高住低训对红细胞CD_（35）的影响及服用灵芝提取物的作用
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摘要
红细胞具有天然免疫功能,是机体重要的免疫细胞。高住低训红细胞免疫研究才起步,关于高住低训中红细胞CD35数量的变化以及灵芝对红细胞CD35数量和活性变化影响的研究还未见报道。
目的:
一、本实验通过观察4周2500米高住低训中人体红细胞CD35的数量和活性的变化,探讨高住低训对人体红细胞CD35的影响;二、通过观察4周2500米高住低训过程中,人体红细胞CD35数量和活性变化,探讨不同剂量灵芝提取物对高住低训中人体红细胞CD35的影响。
研究方法:
第一部分、以16名北京体育大学体育系足球专项男生为研究对象,随机分为两组,高住低训组和低住低训组。高住低训组每晚入住低氧房(15.4%氧浓度相当于海拔2500米,常压)10小时,每周2次低氧房72%最大摄氧量强度的功率自行车训练30分钟,其余活动均在常氧下进行,连续4周;低住低训组在常氧(20.9%,常压)居住,每周2次常氧环境下80%最大摄氧量强度的功率自行车训练30分钟,连续4周。高住低训组和低住低训组平时的专项训练、学习、饮食、作息等活动基本一致。检测入住低氧房前、入住10小时、2周末、3周末、4周末红细胞CD35数量和活性;第二部分、以24名足球专项男生为研究对象,随机分为三组。分别为低剂量组(每日服药2.5g)、高剂量组(每日服药5g)和对照组,每组各8人,均为高住低训。每晚入住低氧房(15.4%氧浓度相当于海拔2500米,常压)10小时,每周2次低氧房72%最大摄氧量强度的功率自行车训练30分钟,其余活动均在常氧下进行,连续4周。检测吃药2周前,入住低氧房前、入住10小时、2周末、3周末、4周末红细胞CD35数量和活性。
研究结果表明:
1.高住低训和低住低训对人体红细胞CD35数量表达的影响不如对红细胞CD35活性的影响明显。
2.高住低训比低住低训对红细胞免疫活性的影响更显著,高住低训暴露3周末运动员机体表现出临床上的继发性红细胞免疫功能低下,暴露4周末有所好转。
3.高剂量灵芝胶囊可以明显影响红细胞CD35数量的表达,并且高剂量灵芝胶囊比低剂量灵芝胶囊对红细胞CD35的活性的影响更明显,可以调节高住低训实验中出现的运动员红细胞继发性免疫低下的现象。
Objective: Erythrocyte has been approved that it has immune function. In order to observe the changes of the number and activity on erythrocyte complement receptor 1 in athletes during living high-training low (LHTL) at 15.4% O2 concentration,and the effects of ganoderma lucidum on the changes of the number and activity on erythrocyte complement receptor 1(CR1,CD35) in Soccer Players during living high-training low, we arranged two experiments,each lasted four weeks.
Methods: Section I: sixteen male college soccer players were divided into two groups randomly: LHTL group (n=8) trained at sea level and lived for 4 weeks (10h /d, 7d /wk) in norm baric hypoxic rooms with O2 15.4% (simulating at altitude of 2500m), while control (Con) group (n=8) trained and lived at sea level. Additionally LHTL group exercised on the cycle ergometer at 72% O2max intensity respectively in hypoxic rooms (30 min/time, 2 times/week), but control group exercised on the cycle ergometer at 80% O2max intensity respectively at sea level.Section II: twenty-four male college soccer players were divided into three groups randomly:low dose(LD) group(n=8),high dose(HD)group(n=8) and control(Con)group, trained at sea level and lived for 4 weeks (10h /d , 7d /wk ) in norm baric hypoxic rooms with O2 15.4% ( simulating at altitude of 2500m ).At the same time,LD group took ten ganoderma lucidum capsules a day and HD group took twenty ganoderma lucidum capsules a day,while Con group just took non-capsules everyday. We measured the soccer players’s red blood cell immune function by Feng Guo’s method and by flow cytometry.
Conclusion: 1. Although there was not a significant influence on the number of erythrocyte complement receptor type 1 in LHTL group and Con group, the changes of red blood cell nature immune activity were evidently different between the two groups. During 3 weeks LHTL, the red blood cell immune activity of LHTL group showed a clinical secondary decline compare to before hypoxic exposure.2.High dose of ganoderma lucidum capsules could significantly increase the number of CR1 more than low dose and Con group. Compared with low dose and Con group, high dose of ganoderma lucidum capsules had better effects on activity of erythrocyte complement receptor 1.
目的:
一、本实验通过观察4周2500米高住低训中人体红细胞CD35的数量和活性的变化,探讨高住低训对人体红细胞CD35的影响;二、通过观察4周2500米高住低训过程中,人体红细胞CD35数量和活性变化,探讨不同剂量灵芝提取物对高住低训中人体红细胞CD35的影响。
研究方法:
第一部分、以16名北京体育大学体育系足球专项男生为研究对象,随机分为两组,高住低训组和低住低训组。高住低训组每晚入住低氧房(15.4%氧浓度相当于海拔2500米,常压)10小时,每周2次低氧房72%最大摄氧量强度的功率自行车训练30分钟,其余活动均在常氧下进行,连续4周;低住低训组在常氧(20.9%,常压)居住,每周2次常氧环境下80%最大摄氧量强度的功率自行车训练30分钟,连续4周。高住低训组和低住低训组平时的专项训练、学习、饮食、作息等活动基本一致。检测入住低氧房前、入住10小时、2周末、3周末、4周末红细胞CD35数量和活性;第二部分、以24名足球专项男生为研究对象,随机分为三组。分别为低剂量组(每日服药2.5g)、高剂量组(每日服药5g)和对照组,每组各8人,均为高住低训。每晚入住低氧房(15.4%氧浓度相当于海拔2500米,常压)10小时,每周2次低氧房72%最大摄氧量强度的功率自行车训练30分钟,其余活动均在常氧下进行,连续4周。检测吃药2周前,入住低氧房前、入住10小时、2周末、3周末、4周末红细胞CD35数量和活性。
研究结果表明:
1.高住低训和低住低训对人体红细胞CD35数量表达的影响不如对红细胞CD35活性的影响明显。
2.高住低训比低住低训对红细胞免疫活性的影响更显著,高住低训暴露3周末运动员机体表现出临床上的继发性红细胞免疫功能低下,暴露4周末有所好转。
3.高剂量灵芝胶囊可以明显影响红细胞CD35数量的表达,并且高剂量灵芝胶囊比低剂量灵芝胶囊对红细胞CD35的活性的影响更明显,可以调节高住低训实验中出现的运动员红细胞继发性免疫低下的现象。
Objective: Erythrocyte has been approved that it has immune function. In order to observe the changes of the number and activity on erythrocyte complement receptor 1 in athletes during living high-training low (LHTL) at 15.4% O2 concentration,and the effects of ganoderma lucidum on the changes of the number and activity on erythrocyte complement receptor 1(CR1,CD35) in Soccer Players during living high-training low, we arranged two experiments,each lasted four weeks.
Methods: Section I: sixteen male college soccer players were divided into two groups randomly: LHTL group (n=8) trained at sea level and lived for 4 weeks (10h /d, 7d /wk) in norm baric hypoxic rooms with O2 15.4% (simulating at altitude of 2500m), while control (Con) group (n=8) trained and lived at sea level. Additionally LHTL group exercised on the cycle ergometer at 72% O2max intensity respectively in hypoxic rooms (30 min/time, 2 times/week), but control group exercised on the cycle ergometer at 80% O2max intensity respectively at sea level.Section II: twenty-four male college soccer players were divided into three groups randomly:low dose(LD) group(n=8),high dose(HD)group(n=8) and control(Con)group, trained at sea level and lived for 4 weeks (10h /d , 7d /wk ) in norm baric hypoxic rooms with O2 15.4% ( simulating at altitude of 2500m ).At the same time,LD group took ten ganoderma lucidum capsules a day and HD group took twenty ganoderma lucidum capsules a day,while Con group just took non-capsules everyday. We measured the soccer players’s red blood cell immune function by Feng Guo’s method and by flow cytometry.
Conclusion: 1. Although there was not a significant influence on the number of erythrocyte complement receptor type 1 in LHTL group and Con group, the changes of red blood cell nature immune activity were evidently different between the two groups. During 3 weeks LHTL, the red blood cell immune activity of LHTL group showed a clinical secondary decline compare to before hypoxic exposure.2.High dose of ganoderma lucidum capsules could significantly increase the number of CR1 more than low dose and Con group. Compared with low dose and Con group, high dose of ganoderma lucidum capsules had better effects on activity of erythrocyte complement receptor 1.
引文
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