药物小分子及其与生物大分子相互作用的光谱研究
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摘要
本论文主要应用荧光光谱法和紫外-可见光谱法研究了药物测定及药物小分子与生物大分子的相互作用。
1、应用不同的计算公式研究了小分子探针和生物大分子相互作用的结合参数和结合位点数。
2、通过蛋白内源荧光的猝灭研究了陈皮中两种活性成分同BSA和HSA的相互作用。利用推导的计算结合常数的方程和计算机计算程序得到了橙皮苷和柚皮苷同蛋白作用的结合常数和结合位点数,并与两种常见计算结合常数公式得到的结果进行了比较。结果发现,利用本文所建立的方法计算快速、结果合理。同时,用荧光和吸收光谱法研究了常见四种金属离子对橙皮苷和柚皮苷同两种蛋白相互作用的影响。
3、利用荧光法测定了牛蒡子苷。根据DNA与牛蒡子苷共存时的荧光光谱和紫外光谱。建立了一种新的计算结合常数的方法,得到了牛蒡子苷与DNA的结合常数。为了进一步阐明牛蒡子苷与DNA的结合机理,进行了竞争结合实验和熔解实验。研究结果表明,牛蒡子苷与DNA的结合应当是沟区结合。荧光法的结果还表明单链DNA的猝灭效应大于双链DNA,这意味着牛蒡子苷与DNA的作用不属于嵌入结合。
4、利用荧光光谱法测定了鬼臼毒素、甘草苷和红景天苷,建立了测定这些化合物的方法。研究并选择了它们的最佳激发和荧光发射参数,详尽地考察了乙醇浓度、温度、放置时间、pH值和共存离子对荧光强度的影响。在最佳实验条件下,鬼臼毒素、甘草苷和红景天苷的荧光强度与其浓度分别在0.04～8.29μg·mL~(-1),0.04～8.37μg·mL~(-1)和0.04～18.03μg·mL~(-1)范围内呈良好的线性关系,检测下限分别为0.024,0.017和0.019μg·mL~(-1)。
Protein and deoxyribonucleic acid(DNA) are the basic composition of all organisms,and play important roles in all kinds of life activities.DNA is the carrier of genetic materials which acts the main role to the biological evolution,and protein is the expresser of these genetic materials.The investigation of interaction between small drug molecules with biomacromolecules,such as bovine serum albumin(BSA), human serum albumin(HSA) and DNA,is helpful for us to understand the mechanism of function for drugs and exploit new drugs.
1.The structure and function of BSA,HSA and DNA were introduced,and the study on the interaction between drug molecules and biomacromolecules by fluorescence and UV-visible spectrophotometry,especially by the fluorescence quenching,was summarized.
2.The interaction of the phenylbutazone and ibuprofen with bovine serum albumin was studied by fluorescence spectrometry.The thermodynamic parameters for the binding were obtained by the fluorescence quenching.The reasonable results were obtained by programmed calculation based on the calculating equation established.Compared with the results obtained by the other calculating method,it is proved that the satisfactory results can be obtained by the proposed method.Based on F(o|¨)rster theory of non-radiation energy transfer,the binding distance r of the bovine serum albumin with phenylbutazone and ibuprofen was 1.57 and 1.54 nm, respectively.In addition,the effect of common ions on the binding of the drugs and bovine serum albumin was also examined.The emission intensity of ethidium bromide(EB) in the presence of DNA can be greatly enhanced.The interaction of the DNA with EB was studied and binding parameters,including binding constant and binding sites,were also obtained.
3.Protein has intrinsic fluorescence and can be used as a donor to react with an acceptor.Because of forming of the coordination compound,the protein intrinsic fluorescence can be quenched.Because the bound and unbound drug concentration is difficult to be know during the experiment,the unbound drug concentration is often replaced by the total drug concentration in calculating the binding parameters.The interaction of the two main active components in fruit of Citrus aurantium L.with BSA and HSA was studied by the quenching of intrinsic fluorescence protein.The binding constants and sites of the hesperidin and naringin to proteins were obtained by an improved calculating method and the programmed calculation process.For comparison,other two calculation methods also were applied.The reasonable results were rapidly obtained by programmed calculation process.The△H,△S and△G obtained indicate that the hydrophobic force plays a major role in the interaction of drugs and BSA.The main acting force between naringin and HSA is electrostatic force.However,van der Waals or hydrogen bond plays a major role in the interaction of hesperidin with HSA.Based on the F(o|¨)rster's theory,the binding average distance,r between the protein and drug was evaluated and found to be less than 3 nm.At the same time,the interaction of hesperidin and naringin with BSA and HSA in the presence of four common metal ions was studied by fluorescence and absorption spectrometry.
4.Arctiin was determined by fluorescence method in the various different experiment conditions.At the optimal conditions,the good linear response was observed in the arctiin concentration range from 0.05～10.69μg·mL~(-1).The interaction between arctiin and DNA was also studied by fluorescence and ultraviolet absorption spectrometry.The biomolecule is possessed of a fluorophore and many binding sites, and the drug molecules are quencher in most studies of biomolecule binding with the small molecule.However,in the work DNA is quencher in the interaction of the DNA with EB.A new method for calculating the binding constants and binding sites was developed and the comparison of the propsed method with the conventional method was made.The reasonable results were obtained by the proposed method.The competitive binding and melting temperature(T_m) were carried out to investigate binding mechanism.In addition,the binding characteristics of arctiin and DNA were further studied by fluorescence method and experimental results showed that the fluorescence of arctiin was quenched easier by single stranded DNA(ssDNA) than that by double stranded DNA(dsDNA),which indicated that the interaction between fluoroquinolone and dsDNA does not belong to intercalation.
5.The podophyllotoxin,liquiritin and salidroside were determined by fluorescence spectrometry.The effects of ethanol concentration,temperature,placing time,pH values and foreign substances on the determination of the drugs were examined.At optimal conditions,the good linear responses were observed in the concentration range from 0.04～8.29μg·mL~(-1),0.04～8.37μg·mL~(-1) and 0.04～18.03μg·mL~(-1),for liquiritin and 0.9996 for salidroside and detection limits were 0.024μg·mL~(-1),0.017μg·mL~(-1) and 0.020μg·mL~(-1),respectively.
1、应用不同的计算公式研究了小分子探针和生物大分子相互作用的结合参数和结合位点数。
2、通过蛋白内源荧光的猝灭研究了陈皮中两种活性成分同BSA和HSA的相互作用。利用推导的计算结合常数的方程和计算机计算程序得到了橙皮苷和柚皮苷同蛋白作用的结合常数和结合位点数,并与两种常见计算结合常数公式得到的结果进行了比较。结果发现,利用本文所建立的方法计算快速、结果合理。同时,用荧光和吸收光谱法研究了常见四种金属离子对橙皮苷和柚皮苷同两种蛋白相互作用的影响。
3、利用荧光法测定了牛蒡子苷。根据DNA与牛蒡子苷共存时的荧光光谱和紫外光谱。建立了一种新的计算结合常数的方法,得到了牛蒡子苷与DNA的结合常数。为了进一步阐明牛蒡子苷与DNA的结合机理,进行了竞争结合实验和熔解实验。研究结果表明,牛蒡子苷与DNA的结合应当是沟区结合。荧光法的结果还表明单链DNA的猝灭效应大于双链DNA,这意味着牛蒡子苷与DNA的作用不属于嵌入结合。
4、利用荧光光谱法测定了鬼臼毒素、甘草苷和红景天苷,建立了测定这些化合物的方法。研究并选择了它们的最佳激发和荧光发射参数,详尽地考察了乙醇浓度、温度、放置时间、pH值和共存离子对荧光强度的影响。在最佳实验条件下,鬼臼毒素、甘草苷和红景天苷的荧光强度与其浓度分别在0.04～8.29μg·mL~(-1),0.04～8.37μg·mL~(-1)和0.04～18.03μg·mL~(-1)范围内呈良好的线性关系,检测下限分别为0.024,0.017和0.019μg·mL~(-1)。
Protein and deoxyribonucleic acid(DNA) are the basic composition of all organisms,and play important roles in all kinds of life activities.DNA is the carrier of genetic materials which acts the main role to the biological evolution,and protein is the expresser of these genetic materials.The investigation of interaction between small drug molecules with biomacromolecules,such as bovine serum albumin(BSA), human serum albumin(HSA) and DNA,is helpful for us to understand the mechanism of function for drugs and exploit new drugs.
1.The structure and function of BSA,HSA and DNA were introduced,and the study on the interaction between drug molecules and biomacromolecules by fluorescence and UV-visible spectrophotometry,especially by the fluorescence quenching,was summarized.
2.The interaction of the phenylbutazone and ibuprofen with bovine serum albumin was studied by fluorescence spectrometry.The thermodynamic parameters for the binding were obtained by the fluorescence quenching.The reasonable results were obtained by programmed calculation based on the calculating equation established.Compared with the results obtained by the other calculating method,it is proved that the satisfactory results can be obtained by the proposed method.Based on F(o|¨)rster theory of non-radiation energy transfer,the binding distance r of the bovine serum albumin with phenylbutazone and ibuprofen was 1.57 and 1.54 nm, respectively.In addition,the effect of common ions on the binding of the drugs and bovine serum albumin was also examined.The emission intensity of ethidium bromide(EB) in the presence of DNA can be greatly enhanced.The interaction of the DNA with EB was studied and binding parameters,including binding constant and binding sites,were also obtained.
3.Protein has intrinsic fluorescence and can be used as a donor to react with an acceptor.Because of forming of the coordination compound,the protein intrinsic fluorescence can be quenched.Because the bound and unbound drug concentration is difficult to be know during the experiment,the unbound drug concentration is often replaced by the total drug concentration in calculating the binding parameters.The interaction of the two main active components in fruit of Citrus aurantium L.with BSA and HSA was studied by the quenching of intrinsic fluorescence protein.The binding constants and sites of the hesperidin and naringin to proteins were obtained by an improved calculating method and the programmed calculation process.For comparison,other two calculation methods also were applied.The reasonable results were rapidly obtained by programmed calculation process.The△H,△S and△G obtained indicate that the hydrophobic force plays a major role in the interaction of drugs and BSA.The main acting force between naringin and HSA is electrostatic force.However,van der Waals or hydrogen bond plays a major role in the interaction of hesperidin with HSA.Based on the F(o|¨)rster's theory,the binding average distance,r between the protein and drug was evaluated and found to be less than 3 nm.At the same time,the interaction of hesperidin and naringin with BSA and HSA in the presence of four common metal ions was studied by fluorescence and absorption spectrometry.
4.Arctiin was determined by fluorescence method in the various different experiment conditions.At the optimal conditions,the good linear response was observed in the arctiin concentration range from 0.05～10.69μg·mL~(-1).The interaction between arctiin and DNA was also studied by fluorescence and ultraviolet absorption spectrometry.The biomolecule is possessed of a fluorophore and many binding sites, and the drug molecules are quencher in most studies of biomolecule binding with the small molecule.However,in the work DNA is quencher in the interaction of the DNA with EB.A new method for calculating the binding constants and binding sites was developed and the comparison of the propsed method with the conventional method was made.The reasonable results were obtained by the proposed method.The competitive binding and melting temperature(T_m) were carried out to investigate binding mechanism.In addition,the binding characteristics of arctiin and DNA were further studied by fluorescence method and experimental results showed that the fluorescence of arctiin was quenched easier by single stranded DNA(ssDNA) than that by double stranded DNA(dsDNA),which indicated that the interaction between fluoroquinolone and dsDNA does not belong to intercalation.
5.The podophyllotoxin,liquiritin and salidroside were determined by fluorescence spectrometry.The effects of ethanol concentration,temperature,placing time,pH values and foreign substances on the determination of the drugs were examined.At optimal conditions,the good linear responses were observed in the concentration range from 0.04～8.29μg·mL~(-1),0.04～8.37μg·mL~(-1) and 0.04～18.03μg·mL~(-1),for liquiritin and 0.9996 for salidroside and detection limits were 0.024μg·mL~(-1),0.017μg·mL~(-1) and 0.020μg·mL~(-1),respectively.
引文
[1]CARTER D,CHANG B,HO J X,et al.Preliminary crystallographic studies of four crystal form of serum albumins[J].Eur.J.Biochem.,1994,226:1049-1052.
[2]CARTER D,HO J X.Structure and ligand binding properties of human serum albumin[J].Adv Protein Chem.,1994,45:153-203.
[3]BROWN J R,SHOCKLEY P.Lipid-Protein Interactions,vol.1,Wiley,New York,1982.
[4]CARTER D C,HE X M,MUNSON S H,et al.Three-dimensional structure of human serum albumin[J].Science,1989,244(4909):1195-1198.
[5]Carter D C,He X M.Structure of human serum albumin[J].Science,1990,249(4966):302-303.
[6]HE X M,CARTER D C.Atomic structure and chemistry of human serum albumin[J].Nature,1992,358:209-215.
[7]KRAGH-HANSEN U.Molecular aspects of ligand binding to serum albumin[J].Pharmcol.Rev.,1981,33:17-53.
[8]PETERS T.Serum albumin.Adv.Protein Chem.,1985,37:161-245.
[9]SUDLOW G,BIRKETT D J,WADE D N.The Characterization of Two Special Drug Binding Sites on Human Serum Albumin[J].Mol.Pharmacol.,1975,11(6):824-832.
[10]FEHSKE K J,M(U|¨)LLER W E,WOLLERT U.The location of drug binding sites in human serum albumin[J].Biochem.Pharmacol.,1981,30:687-592.
[11]HE X M,CARTER D C.Atomic structure and chemistry of human serum albumin[J].Nature,1992,358(2):209-215.
[12]SJ(O|¨)HOLM I,EKMAN B,KOBER A,et al.Binding of drugs to human serum albumins-Ⅸ.The specificity of three binding sites as studied with albumin immobilised in microparticles[J].Mol.Pharmacol.,1979,16(3):767-777.
[13]FEHSKE K J,SCHL(A|¨)FER U,WOLLERT U,et al.Characterization of an important drug binding area on human serum albumin including the high-affinity binding sites of warfarin and azapropazone [J].Mol.Pharmacol.,1982,21(2):387-393.
[14]SUDLOW G,BIRKETT D J,WADE D N.Further characterization of specific drug binding sites on human serum albumin[J],Mol.Pharmacol.,1976,12,1052-1061.
[15]徐岩,沈含熙,黄汉国.利用标记药物布洛芬及保泰松研究萘啶酸与血清白蛋白的结合作[J].高等化学学报,1996,17(12):1855-1858.
[16]YAMASAKI K,MARUYAMA T,KRAGH-HANSEN U,ET AL.Characterization of site I on human serum albumin:concept about the structure of a drug binding site[J].Biochim.Biophys.Acta,1996,1295(2):147-157.
[17]BARONI S,MATTU M,VANNINI A,et al.Effect of ibuprofen and warfarin on the allosteric properties of heine-human serum albumin.A spectroscopic Study[J].Eur.J.Biochem.,2001,268:6214-6220.
[18]WILTING J,van der GIESEN W F,JANSSEN L H,et al.The effect of albumin conformation on the binding of warfarin to human serum albumin.The dependence of the binding of warfarin to human serum albumin on the hydrogen,calcium,and chloride ion concentrations as studied by circular dichroism,fluorescence,and equilibrium dialysis[J].J.Biol.Chem.,1980,255:3032-3037.
[19]PETITPAS I,BHATTACHARYA A A,TWINE S M,et al.Crystal structure analysis of warfarin binding to human serum albumin[J].J.Biol.Chem.,2001,276(25):22804-22809.
[20]KRAGH-HANSEN U,CHUANG V Y G,OTAGIRI M.Practical aspects of the ligand-binding and enzymatic properties of human serum albumin[J].Biological & Pharmaceutical Bulletin,2002,25(6):695-704.
[21]MANDULA H,PAREPALLY J M R,FENG R,ET AL.Role of site-specic binding to plasma albumin in drug availability to brain[J].J.Pharmacol.Exp.Yher.,2006,317(2):667-675.
[22]NI Y N,SU S J,KOKOT S.Spectrofluorimetric studies on the binding of salicylic acid to bovine serum albumin using warfarin and ibuprofen as site markers with the aid of parallel factor analysis[J].Anal.Chim.Acta,2006,580(2):206-215.
[23]DUFOUR C,DANGLES O.Flavonoid-serum albumin complexation:determination of binding constants and binding sites by fluorescence spectroscopy[J].Biochim.Biophys.Acta,2005,1721(1):164-173.
[24]BOS O J M,REMIJN J P M,FISCHER M J E,et al.Location and characterization of the warfarin binding site of human serum albumin,A comparative study of two large fragments[J].Biochem.Pharmacol.,1988,37:3905-3909.
[25]KOSA T,MARUYAMA T,OTAGIRI M.Species differences of serum albumin:I.Drug binding sites[J].Pharm.Res.,1997,14(11):1607-1612.
[26]WATSON J D,CRICK F H C.Molecular structure of nucleic acids-a structure for deoxyribose nucleic acid[J].Nature,1953,171:737-738.
[27]FRATINI A V,KOPKA M L,DREW H,et al.J.Reversible bending and helix geometry in a B-DNA dodecamer:CGCGAATTBrCGCG[J].Biol.Chem.,1982,257:14686-14707.
[28]NELSON H C,FINCH J T,LUISI B F,et al.The Structure of an Oligo(Da).Oligo(dT) tract and its biological implications[J].Nature,1987,330:221-226.
[29]PULLMAN B.Electrostatics of Polymorphic DNA[J].J.Biomol.Struct.Dyn.,1983,1(3):773-794.
[30]KOPKA M L,YOON C,GOODSELL D,et al.The molecular origin of DNA-drug specificity in netropsin and distamycin[J].Proc.Natl.Acad.Sci.,1985,82(5):1376-1378.
[31]LEE K H,BENSON D R,KUCZERA K.Transitions from α to π helix observed inmolecular dynamics simulations of synthetic peptides[J].Biochem.,2000,39(45):13737-13747.
[32]LAMPELA O,JUFFERA H,RAUK A.Conformational analysis of glutathione in aqueous solution with molecular dynamics[J].J.Phy.Chem.A,2003,107(43):9208-9220.
[33]VAMAI P,LAVERY R.Base flipping in DNA:pathways and energetics studied with molecular dynamic simulations[J].J.Am.Chem.Soc.,2002,124(25):7272-7273.
[34]JEREMY B JOHN T,LUBERT S.Biochemistry[M].5th ed.New York:W.H.Freeman and Company,2002 Chap.1.The Molecular Design of Life.In:NCBI online bookshelf.
[35]张荣.生化模型分子及生物小分子水溶液弱相互作用研究[C].博士学位论文杭州浙江大学,2006.
[36]周新,汪子明,邹明强等.亲和毛细管电泳法测定3种黄酮类化合物与人血清白蛋白的结合常数[J].吉林大学学报,2005,43(5):669-672.
[37]郭明,严建伟,俞庆森等.加替沙星与牛血清白蛋白的结合反应研究[J].物理化学学报,2004,20(2):202-206.
[38]邬春华,吕元琦,袁倬斌.大黄酸与牛血清白蛋白相互作用的电化学研究[J].分析测试学报,2004,23(3):73-75.
[39]龙德武,刘传银,赵鸿雁等.铜Ⅱ-三氮唑偶氮络合物与脱氧核糖核酸相互作用的电化学行为[J].分析化学,2002,30(10):1250-1253.
[40]李天剑,沈含熙,罗云敬.乙基紫标记分光光度法测定脱氧核糖核酸[J].分析化学,1998,26(11):1372-1375.
[41]宋功武,方光荣,冯健.甲基紫核酸分子相互作用的紫外-可见光谱及其分析应用[J].分析化学,2000,28(1):128-132.
[42]俞英,程晓伟.牛血清白蛋白与酸性品红的结合反应[J].分析化学,2000,28(5):583-586.
[43]王春,吴秋华,王志等.槲皮素与牛血清白蛋白相互作用的研究[J].光谱学与光谱分析,2006,26(9):1672-1675.
[44]KELLY S M,JESS T J,PRICE N C.How to study proteins by circular dichroism[J].Biochimica et Biophysica Acta,2005,1751(2):119-139.
[45]梁毅著.结构生物化学[M].北京:科学出版社,2005,307.
[46]GERBANOWSKI A,MALABAT C,RABILLER C,et al.Grafting of aliphatic and aromatic probes on rapeseed 2S and 12S proteins:influence on their structural and physicochemical properties [J].J.Agric.Food Chem.,1999,47:5218-5226.
[47]杨频,高飞编著.生物无机化学原理[M].北京:科学出版社,2002.
[48]沈星灿,梁宏,何锡文等.圆二色光谱分析蛋白质构象的方法及研究进展[J].分析化学,2004,4(3):388-394.
[49]于海英,程秀民,王晓坤.圆二色光谱及其在药物研究方面的应用[J].光谱实验室,2007,24(5):877-885.
[50]Provencher S W,Glockner J.Estimation of globular protein secondary structure from circular dichroism[J].Biochem.,1981,20:33-37.
[51]CHIGNELL C F.Optical studies of drug-protein complexes:Ⅲ.interaction of flufenarnic acid and other N-Arylanthranilates with serum albumin[J].Mol.Pharmacol.,1969,5:455-462.
[52]Chignell C.F.Optical Studies of drug-protein complexes:Ⅱ.interaction ofphenylbutazone and its analogues with human serum albumin[J].Mol.Pharmacol.,1969,5:244-252.
[53]HWANGBO H J,YUN B H,CHA J S,et al.Enantioselective binding of S-and R-ofloxacin to V arious synthetic polynucleotides[J].European Journal of Pharmaceutical Sciences,2003,18(2):197-203.
[54]KANDAGAL P B,SEETHARAMAPPA J,ASHOKA S,et al.Study of the interaction between doxepin hydrochloride and bovine serum albumin by spectroscopic techniques[J].Int.J.Biol.Macromol,2006,39(4-5):234-239.
[55]王建林,付连春,周实武等.维生素B_6与人血清白蛋白的相互作用[J].光谱学与光谱分析,2005,25(6):912-915.
[56]RODGER A,BLAGBROUGH I S,ADLAM G,et al.DNA binding of a spermine derivative:spectroscopic study of anthracene-9-carbonyl-N1-spermine with poly[d(G-C).d(G-C)]and poly[d (A-T).d(A-T)][J].Biopolymers,1994,34:1583-1593.
[57]王云普,萧耀南,王荣民.水溶性高分子金属钴卟啉络合物的合成及其与DNA作用的研究[J].西北师范大学学报(自然科学版),2000,369(1):40-44.
[58]THEOPHANIDES T.Fourier Transform Infrared Spectra of Calf Thymus DNA and its Reactions with the Anticancer Drug Cisplatin[J].Appl.Spectrosc.,1981,35:461-465.
[59]WILSON W D.Intercalation Chemistry[M].Academic Press,1982,445-501.
[60]王树玲,于俊生.表面增强拉曼光谱研究小檗碱与DNA的相互作用[J].高等学校化学学报.2002,23(9):1676-1679.
[61]GAMBARI R,FERIOTTO G,RUTIGLIANO C,et al.Biospecific Interaction Analysis(BIA) of Low-Molecular Weight DNA-Binding Drugs[J].J.Pharmacol.Exp.Ther.,2000 294:370-377.
[62]MINUNNI M,TOMBELLI S,MASCINI M,et al.An optical DNA-based biosensor for the analysis of bioactive constituents with application in drug and herbal drug screening[J].Talanta,2005,65:578-585.
[63]AHMAD A,RAMAKRISHNAN A,MCLEAN M A,et al.Use of surface plasmon resonance biosensor technology as a possible alternative to detect differences in binding of enantiomeric drug compounds to immobilized albumins.Biosensors and Bioelectronics,2003,18:399-404.
[64]DANELIAN E,KARLEN A,KARLSSON R,et al.SPR biosensor studies of the direct interaction between 27 drugs and a liposome surface:Correlation with fraction absorbed in humans.J.Med.Chem.,2000,43:2083-2086.
[65]NEDELKOV D,NELSON R W.Delineating protein-protein interations via biomolecular interaction analysis-mass spectrometry[J].J.Mol.Recognit.,2003,16(1):9-14.
[66]KIKUCHI J,FURUKAWA Y,HAYASHI N.Identification of novel P53-binding proteins by biomolecular interaction analysis combined with tandem mass spectrometry[J].Mol.Biotechnol.,2003,23(3):203-212.
[67]潘祖,刘义庆,刘小玲.药物荧光分析法研究进展[J].武汉大学学报(自然科学版),2000,46(16):674-680.
[68]LAKOWICA J R,WEBER G.Quenching of fluorescence by oxygen.Probe for structural fluctuations in macromolecules[J].Biochem.,1973,12:4161-4170.
[69]EFTINK M R.In Biophysical and Biochemical Aspects of Fluorescence Spectroscopy(ed.Dewey T G,)[M].Plenum Press,New York,1991,1-41.
[70]WARE W R.Oxygen quenching of fluorescence in solution:an experimental study of the diffusion process[J].J.Phys.Chem.,1962,66:455-458.
[71]LAKOWICZ J R.Principles of Fluorescence Spectroscopy,second ed.[M].New York:Plenum Press,1999,237-259.
[72]LAKOWICZ J.R.Principles of Fluorescence Spectroscopy,third ed.[M].Springer,2006,278-282.
[73]陈晓波,李崧.铜、钠和钼离子与牛血清白蛋白作用的荧光光谱研究[J].光谱学与光谱分析,2006,26(2):309-312.
[74]TWINE S M,GORE M G,MORTON P,et al.Mechanism of binding of warfarin enantiomers to recombinant domains of human albumin[J].Arch.Biochem.Biophys.,2003,414(1):83-90.
[75]易长海,颜承农,上官云凤等.苯噻啶与蛋白质作用特征的热力学研究[J].华中师范大学学报,自然科学版2002,32(3):317-319.
[76]玄光善,吴效楠,李玉平.荧光光谱法研究乙酰水杨酸和牛血清蛋白的相互作用[J].光谱实验室,2005,22(4):861-864.
[77]王彦卿,张红梅,张朝平.荧光光谱法研究茶碱与血红蛋白的相互作用[J].化学试剂,2006,28(3):156-158.
[78]盛良全,童红武,张淑萍等.烟碱与人血清蛋白相互作用的光谱研究[J].分析化学,2006,34:S203-S206.
[79]刘永明,王进军.脱镁叶绿酸α-甲酯的合成及对牛血清白蛋白的结合作用[J].光谱学与光谱学分析,2005,25(4):747-750.
[80]LEHRER S S,Solute perturbation of protein fluorescence[J].Biochem.,1971,10:3254-3263.
[81]TIMASEFF S N.Thermodynamics of protein interactions[M].In:Proteins of biological fluids (Ed.Peeters H),Pergamon Press,Oxford,1972,511-519.
[82]CARTER D C,CHANG B,HO J X,et al.Preliminary Crystallographic Studies of Four Crystals Forms of Serum Albumin[J].Eur.J.Biochem.,1994,226:1049-1052.
[83]刘家琴.中药活性组分与蛋白质相互作用研究[D].兰州:兰州大学,2004.
[84]ASHOKA S,SEETHARAMAPPA J,KANDAGAL P B,et al.Investigation of the interaction between trazodone hydrochloride and bovine serum albumin[J].Journal of Luminescence,2006,121:179-186.
[85]SHAIKH S M T,SEETHARAMAPPA J,ASHOKA S,et al.Fluorescent homodimer styrylcyanines:synthesis and spectral-luminescent studies in nucleic acids and protein complexes[J].Dyes and Pigments 2007,73:211-216.
[86]WU P,BRAND L.Review-Resonance energy transfer[J].Meth Appl Biochem,1994,218:1-13.
[87]F(O|¨)RSTER T.Intramolecular energy migration and fluorescence[J].Ann.Phys.,1948,2:5-75.
[88]F(O|¨)RSTER T,SINANOGLU O.Modern Quantum Chemisby[M].New York:Academic Press,1966.
[89]STRYER L,HAUGLAND R.Energy transfer:a spectroscopic ruler[M].Proc.Natl.Acad.Sci.USA,1967,58:719-726.
[90]陈国珍,黄贤智,许金钩等.荧光分析法(第二版)[M].科学出版社,北京,1990,123.
[91]马贵斌,高尺,任斌知等.荧光法研究药物分子与人血清白蛋白的结合作用[J].化学学报,1995,53(12):1193-1197.
[92]HEIM R,PRASHER D C,TSIEN R Y.Wavelength mutations and posttranslationalauxidation of green fluorescent protein[J].Proc.Natl.Acad.Sci.USA,1994,91:12501-12504.
[93]SUZUKIDA M,LE H P,SHAHID F,et al.Resonance energy transfer between cysteine-34 and tryptophan-214 in human serum albumin.Distance measurements as a function of pH[J].Biochem.,1983,22:2415-2420.
[94]汪海林,邹汉法,张玉奎.微透析-液相色谱研究药物与蛋白竞争结合[J].中国科学辑,1998,28(1):71-77.
[95]KRAAK J C,BUSCH S,POPPE H.Study of protein-drug binding using capillary zone electrophoresis[J].J.Chromatogr.A,1992,608(1-2):257-264.
[96]SCATCHARD G,SCHEIBERG I H,ARMSTRONG S H.Physical chemistry of protein solutions Ⅳ:the combination of human serum albumin with Chloride Ion[J].J.Am.Chem.Soc.,1950,72(1):535-541.
[97]SCATCHARD G.The attractions of protein for small molecules and ions[J].Ann.N.Y.Acad.Sci.,1949,51(4):660-672.
[98]YANG P,YANG M M,YANG B S.The fluorescence enhancement and the action between donor and acceptor[J].Chin.J.Chem.,1996,14(2):109-113.
[99]杨曼曼,杨频,席小莉.荧光染料探针与蛋白质结合的研究[J].科学通报,1997,42(12):1276-1279.
[100]JAMES D M,PETER H.Theoretical aspects of DNA-Protein interactions:Co-operative and Non Co-operative binding of large ligands to a one-demensional homogeneous lattice[J].J.Mol.Biol.,1974,86:469-489.
[101]CONGDON R W,MUTH G W,SPLITTGERBER A G.The binding interaction of coomassie blue with proteins[J].Anal.Biochem.,1993,213:407-413.
[102]KLOTZ I M,HUNSTONE D L.Properties of graphical representations of multiple classes of binding sites[J].Biochem.,1971,10:3065-3069.
[103]McGHEE J D,von HIPPEL P H.Theoretical aspects of DNA-Protein interactions:Co-operative and Non Co-o-perative binding of large ligands to a one-demensional homogeneous lattice[J].J.Mol.Biol.,1974,86:469-489.
[104]ReLNHARDT C G,KRUGH T R.A comparative study of ethdium bromide complexe with dinucleotide and DNA:direct evidence for intercalation and nucldc acid sequence preferences[J].Biochem.,1978,17:4845-4854.
[105]LEPECQ J B,PAOLETTI C.A fluorescent complex between ethidium bromide and nucleic acids.Physical-chemical characterization[J].J.Mol.Biol.,1967,27(1):87-106.
[106]YI P G,YU Q S,SHANG Z C,et al.The reaction mechanism between ofloxacin and bovine serum albumin[J].Acta Pharm.Sin.,2000,35:774-777.
[107]ALAIN M,MICHEL B,MICHEL A.How to illustrate ligand-protein binding in a class experiment:An elementary fluorescent assay[J].J.Chem.Edu.,1986,63(4):365-366.
[108]张勇,张贵珠,王月梅等.光谱法研究丝裂霉素、血清白蛋白以及金属离子间的相互作用[J].分析科学学报,2000,16(6):44-49.
[109]ZHAO H W,GE M,ZHANG Z X,et al.Spectroscopic studies on the interaction between riboflavin and albumins[J].Spectrochim.Acta,Part A,2006,65(3-4):811-817.
[110]FENG X Z,LIN Z,YANG L J,et al.Piezoelectric quartz crystal biosensors(Review)[J].Talanta,1998,47:1223-1236.
[111]BARIK A,PRIYADARSINI K I,MOHAN H.Photophysical studies on binding of curcumin to bovine serum albumin[J].Photochem.Photobiol.,2003,77:597-603.
[112]Bi S Y,Zhang H Q,Qiao C Y,et al.Studies of interaction of emodin and DNA in the presence of ethidium bromide by spectroscopic method[J].Spectrochim.Acta.Part A,2008,69:123-129.
[113]JONES M N.Surfactant interactions with biomembranes and proteins[J].Chem.Soc.Rev.,1992,21:127-136.
[114]HALFMAN C J,NISHIDA T.Measuring the binding of small molecules to protein from binding-induced alterations of physical-chemical properties[J].Biochem.,1972,11(18):3493-3498.
[115]JONES M N,CHAPMAN D.Micelles,Monolayers and Biomembranes[M].Wiley-Riss,New York,1995,ch.6,143-179.
[116]ROSENTHANL H R.Graphic method for the determination and presentation of binding parameters in a complex system[J].Anal.Biochem.,1967,20,525-532.
[117]SUR S S,RABBANI L D,LIBMANL L,ET AL.Fluorescence studies of native and modified neurophysms.Efects of piptides and pH[J].Biochem.,1979,18(6):1026-1035.
[118]LAKOWICZ J R.Principles of Fluorescence Spectroscopy[M],2nd Ed.,Kluwer Academic/Plenum Publishers,New York,1999.
[119]LERMAN L S.Structural considerations in the interaction of DNA and acridines[J].J.Mol.Biol.,1961,3:18-30.
[120]STEINER R T,WEINRYB L.Excited States of Protein and Nucleic Acid[M].Plenum Press,New York,1971,40.
[121]徐岩,黄汉国,沈含熙.喹诺酮类药物对人血清白蛋白的荧光猝灭研究[J].分析化学,1998,12:1494-1497.
[122]徐岩,沈含熙,黄汉国.利用标记药物布洛芬及保泰松研究萘啶酸与血清白蛋白的结合作用[J].高等化学学报,1996,17(12):1855-1858.
[123]张霞,倪永年.荧光光谱法研究盐酸曲唑酮与BSA的相互作用[C].南昌大学学报增刊(第九届中国化学会分析化学年会暨地九届全国原子光谱学术会议论文集),2006,30:337-338.
[124]TIAN J N,LIU J Q,XIE J Q,et.al.Binding of wogonin to human serum albumin.A common binding site ofwogonin in Subdomain ⅡA[J].J.Photochem.Photoboil.B:Biology,2004,74:39-45.
[125]DUFOUR C,DANGLES O.Flavonoid-serum albumin complexation:determination of binding constants and binding sites by flurescence specteoscopy[J].Biochim.Biophys.Acta.,2005,1721(1):164-173.
[126]MORENO F,GONZALEZ-JIMENEZ J.Binding of the Promen fluorescent probe to human serum albumin:a fluorescence spectroscopic study[J].Chem.Biol.Interact.,1999,121(2):237-252.
[127]SOLTES L,MACH M.Estimation of drug-protein binding parameters on assuming the validity of thermodynamic equilibrium[J].J.Chromatogr.B.,2002,768:113-119.
[128]张保林,王文清,白凤莲.蒽醌及黄酮类化合物与人血清白蛋白的结合反应研究[J].高等学校化学学报,1994,15(3):373-378.
[129]KOWSKA A S.Interaction of drugs with bovine and human serum albumin[J].J.Mol.Struct.,2002,614:227-232.
[130]ROSS D P,SUBRAMANIAN S.Thermodynamics of protein association reactions:forces contributing to stability[J].Biochem,1981,20:3096-3102.
[131]KLOTZ I M,URQUHART J M.The binding of organic ions by proteins.Effect of temperature [J].J.Am.Chem.Soc.,1949,71:847-851.
[132]TANFORD C.Physical Chemistry of Macromolecules[M].John Wiley and Sons.New York,1971,526
[133]TANFORD C.The Hydrophobic Effect:Formation of Micciies and Biological Membranes[M].New York:John Wiley and Sons,1973,12.
[134]MOHAMMED H R,TORU M,TOMOKO O,et.al.Study of interaction of carprofen and its enantiomers with human serum albumin-I mechanism of binding studied by dialysis and spectroscopic methods[J].Biochem.Pharm.,1993,46(10):1721-1731.
[135]ORWCORA J,BOCHS B.In silico prediction of drug-binding strengths to human serum albumin[J].J.Chromatogr.B:Biomed.Appl.,1996,677(1):1-28.
[136]GONZALEZ J Z,ACQUOTEH J,CAYRE I.Fluorescence quenching studies on binding fluoreno-9-spiro-oxazolidinedione to human serum albumin[J].Chem.Biol.Interactions,1992,84:221-228.
[137]LLOYD J B F.Synchronized excitation of fluorescence emission spectra[J].Nature(Phys.Sci.),1971,231:64-65.
[138]MILLER J N.Recent advances in molecular luminescence analysis[J].Proc.Anal.Div.Chem.Soc.,1979,16:203-208.
[139]BRUSTEIN E A,VEDENKINA N S,IRKOVA M N.Fluorescence and the location of tryptophan residues in protein molecules[J].Photochem.Photobiol.,1973,18:263-279.
[140]陈晓波,康栋国,李崧等.利福平与人血清白蛋白作用的荧光光谱[J].光谱学与光谱分析, 2006,26(4):674-677.
[141]卞伟,卫艳丽,王亚萍等.荧光法研究咖啡因和茶碱与牛血清白蛋白相互作用[J].光谱学与光谱分析,2006,26(3):505-508.
[142]毕淑云,丁兰,宋大干等.几种抗生素与人血清白蛋白结合反应的研究[J].化学学报,2005.63:2169-2173.
[143]BI S Y,SONG D Q,TIAN Y,et al.Molecular spectroscopic study on the interaction of tetracyclines with serum albumins[J].Spectrochim.Acta,Part A,2005,61:629-636.
[144]毕淑云,宋大干,魏士刚等.用荧光猝灭法研究人血清白蛋白与吲哚美辛的结合反应[J].吉林大学学报(理学版),2006,44:493-496.
[145]QI Z D,ZHANG Y,LIAO F L,et al.Probing the binding of morin to human serum albumin by optical spectroscopy[J].J.Pharm.Biomed.Anal.,2008,46:699-706.
[146]QI Z D,ZHOU B,XIAO Q,et al.Interaction of rofecoxib with human serum albumin:Determination of binding constants and the binding site by spectroscopic methods[J].J.Photochem.Photobiol.A,2008,193:81-88.
[147]SULKOWSKA A,MACIAZEK-JURCZYK M,BOJKO B,et al.Competitive binding of phenylbutazone and colchicine to serum albumin in multidrug therapy:A spectroscopic study[J].J.Mol.Struct.,2008,881:97-106.
[148]王家恒,胡忠阳,张江义等.诺氟沙星-Tb~(3+)-牛血清白蛋白相互作用的荧光光谱研究[J].分析试验室,2008,27:81-83.
[149]杨曼曼,席小莉,杨频.用荧光猝灭和荧光加强两种理论研究喹诺酮类新药与白蛋白的作用[J].高等学校化学学报,2006,27(4):687-691.
[150]张国文,阙青民,潘军辉.山姜素与脱氧核糖核酸的相互识别研究[J].光谱学与光谱分析,2008,28(8):1910-1913.
[151]BI S Y,QIAO C Y,SONG D Q,et al.Study of interactions of flavonoids with DNA using acridine orange as a fluorescence probe[J].Sens.Actuators B,2006,119:199-208.
[152]费丹,王兴明,黎泓波等.亚甲基蓝与鲱鱼精DNA相互作用的光谱法研究[J].化学学报,2008,66:443-448.
[153]徐伟民,王志颖,干宁.氢氯噻嗪与DNA相互作用的光谱研究[J].光谱实验室,2006,23:509-512.
[1]QI Z D,ZHOU B,XIAO Q,et al.Interaction of rofecoxib with human serum albumin: Determination of binding constants and the binding site by spectroscopic methods[J].J.Photochem.Photobiol.,A,2008,193:81-88.
[2]VALLNER J J.Binding of drugs by albumin and plasma protein[J].J.Pharm.Sci.,1977,66:447-465.
[3]SUDLOW G,BIRKETT D J,WADE D N.The Characterization of Two Specific Drug Binding Sites on Human Serum Albumin[J].Mol.Pharmacol.,1975,11:824-832.
[4]徐岩,沈含熙,黄汉国.利用标记药物布洛芬及保泰松研究萘啶酸与血清白蛋白的结合作用[J].高等学校化学学报,1996,12(7):1855-1858.
[5]QI Z D,ZHANG Y,LIAO F L,et al.Probing the binding of morin to human serum albumin by optical spectroscopy[J].J.Pharm.Biomed.Anal.,2008,46:699-706.
[6]LERMAN L S.Structural considerations in the interaction of DNA and acridines[J].J.Mol.Biol.,1961,3:18-30.
[7]STEINER R T,WEINRYB L.Excited States of Protein and Nucleic Acid[M].Plenum Press,New York,1971,40.
[8]CHEN G Z.Fluorescence Analytical Method[M].2nd Ed.,Science Press,Beijing,1990:122.
[9]DEWEY T G.Biophysical and Biochemical Aspects of Fluorescence Spectroscopy[M].Plenum,New York,1991:1-41.
[10]LAKOWICA J R,WEBER G.Quenching of fluorescence by oxygen.Probe for structural fluctuations in macromolecules[J].Biochem.,1973,12:4161-4170.
[11]WARE W R.Oxygenquenchixg of fluorescence in solution:ax experimental study of the diffusion process[J].J.Phys.Chem.,1962,66:455-458.
[12]SILVA D,CORTEZ C M,LOURO S R W.Chlorpromazine interactions to sera albumins:A study by the quenching of fluorescence[J].Spectrochim.Acta Part A,2004,60:1215-1223.
[13]FENG X Z,LIN Z,YANG L J,et al.Investigation of the Interaction between Acridine Orange and Bovine Serum Albumin[J].Talanta,1998,47:1223-1229.
[14]LAKOS Z,SZARKA A,KOSZORBS L,et al.Quenching-resolved emission anisotropy:a steady state fluorescence method to study protein dynamics[J].J.Photochem.Photobiol.B,1995,27:55-60.
[15]YI P G,YU Q S,SHANG Z C,et al.The reaction mechanism between ofloxacin and bovine serum albumin[J].Acta.Pharm.Sinica.,2000,35:774-777.
[16]BI S Y,QIAO C Y,SONG D Q,et al.Study of interactions of flavonoids with DNA using acridine orange as a fluorescence probe[J].Sensors and Actuators B,2006,119:199-208.
[17]EPPS D E,RAUB T J,KEZDY F J.A general,wide-rage spectrofluorometric method for measuring the site-specific affinities of drugs toward human serum albumin[J].Anal.Biochem.,1995,227(2):342-350.
[18]HAQ I.Thermodynamics of drug-DNA interactions[J].Arch.Biochem.Biophys.,2002,403:1-15.
[19]WYMAN T B,NICOL F,ZELPHATI O,et al.Design,synthesis,and characterization of a cationic peptide that binds to nucleic acids and permeabilizes bilayers[J].Biochem.,1997,36(10):3008-3017.
[20]IZUMRUDOV V A,ZHIRYAKOVA M V,KUDAIBERGENOV S E.Ethidium Bromide as a Promising Probe for Studying DNA Interaction with Cationic Amphiphiles and Stability of the Resulting Compleces[J].Biopolymers,2000,52,94-108.
[21]LLERES D,CLAMME J P,DAUTY E,et al.Investigation of the stability of dimeric cationic surfactant/DNA complexes and their interaction with model membrane systems[J].Langmuir,2002,18(26):10340-10347.
[22]IZUMRUDOV V A,ZHIRYKOVA M V,GOULKO A A.Ethidium bromide as a promising probe for studying DNA interaction with cationic amphiphiles and stability of the resulting complexes [J].Langmuir,2002,18(26):10348-10356.
[23]REICHMANN M E,RICE S A,THOMAS C A,et al.A further examination of the molecular weight and size of deoxypentose nucleic acid[J].J.Am.Chem.Soc.,1954,76:3047-3053.
[24]Marmur J.A procedure for the isolation of deoxyribonucleic acid from microorganism[J].J.Mol.Biol.1961,3:208-218.
[25]MAHLER H R,KLINE B,MEHROTRA B D.Some observations on the hypochromism of DNA [J].J.Mol.Biol.,1964,9:801-811.
[26]LONG E C,BARTON J K.Spectroscopic and electrochemical studies of the interaction between fuchsin basic DNA[J].Account Chem.Res.,1990,23(9):271-273.
[27]UDENFRIEND S,ZALTZMAN P.Fluorescence characteristics of purines,pyrimidines,and their derivatives:measurement of guanine in nucleic acid hydrolyzates[J].Anal.Biochem.,1962,3:49-59.
[28]靳兰,杨频,李青山.荧光法研究手性金属配合物与DNA的作用机理[J].高等学校化学学报,1996,17(9):1345-1348.
[29] ROSENTHAL H E. A graphic method for the determination and presentation of binding parameters in a complex system [J]. Anal. Biochem., 1967, 20 (3): 525-532.
[30] MESPLET N, MORIN P, RIBET J P. Spectrofluorimetric study of eflucimibe-y-cyclodextrin inclusion complex [J]. Eur. J. Pharm. Biopharm., 2005, 59: 523-526.
[1]贾秀荣,周济桂,廉乐贤等.橙皮甙与橙皮甙铜配合物生物活性的比较研究[J].广东微量元素科学,1996,3(2):53-56.
[2]KUNTIC V,BLAGOJEVI S,MALESEV D,et al.Spectrophotometrie investigation of the Cu (Ⅱ)-hesperidin complex[J].Pharmazie,1999,54(7):548.
[3]MALESEV D,RADOVIE Z,KUNTIC V,et al.Speetrophotometrie determination of hesperidin by Al(Ⅲ)-hesperidin complex in water-methanol solution[J].Anal.Lett.,1997,30(5):917-926.
[4]张甘良,汪钊,鄢洪德.生物类黄酮化合物的结构与生物活性的关系[J].生物学杂志,2005,2(1):4-7.
[5]谢鹏,张敏红.黄酮类化合物抑菌作用的研究进展[J].中国动物保健,2004,12:35-37.
[6]VISWANATHAN P,SRIRAM V,YOGEESWARAN G.Sensitive spectrophotometric assay for 3-hydroxy-substituted flavonoids,based on their binding with molybdenum,antimony,or bismuth[J].J.Agric.Food Chem.,2000,48:2802-2806.
[7]HYNES M J,MAIRTIN T,COINCEANAINN O.The kinetics and mechanisms of reactions of iron (Ⅲ) with caffeic acid,chlorogenic acid,sinapic acid,ferulic acid and naringin[J].J.Inorg.Biochem.,2004,98:1457-1464.
[8]EL-NYAAN U,HERLINGER E,JAMESON R G,et al.Anaerobic oxidation of dopamine by iron (Ⅲ)[J].J.Chem.Soc.Dalton Trans.,1997,2813-2818.
[9]EL-NYAAN U,JAMESON R F,LINERT W,et al.A kinetic study of the reaction between noradrenatine and iron(Ⅲ):an example of parallel inner-and outer-sphere electron transfer[J].J.Chem.Soc.Dalton Trans.,1998,1315-1319.
[10]LERMAN L S.Structural considerations in the interaction of DNA and acridines[J].J.Mol.Biol.,1961,3:18-30.
[11]STEINER R T,WE1NRYB L.Excited States of Protein and Nucleic Acid[M].Plenum Press,New York,1971,40.
[12]LAKOWICZ J R.Principles of Fluorescence Spectroscopy[M].Second ed.New York:Plenum Press,1999,237-259.
[13]LAKOWICA J R,WEBER G.Quenching of fluorescence by oxygen.Probe for structural fluctuations in macromolecules[J].Biochem.,1973,12:4161-4170.
[14]WARE W R.Oxygen quenching of fluorescence in solution:an experimental study of the diffusion process[J].J.Phys.Chem.,1962,66:455-458.
[15]LAKOWICZ J R.Principles of Fluorescence Spectroscopy[M].Plenum Press,New York,1983,257.
[16]SILVA D,CORTEZ C M,LOURO S R W.Chlorpromazine interactions to sera albumins a study by the quenching of fluorescence[J].Spectrochim.Acta Part A,2004,60:1215-1223.
[17]BI S Y,SONG D Q,TIAN Y,et al.Molecular spectroscopic study on the interaction of tetracyclines with serum albumins[J].Spectrochim.Acta Part A,2005,61:629-635.
[18]RABINDRANATH B,BIJAYA K S,KALYAN S G,et aI.Studies on the interaction of isoxazolcurcumin with calf thymus DNA[J].Int.J.Biol.Macromol.,2008,42:14-21.
[19]ROSS D P,SUBRAMANIAN S.Thermodynamic of protein association reactions:forces contributing to stability[J].Biochem.,1981,20:3096-3102.
[20]HAQ I.Thermodynamics of drug-DNA interactions[J].Arch.Biochem.Biophys.,2002,403:1-15.
[21]杨曼曼,杨频,席小莉.荧光染料探针与蛋白质结合的研究[J].科学通报,1997,42(12):1276-1279.
[22]郭尧君.荧光实验技术及其在分子生物学中的应用[M].北京:科学出版社,1983.
[23]CLEGG R M.Fluorescence resonance energy transfer[J].Curr.Opin.Biotechnol.,1995,6:103-110.
[24]DOS REMEDIOS C G,MOENS P D.Fluorescence resonance energy transfer spectroscopy is a reliable "ruler" for measuring structural changes in proteins.Dispelling the problem of the unknown orientation factor[J].J.Struct.Biol.,1995,115:175-185.
[25]SKLAR L A,HUDSON B S,SIMONI R D.Conjugated polyene fatty acids as fluorescent probes:binding bovine serum albumin[J].Biochem.,1977,16:5100-5108.
[26]LAKOWlCZ J R,WEBER G.Principles of Fluorescence Spectroscopy[M].Plenum Press,New York,1983,305-337.
[27]EPPS D E,RAUB T J,CAIOLFA V,et al.Determination of the affinity of drugs toward serum albumin by measurement of the quenching of the intrinsic tryptophan fluorescence of the protein[J].J.Pharm.Pharmacol.,1999,51:41-48.
[28]CYRIL L,EARL J K,SPERRY W M.Biochemists Handbook[M].London:E & FN Epon Led.Press,1961,83
[1]唐文涛.辨证治疗病毒性心肌炎85例[J].实用中医药杂志,1998,14(10):8-9.
[2]江苏新医学院.中药大辞典(上)[M].上海:上海人民出版社,1977.
[3]李正民.牛蒡子引起过敏反应报告[J].实用中医内科杂志,1994,8:37.
[4]赵华,王劲,杨松松.药用植物牛蒡化学成分和药理活性研究述要[J].辽宁中医学院学报,2005,7(2):3-4.
[5]郑国灿.牛蒡子苷对胰腺癌细胞抑制作用及其作用机理的实验研究[J].时珍国医国药,2008,10:2384-2386.
[6]HUANGD M,GUH J H,CHUEH S C,et al.Modulation of anti-adhesion molecule MUC-1 is associated with arctiin-induced growth inhibition in PC-3 cells[J].Prostate,2004,59(3):260-267.
[7]陈铁宏,黄迪.牛蒡子对Epstein-Barr病毒抗表达的抑制作用[J].中华实验和临床病毒学杂志,1994,8(4):323-326.
[8]杨子峰,刘妮,黄碧松等.牛蒡子甙元体内抗甲1型流感病毒作用的研究[J].中药材,2005,28(11):1012-1014.
[9]LIU S,Li Y,LI S,et al.An experimental research into the anti-aging effects of Radix Arctii Lappae [J].J.Tradit.Chin.Med.,2005,25(4):296-299.
[10]KARDOSOVA A,MACHOVA E.Antioxidant activity of medicinal plant polysaccharides[J].Fitoterapia,2006,77(5):367-373.
[11]CHO J Y,KIM A R,YOO E S,et al.Immunomodulatory effect of arctigenin oxide production and lymphocyte proliferation[J].J.Pharm.Pharmacol.,1995,51(11):1267-1273.
[12]CHO M K,JANG Y P,KIM Y C,et al.Arctigenin,a phenylpropanoid dibenzylbutyrolactone lignan,inhibits MAP kinases and AP-1 activation via potent MKK inhibition:the role in TNF-a inhibition[J].Int Immunopharmacol,2004,4(10-11):1419-1429.
[13]王淑静,魏永巨.牛蒡子中牛蒡苷含量的荧光法测定[A].国家自然科学基金委员会化学科学部.第二届全国生命分析化学学术报告与研讨会论文集[C].北京:,2008:19
[14]李慧义,罗淑荣.牛蒡子中木脂素的薄层扫描测定[J].色谱,1995,13(4):277-278.
[15]中国药典一部[M],北京:人民卫生出版社,2005:48.
[16]杨铭.以DNA为靶的小分子药物研究中的分子识别[J].北京医科大学学报,1998,30(2):97-100.
[17]宋凤瑞,闫存玉,刘宁等.核酸靶分子与某些中药成分的非共价作用研究[J].分析化学,2005.33:1743-1746.
[18]MARMUR J.A predure for the isolation of deoxyribonucleic acid from microorganism[J].J.Mol.Biol.,1961,3:208-218.
[19]MAHLER H R,KLINE B,MEHROTRA B D.Some observations on the hpochromism of DNA [J].J.Mol.Biol.,1964,9:801-811.
[20]PYLE A M,REHMANN J P,MESHOYRER R,et al.Mixed-ligand complexes of ruthenium(Ⅱ):factors governing binding to DNA[J].J.Am.Chem.Soc.,1989,111:3051-3058.
[21]LERMAN L S.Structural considerations in the interaction of DNA and acridines[J].J.Mol.Biol.,1961,3:18-30.
[22]STEINER R T,WEINRYB L.Excited States of Protein and Nucleic Acid[M].Plenum Press, New York,1971,40.
[23]LAKOWICZ J R.Principles of Fluorescence Spectroscopy[M].Second ed.New York:Plenum Press,1999,237-259.
[24]LAKOWlCA J R,WEBER G.Quenching of fluorescence by oxygen.Probe for structural fluctuations in macromolecules[J].Biochem.,1973,12:4161-4170.
[25]WARE W R.Oxygenquenchixg of fluorescence in solution:an experimental study of thediffusion process[J].J.Phys.Chem.,1962,66(3):455-458.
[26]ROSS P D,SUBRAMANIAN S.Thermodynamics of protein association reactions:forces contributing to stability[J].Biochem.,1981,20:3096-3102.
[27]JIANG C Q,HE J X,WANG J S.Studies on the interaction mode between polymyxin B sulfate and DNA with EB as a fluorescence probe[J].Spectroscopy and Spectral Analysis,2002,22(1):103-106.
[28]Fuller W,Warning M J.Ber.A molecular model for the interaction of ethidium bromide with deoxyribonucleic acid[J].Bet.Bunsen-Ges.Phys.Chem.1964,68,805-808.
[29]LEPECQ J B,PAOLETLI C.A new fluorometric method for RNA and DNA determination.Anal.Biochem.,1966,17:100-107.
[30]REINHARDT C G,KRUGH T R.A comparative study of ethidium bro-mide complexes with dinucleotides and DNA:direct evidence for intercalation and nucleic acid sequence preferences[J].Biochem.,1978,17(23):4845-4853.
[31]KUMAR C V,TURNER R S.Groove binding ofa styrylcyanine dye to the DNA double helix:the salt effect[J].J.Photochem.Photobio.A:Chem.,1993,74:231-238.
[32]RAIAMANICKAM V,MOOKANDI K,VENKATESAN S,et al.Interaction of DNA with [Cr(Schii base)(H_2O)_2]ClO_4[J].Biochim.Biophys.Acta.,2000,1475:157-162.
[33]李来生,黄志兵,王宇晓等.荧光光谱法研究对-二甲氨甲基-杯[8]芳烃与DNA相互作用[J].光谱学与光谱分析,2005,25(7):1088-1092.
[34]PASTERNACK R F,BRIGANDI R A,ABRAMS M J.Interactions of porphyrins and metalloporphyrins with single-stranded poly(dA)[J].Inorg.Chem.,1990,29:4483-4486.
[35]梅文杰,刘杰,李安兴等.钌卟啉[Ru(bpy)_2(MPyTPP)Cl]~+与DNA作用的研究[J].高等学校化学学报,2002,23(11):2049-2051.
[36]PRATVIEL G,BERNADOU J,MEUNIER B.Carbon-hydrogen bonds of DNA sugar units as targets for chemical nucleases and drugs [J]. Angew. Chem. Int. Ed. Engl., 1995, 34: 746-769.
[37] KUMAR C V, TURNER R S, ASUNCION E H. Groove binding of a styrylcyanine dye to the DNA double helix: The salt effect [J]. J. Photochem. Photobiol. A: Chem., 1993, 74: 231-238.
[1]马绍宾,胡志浩.小檗科鬼臼亚科的地理分布与系统发育[J].云南植物研究,1997,19(1):48-56.
[2]KING L S,SULLIVAN M.The similarity of the effect of podophyllin and colchicine and their use in the treatment of condylomata acuminata[J].Science,1946,104:244-245.
[2]杨显志,邵华,张玲琪等.鬼臼毒素资源研究现状[J].中草药,2001,32(11):1042-1044.
[3]潘勤.鬼臼毒素类环木脂素的甲醚衍生物[J].国外医药-植物药分册,1996,11(2):73-74.
[4]LEE K H,SCOTT A B,MASAMI M,et al.Antitumor agents.111 New 4-hydroxylated and 4-halogenated anilino derivatives of 4'-demet hylepipodophyllotoxin as potent inhibitors of human DNA topoisomerase Ⅱ[J].J.Med.Chem.,1990,33(5):1364-1368.
[5]朱晓亮,曾抗,陈飞龙.紫外分光光度法测定鬼臼毒素壳聚糖膜剂中鬼臼毒素的含量[J].中国现代医学杂志,2005,15(5):769-771.
[6]陈燕,德吉,刘倩伶等.桃儿七果实中鬼臼毒素的鉴别和含量测定[J].华西药学杂志,2009,24(1):93-94.
[7]张丽艳,万明香,何顺志等.HPLC法测定贵州产八角莲属药材中鬼臼毒素的含量[J].药物分析杂志,2009.29:202-204.
[8]张杰,周春山,向大雄等.高效液相色谱法测定小八角莲中鬼臼毒素含量[J].湖南中医杂志,2007,23(4):200-201.
[9]马守栋,孟庆礼.荧光分光光度法测定鬼臼毒素的含量[J].中国医院药学杂志,1998,18,(4):162-163.
[10]GLINSKI M B,FREED J C,DURST T J.Novel stereoselective synthesis of(-)-enterolactone employing chiral unsaturated lactam[J].Org.Chem.,1987,52:2749-2753.
[11]王庆文,杨玉桓,高鸿宾.有机化学中的氢键问题[M].天津:天津大学出版社,1993:10-12.
[12]徐国钧.中国药材学[M].北京:中国医药科技出版社,1993:24.
[13]BAI H,LI W,KOIKE B K,et al.A Novel Biflavonoid from Roots of Glycyrrhiza uralensis Cultivated in China[J].Chemical and Pharmaceutical Bulletin,2003,51(9):1095-1097.
[14]张雪辉,赵元芬,陈建民.甘草中总黄酮的含量测定[J].中国中药杂志,2001,26(11):746-749.
[15]何三民,石森林.HPLC法测定甘草中甘草素、异甘草素、甘草苷的含量[J].中草药,2003,34(7):618-619.
[16]丁选胜,戴德哉.甘草苷对四氯化碳肝脏毒性的保护作用[J].中草药,2003,34(12):1122-1123.
[17]赵志宇,王卫星,郭洪祝等.甘草苷对慢性应激抑郁模型大鼠的抗抑郁作用.中国临床康复,2006,10(27):69-72.
[18]王小强.薄层光密度法测定甘草中甘草甙的含量[J].药物分析杂志,1990,10(6):351-352.
[19]段天璇,于密密,刘春生等.HPLC法同时测定甘草指纹图谱暨甘草苷、甘草酸含量[J].中成药,2006,28(2):161-165.
[20]靳凤云,贺祝英,赵杨等.HPLC测定华盖散传统汤剂与颗粒汤剂中盐酸麻黄碱、苦杏仁苷、甘草酸、甘草苷的含量[J].中成药,2008,30(1):80-87.
[21]犹卫,郑志祥,高作宁.甘草苷在悬汞电极上的电化学行为及方法研究[J].分析试验室.2008,27(7):5-8.
[22]陈亚东,曹秀兰.高山红景天对小鼠耐缺氧、抗疲劳及耐低温作用的影响[J].中国中医药科技,2002,9(3):157-158.
[23]刘志伟,吴谋成,陈平.菱叶红景天对小鼠学习记忆影响及耐缺氧实验[J].营养学报,2003,25(1):101-104.
[24]骆传环,高月,王作华等.红景天多糖的抗放实验的研究[J].中华放射医学与防护杂志,1999,14(5):340-341.
[25]中国医学科学院.中国协和医科大学医学信息研究所.医学主题词表[M].北京:中国计量出版社,2002.
[26]赵文,蒋东升,边庆荣等.蔷薇红景天提取液对小鼠免疫功能的影响[J].卫生毒理学杂志,2000,14(3):159.
[27]余国祥,蔡鹰.红景天生脉口服液抗实验性心律失常的作用[J].云南中医中药杂志,2003,24(4):37-38.
[28]毛静远,王化良,赵如菊等.红景天胶囊治疗冠心病心绞痛临床研究[J].中国医药学报,2002,17(8):480-483.
[29]李君,陈志,李建民等.红景天的研究进展[J].云南农业大学学报,2007,22:61-64.
[30]张早华,储戟农,林文翰等.高效液相色谱法测定红景天注射液中红景天苷的含量[J].中国中医药信息杂志,2005,12(11):51-52.
[31]杨晓艳,芦启琴,张晓峰.HPLC法测定红景天根皮中红景天苷的含量.分析试验室,2008,27:305-306.
[32]安伟建,陈冠,王文彤等.HPLC法测定不同产地红景天属植物中红景天苷[J].中草药,2007,38(7):1096-1097.
[33]孟健,王淑芬,韩飞等.SPE-HPLC测定注射用红景天(冻干)中红景天苷和酪醇的含量[J].中国中药杂志,2006,31(2):119-121.
[34]陈志英,陈玉婷,王丹红.HPLC测定不同品种红景天中红景天苷的含量[J].2006,31(11):939-941.
[2]CARTER D,HO J X.Structure and ligand binding properties of human serum albumin[J].Adv Protein Chem.,1994,45:153-203.
[3]BROWN J R,SHOCKLEY P.Lipid-Protein Interactions,vol.1,Wiley,New York,1982.
[4]CARTER D C,HE X M,MUNSON S H,et al.Three-dimensional structure of human serum albumin[J].Science,1989,244(4909):1195-1198.
[5]Carter D C,He X M.Structure of human serum albumin[J].Science,1990,249(4966):302-303.
[6]HE X M,CARTER D C.Atomic structure and chemistry of human serum albumin[J].Nature,1992,358:209-215.
[7]KRAGH-HANSEN U.Molecular aspects of ligand binding to serum albumin[J].Pharmcol.Rev.,1981,33:17-53.
[8]PETERS T.Serum albumin.Adv.Protein Chem.,1985,37:161-245.
[9]SUDLOW G,BIRKETT D J,WADE D N.The Characterization of Two Special Drug Binding Sites on Human Serum Albumin[J].Mol.Pharmacol.,1975,11(6):824-832.
[10]FEHSKE K J,M(U|¨)LLER W E,WOLLERT U.The location of drug binding sites in human serum albumin[J].Biochem.Pharmacol.,1981,30:687-592.
[11]HE X M,CARTER D C.Atomic structure and chemistry of human serum albumin[J].Nature,1992,358(2):209-215.
[12]SJ(O|¨)HOLM I,EKMAN B,KOBER A,et al.Binding of drugs to human serum albumins-Ⅸ.The specificity of three binding sites as studied with albumin immobilised in microparticles[J].Mol.Pharmacol.,1979,16(3):767-777.
[13]FEHSKE K J,SCHL(A|¨)FER U,WOLLERT U,et al.Characterization of an important drug binding area on human serum albumin including the high-affinity binding sites of warfarin and azapropazone [J].Mol.Pharmacol.,1982,21(2):387-393.
[14]SUDLOW G,BIRKETT D J,WADE D N.Further characterization of specific drug binding sites on human serum albumin[J],Mol.Pharmacol.,1976,12,1052-1061.
[15]徐岩,沈含熙,黄汉国.利用标记药物布洛芬及保泰松研究萘啶酸与血清白蛋白的结合作[J].高等化学学报,1996,17(12):1855-1858.
[16]YAMASAKI K,MARUYAMA T,KRAGH-HANSEN U,ET AL.Characterization of site I on human serum albumin:concept about the structure of a drug binding site[J].Biochim.Biophys.Acta,1996,1295(2):147-157.
[17]BARONI S,MATTU M,VANNINI A,et al.Effect of ibuprofen and warfarin on the allosteric properties of heine-human serum albumin.A spectroscopic Study[J].Eur.J.Biochem.,2001,268:6214-6220.
[18]WILTING J,van der GIESEN W F,JANSSEN L H,et al.The effect of albumin conformation on the binding of warfarin to human serum albumin.The dependence of the binding of warfarin to human serum albumin on the hydrogen,calcium,and chloride ion concentrations as studied by circular dichroism,fluorescence,and equilibrium dialysis[J].J.Biol.Chem.,1980,255:3032-3037.
[19]PETITPAS I,BHATTACHARYA A A,TWINE S M,et al.Crystal structure analysis of warfarin binding to human serum albumin[J].J.Biol.Chem.,2001,276(25):22804-22809.
[20]KRAGH-HANSEN U,CHUANG V Y G,OTAGIRI M.Practical aspects of the ligand-binding and enzymatic properties of human serum albumin[J].Biological & Pharmaceutical Bulletin,2002,25(6):695-704.
[21]MANDULA H,PAREPALLY J M R,FENG R,ET AL.Role of site-specic binding to plasma albumin in drug availability to brain[J].J.Pharmacol.Exp.Yher.,2006,317(2):667-675.
[22]NI Y N,SU S J,KOKOT S.Spectrofluorimetric studies on the binding of salicylic acid to bovine serum albumin using warfarin and ibuprofen as site markers with the aid of parallel factor analysis[J].Anal.Chim.Acta,2006,580(2):206-215.
[23]DUFOUR C,DANGLES O.Flavonoid-serum albumin complexation:determination of binding constants and binding sites by fluorescence spectroscopy[J].Biochim.Biophys.Acta,2005,1721(1):164-173.
[24]BOS O J M,REMIJN J P M,FISCHER M J E,et al.Location and characterization of the warfarin binding site of human serum albumin,A comparative study of two large fragments[J].Biochem.Pharmacol.,1988,37:3905-3909.
[25]KOSA T,MARUYAMA T,OTAGIRI M.Species differences of serum albumin:I.Drug binding sites[J].Pharm.Res.,1997,14(11):1607-1612.
[26]WATSON J D,CRICK F H C.Molecular structure of nucleic acids-a structure for deoxyribose nucleic acid[J].Nature,1953,171:737-738.
[27]FRATINI A V,KOPKA M L,DREW H,et al.J.Reversible bending and helix geometry in a B-DNA dodecamer:CGCGAATTBrCGCG[J].Biol.Chem.,1982,257:14686-14707.
[28]NELSON H C,FINCH J T,LUISI B F,et al.The Structure of an Oligo(Da).Oligo(dT) tract and its biological implications[J].Nature,1987,330:221-226.
[29]PULLMAN B.Electrostatics of Polymorphic DNA[J].J.Biomol.Struct.Dyn.,1983,1(3):773-794.
[30]KOPKA M L,YOON C,GOODSELL D,et al.The molecular origin of DNA-drug specificity in netropsin and distamycin[J].Proc.Natl.Acad.Sci.,1985,82(5):1376-1378.
[31]LEE K H,BENSON D R,KUCZERA K.Transitions from α to π helix observed inmolecular dynamics simulations of synthetic peptides[J].Biochem.,2000,39(45):13737-13747.
[32]LAMPELA O,JUFFERA H,RAUK A.Conformational analysis of glutathione in aqueous solution with molecular dynamics[J].J.Phy.Chem.A,2003,107(43):9208-9220.
[33]VAMAI P,LAVERY R.Base flipping in DNA:pathways and energetics studied with molecular dynamic simulations[J].J.Am.Chem.Soc.,2002,124(25):7272-7273.
[34]JEREMY B JOHN T,LUBERT S.Biochemistry[M].5th ed.New York:W.H.Freeman and Company,2002 Chap.1.The Molecular Design of Life.In:NCBI online bookshelf.
[35]张荣.生化模型分子及生物小分子水溶液弱相互作用研究[C].博士学位论文杭州浙江大学,2006.
[36]周新,汪子明,邹明强等.亲和毛细管电泳法测定3种黄酮类化合物与人血清白蛋白的结合常数[J].吉林大学学报,2005,43(5):669-672.
[37]郭明,严建伟,俞庆森等.加替沙星与牛血清白蛋白的结合反应研究[J].物理化学学报,2004,20(2):202-206.
[38]邬春华,吕元琦,袁倬斌.大黄酸与牛血清白蛋白相互作用的电化学研究[J].分析测试学报,2004,23(3):73-75.
[39]龙德武,刘传银,赵鸿雁等.铜Ⅱ-三氮唑偶氮络合物与脱氧核糖核酸相互作用的电化学行为[J].分析化学,2002,30(10):1250-1253.
[40]李天剑,沈含熙,罗云敬.乙基紫标记分光光度法测定脱氧核糖核酸[J].分析化学,1998,26(11):1372-1375.
[41]宋功武,方光荣,冯健.甲基紫核酸分子相互作用的紫外-可见光谱及其分析应用[J].分析化学,2000,28(1):128-132.
[42]俞英,程晓伟.牛血清白蛋白与酸性品红的结合反应[J].分析化学,2000,28(5):583-586.
[43]王春,吴秋华,王志等.槲皮素与牛血清白蛋白相互作用的研究[J].光谱学与光谱分析,2006,26(9):1672-1675.
[44]KELLY S M,JESS T J,PRICE N C.How to study proteins by circular dichroism[J].Biochimica et Biophysica Acta,2005,1751(2):119-139.
[45]梁毅著.结构生物化学[M].北京:科学出版社,2005,307.
[46]GERBANOWSKI A,MALABAT C,RABILLER C,et al.Grafting of aliphatic and aromatic probes on rapeseed 2S and 12S proteins:influence on their structural and physicochemical properties [J].J.Agric.Food Chem.,1999,47:5218-5226.
[47]杨频,高飞编著.生物无机化学原理[M].北京:科学出版社,2002.
[48]沈星灿,梁宏,何锡文等.圆二色光谱分析蛋白质构象的方法及研究进展[J].分析化学,2004,4(3):388-394.
[49]于海英,程秀民,王晓坤.圆二色光谱及其在药物研究方面的应用[J].光谱实验室,2007,24(5):877-885.
[50]Provencher S W,Glockner J.Estimation of globular protein secondary structure from circular dichroism[J].Biochem.,1981,20:33-37.
[51]CHIGNELL C F.Optical studies of drug-protein complexes:Ⅲ.interaction of flufenarnic acid and other N-Arylanthranilates with serum albumin[J].Mol.Pharmacol.,1969,5:455-462.
[52]Chignell C.F.Optical Studies of drug-protein complexes:Ⅱ.interaction ofphenylbutazone and its analogues with human serum albumin[J].Mol.Pharmacol.,1969,5:244-252.
[53]HWANGBO H J,YUN B H,CHA J S,et al.Enantioselective binding of S-and R-ofloxacin to V arious synthetic polynucleotides[J].European Journal of Pharmaceutical Sciences,2003,18(2):197-203.
[54]KANDAGAL P B,SEETHARAMAPPA J,ASHOKA S,et al.Study of the interaction between doxepin hydrochloride and bovine serum albumin by spectroscopic techniques[J].Int.J.Biol.Macromol,2006,39(4-5):234-239.
[55]王建林,付连春,周实武等.维生素B_6与人血清白蛋白的相互作用[J].光谱学与光谱分析,2005,25(6):912-915.
[56]RODGER A,BLAGBROUGH I S,ADLAM G,et al.DNA binding of a spermine derivative:spectroscopic study of anthracene-9-carbonyl-N1-spermine with poly[d(G-C).d(G-C)]and poly[d (A-T).d(A-T)][J].Biopolymers,1994,34:1583-1593.
[57]王云普,萧耀南,王荣民.水溶性高分子金属钴卟啉络合物的合成及其与DNA作用的研究[J].西北师范大学学报(自然科学版),2000,369(1):40-44.
[58]THEOPHANIDES T.Fourier Transform Infrared Spectra of Calf Thymus DNA and its Reactions with the Anticancer Drug Cisplatin[J].Appl.Spectrosc.,1981,35:461-465.
[59]WILSON W D.Intercalation Chemistry[M].Academic Press,1982,445-501.
[60]王树玲,于俊生.表面增强拉曼光谱研究小檗碱与DNA的相互作用[J].高等学校化学学报.2002,23(9):1676-1679.
[61]GAMBARI R,FERIOTTO G,RUTIGLIANO C,et al.Biospecific Interaction Analysis(BIA) of Low-Molecular Weight DNA-Binding Drugs[J].J.Pharmacol.Exp.Ther.,2000 294:370-377.
[62]MINUNNI M,TOMBELLI S,MASCINI M,et al.An optical DNA-based biosensor for the analysis of bioactive constituents with application in drug and herbal drug screening[J].Talanta,2005,65:578-585.
[63]AHMAD A,RAMAKRISHNAN A,MCLEAN M A,et al.Use of surface plasmon resonance biosensor technology as a possible alternative to detect differences in binding of enantiomeric drug compounds to immobilized albumins.Biosensors and Bioelectronics,2003,18:399-404.
[64]DANELIAN E,KARLEN A,KARLSSON R,et al.SPR biosensor studies of the direct interaction between 27 drugs and a liposome surface:Correlation with fraction absorbed in humans.J.Med.Chem.,2000,43:2083-2086.
[65]NEDELKOV D,NELSON R W.Delineating protein-protein interations via biomolecular interaction analysis-mass spectrometry[J].J.Mol.Recognit.,2003,16(1):9-14.
[66]KIKUCHI J,FURUKAWA Y,HAYASHI N.Identification of novel P53-binding proteins by biomolecular interaction analysis combined with tandem mass spectrometry[J].Mol.Biotechnol.,2003,23(3):203-212.
[67]潘祖,刘义庆,刘小玲.药物荧光分析法研究进展[J].武汉大学学报(自然科学版),2000,46(16):674-680.
[68]LAKOWICA J R,WEBER G.Quenching of fluorescence by oxygen.Probe for structural fluctuations in macromolecules[J].Biochem.,1973,12:4161-4170.
[69]EFTINK M R.In Biophysical and Biochemical Aspects of Fluorescence Spectroscopy(ed.Dewey T G,)[M].Plenum Press,New York,1991,1-41.
[70]WARE W R.Oxygen quenching of fluorescence in solution:an experimental study of the diffusion process[J].J.Phys.Chem.,1962,66:455-458.
[71]LAKOWICZ J R.Principles of Fluorescence Spectroscopy,second ed.[M].New York:Plenum Press,1999,237-259.
[72]LAKOWICZ J.R.Principles of Fluorescence Spectroscopy,third ed.[M].Springer,2006,278-282.
[73]陈晓波,李崧.铜、钠和钼离子与牛血清白蛋白作用的荧光光谱研究[J].光谱学与光谱分析,2006,26(2):309-312.
[74]TWINE S M,GORE M G,MORTON P,et al.Mechanism of binding of warfarin enantiomers to recombinant domains of human albumin[J].Arch.Biochem.Biophys.,2003,414(1):83-90.
[75]易长海,颜承农,上官云凤等.苯噻啶与蛋白质作用特征的热力学研究[J].华中师范大学学报,自然科学版2002,32(3):317-319.
[76]玄光善,吴效楠,李玉平.荧光光谱法研究乙酰水杨酸和牛血清蛋白的相互作用[J].光谱实验室,2005,22(4):861-864.
[77]王彦卿,张红梅,张朝平.荧光光谱法研究茶碱与血红蛋白的相互作用[J].化学试剂,2006,28(3):156-158.
[78]盛良全,童红武,张淑萍等.烟碱与人血清蛋白相互作用的光谱研究[J].分析化学,2006,34:S203-S206.
[79]刘永明,王进军.脱镁叶绿酸α-甲酯的合成及对牛血清白蛋白的结合作用[J].光谱学与光谱学分析,2005,25(4):747-750.
[80]LEHRER S S,Solute perturbation of protein fluorescence[J].Biochem.,1971,10:3254-3263.
[81]TIMASEFF S N.Thermodynamics of protein interactions[M].In:Proteins of biological fluids (Ed.Peeters H),Pergamon Press,Oxford,1972,511-519.
[82]CARTER D C,CHANG B,HO J X,et al.Preliminary Crystallographic Studies of Four Crystals Forms of Serum Albumin[J].Eur.J.Biochem.,1994,226:1049-1052.
[83]刘家琴.中药活性组分与蛋白质相互作用研究[D].兰州:兰州大学,2004.
[84]ASHOKA S,SEETHARAMAPPA J,KANDAGAL P B,et al.Investigation of the interaction between trazodone hydrochloride and bovine serum albumin[J].Journal of Luminescence,2006,121:179-186.
[85]SHAIKH S M T,SEETHARAMAPPA J,ASHOKA S,et al.Fluorescent homodimer styrylcyanines:synthesis and spectral-luminescent studies in nucleic acids and protein complexes[J].Dyes and Pigments 2007,73:211-216.
[86]WU P,BRAND L.Review-Resonance energy transfer[J].Meth Appl Biochem,1994,218:1-13.
[87]F(O|¨)RSTER T.Intramolecular energy migration and fluorescence[J].Ann.Phys.,1948,2:5-75.
[88]F(O|¨)RSTER T,SINANOGLU O.Modern Quantum Chemisby[M].New York:Academic Press,1966.
[89]STRYER L,HAUGLAND R.Energy transfer:a spectroscopic ruler[M].Proc.Natl.Acad.Sci.USA,1967,58:719-726.
[90]陈国珍,黄贤智,许金钩等.荧光分析法(第二版)[M].科学出版社,北京,1990,123.
[91]马贵斌,高尺,任斌知等.荧光法研究药物分子与人血清白蛋白的结合作用[J].化学学报,1995,53(12):1193-1197.
[92]HEIM R,PRASHER D C,TSIEN R Y.Wavelength mutations and posttranslationalauxidation of green fluorescent protein[J].Proc.Natl.Acad.Sci.USA,1994,91:12501-12504.
[93]SUZUKIDA M,LE H P,SHAHID F,et al.Resonance energy transfer between cysteine-34 and tryptophan-214 in human serum albumin.Distance measurements as a function of pH[J].Biochem.,1983,22:2415-2420.
[94]汪海林,邹汉法,张玉奎.微透析-液相色谱研究药物与蛋白竞争结合[J].中国科学辑,1998,28(1):71-77.
[95]KRAAK J C,BUSCH S,POPPE H.Study of protein-drug binding using capillary zone electrophoresis[J].J.Chromatogr.A,1992,608(1-2):257-264.
[96]SCATCHARD G,SCHEIBERG I H,ARMSTRONG S H.Physical chemistry of protein solutions Ⅳ:the combination of human serum albumin with Chloride Ion[J].J.Am.Chem.Soc.,1950,72(1):535-541.
[97]SCATCHARD G.The attractions of protein for small molecules and ions[J].Ann.N.Y.Acad.Sci.,1949,51(4):660-672.
[98]YANG P,YANG M M,YANG B S.The fluorescence enhancement and the action between donor and acceptor[J].Chin.J.Chem.,1996,14(2):109-113.
[99]杨曼曼,杨频,席小莉.荧光染料探针与蛋白质结合的研究[J].科学通报,1997,42(12):1276-1279.
[100]JAMES D M,PETER H.Theoretical aspects of DNA-Protein interactions:Co-operative and Non Co-operative binding of large ligands to a one-demensional homogeneous lattice[J].J.Mol.Biol.,1974,86:469-489.
[101]CONGDON R W,MUTH G W,SPLITTGERBER A G.The binding interaction of coomassie blue with proteins[J].Anal.Biochem.,1993,213:407-413.
[102]KLOTZ I M,HUNSTONE D L.Properties of graphical representations of multiple classes of binding sites[J].Biochem.,1971,10:3065-3069.
[103]McGHEE J D,von HIPPEL P H.Theoretical aspects of DNA-Protein interactions:Co-operative and Non Co-o-perative binding of large ligands to a one-demensional homogeneous lattice[J].J.Mol.Biol.,1974,86:469-489.
[104]ReLNHARDT C G,KRUGH T R.A comparative study of ethdium bromide complexe with dinucleotide and DNA:direct evidence for intercalation and nucldc acid sequence preferences[J].Biochem.,1978,17:4845-4854.
[105]LEPECQ J B,PAOLETTI C.A fluorescent complex between ethidium bromide and nucleic acids.Physical-chemical characterization[J].J.Mol.Biol.,1967,27(1):87-106.
[106]YI P G,YU Q S,SHANG Z C,et al.The reaction mechanism between ofloxacin and bovine serum albumin[J].Acta Pharm.Sin.,2000,35:774-777.
[107]ALAIN M,MICHEL B,MICHEL A.How to illustrate ligand-protein binding in a class experiment:An elementary fluorescent assay[J].J.Chem.Edu.,1986,63(4):365-366.
[108]张勇,张贵珠,王月梅等.光谱法研究丝裂霉素、血清白蛋白以及金属离子间的相互作用[J].分析科学学报,2000,16(6):44-49.
[109]ZHAO H W,GE M,ZHANG Z X,et al.Spectroscopic studies on the interaction between riboflavin and albumins[J].Spectrochim.Acta,Part A,2006,65(3-4):811-817.
[110]FENG X Z,LIN Z,YANG L J,et al.Piezoelectric quartz crystal biosensors(Review)[J].Talanta,1998,47:1223-1236.
[111]BARIK A,PRIYADARSINI K I,MOHAN H.Photophysical studies on binding of curcumin to bovine serum albumin[J].Photochem.Photobiol.,2003,77:597-603.
[112]Bi S Y,Zhang H Q,Qiao C Y,et al.Studies of interaction of emodin and DNA in the presence of ethidium bromide by spectroscopic method[J].Spectrochim.Acta.Part A,2008,69:123-129.
[113]JONES M N.Surfactant interactions with biomembranes and proteins[J].Chem.Soc.Rev.,1992,21:127-136.
[114]HALFMAN C J,NISHIDA T.Measuring the binding of small molecules to protein from binding-induced alterations of physical-chemical properties[J].Biochem.,1972,11(18):3493-3498.
[115]JONES M N,CHAPMAN D.Micelles,Monolayers and Biomembranes[M].Wiley-Riss,New York,1995,ch.6,143-179.
[116]ROSENTHANL H R.Graphic method for the determination and presentation of binding parameters in a complex system[J].Anal.Biochem.,1967,20,525-532.
[117]SUR S S,RABBANI L D,LIBMANL L,ET AL.Fluorescence studies of native and modified neurophysms.Efects of piptides and pH[J].Biochem.,1979,18(6):1026-1035.
[118]LAKOWICZ J R.Principles of Fluorescence Spectroscopy[M],2nd Ed.,Kluwer Academic/Plenum Publishers,New York,1999.
[119]LERMAN L S.Structural considerations in the interaction of DNA and acridines[J].J.Mol.Biol.,1961,3:18-30.
[120]STEINER R T,WEINRYB L.Excited States of Protein and Nucleic Acid[M].Plenum Press,New York,1971,40.
[121]徐岩,黄汉国,沈含熙.喹诺酮类药物对人血清白蛋白的荧光猝灭研究[J].分析化学,1998,12:1494-1497.
[122]徐岩,沈含熙,黄汉国.利用标记药物布洛芬及保泰松研究萘啶酸与血清白蛋白的结合作用[J].高等化学学报,1996,17(12):1855-1858.
[123]张霞,倪永年.荧光光谱法研究盐酸曲唑酮与BSA的相互作用[C].南昌大学学报增刊(第九届中国化学会分析化学年会暨地九届全国原子光谱学术会议论文集),2006,30:337-338.
[124]TIAN J N,LIU J Q,XIE J Q,et.al.Binding of wogonin to human serum albumin.A common binding site ofwogonin in Subdomain ⅡA[J].J.Photochem.Photoboil.B:Biology,2004,74:39-45.
[125]DUFOUR C,DANGLES O.Flavonoid-serum albumin complexation:determination of binding constants and binding sites by flurescence specteoscopy[J].Biochim.Biophys.Acta.,2005,1721(1):164-173.
[126]MORENO F,GONZALEZ-JIMENEZ J.Binding of the Promen fluorescent probe to human serum albumin:a fluorescence spectroscopic study[J].Chem.Biol.Interact.,1999,121(2):237-252.
[127]SOLTES L,MACH M.Estimation of drug-protein binding parameters on assuming the validity of thermodynamic equilibrium[J].J.Chromatogr.B.,2002,768:113-119.
[128]张保林,王文清,白凤莲.蒽醌及黄酮类化合物与人血清白蛋白的结合反应研究[J].高等学校化学学报,1994,15(3):373-378.
[129]KOWSKA A S.Interaction of drugs with bovine and human serum albumin[J].J.Mol.Struct.,2002,614:227-232.
[130]ROSS D P,SUBRAMANIAN S.Thermodynamics of protein association reactions:forces contributing to stability[J].Biochem,1981,20:3096-3102.
[131]KLOTZ I M,URQUHART J M.The binding of organic ions by proteins.Effect of temperature [J].J.Am.Chem.Soc.,1949,71:847-851.
[132]TANFORD C.Physical Chemistry of Macromolecules[M].John Wiley and Sons.New York,1971,526
[133]TANFORD C.The Hydrophobic Effect:Formation of Micciies and Biological Membranes[M].New York:John Wiley and Sons,1973,12.
[134]MOHAMMED H R,TORU M,TOMOKO O,et.al.Study of interaction of carprofen and its enantiomers with human serum albumin-I mechanism of binding studied by dialysis and spectroscopic methods[J].Biochem.Pharm.,1993,46(10):1721-1731.
[135]ORWCORA J,BOCHS B.In silico prediction of drug-binding strengths to human serum albumin[J].J.Chromatogr.B:Biomed.Appl.,1996,677(1):1-28.
[136]GONZALEZ J Z,ACQUOTEH J,CAYRE I.Fluorescence quenching studies on binding fluoreno-9-spiro-oxazolidinedione to human serum albumin[J].Chem.Biol.Interactions,1992,84:221-228.
[137]LLOYD J B F.Synchronized excitation of fluorescence emission spectra[J].Nature(Phys.Sci.),1971,231:64-65.
[138]MILLER J N.Recent advances in molecular luminescence analysis[J].Proc.Anal.Div.Chem.Soc.,1979,16:203-208.
[139]BRUSTEIN E A,VEDENKINA N S,IRKOVA M N.Fluorescence and the location of tryptophan residues in protein molecules[J].Photochem.Photobiol.,1973,18:263-279.
[140]陈晓波,康栋国,李崧等.利福平与人血清白蛋白作用的荧光光谱[J].光谱学与光谱分析, 2006,26(4):674-677.
[141]卞伟,卫艳丽,王亚萍等.荧光法研究咖啡因和茶碱与牛血清白蛋白相互作用[J].光谱学与光谱分析,2006,26(3):505-508.
[142]毕淑云,丁兰,宋大干等.几种抗生素与人血清白蛋白结合反应的研究[J].化学学报,2005.63:2169-2173.
[143]BI S Y,SONG D Q,TIAN Y,et al.Molecular spectroscopic study on the interaction of tetracyclines with serum albumins[J].Spectrochim.Acta,Part A,2005,61:629-636.
[144]毕淑云,宋大干,魏士刚等.用荧光猝灭法研究人血清白蛋白与吲哚美辛的结合反应[J].吉林大学学报(理学版),2006,44:493-496.
[145]QI Z D,ZHANG Y,LIAO F L,et al.Probing the binding of morin to human serum albumin by optical spectroscopy[J].J.Pharm.Biomed.Anal.,2008,46:699-706.
[146]QI Z D,ZHOU B,XIAO Q,et al.Interaction of rofecoxib with human serum albumin:Determination of binding constants and the binding site by spectroscopic methods[J].J.Photochem.Photobiol.A,2008,193:81-88.
[147]SULKOWSKA A,MACIAZEK-JURCZYK M,BOJKO B,et al.Competitive binding of phenylbutazone and colchicine to serum albumin in multidrug therapy:A spectroscopic study[J].J.Mol.Struct.,2008,881:97-106.
[148]王家恒,胡忠阳,张江义等.诺氟沙星-Tb~(3+)-牛血清白蛋白相互作用的荧光光谱研究[J].分析试验室,2008,27:81-83.
[149]杨曼曼,席小莉,杨频.用荧光猝灭和荧光加强两种理论研究喹诺酮类新药与白蛋白的作用[J].高等学校化学学报,2006,27(4):687-691.
[150]张国文,阙青民,潘军辉.山姜素与脱氧核糖核酸的相互识别研究[J].光谱学与光谱分析,2008,28(8):1910-1913.
[151]BI S Y,QIAO C Y,SONG D Q,et al.Study of interactions of flavonoids with DNA using acridine orange as a fluorescence probe[J].Sens.Actuators B,2006,119:199-208.
[152]费丹,王兴明,黎泓波等.亚甲基蓝与鲱鱼精DNA相互作用的光谱法研究[J].化学学报,2008,66:443-448.
[153]徐伟民,王志颖,干宁.氢氯噻嗪与DNA相互作用的光谱研究[J].光谱实验室,2006,23:509-512.
[1]QI Z D,ZHOU B,XIAO Q,et al.Interaction of rofecoxib with human serum albumin: Determination of binding constants and the binding site by spectroscopic methods[J].J.Photochem.Photobiol.,A,2008,193:81-88.
[2]VALLNER J J.Binding of drugs by albumin and plasma protein[J].J.Pharm.Sci.,1977,66:447-465.
[3]SUDLOW G,BIRKETT D J,WADE D N.The Characterization of Two Specific Drug Binding Sites on Human Serum Albumin[J].Mol.Pharmacol.,1975,11:824-832.
[4]徐岩,沈含熙,黄汉国.利用标记药物布洛芬及保泰松研究萘啶酸与血清白蛋白的结合作用[J].高等学校化学学报,1996,12(7):1855-1858.
[5]QI Z D,ZHANG Y,LIAO F L,et al.Probing the binding of morin to human serum albumin by optical spectroscopy[J].J.Pharm.Biomed.Anal.,2008,46:699-706.
[6]LERMAN L S.Structural considerations in the interaction of DNA and acridines[J].J.Mol.Biol.,1961,3:18-30.
[7]STEINER R T,WEINRYB L.Excited States of Protein and Nucleic Acid[M].Plenum Press,New York,1971,40.
[8]CHEN G Z.Fluorescence Analytical Method[M].2nd Ed.,Science Press,Beijing,1990:122.
[9]DEWEY T G.Biophysical and Biochemical Aspects of Fluorescence Spectroscopy[M].Plenum,New York,1991:1-41.
[10]LAKOWICA J R,WEBER G.Quenching of fluorescence by oxygen.Probe for structural fluctuations in macromolecules[J].Biochem.,1973,12:4161-4170.
[11]WARE W R.Oxygenquenchixg of fluorescence in solution:ax experimental study of the diffusion process[J].J.Phys.Chem.,1962,66:455-458.
[12]SILVA D,CORTEZ C M,LOURO S R W.Chlorpromazine interactions to sera albumins:A study by the quenching of fluorescence[J].Spectrochim.Acta Part A,2004,60:1215-1223.
[13]FENG X Z,LIN Z,YANG L J,et al.Investigation of the Interaction between Acridine Orange and Bovine Serum Albumin[J].Talanta,1998,47:1223-1229.
[14]LAKOS Z,SZARKA A,KOSZORBS L,et al.Quenching-resolved emission anisotropy:a steady state fluorescence method to study protein dynamics[J].J.Photochem.Photobiol.B,1995,27:55-60.
[15]YI P G,YU Q S,SHANG Z C,et al.The reaction mechanism between ofloxacin and bovine serum albumin[J].Acta.Pharm.Sinica.,2000,35:774-777.
[16]BI S Y,QIAO C Y,SONG D Q,et al.Study of interactions of flavonoids with DNA using acridine orange as a fluorescence probe[J].Sensors and Actuators B,2006,119:199-208.
[17]EPPS D E,RAUB T J,KEZDY F J.A general,wide-rage spectrofluorometric method for measuring the site-specific affinities of drugs toward human serum albumin[J].Anal.Biochem.,1995,227(2):342-350.
[18]HAQ I.Thermodynamics of drug-DNA interactions[J].Arch.Biochem.Biophys.,2002,403:1-15.
[19]WYMAN T B,NICOL F,ZELPHATI O,et al.Design,synthesis,and characterization of a cationic peptide that binds to nucleic acids and permeabilizes bilayers[J].Biochem.,1997,36(10):3008-3017.
[20]IZUMRUDOV V A,ZHIRYAKOVA M V,KUDAIBERGENOV S E.Ethidium Bromide as a Promising Probe for Studying DNA Interaction with Cationic Amphiphiles and Stability of the Resulting Compleces[J].Biopolymers,2000,52,94-108.
[21]LLERES D,CLAMME J P,DAUTY E,et al.Investigation of the stability of dimeric cationic surfactant/DNA complexes and their interaction with model membrane systems[J].Langmuir,2002,18(26):10340-10347.
[22]IZUMRUDOV V A,ZHIRYKOVA M V,GOULKO A A.Ethidium bromide as a promising probe for studying DNA interaction with cationic amphiphiles and stability of the resulting complexes [J].Langmuir,2002,18(26):10348-10356.
[23]REICHMANN M E,RICE S A,THOMAS C A,et al.A further examination of the molecular weight and size of deoxypentose nucleic acid[J].J.Am.Chem.Soc.,1954,76:3047-3053.
[24]Marmur J.A procedure for the isolation of deoxyribonucleic acid from microorganism[J].J.Mol.Biol.1961,3:208-218.
[25]MAHLER H R,KLINE B,MEHROTRA B D.Some observations on the hypochromism of DNA [J].J.Mol.Biol.,1964,9:801-811.
[26]LONG E C,BARTON J K.Spectroscopic and electrochemical studies of the interaction between fuchsin basic DNA[J].Account Chem.Res.,1990,23(9):271-273.
[27]UDENFRIEND S,ZALTZMAN P.Fluorescence characteristics of purines,pyrimidines,and their derivatives:measurement of guanine in nucleic acid hydrolyzates[J].Anal.Biochem.,1962,3:49-59.
[28]靳兰,杨频,李青山.荧光法研究手性金属配合物与DNA的作用机理[J].高等学校化学学报,1996,17(9):1345-1348.
[29] ROSENTHAL H E. A graphic method for the determination and presentation of binding parameters in a complex system [J]. Anal. Biochem., 1967, 20 (3): 525-532.
[30] MESPLET N, MORIN P, RIBET J P. Spectrofluorimetric study of eflucimibe-y-cyclodextrin inclusion complex [J]. Eur. J. Pharm. Biopharm., 2005, 59: 523-526.
[1]贾秀荣,周济桂,廉乐贤等.橙皮甙与橙皮甙铜配合物生物活性的比较研究[J].广东微量元素科学,1996,3(2):53-56.
[2]KUNTIC V,BLAGOJEVI S,MALESEV D,et al.Spectrophotometrie investigation of the Cu (Ⅱ)-hesperidin complex[J].Pharmazie,1999,54(7):548.
[3]MALESEV D,RADOVIE Z,KUNTIC V,et al.Speetrophotometrie determination of hesperidin by Al(Ⅲ)-hesperidin complex in water-methanol solution[J].Anal.Lett.,1997,30(5):917-926.
[4]张甘良,汪钊,鄢洪德.生物类黄酮化合物的结构与生物活性的关系[J].生物学杂志,2005,2(1):4-7.
[5]谢鹏,张敏红.黄酮类化合物抑菌作用的研究进展[J].中国动物保健,2004,12:35-37.
[6]VISWANATHAN P,SRIRAM V,YOGEESWARAN G.Sensitive spectrophotometric assay for 3-hydroxy-substituted flavonoids,based on their binding with molybdenum,antimony,or bismuth[J].J.Agric.Food Chem.,2000,48:2802-2806.
[7]HYNES M J,MAIRTIN T,COINCEANAINN O.The kinetics and mechanisms of reactions of iron (Ⅲ) with caffeic acid,chlorogenic acid,sinapic acid,ferulic acid and naringin[J].J.Inorg.Biochem.,2004,98:1457-1464.
[8]EL-NYAAN U,HERLINGER E,JAMESON R G,et al.Anaerobic oxidation of dopamine by iron (Ⅲ)[J].J.Chem.Soc.Dalton Trans.,1997,2813-2818.
[9]EL-NYAAN U,JAMESON R F,LINERT W,et al.A kinetic study of the reaction between noradrenatine and iron(Ⅲ):an example of parallel inner-and outer-sphere electron transfer[J].J.Chem.Soc.Dalton Trans.,1998,1315-1319.
[10]LERMAN L S.Structural considerations in the interaction of DNA and acridines[J].J.Mol.Biol.,1961,3:18-30.
[11]STEINER R T,WE1NRYB L.Excited States of Protein and Nucleic Acid[M].Plenum Press,New York,1971,40.
[12]LAKOWICZ J R.Principles of Fluorescence Spectroscopy[M].Second ed.New York:Plenum Press,1999,237-259.
[13]LAKOWICA J R,WEBER G.Quenching of fluorescence by oxygen.Probe for structural fluctuations in macromolecules[J].Biochem.,1973,12:4161-4170.
[14]WARE W R.Oxygen quenching of fluorescence in solution:an experimental study of the diffusion process[J].J.Phys.Chem.,1962,66:455-458.
[15]LAKOWICZ J R.Principles of Fluorescence Spectroscopy[M].Plenum Press,New York,1983,257.
[16]SILVA D,CORTEZ C M,LOURO S R W.Chlorpromazine interactions to sera albumins a study by the quenching of fluorescence[J].Spectrochim.Acta Part A,2004,60:1215-1223.
[17]BI S Y,SONG D Q,TIAN Y,et al.Molecular spectroscopic study on the interaction of tetracyclines with serum albumins[J].Spectrochim.Acta Part A,2005,61:629-635.
[18]RABINDRANATH B,BIJAYA K S,KALYAN S G,et aI.Studies on the interaction of isoxazolcurcumin with calf thymus DNA[J].Int.J.Biol.Macromol.,2008,42:14-21.
[19]ROSS D P,SUBRAMANIAN S.Thermodynamic of protein association reactions:forces contributing to stability[J].Biochem.,1981,20:3096-3102.
[20]HAQ I.Thermodynamics of drug-DNA interactions[J].Arch.Biochem.Biophys.,2002,403:1-15.
[21]杨曼曼,杨频,席小莉.荧光染料探针与蛋白质结合的研究[J].科学通报,1997,42(12):1276-1279.
[22]郭尧君.荧光实验技术及其在分子生物学中的应用[M].北京:科学出版社,1983.
[23]CLEGG R M.Fluorescence resonance energy transfer[J].Curr.Opin.Biotechnol.,1995,6:103-110.
[24]DOS REMEDIOS C G,MOENS P D.Fluorescence resonance energy transfer spectroscopy is a reliable "ruler" for measuring structural changes in proteins.Dispelling the problem of the unknown orientation factor[J].J.Struct.Biol.,1995,115:175-185.
[25]SKLAR L A,HUDSON B S,SIMONI R D.Conjugated polyene fatty acids as fluorescent probes:binding bovine serum albumin[J].Biochem.,1977,16:5100-5108.
[26]LAKOWlCZ J R,WEBER G.Principles of Fluorescence Spectroscopy[M].Plenum Press,New York,1983,305-337.
[27]EPPS D E,RAUB T J,CAIOLFA V,et al.Determination of the affinity of drugs toward serum albumin by measurement of the quenching of the intrinsic tryptophan fluorescence of the protein[J].J.Pharm.Pharmacol.,1999,51:41-48.
[28]CYRIL L,EARL J K,SPERRY W M.Biochemists Handbook[M].London:E & FN Epon Led.Press,1961,83
[1]唐文涛.辨证治疗病毒性心肌炎85例[J].实用中医药杂志,1998,14(10):8-9.
[2]江苏新医学院.中药大辞典(上)[M].上海:上海人民出版社,1977.
[3]李正民.牛蒡子引起过敏反应报告[J].实用中医内科杂志,1994,8:37.
[4]赵华,王劲,杨松松.药用植物牛蒡化学成分和药理活性研究述要[J].辽宁中医学院学报,2005,7(2):3-4.
[5]郑国灿.牛蒡子苷对胰腺癌细胞抑制作用及其作用机理的实验研究[J].时珍国医国药,2008,10:2384-2386.
[6]HUANGD M,GUH J H,CHUEH S C,et al.Modulation of anti-adhesion molecule MUC-1 is associated with arctiin-induced growth inhibition in PC-3 cells[J].Prostate,2004,59(3):260-267.
[7]陈铁宏,黄迪.牛蒡子对Epstein-Barr病毒抗表达的抑制作用[J].中华实验和临床病毒学杂志,1994,8(4):323-326.
[8]杨子峰,刘妮,黄碧松等.牛蒡子甙元体内抗甲1型流感病毒作用的研究[J].中药材,2005,28(11):1012-1014.
[9]LIU S,Li Y,LI S,et al.An experimental research into the anti-aging effects of Radix Arctii Lappae [J].J.Tradit.Chin.Med.,2005,25(4):296-299.
[10]KARDOSOVA A,MACHOVA E.Antioxidant activity of medicinal plant polysaccharides[J].Fitoterapia,2006,77(5):367-373.
[11]CHO J Y,KIM A R,YOO E S,et al.Immunomodulatory effect of arctigenin oxide production and lymphocyte proliferation[J].J.Pharm.Pharmacol.,1995,51(11):1267-1273.
[12]CHO M K,JANG Y P,KIM Y C,et al.Arctigenin,a phenylpropanoid dibenzylbutyrolactone lignan,inhibits MAP kinases and AP-1 activation via potent MKK inhibition:the role in TNF-a inhibition[J].Int Immunopharmacol,2004,4(10-11):1419-1429.
[13]王淑静,魏永巨.牛蒡子中牛蒡苷含量的荧光法测定[A].国家自然科学基金委员会化学科学部.第二届全国生命分析化学学术报告与研讨会论文集[C].北京:,2008:19
[14]李慧义,罗淑荣.牛蒡子中木脂素的薄层扫描测定[J].色谱,1995,13(4):277-278.
[15]中国药典一部[M],北京:人民卫生出版社,2005:48.
[16]杨铭.以DNA为靶的小分子药物研究中的分子识别[J].北京医科大学学报,1998,30(2):97-100.
[17]宋凤瑞,闫存玉,刘宁等.核酸靶分子与某些中药成分的非共价作用研究[J].分析化学,2005.33:1743-1746.
[18]MARMUR J.A predure for the isolation of deoxyribonucleic acid from microorganism[J].J.Mol.Biol.,1961,3:208-218.
[19]MAHLER H R,KLINE B,MEHROTRA B D.Some observations on the hpochromism of DNA [J].J.Mol.Biol.,1964,9:801-811.
[20]PYLE A M,REHMANN J P,MESHOYRER R,et al.Mixed-ligand complexes of ruthenium(Ⅱ):factors governing binding to DNA[J].J.Am.Chem.Soc.,1989,111:3051-3058.
[21]LERMAN L S.Structural considerations in the interaction of DNA and acridines[J].J.Mol.Biol.,1961,3:18-30.
[22]STEINER R T,WEINRYB L.Excited States of Protein and Nucleic Acid[M].Plenum Press, New York,1971,40.
[23]LAKOWICZ J R.Principles of Fluorescence Spectroscopy[M].Second ed.New York:Plenum Press,1999,237-259.
[24]LAKOWlCA J R,WEBER G.Quenching of fluorescence by oxygen.Probe for structural fluctuations in macromolecules[J].Biochem.,1973,12:4161-4170.
[25]WARE W R.Oxygenquenchixg of fluorescence in solution:an experimental study of thediffusion process[J].J.Phys.Chem.,1962,66(3):455-458.
[26]ROSS P D,SUBRAMANIAN S.Thermodynamics of protein association reactions:forces contributing to stability[J].Biochem.,1981,20:3096-3102.
[27]JIANG C Q,HE J X,WANG J S.Studies on the interaction mode between polymyxin B sulfate and DNA with EB as a fluorescence probe[J].Spectroscopy and Spectral Analysis,2002,22(1):103-106.
[28]Fuller W,Warning M J.Ber.A molecular model for the interaction of ethidium bromide with deoxyribonucleic acid[J].Bet.Bunsen-Ges.Phys.Chem.1964,68,805-808.
[29]LEPECQ J B,PAOLETLI C.A new fluorometric method for RNA and DNA determination.Anal.Biochem.,1966,17:100-107.
[30]REINHARDT C G,KRUGH T R.A comparative study of ethidium bro-mide complexes with dinucleotides and DNA:direct evidence for intercalation and nucleic acid sequence preferences[J].Biochem.,1978,17(23):4845-4853.
[31]KUMAR C V,TURNER R S.Groove binding ofa styrylcyanine dye to the DNA double helix:the salt effect[J].J.Photochem.Photobio.A:Chem.,1993,74:231-238.
[32]RAIAMANICKAM V,MOOKANDI K,VENKATESAN S,et al.Interaction of DNA with [Cr(Schii base)(H_2O)_2]ClO_4[J].Biochim.Biophys.Acta.,2000,1475:157-162.
[33]李来生,黄志兵,王宇晓等.荧光光谱法研究对-二甲氨甲基-杯[8]芳烃与DNA相互作用[J].光谱学与光谱分析,2005,25(7):1088-1092.
[34]PASTERNACK R F,BRIGANDI R A,ABRAMS M J.Interactions of porphyrins and metalloporphyrins with single-stranded poly(dA)[J].Inorg.Chem.,1990,29:4483-4486.
[35]梅文杰,刘杰,李安兴等.钌卟啉[Ru(bpy)_2(MPyTPP)Cl]~+与DNA作用的研究[J].高等学校化学学报,2002,23(11):2049-2051.
[36]PRATVIEL G,BERNADOU J,MEUNIER B.Carbon-hydrogen bonds of DNA sugar units as targets for chemical nucleases and drugs [J]. Angew. Chem. Int. Ed. Engl., 1995, 34: 746-769.
[37] KUMAR C V, TURNER R S, ASUNCION E H. Groove binding of a styrylcyanine dye to the DNA double helix: The salt effect [J]. J. Photochem. Photobiol. A: Chem., 1993, 74: 231-238.
[1]马绍宾,胡志浩.小檗科鬼臼亚科的地理分布与系统发育[J].云南植物研究,1997,19(1):48-56.
[2]KING L S,SULLIVAN M.The similarity of the effect of podophyllin and colchicine and their use in the treatment of condylomata acuminata[J].Science,1946,104:244-245.
[2]杨显志,邵华,张玲琪等.鬼臼毒素资源研究现状[J].中草药,2001,32(11):1042-1044.
[3]潘勤.鬼臼毒素类环木脂素的甲醚衍生物[J].国外医药-植物药分册,1996,11(2):73-74.
[4]LEE K H,SCOTT A B,MASAMI M,et al.Antitumor agents.111 New 4-hydroxylated and 4-halogenated anilino derivatives of 4'-demet hylepipodophyllotoxin as potent inhibitors of human DNA topoisomerase Ⅱ[J].J.Med.Chem.,1990,33(5):1364-1368.
[5]朱晓亮,曾抗,陈飞龙.紫外分光光度法测定鬼臼毒素壳聚糖膜剂中鬼臼毒素的含量[J].中国现代医学杂志,2005,15(5):769-771.
[6]陈燕,德吉,刘倩伶等.桃儿七果实中鬼臼毒素的鉴别和含量测定[J].华西药学杂志,2009,24(1):93-94.
[7]张丽艳,万明香,何顺志等.HPLC法测定贵州产八角莲属药材中鬼臼毒素的含量[J].药物分析杂志,2009.29:202-204.
[8]张杰,周春山,向大雄等.高效液相色谱法测定小八角莲中鬼臼毒素含量[J].湖南中医杂志,2007,23(4):200-201.
[9]马守栋,孟庆礼.荧光分光光度法测定鬼臼毒素的含量[J].中国医院药学杂志,1998,18,(4):162-163.
[10]GLINSKI M B,FREED J C,DURST T J.Novel stereoselective synthesis of(-)-enterolactone employing chiral unsaturated lactam[J].Org.Chem.,1987,52:2749-2753.
[11]王庆文,杨玉桓,高鸿宾.有机化学中的氢键问题[M].天津:天津大学出版社,1993:10-12.
[12]徐国钧.中国药材学[M].北京:中国医药科技出版社,1993:24.
[13]BAI H,LI W,KOIKE B K,et al.A Novel Biflavonoid from Roots of Glycyrrhiza uralensis Cultivated in China[J].Chemical and Pharmaceutical Bulletin,2003,51(9):1095-1097.
[14]张雪辉,赵元芬,陈建民.甘草中总黄酮的含量测定[J].中国中药杂志,2001,26(11):746-749.
[15]何三民,石森林.HPLC法测定甘草中甘草素、异甘草素、甘草苷的含量[J].中草药,2003,34(7):618-619.
[16]丁选胜,戴德哉.甘草苷对四氯化碳肝脏毒性的保护作用[J].中草药,2003,34(12):1122-1123.
[17]赵志宇,王卫星,郭洪祝等.甘草苷对慢性应激抑郁模型大鼠的抗抑郁作用.中国临床康复,2006,10(27):69-72.
[18]王小强.薄层光密度法测定甘草中甘草甙的含量[J].药物分析杂志,1990,10(6):351-352.
[19]段天璇,于密密,刘春生等.HPLC法同时测定甘草指纹图谱暨甘草苷、甘草酸含量[J].中成药,2006,28(2):161-165.
[20]靳凤云,贺祝英,赵杨等.HPLC测定华盖散传统汤剂与颗粒汤剂中盐酸麻黄碱、苦杏仁苷、甘草酸、甘草苷的含量[J].中成药,2008,30(1):80-87.
[21]犹卫,郑志祥,高作宁.甘草苷在悬汞电极上的电化学行为及方法研究[J].分析试验室.2008,27(7):5-8.
[22]陈亚东,曹秀兰.高山红景天对小鼠耐缺氧、抗疲劳及耐低温作用的影响[J].中国中医药科技,2002,9(3):157-158.
[23]刘志伟,吴谋成,陈平.菱叶红景天对小鼠学习记忆影响及耐缺氧实验[J].营养学报,2003,25(1):101-104.
[24]骆传环,高月,王作华等.红景天多糖的抗放实验的研究[J].中华放射医学与防护杂志,1999,14(5):340-341.
[25]中国医学科学院.中国协和医科大学医学信息研究所.医学主题词表[M].北京:中国计量出版社,2002.
[26]赵文,蒋东升,边庆荣等.蔷薇红景天提取液对小鼠免疫功能的影响[J].卫生毒理学杂志,2000,14(3):159.
[27]余国祥,蔡鹰.红景天生脉口服液抗实验性心律失常的作用[J].云南中医中药杂志,2003,24(4):37-38.
[28]毛静远,王化良,赵如菊等.红景天胶囊治疗冠心病心绞痛临床研究[J].中国医药学报,2002,17(8):480-483.
[29]李君,陈志,李建民等.红景天的研究进展[J].云南农业大学学报,2007,22:61-64.
[30]张早华,储戟农,林文翰等.高效液相色谱法测定红景天注射液中红景天苷的含量[J].中国中医药信息杂志,2005,12(11):51-52.
[31]杨晓艳,芦启琴,张晓峰.HPLC法测定红景天根皮中红景天苷的含量.分析试验室,2008,27:305-306.
[32]安伟建,陈冠,王文彤等.HPLC法测定不同产地红景天属植物中红景天苷[J].中草药,2007,38(7):1096-1097.
[33]孟健,王淑芬,韩飞等.SPE-HPLC测定注射用红景天(冻干)中红景天苷和酪醇的含量[J].中国中药杂志,2006,31(2):119-121.
[34]陈志英,陈玉婷,王丹红.HPLC测定不同品种红景天中红景天苷的含量[J].2006,31(11):939-941.
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