昆明杯冠藤和Pfaffia glomerate的化学成分研究
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摘要
昆明杯冠藤(Cynanchum wallichii Wight.)又名断节参,是萝藦科鹅绒藤属植物。产于我国四川、贵州、云南以及广西等地,并分布于印度。民间用其治疗风湿关节炎、及跌打损伤,云南成药“虎力散”用其为主药。其主要化学成分为C-(21)甾体类成分。从昆明杯冠藤(Cynanchum wallichi Wight.)根的95%乙醇提取物中,分离得到了21个C_(21)甾体类化合物,鉴定了其中18个C_(21)甾体类化合物。分别为:断节参苷B(1),断节参苷C(2),断节参苷D(3),断节参苷E(4),断节参苷F(5),断节参苷G(6),断节参苷H(7),断节参苷I(8),断节参苷J(9),断节参苷K(10),断节参苷L(11),断节参苷M(12),高达庭(13),加加明(14),青阳参苷A(15),青阳参苷B(16),白首乌新苷A(17),青阳参苷M(18)。其中化合物1-12为新化合物。化合物15-18为首次从该种植物中分离得到的化合物。
对其中的16个化合物进行了细胞生长抑制活性测试,化合物4、6、13、16对HL-60细胞显示出一定程度的抑制活性。化合物6和16对PC3细胞显示出一定程度的抑制活性。
苋科Amaranthaceae法菲亚属Pfaffia Pedersen植物主要药用部位是根。根据文献报道,Pfaffia glomerate具有壮阳、抗炎和降血糖等功效,是当地民间一种重要草药。从该属植物Pfaffia glomerate的干燥根的甲醇提取物中共分离了41个化合物,并鉴定了其中的37个化合物。分别为:Glomerate acid B(1),Glomerate acid C(2),Glomerate acid D(3),Glomerate acid E(4),Glomoside A-M(5-17),20-羟基蜕皮甾酮(18),蕨甾酮(19),毒甾酮(20),罗汉松甾酮(21),二羟红苋甾酮(22),齐墩果酸-3-O-β-D-葡萄糖醛酸苷(23),齐墩果酸-3-O-β-D-葡萄糖醛酸苷甲酯(24),竹节参苷甲酯(25),竹节参苷(26),落葵安迪苷A_2(27),法菲亚苷B(28),齐墩果酸(29),20(29)烯-30-去甲齐墩果酸(30),齐墩果酸-3-O-β-D-葡萄糖苷(31),29-羟基齐墩果酸(32),三对节萜酸(33),22-氧代-20-羟基蜕皮甾酮(34),11-氧代-齐墩果酸-28-O-β-D-葡萄糖苷(35),22β-羟基齐墩果酸(36),20α-羟基-29-去甲齐墩果酸(37)。其中化合物1-10为新化合物,化合物11-17为新酯类化合物。
Cynanchum wallichii Weight,Duanjieshen,is a traditional Chinese medicine distributed extensively over southwest China.It is used as the primary drug in the famous Chinese prescription "hulisan",which is used to treat arthrophlogosis and injury from fall or fracture.
In our investigation,21 compounds were isolated from the 95%ethanol extract of C. wallichii Weight,18 of which were identified as wallicoside B(1),wallicoside C(2), wallicoside D(3),wallicoside E(4),wallicoside F(5),wallicoside G(6),wallicoside H(7), wallicoside I(8),wallicoside J(9),wallicoside K(10),wallicoside L(11),wallicoside M(12), caudatin(13),gagamine(14),otophylloside A(15),otophylloside B(16),cynanauriculoside A(17),otophylloside M(18)。Among them,compounds 1-12 were new compounds, compounds 15-18 were isolated from Cynanchum wallichii Weight for the first time.
The growth inhibitory activities of some C_21 steroidal glycosides in vitro against cancer cell HL-60 and PC3 were measured.Compounds 4,6,13 and 16 showed certain activities against the cancer cell HL-60,and compounds 6 and 16 showed certain activities against the cancer cell PC3.
Pfaffia glomerate belongs to the family of Amaranthceace,genus of Pfaffia Pedersen. Pharmacological experiments show that the extracts from Pfaffia glomerate have the effects of enhancing male sexual capacity,anti-inflammatory,analgesic,sedative and anti-diabetes. 41 compounds were isolated from the 95%ethanol extract of P.glomerate,37 of which were identified as:glomerate acid B(1),glomerate acid C(2),glomerate acid D(3),glomerate acid E(4),glomoside A-M(5-17),20-hydroxyecodysone(18),pterosterone(19),taxisterone (20),podecdysone C(21),dihydrorubrosterone(22),calenduloside E(23),6'-ο-methyl calenduloside E(24),chikusetsusaponinⅣa methyl ester(25),chikusetsusaponinⅣa(26), boussingoside A_2(27),pfaffoside B(28),oleanolic acid(29),20(29)en-30-noroleanolic acid (30),β-glucopyranosyl oleanolate(31),mesenbryanthemoidgenic acid(32),serratagenic acid (33),22-Oxo-20-hydroxyecdysone(34),11-oxo-β-glucopyranosyl oleanolate(35),22βhydroxyl-oleanolic acid(36),20α-hydroxyl-29-noroleanolic acid(37).Among them, compounds 1-10 were new compounds,compounds 11-17 were new ester compounds.
对其中的16个化合物进行了细胞生长抑制活性测试,化合物4、6、13、16对HL-60细胞显示出一定程度的抑制活性。化合物6和16对PC3细胞显示出一定程度的抑制活性。
苋科Amaranthaceae法菲亚属Pfaffia Pedersen植物主要药用部位是根。根据文献报道,Pfaffia glomerate具有壮阳、抗炎和降血糖等功效,是当地民间一种重要草药。从该属植物Pfaffia glomerate的干燥根的甲醇提取物中共分离了41个化合物,并鉴定了其中的37个化合物。分别为:Glomerate acid B(1),Glomerate acid C(2),Glomerate acid D(3),Glomerate acid E(4),Glomoside A-M(5-17),20-羟基蜕皮甾酮(18),蕨甾酮(19),毒甾酮(20),罗汉松甾酮(21),二羟红苋甾酮(22),齐墩果酸-3-O-β-D-葡萄糖醛酸苷(23),齐墩果酸-3-O-β-D-葡萄糖醛酸苷甲酯(24),竹节参苷甲酯(25),竹节参苷(26),落葵安迪苷A_2(27),法菲亚苷B(28),齐墩果酸(29),20(29)烯-30-去甲齐墩果酸(30),齐墩果酸-3-O-β-D-葡萄糖苷(31),29-羟基齐墩果酸(32),三对节萜酸(33),22-氧代-20-羟基蜕皮甾酮(34),11-氧代-齐墩果酸-28-O-β-D-葡萄糖苷(35),22β-羟基齐墩果酸(36),20α-羟基-29-去甲齐墩果酸(37)。其中化合物1-10为新化合物,化合物11-17为新酯类化合物。
Cynanchum wallichii Weight,Duanjieshen,is a traditional Chinese medicine distributed extensively over southwest China.It is used as the primary drug in the famous Chinese prescription "hulisan",which is used to treat arthrophlogosis and injury from fall or fracture.
In our investigation,21 compounds were isolated from the 95%ethanol extract of C. wallichii Weight,18 of which were identified as wallicoside B(1),wallicoside C(2), wallicoside D(3),wallicoside E(4),wallicoside F(5),wallicoside G(6),wallicoside H(7), wallicoside I(8),wallicoside J(9),wallicoside K(10),wallicoside L(11),wallicoside M(12), caudatin(13),gagamine(14),otophylloside A(15),otophylloside B(16),cynanauriculoside A(17),otophylloside M(18)。Among them,compounds 1-12 were new compounds, compounds 15-18 were isolated from Cynanchum wallichii Weight for the first time.
The growth inhibitory activities of some C_21 steroidal glycosides in vitro against cancer cell HL-60 and PC3 were measured.Compounds 4,6,13 and 16 showed certain activities against the cancer cell HL-60,and compounds 6 and 16 showed certain activities against the cancer cell PC3.
Pfaffia glomerate belongs to the family of Amaranthceace,genus of Pfaffia Pedersen. Pharmacological experiments show that the extracts from Pfaffia glomerate have the effects of enhancing male sexual capacity,anti-inflammatory,analgesic,sedative and anti-diabetes. 41 compounds were isolated from the 95%ethanol extract of P.glomerate,37 of which were identified as:glomerate acid B(1),glomerate acid C(2),glomerate acid D(3),glomerate acid E(4),glomoside A-M(5-17),20-hydroxyecodysone(18),pterosterone(19),taxisterone (20),podecdysone C(21),dihydrorubrosterone(22),calenduloside E(23),6'-ο-methyl calenduloside E(24),chikusetsusaponinⅣa methyl ester(25),chikusetsusaponinⅣa(26), boussingoside A_2(27),pfaffoside B(28),oleanolic acid(29),20(29)en-30-noroleanolic acid (30),β-glucopyranosyl oleanolate(31),mesenbryanthemoidgenic acid(32),serratagenic acid (33),22-Oxo-20-hydroxyecdysone(34),11-oxo-β-glucopyranosyl oleanolate(35),22βhydroxyl-oleanolic acid(36),20α-hydroxyl-29-noroleanolic acid(37).Among them, compounds 1-10 were new compounds,compounds 11-17 were new ester compounds.
引文
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