多壁碳纳米管的生物相容性研究
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摘要
纳米技术是20世纪80年代末、90年代初才逐步发展起来的前沿、交叉性新型学科领域,它的迅猛发展将在21世纪促使激活所有工业领域产生一场革命性的变化。自从发现碳纳米管(CNTs)以来,CNTs就以它良好的导电、机械及半导体性能而成为材料科学领域的一颗新星,有着广泛的应用和商业价值。有关研究认为CNTs生物相容性较好,故其在生物医用材料中有极大的应用价值。
目前以生物医学应用为目标的相关研究主要涉及对碳纳米管进行表面修饰,使其具有阻止蛋白质非特异性吸附和识别特定蛋白分子的功能；利用碳纳米管的特殊形状和电学性质,研究细胞的体外生长等。本论文研究了碳纸基多壁碳纳米管(MWCNTs)表面的溶血、血小板粘附和血液蛋白质吸附情况以及细胞在该材料上的黏附和生长行为。结果表明：掺氮和不掺氮多壁碳纳米管材料为细胞提供了接近天然细胞外基质的人造环境,具有显著促进细胞粘附和长时间增殖的功能,具有良好的细胞相容性。尽管多壁碳纳米管和碳纸两种材料表面吸附的白蛋白(Albumin, Alb)数量一样,碳纸吸附的纤维蛋白原(Fibrinogen, Fib)和免疫球蛋白(Immunoglobulin, IgG)要少于多壁碳纳米管,但多壁碳纳米管的溶血率和血小板粘附率都要低于碳纸,这意味着相对碳纸而言疏水性更好的多壁碳纳米管可能抑制血小板的粘附和血栓的形成能力更强于碳纸,具有一定的血液相容性。
研究结果表明,这种多壁碳纳米管具备生物材料在物理化学性质方面应具有的高强度、低模量、耐腐蚀特点,还具有良好的生物相容性,说明多壁碳纳米管材料在成为未来生物医用新材料方面有潜在应用价值,这些研究结果为以后更深一步的研究提供了实验依据和有用的科学数据。
Nanotechnology is the 20th century, late 80s, early 90s was gradually developed in the forefront of a new cross-disciplinary field, Its rapid development will be activated in the 21st century, all industrial sectors to promote a revolutionary change. Since the discovery of carbon nanotubes (CNTs) has been, CNTs on with its excellent electrical conductivity, mechanical and semiconductor properties of the field of materials science and become a star, has a wide range of applications and commercial value. The study that the CNTs biocompatible better, so its biomedical materials have great application value.
Bio-medical applications, currently targeted research mainly involves surface modification of carbon nanotubes, it has to prevent non-specific adsorption of proteins and identification of specific protein molecules in the function; the use of carbon nanotube electrical properties of the particular shape and to study the cell The in vitro growth and so on. This paper studied the carbon nanotubes (MWCNTs) surface of hemolysis, platelet adhesion and blood protein adsorption as well as cells in the material on the adhesion and growth behavior. The results showed that: Nitrogen-doped and non-nitrogen-doped multi-walled carbon nanotube materials for cell provides a natural extracellular matrix near the built environment, has significantly promoted the proliferation of cell adhesion and long features, with good cell compatibility. Despite the multi-walled carbon nanotubes and carbon paper two kinds of surface adsorption of albumin (Albumin, Alb) as does the size carbon paper adsorption of fibrinogen (Fibrinogen, Fib) and immunoglobulin (Immunoglobulin, IgG) to be less than the multi-walled carbon nanotubes, but multi-walled carbon nanotubes in hemolytic rate and platelet adhesion rate to be lower than the carbon paper, which means that the relatively hydrophobic carbon paper in terms of better multi-walled carbon nanotubes may inhibit platelet adhesion and The formation of thrombus more capable in the carbon paper, with a certain degree of blood compatibility.
The results show that the multi-walled carbon nanotubes have the physical and chemical properties of biological materials should possess high strength, low modulus, corrosion-resistant characteristics, but also has good biocompatibility, the present multi-walled carbon nanotube material in the future bio-medical and new materials have potential application value of these findings for future deeper study provides the experimental basis and useful scientific data.
目前以生物医学应用为目标的相关研究主要涉及对碳纳米管进行表面修饰,使其具有阻止蛋白质非特异性吸附和识别特定蛋白分子的功能；利用碳纳米管的特殊形状和电学性质,研究细胞的体外生长等。本论文研究了碳纸基多壁碳纳米管(MWCNTs)表面的溶血、血小板粘附和血液蛋白质吸附情况以及细胞在该材料上的黏附和生长行为。结果表明：掺氮和不掺氮多壁碳纳米管材料为细胞提供了接近天然细胞外基质的人造环境,具有显著促进细胞粘附和长时间增殖的功能,具有良好的细胞相容性。尽管多壁碳纳米管和碳纸两种材料表面吸附的白蛋白(Albumin, Alb)数量一样,碳纸吸附的纤维蛋白原(Fibrinogen, Fib)和免疫球蛋白(Immunoglobulin, IgG)要少于多壁碳纳米管,但多壁碳纳米管的溶血率和血小板粘附率都要低于碳纸,这意味着相对碳纸而言疏水性更好的多壁碳纳米管可能抑制血小板的粘附和血栓的形成能力更强于碳纸,具有一定的血液相容性。
研究结果表明,这种多壁碳纳米管具备生物材料在物理化学性质方面应具有的高强度、低模量、耐腐蚀特点,还具有良好的生物相容性,说明多壁碳纳米管材料在成为未来生物医用新材料方面有潜在应用价值,这些研究结果为以后更深一步的研究提供了实验依据和有用的科学数据。
Nanotechnology is the 20th century, late 80s, early 90s was gradually developed in the forefront of a new cross-disciplinary field, Its rapid development will be activated in the 21st century, all industrial sectors to promote a revolutionary change. Since the discovery of carbon nanotubes (CNTs) has been, CNTs on with its excellent electrical conductivity, mechanical and semiconductor properties of the field of materials science and become a star, has a wide range of applications and commercial value. The study that the CNTs biocompatible better, so its biomedical materials have great application value.
Bio-medical applications, currently targeted research mainly involves surface modification of carbon nanotubes, it has to prevent non-specific adsorption of proteins and identification of specific protein molecules in the function; the use of carbon nanotube electrical properties of the particular shape and to study the cell The in vitro growth and so on. This paper studied the carbon nanotubes (MWCNTs) surface of hemolysis, platelet adhesion and blood protein adsorption as well as cells in the material on the adhesion and growth behavior. The results showed that: Nitrogen-doped and non-nitrogen-doped multi-walled carbon nanotube materials for cell provides a natural extracellular matrix near the built environment, has significantly promoted the proliferation of cell adhesion and long features, with good cell compatibility. Despite the multi-walled carbon nanotubes and carbon paper two kinds of surface adsorption of albumin (Albumin, Alb) as does the size carbon paper adsorption of fibrinogen (Fibrinogen, Fib) and immunoglobulin (Immunoglobulin, IgG) to be less than the multi-walled carbon nanotubes, but multi-walled carbon nanotubes in hemolytic rate and platelet adhesion rate to be lower than the carbon paper, which means that the relatively hydrophobic carbon paper in terms of better multi-walled carbon nanotubes may inhibit platelet adhesion and The formation of thrombus more capable in the carbon paper, with a certain degree of blood compatibility.
The results show that the multi-walled carbon nanotubes have the physical and chemical properties of biological materials should possess high strength, low modulus, corrosion-resistant characteristics, but also has good biocompatibility, the present multi-walled carbon nanotube material in the future bio-medical and new materials have potential application value of these findings for future deeper study provides the experimental basis and useful scientific data.
引文
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