth M N.Rhizobacteria of sugar beets:Effects of seed application and root colonization on yield.Phytopathol,1982,72:199-206.
201.anaka N.1987.Studies on the mechanism of action of antibiotics. Nippon Saiki-ngaku Zasshi.42(5):707-716.
202.Tang,W.H.Advances in biological control of Plant diseases:proceeding of the international workshop on biological control of plant diseases.China Agricultural University Press,Beijing.1996,38-43.
203.Tjamos ECG,Papavizas R J,Cook,(eds).Biological Control of Plant Disease :Progress and challenges for the Future .Plenum,New York.1992.
204.Tomasino S F,Leister R T,Dimoek M B,et al.Field performance of Clavibacter xyli subsp.Cynodontis expressing the insecticidal protein gene cryLA(c)of Bacillus thuringiensis against European corn borer in field corn.Biological Control,1995,5:442～448.
205.Toyoda,H.and Utsumi,R Method for the prevention of Fusadum diseases and microorganisms used for same U.S.Patent.1988,586.
206.Van Buren A M,Andre C,Ishimaru C A.Biological control of the bacteria ring rot pat hogen by endophytic bacteria isolated from potato.Phytopathology,1993,83:1406.
207.Vargas C,Lopes A and Hemerly.Variability and interactions between endophytic bacteria and fungi isolated from leaf tissues of citrus rootstocks.Can J Miorobiol,2001,47(3):229-236.
208.Velazhahan,R,Samiyappan,R.Relationship between antagonistic activities of Pseudomonas fluorescens islates against Rhizoetonia solani and their production of lyric enzymes.J.Plant Dasease and Protection,1999,106:244-250.
209.Verma S C,Singh A,Chowdhury S P,et al.Endophytic colonization ability of two deep-water rice endophytes,Pantoea sp.and Ochrobactrum sp,using green fluorescent protein reporter.Biotechnol Lett,2004,26:425-429
210.Vollenborich,D,Pauli,G,Ozel,M.,etal.Antimycoplasma properties and application in cell culture of surfactin,a lipopeptide antibiotic from Bacillus subtilis.Appl Environ Microbiol,1997,63(1):44-49.
211.Vollenborich,D.,Ozel,M.,Vater,J.,etal.Mechanism of inactivation of enveloped viruses by the biosurfactant surfactin from Bacillus subtilis.Biologicals,1997,25:289-297.
212.Yaya Rukayadi,Antonius Suwanto,Budi Tjahjona.et al.,Survival and Epiphytic Fitness of a Nonpathogenic Mutant of Xant homonas campestris pv GLycines.Applied and Environmental Microbiology,2000,66(3):1183-1189.
213.Yee Y C,Kisslinger B,Yu V L,Jin D J.A mechanism of rifamycin inhibition and resistance in Pseudomonas aeruginosa.J Antimicrob Chemother,1996,38(1):133-140.
214.Zamfir M,Callewaert R,Cornea P C,et al.Purification and characterization of a bacteriocin produced by Lactobacillus acidophilus IBB801.Journal of Applied Microbiology,1999,87 (6):923-931.
215.Zaumeyer WJ.Seed infection by Bacterium phaseoli.Phytopathology,1929,19:96.