针灸对运动应激大鼠免疫机能的调节作用
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摘要
目的:
观察针灸对长期剧烈运动应激状态下大鼠免疫功能的调节作用,并从神经-内分泌-免疫网络(HPA轴)的途径深入探讨针灸发挥作用的机制。
方法:
清洁级8周龄健康雄性SD大鼠59只,按数字随机分为四组:对照组(n=10),大强度游泳训练组(n=17),大强度游泳训练加艾灸组(n=16),大强度游泳训练加电针艾灸组(n=16)。以长期(8周)大强度(150min)游泳训练建立运动性免疫抑制大鼠模型,选用关元、百会、足三里三穴,采取单纯艾灸关元、百会、足三里三穴与电针双侧足三里穴加艾灸关元、百会穴的方式观察针灸干预对长期大强度运动应激状态下大鼠免疫机能的影响,并从神经—内分泌—免疫网络的角度深入探讨针灸干预作用的机制。运用放射免疫法测定大鼠下丘脑ACTH、CRH、CORT及IL-2的浓度;运用流式细胞仪技术测定大鼠T淋巴细胞亚群CD3~+、CD4~+、CD8~+、CD161~+(NK细胞)的数量;运用ELISA法测定大鼠血清SIL-2R、γ-IFN的浓度;运用~3H-TdR渗入法测试大鼠血淋巴细胞对有丝分裂原PHA的体外增殖能力。
结果:
与对照组比,大强度训练组的IL-2、γ-IFN显著性下降(P＜0.01,P＜0.05),NK细胞也有下降趋势(P＞0.05),提示8周大强度游泳训练导致大鼠IL-2-IFN-NKC免疫调节网出现紊乱;尽管艾灸+大强度训练组的IL-2、γ-IFN、NK细胞均低于对照组,但IL-2没有统计学意义(P＞0.05),同时与大强度训练组相比较,艾灸+大强度训练组的γ-IFN、IL-2在数值上仍高于大强度训练组;尽管电针+艾灸+大强度训练组IL-2、γ-IFN、NK细胞的也均出现下降,但与对照组比均无统计学意义(P＞0.05),且其γ-IFN在数值上仍显著高于大强度训练组(P＜0.05)。针灸各组间比较,艾灸+大强度训练组的γ-IFN、NK细胞低于电针+艾灸+大强度训练组,但无统计学意义(P＞0.05)
与对照组比,大强度训练组的大鼠体重显著性下降(P＜0.01),SIL-2R却显著性升高(P＜0.05),而艾灸+大强度训练组与电针+艾灸+大强度训练组的大鼠体重尽管也显著地低于对照组(P＜0.01),但在数值上仍高于大强度训练组(P＞0.05),同时,针灸二组的SIL-2R不仅均非常显著性低于大强度训练组(P＜0.01),而且均显著性低于对照组(P＜0.01)。
与对照组比,大强度训练组的CD3~+、CD4~+/CD8~+比值、NK细胞均出现下降,其中CD3~+具有显著性意义(P＜0.05),而其CD4~+尽管略有升高,但因其CD8~+也出现升高,故其CD4~+/CD8~+的比值仍有下降趋势(P＞0.05);而艾灸+大强度训练组的CD3~+、CD4~+、CD4~+/CD8~+的比值均出现升高,其中CD4~+具有显著性意义(P＜0.05),同时,CD3~+在数值上非常显著高于大强度训练组(P＜0.01),但艾灸+大强度训练组的NK细胞出现具有统计学意义(P＜0.05)的下降;电针+艾灸+大强度训练组的T淋巴细胞亚群各指标除NK细胞略有下降(P＞0.05)外,其余均出现升高,其中CD3~+、CD4~+具有非常显著性意义(P＜0.01,P＜0.01),同时,电针+艾灸+大强度训练组的T淋巴细胞亚群各指标在数值上均高于大强度训练组,其中CD3~+、CD4~+具有显著性意义(P＜0.01,P＜0.05)。
与对照组比,大强度训练组的脾脏指数、胸腺指数、淋巴细胞增殖能力均显著性下降(P＜0.01,P＜0.01,P＜0.05),而艾灸+大强度训练组与电针+艾灸+大强度训练组的三项指标尽管也有下降趋势,但均无统计学意义(P＞0.05),同时,在数值上,针灸二组的脾脏指数、胸腺指数仍非常显著高于大强度训练组(P＜0.01)。
与对照组相比,大强度训练组的CRH、ACTH、CORT均出现升高,其中ACTH、CORT具有显著性意义(P＜0.01,P＜0.05),而艾灸+大强度训练组的CRH、CORT有下降趋势,但无显著性意义(P＞0.05),尽管艾灸+大强度训练组的ACTH显著高于对照组(P＜0.01),但与大强度训练组相比较,其CRH、ACTH、CORT均出现降低,其中ACTH、CORT具有非常显著性意义(P＜0.01);电针+艾灸+大强度训练组的CRH、ACTH、CORT与对照组相比较,CRH、ACTH略有升高,而CORT却有下降趋势,但均无显著性意义(P＞0.05),而与大强度训练组相比较,电针+艾灸+大强度训练组的CRH、ACTH、CORT均出现降低,其中ACTH、CORT也具有非常显著性意义(P＜0.01);针灸各组间比较,电针+艾灸+大强度训练组的CRH、CORT略高于艾灸+大强度训练组,但无显著性意义(P＞0.05),而电针+艾灸+大强度训练组的ACTH显著低于艾灸+大强度训练组(P＜0.05)。
1、经过8周大强度游泳应激后,大鼠的神志、外观、体重等一般状况恶化,大鼠血液中的主要免疫效应指标IL-2、γ-IFN、NKC、T淋巴细胞亚群中的CD3~+、CD4~+、CD4~+/CD8~+的比值及大鼠脾脏指数、胸腺指数、血淋巴细胞对有丝分裂原PHA的体外增殖能力均出现显著性下降,而免疫抑制因子SIL-2R含量、HPA轴中CRH、ACTH、CORT均出现显著性升高。
2、单纯艾灸百会、关元、足三里穴不仅可提高CD3~+的百分率,而且可显著提高辅助性T细胞CD4~+的百分率、下调抑制性T细胞CD8~+的浓度,引起CD4~+/CD8~+的比值升高,同时可以一定程度地阻止长期大强度运动应激状态下大鼠胸腺指数、脾脏指数及血淋巴细胞对有丝分裂原PHA的体外增殖反应的下降,但对长期大强度运动应激状态下NK细胞数量的下降缺乏正向调节作用。艾灸对长期大强度运动应激状态下IL-2-IFN-NKC免疫调节网的正向调节作用也比较有限,但仍可一定程度地阻止IL-2的下降。
3、电针双侧足三里穴加艾灸百会、关元穴不仅可以有效下调NK细胞下降的幅度、升高CD4~+/CD8~+的比值,而且对CD3~+、CD4~+具有明显的促进作用,同时也可有效地阻止长期大强度运动应激状态下大鼠的胸腺指数、脾脏指数及血淋巴细胞对有丝分裂原PHA的体外增殖反应的下降。对长期大强度运动应激状态下IL-2-IFN-NKC免疫调节网的正向调节作用较单纯的艾灸更为全面,其可以有效地阻止大鼠IL-2、γ-IFN及NK细胞数量的下降。
4、无论是单纯的艾灸还电针+艾灸的方式均可通过下调ACTH、CORT和CRH的含量,从3个层次对HPA轴的激素进行调节,改善HPA轴的功能,避免HPA轴功能持续过度亢进,从而实现对因长期剧烈运动应激引起的神经内分泌紊乱的良性干预作用。
结论:
1、长期大强度运动应激不仅可以导致大鼠的神经、外观、体重等一般情况恶化,同时可引起大鼠免疫器官结构萎缩、功能退化、免疫细胞数量减少、免疫分子分泌抑制,IL-2-IFN-NKC免疫调节网紊乱,表明大鼠出现运动性免疫抑制。而长期大强度运动应激引起HPA轴功能持续过度亢进,导致大鼠体内CORT过量合成,从而导致神经内分泌免疫功能紊乱,可能是导致机体免疫功能出现抑制(运动性免疫抑制)的主要原因。
2、针灸可以减轻长期而剧烈的运动应激对机体免疫功能的损害,有效避免机体出现运动性免疫抑制,其作用的机制一方面可能是通过纠正HPA轴的功能紊乱,进而下调CORT的浓度来实现对机体在长期大强度运动应激状态下免疫功能的保护效应,有效地避免了运动性免疫抑制的发生;另一方面也可能与直接调整紊乱的IL-2-IFN-NKC免疫调节网进而良性调节免疫机能存在密切联系。
3、针灸对长期大强度运动应激状态下大鼠紊乱的HPA轴功能的调节作用是多途径,多层次的,不同的针灸方式发挥良性干预作用的途径也可能存在差异,本次研究结果显示:针+灸的方式对大鼠免疫机能的调节作用较单纯的艾灸更为全面。
Objective:To study the influence that the Acupuncture pay on the mouse of long-term acute sport,and probe into the mechanism of Acupuncture nerve-internal secretion-immunity network(HPA).
Methods:59 Clean 8-week-old healthy male SD rats,according to numbers,were randomly divided into four groups:control group(n=10),high-intensity swimming training group(n=17),the group of high-intensity swimming training with Moxibustion and Acupuncture(n=16),the group of high-intensity swimming training with moxibustion and electro-Acupuncture(n=16).Long-term(8 weeks) high-intensity (150min) swimming training exercise-induced immunosuppression to establish the rat model,select the three points of Guanyuan,Baihui,Zusanli,put simple moxibustionon three points of Guanyuan,Baihui,Zusanli,and put electro-Acupuncture on the point of Zusanli while put Moxibustion on the points of Guanyuan,Baihui,and then observe Acupuncture intervention on long-term high-intensity exercise stress state of the immune function of rats.From the way of nerve-endocrine-immune network,probe into the mechanism of Acupuncture intervention.Measured by radioimmunoassay using rat hypothalamic ACTH,CRH,CORT and IL-2 concentration;the use of flow cytometry determination of rat T-lymphocyte subsets CD3~+、CD4~+、CD8~+、CD161~+(NK cells) number; use ELISA determination of serum SIL-2R,γ-IFN concentration;the use of 3H-tdr infiltration method of testing blood lymphocyte mitogen in vitro proliferation of PHA.
Results:
Comparing to the control group,the IL-2,γ-IFN of the high-intensity training group decreased significantly((P＜0.01,P＜0.05),NK cells are on a downward trend(P＞0.05), which imply that the eight-weeks high-intensity swimming training resulted in rats'IL-2, IFN-NKC immunoregulatory network disorder occur;Although the IL-2,γ-IFN,NK cells of the group of high-intensity training with Moxibustion are lower than that of the control group,but IL-2 is not statistically significant(P＞0.05).Compared to the group of the high-intensity training,the values of theγ-IFN,IL-2 of the group of high-intensity training with moxibustionare still higher than that of high-intensity training group;Although the IL-2,γ-IFN,NK cells of the group of high-intensity training with electro-Acupuncture and moxibustion also decline,but it is not of statistical significance(P＞0.05).Comparing to control group,itsγ-IFN in the value still significantly higher than that of the high-intensity training group(P＜0.05).Comparing each other,theγ-IFN,NK cells of the group of high-intensity training with Acupuncture and moxibustion+ are less than that of the high-intensity training with electro-Acupuncture and moxibustion,but no statistical significance(P＞0.05).
Comparing to the control group,the weighs of the rats of the group of the high-intensity training decreased significantly(P＜0.01),the SIL-2R is significantly higher(P＜0.05), and the weighs of the rats of the group of the high-intensity training with electro-Acupuncture and Moxibustion are significantly lower than the control group (P＜0.01),but still higher than that of high-intensity training group(P＞0.05),the SIL-2R of the two groups with Acupuncture is not only significantly lower than that of the high-intensity training group(P＜0.01),but were significantly lower than that of the control group(P＜0.01)。
Comparing to the control group,CD3~+、the ratio of CD4~+/CD8~+,and the NK cells of the high-intensity training group decrease significantly(P＜0.05),while CD4~+ increase slightly,but CD8~+ also increased,so the ratio of CD4~+/CD8~+ still downward trend(P＞0.05);CD3~+、CD4~+、the ratio of CD4~+/CD8~+ of the group of high-intensity training with Moxibustion increased in both,in which CD4~+ with significance(P＜0.05),CD3~+ is very significantly higher than that of high-intensity training group(P＜0.01),but NK cells of the group of high-intensity training with Moxibustion decrease significantly(P＜0.05);The T-lymphocyte subsets of the indicators except the NK cells of the group of high-intensity training with lectro-Acupuncture and Moxibustion decreased slightly(P＞0.05),the remaining increase in both,including CD3~+、CD4~+ with a very significant significance (P＜0.01,P＜0.01),the indexes of the T-lymphocyte subsets of the group of high-intensity training with electro-Acupuncture and Moxibustion are higher than that of high-intensity training group,CD3+,CD4+ are of significance(P＜0.01,P＜0.05).
Comparing to the control group,the spleen index,thymus index,the proliferation ability of lymphocytes of the high-intensity training group were significantly reduced(P＜0.01, P＜0.01,P＜0.05),and the three indexes of the group of the high-intensity training group with Moxibustion and electro-Acupuncture and the group of high-intensity training with Moxibustion are on a downward trend though,but no statistical significance(P＞0.05),in the numerical,the spleen index and thymus index of the two groups with Acupuncture are significantly higher than that of high-intensity training group(P＜0.01).
Comparing to the control group,the CRH,ACTH,CORT of the high-intensity training group increased all,in which ACTH,CORT are significant(P＜0.01,P＜0.05),and the CRH,CORT of the group of high-intensity training with Moxibustion has been a downward trend,but no significant(P＞0.05),despite the ACTH of the group of high-intensity training with Moxibustion significantly higher than that of the control group (P＜0.01),but comparing to the group of high-intensity training the CRH,ACTH,CORT reduced all,in which ACTH,CORT is very significant(P＜0.01) statistically;Compared to the control group,CRH,ACTH of the group of high-intensity training with electro-Acupuncture and Moxibustion slightly increased,and CORT is downward trend, but they are not significant(P＞0.05),compared to the group of high-intensity training group,the CRH,ACTH and CORT of the group of high-intensity training with electro-Acupuncture and Moxibustion reduce all,in which ACTH,CORT are significant (P＜0.01) statistically.Comparing each other amongst Acupuncture groups,the CRH, CORT of the group of high-intensity training with electro-Acupuncture and Moxibustion are slightly higher than that of the group of high-intensity training with moxibustion,but it is not significant statistically(P＞0.05),the ACTH of the group of high-intensity training with Moxibustion and electro-Acupuncture are significantly lower than that of the group of high-intensity training with moxibustion(P＜0.05).
1.After high-intensity swimming stress of eight weeks,mind,appearance,body weight of rats are deteriorated,the rats' blood immune indexes:IL-2,γ-IFN,NKC,CD3~+、CD4~+、ratio of CD4~+/CD8~+ of T-lymphocyte subsets,and the spleen index,thymus index,blood lymphocytes to mitogen in vitro proliferation of PHA showed a significant decrease,while the immunosuppressive factor SIL-2R concentration,HPA axis in CRH,ACTH,CORT were significantly increased.
2.Simply putting moxibustionon Baihui,Guanyuan,Zusanli not only can improve the percentage of CD3~+,and can significantly increase the percentage of CD4~+ of T helper cells,reduced concentration of CD8~+ of suppressive T cells,caused the ratio of CD4~+/CD8~+ increased,to some extent can prevent the decline of proliferative response of thymus index,spleen index and blood lymphocytes to PHA mitogen under the state of long-term high-intensity exercise stress,but decline of NK cells' quantity lack of positive regulatory role under the state of long-term high-intensity exercise stress.Moxibustionplay limited regulatory role on IL-2-IFN-NKC immunoregulatory network,it can still prevent to some extent the decline of IL-2.
3.Electro-Acupuncture on the points of Bilateral Zusanli and Moxibustion on the points of Guanyuan and Baihui can not only effectively reduced the decrease in NK cells,increased the ratio of CD4~+/CD8~+,but also promote CD3~+、CD4~+ with a significant role,can also effectively prevent the decline vitro proliferative response of thymus index,spleen index and blood lymphocytes to PHA mitogen.o rats under the state of long-term high-intensity exercise stress.They can play a more comprehensive positive role on adjusting IL-2-IFN-NKC immunoregulatory network than simply Moxibustion under the state of long-term high-intensity exercise stress,which can effectively prevent decline in the number of IL-2,γ-IFN and NK cells of the rats.
4 Whether simple Moxibustion or electro-Acupuncture combined with Moxibustioncan regulate the hormone of the HPA axis from the three levels,improve the function of HPA axis to avoid HPA axis function sustained over-hyper,through reducing ACTH,CORT and CRH content.It can achieve a benign intervention role disorders of the neuroendocrinef induced by long-term strenuous exercise stress.
Conclusions:
1 Long-term high-intensity exercise stress can not only lead to the nerve appearance, body weight of rats deteriorated,and can cause shrinkage of the structure of immune organs of rats,the functional degradation,reduction in the number of immune cells,inhibit secretion of immune molecules,IL-2-IFN-NKC immunoregulatory network disorder,show that exercise induced rats immunosuppression.And long-term high-intensity exercise stress cause the continued excessive HPA axis hyperfunction,resulting in excessive synthesis of CORT rats,and then resulting in neuroendocrine-immune dysfunction,which may be the main reasons of leading to emergence of immune function suppression(exercise-induced immune suppression)
2 Acupuncture can reduce the long-term strenuous exercise stress on the immune function of the damage,and can effectively prevent exercise-induced immunosuppression,its mechanism may be:on the one hand,correcting dysfunction of the HPA axis,and then down the concentration of CORT to achieve the protective effect of immune function of the body of long-term high-intensity exercise stress state,and effectively prevent the movement of the occurrence of immunosuppression;on the other hand,related to directly adjustment disorder of IL-2-IFN-NKC immunoregulatory network and then positive regulation of closely linked immunodeficiency.
3 the regulation that Acupuncture pay on HPA axis function disorder of rat of long-term high-intensity exercise stress of rats is multi-channel and multi-level,different methods of Acupuncture intervention play a positive role differently,the results of this study show: Acupuncture combined with Moxibustion pay more comprehensive adjustment on the immune function of rats than the simple Moxibustion.
观察针灸对长期剧烈运动应激状态下大鼠免疫功能的调节作用,并从神经-内分泌-免疫网络(HPA轴)的途径深入探讨针灸发挥作用的机制。
方法:
清洁级8周龄健康雄性SD大鼠59只,按数字随机分为四组:对照组(n=10),大强度游泳训练组(n=17),大强度游泳训练加艾灸组(n=16),大强度游泳训练加电针艾灸组(n=16)。以长期(8周)大强度(150min)游泳训练建立运动性免疫抑制大鼠模型,选用关元、百会、足三里三穴,采取单纯艾灸关元、百会、足三里三穴与电针双侧足三里穴加艾灸关元、百会穴的方式观察针灸干预对长期大强度运动应激状态下大鼠免疫机能的影响,并从神经—内分泌—免疫网络的角度深入探讨针灸干预作用的机制。运用放射免疫法测定大鼠下丘脑ACTH、CRH、CORT及IL-2的浓度;运用流式细胞仪技术测定大鼠T淋巴细胞亚群CD3~+、CD4~+、CD8~+、CD161~+(NK细胞)的数量;运用ELISA法测定大鼠血清SIL-2R、γ-IFN的浓度;运用~3H-TdR渗入法测试大鼠血淋巴细胞对有丝分裂原PHA的体外增殖能力。
结果:
与对照组比,大强度训练组的IL-2、γ-IFN显著性下降(P＜0.01,P＜0.05),NK细胞也有下降趋势(P＞0.05),提示8周大强度游泳训练导致大鼠IL-2-IFN-NKC免疫调节网出现紊乱;尽管艾灸+大强度训练组的IL-2、γ-IFN、NK细胞均低于对照组,但IL-2没有统计学意义(P＞0.05),同时与大强度训练组相比较,艾灸+大强度训练组的γ-IFN、IL-2在数值上仍高于大强度训练组;尽管电针+艾灸+大强度训练组IL-2、γ-IFN、NK细胞的也均出现下降,但与对照组比均无统计学意义(P＞0.05),且其γ-IFN在数值上仍显著高于大强度训练组(P＜0.05)。针灸各组间比较,艾灸+大强度训练组的γ-IFN、NK细胞低于电针+艾灸+大强度训练组,但无统计学意义(P＞0.05)
与对照组比,大强度训练组的大鼠体重显著性下降(P＜0.01),SIL-2R却显著性升高(P＜0.05),而艾灸+大强度训练组与电针+艾灸+大强度训练组的大鼠体重尽管也显著地低于对照组(P＜0.01),但在数值上仍高于大强度训练组(P＞0.05),同时,针灸二组的SIL-2R不仅均非常显著性低于大强度训练组(P＜0.01),而且均显著性低于对照组(P＜0.01)。
与对照组比,大强度训练组的CD3~+、CD4~+/CD8~+比值、NK细胞均出现下降,其中CD3~+具有显著性意义(P＜0.05),而其CD4~+尽管略有升高,但因其CD8~+也出现升高,故其CD4~+/CD8~+的比值仍有下降趋势(P＞0.05);而艾灸+大强度训练组的CD3~+、CD4~+、CD4~+/CD8~+的比值均出现升高,其中CD4~+具有显著性意义(P＜0.05),同时,CD3~+在数值上非常显著高于大强度训练组(P＜0.01),但艾灸+大强度训练组的NK细胞出现具有统计学意义(P＜0.05)的下降;电针+艾灸+大强度训练组的T淋巴细胞亚群各指标除NK细胞略有下降(P＞0.05)外,其余均出现升高,其中CD3~+、CD4~+具有非常显著性意义(P＜0.01,P＜0.01),同时,电针+艾灸+大强度训练组的T淋巴细胞亚群各指标在数值上均高于大强度训练组,其中CD3~+、CD4~+具有显著性意义(P＜0.01,P＜0.05)。
与对照组比,大强度训练组的脾脏指数、胸腺指数、淋巴细胞增殖能力均显著性下降(P＜0.01,P＜0.01,P＜0.05),而艾灸+大强度训练组与电针+艾灸+大强度训练组的三项指标尽管也有下降趋势,但均无统计学意义(P＞0.05),同时,在数值上,针灸二组的脾脏指数、胸腺指数仍非常显著高于大强度训练组(P＜0.01)。
与对照组相比,大强度训练组的CRH、ACTH、CORT均出现升高,其中ACTH、CORT具有显著性意义(P＜0.01,P＜0.05),而艾灸+大强度训练组的CRH、CORT有下降趋势,但无显著性意义(P＞0.05),尽管艾灸+大强度训练组的ACTH显著高于对照组(P＜0.01),但与大强度训练组相比较,其CRH、ACTH、CORT均出现降低,其中ACTH、CORT具有非常显著性意义(P＜0.01);电针+艾灸+大强度训练组的CRH、ACTH、CORT与对照组相比较,CRH、ACTH略有升高,而CORT却有下降趋势,但均无显著性意义(P＞0.05),而与大强度训练组相比较,电针+艾灸+大强度训练组的CRH、ACTH、CORT均出现降低,其中ACTH、CORT也具有非常显著性意义(P＜0.01);针灸各组间比较,电针+艾灸+大强度训练组的CRH、CORT略高于艾灸+大强度训练组,但无显著性意义(P＞0.05),而电针+艾灸+大强度训练组的ACTH显著低于艾灸+大强度训练组(P＜0.05)。
1、经过8周大强度游泳应激后,大鼠的神志、外观、体重等一般状况恶化,大鼠血液中的主要免疫效应指标IL-2、γ-IFN、NKC、T淋巴细胞亚群中的CD3~+、CD4~+、CD4~+/CD8~+的比值及大鼠脾脏指数、胸腺指数、血淋巴细胞对有丝分裂原PHA的体外增殖能力均出现显著性下降,而免疫抑制因子SIL-2R含量、HPA轴中CRH、ACTH、CORT均出现显著性升高。
2、单纯艾灸百会、关元、足三里穴不仅可提高CD3~+的百分率,而且可显著提高辅助性T细胞CD4~+的百分率、下调抑制性T细胞CD8~+的浓度,引起CD4~+/CD8~+的比值升高,同时可以一定程度地阻止长期大强度运动应激状态下大鼠胸腺指数、脾脏指数及血淋巴细胞对有丝分裂原PHA的体外增殖反应的下降,但对长期大强度运动应激状态下NK细胞数量的下降缺乏正向调节作用。艾灸对长期大强度运动应激状态下IL-2-IFN-NKC免疫调节网的正向调节作用也比较有限,但仍可一定程度地阻止IL-2的下降。
3、电针双侧足三里穴加艾灸百会、关元穴不仅可以有效下调NK细胞下降的幅度、升高CD4~+/CD8~+的比值,而且对CD3~+、CD4~+具有明显的促进作用,同时也可有效地阻止长期大强度运动应激状态下大鼠的胸腺指数、脾脏指数及血淋巴细胞对有丝分裂原PHA的体外增殖反应的下降。对长期大强度运动应激状态下IL-2-IFN-NKC免疫调节网的正向调节作用较单纯的艾灸更为全面,其可以有效地阻止大鼠IL-2、γ-IFN及NK细胞数量的下降。
4、无论是单纯的艾灸还电针+艾灸的方式均可通过下调ACTH、CORT和CRH的含量,从3个层次对HPA轴的激素进行调节,改善HPA轴的功能,避免HPA轴功能持续过度亢进,从而实现对因长期剧烈运动应激引起的神经内分泌紊乱的良性干预作用。
结论:
1、长期大强度运动应激不仅可以导致大鼠的神经、外观、体重等一般情况恶化,同时可引起大鼠免疫器官结构萎缩、功能退化、免疫细胞数量减少、免疫分子分泌抑制,IL-2-IFN-NKC免疫调节网紊乱,表明大鼠出现运动性免疫抑制。而长期大强度运动应激引起HPA轴功能持续过度亢进,导致大鼠体内CORT过量合成,从而导致神经内分泌免疫功能紊乱,可能是导致机体免疫功能出现抑制(运动性免疫抑制)的主要原因。
2、针灸可以减轻长期而剧烈的运动应激对机体免疫功能的损害,有效避免机体出现运动性免疫抑制,其作用的机制一方面可能是通过纠正HPA轴的功能紊乱,进而下调CORT的浓度来实现对机体在长期大强度运动应激状态下免疫功能的保护效应,有效地避免了运动性免疫抑制的发生;另一方面也可能与直接调整紊乱的IL-2-IFN-NKC免疫调节网进而良性调节免疫机能存在密切联系。
3、针灸对长期大强度运动应激状态下大鼠紊乱的HPA轴功能的调节作用是多途径,多层次的,不同的针灸方式发挥良性干预作用的途径也可能存在差异,本次研究结果显示:针+灸的方式对大鼠免疫机能的调节作用较单纯的艾灸更为全面。
Objective:To study the influence that the Acupuncture pay on the mouse of long-term acute sport,and probe into the mechanism of Acupuncture nerve-internal secretion-immunity network(HPA).
Methods:59 Clean 8-week-old healthy male SD rats,according to numbers,were randomly divided into four groups:control group(n=10),high-intensity swimming training group(n=17),the group of high-intensity swimming training with Moxibustion and Acupuncture(n=16),the group of high-intensity swimming training with moxibustion and electro-Acupuncture(n=16).Long-term(8 weeks) high-intensity (150min) swimming training exercise-induced immunosuppression to establish the rat model,select the three points of Guanyuan,Baihui,Zusanli,put simple moxibustionon three points of Guanyuan,Baihui,Zusanli,and put electro-Acupuncture on the point of Zusanli while put Moxibustion on the points of Guanyuan,Baihui,and then observe Acupuncture intervention on long-term high-intensity exercise stress state of the immune function of rats.From the way of nerve-endocrine-immune network,probe into the mechanism of Acupuncture intervention.Measured by radioimmunoassay using rat hypothalamic ACTH,CRH,CORT and IL-2 concentration;the use of flow cytometry determination of rat T-lymphocyte subsets CD3~+、CD4~+、CD8~+、CD161~+(NK cells) number; use ELISA determination of serum SIL-2R,γ-IFN concentration;the use of 3H-tdr infiltration method of testing blood lymphocyte mitogen in vitro proliferation of PHA.
Results:
Comparing to the control group,the IL-2,γ-IFN of the high-intensity training group decreased significantly((P＜0.01,P＜0.05),NK cells are on a downward trend(P＞0.05), which imply that the eight-weeks high-intensity swimming training resulted in rats'IL-2, IFN-NKC immunoregulatory network disorder occur;Although the IL-2,γ-IFN,NK cells of the group of high-intensity training with Moxibustion are lower than that of the control group,but IL-2 is not statistically significant(P＞0.05).Compared to the group of the high-intensity training,the values of theγ-IFN,IL-2 of the group of high-intensity training with moxibustionare still higher than that of high-intensity training group;Although the IL-2,γ-IFN,NK cells of the group of high-intensity training with electro-Acupuncture and moxibustion also decline,but it is not of statistical significance(P＞0.05).Comparing to control group,itsγ-IFN in the value still significantly higher than that of the high-intensity training group(P＜0.05).Comparing each other,theγ-IFN,NK cells of the group of high-intensity training with Acupuncture and moxibustion+ are less than that of the high-intensity training with electro-Acupuncture and moxibustion,but no statistical significance(P＞0.05).
Comparing to the control group,the weighs of the rats of the group of the high-intensity training decreased significantly(P＜0.01),the SIL-2R is significantly higher(P＜0.05), and the weighs of the rats of the group of the high-intensity training with electro-Acupuncture and Moxibustion are significantly lower than the control group (P＜0.01),but still higher than that of high-intensity training group(P＞0.05),the SIL-2R of the two groups with Acupuncture is not only significantly lower than that of the high-intensity training group(P＜0.01),but were significantly lower than that of the control group(P＜0.01)。
Comparing to the control group,CD3~+、the ratio of CD4~+/CD8~+,and the NK cells of the high-intensity training group decrease significantly(P＜0.05),while CD4~+ increase slightly,but CD8~+ also increased,so the ratio of CD4~+/CD8~+ still downward trend(P＞0.05);CD3~+、CD4~+、the ratio of CD4~+/CD8~+ of the group of high-intensity training with Moxibustion increased in both,in which CD4~+ with significance(P＜0.05),CD3~+ is very significantly higher than that of high-intensity training group(P＜0.01),but NK cells of the group of high-intensity training with Moxibustion decrease significantly(P＜0.05);The T-lymphocyte subsets of the indicators except the NK cells of the group of high-intensity training with lectro-Acupuncture and Moxibustion decreased slightly(P＞0.05),the remaining increase in both,including CD3~+、CD4~+ with a very significant significance (P＜0.01,P＜0.01),the indexes of the T-lymphocyte subsets of the group of high-intensity training with electro-Acupuncture and Moxibustion are higher than that of high-intensity training group,CD3+,CD4+ are of significance(P＜0.01,P＜0.05).
Comparing to the control group,the spleen index,thymus index,the proliferation ability of lymphocytes of the high-intensity training group were significantly reduced(P＜0.01, P＜0.01,P＜0.05),and the three indexes of the group of the high-intensity training group with Moxibustion and electro-Acupuncture and the group of high-intensity training with Moxibustion are on a downward trend though,but no statistical significance(P＞0.05),in the numerical,the spleen index and thymus index of the two groups with Acupuncture are significantly higher than that of high-intensity training group(P＜0.01).
Comparing to the control group,the CRH,ACTH,CORT of the high-intensity training group increased all,in which ACTH,CORT are significant(P＜0.01,P＜0.05),and the CRH,CORT of the group of high-intensity training with Moxibustion has been a downward trend,but no significant(P＞0.05),despite the ACTH of the group of high-intensity training with Moxibustion significantly higher than that of the control group (P＜0.01),but comparing to the group of high-intensity training the CRH,ACTH,CORT reduced all,in which ACTH,CORT is very significant(P＜0.01) statistically;Compared to the control group,CRH,ACTH of the group of high-intensity training with electro-Acupuncture and Moxibustion slightly increased,and CORT is downward trend, but they are not significant(P＞0.05),compared to the group of high-intensity training group,the CRH,ACTH and CORT of the group of high-intensity training with electro-Acupuncture and Moxibustion reduce all,in which ACTH,CORT are significant (P＜0.01) statistically.Comparing each other amongst Acupuncture groups,the CRH, CORT of the group of high-intensity training with electro-Acupuncture and Moxibustion are slightly higher than that of the group of high-intensity training with moxibustion,but it is not significant statistically(P＞0.05),the ACTH of the group of high-intensity training with Moxibustion and electro-Acupuncture are significantly lower than that of the group of high-intensity training with moxibustion(P＜0.05).
1.After high-intensity swimming stress of eight weeks,mind,appearance,body weight of rats are deteriorated,the rats' blood immune indexes:IL-2,γ-IFN,NKC,CD3~+、CD4~+、ratio of CD4~+/CD8~+ of T-lymphocyte subsets,and the spleen index,thymus index,blood lymphocytes to mitogen in vitro proliferation of PHA showed a significant decrease,while the immunosuppressive factor SIL-2R concentration,HPA axis in CRH,ACTH,CORT were significantly increased.
2.Simply putting moxibustionon Baihui,Guanyuan,Zusanli not only can improve the percentage of CD3~+,and can significantly increase the percentage of CD4~+ of T helper cells,reduced concentration of CD8~+ of suppressive T cells,caused the ratio of CD4~+/CD8~+ increased,to some extent can prevent the decline of proliferative response of thymus index,spleen index and blood lymphocytes to PHA mitogen under the state of long-term high-intensity exercise stress,but decline of NK cells' quantity lack of positive regulatory role under the state of long-term high-intensity exercise stress.Moxibustionplay limited regulatory role on IL-2-IFN-NKC immunoregulatory network,it can still prevent to some extent the decline of IL-2.
3.Electro-Acupuncture on the points of Bilateral Zusanli and Moxibustion on the points of Guanyuan and Baihui can not only effectively reduced the decrease in NK cells,increased the ratio of CD4~+/CD8~+,but also promote CD3~+、CD4~+ with a significant role,can also effectively prevent the decline vitro proliferative response of thymus index,spleen index and blood lymphocytes to PHA mitogen.o rats under the state of long-term high-intensity exercise stress.They can play a more comprehensive positive role on adjusting IL-2-IFN-NKC immunoregulatory network than simply Moxibustion under the state of long-term high-intensity exercise stress,which can effectively prevent decline in the number of IL-2,γ-IFN and NK cells of the rats.
4 Whether simple Moxibustion or electro-Acupuncture combined with Moxibustioncan regulate the hormone of the HPA axis from the three levels,improve the function of HPA axis to avoid HPA axis function sustained over-hyper,through reducing ACTH,CORT and CRH content.It can achieve a benign intervention role disorders of the neuroendocrinef induced by long-term strenuous exercise stress.
Conclusions:
1 Long-term high-intensity exercise stress can not only lead to the nerve appearance, body weight of rats deteriorated,and can cause shrinkage of the structure of immune organs of rats,the functional degradation,reduction in the number of immune cells,inhibit secretion of immune molecules,IL-2-IFN-NKC immunoregulatory network disorder,show that exercise induced rats immunosuppression.And long-term high-intensity exercise stress cause the continued excessive HPA axis hyperfunction,resulting in excessive synthesis of CORT rats,and then resulting in neuroendocrine-immune dysfunction,which may be the main reasons of leading to emergence of immune function suppression(exercise-induced immune suppression)
2 Acupuncture can reduce the long-term strenuous exercise stress on the immune function of the damage,and can effectively prevent exercise-induced immunosuppression,its mechanism may be:on the one hand,correcting dysfunction of the HPA axis,and then down the concentration of CORT to achieve the protective effect of immune function of the body of long-term high-intensity exercise stress state,and effectively prevent the movement of the occurrence of immunosuppression;on the other hand,related to directly adjustment disorder of IL-2-IFN-NKC immunoregulatory network and then positive regulation of closely linked immunodeficiency.
3 the regulation that Acupuncture pay on HPA axis function disorder of rat of long-term high-intensity exercise stress of rats is multi-channel and multi-level,different methods of Acupuncture intervention play a positive role differently,the results of this study show: Acupuncture combined with Moxibustion pay more comprehensive adjustment on the immune function of rats than the simple Moxibustion.
引文
[1]颜军,尹剑春,孙宏,等.强迫游泳和冷刺激对大鼠白细胞介素2和β-内腓肽的影响[J].中国心理卫生杂志,2002,16(12):811-813.
[2]邵枫,林文娟,王玮雯,等.电击信号应激对大鼠体液免疫及内分泌功能的影响[J].心理学报,2000,32(4):428-432.
[3]Nieman DC.Is infection risk linked to exercise workload[J].Med Sci Sports Exerc 2000,(Suppl):S406-411.
[4]Nieman DC,Johanseen LM,Lee JW,et al.Infections episodes in runners before an after the Los Angeles Marathon[J].J Sports Med Phys Fitness,1990,(30):216-218.
[5]Mackinnon LT,Hooper SL.Plasma Glutamine concentration and upper respiratory tract infection during overtraining in elite swimmers[J].Med Sci Sports Exerc 1996,28:285-290.
[6]Mackinnon LT,Ginn EM,Seymour GJ.Temporal relationship between exercise-induced decreases in salivary IgA and subsepuent appearance of upper respiratory tract infection in elite athletes[J].Aust J Sci Med Sport 1993,25:94-99.
[7]Heath GW,Ford ES,Craven TE,et al.Exercise and the incidence of upper respiratory tract infections[J].Med Sci Sports Exerc 1991,23:152-157.
[8]Hong S,Farag NH,Nelesen RA,et al.Effects of regular exercise on lymphocyte subsets and CD621L after psychological vs.physical stress[J].J Psychosom Res 2004;56(3):363-70.
[9]Pema FM,Schneiderman N,LaPerriere A.Psychological stress,exercise and immunity[J].Int J Sports Med 1997;18 Suppl 1:S78-83.
[10]Deuster PA,Zelazowska EB,Singh A,et al.Expression of lymphocyte subsets after exercise and dexamethasone in high and low stress responders[J]..Med Sci Sports Exerc 1999;31(12):1799-806.
[11]Quadrilatero J,Boudreau J,Hoffman-Goetz L.Lymphocyte distribution in mouse submandibular lymph nodes in response to acute treadmill exercise[J]..Can J Physiol Pharmacol 2003;81(10):927.
[12]Mackinnon LT.Immunity in atheles[J]..Int J Sports Med 1997;18 Suppl 1;S62-8.
[13]Woods J,Lu Q,Ceddia MA,et al.Special feature for the Olympics:effects of exercise on the immune system:exercise-induced modulation of macrophage function[J]..Immunol Cell Biol 2000;7895):545-53.
[14]pedersen BK,UIIum H.NK cell response to physical activity:possible mechanisms of acting[J].Med Sci Sports Exerc 1994;26(2):140-146.
[15]Nkataman JY,Pendergast LR Effect of dietary intake on immune function in athletes[J].Sports Medicine,2002;3295):32-37.
[16]leesonM BishopNC Special feature for the Olynpics:effects of exercise on the immune system modification of immune responses to exercise by carbohydrate glutamineandanti-oxidant supplements[J].immunologyand cellbiology,2000;78(5):54-61.
[17]Mackinnon LT.Chronic exercise training effects on immune function[J].Medicine and science in sports and exercise 2000 Jul;32(7suppl),pp S369-76.
[18]Peterson EK,Hoffman—Goetz L,Exercise and the immune system regulation integration and adaptation[J].physiological reviews 2000 Jul;80,pp1055-81.
[19]Glessor M,Bishopl C:Eliteathlete immunology:importance of nutrition international journal of sports medicine 2000 May[J].;21 suppl 1,99 S 44-50.
[20]郝选明 运动员免疫功能降低的机理及其调理措施[J].上海体育科研2003:24(4):47-49.
[21]金花,莫雷.应激对免疫功能的影响及其调控手段[J]..中国运动医学杂志.2004;23(1):106-110.
[22]桂华丽,付克翠.运动对机体免疫机能的影响及其调节作用[J]..安徽体育科技.2003;24(1):87-89.
[23]刘晓丹,吴卫兵.运动与免疫细胞及调节机理综述[J]..河北体育学院学报2004;18(1):6-8.
[24]Henson DA,et al.Carbohydrate supple mention and the lymphocyte proliferative response to long endutance running[J]..Int J sports Med 1998;19:574-580.
[25]钱威.运动中应激激素的反应及对免疫功能的影响[J].广州体育学院学报.2001;21(1):70-73.
[26]鞠海兵,沈菲菲,舒子正.运动应激与下丘脑-垂体-肾上腺轴[J]..国外医学生理病理科学与临床分册.2003,22(5):514.
[27]范少光,丁桂风.神经内分泌系统与免疫系统之间的关系[J]..中国应用生理学杂志.1992;8(3):281-288.
[28]饶艳.运动和神经内分泌对巨噬细胞功能的影响[J]..吉林体育学院学报,2002,18(4):58-61.
[29]李晓泓,韩毳.艾灸“大椎”穴抗应激作用的实验研究[J].中国行为医学科学.2002,11(5).-495-497.
[30]杜莅娜,姜建伟.纳洛酮和电针改善创伤应激大鼠的免疫功能[J].生理学报.1998,50(6).636-642.
[31]程晓东,吴根诚,姜建伟,等.持续电针刺激调节创伤大鼠脾淋巴细胞体外增殖反应的动态观察[J].中国免疫学杂志,1997,13(2):68.
[32]李红,朱梅菊,高顺生.针灸足三里穴对小鼠运动能力及部分免疫指标的影响[J]..中国运动医学杂志.2004,23.
[33]杨翼,李章华.针灸消除运动性疲劳的研究进展与思考[J]..中国运动医学杂志.2002,21(5).-499-501.
[34]张允兰,李延斌,齐登伟.中医学中的免疫学思想与现代免疫[J].中医函授通讯,1998,17 (3):37.
[35]向楠.正气与体液免疫相关性研究[J].河北中医杂志,1997,19(1):55.
[36]朱文莲,刘仁权.艾灸大椎穴对免疫低下小鼠巨噬细胞吞噬功能的影响[J].北京中医药大学学报,2005,28(1):89-90.
[37]吕琳.针灸调节肿瘤免疫的临床及实验研究进展[J].辽宁中医杂志,2001,28(10):638-640.
[38]唐照亮,宋小鸽,侯正明,等.灸疗抗炎免疫作用的实验研究[J].中国针灸,1997,(4):233-235.
[39]严卫星,郑震林,王建鹤,等.β-内腓肽对细胞免疫功能的调节[J].南通医学院血报,1994,14(2):132.
[40]翟道荡,陈汉平,王瑞珍,等.直接灸免疫调节作用的β-内腓肽机制[J].上海针灸杂志,1994,13(5):223.
[41]高巍,黄裕新,陈洪,等.脑肠肽在针刺调节免疫反应中的作用[J].中国免疫学杂志,2001,17(9):473.
[42]高巍,黄裕新,陈洪,等.电针“足三里”对大鼠脑肠肽含量的影响及其对免疫系统的调控作用[J].针刺研究,2000,27(1):50
[43]赵建础,刘文琴,赵续民,等.新生期小鼠辣椒素处理对针剌调节免疫反应的影响[J].针刺研究,1996,21(3):36.
[44]朱笛霓,赵建础,王力菲,等.免疫对下丘脑腹内侧核神经元放电率的变化及电针、弓状核、蓝斑对其调节作用[J].针刺研究,1996,21(3):32
[45]刘文琴,赵续民,赵建础,等.肾上腺皮质系统在电针调节免疫反应中的作用[J].针刺研究,1996,21(2):71.
[46]赵续民,刘文琴,赵建础,等.纳洛酮对电针调节整体免疫反应的影响[J].针刺研究,1995,20(4):39.
[47]赵续民,刘文琴,赵建础,等.外周应用密胆碱对针刺调节免疫反应的影响[J].针刺研究,1995,20(2):59.
[48]吴滨,周荣兴,周鸣生,等.针灸治疗对恶性肿瘤患者细胞免疫调节的影响[J].针刺研究,1995,20(3):67.
[49]王芗斌,吴炳煌.论针灸与机体免疫功能的关系[J].现代康复,2001,5(7):124.
[50]金其贯,吴凤起.运动性免疫抑制的机制研究[J].西安体育学院学报,2004,21(5):48-52.
[51]李丽,常泼,夏冬.运动性免疫抑制[J].沈阳体育学院学报,2004,23(2):294-296.
[52]陈碧云.中药制剂对大鼠血清激素水平和运动能力的影响[J].中国运动医学杂志,1996,15(2):102.
[53]曹建明.补脾活血中药对小鼠免疫功能的影响[J]..体育科学,1996,16(2):51:53
[54]焦颖.“益寿回春汤”对竞技运动员机能状态及运动能力影响的综合观察[J]..中国运动医学杂志,1992,11(1):36
[55]熊若虹,苏全生,袁琼嘉.补充复力康合剂对训练大鼠运动能力及血清T、C、GH水平的影响[J].中国运动医学杂志,2004,23(3)277-279.
[56]乔玉成,张红娟,卢向阳,等.补充五子壮阳汤对大运动量训练大鼠雄激素水平及运动能力的影响[J].中国运动医学杂志,2004,23(3):307-308.
[57]施建蓉,庄创青,郭瑞新.复方红景天对大强度训练大鼠免疫功能的调节作用[J].中国免疫学杂志,1999,15(4):171.
[58]于洋.健脾理气中药对运动员细胞免疫功能的研究[J]..沈阳体育学院学报,1993,2:19-20.
[59]郭建军,杨则宜,谢敏毫,等.中药复方“体复康”对剧烈运动后大鼠巨噬细胞功能恢复的影响[J].中国运动医学杂志,1999,18(3):231-232.
[60]曹光民.“第二代天成金芝营养液”对运动员生理功能的影响[J]..中国运动医学杂志,1998,3:270-273.
[61]周志勇.地球王口服液对运动员免疫功能影响的研究[J]..上海体育学院学报,1995(2):46-48.
[62]杨维益.运动性疲劳与中医肝脏的关系[J]..北京中医药大学生学报,1996,1:17-18.
[63]陈宗旭.神经—内分泌—免疫网络研究概况及其与中医肝脏关系的探讨[J]..北京中医药大学学报,1995,4:7-10.
[64]汪军,姜建伟,蔡三军,等.电针对消化道肿瘤患者围手术及围化疗期T淋巴细胞亚群和淋巴细胞转化功能的影响[J].2004,23(11):5-8.
[65]袁红,袁丽荣,马惠芳,等.针刺对应激状态下小鼠细胞免疫功能的影响的实验研究[J].针刺研究,2002,27(3):211-222.
[66]吴滨,周荣兴,周鸣生,等.针刺治疗对恶性肿瘤患者细胞免疫调节的影响[J]..针刺研究,1995,20(3):67.
[67]赵蓉,马春红,谭连红,等.针刺对免疫抑制大鼠巨噬细胞功能的影响.针刺研究,1994,19(2):66-67.
[68]朱梅,高洪泉,刘瑞丰,等.针刺足三里、关元穴区对老年大鼠肝脏内巨噬细胞功能影响的实验研究[J]..针灸临床杂志,2003,19(6):52.
[69]杨会芹,张联科.针灸对红细胞免疫功能调节的研究[J].陕西中医学院学报,2004,27(5):76-77.
[70]何学斌,吴耀,罗济民,等.针灸对气虚大鼠红细胞免疫功能影响的实验研究[J].针刺研究,2003,28(3):189-191.
[71]张英,刘又香,孙国杰.不同灸治时辰对阳虚小鼠免疫功能影响的比较[J].湖北中医杂志,1998,20(2):53-54.
[72]严桂珍,郑家铿,许少锋,等.针刺“三阴交”穴择时治疗对脾阳虚家兔免疫功能的影响[J].中国针灸,2001,21(12):735-737.
[73]马振亚,王益民,范青生.针刺对正常动物IL-2-IFN-NKC免疫调节网络的影响[J].西安医 科大学学报,1991,12(2):116.
[74]胡丽娜,陈汉平,李文.针刺对人体免疫机能影响的腧穴特异性研究[J]..江苏中医,1990,2:20.
[75]陈雄华.针灸抗肿瘤免疫效应的临床与实验研究概况[J].湖北中医杂志,1998,20(5):59-60.
[76]王强.针灸对类风湿性关节炎患者免疫功能及抗自由基酶类的影响[J].中国中医药信息杂志,2001,8(8):68.
[77]崔道荡,李鼎,王瑞玲,等.艾灸关元穴抗小鼠移植性肝癌(HAC)的神经免疫学机理研究[J].针灸学报,1991,(3):10.
[78]裴建,陈汉平,赵粹英,等.艾灸对荷瘤小鼠免疫功能的增强作用[J].上海免疫学杂志1997,17(5):297.
[79]吴松,陈泽斌,王华.针灸调节免疫功能的研究进展[J]..湖北中医杂志,2005,27(12):48-49.
[80]陈雄华,刘又香,王华,等.针灸“足三里”、“关元”穴对阳虚大鼠免疫功能影响的比较研究[J].中国针灸,2000,20(9):555-557.
[81]程晓东,姜建伟,吴根诚,等.针刺对手术创伤大鼠T细胞活化早期酪氨酸蛋白激酶活性的影响[J].针刺研究,2002,27(3):211.
[82]陈雪琼,吕璇,陈影红.温针灸对运动员大强度负荷后疲劳恢复的初探[J1.现代康复,2000,4(11):1722.
[83]王文远,朱栋,王辉,等.平衡针刺治疗运动性疲劳症的临床研究[J].中国针灸,1999,(1):13-15.
[84]向志勇,陈元武,郁时兵,等.针灸对运动性疲劳影响的实验观察[J].山东体育科技,1998,20(3):25-27.
[85]应晓明,方剑乔.针灸治疗运动性疲劳的研究进展[J]..针灸临床杂志,2006,22(3):53-54.
[86]王家祥,王金勇.穴位艾灸对男性运动员血清睾酮的影响[J].南京体育学院学报(自然科学版),2005,4(1):29-40.
[87]浦钧宗,唐培.不同训练量对动物免疫指标影响的研究[J].中国运动医学杂志,1996,15(3):39-41.
[88]潘华山.针灸三阴交穴对小白鼠运动能力与某些免疫指标的影响[J],广州体育学院学报,2005,25(4):34-36.
[89]潘华山.针刺三阴交穴对女性运动员运动能力与某些免疫指标的影响[J].山西师大体育学院学报,2007,22(1):126-127.
[90]林飞卿.主编.医学基础免疫学[M].上海医科大学出版社,1990:72-78.
[91]王瑞元.主编.运动生理学[M].北京:人民体育卫生出版社,2002,9:469-472.
[92]金其贯,冯美云.谷氨酰胺和精氨酸对运动性免疫抑制干预作用的研究[J]..北京体育大学学报,2005,28(8):1075-1077.
[93]马东晓,惠燕,姜丽.维生素与运动免疫功能[J]..吉林体育学院学报,2001,17(4):50-51.
[94]姚俊,李卫平,赖晓红.硒和运动[J]..冰雪运动,2002(3):71-73.
[95]Fawzy FI,Fawzy NW,Hyun CS,et al.Malignant melanoma:effect of an early structured psychiatric intervention,coping and affective state on recurrence and survival six years latter[J]..Psychatic,1993,50:681-689.
[96]阎克乐,张月娟,张文彩,等.心理神经免疫学[M].心理科学,1997,20(4):357-358.
[97]陈云飞,杨文佳,符胜光,等.电针对慢性疲劳大鼠下丘脑—垂体—肾上腺轴激素水平的影响[J].中华中医药学刊,2007(6):1146-1148.
[98]郭建军,杨则宜,杨志勇,等.不同训练强度对大鼠腹腔巨噬细胞分泌免疫细胞因子影响的实验研究[J].中国运动医学杂志,1999,18(2):121-122.
[99]余传霖,叶天星,陆德源,等.现代医学免疫学[M].上海:上海医科大学出版社,1998:132-133.
[100]马振亚,王益民,范青生.针刺对肾虚动物IL-2-IFN-NKC免疫调节网络的影响[J].针刺研究,1992,17(2):139.
[101]杨宁波,吴爱勤,陈文,等.慢性心理应激小鼠胸腺免疫细胞凋亡的研究[J].中国行为医学科学,2006,15(11):986-988.
[102]林文娟,王玮雯.慢性情绪应激对大鼠行为、神经内分泌和免疫反应的影响:一个新的情绪应激模型[J].科学通报,2003,48(9):926-929.
[2]邵枫,林文娟,王玮雯,等.电击信号应激对大鼠体液免疫及内分泌功能的影响[J].心理学报,2000,32(4):428-432.
[3]Nieman DC.Is infection risk linked to exercise workload[J].Med Sci Sports Exerc 2000,(Suppl):S406-411.
[4]Nieman DC,Johanseen LM,Lee JW,et al.Infections episodes in runners before an after the Los Angeles Marathon[J].J Sports Med Phys Fitness,1990,(30):216-218.
[5]Mackinnon LT,Hooper SL.Plasma Glutamine concentration and upper respiratory tract infection during overtraining in elite swimmers[J].Med Sci Sports Exerc 1996,28:285-290.
[6]Mackinnon LT,Ginn EM,Seymour GJ.Temporal relationship between exercise-induced decreases in salivary IgA and subsepuent appearance of upper respiratory tract infection in elite athletes[J].Aust J Sci Med Sport 1993,25:94-99.
[7]Heath GW,Ford ES,Craven TE,et al.Exercise and the incidence of upper respiratory tract infections[J].Med Sci Sports Exerc 1991,23:152-157.
[8]Hong S,Farag NH,Nelesen RA,et al.Effects of regular exercise on lymphocyte subsets and CD621L after psychological vs.physical stress[J].J Psychosom Res 2004;56(3):363-70.
[9]Pema FM,Schneiderman N,LaPerriere A.Psychological stress,exercise and immunity[J].Int J Sports Med 1997;18 Suppl 1:S78-83.
[10]Deuster PA,Zelazowska EB,Singh A,et al.Expression of lymphocyte subsets after exercise and dexamethasone in high and low stress responders[J]..Med Sci Sports Exerc 1999;31(12):1799-806.
[11]Quadrilatero J,Boudreau J,Hoffman-Goetz L.Lymphocyte distribution in mouse submandibular lymph nodes in response to acute treadmill exercise[J]..Can J Physiol Pharmacol 2003;81(10):927.
[12]Mackinnon LT.Immunity in atheles[J]..Int J Sports Med 1997;18 Suppl 1;S62-8.
[13]Woods J,Lu Q,Ceddia MA,et al.Special feature for the Olympics:effects of exercise on the immune system:exercise-induced modulation of macrophage function[J]..Immunol Cell Biol 2000;7895):545-53.
[14]pedersen BK,UIIum H.NK cell response to physical activity:possible mechanisms of acting[J].Med Sci Sports Exerc 1994;26(2):140-146.
[15]Nkataman JY,Pendergast LR Effect of dietary intake on immune function in athletes[J].Sports Medicine,2002;3295):32-37.
[16]leesonM BishopNC Special feature for the Olynpics:effects of exercise on the immune system modification of immune responses to exercise by carbohydrate glutamineandanti-oxidant supplements[J].immunologyand cellbiology,2000;78(5):54-61.
[17]Mackinnon LT.Chronic exercise training effects on immune function[J].Medicine and science in sports and exercise 2000 Jul;32(7suppl),pp S369-76.
[18]Peterson EK,Hoffman—Goetz L,Exercise and the immune system regulation integration and adaptation[J].physiological reviews 2000 Jul;80,pp1055-81.
[19]Glessor M,Bishopl C:Eliteathlete immunology:importance of nutrition international journal of sports medicine 2000 May[J].;21 suppl 1,99 S 44-50.
[20]郝选明 运动员免疫功能降低的机理及其调理措施[J].上海体育科研2003:24(4):47-49.
[21]金花,莫雷.应激对免疫功能的影响及其调控手段[J]..中国运动医学杂志.2004;23(1):106-110.
[22]桂华丽,付克翠.运动对机体免疫机能的影响及其调节作用[J]..安徽体育科技.2003;24(1):87-89.
[23]刘晓丹,吴卫兵.运动与免疫细胞及调节机理综述[J]..河北体育学院学报2004;18(1):6-8.
[24]Henson DA,et al.Carbohydrate supple mention and the lymphocyte proliferative response to long endutance running[J]..Int J sports Med 1998;19:574-580.
[25]钱威.运动中应激激素的反应及对免疫功能的影响[J].广州体育学院学报.2001;21(1):70-73.
[26]鞠海兵,沈菲菲,舒子正.运动应激与下丘脑-垂体-肾上腺轴[J]..国外医学生理病理科学与临床分册.2003,22(5):514.
[27]范少光,丁桂风.神经内分泌系统与免疫系统之间的关系[J]..中国应用生理学杂志.1992;8(3):281-288.
[28]饶艳.运动和神经内分泌对巨噬细胞功能的影响[J]..吉林体育学院学报,2002,18(4):58-61.
[29]李晓泓,韩毳.艾灸“大椎”穴抗应激作用的实验研究[J].中国行为医学科学.2002,11(5).-495-497.
[30]杜莅娜,姜建伟.纳洛酮和电针改善创伤应激大鼠的免疫功能[J].生理学报.1998,50(6).636-642.
[31]程晓东,吴根诚,姜建伟,等.持续电针刺激调节创伤大鼠脾淋巴细胞体外增殖反应的动态观察[J].中国免疫学杂志,1997,13(2):68.
[32]李红,朱梅菊,高顺生.针灸足三里穴对小鼠运动能力及部分免疫指标的影响[J]..中国运动医学杂志.2004,23.
[33]杨翼,李章华.针灸消除运动性疲劳的研究进展与思考[J]..中国运动医学杂志.2002,21(5).-499-501.
[34]张允兰,李延斌,齐登伟.中医学中的免疫学思想与现代免疫[J].中医函授通讯,1998,17 (3):37.
[35]向楠.正气与体液免疫相关性研究[J].河北中医杂志,1997,19(1):55.
[36]朱文莲,刘仁权.艾灸大椎穴对免疫低下小鼠巨噬细胞吞噬功能的影响[J].北京中医药大学学报,2005,28(1):89-90.
[37]吕琳.针灸调节肿瘤免疫的临床及实验研究进展[J].辽宁中医杂志,2001,28(10):638-640.
[38]唐照亮,宋小鸽,侯正明,等.灸疗抗炎免疫作用的实验研究[J].中国针灸,1997,(4):233-235.
[39]严卫星,郑震林,王建鹤,等.β-内腓肽对细胞免疫功能的调节[J].南通医学院血报,1994,14(2):132.
[40]翟道荡,陈汉平,王瑞珍,等.直接灸免疫调节作用的β-内腓肽机制[J].上海针灸杂志,1994,13(5):223.
[41]高巍,黄裕新,陈洪,等.脑肠肽在针刺调节免疫反应中的作用[J].中国免疫学杂志,2001,17(9):473.
[42]高巍,黄裕新,陈洪,等.电针“足三里”对大鼠脑肠肽含量的影响及其对免疫系统的调控作用[J].针刺研究,2000,27(1):50
[43]赵建础,刘文琴,赵续民,等.新生期小鼠辣椒素处理对针剌调节免疫反应的影响[J].针刺研究,1996,21(3):36.
[44]朱笛霓,赵建础,王力菲,等.免疫对下丘脑腹内侧核神经元放电率的变化及电针、弓状核、蓝斑对其调节作用[J].针刺研究,1996,21(3):32
[45]刘文琴,赵续民,赵建础,等.肾上腺皮质系统在电针调节免疫反应中的作用[J].针刺研究,1996,21(2):71.
[46]赵续民,刘文琴,赵建础,等.纳洛酮对电针调节整体免疫反应的影响[J].针刺研究,1995,20(4):39.
[47]赵续民,刘文琴,赵建础,等.外周应用密胆碱对针刺调节免疫反应的影响[J].针刺研究,1995,20(2):59.
[48]吴滨,周荣兴,周鸣生,等.针灸治疗对恶性肿瘤患者细胞免疫调节的影响[J].针刺研究,1995,20(3):67.
[49]王芗斌,吴炳煌.论针灸与机体免疫功能的关系[J].现代康复,2001,5(7):124.
[50]金其贯,吴凤起.运动性免疫抑制的机制研究[J].西安体育学院学报,2004,21(5):48-52.
[51]李丽,常泼,夏冬.运动性免疫抑制[J].沈阳体育学院学报,2004,23(2):294-296.
[52]陈碧云.中药制剂对大鼠血清激素水平和运动能力的影响[J].中国运动医学杂志,1996,15(2):102.
[53]曹建明.补脾活血中药对小鼠免疫功能的影响[J]..体育科学,1996,16(2):51:53
[54]焦颖.“益寿回春汤”对竞技运动员机能状态及运动能力影响的综合观察[J]..中国运动医学杂志,1992,11(1):36
[55]熊若虹,苏全生,袁琼嘉.补充复力康合剂对训练大鼠运动能力及血清T、C、GH水平的影响[J].中国运动医学杂志,2004,23(3)277-279.
[56]乔玉成,张红娟,卢向阳,等.补充五子壮阳汤对大运动量训练大鼠雄激素水平及运动能力的影响[J].中国运动医学杂志,2004,23(3):307-308.
[57]施建蓉,庄创青,郭瑞新.复方红景天对大强度训练大鼠免疫功能的调节作用[J].中国免疫学杂志,1999,15(4):171.
[58]于洋.健脾理气中药对运动员细胞免疫功能的研究[J]..沈阳体育学院学报,1993,2:19-20.
[59]郭建军,杨则宜,谢敏毫,等.中药复方“体复康”对剧烈运动后大鼠巨噬细胞功能恢复的影响[J].中国运动医学杂志,1999,18(3):231-232.
[60]曹光民.“第二代天成金芝营养液”对运动员生理功能的影响[J]..中国运动医学杂志,1998,3:270-273.
[61]周志勇.地球王口服液对运动员免疫功能影响的研究[J]..上海体育学院学报,1995(2):46-48.
[62]杨维益.运动性疲劳与中医肝脏的关系[J]..北京中医药大学生学报,1996,1:17-18.
[63]陈宗旭.神经—内分泌—免疫网络研究概况及其与中医肝脏关系的探讨[J]..北京中医药大学学报,1995,4:7-10.
[64]汪军,姜建伟,蔡三军,等.电针对消化道肿瘤患者围手术及围化疗期T淋巴细胞亚群和淋巴细胞转化功能的影响[J].2004,23(11):5-8.
[65]袁红,袁丽荣,马惠芳,等.针刺对应激状态下小鼠细胞免疫功能的影响的实验研究[J].针刺研究,2002,27(3):211-222.
[66]吴滨,周荣兴,周鸣生,等.针刺治疗对恶性肿瘤患者细胞免疫调节的影响[J]..针刺研究,1995,20(3):67.
[67]赵蓉,马春红,谭连红,等.针刺对免疫抑制大鼠巨噬细胞功能的影响.针刺研究,1994,19(2):66-67.
[68]朱梅,高洪泉,刘瑞丰,等.针刺足三里、关元穴区对老年大鼠肝脏内巨噬细胞功能影响的实验研究[J]..针灸临床杂志,2003,19(6):52.
[69]杨会芹,张联科.针灸对红细胞免疫功能调节的研究[J].陕西中医学院学报,2004,27(5):76-77.
[70]何学斌,吴耀,罗济民,等.针灸对气虚大鼠红细胞免疫功能影响的实验研究[J].针刺研究,2003,28(3):189-191.
[71]张英,刘又香,孙国杰.不同灸治时辰对阳虚小鼠免疫功能影响的比较[J].湖北中医杂志,1998,20(2):53-54.
[72]严桂珍,郑家铿,许少锋,等.针刺“三阴交”穴择时治疗对脾阳虚家兔免疫功能的影响[J].中国针灸,2001,21(12):735-737.
[73]马振亚,王益民,范青生.针刺对正常动物IL-2-IFN-NKC免疫调节网络的影响[J].西安医 科大学学报,1991,12(2):116.
[74]胡丽娜,陈汉平,李文.针刺对人体免疫机能影响的腧穴特异性研究[J]..江苏中医,1990,2:20.
[75]陈雄华.针灸抗肿瘤免疫效应的临床与实验研究概况[J].湖北中医杂志,1998,20(5):59-60.
[76]王强.针灸对类风湿性关节炎患者免疫功能及抗自由基酶类的影响[J].中国中医药信息杂志,2001,8(8):68.
[77]崔道荡,李鼎,王瑞玲,等.艾灸关元穴抗小鼠移植性肝癌(HAC)的神经免疫学机理研究[J].针灸学报,1991,(3):10.
[78]裴建,陈汉平,赵粹英,等.艾灸对荷瘤小鼠免疫功能的增强作用[J].上海免疫学杂志1997,17(5):297.
[79]吴松,陈泽斌,王华.针灸调节免疫功能的研究进展[J]..湖北中医杂志,2005,27(12):48-49.
[80]陈雄华,刘又香,王华,等.针灸“足三里”、“关元”穴对阳虚大鼠免疫功能影响的比较研究[J].中国针灸,2000,20(9):555-557.
[81]程晓东,姜建伟,吴根诚,等.针刺对手术创伤大鼠T细胞活化早期酪氨酸蛋白激酶活性的影响[J].针刺研究,2002,27(3):211.
[82]陈雪琼,吕璇,陈影红.温针灸对运动员大强度负荷后疲劳恢复的初探[J1.现代康复,2000,4(11):1722.
[83]王文远,朱栋,王辉,等.平衡针刺治疗运动性疲劳症的临床研究[J].中国针灸,1999,(1):13-15.
[84]向志勇,陈元武,郁时兵,等.针灸对运动性疲劳影响的实验观察[J].山东体育科技,1998,20(3):25-27.
[85]应晓明,方剑乔.针灸治疗运动性疲劳的研究进展[J]..针灸临床杂志,2006,22(3):53-54.
[86]王家祥,王金勇.穴位艾灸对男性运动员血清睾酮的影响[J].南京体育学院学报(自然科学版),2005,4(1):29-40.
[87]浦钧宗,唐培.不同训练量对动物免疫指标影响的研究[J].中国运动医学杂志,1996,15(3):39-41.
[88]潘华山.针灸三阴交穴对小白鼠运动能力与某些免疫指标的影响[J],广州体育学院学报,2005,25(4):34-36.
[89]潘华山.针刺三阴交穴对女性运动员运动能力与某些免疫指标的影响[J].山西师大体育学院学报,2007,22(1):126-127.
[90]林飞卿.主编.医学基础免疫学[M].上海医科大学出版社,1990:72-78.
[91]王瑞元.主编.运动生理学[M].北京:人民体育卫生出版社,2002,9:469-472.
[92]金其贯,冯美云.谷氨酰胺和精氨酸对运动性免疫抑制干预作用的研究[J]..北京体育大学学报,2005,28(8):1075-1077.
[93]马东晓,惠燕,姜丽.维生素与运动免疫功能[J]..吉林体育学院学报,2001,17(4):50-51.
[94]姚俊,李卫平,赖晓红.硒和运动[J]..冰雪运动,2002(3):71-73.
[95]Fawzy FI,Fawzy NW,Hyun CS,et al.Malignant melanoma:effect of an early structured psychiatric intervention,coping and affective state on recurrence and survival six years latter[J]..Psychatic,1993,50:681-689.
[96]阎克乐,张月娟,张文彩,等.心理神经免疫学[M].心理科学,1997,20(4):357-358.
[97]陈云飞,杨文佳,符胜光,等.电针对慢性疲劳大鼠下丘脑—垂体—肾上腺轴激素水平的影响[J].中华中医药学刊,2007(6):1146-1148.
[98]郭建军,杨则宜,杨志勇,等.不同训练强度对大鼠腹腔巨噬细胞分泌免疫细胞因子影响的实验研究[J].中国运动医学杂志,1999,18(2):121-122.
[99]余传霖,叶天星,陆德源,等.现代医学免疫学[M].上海:上海医科大学出版社,1998:132-133.
[100]马振亚,王益民,范青生.针刺对肾虚动物IL-2-IFN-NKC免疫调节网络的影响[J].针刺研究,1992,17(2):139.
[101]杨宁波,吴爱勤,陈文,等.慢性心理应激小鼠胸腺免疫细胞凋亡的研究[J].中国行为医学科学,2006,15(11):986-988.
[102]林文娟,王玮雯.慢性情绪应激对大鼠行为、神经内分泌和免疫反应的影响:一个新的情绪应激模型[J].科学通报,2003,48(9):926-929.