北五味子多糖理化性质、结构及活性研究
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摘要
五味子(Schisandrachinensis(Turcz)Baill)是国家中药现代化科技产业基地的五大品种之一，特别是北五味子，是五味子中的极品，它含有多糖等活性成分，既可食用也可药用，具有广泛的营养保健作用及药理活性。是优势产业链发展的理想资源，开发前景广阔。本文以北五味子果实为原料，研究北五味子多糖的提取工艺、分离纯化技术、理化性质、结构及活性。以期为北五味子的产业化开发奠定基础。主要结论如下：
1．采用水提醇沉法提取北五味子多糖，通过正交试验确定最佳工艺条件为：料水比1：30；浸提温度100℃；浸提时间3h,在此条件下北五味子多糖得率为10．62％，以提取2次较为合适；若经超声波预处理40min，可使多糖得率从10．62％提高到13．26％；提取液醇沉的优化条件为：5倍体积95％乙醇，醇沉6h。
2．探讨了五种方法对北五味子多糖中蛋白质的脱除效果。结果表明：酶法和三氯乙酸-正丁醇法结合使用效果较好，即酶2％，pH5．0，60℃酶解2h，再用等体积的三氯乙酸-正丁醇溶液处理5次，此时蛋白质含量为0．71mg/g，多糖损失率为17．03％。
3．对比了活性炭和过氧化氢脱色效果，结果表明活性炭对北五味子多糖的脱色效果更好。活性炭脱色的最佳条件为：60℃、pH4．0、添加1．5％的活性炭、搅拌50min，脱色率达91．7％．
4．北五味子多糖过DEAE-52纤维素柱后出现四个洗脱峰，依次为SCP-AⅠ、SCP-BⅠ、SCP-BⅡ和SCP-BⅢ，含量分别占0．42％、4．83％、4．03％和1．25％。将SCP-BⅠ、SCP-BⅡ和SCP-BⅢ分别过Sephadex S-300葡聚糖凝胶柱进一步纯化，冻干后，经醋酸纤维薄膜电泳和Sephadex S-300凝胶过滤等方法鉴定，证明SCP-BⅠ、SCP-BⅡ和SCP-BⅢ均为均一多糖。
5．北五味子多糖理化实验结果表明：三种北五味子多糖比旋光度分别为：+28．0°、+45．0°、+30.0°0。；经X-射线衍射试验结果证明三种北五味子多糖都不是晶体；溶解性实验表明北五味子多糖低温下不易于溶解，溶解性受酸碱度影响较小；粘度特性实验表明北五味子多糖溶液的粘度随溶液浓度增大而增大，随着温度的升高而降低，但温度对其影响具有可逆性；溶液粘度随剪切速率的增加而降低；通过透射电镜和扫描电镜的观察，北五味子多糖分子在极稀的水溶液中呈一定形状的聚集态，其单分子呈线性短棒状，由三股单链螺旋在一起。
6．北五味子多糖的含量分析试验结果表明：SCP-BⅠ、SCP-BⅡ、SCP-BⅢ糖醛酸含量的含量分别为11．03％、10．40％和9．18％，粗多糖糖醛酸含量2．59％-2．96％；SCP-BⅠ、SCP-BⅡ、SCP-BⅢ总糖含量分别为91．3％、90．6％、89．6％、；北五味子多糖元素分析表明，三种多糖是由C、H元素组成，都不含N元素；SCP-BⅠ、SCP-BⅡ、SCP-BⅢ的分子质量为：27Kda、23Kda、15Kda。
7．通过气相色谱(GC)分析结果表明SCP-BⅠ是一种含有GalA酸性杂多糖，其单糖组成主要为阿拉伯糖(Ara)，半乳糖(Gal)，葡萄糖(Glc)和半乳糖醛酸(GalA)，其中摩尔比依次为2．6：3．1：2．1：1．0。
8．SCP-BⅠ经红外光谱分析、核磁共振分析、部分酸水解、高碘酸氧化、Smith降解、甲基化、气相色谱分析、GC-MS分析等方法，确定其分子结构为：
SCP-BⅠ主链主要由Ara(1→5)和Gal(1→6)交替连接组成，其间有痕量的GalA(1→3)；支链由Gal(1→3),Gal(1→4),Glc(1→4)构成；分支点由Glc(1→4，6)，Gal(1→3，6)构成；末端残基为Gal,Glc.SCP-BⅠ存在吡喃糖苷，半乳糖醛酸以内酯形式存在，并且SCP-BⅠ中存在α、β两种构型。
9．北五味子多糖对油脂的抗氧化性能试验结果证明：北五味子多糖对油脂具有一定的抗氧化作用；抗坏血酸与北五味子多糖，对油脂具有较好的抗氧化协同增效作用。
10．通过SCP-BⅠ对·OH和O~(2-)·的清除作用实验，得出SCP-BⅠ对·OH和O~(2-)·都有明显的清除作用，且随着多糖浓度的升高均呈现良好的量效关系。在清除·OH时，当多糖浓度达到35mg／ml时增加幅度减缓，曲线趋于平缓；而在清除O~(2-)·的过程中，当多糖浓度达到43．636mg／ml时增加幅度减缓，SCP-BⅠ对·OH的清除作用优于其对O~(2-)·的清除作用。
11．对D-半乳糖(100mg／Kg.d)致衰老模型小鼠，用400mg／Kg.d、200mg／Kg.d、100mg／Kg.d等剂量的北五味子多糖生理盐水溶液灌胃30d。结果表明，北五味子多糖能显著提高小鼠血清、肝组织、脑组织中SOD、GSH-P_x的活性，减少MDA含量。表明北五味子多糖在抗氧化、防衰老方面具有重要作用
12．北五味子多糖减肥降脂试验结果表明：北五味子多糖能明显降低肥胖小鼠体重、脂肪指数、总胆固醇、甘油三酯、低密度脂蛋白、血糖含量，对肥胖小鼠血清中高密度脂蛋白有明显的升高作用，证明北五味子活性多糖具有明显减肥降脂作用。
13．北五味子多糖降血糖试验表明SCP能降低正常小鼠血糖(P＜0．05)以及四氧嘧啶诱导的糖尿病小鼠血糖(P＜0．01)。SCP还能缓解糖尿病小鼠的症状，恢复小鼠体重，减少饮水量，但对小鼠饮食量影响不显著。
14．建立小鼠游泳训练模型，研究北五味子多糖的抗运动疲劳的作用．结果表明：服用一定剂量北五味子多糖能提高肌糖原和肝糖原含量，减少力竭运动后血乳酸的堆积，维持血糖恒定，使血清酶呈现不同程度的良性变化，延长小鼠力竭时间，并能极显著延长小鼠在缺氧条件下的存活时间，与对照组比较，延长率在14．4％～36．8％之间．本实验充分证明北五味子多糖具有抗运动疲劳的作用。
Schisandra chinensis( Turcz)Baill is One of five varieties of national modernization oftraditional Chinese medicine industry base, Especially schisandrae chinensis is nonsuch inSchisandra chinensis (Turcz)Baill, It contains polysaccharide, etc, Both edible but alsomedicinal,and Widespread nutritional health care function and pharmacological activities. It isthe ideal resources of Industrial development, and Developping prospect is broad.Schisandrachinensis fruit is used as raw materials in this article, Schisandra chinensis polysaccharideextracting technology, purification technology, physical and chemical properties, structure andactivity was studied, It will lay the foundation developping the industrialization ofschisandrae chinensis .Main conclusions are as follows:
1. Polysaccharides are extracted by the methods of water extracting, precipitation withalcohol, and the optimum extraction technology is determined by orthogonal experiments.The optimum parameters to extract are as follows: the ratio of water to material 1:30,temperature 100℃, extracting time 3h and extracting times 2. In this condition, the extractionpercent of polysaccharide is 10.62%. With ultrasound -assisted extraction for over 40 minutesThe rate of extracting Polysaccharide would increased to 13.6% from 10.62% The bestcondition that the extraction and alcohol separated : with 5times volume of 95% ethanol for 6hours.
2. Five methods are used to remove protein from SCP. The results show that the methodof combining enzyme and Trichloroaceticacid-normal-Butanol is better than others. By using2% protease, the polysaccharide solution is enzymic hydrolysisfor 2 hours in 40℃water andpH5.0, added Trichloroaceticacid-normal-Butanol volume as much as polysaccharide solutionfor 5 times, In this condition ,the content of protein is 0.71mg/g, and the rate of lost thePolysaccharide is 17.03%.
3. The decoloration of active carbon and H_2O_2 were studied,the results showed that activecarbon was more effective to decolor SCP than H_2O_2,the optimum conditions were as follows:the decoloration temperature,30℃; pH4.0; the dosage of active carbon, 1%; the decolorationduration, 30 min; The rate of discoloration reach 91.7%
4. SCP without protein is separated into four fractions on DEAE-52 celluloseChromatography. They are SCP-AⅠ、SCP-BⅠ、SCP-BⅡand SCP-BⅢ.The content of them is0.42%、4.83%、4.03% and 1.25%. Then they are purified by SephadexS-300 gel chromatography into SCP-BⅠ、SCP-BⅡand SCP-BⅢ, and After freeze-dried they arehomogeneous tested by cellulose acetate film eletrophoresis and gel filtration on SephadexS-300 column chromatography. The result showed that they was pure component.
5. The result of phisical chemical testing is showed that specific rotation of SCP-BⅠ、SCP-BⅡand SCP-BⅢis +28.0°、+45.0°、+30.0°respectively; The result of X-ray diffractionis showed that they are not crystal, the solubility testing of SCP is showed that they are noteasily dissolved at low temperature and Solubility are less effect by PH. Viscosity propertiesof SCP experiments showed that the viscosity solution of polysaccharides solutionconcentration increases with thickening, Solution viscosity is rreduced with the rise oftemperature, but reversible, Solution viscosity is reduced with the rise of shearing rate ; Byobserving with transmission electron microscope and scanning electron microscopy, SCPpresent Certain shapes state of aggregation in extremely dilute aqueous solution, singlemolecule is linear short clavite, three single chain is spiral together.
6. The content of SCP analysis experiment results showed that The contents of uronicacid are 11.03%、10.40% and 9.18% respectively, and The contents of uronic acid of crudeSCP is 2.59%-2.96%; The content of total sugar of them,is 91.3%、90.6% and 89.6%respectively; The element analysis of SCP showed that three kinds of polysaccharides isconstituted by C and H elements, and they don't contain N elements; The molecular weight ofSCP-BⅠ、SCP-BⅡand SCP-BⅢare estimated to be 27Kda、23Kda、15Kda respectively.
7. Gas chromatography analysis results showed that SCP-BI is an acidheteropolysaccharide consisting of Ara, Gal, Glc and GalA in ratio of 2.6:3.1:2.1:1.
8. SCP-BI was Studied by the infrared spectrum analysis, nuclear magnetic resonance(NMR) analysis, part acid hydrolysis, periodate oxidation, Smithdegradation, methylation,GC and GC-MS analysis method, its molecular structure is:The backbone of SCP-BI wascomposed of (1→5)-linked Ara, (1→6)-linked Gal with 3 branches attached to O-3 of(1→6)-linked Gal and terminated with either Gal (1→3)or Gal(1→4)and Glc(1→4),of Glc and the majority of Gala are distributed in branches. SCP-BI is made ofβ-D-Galactopyranoside; Galaurnic acid and Galaurnic acid exist in lactone and there are twokinds form which isαandβ-configuration.
9. The antioxidant properties test result showed that SCP has strong anti-oxidative effectsfor oil.The SCP and Vc on lard and gingili have synergic antioxidative
10. The experiment which studied on the effect of SCP-BI on scavenging·OH~- andO~(2-)·showed the conclusion that the effect of SCP-BI was obviously on scavenging·OH~- andO~(2-)·and with the more percent of Polysaccharide was added in, there was good relationbetween the number and the effect. When the percent of Polysaccharide was 35mg/ml, itwould not rise sharply as before and the curve was becoming on level .It has the samefunction to O~(2-)·,but the effect is not as good as to·OH~-.It has the same function when theamount must be reached to 43.636mg/ml .So the effect of SCP-BI on scavenging·OH~- isbetter than O~(2-)·.
11. Aging model mice induced by D-galactose(100mg/Kg.d) are respectively igrrigatedwith three doses (400mg/Kg.d, 200mg/Kg.d, 100mg/Kg.d) crude polysaccharide dissolved innormal saline for 30 days. The results show that SCP can increase SOD and GSH-P_X activitiesof serum, liver and brain organization, decrease the contents of their MDA. So the resultsindicate that SCPplays an important role in anti-oxidation and anti-decrepit function.
12. The results of test reducing weight and fat with SCP showed that:the SCP canobviously reduce the adiposity mice's weight,fat index number, TC, TG, LDL and GLU, andimprove their HDL in blood serum too, So the SCP possesses great functions in reducingweight and fat.
13. Pharmaceutical experiments showed SCP administered orally in alloxan-induceddiabetic mice can significantly reduce blood glucose levels (P<0.01)and water intake, andincrease the body weight of diabetic mice compared with alloxan-induced diabetic controlgroup. But no significant influence to of the mice's appetite.
14.Taking male mice as experimental objects, the strength increasing swimming modelswere built up. To Study the Functions of SCP Against exercise Fatigue. The results showedthat the Active SCP can increase the contents of muscle glycogen and liver glycogen, reducethe accumulation of blood lactic acid after exhaustion, maintain the blood sugar constant,raisethe activities of superoxide dismutase and glutathione peroxide in serum, reduce theactivities of creatine kinase and lactatedehydrogenase in serum, and prolong the exhaustingtime of mice and the survival time under hypoxic condition. Compared with controls,prolong the rate at 14.4%～36.8%. It was proved that the Active SCP can alleviate exercisefatigue.
1．采用水提醇沉法提取北五味子多糖，通过正交试验确定最佳工艺条件为：料水比1：30；浸提温度100℃；浸提时间3h,在此条件下北五味子多糖得率为10．62％，以提取2次较为合适；若经超声波预处理40min，可使多糖得率从10．62％提高到13．26％；提取液醇沉的优化条件为：5倍体积95％乙醇，醇沉6h。
2．探讨了五种方法对北五味子多糖中蛋白质的脱除效果。结果表明：酶法和三氯乙酸-正丁醇法结合使用效果较好，即酶2％，pH5．0，60℃酶解2h，再用等体积的三氯乙酸-正丁醇溶液处理5次，此时蛋白质含量为0．71mg/g，多糖损失率为17．03％。
3．对比了活性炭和过氧化氢脱色效果，结果表明活性炭对北五味子多糖的脱色效果更好。活性炭脱色的最佳条件为：60℃、pH4．0、添加1．5％的活性炭、搅拌50min，脱色率达91．7％．
4．北五味子多糖过DEAE-52纤维素柱后出现四个洗脱峰，依次为SCP-AⅠ、SCP-BⅠ、SCP-BⅡ和SCP-BⅢ，含量分别占0．42％、4．83％、4．03％和1．25％。将SCP-BⅠ、SCP-BⅡ和SCP-BⅢ分别过Sephadex S-300葡聚糖凝胶柱进一步纯化，冻干后，经醋酸纤维薄膜电泳和Sephadex S-300凝胶过滤等方法鉴定，证明SCP-BⅠ、SCP-BⅡ和SCP-BⅢ均为均一多糖。
5．北五味子多糖理化实验结果表明：三种北五味子多糖比旋光度分别为：+28．0°、+45．0°、+30.0°0。；经X-射线衍射试验结果证明三种北五味子多糖都不是晶体；溶解性实验表明北五味子多糖低温下不易于溶解，溶解性受酸碱度影响较小；粘度特性实验表明北五味子多糖溶液的粘度随溶液浓度增大而增大，随着温度的升高而降低，但温度对其影响具有可逆性；溶液粘度随剪切速率的增加而降低；通过透射电镜和扫描电镜的观察，北五味子多糖分子在极稀的水溶液中呈一定形状的聚集态，其单分子呈线性短棒状，由三股单链螺旋在一起。
6．北五味子多糖的含量分析试验结果表明：SCP-BⅠ、SCP-BⅡ、SCP-BⅢ糖醛酸含量的含量分别为11．03％、10．40％和9．18％，粗多糖糖醛酸含量2．59％-2．96％；SCP-BⅠ、SCP-BⅡ、SCP-BⅢ总糖含量分别为91．3％、90．6％、89．6％、；北五味子多糖元素分析表明，三种多糖是由C、H元素组成，都不含N元素；SCP-BⅠ、SCP-BⅡ、SCP-BⅢ的分子质量为：27Kda、23Kda、15Kda。
7．通过气相色谱(GC)分析结果表明SCP-BⅠ是一种含有GalA酸性杂多糖，其单糖组成主要为阿拉伯糖(Ara)，半乳糖(Gal)，葡萄糖(Glc)和半乳糖醛酸(GalA)，其中摩尔比依次为2．6：3．1：2．1：1．0。
8．SCP-BⅠ经红外光谱分析、核磁共振分析、部分酸水解、高碘酸氧化、Smith降解、甲基化、气相色谱分析、GC-MS分析等方法，确定其分子结构为：
SCP-BⅠ主链主要由Ara(1→5)和Gal(1→6)交替连接组成，其间有痕量的GalA(1→3)；支链由Gal(1→3),Gal(1→4),Glc(1→4)构成；分支点由Glc(1→4，6)，Gal(1→3，6)构成；末端残基为Gal,Glc.SCP-BⅠ存在吡喃糖苷，半乳糖醛酸以内酯形式存在，并且SCP-BⅠ中存在α、β两种构型。
9．北五味子多糖对油脂的抗氧化性能试验结果证明：北五味子多糖对油脂具有一定的抗氧化作用；抗坏血酸与北五味子多糖，对油脂具有较好的抗氧化协同增效作用。
10．通过SCP-BⅠ对·OH和O~(2-)·的清除作用实验，得出SCP-BⅠ对·OH和O~(2-)·都有明显的清除作用，且随着多糖浓度的升高均呈现良好的量效关系。在清除·OH时，当多糖浓度达到35mg／ml时增加幅度减缓，曲线趋于平缓；而在清除O~(2-)·的过程中，当多糖浓度达到43．636mg／ml时增加幅度减缓，SCP-BⅠ对·OH的清除作用优于其对O~(2-)·的清除作用。
11．对D-半乳糖(100mg／Kg.d)致衰老模型小鼠，用400mg／Kg.d、200mg／Kg.d、100mg／Kg.d等剂量的北五味子多糖生理盐水溶液灌胃30d。结果表明，北五味子多糖能显著提高小鼠血清、肝组织、脑组织中SOD、GSH-P_x的活性，减少MDA含量。表明北五味子多糖在抗氧化、防衰老方面具有重要作用
12．北五味子多糖减肥降脂试验结果表明：北五味子多糖能明显降低肥胖小鼠体重、脂肪指数、总胆固醇、甘油三酯、低密度脂蛋白、血糖含量，对肥胖小鼠血清中高密度脂蛋白有明显的升高作用，证明北五味子活性多糖具有明显减肥降脂作用。
13．北五味子多糖降血糖试验表明SCP能降低正常小鼠血糖(P＜0．05)以及四氧嘧啶诱导的糖尿病小鼠血糖(P＜0．01)。SCP还能缓解糖尿病小鼠的症状，恢复小鼠体重，减少饮水量，但对小鼠饮食量影响不显著。
14．建立小鼠游泳训练模型，研究北五味子多糖的抗运动疲劳的作用．结果表明：服用一定剂量北五味子多糖能提高肌糖原和肝糖原含量，减少力竭运动后血乳酸的堆积，维持血糖恒定，使血清酶呈现不同程度的良性变化，延长小鼠力竭时间，并能极显著延长小鼠在缺氧条件下的存活时间，与对照组比较，延长率在14．4％～36．8％之间．本实验充分证明北五味子多糖具有抗运动疲劳的作用。
Schisandra chinensis( Turcz)Baill is One of five varieties of national modernization oftraditional Chinese medicine industry base, Especially schisandrae chinensis is nonsuch inSchisandra chinensis (Turcz)Baill, It contains polysaccharide, etc, Both edible but alsomedicinal,and Widespread nutritional health care function and pharmacological activities. It isthe ideal resources of Industrial development, and Developping prospect is broad.Schisandrachinensis fruit is used as raw materials in this article, Schisandra chinensis polysaccharideextracting technology, purification technology, physical and chemical properties, structure andactivity was studied, It will lay the foundation developping the industrialization ofschisandrae chinensis .Main conclusions are as follows:
1. Polysaccharides are extracted by the methods of water extracting, precipitation withalcohol, and the optimum extraction technology is determined by orthogonal experiments.The optimum parameters to extract are as follows: the ratio of water to material 1:30,temperature 100℃, extracting time 3h and extracting times 2. In this condition, the extractionpercent of polysaccharide is 10.62%. With ultrasound -assisted extraction for over 40 minutesThe rate of extracting Polysaccharide would increased to 13.6% from 10.62% The bestcondition that the extraction and alcohol separated : with 5times volume of 95% ethanol for 6hours.
2. Five methods are used to remove protein from SCP. The results show that the methodof combining enzyme and Trichloroaceticacid-normal-Butanol is better than others. By using2% protease, the polysaccharide solution is enzymic hydrolysisfor 2 hours in 40℃water andpH5.0, added Trichloroaceticacid-normal-Butanol volume as much as polysaccharide solutionfor 5 times, In this condition ,the content of protein is 0.71mg/g, and the rate of lost thePolysaccharide is 17.03%.
3. The decoloration of active carbon and H_2O_2 were studied,the results showed that activecarbon was more effective to decolor SCP than H_2O_2,the optimum conditions were as follows:the decoloration temperature,30℃; pH4.0; the dosage of active carbon, 1%; the decolorationduration, 30 min; The rate of discoloration reach 91.7%
4. SCP without protein is separated into four fractions on DEAE-52 celluloseChromatography. They are SCP-AⅠ、SCP-BⅠ、SCP-BⅡand SCP-BⅢ.The content of them is0.42%、4.83%、4.03% and 1.25%. Then they are purified by SephadexS-300 gel chromatography into SCP-BⅠ、SCP-BⅡand SCP-BⅢ, and After freeze-dried they arehomogeneous tested by cellulose acetate film eletrophoresis and gel filtration on SephadexS-300 column chromatography. The result showed that they was pure component.
5. The result of phisical chemical testing is showed that specific rotation of SCP-BⅠ、SCP-BⅡand SCP-BⅢis +28.0°、+45.0°、+30.0°respectively; The result of X-ray diffractionis showed that they are not crystal, the solubility testing of SCP is showed that they are noteasily dissolved at low temperature and Solubility are less effect by PH. Viscosity propertiesof SCP experiments showed that the viscosity solution of polysaccharides solutionconcentration increases with thickening, Solution viscosity is rreduced with the rise oftemperature, but reversible, Solution viscosity is reduced with the rise of shearing rate ; Byobserving with transmission electron microscope and scanning electron microscopy, SCPpresent Certain shapes state of aggregation in extremely dilute aqueous solution, singlemolecule is linear short clavite, three single chain is spiral together.
6. The content of SCP analysis experiment results showed that The contents of uronicacid are 11.03%、10.40% and 9.18% respectively, and The contents of uronic acid of crudeSCP is 2.59%-2.96%; The content of total sugar of them,is 91.3%、90.6% and 89.6%respectively; The element analysis of SCP showed that three kinds of polysaccharides isconstituted by C and H elements, and they don't contain N elements; The molecular weight ofSCP-BⅠ、SCP-BⅡand SCP-BⅢare estimated to be 27Kda、23Kda、15Kda respectively.
7. Gas chromatography analysis results showed that SCP-BI is an acidheteropolysaccharide consisting of Ara, Gal, Glc and GalA in ratio of 2.6:3.1:2.1:1.
8. SCP-BI was Studied by the infrared spectrum analysis, nuclear magnetic resonance(NMR) analysis, part acid hydrolysis, periodate oxidation, Smithdegradation, methylation,GC and GC-MS analysis method, its molecular structure is:The backbone of SCP-BI wascomposed of (1→5)-linked Ara, (1→6)-linked Gal with 3 branches attached to O-3 of(1→6)-linked Gal and terminated with either Gal (1→3)or Gal(1→4)and Glc(1→4),of Glc and the majority of Gala are distributed in branches. SCP-BI is made ofβ-D-Galactopyranoside; Galaurnic acid and Galaurnic acid exist in lactone and there are twokinds form which isαandβ-configuration.
9. The antioxidant properties test result showed that SCP has strong anti-oxidative effectsfor oil.The SCP and Vc on lard and gingili have synergic antioxidative
10. The experiment which studied on the effect of SCP-BI on scavenging·OH~- andO~(2-)·showed the conclusion that the effect of SCP-BI was obviously on scavenging·OH~- andO~(2-)·and with the more percent of Polysaccharide was added in, there was good relationbetween the number and the effect. When the percent of Polysaccharide was 35mg/ml, itwould not rise sharply as before and the curve was becoming on level .It has the samefunction to O~(2-)·,but the effect is not as good as to·OH~-.It has the same function when theamount must be reached to 43.636mg/ml .So the effect of SCP-BI on scavenging·OH~- isbetter than O~(2-)·.
11. Aging model mice induced by D-galactose(100mg/Kg.d) are respectively igrrigatedwith three doses (400mg/Kg.d, 200mg/Kg.d, 100mg/Kg.d) crude polysaccharide dissolved innormal saline for 30 days. The results show that SCP can increase SOD and GSH-P_X activitiesof serum, liver and brain organization, decrease the contents of their MDA. So the resultsindicate that SCPplays an important role in anti-oxidation and anti-decrepit function.
12. The results of test reducing weight and fat with SCP showed that:the SCP canobviously reduce the adiposity mice's weight,fat index number, TC, TG, LDL and GLU, andimprove their HDL in blood serum too, So the SCP possesses great functions in reducingweight and fat.
13. Pharmaceutical experiments showed SCP administered orally in alloxan-induceddiabetic mice can significantly reduce blood glucose levels (P<0.01)and water intake, andincrease the body weight of diabetic mice compared with alloxan-induced diabetic controlgroup. But no significant influence to of the mice's appetite.
14.Taking male mice as experimental objects, the strength increasing swimming modelswere built up. To Study the Functions of SCP Against exercise Fatigue. The results showedthat the Active SCP can increase the contents of muscle glycogen and liver glycogen, reducethe accumulation of blood lactic acid after exhaustion, maintain the blood sugar constant,raisethe activities of superoxide dismutase and glutathione peroxide in serum, reduce theactivities of creatine kinase and lactatedehydrogenase in serum, and prolong the exhaustingtime of mice and the survival time under hypoxic condition. Compared with controls,prolong the rate at 14.4%～36.8%. It was proved that the Active SCP can alleviate exercisefatigue.
引文
1．边晓丽，靳菊情，丁东宁，等．2002．长松萝多糖清除氧自由基和抗脂质过氧化反应的研究．中药材，25(3)：188-189．
2．蔡明明，王蕴红，张冰，高顺生．2007．六味地黄汤抗大鼠运动性疲劳实验研究．中国运动医学杂志，26(1)：56-59．
3．蔡曦光，许爱霞，葛斌，等．2005．菟丝子多糖抑制衰老小鼠模型中氧自由基域的作用．第三军医大学学报，27(13)：1326-1328．
4．曹犇．2008．木枣多糖抗小鼠运动疲劳的实验研究．食品科学，29(09)：571-574．
5．曹颖莉，崔荣军，赵璇．2003．螺旋藻多糖对老龄小鼠脑中SOD、MDA的影响．中国初级卫生保健，17(4)：68-69．
6．陈春英，黄雪华，周井岩，等．1998．硫酸化箬叶多糖的结构修饰及其抗艾滋病病毒活性．药学学报，33(4)：264．
7．陈春英，蒋岩．1999．箬叶多糖及其衍生物对小鼠艾滋病作用的研究．中国药理学通报，15(4)：336．
8．陈福君，卢军，张永煜，等．1996．桑的药理研究--桑叶降血糖有效组分对糖尿病动物糖代谢的影响．沈阳药科大学学报，13(1)：24-27．
9．陈海生，王著禄，郑钦岳，等．1995．桑叶多糖-Ⅰ和Ⅱ的结构特性．第二军医大学学报，16(5)：486-487．
10．陈克复．1999．食品流变学及其测量．北京：轻工业出版社，78-89．
11．陈留勇，孟宪军，贾薇，等．2004．黄桃水溶性多糖的抗肿瘤作用及清除自由基、提高免疫活性研究．食品科学，25(2)：167-170．
12．陈学军．2000．药用植物多糖研究进展．海峡药学，11(2)：3-5．
13．陈玉杰，郁建平．2007．雪茶多糖的提取分离及组成研究．济宁医学院学报，(12)：305-306．
14．陈志强，金杨，王昌禄．2008．多糖结构分析方法的研究．饲料工业，(06)：78-83．
15．戴好富，周俊，彭再，等．2001．北五味子的水溶性化学成分．天然产物研究与开发，13(1)：24-26．
16．丁侃等．1999．多糖类药物的毛细管电泳分析方法及其应用．色谱，17：346-350．
17．丁利君，吴倩萍．2003．黄芪多糖的提取及其对自由基的清除作用．食品与机械．(4)：7-9．
18．董李平，吴珍龄．2002．黑豆皮醇提取物清除自由基效果的研究．西南农业大学学报，24(5)：445-449．
19．董群，方积年．1995．寡糖及多糖甲基化方法的发展及现状，天然产物研究与开发，7(2)：60-65．
20．范荣军，田洛，任涛，等．2006．五味子多糖提取及活性炭脱色工艺研究．中草药，37：217-220．
21．方积年，丁侃．2007．天然药物．多糖的主要生物活性及分离纯化方法．中国天然药物， 5(5)：338-345．
22．方一苇．1994．具有药理活性多糖的研究现况，分析化学，22(9)：955-960．
23．傅明辉，林总华．2002．沙棘果水溶性多糖的分离纯化、组分分析及抗氧化活性的研究．食品科学，23(3)：73-75．
24．盖钧镒．2000．试验统计方法．北京：中国农业出版社，74-96．
25．高梅，贾镭，钟越，等．2004．猪苓多糖对小鼠巨噬细胞吞噬功能及IL-1、IL-2活性的影响．中国老年学杂志，24(1)：65-65．
26．高普军，朴云峰，郭晓林，等．1996．北五味子粗多糖保肝作用的机理．白求恩医科大学学报，22(1)：23．
27．葛斌，许爱霞，杨社华．2004．黄芪多糖抗衰老作用机制的研究．中国医院药学杂志，24(10)：610-612．
28．顾佳雯，张陆曦，郭婷婷，等．2007．条斑紫菜多糖抗疲劳生物活性研究．生物技术通报，(4)：120-125．
29．郭敏，陈靠山．2007．牛蒡低聚果糖对小鼠抗疲劳作用的研究．天然产物研究与开发，(19)：642-644．
30．郭欣，高向东，杨晓兵．2004．酸性多糖中的葡萄糖醛酸与中性糖的含量测定．中国生化药物杂志，25(2)：100-101．
31．韩春然．唐娟．2007．黑木耳多糖的酶法提取、纯化及性质研究．食品科学，28(2)：53-55．
32．韩学忠．2005．五味子多糖提取纯化、分级及应用．中医药学报，33(6)：35-36．
33．胡斌杰，陈金锋，王宫南．2007．超声波法与传统热水法提取灵芝多糖的比较研究．食品工业科技，28(2)：190-192．
34．黄纯，马驰．亮菌多糖提取中脱蛋白和脱色方法比较．药学与临床研究，2007，15(1)：45-46．
35．黄芳，蒙义文．2000．活性多糖的研究进展．天然产物研究与开发，11(5)：9098．
36．黄玲，陈华，张捷平．2005．五味子多糖对荷瘤小鼠血液SOD和MDA的影响．福建中医学院学报，15(1)：28-29．
37．黄玲，陈玲，张振林．2004．五味子多糖对荷瘤鼠瘤体抑制作用的病理学观察．中药材，27(3)：202-203．
38．黄玲，张捷平，陈华．2003．五味子多糖对S180荷瘤小鼠抑瘤作用的研究．福建中医学院学报，13(3)：22-23．
39．黄晓青，翟伟菁，张晓玲．2004．沙棘多糖对小鼠实验性高脂血症的影响．营养学报，26(3)：232-234．
40．汲晨锋，邹翔，高世勇，等．2004．3种仙人掌多糖抗肿瘤作用的研究．哈尔滨商业大学学报(自然科学版)，20(2)：127-130．
41．纪耀华，孙莹，张晓滨，等．1999．南五味子中粗多糖提取与总糖含量测定．时珍国医国药，10 (11)：810．
42．江和源，蒋迎．2003．茶叶多糖的微波辅助提取技术研究．食品科技，(10)：17-19．
43．姜峰，赵燕禹，姜梅兰，等．2007．功率超声在中药提取过程中的应用．化工进展，26(7)：944-948．
44．蒋伟哲，孔小龙，段小群，等．2001．龙眼参对机体氧自由基清除作用的研究．中国药房，12(8)：453-454．
45．靳菊情，丁东宁，边晓丽，等．2000．银杏叶多糖的化学及清除羟自由基作用．西安医科大学学报．21(5)：417-419．
46．鞠建华，周亮，等．2001．二维核磁共振波谱在阐明一种三萜多糖皂苷结构中的应用．波谱学杂志，18(4)：229-341．
47．孔庆胜，王艳影，蒋滢．2000．南瓜多糖分离、纯化及其降血脂作用．中国生化药物杂志，21、3)：130-132．
48．来鲁华等．1995．寡糖繁荣构象分析，生物化学与生物物理进展，22(4)：290-294．
49．李尔春．2007．天然植物多糖的结构及活性研究进展．食品工程，(1)：44-46．
50．李贵荣．2002．野菊花多糖的提取及其对活性氧自由基的清除作用．中国公共卫生，18(3)：269-270．
51．李杰，张楚菁．2003．中药多糖的免疫调节及抗肿瘤近年研究概述．天津药学，15(6)：62-64．
52．李洁，何德．2006．生物大分子分离技术．生物技术通报，(3)：49-53．
53．李娟，张耀庭，曾伟等．2000．应用考马斯亮蓝法测定总蛋白含量．中国生物制品学杂志，13(1)：118-120．
54．李巧枝．2007．生物化学实验技术．北京：中国轻工业出版社，33-34．
55．李瑞，赵浩如，陈乃林．2003．白花蛇草多糖除蛋白的方法研究．江苏中医药，24(10)：56-57．
56．李时珍(明)．1975．本草纲目．北京：人民卫生出版社，1238-1241．
57．李小定，荣建华，吴谋成．2003．灰树花多糖PGF-1体外对羟基自由基的抑制作用．食品科学．24(7)：126-130．
58．李雪华，龙盛京．2000．大枣多糖的提取与抗活性氧研究．广西科学，7(1)：54-56．
59．李岩，曲绍春，孙文娟，等．1995．北五味子粗多糖对环磷酰胺所致小鼠免疫功能低下的保护作用．白求恩医科大学学报，21(6)：583．
60．李友元，邓洪波，向大雄，等．2005．黄精多糖的降血脂及抗动脉粥样硬化作用．中国动脉硬化杂志，13(4)：429-431．
61．李知敏，王伯初，周菁，等．2004．植物多糖提取液的几种脱蛋白方法的比较分析．重庆大学学报，27(8)：57-59．
62．廖周坤，姜继祖，王化远，等．1998．超临界CO_2萃取藏药雪灵芝中总皂苷及多糖的研究．中草药，29(9)：601．
63．林雨露．2000．多糖的化学结构及构效关系的研究．武汉教育学院学报，19：34-37．
64．刘成梅，付桂明，等．2002．百合多糖提取的影响因素研究．食品科学，23(2)：87-89．
65．刘成梅，万茵等．2002．百合多糖脱蛋白方法的研究．食品科学，23(1)：89-90．
66．刘翠平，方积年．2001．质谱技术在糖类结构分析中的应用．分析化学，29(6)：716-720．
67．刘放，冯立彬，武生，等．1994．五味予多糖提取分级及性质测定．中医药学报，3：43-44．
68．刘根福，马兰军，毛雁．2007．中药消除运动性疲劳方法学的研究．第四军医大学学报，28(5)：477-479．
69．刘娟，韩晓强，姜博．2006．玉米须多糖治疗糖尿病作用研究．时珍国医国药，17(8)：1441-1442．
70．刘青，李新芳．2001．南沙参多糖对受照射小鼠遗传损伤的拮抗作用．中国药理与临床，17(6)：21-22．．
71．刘颖，金宏，许志勤，等．2006．南瓜多糖对糖尿病大鼠血糖和血脂的影响．中国应用生理学杂志，22(3)：358-361．
72．陆志科，廖威．2003．北五味子化学成分的初步测定．山西大学学报：自然科学版)，6(1)：46-48．
73．吕金顺．2002．植物多糖的凹形结构与生物活性关系．天然产物研究与开发，15(4)，79-82．
74．吕子涛，张书郡，刘楠．1997．五味子多糖的纯化与分级．黑龙江医药，10(2)：90-91．
75．罗基花，胡尚嘉，黄唯莉，等．1997．五味子粗多糖升白细胞作用的初步研究．吉林医学院学报，17(1)：1．
76．罗琼，张声华．2000．枸杞多糖的分离纯化及其抗疲劳作用．卫生研究，29(2)：115-117．
77．苗明三．1997．实验动物和动物实验技术．北京，中国中医药出版社，91-95．
78．苗明三．2002．五味子多糖对衰老模型小鼠的影响．中国医药学报，17(3)：187．
79．苗明三，方晓艳．2003．五味子多糖对正常小鼠免疫功能的影响．中国中医药科技，10(2)：100．
80．莫开菊，谢笔均，汪兴平，刘怀臣．2004．葛仙米多糖的提取、分离与纯化技术研究．食品科学，25(10)：103-108．
81．聂金媛，吴成岩，吴世容，李志良．2004．微波辅助提取茯苓中茯苓多糖的研究．中草药，35(12)：1346-1348．
82．聂凌鸿，宁正祥．2003．广东淮山多糖提取工艺的研究．食品工业科技，24(12)：43-45．
83．庞辉，何惠，李倩茗．2005．螺旋藻抗运动性疲劳作用的实验．中国临床康复，9(28)：200-201．
84．饶颖竹，陈蓉，莫锦坚，等．2004．仙人掌对小鼠实验性高血脂症的降脂作用．中国康复医学杂志，19(7)：523 525．
85．芮海云，吴国荣，陈景耀，等．2003．白芨中性多糖抗氧化作用的实验研究．南京师大学报(自然科学版)，26(4)：94-98．
86．沈其丰，徐广智．1996．13C-核磁共振及其应用．北京：化学工业出版社，258-259．
87．沈映君．2000．《中药药理学》(中医药学高级丛书)．人民卫生出版社．
88．沈自尹，郭为民．2002．枸杞多糖调控老年大鼠T细胞凋亡及相关基因表达的研究．中国免疫学杂志，18(9)：628-630．
89．史德芳．2006．仙人掌多糖提取过程中三种脱蛋白方法的比较研究．现代食品科技，22(4)：93-95．
90．史亚丽，辛晓林，杨立红，蔡德华．2004．香菇多糖对力竭小鼠抗疲劳及保肝作用研究．吉林农业大学学报，26(3)：301-304．
91．睢大员，高普军，吕中智，等．1995．北五味子粗多糖保肝作用的药理研究．吉林中医药，15(1)：37．
92．孙昌波，王森，丁向阳，李贞霞．五味子在食品工业中的应用及其开发前景．食品与机械，2003(6)：9．
93．孙润广，张静．2006．甘草多糖螺旋结构的原子力显微镜研究．化学学报，64(24)：2467-2472．
94．孙炜，王慧铭．2004．昆布多糖对高脂血症大鼠降胆固醇作用及其机理的研究．中国中药杂志，29(10)：1015-1016．
95．谭晓虹，王治宝，李如章．2007．北五味子多糖的提取和含量测定．时珍国医国药，18(6)：1463-9321464．
96．陶占泉，李文辉，何兵．1998．力竭游泳训练后小鼠自由基反应的动态观察．南京师大学报：自然科学版，21(4)：83-87．
97．滕利荣，孟庆繁，刘培元，等．2003．酶法提取百合多糖及其体外抗氧化活性．吉林大学学报(理学版)，41(4)：538-542．
98．田庚元．2002．天然多糖的研究和应用(上)，上海化工，10：29-31．．
99．田云．2002．糖生物学．生物学通报，37(8)：11-12．
100．王克夷．1996．作为信息分子的糖类，化学进展，2(8)：98-108．
101．王莉，顾承志等．2002．微波技术辅助测定北五味子中总黄酮和多糖的含量．食品工业科技，23(5)：70-70．73．
102．王莉，鲁健将，顾承志，等．2001．微波技术在板蓝根多糖提取及含量测定中的应用．中药材，24(3)：180-181．
103．王启荣，吕丹云，周未艾，等．1999．理气扶正类中药消除大鼠运动性疲劳的骨骼肌组织化学研究．中国运动医学杂志，18(4)：300-305．
104．王蓉，吴剑波．2001．多糖生物活性的研究进展，国外医药抗生素分册，22(3)：97-99．
105．王述声．2005．榆耳水溶性多糖GIA的结构分析，高等学校化学学报，26(5)：856-859．
106．王雪松等．2004．降血糖多糖及寡糖的研究进展．药学学报，39(12)：1028-1033．
107．王艳杰，吴勃岩，梁颖．2006．五味子粗多糖拮抗环磷酰胺诱导小鼠微核的实验研究．中医药信息，23(5)：72-73．
108．王扬，王海洪，等．2002．羊栖菜多糖的提取和分离．宁波大学学报，15(1)：53-55．
109．王岳五，张海波，史玉荣，等．2001．甘草多糖GPS对病毒的抑制作用．南开大学学报(自然科学版)，34(2)：126-128．
110．王展，方积年．2000．高场核磁共振波谱在多糖结构研究中的应用．分析化学评述与进展， 28(2)：240-247．
111．王兆梅，李琳，郭祀远等．2002．活性多糖构效关系研究评述．现代化工，22(8)：18-20．
112．王钟敏．2002．X射线衍射分析及其在中草药化学成分结构测定中的应用．河北建筑工程学院学报，(01)：93-97．
113．吴广枫，张拥军，汤坚．2001．超声波用于芦荟多糖提取的研究．食品科技，(6)：23-24．
114．吴桐，高美丰，吴文俊．2003．多糖的抗肿瘤作用研究进展．中国天然药物，1(3)：182-186．
115．夏朝红，戴奇，等．2007．几种多糖的红外光谱研究．武汉理工大学学报，29(1)：47-46．
116．夏建强．2001．运动自由基与抗氧化剂．龙岩师专学院，19(3)：53-55．
117．夏启德，袁翠华．2004．香菇多糖抗疲劳的实验研究．通化师范学院学报，25(4)：77-79．
118．夏泉，刘钢．2007．Seavg法去除粗多糖中蛋白质成分的研究．安徽医药，11(12)：1069-1071．
119．肖炳坤，骆传环，等．2005．气相色谱法测定柴胡多糖的组分．生命科学仪器，3(3)：34-35．
120．肖锡湘，上官新晨．2006．多糖的应用研究．中国食物与营养，(5)：21-22．
121．谢大泽，湛学军，章涛，等．2002．枸杞等5种多糖清除氧自由基的实验研究．江西医学检验，20(4)：195．
122．辛娟，王远亮，郭莉霞，等．2004．多糖的生物活性及其应用．生物医学工程研究，23(10)：179-182．
123．熊冰，高梦祥．2007．超声波辅助浸提南瓜多糖的工艺研究．农产品加工，(1)：64-67．
124．徐叔云，卞如濂，陈修．2002．药理实验方法学．北京：人民卫生出版社，1201-1205．
125．徐淑云．1991．药理实验方法学．北京：人民卫生出版社，1269-1274．
126．徐晓飞，蒋丽，唐健，马忠华．2007．植物多糖的保健功能及开发前景．食品科学，(1)：48-50．
127．薛长勇，腾俊英，邱继红，等．2005．桑叶多糖-肽复合物的降血糖血脂作用．营养学报，27(2)：167-168．
128．薛梅，王鲁石，周静，等．2003．五味子多糖的提取及含量测定．陕西中医，24(3)：267．
129．严仲铠，李万林．1997．中国长白山药用植物彩色图志．北京：人民卫生出版社，187-188．
130．阎俊，李林．2002．海带多糖对小鼠耐缺氧效应及抗疲劳作用．湖北预防医学杂志，13(3)：7-8．
131．杨娟，吴谋成．2001．香菇蛋白多糖抗疲劳作用研究．营养学报，23(4)：350-353．
132．杨云，孟江，冯卫生．2004．大枣酸性多糖ZJ-6的化学研究．食品科学，25(5)：55-58．
133．杨云，田润涛，苗明三等．2004．大枣渣多糖活性炭脱色工艺研究．河南中医学院学报，110(19)：35-36．
134．叶姜瑜，谈锋．2002．紫芝多糖的纯化及组分分析．西南师范大学学报，27(6)：945-949．
135．于晓凤，睢大员，吕忠智，等．1995．五味子粗多糖的初步药理研究．白求恩医科大学学报，21(2)：147．
136．余工．2002．糖生物学研究现状和展望，生物学教学，27(10)：47-49．．
137．曾靖，丛铮．1995．五味子多糖药理作用的初步观察．赣南医学院学报，15(1)：16-19．
138．张安强等．2005．食药用菌多糖的提取分离纯化与结构分析．食用菌学报，12(2)：62-68．
139．张剑波，田庚元．1998．寡糖分离与结构分析进展．生物化学与生物物理学报，25(2)：114-118．
140．张兰杰，张维华，赵珊红．2002．北五味子果实中多糖的提取与纯化研究．鞍山师范学院学报，4(1)：58-60．
141．张莅峡，刘泓，常雅萍．1999．红毛五加多糖抗病毒效应的实验研究．中国中医基础医学杂志，5(3)：25-27．
142．张民，王建华，甘璐，等．2003．枸杞多糖-4组成分析及其生理活性研究．食品与发酵工业，29(2)：22-25．
143．张万秋，朱大勇，林治华．2008．生物多糖的研究进展．重庆工学院学报，22(2)：39-41．
144．张维杰．1999．糖复合物生化研究技术(第二版)．浙江大学出版社，．
145．张伟云．2002．紫菜多糖对免疫细胞剂肿瘤细胞生长的影响．生命科学研究，6(2)：167-170．
146．张艳荣，刘婷婷，王大为．2006．玉米活性多糖减肥降脂作用的研究．食品科学，27(5)：227-230．
147．张英，俞卓裕，吴晓琴．2004．中草药和天然植物有效成分提取新技术-微波协助萃取．中国中药杂志，29(2)：104-108．
148．张蕴琨，丁锡琴，蒋晓玲．1991．举重训练对血清酶和肌红蛋白水平影响．体育科学，11(4)：55-56．
149．赵国华，陈宗道等．2001．活性多糖的研究进展．食品与发酵工业，27(7)：45-48．
150．赵峻，高岚．2004．桑叶多糖的降糖降脂作用．天津中医药，21(6)：505-506．
151．赵伟等．2005．肉苁蓉水溶性多糖SPA的结构分析，高等学校化学学报，26：461-463．
152．赵燕，杨兴斌，等．2006．高效毛细管区带电泳法分析南瓜多糖的单糖组成．中国现代应用药学杂志，23(4)：323-326．
153．郑虎占等．1999．中药现代研究与应用(第六卷)．学苑出版社，5885-5889．
154．郑建仙．1999．功能性食品(第二卷)．北京：中国轻工业出版社，50-65．
155．中华人民共和国药典(一部)．2005．北京：化学工业出版社，48-50．
156．周静．1994．近年来国内植物多糖生物活性进展．中草药，25(1)：40-44．
157．周鹏，谢明勇，傅博强．2001．多糖的结构研究．南昌大学学报(理科版)，25(2)：197-204．
158．周鹏等．2001．茶叶粗多糖的提取及纯化研究．食品科学，22(11)：46-47．
159．周世文，徐传福．1994．多糖的免疫药理作用，中国生化药物杂志，15(2)：143-1475．
160．周宜强主编．1997．糖尿病研治新论．北京：中国医药科技出版社，60-61．
161．朱加进．2002．苦菜中可溶性多糖提取工艺研究．农业工程学报，18(1)：138-142．
162．朱自强．2000．超临界流体萃取技术原理和应用．北京：化学工业出版社．
163．左绍远，罗华君，朱振宇．2001．螺旋藻多糖对糖尿病小鼠抗氧化能力的影响．药物生物技术，8(1)：36-38．
164. Kondakova, N.etal. 2002. New Sructure of 0-Specific Polysaccharides of Proteus. 2. Polysaccharides Containing O-Acetyl Groups.Biokhimiya.Download.
165.Ajabnoor,M.A.1990.Effect of aloes on blood glucose levels in normal and alloxan diabetic mice.Journal of Ethnopharmacology,28,215-220.
166.Allan R Engle,etal.1994.Induced circular dichroism study ofthe aqueous solution complexation ofcello-oligosaccharidesand related polysaccharides with aromatic dyes.Car-bohydrate Research,265:181-195.
167.Andrew N.Round etal.2001.Investigating the nature of branching in pectin by atomic force micros copy and carbohydrate analysis.Carbohydrate Research,331,337-342.
168.B Mulloy,PAS Moura'O,etal.2000.Structure/function studiesof anticoagulant sulphated polysaccharides using NMR.Journal of Biotechnology,77:123-135.
169.Bailey,J.Day,C.1999.Traditional plant medicines as treatments for diabetes.Diabetes Care,12;553-564.
170.BelousovM V,BerexovskayaTP,KomissarenkoNF,etal.1998.Flavonoids of siberian-far-eastern species of rhododendrous of the subspecie,Rhododendrous.Chem Natural Compounds,34(4):510-511.
171.Benavenete-Garcia O,etal.1997.Uses and properties of citrus flavonoids J.Agric.Food Chem,45(12):4505-4516.
172.Benavente-Garcia O,Castillo J,Lorente J.etal.2000.Antioxidant activity of phenolics extracted from olea europaea L.leaves.Food Chem,68:457-462.
173.Bors W,Hellers W,Michel C,etal.1990.Radical chemistry of flavonoid antioxidants.New York:Plenum Press,165-170.
174.Carlo GD etal.1999.Flavonoids:old and new aspects of a class of natural therapeutic drugs.Life Sciences,65(4):337-353.
175.Chen Z Y,Chan P T,Ho K Y,etal.1996.Antioxidant activity of flavonoids is governed by number and location of their aromatic hydroxyl groups.Chem Phys Lipids,79:157-163.
176.Choi Young Hae,Chin Young Won,Kim Jinsoong,etal.1999.Strategies for supercritical fluid extraction of hyoscyamine and scopolamine salts using basified modifiers.Journal of Chromatography,863(1):47-55.
177.Cholbi MR.etal.1991.Inhibitory effect of phenolic compounds on CC14-induced microsomal lipid peroxidation.Experiential,47:195-199.
178.Ciucanu,I.,& Kerek,F.1994.A simple and rapid method for the permethylation of carbohydrates.Carbohydrate Research,131 (2),209～217.
179.Clemens R.2001.Redefining fiber.Food Technology,55(2):100.
180.CookNC and Sammam S.1996.Flavonoids-chemistry,metabolism,cardioprotective effects,and dietary sources(review).Nutritional Biochemisty,7:66-76.
181.Davis S.N.Granner D.K.1996.Insulin,Oral Hypoglycemic Agents,and the Pharmacology of the Endocrine Pancreas.Hardman L.G.,Limbird L.E.,Molinof P.B.Ruddon R.W.Gilman A.G.McGraw-Hill,New York,pp.1487-1518.
182.Dixon,J.S.,& Lipkin,D.1994.Spectrophotometric determination of vicinal glycols.Application to the determination of ribofuranosides.Analytical Chemistry,26,1092～1093.
183.Dong Q,Zheng LY.FangJN.1996.Study on the determination of ogosac-charide and polysaccharide bymodified phenol-sulfuracidmethod.中国中药杂志,31(9)：550．
184.Edge C J,Rademacher TW,Wormald MR.1992.Fast sequencing of oligosaccharides:the eagentarray analysis method.Proc.Natl.Acad.Sci,89(4):6338-6342.
185.Fran,Z G.1998.Polysaccharides inpharmacology.Currentapplicationandfuture con-cepts.PlantaMed,55(6):493-497.
186.G Prabhakar Reddy,Chi-Chou Chang,etal.Determinationby Heteronuclear NMR Spectroscopy of the CompleteStructure of the Cell Wall Polysaccharide of Streptococcussanguis Strain K103.Anal Chem,1993,65:913-921.
187.Gaio T,Ester C,Alberto B,etal.2002.Xanthanandglucomannanmix-tures:synergistic interactions and gelation.Bioma-cromo-lecules,(3):498-504.
188.Gomes,A.,Vedasiromoni,J.R.,Das,M.,Sharma,R.M.,Ganguly,D.K.1995.Antihyperglycemic effect of black tea (Camellia sinensis) in rat.Journal of Ethnopharmacology,45:223～226.
189.Hauke Hilz,Edwin J Bakx,Henk A Schols,etal.2005.Cell wallpolysaccharides in black currants and bilberries-characterization in berries,juice,andpresscake.Carbohydrate Polymers,58:477-488.
190.Hikno H,Ishiyama M,Suzuki Y,etal.1999.Mechanisms of hypoglycemic activity of gandodern B:a glycan of ganoderma lucidum fruit bodies.Planta Medica,,55(2):423-428.
191.Imsa,Kim K,Lee CK.2006.Immunomodulatory activity of polysaccharides isolated from Salicornia herbacea.Int Immunopharmcol,6(9):1451-1458.
192.Ishibashi K Miura NN Adachi Y etal.2001.Relationship between solubility of grifolan,a fungal 1,3-p-D-glucan,and production of tumor necrosis factor by macrophages in vitro.Biosci Biotechnol Biochem,65(9):1993～2000.
193.Ivy J L.1991.Musel glycogen synthesis before and after exercise.Sports Med,11(1):6-19.
194.Jagdzinski PP,Lewandowska M,Januchowski R,etal.2002.The effectofhigh molecular weight dextran sulfate on the production of interleukin-8 in monocyte cell culture.Biomed Phannacother,56(5)254-257.
195.Jimmy Muldoon etal.2002.Structure of two polysaccharides of Campylobacter jejuni 81116.Carbohydrate Research.,337,222.-2229.
196.JonLundqvist,AnitaTeleman,LindaJunel,etal.2002.Isolation and characterization of galac to glu comannan from spruce.Carbohydrate Polymers,48:29-39.
197.Kameswara Rao,B.Giri,R.,Kesavalu,M.M.Apparao,Ch.1997.Herbal medicine:in the management of diabetes mellitus.Manphar Vaidhya Patrika 33-35.
198.Kardosova,Machovae.2006.Antioxidant activity of medicinal plant polysac-charides.Fitoterapia,77(5):367-373.
199.Katherine D,McReynolds,JacquelynGervay-Hague.2000.Exa-mining the secondary structures of unnatural peptides and carbohydrate-based compounds utilizing circular dichrosism.Tetrahedron:A symmetry,11:337-362.
200.Khushbaktova Z A,Yusupova S M,Zamaraeva M V,etal.1996.Interrelationships of the stuctures and antioxidant activities of some flavonoids from the plant of central Asia.Chem Natural Compounds,32(3):338-340.
201.Kiho T,Kochi M,Usui S,etal.2001.Antidiabetic effect of anacidic polysaccharide(TAP) from Tremella aurantia and its degradation product.Biol Pharm Bull,24(12):1400-1403.
202.Kodama N,Harada N.2002.A polysaccharide,extract from Grifola frondosa,induces Th-1 dominant responses in carcinoma-bearing BALB/c mice.Jpn J Pharmacol,904,90(4):357-360.
203.Konno C,Sugiyama K,KanoM.1998.Isolation and hypoglycaemic activity of panaxans A,B,C,D,and E,glycans of Panax ginseng roots.Planta Med,50:434-436.
204.L.J.Miller,J.W.Blunt.2002.Evaluation of the structure of the Polysaccharides from Chondria macrocarpa and Ceramium rubrum as Determined by ~(13)CNMRspectroscopy.Botanica Marina,45,1-8.
205.Lamartiniere,Colal A.2000.Protection against breast cancer with genistein:a component of soy.Am.j.Clin.Nutr,71(6 suppl):1705-1706.
206.Lee B C,Bae JT,Pyoh B,etal.2003.Biological activities of the polysaccharides produced from submer gedculture of the edible BasidiomyceteGrifolafrondosa.EnzymeMicrobTechnol,32(5):574-51.
207.Li J,Han XH,Li ZH,etal.2000.Isolation and determination ofpachyman in Poriacocos(Schw)Wolf[J].Chin J Mod ApplPharm,17(1):49-50.
208.Luetti G B,Steinmuller C,Gifford G E,etal.1999.Macrophage activation by the polysaccharide arabinogalactan isolated from plant cell cultures of Echinaceapurpurea.JNalt Cancer Inst,81(9):669-675.
209.Matsumot O T,Cyong J C,Kiyohar A H,etal.1995.Regulationofimmune com-plexesbind-ingofmacrophages by pectic polysaccharide from Bupleurumfal-catumL.Pharmacological evidence for the requirement of intracellular calci-um/calmodulin on Fcreceptor up-regulation by bupleuran 2b.Journal of Pharmacy andPharmacology,47(2):152-156.
210.Matsumot OT,Kiyohar AH,Yamad AH,etal.1996.The pectic polysaccharide from Bupleurum falcatumL.enhances clearance of immune complexes in mice.PhytotherapyResearch,10(7):585-588.
211.Michael Miller,MD.2003.Raising an isolated low HDL-C levehWhy,how,and when?.Cleveland Clinic Journal of Medicine,6 (70):560.
212.Mock,K.K.etal,1991.The Analysis of Underivatized Oligosacchrides by Matrix-assisted Laser Desorption Mass Spectrometry,Biochem Biophys Res Comm,177(2):644-651.
213.Mota A.etal.1990.Struture-activity relationships of polymethxyflavones and other flavonoids as inhabitorys of non-enzymic lipid peroxidation.Biochem Pharmacol,40:793-797.
214.N.E.Awad.1994.Lipid content and antimicrobial activity of phenolic constituents of cultivated Portulaca Oleracea L.Bull.Fac.Pharm.Cairo Univ.,32 (1):137-142.
215.Naomi Sakai,Kyouko Inada.1996.Michi Odamoto.Portuloside A,a monoterpene glucoside from portulaca oleracea.Phytochemistry,42 (6):1625-1628.
216.Nicola,Volpi.2003.Milligram-scale preparation and purificationof oligosaccharides of defined length possessing thestructure of chondroitin from defructosylated capsularpolysaccharide K4.Glycobiology,13(9):635-640.
217.Ourdia Bouzid,David Navarro,Marjolaine Roche,etal.2005.Fungal enzymes as a powerful tool to release simple phenoliccompounds from olive oil by-products.Process Biochemistry,40:1855-1862.
218.Oussama etc.2002.Fungal cell-wall galactomannans isolated from Geotrichum spp.And their teleomorphs,Dipodascus and Galactomyces.Carbohydrate Research.337:2347-2351.
219.P.Jackson.1994.The analysis of fluorophore-labelled glycans by high-resolution lyacrylamide gel eleterophoresis.Anal Biochem,216(2):243-252.
220.P Valachovi,A Pechova,T J Mason.2001.Towards theindustrial production of medicinaltincture by ultrasound assistedextr action.Ultrason.Sonochem,(8):111-117.
221.Peng Zhi-ying,Zhang Ning,Zhong Yan-lin.2002.Molecular Structure of Microbial Exo polysaccharides PS-9415.Journal of South China University of Technology,30 (3):78-82.
222.PigeoletE.1990.GSH-Px,SOD and CAT inactivation by peroxidesOxgen derived free radicals.MechAgeingDev,50:283-297.
223.Pinto GLD,Martinez M,Corredor ALD,etal.1996.Structural studies of the polysaccharide isolated from Spondias purpurea gum exudates.Carbohydrate Res,290:97-103.
224.Qin Chuanguang,Huang Kaixun,Xu Huibi.2002.Isolation and characterization of a novel polysaccharide from the mucus of the loach,Misgurnus anguillicaudatus.Carbohydrate polymers,49:367-371.
225.Roman-Ramos,R.,Flores-Saenz,J.L.Alarcon-Aguilar,F.J.1995.Antihyperglycemic effect of some edible plants.Journal of Ethnopharmacology,48,25-32.
226.SaitoH,Yoshioka Y,Aketagawa NUJ,etal.1997.Relationship between conformation and biological response for(1→3)-β-D-glucans in theactivation ofcoagulation Factor G from limulus amebocyte lysate andhost-mediated antitumor activity.Demonstration of single-helixconformation as a stimulant.Carbohydrate Research.217:181-190.55.
227.Saul Roseman.2001.Reflections on Glycobiology,JBC,276(45):41527-41542.
228.SlavomirBystricky,ShousunCSzu,etal.1995.circular dich-roism of the o-specific polysaccharide of vibrio choleraeol and some related derivatives.Carbohyydrate research,270:115-122.
229.Sofya N.Senchenkova,etal.2000.Structure of the O-specific polysaccharides of the lippo polysaccharides of Xanthomonas campestris PV.vignicola GSPB 2795 and GSPB 2796.Carbohydrate Research,329,329:831-838.
230.Stimpelm,Prokscha,Wagnerh,etal.1994.Macrophage activation and in-duction ofmacrophage cytotoxicity by purified polysaccharide fractions fromtheplantEchinaceapurpurea.Infect Immun,46(3):845-849.
231.Susumu Honda,Shigeo Suzuki,etal.2003.Analysis of carbohyd-rates as 1-pheny 1-3-methy 1-5-pyrazolone derivatives by capillary/microchip electrophoresis andcapillaryelectro-chromatography.JournalofPharmaceuticalandBiomedicalAnalysis,30:1689-1714.
232.Szkudelski T.2001.The mechanism of alloxan and streptozotocin action in p cells of the rat pancreas.Physiol Res,50:536-546.
233.Tang W,Heman I,Bertram B.2003.Recent development of antitumor agents from Chinese herbal medicines.Partll.High molecular lompounds.Panta Med,69:193-201..
234.Taylor,R.L.,Conrad,H.E.1992.Stoichiometric depolymerization of polyuronides and glycosamino glycuronans to monosaccharides following reduction of their carbodiimide-activated carboxyl groups.Biochemistry,11,1383—1388.
235.Unursaikhan Surenjav,Lina Zhang,Xiaojuan Xu,etal.2006.Effects of molecular structure on antitumor activities of (1—>3)-p-D-glucan from different Lentinus Edodes.Car-bohydrate Polymers.63(63):97-104.
236.V.N Reinhold,B.B.Reinhold,S.Chan.1996.Carbohydrate sequence analysis by electrospray ionization mass spectrometry.Methods enzymol,271:377-402.
237.W.S.Hancock,A.Appffel,etal.1996.Reversed-phase peptide mapping of glycoprotcins using liquid chromatography/eletrospray ionization-mass spectrometry.Methods Enzymol,271:403-427.
238.Wang,H.X.,Ng,T.B.1999.Natural products with hypoglycemic,hypotensive,hypocholesterolemic,antiatherosclerotic and antithrombotic activities.Life Sciences,65:2663～ 2677.
239.Wang,Z.J.,Luo,D.H.,& Liang,Z.Y.2004.Structure of polysaccharides from the fruiting body of Hericium erinaceus Pers.Carbohydrate Polymers,57,241-247.
240.Wang J H,Wang S J,Wei W C,etal.2004.Use of plant Polysaccharidesas topi-cally applied adjuvants for enhancing immune responses viaTransdermal Gene Vaccination.MolTher,9(S1):207.
241.Wermurh C.G 1992.Meditional Che mistry for the 21st century (IUPAC Che mistry for the 21~(st) Century),London:Blackwell Scientific Publications,38-84..
242.Whitworth W,Charles C P,Joseph E,etal.1990.Macrophages and cancer,Cancer and Metasis,319-322.
243.Xiang D B,Li X Y.1993.Antitumor actity and immuno-potentiating actions of Achyanthes bidentata polysaccharides.Acta Pharmacol.Sin,14(6):556-561.
244.Xu Jing,Loo George.2001.Different effects of genistein on molecular markers related to apoptosis in two phenotypically dissimilar breast cancer line.Cell Biochem,82(1):78-88.
245.XuZH,ZhouSW.2001.Progress of plant polysaccharides and its hypoglycemiceffects.West China J Pharm Sci,16(5):365-366.
246.Yadav S,Vats V,Dhunnoo Y,Grover JK.2002.Hypoglycemic and antihyperglycemic activity of Murraya koenigii leaves in diabetic rats.J Ethnopharmacol,82:111 ～ 116.
247.Yoon SJ,Pereira MS,Pavaom SG,etal.2002.The medicinal plant Poranavolubiliscontains polysaccharides with anticoagulant activity mediated by hep-arin cofactor Ⅱ.ThrombRes, 106(1): 59-62.
248. ZHrom(?)dkov(?), A Ebringerov(?), P Valachovi. 2002. Ultrasound-assisted extraction of water-soluble polysaccharides from theroots of valerian. Ultrason Sonochem, (9):37-44.
249. ZHrom(?)dkov(?), AEbringerov(?), PValachovi(?). 1999. Comparison of classicalandultrasound-assisted extraction of polysaccharides from Salvia officinalis L. Ultrason Sonochem, (5): 163-168.
2．蔡明明，王蕴红，张冰，高顺生．2007．六味地黄汤抗大鼠运动性疲劳实验研究．中国运动医学杂志，26(1)：56-59．
3．蔡曦光，许爱霞，葛斌，等．2005．菟丝子多糖抑制衰老小鼠模型中氧自由基域的作用．第三军医大学学报，27(13)：1326-1328．
4．曹犇．2008．木枣多糖抗小鼠运动疲劳的实验研究．食品科学，29(09)：571-574．
5．曹颖莉，崔荣军，赵璇．2003．螺旋藻多糖对老龄小鼠脑中SOD、MDA的影响．中国初级卫生保健，17(4)：68-69．
6．陈春英，黄雪华，周井岩，等．1998．硫酸化箬叶多糖的结构修饰及其抗艾滋病病毒活性．药学学报，33(4)：264．
7．陈春英，蒋岩．1999．箬叶多糖及其衍生物对小鼠艾滋病作用的研究．中国药理学通报，15(4)：336．
8．陈福君，卢军，张永煜，等．1996．桑的药理研究--桑叶降血糖有效组分对糖尿病动物糖代谢的影响．沈阳药科大学学报，13(1)：24-27．
9．陈海生，王著禄，郑钦岳，等．1995．桑叶多糖-Ⅰ和Ⅱ的结构特性．第二军医大学学报，16(5)：486-487．
10．陈克复．1999．食品流变学及其测量．北京：轻工业出版社，78-89．
11．陈留勇，孟宪军，贾薇，等．2004．黄桃水溶性多糖的抗肿瘤作用及清除自由基、提高免疫活性研究．食品科学，25(2)：167-170．
12．陈学军．2000．药用植物多糖研究进展．海峡药学，11(2)：3-5．
13．陈玉杰，郁建平．2007．雪茶多糖的提取分离及组成研究．济宁医学院学报，(12)：305-306．
14．陈志强，金杨，王昌禄．2008．多糖结构分析方法的研究．饲料工业，(06)：78-83．
15．戴好富，周俊，彭再，等．2001．北五味子的水溶性化学成分．天然产物研究与开发，13(1)：24-26．
16．丁侃等．1999．多糖类药物的毛细管电泳分析方法及其应用．色谱，17：346-350．
17．丁利君，吴倩萍．2003．黄芪多糖的提取及其对自由基的清除作用．食品与机械．(4)：7-9．
18．董李平，吴珍龄．2002．黑豆皮醇提取物清除自由基效果的研究．西南农业大学学报，24(5)：445-449．
19．董群，方积年．1995．寡糖及多糖甲基化方法的发展及现状，天然产物研究与开发，7(2)：60-65．
20．范荣军，田洛，任涛，等．2006．五味子多糖提取及活性炭脱色工艺研究．中草药，37：217-220．
21．方积年，丁侃．2007．天然药物．多糖的主要生物活性及分离纯化方法．中国天然药物， 5(5)：338-345．
22．方一苇．1994．具有药理活性多糖的研究现况，分析化学，22(9)：955-960．
23．傅明辉，林总华．2002．沙棘果水溶性多糖的分离纯化、组分分析及抗氧化活性的研究．食品科学，23(3)：73-75．
24．盖钧镒．2000．试验统计方法．北京：中国农业出版社，74-96．
25．高梅，贾镭，钟越，等．2004．猪苓多糖对小鼠巨噬细胞吞噬功能及IL-1、IL-2活性的影响．中国老年学杂志，24(1)：65-65．
26．高普军，朴云峰，郭晓林，等．1996．北五味子粗多糖保肝作用的机理．白求恩医科大学学报，22(1)：23．
27．葛斌，许爱霞，杨社华．2004．黄芪多糖抗衰老作用机制的研究．中国医院药学杂志，24(10)：610-612．
28．顾佳雯，张陆曦，郭婷婷，等．2007．条斑紫菜多糖抗疲劳生物活性研究．生物技术通报，(4)：120-125．
29．郭敏，陈靠山．2007．牛蒡低聚果糖对小鼠抗疲劳作用的研究．天然产物研究与开发，(19)：642-644．
30．郭欣，高向东，杨晓兵．2004．酸性多糖中的葡萄糖醛酸与中性糖的含量测定．中国生化药物杂志，25(2)：100-101．
31．韩春然．唐娟．2007．黑木耳多糖的酶法提取、纯化及性质研究．食品科学，28(2)：53-55．
32．韩学忠．2005．五味子多糖提取纯化、分级及应用．中医药学报，33(6)：35-36．
33．胡斌杰，陈金锋，王宫南．2007．超声波法与传统热水法提取灵芝多糖的比较研究．食品工业科技，28(2)：190-192．
34．黄纯，马驰．亮菌多糖提取中脱蛋白和脱色方法比较．药学与临床研究，2007，15(1)：45-46．
35．黄芳，蒙义文．2000．活性多糖的研究进展．天然产物研究与开发，11(5)：9098．
36．黄玲，陈华，张捷平．2005．五味子多糖对荷瘤小鼠血液SOD和MDA的影响．福建中医学院学报，15(1)：28-29．
37．黄玲，陈玲，张振林．2004．五味子多糖对荷瘤鼠瘤体抑制作用的病理学观察．中药材，27(3)：202-203．
38．黄玲，张捷平，陈华．2003．五味子多糖对S180荷瘤小鼠抑瘤作用的研究．福建中医学院学报，13(3)：22-23．
39．黄晓青，翟伟菁，张晓玲．2004．沙棘多糖对小鼠实验性高脂血症的影响．营养学报，26(3)：232-234．
40．汲晨锋，邹翔，高世勇，等．2004．3种仙人掌多糖抗肿瘤作用的研究．哈尔滨商业大学学报(自然科学版)，20(2)：127-130．
41．纪耀华，孙莹，张晓滨，等．1999．南五味子中粗多糖提取与总糖含量测定．时珍国医国药，10 (11)：810．
42．江和源，蒋迎．2003．茶叶多糖的微波辅助提取技术研究．食品科技，(10)：17-19．
43．姜峰，赵燕禹，姜梅兰，等．2007．功率超声在中药提取过程中的应用．化工进展，26(7)：944-948．
44．蒋伟哲，孔小龙，段小群，等．2001．龙眼参对机体氧自由基清除作用的研究．中国药房，12(8)：453-454．
45．靳菊情，丁东宁，边晓丽，等．2000．银杏叶多糖的化学及清除羟自由基作用．西安医科大学学报．21(5)：417-419．
46．鞠建华，周亮，等．2001．二维核磁共振波谱在阐明一种三萜多糖皂苷结构中的应用．波谱学杂志，18(4)：229-341．
47．孔庆胜，王艳影，蒋滢．2000．南瓜多糖分离、纯化及其降血脂作用．中国生化药物杂志，21、3)：130-132．
48．来鲁华等．1995．寡糖繁荣构象分析，生物化学与生物物理进展，22(4)：290-294．
49．李尔春．2007．天然植物多糖的结构及活性研究进展．食品工程，(1)：44-46．
50．李贵荣．2002．野菊花多糖的提取及其对活性氧自由基的清除作用．中国公共卫生，18(3)：269-270．
51．李杰，张楚菁．2003．中药多糖的免疫调节及抗肿瘤近年研究概述．天津药学，15(6)：62-64．
52．李洁，何德．2006．生物大分子分离技术．生物技术通报，(3)：49-53．
53．李娟，张耀庭，曾伟等．2000．应用考马斯亮蓝法测定总蛋白含量．中国生物制品学杂志，13(1)：118-120．
54．李巧枝．2007．生物化学实验技术．北京：中国轻工业出版社，33-34．
55．李瑞，赵浩如，陈乃林．2003．白花蛇草多糖除蛋白的方法研究．江苏中医药，24(10)：56-57．
56．李时珍(明)．1975．本草纲目．北京：人民卫生出版社，1238-1241．
57．李小定，荣建华，吴谋成．2003．灰树花多糖PGF-1体外对羟基自由基的抑制作用．食品科学．24(7)：126-130．
58．李雪华，龙盛京．2000．大枣多糖的提取与抗活性氧研究．广西科学，7(1)：54-56．
59．李岩，曲绍春，孙文娟，等．1995．北五味子粗多糖对环磷酰胺所致小鼠免疫功能低下的保护作用．白求恩医科大学学报，21(6)：583．
60．李友元，邓洪波，向大雄，等．2005．黄精多糖的降血脂及抗动脉粥样硬化作用．中国动脉硬化杂志，13(4)：429-431．
61．李知敏，王伯初，周菁，等．2004．植物多糖提取液的几种脱蛋白方法的比较分析．重庆大学学报，27(8)：57-59．
62．廖周坤，姜继祖，王化远，等．1998．超临界CO_2萃取藏药雪灵芝中总皂苷及多糖的研究．中草药，29(9)：601．
63．林雨露．2000．多糖的化学结构及构效关系的研究．武汉教育学院学报，19：34-37．
64．刘成梅，付桂明，等．2002．百合多糖提取的影响因素研究．食品科学，23(2)：87-89．
65．刘成梅，万茵等．2002．百合多糖脱蛋白方法的研究．食品科学，23(1)：89-90．
66．刘翠平，方积年．2001．质谱技术在糖类结构分析中的应用．分析化学，29(6)：716-720．
67．刘放，冯立彬，武生，等．1994．五味予多糖提取分级及性质测定．中医药学报，3：43-44．
68．刘根福，马兰军，毛雁．2007．中药消除运动性疲劳方法学的研究．第四军医大学学报，28(5)：477-479．
69．刘娟，韩晓强，姜博．2006．玉米须多糖治疗糖尿病作用研究．时珍国医国药，17(8)：1441-1442．
70．刘青，李新芳．2001．南沙参多糖对受照射小鼠遗传损伤的拮抗作用．中国药理与临床，17(6)：21-22．．
71．刘颖，金宏，许志勤，等．2006．南瓜多糖对糖尿病大鼠血糖和血脂的影响．中国应用生理学杂志，22(3)：358-361．
72．陆志科，廖威．2003．北五味子化学成分的初步测定．山西大学学报：自然科学版)，6(1)：46-48．
73．吕金顺．2002．植物多糖的凹形结构与生物活性关系．天然产物研究与开发，15(4)，79-82．
74．吕子涛，张书郡，刘楠．1997．五味子多糖的纯化与分级．黑龙江医药，10(2)：90-91．
75．罗基花，胡尚嘉，黄唯莉，等．1997．五味子粗多糖升白细胞作用的初步研究．吉林医学院学报，17(1)：1．
76．罗琼，张声华．2000．枸杞多糖的分离纯化及其抗疲劳作用．卫生研究，29(2)：115-117．
77．苗明三．1997．实验动物和动物实验技术．北京，中国中医药出版社，91-95．
78．苗明三．2002．五味子多糖对衰老模型小鼠的影响．中国医药学报，17(3)：187．
79．苗明三，方晓艳．2003．五味子多糖对正常小鼠免疫功能的影响．中国中医药科技，10(2)：100．
80．莫开菊，谢笔均，汪兴平，刘怀臣．2004．葛仙米多糖的提取、分离与纯化技术研究．食品科学，25(10)：103-108．
81．聂金媛，吴成岩，吴世容，李志良．2004．微波辅助提取茯苓中茯苓多糖的研究．中草药，35(12)：1346-1348．
82．聂凌鸿，宁正祥．2003．广东淮山多糖提取工艺的研究．食品工业科技，24(12)：43-45．
83．庞辉，何惠，李倩茗．2005．螺旋藻抗运动性疲劳作用的实验．中国临床康复，9(28)：200-201．
84．饶颖竹，陈蓉，莫锦坚，等．2004．仙人掌对小鼠实验性高血脂症的降脂作用．中国康复医学杂志，19(7)：523 525．
85．芮海云，吴国荣，陈景耀，等．2003．白芨中性多糖抗氧化作用的实验研究．南京师大学报(自然科学版)，26(4)：94-98．
86．沈其丰，徐广智．1996．13C-核磁共振及其应用．北京：化学工业出版社，258-259．
87．沈映君．2000．《中药药理学》(中医药学高级丛书)．人民卫生出版社．
88．沈自尹，郭为民．2002．枸杞多糖调控老年大鼠T细胞凋亡及相关基因表达的研究．中国免疫学杂志，18(9)：628-630．
89．史德芳．2006．仙人掌多糖提取过程中三种脱蛋白方法的比较研究．现代食品科技，22(4)：93-95．
90．史亚丽，辛晓林，杨立红，蔡德华．2004．香菇多糖对力竭小鼠抗疲劳及保肝作用研究．吉林农业大学学报，26(3)：301-304．
91．睢大员，高普军，吕中智，等．1995．北五味子粗多糖保肝作用的药理研究．吉林中医药，15(1)：37．
92．孙昌波，王森，丁向阳，李贞霞．五味子在食品工业中的应用及其开发前景．食品与机械，2003(6)：9．
93．孙润广，张静．2006．甘草多糖螺旋结构的原子力显微镜研究．化学学报，64(24)：2467-2472．
94．孙炜，王慧铭．2004．昆布多糖对高脂血症大鼠降胆固醇作用及其机理的研究．中国中药杂志，29(10)：1015-1016．
95．谭晓虹，王治宝，李如章．2007．北五味子多糖的提取和含量测定．时珍国医国药，18(6)：1463-9321464．
96．陶占泉，李文辉，何兵．1998．力竭游泳训练后小鼠自由基反应的动态观察．南京师大学报：自然科学版，21(4)：83-87．
97．滕利荣，孟庆繁，刘培元，等．2003．酶法提取百合多糖及其体外抗氧化活性．吉林大学学报(理学版)，41(4)：538-542．
98．田庚元．2002．天然多糖的研究和应用(上)，上海化工，10：29-31．．
99．田云．2002．糖生物学．生物学通报，37(8)：11-12．
100．王克夷．1996．作为信息分子的糖类，化学进展，2(8)：98-108．
101．王莉，顾承志等．2002．微波技术辅助测定北五味子中总黄酮和多糖的含量．食品工业科技，23(5)：70-70．73．
102．王莉，鲁健将，顾承志，等．2001．微波技术在板蓝根多糖提取及含量测定中的应用．中药材，24(3)：180-181．
103．王启荣，吕丹云，周未艾，等．1999．理气扶正类中药消除大鼠运动性疲劳的骨骼肌组织化学研究．中国运动医学杂志，18(4)：300-305．
104．王蓉，吴剑波．2001．多糖生物活性的研究进展，国外医药抗生素分册，22(3)：97-99．
105．王述声．2005．榆耳水溶性多糖GIA的结构分析，高等学校化学学报，26(5)：856-859．
106．王雪松等．2004．降血糖多糖及寡糖的研究进展．药学学报，39(12)：1028-1033．
107．王艳杰，吴勃岩，梁颖．2006．五味子粗多糖拮抗环磷酰胺诱导小鼠微核的实验研究．中医药信息，23(5)：72-73．
108．王扬，王海洪，等．2002．羊栖菜多糖的提取和分离．宁波大学学报，15(1)：53-55．
109．王岳五，张海波，史玉荣，等．2001．甘草多糖GPS对病毒的抑制作用．南开大学学报(自然科学版)，34(2)：126-128．
110．王展，方积年．2000．高场核磁共振波谱在多糖结构研究中的应用．分析化学评述与进展， 28(2)：240-247．
111．王兆梅，李琳，郭祀远等．2002．活性多糖构效关系研究评述．现代化工，22(8)：18-20．
112．王钟敏．2002．X射线衍射分析及其在中草药化学成分结构测定中的应用．河北建筑工程学院学报，(01)：93-97．
113．吴广枫，张拥军，汤坚．2001．超声波用于芦荟多糖提取的研究．食品科技，(6)：23-24．
114．吴桐，高美丰，吴文俊．2003．多糖的抗肿瘤作用研究进展．中国天然药物，1(3)：182-186．
115．夏朝红，戴奇，等．2007．几种多糖的红外光谱研究．武汉理工大学学报，29(1)：47-46．
116．夏建强．2001．运动自由基与抗氧化剂．龙岩师专学院，19(3)：53-55．
117．夏启德，袁翠华．2004．香菇多糖抗疲劳的实验研究．通化师范学院学报，25(4)：77-79．
118．夏泉，刘钢．2007．Seavg法去除粗多糖中蛋白质成分的研究．安徽医药，11(12)：1069-1071．
119．肖炳坤，骆传环，等．2005．气相色谱法测定柴胡多糖的组分．生命科学仪器，3(3)：34-35．
120．肖锡湘，上官新晨．2006．多糖的应用研究．中国食物与营养，(5)：21-22．
121．谢大泽，湛学军，章涛，等．2002．枸杞等5种多糖清除氧自由基的实验研究．江西医学检验，20(4)：195．
122．辛娟，王远亮，郭莉霞，等．2004．多糖的生物活性及其应用．生物医学工程研究，23(10)：179-182．
123．熊冰，高梦祥．2007．超声波辅助浸提南瓜多糖的工艺研究．农产品加工，(1)：64-67．
124．徐叔云，卞如濂，陈修．2002．药理实验方法学．北京：人民卫生出版社，1201-1205．
125．徐淑云．1991．药理实验方法学．北京：人民卫生出版社，1269-1274．
126．徐晓飞，蒋丽，唐健，马忠华．2007．植物多糖的保健功能及开发前景．食品科学，(1)：48-50．
127．薛长勇，腾俊英，邱继红，等．2005．桑叶多糖-肽复合物的降血糖血脂作用．营养学报，27(2)：167-168．
128．薛梅，王鲁石，周静，等．2003．五味子多糖的提取及含量测定．陕西中医，24(3)：267．
129．严仲铠，李万林．1997．中国长白山药用植物彩色图志．北京：人民卫生出版社，187-188．
130．阎俊，李林．2002．海带多糖对小鼠耐缺氧效应及抗疲劳作用．湖北预防医学杂志，13(3)：7-8．
131．杨娟，吴谋成．2001．香菇蛋白多糖抗疲劳作用研究．营养学报，23(4)：350-353．
132．杨云，孟江，冯卫生．2004．大枣酸性多糖ZJ-6的化学研究．食品科学，25(5)：55-58．
133．杨云，田润涛，苗明三等．2004．大枣渣多糖活性炭脱色工艺研究．河南中医学院学报，110(19)：35-36．
134．叶姜瑜，谈锋．2002．紫芝多糖的纯化及组分分析．西南师范大学学报，27(6)：945-949．
135．于晓凤，睢大员，吕忠智，等．1995．五味子粗多糖的初步药理研究．白求恩医科大学学报，21(2)：147．
136．余工．2002．糖生物学研究现状和展望，生物学教学，27(10)：47-49．．
137．曾靖，丛铮．1995．五味子多糖药理作用的初步观察．赣南医学院学报，15(1)：16-19．
138．张安强等．2005．食药用菌多糖的提取分离纯化与结构分析．食用菌学报，12(2)：62-68．
139．张剑波，田庚元．1998．寡糖分离与结构分析进展．生物化学与生物物理学报，25(2)：114-118．
140．张兰杰，张维华，赵珊红．2002．北五味子果实中多糖的提取与纯化研究．鞍山师范学院学报，4(1)：58-60．
141．张莅峡，刘泓，常雅萍．1999．红毛五加多糖抗病毒效应的实验研究．中国中医基础医学杂志，5(3)：25-27．
142．张民，王建华，甘璐，等．2003．枸杞多糖-4组成分析及其生理活性研究．食品与发酵工业，29(2)：22-25．
143．张万秋，朱大勇，林治华．2008．生物多糖的研究进展．重庆工学院学报，22(2)：39-41．
144．张维杰．1999．糖复合物生化研究技术(第二版)．浙江大学出版社，．
145．张伟云．2002．紫菜多糖对免疫细胞剂肿瘤细胞生长的影响．生命科学研究，6(2)：167-170．
146．张艳荣，刘婷婷，王大为．2006．玉米活性多糖减肥降脂作用的研究．食品科学，27(5)：227-230．
147．张英，俞卓裕，吴晓琴．2004．中草药和天然植物有效成分提取新技术-微波协助萃取．中国中药杂志，29(2)：104-108．
148．张蕴琨，丁锡琴，蒋晓玲．1991．举重训练对血清酶和肌红蛋白水平影响．体育科学，11(4)：55-56．
149．赵国华，陈宗道等．2001．活性多糖的研究进展．食品与发酵工业，27(7)：45-48．
150．赵峻，高岚．2004．桑叶多糖的降糖降脂作用．天津中医药，21(6)：505-506．
151．赵伟等．2005．肉苁蓉水溶性多糖SPA的结构分析，高等学校化学学报，26：461-463．
152．赵燕，杨兴斌，等．2006．高效毛细管区带电泳法分析南瓜多糖的单糖组成．中国现代应用药学杂志，23(4)：323-326．
153．郑虎占等．1999．中药现代研究与应用(第六卷)．学苑出版社，5885-5889．
154．郑建仙．1999．功能性食品(第二卷)．北京：中国轻工业出版社，50-65．
155．中华人民共和国药典(一部)．2005．北京：化学工业出版社，48-50．
156．周静．1994．近年来国内植物多糖生物活性进展．中草药，25(1)：40-44．
157．周鹏，谢明勇，傅博强．2001．多糖的结构研究．南昌大学学报(理科版)，25(2)：197-204．
158．周鹏等．2001．茶叶粗多糖的提取及纯化研究．食品科学，22(11)：46-47．
159．周世文，徐传福．1994．多糖的免疫药理作用，中国生化药物杂志，15(2)：143-1475．
160．周宜强主编．1997．糖尿病研治新论．北京：中国医药科技出版社，60-61．
161．朱加进．2002．苦菜中可溶性多糖提取工艺研究．农业工程学报，18(1)：138-142．
162．朱自强．2000．超临界流体萃取技术原理和应用．北京：化学工业出版社．
163．左绍远，罗华君，朱振宇．2001．螺旋藻多糖对糖尿病小鼠抗氧化能力的影响．药物生物技术，8(1)：36-38．
164. Kondakova, N.etal. 2002. New Sructure of 0-Specific Polysaccharides of Proteus. 2. Polysaccharides Containing O-Acetyl Groups.Biokhimiya.Download.
165.Ajabnoor,M.A.1990.Effect of aloes on blood glucose levels in normal and alloxan diabetic mice.Journal of Ethnopharmacology,28,215-220.
166.Allan R Engle,etal.1994.Induced circular dichroism study ofthe aqueous solution complexation ofcello-oligosaccharidesand related polysaccharides with aromatic dyes.Car-bohydrate Research,265:181-195.
167.Andrew N.Round etal.2001.Investigating the nature of branching in pectin by atomic force micros copy and carbohydrate analysis.Carbohydrate Research,331,337-342.
168.B Mulloy,PAS Moura'O,etal.2000.Structure/function studiesof anticoagulant sulphated polysaccharides using NMR.Journal of Biotechnology,77:123-135.
169.Bailey,J.Day,C.1999.Traditional plant medicines as treatments for diabetes.Diabetes Care,12;553-564.
170.BelousovM V,BerexovskayaTP,KomissarenkoNF,etal.1998.Flavonoids of siberian-far-eastern species of rhododendrous of the subspecie,Rhododendrous.Chem Natural Compounds,34(4):510-511.
171.Benavenete-Garcia O,etal.1997.Uses and properties of citrus flavonoids J.Agric.Food Chem,45(12):4505-4516.
172.Benavente-Garcia O,Castillo J,Lorente J.etal.2000.Antioxidant activity of phenolics extracted from olea europaea L.leaves.Food Chem,68:457-462.
173.Bors W,Hellers W,Michel C,etal.1990.Radical chemistry of flavonoid antioxidants.New York:Plenum Press,165-170.
174.Carlo GD etal.1999.Flavonoids:old and new aspects of a class of natural therapeutic drugs.Life Sciences,65(4):337-353.
175.Chen Z Y,Chan P T,Ho K Y,etal.1996.Antioxidant activity of flavonoids is governed by number and location of their aromatic hydroxyl groups.Chem Phys Lipids,79:157-163.
176.Choi Young Hae,Chin Young Won,Kim Jinsoong,etal.1999.Strategies for supercritical fluid extraction of hyoscyamine and scopolamine salts using basified modifiers.Journal of Chromatography,863(1):47-55.
177.Cholbi MR.etal.1991.Inhibitory effect of phenolic compounds on CC14-induced microsomal lipid peroxidation.Experiential,47:195-199.
178.Ciucanu,I.,& Kerek,F.1994.A simple and rapid method for the permethylation of carbohydrates.Carbohydrate Research,131 (2),209～217.
179.Clemens R.2001.Redefining fiber.Food Technology,55(2):100.
180.CookNC and Sammam S.1996.Flavonoids-chemistry,metabolism,cardioprotective effects,and dietary sources(review).Nutritional Biochemisty,7:66-76.
181.Davis S.N.Granner D.K.1996.Insulin,Oral Hypoglycemic Agents,and the Pharmacology of the Endocrine Pancreas.Hardman L.G.,Limbird L.E.,Molinof P.B.Ruddon R.W.Gilman A.G.McGraw-Hill,New York,pp.1487-1518.
182.Dixon,J.S.,& Lipkin,D.1994.Spectrophotometric determination of vicinal glycols.Application to the determination of ribofuranosides.Analytical Chemistry,26,1092～1093.
183.Dong Q,Zheng LY.FangJN.1996.Study on the determination of ogosac-charide and polysaccharide bymodified phenol-sulfuracidmethod.中国中药杂志,31(9)：550．
184.Edge C J,Rademacher TW,Wormald MR.1992.Fast sequencing of oligosaccharides:the eagentarray analysis method.Proc.Natl.Acad.Sci,89(4):6338-6342.
185.Fran,Z G.1998.Polysaccharides inpharmacology.Currentapplicationandfuture con-cepts.PlantaMed,55(6):493-497.
186.G Prabhakar Reddy,Chi-Chou Chang,etal.Determinationby Heteronuclear NMR Spectroscopy of the CompleteStructure of the Cell Wall Polysaccharide of Streptococcussanguis Strain K103.Anal Chem,1993,65:913-921.
187.Gaio T,Ester C,Alberto B,etal.2002.Xanthanandglucomannanmix-tures:synergistic interactions and gelation.Bioma-cromo-lecules,(3):498-504.
188.Gomes,A.,Vedasiromoni,J.R.,Das,M.,Sharma,R.M.,Ganguly,D.K.1995.Antihyperglycemic effect of black tea (Camellia sinensis) in rat.Journal of Ethnopharmacology,45:223～226.
189.Hauke Hilz,Edwin J Bakx,Henk A Schols,etal.2005.Cell wallpolysaccharides in black currants and bilberries-characterization in berries,juice,andpresscake.Carbohydrate Polymers,58:477-488.
190.Hikno H,Ishiyama M,Suzuki Y,etal.1999.Mechanisms of hypoglycemic activity of gandodern B:a glycan of ganoderma lucidum fruit bodies.Planta Medica,,55(2):423-428.
191.Imsa,Kim K,Lee CK.2006.Immunomodulatory activity of polysaccharides isolated from Salicornia herbacea.Int Immunopharmcol,6(9):1451-1458.
192.Ishibashi K Miura NN Adachi Y etal.2001.Relationship between solubility of grifolan,a fungal 1,3-p-D-glucan,and production of tumor necrosis factor by macrophages in vitro.Biosci Biotechnol Biochem,65(9):1993～2000.
193.Ivy J L.1991.Musel glycogen synthesis before and after exercise.Sports Med,11(1):6-19.
194.Jagdzinski PP,Lewandowska M,Januchowski R,etal.2002.The effectofhigh molecular weight dextran sulfate on the production of interleukin-8 in monocyte cell culture.Biomed Phannacother,56(5)254-257.
195.Jimmy Muldoon etal.2002.Structure of two polysaccharides of Campylobacter jejuni 81116.Carbohydrate Research.,337,222.-2229.
196.JonLundqvist,AnitaTeleman,LindaJunel,etal.2002.Isolation and characterization of galac to glu comannan from spruce.Carbohydrate Polymers,48:29-39.
197.Kameswara Rao,B.Giri,R.,Kesavalu,M.M.Apparao,Ch.1997.Herbal medicine:in the management of diabetes mellitus.Manphar Vaidhya Patrika 33-35.
198.Kardosova,Machovae.2006.Antioxidant activity of medicinal plant polysac-charides.Fitoterapia,77(5):367-373.
199.Katherine D,McReynolds,JacquelynGervay-Hague.2000.Exa-mining the secondary structures of unnatural peptides and carbohydrate-based compounds utilizing circular dichrosism.Tetrahedron:A symmetry,11:337-362.
200.Khushbaktova Z A,Yusupova S M,Zamaraeva M V,etal.1996.Interrelationships of the stuctures and antioxidant activities of some flavonoids from the plant of central Asia.Chem Natural Compounds,32(3):338-340.
201.Kiho T,Kochi M,Usui S,etal.2001.Antidiabetic effect of anacidic polysaccharide(TAP) from Tremella aurantia and its degradation product.Biol Pharm Bull,24(12):1400-1403.
202.Kodama N,Harada N.2002.A polysaccharide,extract from Grifola frondosa,induces Th-1 dominant responses in carcinoma-bearing BALB/c mice.Jpn J Pharmacol,904,90(4):357-360.
203.Konno C,Sugiyama K,KanoM.1998.Isolation and hypoglycaemic activity of panaxans A,B,C,D,and E,glycans of Panax ginseng roots.Planta Med,50:434-436.
204.L.J.Miller,J.W.Blunt.2002.Evaluation of the structure of the Polysaccharides from Chondria macrocarpa and Ceramium rubrum as Determined by ~(13)CNMRspectroscopy.Botanica Marina,45,1-8.
205.Lamartiniere,Colal A.2000.Protection against breast cancer with genistein:a component of soy.Am.j.Clin.Nutr,71(6 suppl):1705-1706.
206.Lee B C,Bae JT,Pyoh B,etal.2003.Biological activities of the polysaccharides produced from submer gedculture of the edible BasidiomyceteGrifolafrondosa.EnzymeMicrobTechnol,32(5):574-51.
207.Li J,Han XH,Li ZH,etal.2000.Isolation and determination ofpachyman in Poriacocos(Schw)Wolf[J].Chin J Mod ApplPharm,17(1):49-50.
208.Luetti G B,Steinmuller C,Gifford G E,etal.1999.Macrophage activation by the polysaccharide arabinogalactan isolated from plant cell cultures of Echinaceapurpurea.JNalt Cancer Inst,81(9):669-675.
209.Matsumot O T,Cyong J C,Kiyohar A H,etal.1995.Regulationofimmune com-plexesbind-ingofmacrophages by pectic polysaccharide from Bupleurumfal-catumL.Pharmacological evidence for the requirement of intracellular calci-um/calmodulin on Fcreceptor up-regulation by bupleuran 2b.Journal of Pharmacy andPharmacology,47(2):152-156.
210.Matsumot OT,Kiyohar AH,Yamad AH,etal.1996.The pectic polysaccharide from Bupleurum falcatumL.enhances clearance of immune complexes in mice.PhytotherapyResearch,10(7):585-588.
211.Michael Miller,MD.2003.Raising an isolated low HDL-C levehWhy,how,and when?.Cleveland Clinic Journal of Medicine,6 (70):560.
212.Mock,K.K.etal,1991.The Analysis of Underivatized Oligosacchrides by Matrix-assisted Laser Desorption Mass Spectrometry,Biochem Biophys Res Comm,177(2):644-651.
213.Mota A.etal.1990.Struture-activity relationships of polymethxyflavones and other flavonoids as inhabitorys of non-enzymic lipid peroxidation.Biochem Pharmacol,40:793-797.
214.N.E.Awad.1994.Lipid content and antimicrobial activity of phenolic constituents of cultivated Portulaca Oleracea L.Bull.Fac.Pharm.Cairo Univ.,32 (1):137-142.
215.Naomi Sakai,Kyouko Inada.1996.Michi Odamoto.Portuloside A,a monoterpene glucoside from portulaca oleracea.Phytochemistry,42 (6):1625-1628.
216.Nicola,Volpi.2003.Milligram-scale preparation and purificationof oligosaccharides of defined length possessing thestructure of chondroitin from defructosylated capsularpolysaccharide K4.Glycobiology,13(9):635-640.
217.Ourdia Bouzid,David Navarro,Marjolaine Roche,etal.2005.Fungal enzymes as a powerful tool to release simple phenoliccompounds from olive oil by-products.Process Biochemistry,40:1855-1862.
218.Oussama etc.2002.Fungal cell-wall galactomannans isolated from Geotrichum spp.And their teleomorphs,Dipodascus and Galactomyces.Carbohydrate Research.337:2347-2351.
219.P.Jackson.1994.The analysis of fluorophore-labelled glycans by high-resolution lyacrylamide gel eleterophoresis.Anal Biochem,216(2):243-252.
220.P Valachovi,A Pechova,T J Mason.2001.Towards theindustrial production of medicinaltincture by ultrasound assistedextr action.Ultrason.Sonochem,(8):111-117.
221.Peng Zhi-ying,Zhang Ning,Zhong Yan-lin.2002.Molecular Structure of Microbial Exo polysaccharides PS-9415.Journal of South China University of Technology,30 (3):78-82.
222.PigeoletE.1990.GSH-Px,SOD and CAT inactivation by peroxidesOxgen derived free radicals.MechAgeingDev,50:283-297.
223.Pinto GLD,Martinez M,Corredor ALD,etal.1996.Structural studies of the polysaccharide isolated from Spondias purpurea gum exudates.Carbohydrate Res,290:97-103.
224.Qin Chuanguang,Huang Kaixun,Xu Huibi.2002.Isolation and characterization of a novel polysaccharide from the mucus of the loach,Misgurnus anguillicaudatus.Carbohydrate polymers,49:367-371.
225.Roman-Ramos,R.,Flores-Saenz,J.L.Alarcon-Aguilar,F.J.1995.Antihyperglycemic effect of some edible plants.Journal of Ethnopharmacology,48,25-32.
226.SaitoH,Yoshioka Y,Aketagawa NUJ,etal.1997.Relationship between conformation and biological response for(1→3)-β-D-glucans in theactivation ofcoagulation Factor G from limulus amebocyte lysate andhost-mediated antitumor activity.Demonstration of single-helixconformation as a stimulant.Carbohydrate Research.217:181-190.55.
227.Saul Roseman.2001.Reflections on Glycobiology,JBC,276(45):41527-41542.
228.SlavomirBystricky,ShousunCSzu,etal.1995.circular dich-roism of the o-specific polysaccharide of vibrio choleraeol and some related derivatives.Carbohyydrate research,270:115-122.
229.Sofya N.Senchenkova,etal.2000.Structure of the O-specific polysaccharides of the lippo polysaccharides of Xanthomonas campestris PV.vignicola GSPB 2795 and GSPB 2796.Carbohydrate Research,329,329:831-838.
230.Stimpelm,Prokscha,Wagnerh,etal.1994.Macrophage activation and in-duction ofmacrophage cytotoxicity by purified polysaccharide fractions fromtheplantEchinaceapurpurea.Infect Immun,46(3):845-849.
231.Susumu Honda,Shigeo Suzuki,etal.2003.Analysis of carbohyd-rates as 1-pheny 1-3-methy 1-5-pyrazolone derivatives by capillary/microchip electrophoresis andcapillaryelectro-chromatography.JournalofPharmaceuticalandBiomedicalAnalysis,30:1689-1714.
232.Szkudelski T.2001.The mechanism of alloxan and streptozotocin action in p cells of the rat pancreas.Physiol Res,50:536-546.
233.Tang W,Heman I,Bertram B.2003.Recent development of antitumor agents from Chinese herbal medicines.Partll.High molecular lompounds.Panta Med,69:193-201..
234.Taylor,R.L.,Conrad,H.E.1992.Stoichiometric depolymerization of polyuronides and glycosamino glycuronans to monosaccharides following reduction of their carbodiimide-activated carboxyl groups.Biochemistry,11,1383—1388.
235.Unursaikhan Surenjav,Lina Zhang,Xiaojuan Xu,etal.2006.Effects of molecular structure on antitumor activities of (1—>3)-p-D-glucan from different Lentinus Edodes.Car-bohydrate Polymers.63(63):97-104.
236.V.N Reinhold,B.B.Reinhold,S.Chan.1996.Carbohydrate sequence analysis by electrospray ionization mass spectrometry.Methods enzymol,271:377-402.
237.W.S.Hancock,A.Appffel,etal.1996.Reversed-phase peptide mapping of glycoprotcins using liquid chromatography/eletrospray ionization-mass spectrometry.Methods Enzymol,271:403-427.
238.Wang,H.X.,Ng,T.B.1999.Natural products with hypoglycemic,hypotensive,hypocholesterolemic,antiatherosclerotic and antithrombotic activities.Life Sciences,65:2663～ 2677.
239.Wang,Z.J.,Luo,D.H.,& Liang,Z.Y.2004.Structure of polysaccharides from the fruiting body of Hericium erinaceus Pers.Carbohydrate Polymers,57,241-247.
240.Wang J H,Wang S J,Wei W C,etal.2004.Use of plant Polysaccharidesas topi-cally applied adjuvants for enhancing immune responses viaTransdermal Gene Vaccination.MolTher,9(S1):207.
241.Wermurh C.G 1992.Meditional Che mistry for the 21st century (IUPAC Che mistry for the 21~(st) Century),London:Blackwell Scientific Publications,38-84..
242.Whitworth W,Charles C P,Joseph E,etal.1990.Macrophages and cancer,Cancer and Metasis,319-322.
243.Xiang D B,Li X Y.1993.Antitumor actity and immuno-potentiating actions of Achyanthes bidentata polysaccharides.Acta Pharmacol.Sin,14(6):556-561.
244.Xu Jing,Loo George.2001.Different effects of genistein on molecular markers related to apoptosis in two phenotypically dissimilar breast cancer line.Cell Biochem,82(1):78-88.
245.XuZH,ZhouSW.2001.Progress of plant polysaccharides and its hypoglycemiceffects.West China J Pharm Sci,16(5):365-366.
246.Yadav S,Vats V,Dhunnoo Y,Grover JK.2002.Hypoglycemic and antihyperglycemic activity of Murraya koenigii leaves in diabetic rats.J Ethnopharmacol,82:111 ～ 116.
247.Yoon SJ,Pereira MS,Pavaom SG,etal.2002.The medicinal plant Poranavolubiliscontains polysaccharides with anticoagulant activity mediated by hep-arin cofactor Ⅱ.ThrombRes, 106(1): 59-62.
248. ZHrom(?)dkov(?), A Ebringerov(?), P Valachovi. 2002. Ultrasound-assisted extraction of water-soluble polysaccharides from theroots of valerian. Ultrason Sonochem, (9):37-44.
249. ZHrom(?)dkov(?), AEbringerov(?), PValachovi(?). 1999. Comparison of classicalandultrasound-assisted extraction of polysaccharides from Salvia officinalis L. Ultrason Sonochem, (5): 163-168.