黄酮强吸附与分子表面印迹材料的制备及特性研究
|
摘要
黄酮类化合物(flavonoids)是一类广泛存在于植物组织中并且具有多种生物活性的天然物质。现代药理学研究结果表明，黄酮类化合物具有很高的药理活性，如清除自由基、抗氧化、抗肿瘤、抗病毒及杀菌等多种功能，在天然药物化学中占很重要的地位。槲皮素(quercetin)、芦丁(rutin)和染料木素(genistein)是三种典型的多羟基黄酮类化合物，具有很高的生物活性和药理活性，它们的提取与分离在化学与生物医学领域是备受关注的研究课题。本研究通过分子设计的构思，设计制备对黄酮类物质具有强吸附作用的聚合物材料，并采用“接枝聚合与分子印迹同步进行”的新型分子表面印迹技术，制备黄酮分子表面印迹材料，以促进天然药物化合物的提取分离研究以及它们的临床应用。显然，本课题研究在生物药学与化学科学领域具有重要的科学意义。
首先，使用偶联剂γ-氨丙基三甲氧基硅烷(AMPS)，对微米级硅胶微粒进行了化学改性，制得表面带有伯胺基的改性微粒AMPS-SiO2，使用水和二甲亚砜的混合溶剂，使改性微粒AMPS-SiO2表面的氨基与溶液中的过硫酸铵构成氧化-还原引发体系，实现了乙酸乙烯酯(VAc)在硅胶微粒表面的引发接枝聚合，制得了高接枝度的接枝微粒PVAc/SiO2。经醇解，将PVAc转变为聚乙烯醇(PVA)，从而制备了高接枝度的功能接枝微粒PVA/SiO2。采用红外光谱(FTIR)、扫描电镜(SEM)及热重分析(TGA)等方法对产物微粒进行了表征。并深入研究了主要因素对VAc表面引发接枝聚合的影响。研究结果表明，采用氨基与过硫酸盐水溶性的氧化还原引发体系，在水和二甲亚砜的混合溶剂中，可以高效地实现油溶性单体VAc的表面引发接枝聚合。经过醇解反应，成功制得表面接枝有功能大分子PVA的功能接枝微粒PVA/SiO2。
然后，以接枝微粒PVA/SiO2为固体吸附剂，对槲皮素、芦丁和染料木素三种黄酮类化合物进行了吸附研究，考察了主要因素对吸附性能的影响，深入探讨了吸附机理，并研究了吸附热力学。研究结果表明，由于接枝微粒PVA/SiO2表面含有高密度的羟基，使得该接枝微粒与黄酮类化合物分子之间，可形成多位点的常规氢键和π型氢键，正是这两种类型的氢键使功能接枝微粒PVA/SiO_2对三种黄酮都产生了很强的物理吸附作用。溶剂的竞争吸附对黄酮化合物的吸附容量会产生很大的影响，以弱极性的1,2-二氯乙烷(DCE)为溶剂时，几乎不存在溶剂的竞争吸附，槲皮素、芦丁和染料木素具有很高的吸附容量，而在质子溶剂乙醇中，强烈的溶剂竞争吸附使三者的吸附容量均大大降低。升高温度会减弱氢键作用，甚至使氢键断裂，导致吸附容量减小。质子溶剂中电解质的存在，对吸附作用产生负性影响。功能微粒PVA/SiO_2对黄酮类化合物的吸附为放热过程，且为焓驱动的吸附过程，吸附模式符合Langmuir模型。
最后，使用本课题组新建立的“接枝交联聚合与印迹过程同步进行”的新型分子表面印迹方法，以甲基丙烯酸(MAA)为功能单体，染料木素为模板分子，凭借主-客体之间的常规氢键和π型氢键两类氢键相互作用，在氨基/过硫酸盐氧化还原引发体系作用下，使MAA及交联剂N,N'-亚甲基双丙烯酰胺(MBA)在硅胶微粒表面发生接枝交联聚合，除去模板，成功地制备了染料木素分子表面印迹材料MIP-PMAA/SiO_2。采用静态、动态及竞争性吸附等实验方法，考察了染料木素印迹材料MIP-PMAA/SiO_2对染料木素的识别选择性与结合亲和性，研究结果表明，染料木素表面印迹材料对模板分子具有优良的结合亲和性，结合容量可高达0.36mmol·g~(-1)，更重要的是该印迹材料对染料木素具有特异的识别选择性，相对于槲皮素和芦丁，MIP-PMAA/SiO_2对染料木素的选择性系数分别高达5.4和11.8。研究结果还表明，印迹条件对印迹材料的选择性能具有很大的影响，当单体与模板的比例为4:1，单体与交联剂的比例为5:1时，印迹材料具有最好的识别选择性。
依同样的方法，制备槲皮素分子表面印迹材料MIP-PMAA/SiO_2，并考察槲皮素印迹材料对槲皮素的识别选择性和结合亲和性。研究结果表明，该印迹材料对槲皮素具有高的结合性能和识别选择性能。结合容量达0.33mmol·g~(-1)；相对于染料木素和芦丁，印迹材料对槲皮素的选择性系数分别为4.4和7.69。此外，槲皮素印迹材料还具有优良的洗脱性能，以0.001mmol·L~(-1)的NaOH作为洗脱剂，在19个床体积槲皮素的解吸率高达98.1%，有利于印迹材料的再生和重复使用。
Flavonoids are a broad class of natural substance that exist extensively in plant tissuesand have various biological activities. Modern pharmacological studies indicate that theyhave multiple functions of free-radical scavenging, antioxidation, antitumor, sterilization,and so on. So they play an important role in natural medicine chemistry. Quercetin, rutin,genistein are three typical flavonoid compounds which have high bioactivity andpharmacological activities. Therefore, effective extraction and separation of them fromplants have attracted much attention in the chemistry and biomedical field. In this study, inorder to promote the development of extraction and separation of natural medicinecompounds, adsorbing materials which have strong adsorption properties to flavonoids areprepared，and flavonoids surface molecular imprinted polymers (MIPs) are also preparedby using a new surface molecular imprinting technique “grafting polymerizationsynchronized with imprinting” by means of molecular designing. So this research has greatscientific significance in biopharmaceutics and chemical sciences fields.
First, micron-sized silica gel particles are surface-modified with coupling agentγ-Aminopropyltrimethoxysilane (AMPS), obtaining the modified particles AMPS-SiO_2, onwhich primary amino groups are chemically attached. Distilled water and DMSO are usedas mixed solvents, and a redox initiation system is constituted with the amino groups onthe surfaces of AMPS-SiO_2particles and ammonium persulphate in a solution. Thispractice realizes the surface-initiated graft-polymerization of vinyl acetate (VAc), andprepares the grafted particles PVAc/SiO_2with a high grafting degree of PVAc. PVAc canbe transformed into PVA by the alcoholysis reaction, which results in functional graftedparticles PVA/SiO_2with high grafting degree. The product particles are characterized byFTIR, SEM and TGA. The effects of main factors on the graft-polymerization of vinyl acetate are investigated emphatically. The experimental results show that the mixedsolvents composed of water and DMSO along with a redox initiation system constitutedwith the amino groups on the surfaces of AMPS-SiO_2particles and ammonium persulphatein a solution realize highly effective surface-initiated graft-polymerization of vinyl acetatewhich is a oil-soluble monomer. Then the functional material PVA/SiO_2is successfullyobtained through the alcoholysis reaction of PVAc.
Subsequently, the adsorption investigation of three kinds of flavonoids, quercetin,rutin and genistein was conducted by using polyvinyl alcohol (PVA)-grafted silica gelparticles PVA/SiO_2as solid adsorbent. The effects of the main factors on the adsorptionproperties are investigated, the adsorption mechanism is explored in depth, and theadsorption thermodynamic is researched. The experimental results show that theconventional hydrogen bond is formed between the hydroxyl groups with high density onthe surfaces of PVA/SiO_2and the phenolic hydroxyl groups in flavonoids, while π-typehydrogen bond is formed between the hydroxyl groups of PVA/SiO_2and the conjugatedaromatic rings. It is the two types of hydrogen bond that make the functional compositeparticles PVA/SiO_2produce very strong physical adsorption toward quercetin, rutin andgenistein. The competitive adsorption of the solvent can have severe negative impact onthe adsorption capacity of flavonoids. In1,2-dichloroethane (DCE) as a weak polaritysolvent, the competing adsorption of the solvent barely exists. Therefore, when DCE isused as solvent, quercetin, rutin and genistein have very high adsorption capacities. Whilein ethanol as a protic solvent, the adsorption capacities of the three flavonoids declinegreatly as a result of the strong competing adsorption of the solvent. Increasingtemperature will weaken the hydrogen bond interaction between PVA/SiO_2particles andflavonoids, and even make the hydrogen bond rupture, leading to the decrease of theadsorption capacity. The existence of electrolytes in the protic solvent will affect theadsorption negatively. The adsorption process of PVA/SiO_2particles toward flavonoids isexothermic and driven by enthalpy. The adsorption isotherm data matches the Langmuirmodel.
Lastly, by using the newly established surface molecular imprinting technique“grafting polymerization synchronized with imprinting”, genistein molecularly imprintedpolymer MIP-PMAA/SiO_2is successfully prepared. The main procedures are as follows.(1)In DMF solvent, monomer MAA gathers around template genistein with two types ofhydrogen-bond interaction.(2) A redox surface-initiated system is constituted betweenprimary amine group and the ammonium persulfate.(3) Monomer MAA and crosslinkerMBA carry out grafting/crosslinking copolymerization.(4) The template genistein isremoved, obtaining genistein molecular imprinted polymers MIP-PMAA/SiO_2. Theadsorption and recognition characteristics of MIP-PMAA/SiO_2for genistein are studied indepth by using static method, dynamic method and competitive adsorption experiment. Theexperimental results show that MIP-PMAA/SiO_2possesses very strong adsorption affinityfor genistein, and the saturated adsorption capacity could reach to0.36mmol·g~(-1). Moreimportantly, MIP-PMAA/SiO_2possesses excellent specific recognition to genistein. Theselectivity coefficients relative to quercetin and rutin are5.4and11.8, respectively. Besides,the imprinting condition has a great influence on selective recognition properties ofgenistein MIPs. When the ratio of monomer MAA and genistein is4:1, monomer MAAand crosslinker MBA is5:1, MIP-PMAA/SiO_2has greatest selective recognitioncharacteristics.
A similar method can be adopted to prepare quercetin molecularly imprinted polymerMIP-PMAA/SiO_2, and study its adsorption and recognition characteristics. Theexperimental results show that MIP-PMAA/SiO_2possesses very strong adsorption affinityand specific recognition for quercetin. And the saturated adsorption capacity could reach to0.33mmol·g~(-1). The selectivity coefficients relative to genistein and rutin are4.4and7.69,respectively. Besides, elution experiment is performed by using sodium hydroxide solutionwith a concentration of0.001mol·L~(-1)(pH value of10) as eluting agent, within19bedvolumes, quercetin is eluted from MIP-PMAA/SiO_2column with a desorption ratio of98.1%. That way, the new adsorbent MIP-PMAA/SiO_2has outstanding elution propertyand it exhibits excellent reusability.
首先，使用偶联剂γ-氨丙基三甲氧基硅烷(AMPS)，对微米级硅胶微粒进行了化学改性，制得表面带有伯胺基的改性微粒AMPS-SiO2，使用水和二甲亚砜的混合溶剂，使改性微粒AMPS-SiO2表面的氨基与溶液中的过硫酸铵构成氧化-还原引发体系，实现了乙酸乙烯酯(VAc)在硅胶微粒表面的引发接枝聚合，制得了高接枝度的接枝微粒PVAc/SiO2。经醇解，将PVAc转变为聚乙烯醇(PVA)，从而制备了高接枝度的功能接枝微粒PVA/SiO2。采用红外光谱(FTIR)、扫描电镜(SEM)及热重分析(TGA)等方法对产物微粒进行了表征。并深入研究了主要因素对VAc表面引发接枝聚合的影响。研究结果表明，采用氨基与过硫酸盐水溶性的氧化还原引发体系，在水和二甲亚砜的混合溶剂中，可以高效地实现油溶性单体VAc的表面引发接枝聚合。经过醇解反应，成功制得表面接枝有功能大分子PVA的功能接枝微粒PVA/SiO2。
然后，以接枝微粒PVA/SiO2为固体吸附剂，对槲皮素、芦丁和染料木素三种黄酮类化合物进行了吸附研究，考察了主要因素对吸附性能的影响，深入探讨了吸附机理，并研究了吸附热力学。研究结果表明，由于接枝微粒PVA/SiO2表面含有高密度的羟基，使得该接枝微粒与黄酮类化合物分子之间，可形成多位点的常规氢键和π型氢键，正是这两种类型的氢键使功能接枝微粒PVA/SiO_2对三种黄酮都产生了很强的物理吸附作用。溶剂的竞争吸附对黄酮化合物的吸附容量会产生很大的影响，以弱极性的1,2-二氯乙烷(DCE)为溶剂时，几乎不存在溶剂的竞争吸附，槲皮素、芦丁和染料木素具有很高的吸附容量，而在质子溶剂乙醇中，强烈的溶剂竞争吸附使三者的吸附容量均大大降低。升高温度会减弱氢键作用，甚至使氢键断裂，导致吸附容量减小。质子溶剂中电解质的存在，对吸附作用产生负性影响。功能微粒PVA/SiO_2对黄酮类化合物的吸附为放热过程，且为焓驱动的吸附过程，吸附模式符合Langmuir模型。
最后，使用本课题组新建立的“接枝交联聚合与印迹过程同步进行”的新型分子表面印迹方法，以甲基丙烯酸(MAA)为功能单体，染料木素为模板分子，凭借主-客体之间的常规氢键和π型氢键两类氢键相互作用，在氨基/过硫酸盐氧化还原引发体系作用下，使MAA及交联剂N,N'-亚甲基双丙烯酰胺(MBA)在硅胶微粒表面发生接枝交联聚合，除去模板，成功地制备了染料木素分子表面印迹材料MIP-PMAA/SiO_2。采用静态、动态及竞争性吸附等实验方法，考察了染料木素印迹材料MIP-PMAA/SiO_2对染料木素的识别选择性与结合亲和性，研究结果表明，染料木素表面印迹材料对模板分子具有优良的结合亲和性，结合容量可高达0.36mmol·g~(-1)，更重要的是该印迹材料对染料木素具有特异的识别选择性，相对于槲皮素和芦丁，MIP-PMAA/SiO_2对染料木素的选择性系数分别高达5.4和11.8。研究结果还表明，印迹条件对印迹材料的选择性能具有很大的影响，当单体与模板的比例为4:1，单体与交联剂的比例为5:1时，印迹材料具有最好的识别选择性。
依同样的方法，制备槲皮素分子表面印迹材料MIP-PMAA/SiO_2，并考察槲皮素印迹材料对槲皮素的识别选择性和结合亲和性。研究结果表明，该印迹材料对槲皮素具有高的结合性能和识别选择性能。结合容量达0.33mmol·g~(-1)；相对于染料木素和芦丁，印迹材料对槲皮素的选择性系数分别为4.4和7.69。此外，槲皮素印迹材料还具有优良的洗脱性能，以0.001mmol·L~(-1)的NaOH作为洗脱剂，在19个床体积槲皮素的解吸率高达98.1%，有利于印迹材料的再生和重复使用。
Flavonoids are a broad class of natural substance that exist extensively in plant tissuesand have various biological activities. Modern pharmacological studies indicate that theyhave multiple functions of free-radical scavenging, antioxidation, antitumor, sterilization,and so on. So they play an important role in natural medicine chemistry. Quercetin, rutin,genistein are three typical flavonoid compounds which have high bioactivity andpharmacological activities. Therefore, effective extraction and separation of them fromplants have attracted much attention in the chemistry and biomedical field. In this study, inorder to promote the development of extraction and separation of natural medicinecompounds, adsorbing materials which have strong adsorption properties to flavonoids areprepared，and flavonoids surface molecular imprinted polymers (MIPs) are also preparedby using a new surface molecular imprinting technique “grafting polymerizationsynchronized with imprinting” by means of molecular designing. So this research has greatscientific significance in biopharmaceutics and chemical sciences fields.
First, micron-sized silica gel particles are surface-modified with coupling agentγ-Aminopropyltrimethoxysilane (AMPS), obtaining the modified particles AMPS-SiO_2, onwhich primary amino groups are chemically attached. Distilled water and DMSO are usedas mixed solvents, and a redox initiation system is constituted with the amino groups onthe surfaces of AMPS-SiO_2particles and ammonium persulphate in a solution. Thispractice realizes the surface-initiated graft-polymerization of vinyl acetate (VAc), andprepares the grafted particles PVAc/SiO_2with a high grafting degree of PVAc. PVAc canbe transformed into PVA by the alcoholysis reaction, which results in functional graftedparticles PVA/SiO_2with high grafting degree. The product particles are characterized byFTIR, SEM and TGA. The effects of main factors on the graft-polymerization of vinyl acetate are investigated emphatically. The experimental results show that the mixedsolvents composed of water and DMSO along with a redox initiation system constitutedwith the amino groups on the surfaces of AMPS-SiO_2particles and ammonium persulphatein a solution realize highly effective surface-initiated graft-polymerization of vinyl acetatewhich is a oil-soluble monomer. Then the functional material PVA/SiO_2is successfullyobtained through the alcoholysis reaction of PVAc.
Subsequently, the adsorption investigation of three kinds of flavonoids, quercetin,rutin and genistein was conducted by using polyvinyl alcohol (PVA)-grafted silica gelparticles PVA/SiO_2as solid adsorbent. The effects of the main factors on the adsorptionproperties are investigated, the adsorption mechanism is explored in depth, and theadsorption thermodynamic is researched. The experimental results show that theconventional hydrogen bond is formed between the hydroxyl groups with high density onthe surfaces of PVA/SiO_2and the phenolic hydroxyl groups in flavonoids, while π-typehydrogen bond is formed between the hydroxyl groups of PVA/SiO_2and the conjugatedaromatic rings. It is the two types of hydrogen bond that make the functional compositeparticles PVA/SiO_2produce very strong physical adsorption toward quercetin, rutin andgenistein. The competitive adsorption of the solvent can have severe negative impact onthe adsorption capacity of flavonoids. In1,2-dichloroethane (DCE) as a weak polaritysolvent, the competing adsorption of the solvent barely exists. Therefore, when DCE isused as solvent, quercetin, rutin and genistein have very high adsorption capacities. Whilein ethanol as a protic solvent, the adsorption capacities of the three flavonoids declinegreatly as a result of the strong competing adsorption of the solvent. Increasingtemperature will weaken the hydrogen bond interaction between PVA/SiO_2particles andflavonoids, and even make the hydrogen bond rupture, leading to the decrease of theadsorption capacity. The existence of electrolytes in the protic solvent will affect theadsorption negatively. The adsorption process of PVA/SiO_2particles toward flavonoids isexothermic and driven by enthalpy. The adsorption isotherm data matches the Langmuirmodel.
Lastly, by using the newly established surface molecular imprinting technique“grafting polymerization synchronized with imprinting”, genistein molecularly imprintedpolymer MIP-PMAA/SiO_2is successfully prepared. The main procedures are as follows.(1)In DMF solvent, monomer MAA gathers around template genistein with two types ofhydrogen-bond interaction.(2) A redox surface-initiated system is constituted betweenprimary amine group and the ammonium persulfate.(3) Monomer MAA and crosslinkerMBA carry out grafting/crosslinking copolymerization.(4) The template genistein isremoved, obtaining genistein molecular imprinted polymers MIP-PMAA/SiO_2. Theadsorption and recognition characteristics of MIP-PMAA/SiO_2for genistein are studied indepth by using static method, dynamic method and competitive adsorption experiment. Theexperimental results show that MIP-PMAA/SiO_2possesses very strong adsorption affinityfor genistein, and the saturated adsorption capacity could reach to0.36mmol·g~(-1). Moreimportantly, MIP-PMAA/SiO_2possesses excellent specific recognition to genistein. Theselectivity coefficients relative to quercetin and rutin are5.4and11.8, respectively. Besides,the imprinting condition has a great influence on selective recognition properties ofgenistein MIPs. When the ratio of monomer MAA and genistein is4:1, monomer MAAand crosslinker MBA is5:1, MIP-PMAA/SiO_2has greatest selective recognitioncharacteristics.
A similar method can be adopted to prepare quercetin molecularly imprinted polymerMIP-PMAA/SiO_2, and study its adsorption and recognition characteristics. Theexperimental results show that MIP-PMAA/SiO_2possesses very strong adsorption affinityand specific recognition for quercetin. And the saturated adsorption capacity could reach to0.33mmol·g~(-1). The selectivity coefficients relative to genistein and rutin are4.4and7.69,respectively. Besides, elution experiment is performed by using sodium hydroxide solutionwith a concentration of0.001mol·L~(-1)(pH value of10) as eluting agent, within19bedvolumes, quercetin is eluted from MIP-PMAA/SiO_2column with a desorption ratio of98.1%. That way, the new adsorbent MIP-PMAA/SiO_2has outstanding elution propertyand it exhibits excellent reusability.
引文
[1]裴凌鹏,惠伯棣,金宗濂,等.黄酮类化合物的生理活性及其制备技术研究进展[J].食品科学,2004,25(2):203-207.
[2] Choi H J, Song J H, Park K S, et al. Inhibitory effects of quercetin3-rhamnoside oninfluenza A virus replication[J]. European Journal of Pharmaceutical Sciences,2009,37(3):329-333.
[3] Sánchez-Gallego J I, López-Revuelta A, Hernández-Hernández A, et al. Comparativeantioxidant capacities of quercetin and butylated hydroxyanisole incholesterol-modified erythrocytes damaged by tert-butylhydroperoxide[J]. Food andChemical Toxicology,2011,49(9):2212-2221.
[4] Rusznyak S, Szent-Gy rgyi A. Vitamin P: flavonols as vitamins[J]. Nature,1936,138(3479):27.
[5] Robards K, Antolovich M. Analytical Chemistry of Fruit BioflavonoidsA Review[J].Analyst,1997,122(2):11R-34R.
[6]秦震.黄酮分子印迹硅胶微球的制备及其分子识别机理的研[D].天津大学,2007.
[7]薛昆鹏.油茶果壳中活性成分纯化新方法及其在绿色合成中的应用[D].南昌航空大学,2012.
[8] Packer L, Rimbach G, Virgili F. Antioxidant activity and biologic properties of aprocyanidin-rich extract from pine (pinus maritima) bark, pycnogenol[J]. Free RadicalBiology and Medicine,1999,27(5):704-724.
[9] Shirley B W. Flavonoid biosynthesis:‘new’functions for an‘old’pathway[J]. Trends inPlant Science,1996,1(11):377-382.
[10] Verpoorte R, Memelink J. Engineering secondary metabolite production in plants[J].Current Opinion in Biotechnology,2002,13(2):181-187.
[11]朱丹,袁芳,孟坤,等.黄酮类化合物的研究进展[J].中华中医药杂志,2007,22(6):387-389.
[12]张德权,台建祥.生物类黄酮的研究及应用概况[J].食品与发酵工业,1999,25(6):52-57.
[13]王倩,常丽新,唐红梅.黄酮类化合物的提取分离及其生物活性研究进展[J].河北理工大学学报:自然科学版,2011,33(1):110-115.
[14] Urquiaga I, Leighton F. Plant polyphenol antioxidants and oxidative stress[J].Biological Research,2000,33(2):55-64.
[15] Garcia-Salas P, Morales-Soto A, Segura-Carretero A, et al. Phenolic-compound-extraction systems for fruit and vegetable samples[J]. Molecules,2010,15(12):8813-8826.
[16]王玮,王琳.黄酮类化合物的研究进展[J].沈阳医学院学报,2002,4(2):115-119.
[17]陈晓慧,徐雅琴.黄酮类化合物生物活性及在食品中的应用[J].食品工程,2006,(3):12-14.
[18] Pietta P G. Flavonoids as antioxidants[J]. Journal of Natural Products,2000,63(7):1035-1042.
[19] Kandaswami C, Middleton Jr E. Free radical scavenging and antioxidant activity ofplant flavonoids[M]. Free Radicals in Diagnostic Medicine.1994,366:351-376.
[20] Afanas' ev I B, Dcrozhko A I, Brodskii A V, et al. Chelating and free radicalscavenging mechanisms of inhibitory action of rutin and quercetin in lipidperoxidation[J]. Biochemical Pharmacology,1989,38(11):1763-1769.
[21]孟庆华.天然黄酮类化合物清除自由基机理及其应用进展[J].云南民族大学学报:自然科学版,2012,21(2):79-83.
[22] Skaltsa H, Verykokidou E, Harvala C, et al. UV-B protective potential and flavonoidcontent of leaf hairs of Quercus ilex[J]. Phytochemistry,1994,37(4):987-990.
[23]盛幼珍,王丽梅,余龙江,等.几种天然植物黄酮的抗氧化对比研究[J].食品科技,2008,(7):176-178.
[24] Lopez-Lazaro M. Flavonoids as anticancer agents: structure-activity relationshipstudy[J]. Current Medicinal Chemistry-Anti-Cancer Agents,2002,2(6):691-714.
[25] Kanadaswami C, Lee L-T, Lee P-P H, et al. The antitumor activities of flavonoids[J].In Vivo,2005,19(5):895-909.
[26] Liu H L, Jiang W B, Xie M X. Flavonoids: recent advances as anticancer drugs[J].Recent Patents on Anti-Cancer Drug Discovery,2010,5(2):152-164.
[27] Clere N, Faure S, Carmen Martinez M, et al. Anticancer properties of flavonoids: rolesin various stages of carcinogenesis[J]. Cardiovascular&Hematological Agents inMedicinal Chemistry,2011,9(2):62-77.
[28] Huber A, Thongphasuk P, Erben G, et al. Significantly greater antioxidant anticanceractivities of2,3-dehydrosilybin than silybin[J]. Biochimica et Biophysica Acta(BBA)-General Subjects,2008,1780(5):837-847.
[29] Handayani R, Rice L, Cui Y, et al. Soy isoflavones alter expression of genesassociated with cancer progression, including interleukin-8, in androgen-independentPC-3human prostate cancer cells[J]. The Journal of Nutrition,2006,136(1):75-82.
[30] Terao J, Kawai Y, Murota K. Vegetable flavonoids and cardiovascular disease[J]. AsiaPacific Journal of Clinical Nutrition,2008,17(1):291-293.
[31]何惟胜.葛根及其提取物治疗心脑血管疾病药理和临床研究[J].时珍国医国药,2001,12(5):470-471.
[32] Gryglewski R J, Korbut R, Robak J, et al. On the mechanism of antithrombotic actionof flavonoids[J]. Biochemical Pharmacology,1987,36(3):317-322.
[33] Tripoli E, Guardia M L, Giammanco S, et al. Citrus flavonoids: Molecular structure,biological activity and nutritional properties: A review[J]. Food Chemistry,2007,104(2):466-479.
[34]张锦,高尔.银杏黄酮磷脂复合物对大鼠心肌再灌注血管内皮损伤的保护作用[J].数理医药学杂志,2009,22(1):15-18.
[35]宋雪鹏,陈平平,柴象枢.葛根素对自发高血压大鼠的降压作用及对其血浆肾素活性的影响[J].中国药理学报,1998,9(1):55-55.
[36]罗超,宣页沁.黄酮类化合物治疗糖尿病研究进展[J].国际内分泌代谢杂志,2013,33(5):339-341.
[37] Song Y, Manson J A E, Buring J E, et al. Associations of dietary flavonoids with riskof type2diabetes, and markers of insulin resistance and systemic inflammation inwomen: a prospective study and cross-sectional analysis[J]. Journal of the AmericanCollege of Nutrition,2005,24(5):376-384.
[38] Zand R S R, Jenkins D J A, Diamandis E P. Steroid hormone activity of flavonoidsand related compounds[J]. Breast Cancer Research and Treatment,2000,62(1):35-49.
[39] Toh M F, Sohn J, Chen S N, et al. Biological characterization of non-steroidalprogestins from botanicals used for women’s health[J]. Steroids,2012,77(7):765-773.
[40] Yamaguchi M. The osteogenic effect of bioactive flavonoid p-hydroxycinnamic acid:development in osteoporosis treatment[J]. OA Biotechnology,2013,2(2):1-8.
[41] Songlin P, Ge Z, Yixin H, et al. Epimedium-derived flavonoids promoteosteoblastogenesis and suppress adipogenesis in bone marrow stromal cells whileexerting an anabolic effect on osteoporotic bone[J]. Bone,2009,45(3):534-544.
[42] Puel C, Mathey J, Kati-Coulibaly S, et al. Preventive effect of Abelmoschus manihot(L.) Medik. on bone loss in the ovariectomised rats[J]. Journal of Ethnopharmacology,2005,99(1):55-60.
[43]刘平,陈光晖,邓淑华,等.金莲花总黄酮抗菌作用的实验研[J].中国实验方剂学杂志,2013,19(6):207-210.
[44]张生潭,王兆玉,兰新宇,等.响应面法优化麻疯树叶总黄酮提取工艺及其抗菌活性研究[J].中药材,2013,36(2):308-311.
[45] Cushnie T P, Lamb A J. Recent advances in understanding the antibacterial propertiesof flavonoids[J]. International Journal of Antimicrobial Agents,2011,38(2):99-107.
[46]杨艳,彭璇,朱籎,等.荔枝核黄酮类化合物的体外抗腺病毒[J].武汉大学学报:医学版,2014,35(1):41-45.
[47]赵铁华,邓淑华,杨鹤松,等.半枝莲总黄酮抗甲型H1N1流感病毒感染的药效学研究[J].中国药理学通报,2014,30(1):147-148.
[48] Orhan D D, z elik B, zgen S, et al. Antibacterial, antifungal, and antiviral activitiesof some flavonoids[J]. Microbiological Research,2010,165(6):496-504.
[49]何可,陈兴颖,宛燕飞,等.仙人掌总黄酮抗炎作用的研究[J].中国兽医科学,2011,41(10):1065-1069.
[50]郎玉英,张琦.紫苏总黄酮的抗炎作用研究[J].中草药,2010(5):791-794.
[51] Kawai M, Hirano T, Higa S, et al. Flavonoids and related compounds as anti-allergicsubstances[J]. Allergol Int,2007,56(2):113-123.
[52] Seelinger G, Merfort I, Schempp C M. Anti-oxidant, anti-inflammatory andanti-allergic activities of luteolin[J]. Planta Medica,2008,74(14):1667-1677.
[53] Nkengfack A E, Vouffo T W, Fomum Z T, et al. Prenylated isoflavanone from theroots of Erythrina sigmoidea[J]. Phytochemistry,1994,36(4):1047-1051.
[54] Aquila S, Giner R M, Recio M C, et al. Anti-inflammatory activity of flavonoids fromCayaponia tayuya roots[J]. Journal of Ethnopharmacology,2009,121(2):333-337.
[55]崔山风.槲皮素的研究进展[J].西北药学杂志,2006,21(6):279-281.
[56]许进军,何东初.槲皮素研究进展[J].实用预防医学,2006,13(4):1095-1097.
[57]孙涓,余世春.槲皮素的研究进展[J].现代中药研究与实践,2011,25(3):85-88.
[58]韩英华,秦元璋.芦丁研究现状[J].山东中医杂志,2003,22(10):635-637.
[59]龙全江,杨韬.芦丁的研究概况及展望[J].中国中医药信息杂志,2002,9(4):39-42.
[60]张萍,郑万金,仲英.染料木素的研究进展[J].齐鲁药事,2008,27(2):103-106.
[61] Baghel S S, Shrivastava N, Baghel R S, et al. A review of quercetin: antioxidant andanticancer properties[J]. World Journal of Pharmacy and Pharmaceutical Sciences,2012,1(1):146-160.
[62] Dajas F. Life or death: neuroprotective and anticancer effects of quercetin[J]. Journalof Ethnopharmacology,2012,143(2):383-396.
[63]刘敏,肖颖,左爱仁,等.槲皮素,根皮素,水飞蓟宾清除自由基和抑制脂质过氧化活性研究[J].中成药,2012,34(4):753-756.
[64] Zielińska M, Kostrzewa A, Ignatowicz E. Antioxidative activity of flavonoids instimulated human neutrophils[J]. Folia Histochemica et Cytobiologica/PolishAcademy of Sciences, Polish Histochemical and Cytochemical Society,2000,38(1):25-30.
[65] Rodgers E H, Grant M H. The effect of the flavonoids, quercetin, myricetin andepicatechin on the growth and enzyme activities of MCF7human breast cancercells[J]. Chemico-Biological Interactions,1998,116(3):213-228.
[66] Castrillo J L, Carrasco L. Action of3-methylquercetin on poliovirus RNAreplication[J]. Journal of Virology,1987,61(10):3319-3321.
[67]李厚丽,翟光喜，马玉坤，等．均匀设计优化制备槲皮素固体脂质纳米粒[J]．食品与药品，2000，9(2)：78-81．
[68]翟光喜，娄红祥，邹立家，等．药物磷脂复合物的研究进展[J].中国药学杂志，2001,36(12)：800-803．
[69]孙震，邱明丰,李绍顾，等．水飞蓟素漓丸的溶出度研究[J].中国中药杂志,2005,30(4)：263-265．
[70] Chua L S. A review on plant-based rutin extraction methods and its pharmacologicalactivities[J]. Journal of Ethnopharmacology,2013,150(3):805-817.
[71] Jiménez-Aliaga K, Bermejo-Bescós P, Benedí J, et al. Quercetin and rutin exhibitantiamyloidogenic and fibril-disaggregating effects in vitro and potent antioxidantactivity in APPswe cells[J]. Life Sciences,2011,89(25):939-945.
[72]田建坤,赵永和,时文中.芦丁及其衍生物应用研究进展[J].天中学刊,2007,22(5):18-21.
[73] Banerjee S, Zhang Y, Ali S, et al. Molecular evidence for increased antitumor activityof gemcitabine by genistein in vitro and in vivo using an orthotopic model ofpancreatic cancer[J]. Cancer Research,2005,65(19):9064-9072.
[74] Vantyghem S A, Wilson S M, Postenka C O, et al. Dietary genistein reducesmetastasis in a postsurgical orthotopic breast cancer model[J]. Cancer Research,2005,65(8):3396-3403.
[75] Wei H, Ca Q, Rahn R, et al. DNA structural integrity and base composition affectultraviolet light-induced oxidative DNA damage[J]. Biochemistry,1998,37(18):6485-6490.
[76] Qi L H, Kang L P, Zhang J P, et al. Antifibrotic effects of genistein and quercetin invitro[J]. Acta Pharmaceutica Sinica,2001,36(9):648-651.
[77] Jinrong L, Hui X, Wenyan L, et al. Synthesis, Pharmacological Activity of a Series ofGenistein Derivatives as Modulators of Multidrug Resistance[J]. Chinese Journal ofOrganic Chemistry,2011,31(11):1884-1892.
[78]余立华,刘康,刘保林.染料木素的药理作用[J].药学与临床研究,2010,18(3):299-302.
[79]丁利君,吴振辉.菊花中黄酮类物质提取方法的研究[J].食品工业科技,2002,23(2):20-22.
[80]曹永刚.芦丁提取工艺的研究[J].中国医药工业杂志,1993,24(2):51-52.
[81]金时,杨梅,孔羽,等.木豆叶中黄酮微波提取工艺研究[J].中草药,2011,42(11):2235-2239.
[82] Routray W, Orsat V. Microwave-assisted extraction of flavonoids: a review[J]. Foodand Bioprocess Technology,2012,5(2):409-424.
[83] Xiao W, Han L, Shi B. Microwave-assisted extraction of flavonoids from RadixAstragali[J]. Separation and Purification Technology,2008,62(3):614-618.
[84]蒙松年,陈代武.微波辅助提取槐米中的槲皮素[J].华西药学杂志,2006,20(6):561-562.
[85] Chen L, Ding L, Yu A, et al. Continuous determination of total flavonoids inPlatycladus orientalis (L.) Franco by dynamic microwave-assisted extraction coupledwith on-line derivatization and ultraviolet–visible detection[J]. Analytica ChimicaActa,2007,596(1):164-170.
[86] Zhang G, He L, Hu M. Optimized ultrasonic-assisted extraction of flavonoids fromPrunella vulgaris L. and evaluation of antioxidant activities in vitro[J]. InnovativeFood Science&Emerging Technologies,2011,12(1):18-25.
[87] Wu Q, Jia J. Study on Ultrasonic-assisted Extraction and Antioxidation of PomeloPeel Flavonoids [J]. Food Science,2009,30(2):29-33.
[88]仇燕,仇彦博.柚皮总黄酮超声波辅助提取工艺的研究[J].河北科技大学学报,2010,31(4):330-333.
[89]马国刚.槐米芦丁的超声波辅助提取及化学修饰研究[D].北京林业大学硕士学位论文,2008.
[90]徐萌萌,沈竞,徐春,等.槐米固态发酵提高槲皮素含量的研究[J].时珍国医国药,2008,19(3):704-706.
[91]陈源,潘东明,郭金鹏,等.酶法辅助提取金柑皮总黄酮的工艺优化研究[J].中国农学通报,2011,27(7):442-446.
[92] Yan J, Chen J. Study on the extract of flavonoids from the green persimmon fruit dropby enzyme hydrolysis method[J]. Science and Technology of Food Industry,2011,6:081.
[93]陈佳.洋葱皮黄酮酶法辅助提取,纯化及体外抗氧化研究[D].西北农林科技大学,2011.
[94] Wijesinghe W, Jeon Y J. Enzyme-assistant extraction (EAE) of bioactive components:A useful approach for recovery of industrially important metabolites from seaweeds:A review[J]. Fitoterapia,2012,83(1):6-12.
[95] Liza M S, Abdul Rahman R, Mandana B, et al. Supercritical carbon dioxide extractionof bioactive flavonoid from Strobilanthes crispus (Pecah Kaca)[J]. Food andBioproducts Processing,2010,88(2):319-326.
[96] Miao S F, Yu J P, Du Z, et al. Supercritical fluid extraction and micronization ofginkgo flavonoids from ginkgo biloba leaves[J]. Industrial&Engineering ChemistryResearch,2010,49(11):5461-5466.
[97]孙佳石,董金香,邱智东.超临界CO2流体对槐花中槲皮素提取的工艺研究[A].中华中医药学会第九届制剂学术研讨会论文汇编,2008.
[98] Yang C, Xu Y R, Yao W X. Extraction of pharmaceutical components from Ginkgobiloba leaves using supercritical carbon dioxide[J]. Journal of Agricultural and FoodChemistry,2002,50(4):846-849.
[99]杨光义,叶方,王刚,等.半仿生提取法在中药新药研究中的应用[J].中国药师,2010(8):1188-1190.
[100]张俊龙,郭蕾,李钦青,等.半仿生提取法应用的研究进展[J].山西中医学院学报,2013,14(4):73-75.
[101]庞中磊,唐文.柚皮总黄酮半仿生提取工艺研究[J].上海应用技术学院学报:自然科学版,2012,12(1):6-8.
[102]李敏,马晓.槐米中芦丁的微波提取研究[J].今日科苑,2009(6):44-44.
[103]许政,赵志强.柱层析技术在中药材有效成分分离纯化中的应用[J].中国中医药信息杂志,2013,20(12):109-110.
[104]穆青，赵南勋，王丽斐，等．大豆异黄酮的分离与纯化[J].天然产物研究与开发,2006,18(Suppl);118-121.
[105]甘春丽,王晶,杨异卉,等.聚酰胺柱色谱法分离黄酮醇与二氢黄酮醇类化合物[J].哈尔滨医科大学学报,2008,41(6):552-554.
[106]李教社,赵玉英.密蒙花黄酮类化合物的分离和鉴定[J].药学学报,1996,31(11):849-854.
[107] Shi S, Huang K, Zhang Y, et al. Preparative isolation and purification of twoflavonoid glycosides from Taraxacum mongolicum by high-speed counter-currentchromatography[J]. Separation and Purification Technology,2008,60(1):81-85.
[108]邸多隆,郑媛嫒,陈小芬,等.高速逆流色谱技术分离纯化天然产物中黄酮类化合物的研究进展[J].分析化学,2011,39(2):269-275.
[109] Deng S, Deng Z, Fan Y, et al. Isolation and purification of three flavonoid glycosidesfrom the leaves of Nelumbo nucifera (Lotus) by high-speed counter-currentchromatography[J]. Journal of Chromatography B,2009,877(24):2487-2492.
[110]曲丽萍,宓鹤呜,范国荣,等.高速逆流色谱法分离制备淡豆豉中大豆素和染料木素[J].中草药,2006,37(3):375-377.
[111] Gaggeri R, Rossi D, Collina S, et al. Quick development of an analyticalenantioselective high performance liquid chromatography separation and preparativescale-up for the flavonoid Naringenin[J]. Journal of Chromatography A,2011,1218(32):5414-5422.
[112] Zhang Y, Jiao J, Liu C, et al. Isolation and purification of four flavone C-glycosidesfrom antioxidant of bamboo leaves by macroporous resin column chromatographyand preparative high-performance liquid chromatography[J]. Food Chemistry,2008,107(3):1326-1336.
[113]孙亚男,李彤,马辰.高效液相色谱手性流动相添加剂法分离3种黄酮类化合物对映体[J].色谱,2013,31(5):447-450.
[114]余丽娟,陈全斌,义祥辉,等.高效液相色谱法制备罗汉果甜甙标准品[J].色谱,2003,21(4):397-399.
[115]徐龙泉,彭黔荣,杨敏,等.膜分离技术在中药生产及研究中的应用进[J].中成药,2013,35(9):1989-1994.
[116]陈玲,李炳辉,张志平,等.紫花杜鹃干燥嫩叶中黄酮类化合物的提取及膜分离纯化[J].食品科学,2009(24):34-37.
[117]于涛,钱和.膜分离技术在提取银杏叶黄酮类化合物中的应用[J].无锡轻工大学学报,2004,23(6):55-58.
[118] Wang Y, Liu L. Using ultrafiltration membrane technologies purified licoriceflavonoids [J]. Chemical Research and Application,2012,24(4):646-649.
[119]张旭,王锦玉,仝燕,等.大孔树脂技术在中药提取纯化中的应用及展[J].中国实验方剂学杂志,2012,18(6):286-290.
[120] Lou S, Chen Z, Liu Y, et al. Synthesis of functional adsorption resin and itsadsorption properties in purification of flavonoids from Hippophae rhamnoides L.leaves[J]. Industrial&Engineering Chemistry Research,2012,51(6):2682-2696.
[121]冯希勇.槐米中芦丁提取工艺研究[J].内蒙古中医药,2008,18:54-54.
[122]田冠峰,徐明雅,杨亦文,等. D301阴离子交换树脂对大豆异黄酮分离特性的影响[J].食品与发酵工业,2006,32(6):133-136.
[123] Yu J S, Yu J P. Separation and purification of flavonoids from chrysanthemumindicum with macroporous resin[J]. China Journal of Chinese Materia Medica,2007,32(20):2123-2127.
[124]张琦弦,李娟,张文华,等.胶原纤维吸附剂分离芦丁和槲皮素[J].生物质化学工程,2012,46(1):1-5.
[125]赵利剑,杨亚玲,夏静.固相萃取技术的研究[J].四川化工,2005,8(3):21-25.
[126]李存法,何金环.固相萃取技术及其应用[J].天中学刊,2005,20(5):13-16.
[127] Tamami B, Allahyari H, Ghasemi S, et al. Palladium nanoparticles supported on poly(N-vinylpyrrolidone)-grafted silica as new recyclable catalyst for Heck cross-couplingreactions[J]. Journal of Organometallic Chemistry,2011,696(2):594-599.
[128] Shao D, Xu K, Song X, et al. Effective adsorption and separation of lysozyme withPAA-modified Fe3O4@silica core/shell microspheres[J]. Journal of Colloid andInterface Science,2009,336(2):526-532.
[129] Anirudhan T S, Rejeena S R, Tharun A R. Preparation, characterization andadsorption behavior of tannin-modified poly(glycidylmethacrylate)-grafted zirconiumoxide-densified cellulose for the selective separation of bovine serum albumin[J].Colloids and Surfaces B: Biointerfaces,2012,93:49-58.
[130]王海燕,曾艳丽,孟令鹏,等.有关氢键理论研究的现状及前景[J].河北师范大学学报(自然科学版),2005,29(2):177-181.
[131] Geng X, Ren P, Pi G, et al. High selective purification of flavonoids from naturalplants based on polymeric adsorbent with hydrogen-bonding interaction[J]. Journal ofChromatography A,2009,1216(47):8331-8338.
[132]刘睿,王芃,施荣富,等.氢键吸附树脂的合成及高纯度沙棘叶黄酮的制备[J].高分子学报,2010,1(10):1211-1217.
[133] Zhang Q, Li J, Zhang W, et al. Adsorption Chromatography Separation of Baicaleinand Baicalin Using Collagen Fiber Adsorbent[J]. Industrial&Engineering ChemistryResearch,2013,52(6):2425-2433.
[134] Kajjout M, Zemmouri R, Rolando C. An expeditious synthesis of quercetin3-O-β-d-glucuronide from rutin[J]. Tetrahedron Letters,2011,52(37):4738-4740.
[135] Tasselli F, Donato L, Drioli E. Evaluation of molecularly imprinted membranesbased on different acrylic copolymers[J]. Journal of Membrane Science,2008,320(1):167-172.
[136] Zhang Y, Gao B. Preparation of Functional Grafted Particles PVA/SiO2with HighGrafting Degree and Preliminary Research of Their Adsorption Character[J]. Journalof Macromolecular Science, Part A,2013,50(2):238-247.
[137]张妍妍,高保娇,门吉英.聚乙烯醇接枝微粒对黄酮类物质的吸附特性[J].高等学校化学学报,2013,34(4):1000-1006.
[138] Zhang Y, Gao B, Xu Z. Adsorption Properties of Polyvinyl-Alcohol-Grafted ParticlesToward Genistein Driven by Hydrogen-Bond Interaction[J]. The Journal of PhysicalChemistry B,2013,117(18):5730-5736.
[139] Claude B, Morin P, Lafosse M, et al. Selective solid-phase extraction of a triterpeneacid from a plant extract by molecularly imprinted polymer[J]. Talanta,2008,75(2):344-350.
[140] Dias A C B, Figueiredo E C, Grassi V, et al. Molecularly imprinted polymer as asolid phase extractor in flow analysis[J]. Talanta,2008,76(5):988-996.
[141] Shi Y, Zhang J H, Shi D, et al. Selective solid-phase extraction of cholesterol usingmolecularly imprinted polymers and its application in different biological samples[J].Journal of Pharmaceutical and Biomedical Analysis,2006,42(5):549-555.
[142] Wang Y, Zhou Y, Sokolov J, et al. A potentiometric protein sensor built with surfacemolecular imprinting method[J]. Biosensors and Bioelectronics,2008,24(1):162-166.
[143] Liu H, Liu C, Yang X, et al. Uniformly sized β-cyclodextrin molecularly imprintedmicrospheres prepared by a novel surface imprinting technique for ursolic acid[J].Analytica Chimica Acta,2008,628(1):87-94.
[144] Haijia S, Ying Z, Jia L, et al. Biosorption of Ni2+by the surface molecular imprintingadsorbent[J]. Process Biochemistry,2006,41(6):1422-1426.
[145] Kunitake T, Lee S W. Molecular imprinting in ultrathin titania gel films via surfacesol–gel process[J]. Analytica Chimica Acta,2004,504(1):1-6.
[146] An F, Gao B. Adsorption characteristics of Cr (III) ionic imprinting polyamine onsilica gel surface[J]. Desalination,2009,249(3):1390-1396.
[147] An F, Gao B, Feng X. Adsorption of2,4,6-trinitrotoluene on a novel adsorptionmaterial PEI/SiO2[J]. Journal of Hazardous Materials,2009,166(2):757-761.
[148] Zhang Y, Gao B, Xu Z. Adsorption and recognition characteristics of surfaceimprinted material MIP-PMAA/SiO2toward genistein [J]. Journal of ChromatographyA.(Accepted)
[149] Euterpio M A, Pagano I, Piccinelli A L, et al. Development and Validation of aMethod for the Determination of (E)-Resveratrol and Related Phenolic Compounds inBeverages Using Molecularly Imprinted Solid Phase Extraction[J]. Journal ofAgricultural and Food Chemistry,2012,61(8):1640-1645.
[150] Song X, Li J, Wang J, et al. Quercetin molecularly imprinted polymers: preparation,recognition characteristics and properties as sorbent for solid-phase extraction[J].Talanta,2009,80(2):694-702.
[151]杨安平.印迹聚合物的制备、表征及对黄酮的吸附[D].中南民族大学,2007.
[1] Tang E, Cheng G, Ma X. Preparation of nano-ZnO/PMMA composite particles viagrafting of the copolymer onto the surface of zinc oxide nanoparticles[J]. PowderTechnology,2006,161(3):209-214.
[2] Ngo V G, Bressy C, Leroux C, et al. Synthesis of hybrid TiO2nanoparticles withwell-defined poly (methyl methacrylate) and poly (tert-butyldimethylsilylmethacrylate) via the RAFT process[J]. Polymer,2009,50(14):3095-3102.
[3] Chinthamanipeta P S, Kobukata S, Nakata H, et al. Synthesis of poly (methylmethacrylate)-silica nanocomposites using methacrylate-functionalized silicananoparticles and RAFT polymerization[J]. Polymer,2008,49(26):5636-5642.
[4] Sideridou I D, Karabela M M. Effect of the amount of3-methacyloxypropyl-trimethoxysilane coupling agent on physical properties of dental resinnanocomposites[J]. Dental Materials,2009,25(11):1315-1324.
[5] Demirel G B, aykara T. DNA immobilization on polymer-modified Si surface bycontrolling pH[J]. Applied Surface Science,2009,255(13):6571-6576.
[6] Bialk M, Prucker O, Rühe J. Grafting of polymers to solid surfaces by usingimmobilized methacrylates[J]. Colloids and Surfaces A: Physicochemical andEngineering Aspects,2002,198:543-549.
[7] Tsubokawa N, Hayashi S, Nishimura J. Grafting of hyperbranched polymers ontoultrafine silica: postgraft polymerization of vinyl monomers initiated by pendant azogroups of grafted polymer chains on the surface[J]. Progress in Organic Coatings,2002,44(1):69-74.
[8]卢金华,高保娇.粒径可控的聚乙烯醇交联微球VA/DVB的制备[J].功能高分子学报,2008,21(3):284-290.
[1] Poplewska I, Pi tkowski W, Antos D. Effect of temperature on competitive adsorptionof the solute and the organic solvent in reversed-phase liquid chromatography[J].Journal of Chromatography A,2006,1103(2):284-295.
[2] Kumagai S, Ishizawa H, Toida Y. Influence of solvent type on dibenzothiopheneadsorption onto activated carbon fiber and granular coconut-shell activated carbon[J].Fuel,2010,89(2):365-371.
[3] Esumi K, Ishizuki K, Otsuka H, et al. The effect of binary solvents on adsorption ofpoly (vinylpyrrolidone) on titanium dioxide and graphite particles[J]. Journal ofColloid and Interface Science,1996,178(2):549-554.
[4] Czeslik C, Jonas J. Pressure and temperature dependence of hydrogen-bond strength inmethanol clusters[J]. Chemical Physics Letters,1999,302(5):633-638.
[5] Ohtaki H. Effects of temperature and pressure on hydrogen bonds in water and informamide[J]. Journal of Molecular Liquids,2003,103:3-13.
[6] Ross P D, Rekharsky M V. Thermodynamics of hydrogen bond and hydrophobicinteractions in cyclodextrin complexes[J]. Biophysical Journal,1996,71(4):2144-2154.
[7] Jose T M, Aneesh M H, Kannan M P. Cation-π interaction in potassium–polyenecomplexes and the fate of potassium ion: A theoretical study[J]. Journal of MolecularGraphics and Modelling,2007,26(4):783-787.
[8] Escudero D, Frontera A, Qui onero D, et al. Interplay between cation-π and hydrogenbonding interactions[J]. Chemical Physics Letters,2008,456(4):257-261.
[9] Gromiha M M, Suwa M. Structural analysis of residues involving cation-π interactionsin different folding types of membrane proteins[J]. International Journal of BiologicalMacromolecules,2005,35(1):55-62.
[10] Cestari A R, Vieira E F S, Mattos C R S. Thermodynamics of the Cu (II) adsorption onthin vanillin-modified chitosan membranes[J]. The Journal of ChemicalThermodynamics,2006,38(9):1092-1099.
[11] dos Anjos F S C, Vieira E F S, Cestari A R. Interaction of indigo carmine dye withchitosan evaluated by adsorption and thermochemical data[J]. Journal of Colloid andInterface Science,2002,253(2):243-246.
[1] Poplewska I, Pi tkowski W, Antos D. Effect of temperature on competitive adsorptionof the solute and the organic solvent in reversed-phase liquid chromatography[J].Journal of Chromatography A,2006,1103(2):284-295.
[2] Kumagai S, Ishizawa H, Toida Y. Influence of solvent type on dibenzothiopheneadsorption onto activated carbon fiber and granular coconut-shell activated carbon[J].Fuel,2010,89(2):365-371.
[3] Esumi K, Ishizuki K, Otsuka H, et al. The effect of binary solvents on adsorption ofpoly (vinylpyrrolidone) on titanium dioxide and graphite particles[J]. Journal ofcolloid and interface science,1996,178(2):549-554.
[4] Czeslik C, Jonas J. Pressure and temperature dependence of hydrogen-bond strength inmethanol clusters[J]. Chemical physics letters,1999,302(5):633-638.
[5] Ohtaki H. Effects of temperature and pressure on hydrogen bonds in water and informamide[J]. Journal of molecular liquids,2003,103:3-13.
[6] Ross P D, Rekharsky M V. Thermodynamics of hydrogen bond and hydrophobicinteractions in cyclodextrin complexes[J]. Biophysical journal,1996,71(4):2144-2154.
[7] Cestari A R, Vieira E F S, Mattos C R S. Thermodynamics of the Cu (II) adsorption onthin vanillin-modified chitosan membranes[J]. The Journal of ChemicalThermodynamics,2006,38(9):1092-1099.
[8] dos Anjos F S C, Vieira E F S, Cestari A R. Interaction of indigo carmine dye withchitosan evaluated by adsorption and thermochemical data[J]. Journal of colloid andinterface science,2002,253(2):243-246.
[9]谢祖芳,夏金虹,晏全.聚酰胺吸附硝基苯酚的热力学与机理探讨[J].化工技术与开发,2003,32(2):10-13.
[1]李丁,高保娇,位霄鹏.表面引发接枝聚合法制备高接枝度的接枝微粒SiO2-g-PMAA[J].应用化学,2011,28(2):154-158.
[2] Ers z A, Say R, Denizli A. Ni (II) ion-imprinted solid-phase extraction andpreconcentration in aqueous solutions by packed-bed columns[J]. Analytica ChimicaActa,2004,502(1):91-97.
[3] Say R, Ers z A, Türk H, et al. Selective separation and preconcentration of cyanide bya column packed with cyanide-imprinted polymeric microbeads[J]. Separation andPurification Technology,2004,40(1):9-14.
[4]杜俊玫,高保娇,黄小卫,等.基于离子交换和表面引发接枝聚合制备阴离子表面印迹材料及其识别特性研究[J].化学学报,2012,70:1831-1838.
[5]程伟,高保娇,施雪军,等.表面引发接枝聚合法制备抗蚜威分子表面印迹材料及其分子识别与结合特性研究[J].高分子学报,2013,(7):934-942.
[1] Ers z A, Say R, Denizli A. Ni (II) ion-imprinted solid-phase extraction andpreconcentration in aqueous solutions by packed-bed columns[J]. Analytica ChimicaActa,2004,502(1):91-97.
[2] Say R, Ers z A, Türk H, et al. Selective separation and preconcentration of cyanide bya column packed with cyanide-imprinted polymeric microbeads[J]. Separation andPurification Technology,2004,40(1):9-14.
[3]杜俊玫,高保娇,黄小卫,等.基于离子交换和表面引发接枝聚合制备阴离子表面印迹材料及其识别特性研究[J].化学学报,2012,70:1831-1838.
[4]程伟,高保娇,施雪军,等.表面引发接枝聚合法制备抗蚜威分子表面印迹材料及其分子识别与结合特性研究[J].高分子学报,2013(7):934-942.
[2] Choi H J, Song J H, Park K S, et al. Inhibitory effects of quercetin3-rhamnoside oninfluenza A virus replication[J]. European Journal of Pharmaceutical Sciences,2009,37(3):329-333.
[3] Sánchez-Gallego J I, López-Revuelta A, Hernández-Hernández A, et al. Comparativeantioxidant capacities of quercetin and butylated hydroxyanisole incholesterol-modified erythrocytes damaged by tert-butylhydroperoxide[J]. Food andChemical Toxicology,2011,49(9):2212-2221.
[4] Rusznyak S, Szent-Gy rgyi A. Vitamin P: flavonols as vitamins[J]. Nature,1936,138(3479):27.
[5] Robards K, Antolovich M. Analytical Chemistry of Fruit BioflavonoidsA Review[J].Analyst,1997,122(2):11R-34R.
[6]秦震.黄酮分子印迹硅胶微球的制备及其分子识别机理的研[D].天津大学,2007.
[7]薛昆鹏.油茶果壳中活性成分纯化新方法及其在绿色合成中的应用[D].南昌航空大学,2012.
[8] Packer L, Rimbach G, Virgili F. Antioxidant activity and biologic properties of aprocyanidin-rich extract from pine (pinus maritima) bark, pycnogenol[J]. Free RadicalBiology and Medicine,1999,27(5):704-724.
[9] Shirley B W. Flavonoid biosynthesis:‘new’functions for an‘old’pathway[J]. Trends inPlant Science,1996,1(11):377-382.
[10] Verpoorte R, Memelink J. Engineering secondary metabolite production in plants[J].Current Opinion in Biotechnology,2002,13(2):181-187.
[11]朱丹,袁芳,孟坤,等.黄酮类化合物的研究进展[J].中华中医药杂志,2007,22(6):387-389.
[12]张德权,台建祥.生物类黄酮的研究及应用概况[J].食品与发酵工业,1999,25(6):52-57.
[13]王倩,常丽新,唐红梅.黄酮类化合物的提取分离及其生物活性研究进展[J].河北理工大学学报:自然科学版,2011,33(1):110-115.
[14] Urquiaga I, Leighton F. Plant polyphenol antioxidants and oxidative stress[J].Biological Research,2000,33(2):55-64.
[15] Garcia-Salas P, Morales-Soto A, Segura-Carretero A, et al. Phenolic-compound-extraction systems for fruit and vegetable samples[J]. Molecules,2010,15(12):8813-8826.
[16]王玮,王琳.黄酮类化合物的研究进展[J].沈阳医学院学报,2002,4(2):115-119.
[17]陈晓慧,徐雅琴.黄酮类化合物生物活性及在食品中的应用[J].食品工程,2006,(3):12-14.
[18] Pietta P G. Flavonoids as antioxidants[J]. Journal of Natural Products,2000,63(7):1035-1042.
[19] Kandaswami C, Middleton Jr E. Free radical scavenging and antioxidant activity ofplant flavonoids[M]. Free Radicals in Diagnostic Medicine.1994,366:351-376.
[20] Afanas' ev I B, Dcrozhko A I, Brodskii A V, et al. Chelating and free radicalscavenging mechanisms of inhibitory action of rutin and quercetin in lipidperoxidation[J]. Biochemical Pharmacology,1989,38(11):1763-1769.
[21]孟庆华.天然黄酮类化合物清除自由基机理及其应用进展[J].云南民族大学学报:自然科学版,2012,21(2):79-83.
[22] Skaltsa H, Verykokidou E, Harvala C, et al. UV-B protective potential and flavonoidcontent of leaf hairs of Quercus ilex[J]. Phytochemistry,1994,37(4):987-990.
[23]盛幼珍,王丽梅,余龙江,等.几种天然植物黄酮的抗氧化对比研究[J].食品科技,2008,(7):176-178.
[24] Lopez-Lazaro M. Flavonoids as anticancer agents: structure-activity relationshipstudy[J]. Current Medicinal Chemistry-Anti-Cancer Agents,2002,2(6):691-714.
[25] Kanadaswami C, Lee L-T, Lee P-P H, et al. The antitumor activities of flavonoids[J].In Vivo,2005,19(5):895-909.
[26] Liu H L, Jiang W B, Xie M X. Flavonoids: recent advances as anticancer drugs[J].Recent Patents on Anti-Cancer Drug Discovery,2010,5(2):152-164.
[27] Clere N, Faure S, Carmen Martinez M, et al. Anticancer properties of flavonoids: rolesin various stages of carcinogenesis[J]. Cardiovascular&Hematological Agents inMedicinal Chemistry,2011,9(2):62-77.
[28] Huber A, Thongphasuk P, Erben G, et al. Significantly greater antioxidant anticanceractivities of2,3-dehydrosilybin than silybin[J]. Biochimica et Biophysica Acta(BBA)-General Subjects,2008,1780(5):837-847.
[29] Handayani R, Rice L, Cui Y, et al. Soy isoflavones alter expression of genesassociated with cancer progression, including interleukin-8, in androgen-independentPC-3human prostate cancer cells[J]. The Journal of Nutrition,2006,136(1):75-82.
[30] Terao J, Kawai Y, Murota K. Vegetable flavonoids and cardiovascular disease[J]. AsiaPacific Journal of Clinical Nutrition,2008,17(1):291-293.
[31]何惟胜.葛根及其提取物治疗心脑血管疾病药理和临床研究[J].时珍国医国药,2001,12(5):470-471.
[32] Gryglewski R J, Korbut R, Robak J, et al. On the mechanism of antithrombotic actionof flavonoids[J]. Biochemical Pharmacology,1987,36(3):317-322.
[33] Tripoli E, Guardia M L, Giammanco S, et al. Citrus flavonoids: Molecular structure,biological activity and nutritional properties: A review[J]. Food Chemistry,2007,104(2):466-479.
[34]张锦,高尔.银杏黄酮磷脂复合物对大鼠心肌再灌注血管内皮损伤的保护作用[J].数理医药学杂志,2009,22(1):15-18.
[35]宋雪鹏,陈平平,柴象枢.葛根素对自发高血压大鼠的降压作用及对其血浆肾素活性的影响[J].中国药理学报,1998,9(1):55-55.
[36]罗超,宣页沁.黄酮类化合物治疗糖尿病研究进展[J].国际内分泌代谢杂志,2013,33(5):339-341.
[37] Song Y, Manson J A E, Buring J E, et al. Associations of dietary flavonoids with riskof type2diabetes, and markers of insulin resistance and systemic inflammation inwomen: a prospective study and cross-sectional analysis[J]. Journal of the AmericanCollege of Nutrition,2005,24(5):376-384.
[38] Zand R S R, Jenkins D J A, Diamandis E P. Steroid hormone activity of flavonoidsand related compounds[J]. Breast Cancer Research and Treatment,2000,62(1):35-49.
[39] Toh M F, Sohn J, Chen S N, et al. Biological characterization of non-steroidalprogestins from botanicals used for women’s health[J]. Steroids,2012,77(7):765-773.
[40] Yamaguchi M. The osteogenic effect of bioactive flavonoid p-hydroxycinnamic acid:development in osteoporosis treatment[J]. OA Biotechnology,2013,2(2):1-8.
[41] Songlin P, Ge Z, Yixin H, et al. Epimedium-derived flavonoids promoteosteoblastogenesis and suppress adipogenesis in bone marrow stromal cells whileexerting an anabolic effect on osteoporotic bone[J]. Bone,2009,45(3):534-544.
[42] Puel C, Mathey J, Kati-Coulibaly S, et al. Preventive effect of Abelmoschus manihot(L.) Medik. on bone loss in the ovariectomised rats[J]. Journal of Ethnopharmacology,2005,99(1):55-60.
[43]刘平,陈光晖,邓淑华,等.金莲花总黄酮抗菌作用的实验研[J].中国实验方剂学杂志,2013,19(6):207-210.
[44]张生潭,王兆玉,兰新宇,等.响应面法优化麻疯树叶总黄酮提取工艺及其抗菌活性研究[J].中药材,2013,36(2):308-311.
[45] Cushnie T P, Lamb A J. Recent advances in understanding the antibacterial propertiesof flavonoids[J]. International Journal of Antimicrobial Agents,2011,38(2):99-107.
[46]杨艳,彭璇,朱籎,等.荔枝核黄酮类化合物的体外抗腺病毒[J].武汉大学学报:医学版,2014,35(1):41-45.
[47]赵铁华,邓淑华,杨鹤松,等.半枝莲总黄酮抗甲型H1N1流感病毒感染的药效学研究[J].中国药理学通报,2014,30(1):147-148.
[48] Orhan D D, z elik B, zgen S, et al. Antibacterial, antifungal, and antiviral activitiesof some flavonoids[J]. Microbiological Research,2010,165(6):496-504.
[49]何可,陈兴颖,宛燕飞,等.仙人掌总黄酮抗炎作用的研究[J].中国兽医科学,2011,41(10):1065-1069.
[50]郎玉英,张琦.紫苏总黄酮的抗炎作用研究[J].中草药,2010(5):791-794.
[51] Kawai M, Hirano T, Higa S, et al. Flavonoids and related compounds as anti-allergicsubstances[J]. Allergol Int,2007,56(2):113-123.
[52] Seelinger G, Merfort I, Schempp C M. Anti-oxidant, anti-inflammatory andanti-allergic activities of luteolin[J]. Planta Medica,2008,74(14):1667-1677.
[53] Nkengfack A E, Vouffo T W, Fomum Z T, et al. Prenylated isoflavanone from theroots of Erythrina sigmoidea[J]. Phytochemistry,1994,36(4):1047-1051.
[54] Aquila S, Giner R M, Recio M C, et al. Anti-inflammatory activity of flavonoids fromCayaponia tayuya roots[J]. Journal of Ethnopharmacology,2009,121(2):333-337.
[55]崔山风.槲皮素的研究进展[J].西北药学杂志,2006,21(6):279-281.
[56]许进军,何东初.槲皮素研究进展[J].实用预防医学,2006,13(4):1095-1097.
[57]孙涓,余世春.槲皮素的研究进展[J].现代中药研究与实践,2011,25(3):85-88.
[58]韩英华,秦元璋.芦丁研究现状[J].山东中医杂志,2003,22(10):635-637.
[59]龙全江,杨韬.芦丁的研究概况及展望[J].中国中医药信息杂志,2002,9(4):39-42.
[60]张萍,郑万金,仲英.染料木素的研究进展[J].齐鲁药事,2008,27(2):103-106.
[61] Baghel S S, Shrivastava N, Baghel R S, et al. A review of quercetin: antioxidant andanticancer properties[J]. World Journal of Pharmacy and Pharmaceutical Sciences,2012,1(1):146-160.
[62] Dajas F. Life or death: neuroprotective and anticancer effects of quercetin[J]. Journalof Ethnopharmacology,2012,143(2):383-396.
[63]刘敏,肖颖,左爱仁,等.槲皮素,根皮素,水飞蓟宾清除自由基和抑制脂质过氧化活性研究[J].中成药,2012,34(4):753-756.
[64] Zielińska M, Kostrzewa A, Ignatowicz E. Antioxidative activity of flavonoids instimulated human neutrophils[J]. Folia Histochemica et Cytobiologica/PolishAcademy of Sciences, Polish Histochemical and Cytochemical Society,2000,38(1):25-30.
[65] Rodgers E H, Grant M H. The effect of the flavonoids, quercetin, myricetin andepicatechin on the growth and enzyme activities of MCF7human breast cancercells[J]. Chemico-Biological Interactions,1998,116(3):213-228.
[66] Castrillo J L, Carrasco L. Action of3-methylquercetin on poliovirus RNAreplication[J]. Journal of Virology,1987,61(10):3319-3321.
[67]李厚丽,翟光喜，马玉坤，等．均匀设计优化制备槲皮素固体脂质纳米粒[J]．食品与药品，2000，9(2)：78-81．
[68]翟光喜，娄红祥，邹立家，等．药物磷脂复合物的研究进展[J].中国药学杂志，2001,36(12)：800-803．
[69]孙震，邱明丰,李绍顾，等．水飞蓟素漓丸的溶出度研究[J].中国中药杂志,2005,30(4)：263-265．
[70] Chua L S. A review on plant-based rutin extraction methods and its pharmacologicalactivities[J]. Journal of Ethnopharmacology,2013,150(3):805-817.
[71] Jiménez-Aliaga K, Bermejo-Bescós P, Benedí J, et al. Quercetin and rutin exhibitantiamyloidogenic and fibril-disaggregating effects in vitro and potent antioxidantactivity in APPswe cells[J]. Life Sciences,2011,89(25):939-945.
[72]田建坤,赵永和,时文中.芦丁及其衍生物应用研究进展[J].天中学刊,2007,22(5):18-21.
[73] Banerjee S, Zhang Y, Ali S, et al. Molecular evidence for increased antitumor activityof gemcitabine by genistein in vitro and in vivo using an orthotopic model ofpancreatic cancer[J]. Cancer Research,2005,65(19):9064-9072.
[74] Vantyghem S A, Wilson S M, Postenka C O, et al. Dietary genistein reducesmetastasis in a postsurgical orthotopic breast cancer model[J]. Cancer Research,2005,65(8):3396-3403.
[75] Wei H, Ca Q, Rahn R, et al. DNA structural integrity and base composition affectultraviolet light-induced oxidative DNA damage[J]. Biochemistry,1998,37(18):6485-6490.
[76] Qi L H, Kang L P, Zhang J P, et al. Antifibrotic effects of genistein and quercetin invitro[J]. Acta Pharmaceutica Sinica,2001,36(9):648-651.
[77] Jinrong L, Hui X, Wenyan L, et al. Synthesis, Pharmacological Activity of a Series ofGenistein Derivatives as Modulators of Multidrug Resistance[J]. Chinese Journal ofOrganic Chemistry,2011,31(11):1884-1892.
[78]余立华,刘康,刘保林.染料木素的药理作用[J].药学与临床研究,2010,18(3):299-302.
[79]丁利君,吴振辉.菊花中黄酮类物质提取方法的研究[J].食品工业科技,2002,23(2):20-22.
[80]曹永刚.芦丁提取工艺的研究[J].中国医药工业杂志,1993,24(2):51-52.
[81]金时,杨梅,孔羽,等.木豆叶中黄酮微波提取工艺研究[J].中草药,2011,42(11):2235-2239.
[82] Routray W, Orsat V. Microwave-assisted extraction of flavonoids: a review[J]. Foodand Bioprocess Technology,2012,5(2):409-424.
[83] Xiao W, Han L, Shi B. Microwave-assisted extraction of flavonoids from RadixAstragali[J]. Separation and Purification Technology,2008,62(3):614-618.
[84]蒙松年,陈代武.微波辅助提取槐米中的槲皮素[J].华西药学杂志,2006,20(6):561-562.
[85] Chen L, Ding L, Yu A, et al. Continuous determination of total flavonoids inPlatycladus orientalis (L.) Franco by dynamic microwave-assisted extraction coupledwith on-line derivatization and ultraviolet–visible detection[J]. Analytica ChimicaActa,2007,596(1):164-170.
[86] Zhang G, He L, Hu M. Optimized ultrasonic-assisted extraction of flavonoids fromPrunella vulgaris L. and evaluation of antioxidant activities in vitro[J]. InnovativeFood Science&Emerging Technologies,2011,12(1):18-25.
[87] Wu Q, Jia J. Study on Ultrasonic-assisted Extraction and Antioxidation of PomeloPeel Flavonoids [J]. Food Science,2009,30(2):29-33.
[88]仇燕,仇彦博.柚皮总黄酮超声波辅助提取工艺的研究[J].河北科技大学学报,2010,31(4):330-333.
[89]马国刚.槐米芦丁的超声波辅助提取及化学修饰研究[D].北京林业大学硕士学位论文,2008.
[90]徐萌萌,沈竞,徐春,等.槐米固态发酵提高槲皮素含量的研究[J].时珍国医国药,2008,19(3):704-706.
[91]陈源,潘东明,郭金鹏,等.酶法辅助提取金柑皮总黄酮的工艺优化研究[J].中国农学通报,2011,27(7):442-446.
[92] Yan J, Chen J. Study on the extract of flavonoids from the green persimmon fruit dropby enzyme hydrolysis method[J]. Science and Technology of Food Industry,2011,6:081.
[93]陈佳.洋葱皮黄酮酶法辅助提取,纯化及体外抗氧化研究[D].西北农林科技大学,2011.
[94] Wijesinghe W, Jeon Y J. Enzyme-assistant extraction (EAE) of bioactive components:A useful approach for recovery of industrially important metabolites from seaweeds:A review[J]. Fitoterapia,2012,83(1):6-12.
[95] Liza M S, Abdul Rahman R, Mandana B, et al. Supercritical carbon dioxide extractionof bioactive flavonoid from Strobilanthes crispus (Pecah Kaca)[J]. Food andBioproducts Processing,2010,88(2):319-326.
[96] Miao S F, Yu J P, Du Z, et al. Supercritical fluid extraction and micronization ofginkgo flavonoids from ginkgo biloba leaves[J]. Industrial&Engineering ChemistryResearch,2010,49(11):5461-5466.
[97]孙佳石,董金香,邱智东.超临界CO2流体对槐花中槲皮素提取的工艺研究[A].中华中医药学会第九届制剂学术研讨会论文汇编,2008.
[98] Yang C, Xu Y R, Yao W X. Extraction of pharmaceutical components from Ginkgobiloba leaves using supercritical carbon dioxide[J]. Journal of Agricultural and FoodChemistry,2002,50(4):846-849.
[99]杨光义,叶方,王刚,等.半仿生提取法在中药新药研究中的应用[J].中国药师,2010(8):1188-1190.
[100]张俊龙,郭蕾,李钦青,等.半仿生提取法应用的研究进展[J].山西中医学院学报,2013,14(4):73-75.
[101]庞中磊,唐文.柚皮总黄酮半仿生提取工艺研究[J].上海应用技术学院学报:自然科学版,2012,12(1):6-8.
[102]李敏,马晓.槐米中芦丁的微波提取研究[J].今日科苑,2009(6):44-44.
[103]许政,赵志强.柱层析技术在中药材有效成分分离纯化中的应用[J].中国中医药信息杂志,2013,20(12):109-110.
[104]穆青，赵南勋，王丽斐，等．大豆异黄酮的分离与纯化[J].天然产物研究与开发,2006,18(Suppl);118-121.
[105]甘春丽,王晶,杨异卉,等.聚酰胺柱色谱法分离黄酮醇与二氢黄酮醇类化合物[J].哈尔滨医科大学学报,2008,41(6):552-554.
[106]李教社,赵玉英.密蒙花黄酮类化合物的分离和鉴定[J].药学学报,1996,31(11):849-854.
[107] Shi S, Huang K, Zhang Y, et al. Preparative isolation and purification of twoflavonoid glycosides from Taraxacum mongolicum by high-speed counter-currentchromatography[J]. Separation and Purification Technology,2008,60(1):81-85.
[108]邸多隆,郑媛嫒,陈小芬,等.高速逆流色谱技术分离纯化天然产物中黄酮类化合物的研究进展[J].分析化学,2011,39(2):269-275.
[109] Deng S, Deng Z, Fan Y, et al. Isolation and purification of three flavonoid glycosidesfrom the leaves of Nelumbo nucifera (Lotus) by high-speed counter-currentchromatography[J]. Journal of Chromatography B,2009,877(24):2487-2492.
[110]曲丽萍,宓鹤呜,范国荣,等.高速逆流色谱法分离制备淡豆豉中大豆素和染料木素[J].中草药,2006,37(3):375-377.
[111] Gaggeri R, Rossi D, Collina S, et al. Quick development of an analyticalenantioselective high performance liquid chromatography separation and preparativescale-up for the flavonoid Naringenin[J]. Journal of Chromatography A,2011,1218(32):5414-5422.
[112] Zhang Y, Jiao J, Liu C, et al. Isolation and purification of four flavone C-glycosidesfrom antioxidant of bamboo leaves by macroporous resin column chromatographyand preparative high-performance liquid chromatography[J]. Food Chemistry,2008,107(3):1326-1336.
[113]孙亚男,李彤,马辰.高效液相色谱手性流动相添加剂法分离3种黄酮类化合物对映体[J].色谱,2013,31(5):447-450.
[114]余丽娟,陈全斌,义祥辉,等.高效液相色谱法制备罗汉果甜甙标准品[J].色谱,2003,21(4):397-399.
[115]徐龙泉,彭黔荣,杨敏,等.膜分离技术在中药生产及研究中的应用进[J].中成药,2013,35(9):1989-1994.
[116]陈玲,李炳辉,张志平,等.紫花杜鹃干燥嫩叶中黄酮类化合物的提取及膜分离纯化[J].食品科学,2009(24):34-37.
[117]于涛,钱和.膜分离技术在提取银杏叶黄酮类化合物中的应用[J].无锡轻工大学学报,2004,23(6):55-58.
[118] Wang Y, Liu L. Using ultrafiltration membrane technologies purified licoriceflavonoids [J]. Chemical Research and Application,2012,24(4):646-649.
[119]张旭,王锦玉,仝燕,等.大孔树脂技术在中药提取纯化中的应用及展[J].中国实验方剂学杂志,2012,18(6):286-290.
[120] Lou S, Chen Z, Liu Y, et al. Synthesis of functional adsorption resin and itsadsorption properties in purification of flavonoids from Hippophae rhamnoides L.leaves[J]. Industrial&Engineering Chemistry Research,2012,51(6):2682-2696.
[121]冯希勇.槐米中芦丁提取工艺研究[J].内蒙古中医药,2008,18:54-54.
[122]田冠峰,徐明雅,杨亦文,等. D301阴离子交换树脂对大豆异黄酮分离特性的影响[J].食品与发酵工业,2006,32(6):133-136.
[123] Yu J S, Yu J P. Separation and purification of flavonoids from chrysanthemumindicum with macroporous resin[J]. China Journal of Chinese Materia Medica,2007,32(20):2123-2127.
[124]张琦弦,李娟,张文华,等.胶原纤维吸附剂分离芦丁和槲皮素[J].生物质化学工程,2012,46(1):1-5.
[125]赵利剑,杨亚玲,夏静.固相萃取技术的研究[J].四川化工,2005,8(3):21-25.
[126]李存法,何金环.固相萃取技术及其应用[J].天中学刊,2005,20(5):13-16.
[127] Tamami B, Allahyari H, Ghasemi S, et al. Palladium nanoparticles supported on poly(N-vinylpyrrolidone)-grafted silica as new recyclable catalyst for Heck cross-couplingreactions[J]. Journal of Organometallic Chemistry,2011,696(2):594-599.
[128] Shao D, Xu K, Song X, et al. Effective adsorption and separation of lysozyme withPAA-modified Fe3O4@silica core/shell microspheres[J]. Journal of Colloid andInterface Science,2009,336(2):526-532.
[129] Anirudhan T S, Rejeena S R, Tharun A R. Preparation, characterization andadsorption behavior of tannin-modified poly(glycidylmethacrylate)-grafted zirconiumoxide-densified cellulose for the selective separation of bovine serum albumin[J].Colloids and Surfaces B: Biointerfaces,2012,93:49-58.
[130]王海燕,曾艳丽,孟令鹏,等.有关氢键理论研究的现状及前景[J].河北师范大学学报(自然科学版),2005,29(2):177-181.
[131] Geng X, Ren P, Pi G, et al. High selective purification of flavonoids from naturalplants based on polymeric adsorbent with hydrogen-bonding interaction[J]. Journal ofChromatography A,2009,1216(47):8331-8338.
[132]刘睿,王芃,施荣富,等.氢键吸附树脂的合成及高纯度沙棘叶黄酮的制备[J].高分子学报,2010,1(10):1211-1217.
[133] Zhang Q, Li J, Zhang W, et al. Adsorption Chromatography Separation of Baicaleinand Baicalin Using Collagen Fiber Adsorbent[J]. Industrial&Engineering ChemistryResearch,2013,52(6):2425-2433.
[134] Kajjout M, Zemmouri R, Rolando C. An expeditious synthesis of quercetin3-O-β-d-glucuronide from rutin[J]. Tetrahedron Letters,2011,52(37):4738-4740.
[135] Tasselli F, Donato L, Drioli E. Evaluation of molecularly imprinted membranesbased on different acrylic copolymers[J]. Journal of Membrane Science,2008,320(1):167-172.
[136] Zhang Y, Gao B. Preparation of Functional Grafted Particles PVA/SiO2with HighGrafting Degree and Preliminary Research of Their Adsorption Character[J]. Journalof Macromolecular Science, Part A,2013,50(2):238-247.
[137]张妍妍,高保娇,门吉英.聚乙烯醇接枝微粒对黄酮类物质的吸附特性[J].高等学校化学学报,2013,34(4):1000-1006.
[138] Zhang Y, Gao B, Xu Z. Adsorption Properties of Polyvinyl-Alcohol-Grafted ParticlesToward Genistein Driven by Hydrogen-Bond Interaction[J]. The Journal of PhysicalChemistry B,2013,117(18):5730-5736.
[139] Claude B, Morin P, Lafosse M, et al. Selective solid-phase extraction of a triterpeneacid from a plant extract by molecularly imprinted polymer[J]. Talanta,2008,75(2):344-350.
[140] Dias A C B, Figueiredo E C, Grassi V, et al. Molecularly imprinted polymer as asolid phase extractor in flow analysis[J]. Talanta,2008,76(5):988-996.
[141] Shi Y, Zhang J H, Shi D, et al. Selective solid-phase extraction of cholesterol usingmolecularly imprinted polymers and its application in different biological samples[J].Journal of Pharmaceutical and Biomedical Analysis,2006,42(5):549-555.
[142] Wang Y, Zhou Y, Sokolov J, et al. A potentiometric protein sensor built with surfacemolecular imprinting method[J]. Biosensors and Bioelectronics,2008,24(1):162-166.
[143] Liu H, Liu C, Yang X, et al. Uniformly sized β-cyclodextrin molecularly imprintedmicrospheres prepared by a novel surface imprinting technique for ursolic acid[J].Analytica Chimica Acta,2008,628(1):87-94.
[144] Haijia S, Ying Z, Jia L, et al. Biosorption of Ni2+by the surface molecular imprintingadsorbent[J]. Process Biochemistry,2006,41(6):1422-1426.
[145] Kunitake T, Lee S W. Molecular imprinting in ultrathin titania gel films via surfacesol–gel process[J]. Analytica Chimica Acta,2004,504(1):1-6.
[146] An F, Gao B. Adsorption characteristics of Cr (III) ionic imprinting polyamine onsilica gel surface[J]. Desalination,2009,249(3):1390-1396.
[147] An F, Gao B, Feng X. Adsorption of2,4,6-trinitrotoluene on a novel adsorptionmaterial PEI/SiO2[J]. Journal of Hazardous Materials,2009,166(2):757-761.
[148] Zhang Y, Gao B, Xu Z. Adsorption and recognition characteristics of surfaceimprinted material MIP-PMAA/SiO2toward genistein [J]. Journal of ChromatographyA.(Accepted)
[149] Euterpio M A, Pagano I, Piccinelli A L, et al. Development and Validation of aMethod for the Determination of (E)-Resveratrol and Related Phenolic Compounds inBeverages Using Molecularly Imprinted Solid Phase Extraction[J]. Journal ofAgricultural and Food Chemistry,2012,61(8):1640-1645.
[150] Song X, Li J, Wang J, et al. Quercetin molecularly imprinted polymers: preparation,recognition characteristics and properties as sorbent for solid-phase extraction[J].Talanta,2009,80(2):694-702.
[151]杨安平.印迹聚合物的制备、表征及对黄酮的吸附[D].中南民族大学,2007.
[1] Tang E, Cheng G, Ma X. Preparation of nano-ZnO/PMMA composite particles viagrafting of the copolymer onto the surface of zinc oxide nanoparticles[J]. PowderTechnology,2006,161(3):209-214.
[2] Ngo V G, Bressy C, Leroux C, et al. Synthesis of hybrid TiO2nanoparticles withwell-defined poly (methyl methacrylate) and poly (tert-butyldimethylsilylmethacrylate) via the RAFT process[J]. Polymer,2009,50(14):3095-3102.
[3] Chinthamanipeta P S, Kobukata S, Nakata H, et al. Synthesis of poly (methylmethacrylate)-silica nanocomposites using methacrylate-functionalized silicananoparticles and RAFT polymerization[J]. Polymer,2008,49(26):5636-5642.
[4] Sideridou I D, Karabela M M. Effect of the amount of3-methacyloxypropyl-trimethoxysilane coupling agent on physical properties of dental resinnanocomposites[J]. Dental Materials,2009,25(11):1315-1324.
[5] Demirel G B, aykara T. DNA immobilization on polymer-modified Si surface bycontrolling pH[J]. Applied Surface Science,2009,255(13):6571-6576.
[6] Bialk M, Prucker O, Rühe J. Grafting of polymers to solid surfaces by usingimmobilized methacrylates[J]. Colloids and Surfaces A: Physicochemical andEngineering Aspects,2002,198:543-549.
[7] Tsubokawa N, Hayashi S, Nishimura J. Grafting of hyperbranched polymers ontoultrafine silica: postgraft polymerization of vinyl monomers initiated by pendant azogroups of grafted polymer chains on the surface[J]. Progress in Organic Coatings,2002,44(1):69-74.
[8]卢金华,高保娇.粒径可控的聚乙烯醇交联微球VA/DVB的制备[J].功能高分子学报,2008,21(3):284-290.
[1] Poplewska I, Pi tkowski W, Antos D. Effect of temperature on competitive adsorptionof the solute and the organic solvent in reversed-phase liquid chromatography[J].Journal of Chromatography A,2006,1103(2):284-295.
[2] Kumagai S, Ishizawa H, Toida Y. Influence of solvent type on dibenzothiopheneadsorption onto activated carbon fiber and granular coconut-shell activated carbon[J].Fuel,2010,89(2):365-371.
[3] Esumi K, Ishizuki K, Otsuka H, et al. The effect of binary solvents on adsorption ofpoly (vinylpyrrolidone) on titanium dioxide and graphite particles[J]. Journal ofColloid and Interface Science,1996,178(2):549-554.
[4] Czeslik C, Jonas J. Pressure and temperature dependence of hydrogen-bond strength inmethanol clusters[J]. Chemical Physics Letters,1999,302(5):633-638.
[5] Ohtaki H. Effects of temperature and pressure on hydrogen bonds in water and informamide[J]. Journal of Molecular Liquids,2003,103:3-13.
[6] Ross P D, Rekharsky M V. Thermodynamics of hydrogen bond and hydrophobicinteractions in cyclodextrin complexes[J]. Biophysical Journal,1996,71(4):2144-2154.
[7] Jose T M, Aneesh M H, Kannan M P. Cation-π interaction in potassium–polyenecomplexes and the fate of potassium ion: A theoretical study[J]. Journal of MolecularGraphics and Modelling,2007,26(4):783-787.
[8] Escudero D, Frontera A, Qui onero D, et al. Interplay between cation-π and hydrogenbonding interactions[J]. Chemical Physics Letters,2008,456(4):257-261.
[9] Gromiha M M, Suwa M. Structural analysis of residues involving cation-π interactionsin different folding types of membrane proteins[J]. International Journal of BiologicalMacromolecules,2005,35(1):55-62.
[10] Cestari A R, Vieira E F S, Mattos C R S. Thermodynamics of the Cu (II) adsorption onthin vanillin-modified chitosan membranes[J]. The Journal of ChemicalThermodynamics,2006,38(9):1092-1099.
[11] dos Anjos F S C, Vieira E F S, Cestari A R. Interaction of indigo carmine dye withchitosan evaluated by adsorption and thermochemical data[J]. Journal of Colloid andInterface Science,2002,253(2):243-246.
[1] Poplewska I, Pi tkowski W, Antos D. Effect of temperature on competitive adsorptionof the solute and the organic solvent in reversed-phase liquid chromatography[J].Journal of Chromatography A,2006,1103(2):284-295.
[2] Kumagai S, Ishizawa H, Toida Y. Influence of solvent type on dibenzothiopheneadsorption onto activated carbon fiber and granular coconut-shell activated carbon[J].Fuel,2010,89(2):365-371.
[3] Esumi K, Ishizuki K, Otsuka H, et al. The effect of binary solvents on adsorption ofpoly (vinylpyrrolidone) on titanium dioxide and graphite particles[J]. Journal ofcolloid and interface science,1996,178(2):549-554.
[4] Czeslik C, Jonas J. Pressure and temperature dependence of hydrogen-bond strength inmethanol clusters[J]. Chemical physics letters,1999,302(5):633-638.
[5] Ohtaki H. Effects of temperature and pressure on hydrogen bonds in water and informamide[J]. Journal of molecular liquids,2003,103:3-13.
[6] Ross P D, Rekharsky M V. Thermodynamics of hydrogen bond and hydrophobicinteractions in cyclodextrin complexes[J]. Biophysical journal,1996,71(4):2144-2154.
[7] Cestari A R, Vieira E F S, Mattos C R S. Thermodynamics of the Cu (II) adsorption onthin vanillin-modified chitosan membranes[J]. The Journal of ChemicalThermodynamics,2006,38(9):1092-1099.
[8] dos Anjos F S C, Vieira E F S, Cestari A R. Interaction of indigo carmine dye withchitosan evaluated by adsorption and thermochemical data[J]. Journal of colloid andinterface science,2002,253(2):243-246.
[9]谢祖芳,夏金虹,晏全.聚酰胺吸附硝基苯酚的热力学与机理探讨[J].化工技术与开发,2003,32(2):10-13.
[1]李丁,高保娇,位霄鹏.表面引发接枝聚合法制备高接枝度的接枝微粒SiO2-g-PMAA[J].应用化学,2011,28(2):154-158.
[2] Ers z A, Say R, Denizli A. Ni (II) ion-imprinted solid-phase extraction andpreconcentration in aqueous solutions by packed-bed columns[J]. Analytica ChimicaActa,2004,502(1):91-97.
[3] Say R, Ers z A, Türk H, et al. Selective separation and preconcentration of cyanide bya column packed with cyanide-imprinted polymeric microbeads[J]. Separation andPurification Technology,2004,40(1):9-14.
[4]杜俊玫,高保娇,黄小卫,等.基于离子交换和表面引发接枝聚合制备阴离子表面印迹材料及其识别特性研究[J].化学学报,2012,70:1831-1838.
[5]程伟,高保娇,施雪军,等.表面引发接枝聚合法制备抗蚜威分子表面印迹材料及其分子识别与结合特性研究[J].高分子学报,2013,(7):934-942.
[1] Ers z A, Say R, Denizli A. Ni (II) ion-imprinted solid-phase extraction andpreconcentration in aqueous solutions by packed-bed columns[J]. Analytica ChimicaActa,2004,502(1):91-97.
[2] Say R, Ers z A, Türk H, et al. Selective separation and preconcentration of cyanide bya column packed with cyanide-imprinted polymeric microbeads[J]. Separation andPurification Technology,2004,40(1):9-14.
[3]杜俊玫,高保娇,黄小卫,等.基于离子交换和表面引发接枝聚合制备阴离子表面印迹材料及其识别特性研究[J].化学学报,2012,70:1831-1838.
[4]程伟,高保娇,施雪军,等.表面引发接枝聚合法制备抗蚜威分子表面印迹材料及其分子识别与结合特性研究[J].高分子学报,2013(7):934-942.
© 2004-2018 中国地质图书馆版权所有 京ICP备05064691号 京公网安备11010802017129号 地址：北京市海淀区学院路29号 邮编：100083 电话：办公室：(+86 10)66554848；文献借阅、咨询服务、科技查新：66554700 |