不同杂交晚稻品种生育后期根叶衰老生理对限水增N的响应
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摘要
为了探讨不同杂交稻品种后期衰老叶片及根系生理指标变化差异,选用不同杂交稻品种在抽穗后,采用常规水分管理(CK)、限水(T)和限水增N(T+N)处理,研究后期叶片和根系生理响应。为水稻抗早衰品种的选用、及其后肥水管理提供依据。不同杂交稻品种后期叶片和根系衰老生理间存在差异,结果表明：
不同杂交稻品种后期叶面积指数对T和T+N处理响应存在差异,但大都表现对T和T+N处理敏感。叶绿素含量、Rubisco活性和Pn不同品种间衰减节律有明显差异,表现在不同品种对T和T+N处理的敏感程度不同,天优998、隆平601和汕优63的Pn均对T和T+N处理敏感,而其余品种则表现不敏感；Rubisco活性除隆平601对T和T+N处理均敏感外,其余品种大都只对T+N处理敏感。
POD、CAT、SOD和ASA-POD活性以及MDA含量动态变化不同,不同品种对T和T+N处理的响应存在差异,表现在响应时间和敏感程度不同,中优838根系和叶片POD活性和MDA含量均对T处理表现敏感,而其余供试品种则有不同的敏感程度；根系与叶片不同抗氧化物保护酶活性对T和T+N处理响应的敏感程度品种间也存在差异。
不同供试品种C、N代谢和根系活力差异表现在衰减的起始时间和衰减的幅度及对T和T+N处理敏感程度不同,隆平601、汕优63和C两优396高效叶全N含量对T处理稍敏感,同时所有品种对T+N处理均敏感。除C两优396,其它品种GDH活性均对T处理敏感,且所有品种对T+N处理均敏感。中优838、天优998和C两优396根系伤流量下降幅度偏大,对T处理亦敏感。不同品种GDH活性对T和T+N处理敏感性存在差异,表现在两优6326和C两优396对T+N处理敏感,而除C两优396外均对T处理敏感。叶片Ar含量变化趋势品种间无明显差异,但大都表现对T+N处理敏感,T不敏感。多数品种叶片可溶性糖含量对T处理敏感,同时T+N处理可降低可溶性糖含量。
不同品种间激素含量无明显差异,处理间差异明显,T处理叶片ZR含量增高,同时CK与T+N处理较为接近。根系伤流液中ABA含量T处理的较高,T+N较低；T处理ZR含量较低,T+N较高。叶片与根系激素含量的相关程度品种间存在差异,表现在ABA含量除隆平601外均达到显著负相关；ZR含量汕优63达到显著负相关,其余品种负相但度均不显著。
In order to discuss the physiological indexes variation of later aging leaf and root during different hybrid rice combinations, we dispose the aging leaf and root with CK, T and T+N, and research their physiological response, as providing basis for breeding rice which is resistant to premature decay and later fertilizer and water management. The physiological aging processes of later aging leaf and root during different hybrid rice combinations are different. Results showed that:
The responses on T and T+N processing of leaf area index of different hybrid rice combinations are different, but mostly are sensitive to T and T+N. The Attenuation rhythms of Chlorophyll content, Rubisco activity and Pn between different combinations are obviously different, in the different sensitive degree on T and T+N between different hybrid rice combinations. The Pn of Tian you 998, Long pin 601 and shan you 63 are sensitive to T and T+N, but the rest are not sensitive; Excepting Long ping 601, the activity of Rubisco is sensitive to both T and T+N, the rest combinations are only sensitive to T+N.
Dynamic changes of the activity of POD, CAT, SOD and ASA-POD as well as the content of MDA are different, and the responses of different combinations on T and T+N are different too, in the responses time and sensitive degree are different. The activity of POD and the content of MDA in the leaf and root of Zhong you 838 are sensitive to T, but the rest of the selected combinations have different sensitive degree; the sensitive degree of antioxidants protective enzyme in leaf and root on T and T+N between different combinations is different too.
The differences of C, N metabolism and root vigor are performed in the starting time and the amplitude of attenuation and the sensitive degree on T and T+N. The full content of N in efficient leaves of Long ping 601, Shan you 63 and C Liang you 396 is slightly sensitive to T, and all the combinations are sensitive to T+N. Excepting C Liang you 396, the activity of GDH of the rest combinations are all sensitive to T, and all the combinations are sensitive to T+N. The decline of injury flow of Zhong you 838, Tian you 998 and C Liang you 396 in root is big, and is sensitive to T. The sensitivity of GDH of different combinations on T and T+N is different, for example, Liang you 6326 and C Liang you 396 are sensitive to T+N, but, excepting C Liang you, the others are all sensitive to T. The variation trend of Ar between different combinations is not obviously different, but mostly is sensitive to T+N, not sensitive to T. The soluble sugar content in most combination blades is sensitive to T, and T+N could decrease the soluble sugar content.
Hormone levels between different combinations are without obviously different. But they are clearly different between different disposes. The content of ZR increased when dealing with T, and it is relatively close when dealing with CK or T+N. The content of ABA in the root injury flow is higher when dealing with T than dealing with T+N; the content of ZR is opposite. The related degree of hormone levels in leaf and root between different combinations is different. It showed that the contents of ABA in the selected combinations, excepting Long ping 601, were significantly and negatively correlated. The contents of ZR in Shan you63 were significantly and negatively correlated. The rest were all negatively correlated, but not significantly.
不同杂交稻品种后期叶面积指数对T和T+N处理响应存在差异,但大都表现对T和T+N处理敏感。叶绿素含量、Rubisco活性和Pn不同品种间衰减节律有明显差异,表现在不同品种对T和T+N处理的敏感程度不同,天优998、隆平601和汕优63的Pn均对T和T+N处理敏感,而其余品种则表现不敏感；Rubisco活性除隆平601对T和T+N处理均敏感外,其余品种大都只对T+N处理敏感。
POD、CAT、SOD和ASA-POD活性以及MDA含量动态变化不同,不同品种对T和T+N处理的响应存在差异,表现在响应时间和敏感程度不同,中优838根系和叶片POD活性和MDA含量均对T处理表现敏感,而其余供试品种则有不同的敏感程度；根系与叶片不同抗氧化物保护酶活性对T和T+N处理响应的敏感程度品种间也存在差异。
不同供试品种C、N代谢和根系活力差异表现在衰减的起始时间和衰减的幅度及对T和T+N处理敏感程度不同,隆平601、汕优63和C两优396高效叶全N含量对T处理稍敏感,同时所有品种对T+N处理均敏感。除C两优396,其它品种GDH活性均对T处理敏感,且所有品种对T+N处理均敏感。中优838、天优998和C两优396根系伤流量下降幅度偏大,对T处理亦敏感。不同品种GDH活性对T和T+N处理敏感性存在差异,表现在两优6326和C两优396对T+N处理敏感,而除C两优396外均对T处理敏感。叶片Ar含量变化趋势品种间无明显差异,但大都表现对T+N处理敏感,T不敏感。多数品种叶片可溶性糖含量对T处理敏感,同时T+N处理可降低可溶性糖含量。
不同品种间激素含量无明显差异,处理间差异明显,T处理叶片ZR含量增高,同时CK与T+N处理较为接近。根系伤流液中ABA含量T处理的较高,T+N较低；T处理ZR含量较低,T+N较高。叶片与根系激素含量的相关程度品种间存在差异,表现在ABA含量除隆平601外均达到显著负相关；ZR含量汕优63达到显著负相关,其余品种负相但度均不显著。
In order to discuss the physiological indexes variation of later aging leaf and root during different hybrid rice combinations, we dispose the aging leaf and root with CK, T and T+N, and research their physiological response, as providing basis for breeding rice which is resistant to premature decay and later fertilizer and water management. The physiological aging processes of later aging leaf and root during different hybrid rice combinations are different. Results showed that:
The responses on T and T+N processing of leaf area index of different hybrid rice combinations are different, but mostly are sensitive to T and T+N. The Attenuation rhythms of Chlorophyll content, Rubisco activity and Pn between different combinations are obviously different, in the different sensitive degree on T and T+N between different hybrid rice combinations. The Pn of Tian you 998, Long pin 601 and shan you 63 are sensitive to T and T+N, but the rest are not sensitive; Excepting Long ping 601, the activity of Rubisco is sensitive to both T and T+N, the rest combinations are only sensitive to T+N.
Dynamic changes of the activity of POD, CAT, SOD and ASA-POD as well as the content of MDA are different, and the responses of different combinations on T and T+N are different too, in the responses time and sensitive degree are different. The activity of POD and the content of MDA in the leaf and root of Zhong you 838 are sensitive to T, but the rest of the selected combinations have different sensitive degree; the sensitive degree of antioxidants protective enzyme in leaf and root on T and T+N between different combinations is different too.
The differences of C, N metabolism and root vigor are performed in the starting time and the amplitude of attenuation and the sensitive degree on T and T+N. The full content of N in efficient leaves of Long ping 601, Shan you 63 and C Liang you 396 is slightly sensitive to T, and all the combinations are sensitive to T+N. Excepting C Liang you 396, the activity of GDH of the rest combinations are all sensitive to T, and all the combinations are sensitive to T+N. The decline of injury flow of Zhong you 838, Tian you 998 and C Liang you 396 in root is big, and is sensitive to T. The sensitivity of GDH of different combinations on T and T+N is different, for example, Liang you 6326 and C Liang you 396 are sensitive to T+N, but, excepting C Liang you, the others are all sensitive to T. The variation trend of Ar between different combinations is not obviously different, but mostly is sensitive to T+N, not sensitive to T. The soluble sugar content in most combination blades is sensitive to T, and T+N could decrease the soluble sugar content.
Hormone levels between different combinations are without obviously different. But they are clearly different between different disposes. The content of ZR increased when dealing with T, and it is relatively close when dealing with CK or T+N. The content of ABA in the root injury flow is higher when dealing with T than dealing with T+N; the content of ZR is opposite. The related degree of hormone levels in leaf and root between different combinations is different. It showed that the contents of ABA in the selected combinations, excepting Long ping 601, were significantly and negatively correlated. The contents of ZR in Shan you63 were significantly and negatively correlated. The rest were all negatively correlated, but not significantly.
引文
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