农林8号m苯达松敏感致死性的遗传分析及其基因定位
|
摘要
苯达松作为一种除草剂主要用于水稻、花生等农作物的田间防除杂草。普通水稻品种具有对苯达松的抗性。1984年，日本学者Mori从农林8号诱变获得突变体—农林8号m，它对苯达松极其敏感，甚至在抽穗灌浆期的成株也能被杀死。该性状称为水稻苯达松的敏感致死性(Bentazon Susceptible Lethality，简称BSL)。农林8号m的苯达松敏感致死性状在杂交稻制种过程中具有特殊的意义。本研究以农林8号m为研究对象，对田间使用苯达松的浓度做了分析。将其分别与(六千辛B／4008B)F_5、(3726B／海八B)F_5和(3726B／南65B)F_5杂交，构建F_1、BC_1、F_2群体和F_(2：3)家系，对农林8号m的苯达松敏感致死性进行了遗传分析，并在此基础上，以(六千辛B／4008B)F_5／农林8号m的F_2群体为材料，利用SSR分子标记技术，对苯达松敏感致死基因(bsl基因)进行定位，结果如下：
1 田间苯达松的浓度实验
在苯达松有效浓度为0.1％～0.4％(V／V)范围内，农林8号m表现敏感症状，而(六千辛B／4008B)F_5、(3726B／海八B)F_5和(3726B／南65B)F_5在有效浓度0.4％的下则是安全的。田间使用有效浓度为0.2％的苯达松溶液即可进行苯达松敏感致死性状鉴定。
2 农林8号m苯达松敏感致死性的遗传分析
对农林8号m及其与(六千辛B／4008B)F_5、(3726B／海八B)F_5和(3726B／南65B)F_5构建的正反交F_1、F_2、BC_1和F_(2：3)家系进行分析。结果表明，农林8号m的苯达松敏感致死性状由一对隐性单基因控制，可在水稻杂交后代中稳定遗传。
3 农林8号m苯达松敏感致死基因(bsl基因)定位
本研究利用“(六千辛B／4008B)F_5×农林8号m”的F_2分离群体，对苯达松敏感致死基因进行了SSR分析，结果表明苯达松敏感致死基因位于水稻第3染色体上，并获得2个与苯达松敏感致死性状连锁的SSR标记，这2个标记分别为RM1350和RM3856，它们位于bsl基因的同侧，与bsl基因的遗传距离分别为15.0cM和14.1cM。
Bentazon as a herbicide has been mainly applied in crop field , such as rice, peanut and so on . Most rice varieties are resistant to bentazon. A mutant - Norm 8m , susceptible to bentazon, was obtained by radiation. Bentazon Susceptible Lethality(BSL) in Norin 8m means special significance in hybrid seed production. Concentration test was taken with Norin 8m, (liuqianxinB/4008B)F5, (3726B/HaibaB)F5 and (3726B/Nan65B)F5. By crossing this mutant with (liuqianxinB/4008B)F5 , (3726B/HaibaB)F5 and (3726B/Nan65B)F3 , F1, BC1, F2 population and F2:3 family lines were developed for genetic analysis and molecular mapping for BSL. The results as follow:
1 Concentration Test
While the concentration ranging from 0.1% - 0.4%(v/v), (liuqianxinB/4008B)F5, (3726B/HaibaB)F5 and (3726B/Nan65B)F5 could maintain normal growth, but Norin 8m was Susceptible and killed 5-7 days after spraying Bentazon. Concentration of 0.2% (v/v) was suitable for identification in the fields.
2 Genetic Analysis for BSL in Norin 8m
Genetic analysis was conducted with F1, BC1, F2 population and F2:3 family lines derived from the crosses of Norin 8m and (liuqianxinB/4008B)F5, (3726B/HaibaB)F5 and (3726B/Nan65B)F5. The results indicated that BSL in Norin 8m was controlled by a recessive gene and delivered steadily in rice offspring.
3 SSR Markers Linked to bsl gene in Norin 8m
Simple sequence repeats (SSR) was employed to detect molecular markers linked to bsl gene in Norin 8m using FI segregation population derived from the crosse of Norin 8m and (HuqianxinB/4008B)F5. RM1350 and RM3856 which were all from rice chromosome 3 were proved to link to bsl gene on the same flank with genetic distance of 15.0 cM and 14.1 cM respectively.
1 田间苯达松的浓度实验
在苯达松有效浓度为0.1％～0.4％(V／V)范围内，农林8号m表现敏感症状，而(六千辛B／4008B)F_5、(3726B／海八B)F_5和(3726B／南65B)F_5在有效浓度0.4％的下则是安全的。田间使用有效浓度为0.2％的苯达松溶液即可进行苯达松敏感致死性状鉴定。
2 农林8号m苯达松敏感致死性的遗传分析
对农林8号m及其与(六千辛B／4008B)F_5、(3726B／海八B)F_5和(3726B／南65B)F_5构建的正反交F_1、F_2、BC_1和F_(2：3)家系进行分析。结果表明，农林8号m的苯达松敏感致死性状由一对隐性单基因控制，可在水稻杂交后代中稳定遗传。
3 农林8号m苯达松敏感致死基因(bsl基因)定位
本研究利用“(六千辛B／4008B)F_5×农林8号m”的F_2分离群体，对苯达松敏感致死基因进行了SSR分析，结果表明苯达松敏感致死基因位于水稻第3染色体上，并获得2个与苯达松敏感致死性状连锁的SSR标记，这2个标记分别为RM1350和RM3856，它们位于bsl基因的同侧，与bsl基因的遗传距离分别为15.0cM和14.1cM。
Bentazon as a herbicide has been mainly applied in crop field , such as rice, peanut and so on . Most rice varieties are resistant to bentazon. A mutant - Norm 8m , susceptible to bentazon, was obtained by radiation. Bentazon Susceptible Lethality(BSL) in Norin 8m means special significance in hybrid seed production. Concentration test was taken with Norin 8m, (liuqianxinB/4008B)F5, (3726B/HaibaB)F5 and (3726B/Nan65B)F5. By crossing this mutant with (liuqianxinB/4008B)F5 , (3726B/HaibaB)F5 and (3726B/Nan65B)F3 , F1, BC1, F2 population and F2:3 family lines were developed for genetic analysis and molecular mapping for BSL. The results as follow:
1 Concentration Test
While the concentration ranging from 0.1% - 0.4%(v/v), (liuqianxinB/4008B)F5, (3726B/HaibaB)F5 and (3726B/Nan65B)F5 could maintain normal growth, but Norin 8m was Susceptible and killed 5-7 days after spraying Bentazon. Concentration of 0.2% (v/v) was suitable for identification in the fields.
2 Genetic Analysis for BSL in Norin 8m
Genetic analysis was conducted with F1, BC1, F2 population and F2:3 family lines derived from the crosses of Norin 8m and (liuqianxinB/4008B)F5, (3726B/HaibaB)F5 and (3726B/Nan65B)F5. The results indicated that BSL in Norin 8m was controlled by a recessive gene and delivered steadily in rice offspring.
3 SSR Markers Linked to bsl gene in Norin 8m
Simple sequence repeats (SSR) was employed to detect molecular markers linked to bsl gene in Norin 8m using FI segregation population derived from the crosse of Norin 8m and (HuqianxinB/4008B)F5. RM1350 and RM3856 which were all from rice chromosome 3 were proved to link to bsl gene on the same flank with genetic distance of 15.0 cM and 14.1 cM respectively.
引文
1．陈忠明，邹江石等．水稻农林8号m苯达松敏感致死性的遗传及其应用前景[J]．杂交水稻，1999，14(2)：39～40
2．[3]董凤高，朱旭东，熊振民，等 以淡绿叶为标记的籼型光-敏核不育系的选育[J]中国水稻科学，1995，9(2)：65～70
3．邓华凤，李必湖，刘友芳，等．水稻光温敏核不育系起点温度提纯的实践与探讨[J]：杂交水稻，1997(6)：7～9．
4．黄大年，李敬阳等．用抗除草剂基因快速检测和提高杂交稻纯度的新技术．科学通报，1998，43(1)：67～70
5．李秀梅，吴竞仑等．国产苯达松在移栽稻田的除草效果和安全性．江苏农业科学，1996(4)：35～37
6．廖伏明，袁隆平．水稻光温敏核不育系起点温度遗传纯化的策略探讨[J]．杂交水稻，1996(6)：1～4．
7．刘树兵，王洪刚，孔令让等．高等植物的遗传作图[J]．山东农业大学学报(自然科学版)，1999，30(1)：73～78
8．陆作楣．我国杂交稻制种的基本成就与当前的问题和建议．杂交水稻，1989年增刊，1～4．
9．陆作楣等．杂交稻混杂退化问题研究．中国农业科学，1982(3)：8～15
10．陆作楣等．论我国杂交稻亲本繁育技术的演变．杂交水稻，1993(2)：1～3
11．陆作楣等．水稻雄性不育系自交结实问题之探讨．南京农业大学学报，1989，12(2)：4～11
12．罗孝和，邱趾忠，李任华．导致不育临界温度低的两用不育系培矮 64S[J]．杂交水稻，1992(2)：27～29．
13．马文宾，庄杰云，彭应财等．三系杂交稻亲本随几扩增多态性 DNA(RAPD)分析[J]．遗传，1998，20(2)：1～4
14．牟同敏，李春海，杨国才，等 紫叶水稻苗期叶色的遗传研究[J] 中国水稻科学，1995，9(1)：45～48
15．申宗坦主编．水稻籼粳亚种间杂种优势利用研究进展．浙江省881 2攻关计划论文集．北京：中国农业科技出版社．
16．沈又佳，陆作楣．种子纯度鉴定方法的研究——“赣化二号”杂交水稻种子的芽鞘色鉴定法．种子，1985(3)：15～186
17．苏少泉主编．除草剂作用靶标与新品种创制．北京：化学工业出版社，2001
18．谭震波．近等基因系和群分法在植物基因定位中的应用．遗传，1996，18(增刊)：27～30
19．唐梅，何光华，裴炎．中籼杂交水稻亲本多态性的AFLP分析[J]．遗传，2002，24(4)：439～441
20．王镭，郑茂波．遗传标记的研究进展[J]．生物技术，2002，12(2)：41～42
21．西北农学院．作物育种学[M]．北京：农业出版社，1991．42．
22．肖层林，周承恕．两系杂交稻种子纯度的影响因素与保纯技术．杂交水稻，2000，15(2)：12～14．
23．徐云碧．分子标记在数量基因定位中的应用[J]．遗传，1992，14(2)：45～48
24．袁隆平．水稻光温敏核不育系的提纯和原种生产[J]．杂交水稻，1994(6)：1．
25．张光恒，钱前等．试论杂交水稻种子纯度分析技术及其研究进展．种子，1909(1)：31～33
26．张亚兵，孔令传，刘华开，等．野败型杂交水稻种子纯度室内幼苗鉴定方法研究．杂交水稻，1997，12(3)：16～18
27．周天理等．水稻不育系中杂株的来源及遗传分析．作物学报，1992(5)：12～14．
28．朱启升，王安东等．水稻除草剂敏感基因导入恢复系的研究．杂交水稻，2000，15(3)：5～6
29. Abenes MLP. Selection of bacterial leaf blight resistance plants in the F_2 generation via their linkage to molecular markers[J]. Rice Genet, Newletter, 1993.10:120～123
30. Akagi H, Yolozeki A, et al. Highly polymorphic microsatellite of rice consist of AT repeats, and a classification of closely related cultivars with these microsatellite loci. Theor Appl Genet, 1997,94:61～67
31. Akkaya MS, Bhagwat AA, Cregan PB. Length polymorphisms of simple sequence repeat DNA in soybean[J]. Genetics, 1992, 132:1131～1139
32. Ayres NM, Mcclung AM, Larkin P D, et al. Microsatellites and a simple nucleotide polymorphism differentiate apparent amylose classes in extended pedigree of US rice germplasm[J]. Theor Appl Genet, 1997,94(5)773～781
33. Bohn M, M Khairallah D.QTL mapping in tropical maize: Ⅰ. Genomic region affecting leaf feeding resistance to sugarcane borer and other traits[J]. Crop Sci, 1996, 36(2): 1352～1361
34. Botstei D, White RL, Skolnick M, et al. Construction of a genetic map in man using restriction fragment polymorphisms[J]. Am J Hum Genet, 1980, 32: 314～331
35. Caetanao AG. DNA amplification fingerprinting using very short arbitrary oligonucleotide primers[J]. Biotechnology, 1991, 9:553～557
36. Causs MA . Saturated molecular map of the rice genome based on an interspecfic backcross population[J]. Genetics, 1994,138:1251-1274
37. Chaparro JX, Werner DJ, O'Malley D. et al. Targeted mapping and linkageanalysis of
morphological isozyme, and RAPD markers in peach [J]. Theor .Appl .Genet, 1994, 87: 805～815
38. Chert X , Temnynkh S , et al . Development of a microsatellite map providing genome-wide coverage in rice(Oryza sativa L .). Theor Appl Gnent, 1997. 95: 553～567
39. Cho YG, Eun MY, McCouch SR, Chac YA. The semidwarf gene, sd～1, of rice(Oryza sativa L ), Ⅱ, Molecular mapping and marker-assisted selection. Theor-Appl Genet, 1994, 89:54～59
40. Dib c, Faure S, et al. A comprehensive genetic map of the human genome based on 5264 microsatellite. Nature, 1996,380:149～152
41. Dietrich WF, Miller J. A comprehensive genetic map of the mouse genome[J]. Nature, 1996, 380:149～152
42. Edwards M, Johnson L. RFLP for rapid recurrent selection . Analysis of Molecular Marker Data, 1994, 33～40
43. Edwards MD, Page NJ. Evaluation of marker-assisted selection through computer simulation. Theor Appl Genet, 1994, 88:376～382
44. Ghaveyazie B. Classification of rice germ plasma Ⅰ: Analysis using AFLP and PCR-based RFLP[J]. Theor. Appl. Genet, 1995, 91 : 218～227
45. Gimelfarb A, Lande R. Marker-assisted selection and marker-QTL associations in hybrid populations. Theor Appl Genet, 1995, 91: 522～528
46. Gu WK, Weeden NF, Yu J, et al. Large-scale, cost-effective screening of PCR products in marker-assisted selection application[J]. Theor Appl Genet, 1995, 91 : 465～470
47. Haley SD, Miklas PN, Afanador L, and Kelly JD. Random amplified polymorphic DNA marker variability between and within gene pools of common bean. J.Amer.Soc.Hort Sci, 1994, 119:112～125
48. Han F, RomagosaI, Ullrich SE, Jones BL, Hayes PM, Wesenberg DM. Molecular marker-assisted selection for malting quality traits in barley. Mol Breed, 1997. 3: 427～437
49. Harushima Y, Fulton T M. Cho Y G ,et al. A high- density rice linkage map with 2275 markers using a single F_2 population[J]. Genetics ,1998 ,148:479～494
50. Helentjarls T. A genetic linkage map for maize based on RFLPs[J]. Trends in Gen, 1987, 3: 217～221
51. Hernandez P. Marlin A, and Dorado G. Development of SCARs by direct sequencing of RAPD products: a practical tool for the introgression and marker-assisted selection of wheat. Mol Breed. 1990. 5:245～253
52. Hittalmani S, Foolad MR, Mew T, et al. Development of a PCR-based marker to identify rice blast resistant gene Pi-Z(t) in a segregating population[J]. Theor Appl Genet, 1995, 91:9～14
53. Jouke PK, Herman JVE, Ronald GVB. The potential of AFLPs in biosystematics: a first application in Solanum taxonomy (Solanaceae)[J]. PI .Syst .Evol. 1998, 210:87～103
54. J. Zhang, Y. Xu et al. Bentazon and sulfonylurea sensitive mutant: breeding, genetics and potential application in seed production of hybrid rice. Theor Appl Genet. 2002. 105:16～12
55. Knapp SJ. Marker-assisted selection as a strategy for increasing the probability of selection superior genotypes. Crop Sci, 1998,38:1164～1174
56. Konieczny A, Ausubel FM. A procedure for mapping Arabidopsis mutations using co-dominant ecotype-specific PCR-based markers [J]. The Plant Journal, 1993, 4(2): 403～410
57. Lande R, and Thompson R. Efficiency of marker-assisted selection in the improvement of quantitative traits. Genetics, 1990, 124:743～756
58. Lander ES, Green P, Abrahamson J, et al. MAPMAKER: an interactive computer package for constructing primary genetic linkage map of experimental and natural populations. Genomics, 1987, 1: 174～181
59. Leister D, Ballvora A, Salamini F, et al. A PCR-based approach for isolation pathogen resistance genes from potato with a potential for wide application in plants [J]. Nature Genet, 1996, 14:421～429
60. Levi A. Thomas CE, Zhang X, et al. A genetic linkage map for watermelon based on randomly amplified polymorphic DNA markers [J]. Amer .Soc. Hort .Sci, 2001, 126 (6):730～737
61. Lin JJ. Identification of molecular markers in soybean: comparing RFLP, RAPD and AFLP DNA polymerase[J]. Plant Mol Biol Rep, 1996, 14:156～169
62. Lindhout P. Perspectives of molecular marker assisted breeding for earliness in tomato[J]. Euphytica, 1994, 79:279～286
63. Luc, Shen L, Tan Z, et al. Comparative mapping of QTLs for agronomic traits of rice cross environments by using a doubled -haploid population[J] . Theor Appl Genet. 1997, 94:145～150
64. M.A. Saghai, MQ. Zhang, DB. Neale, et al. Associations Between Nuclear Loci and Chloroplast DNA Genotypes in Wild Barley [J]. Genetics, 1992, 131 : 225～231
65. Mackill D J, Salam MA, Wang ZY. and Tanksley SD. A major photoperiod-sensitivity
gene tagged with RFLP and isozyme markers in rice. Theor Appl Genet, 1993, 85: 535～540
66. McCouch SR, Chen X , et al, Microsatellite marker development , mapping and application in rice genetics and breeding. Plant Mol Bio, 1997,35:89～99
67. Mccouch SR, Kochert G, Yu Z H, et al. Molecular mapping of rice chromosomes[J]. Theor Appl Genet ,1988,76:815～829
68. Michelmore RW, I Paran & RV Kesseli, Identification of markers linked to disease resistance genes by bulked segregant analysis: A rapid method to detect markers in specific genomic region by using segregating population. Proc Natl Acad Sci USA, 1991,88:9828～9832
69. Mohan M, Nair S, Bhagwat A, Krishna TG, Yano M, Bhatia Sasaki T. Genome mapping, Molecular markers and marker-assisted selection in crop plants. Molecular Breeding, 1997, 3:87～103
70. Moreau L, Charcosset A, Hospital F, Gallais A. Marker-assisted selection efficiency in populations of finite size. Genetics, 1998, 148:1353～1365
71. Mori K. Inheritance of susceptible mutant in rice plant to herbicide bentazon. Japan J Breeding, 1984,34(Suppl 1):421～422
72. Nakamcra YM, Leppert P, P O' Connell, et al. Variable number tandem repeat(VNTR)markers for human gene mapping[J]. Science, 1987, 235:1616～1622
73. Olson M, Hood L, Cantor C, et al. A common language for physical mapping of human genome [J]. Science, 1989, 254:1434～1435
74. Olufowote JO, Xu Y, et al. Comparative evaluation of within-cultivar variation of rice(Oryza sativa L .) using microsatellite and RFLP markers. Genome, 1997 , 40: 782～790
75. Panaud O, Chen X, et al. Frequency of microsatellite sequence in rice (Oryza sativa L .). Genome, 1995,38:1170～1176
76. Panaud O, Chen X, McCouch SR. Development of microsatellite markers and characteristics of simple sequence length polymorphism (SSLP) in rice(Oryza sativa L .) Mol Gen Genet,1996, 252:597～607
77. Paran I, Kesseli R, Michelmore R. Identification of restriction fragment length polymorphism and random amplified polymorphic DNA markers linked to a downy mildew resistance genes in lettuce using near-isogenic lines [J]. Genome, 1991, 34: 1021～1027
78. Sarkar GS, Kapelner S, Sommer SS. Formamide can dramatically improve the specificity of PCR[J]. Nucleic Acids Res, 1990, 18: 7465
79. Shi Liming. Genetic diversity and its preservation[J].Chinese Biodiversity, 1993(1) : 23～31
80. Voorrips RE, Jomgerius MC, Kanne HJ. Mapping of two genes for resistance to clubroot (Plasmodiophora brassicae) in a population of double haploid lines of Brassica oleracea by means of RFLP and AFLP markers[J].cc 1997,94(1):75～82
81. Vos P, Hogers R, Blecker M, et al. AFLP: a new technique for DNA fingerprinting[J]. Nucl. Acids. Res, 1995, 23: 4407～4414
82. Wang DG, Fan JB, Siao C J, et al. Large-scale identification, mapping, and genotyping of single-nucleotide polymorphisms in the human genome[J]. Science, 1998, 280: 1077～1082
83. Wang Z, Weber J L, et al. Survey of plant short tandem DNA repeats. Theor Appl Genet, 1994,88:1～6
84. Weber J, May PE. Abundant class of human DNA polymorphism which can be typed using the polymerase chain reaction [J]. Am .J .Genet, 1989,44:388～396
85. Welsh J, M. AcClelland. Fingerprinting genomes using PCR with arbitrary primers [J]. Nucl. Acids. Res, 1990, 18:7213～7218
86. Williams J, Kubelik A, Livak K, et al. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers [J]. Nucl. Acids. Res, 1990, 18: 6531～6535
87. Yanagihara S, McCouch SR, et al. Molecular analysis of the inheritance of the S-5 locus, conferring wide compatibility in Indica/Japonica hybrids of rice (Oryza sativa L .). Theor Appl Genet, 1995, 90:182～188
88. Zietkewicz E, Rafalski A, Labuda D. Genome fingerprinting by simple sequence repeat (SSR) -anchored polymerase chain reaction amplification[J]. Genomics, 1994, 20: 176～183
2．[3]董凤高，朱旭东，熊振民，等 以淡绿叶为标记的籼型光-敏核不育系的选育[J]中国水稻科学，1995，9(2)：65～70
3．邓华凤，李必湖，刘友芳，等．水稻光温敏核不育系起点温度提纯的实践与探讨[J]：杂交水稻，1997(6)：7～9．
4．黄大年，李敬阳等．用抗除草剂基因快速检测和提高杂交稻纯度的新技术．科学通报，1998，43(1)：67～70
5．李秀梅，吴竞仑等．国产苯达松在移栽稻田的除草效果和安全性．江苏农业科学，1996(4)：35～37
6．廖伏明，袁隆平．水稻光温敏核不育系起点温度遗传纯化的策略探讨[J]．杂交水稻，1996(6)：1～4．
7．刘树兵，王洪刚，孔令让等．高等植物的遗传作图[J]．山东农业大学学报(自然科学版)，1999，30(1)：73～78
8．陆作楣．我国杂交稻制种的基本成就与当前的问题和建议．杂交水稻，1989年增刊，1～4．
9．陆作楣等．杂交稻混杂退化问题研究．中国农业科学，1982(3)：8～15
10．陆作楣等．论我国杂交稻亲本繁育技术的演变．杂交水稻，1993(2)：1～3
11．陆作楣等．水稻雄性不育系自交结实问题之探讨．南京农业大学学报，1989，12(2)：4～11
12．罗孝和，邱趾忠，李任华．导致不育临界温度低的两用不育系培矮 64S[J]．杂交水稻，1992(2)：27～29．
13．马文宾，庄杰云，彭应财等．三系杂交稻亲本随几扩增多态性 DNA(RAPD)分析[J]．遗传，1998，20(2)：1～4
14．牟同敏，李春海，杨国才，等 紫叶水稻苗期叶色的遗传研究[J] 中国水稻科学，1995，9(1)：45～48
15．申宗坦主编．水稻籼粳亚种间杂种优势利用研究进展．浙江省881 2攻关计划论文集．北京：中国农业科技出版社．
16．沈又佳，陆作楣．种子纯度鉴定方法的研究——“赣化二号”杂交水稻种子的芽鞘色鉴定法．种子，1985(3)：15～186
17．苏少泉主编．除草剂作用靶标与新品种创制．北京：化学工业出版社，2001
18．谭震波．近等基因系和群分法在植物基因定位中的应用．遗传，1996，18(增刊)：27～30
19．唐梅，何光华，裴炎．中籼杂交水稻亲本多态性的AFLP分析[J]．遗传，2002，24(4)：439～441
20．王镭，郑茂波．遗传标记的研究进展[J]．生物技术，2002，12(2)：41～42
21．西北农学院．作物育种学[M]．北京：农业出版社，1991．42．
22．肖层林，周承恕．两系杂交稻种子纯度的影响因素与保纯技术．杂交水稻，2000，15(2)：12～14．
23．徐云碧．分子标记在数量基因定位中的应用[J]．遗传，1992，14(2)：45～48
24．袁隆平．水稻光温敏核不育系的提纯和原种生产[J]．杂交水稻，1994(6)：1．
25．张光恒，钱前等．试论杂交水稻种子纯度分析技术及其研究进展．种子，1909(1)：31～33
26．张亚兵，孔令传，刘华开，等．野败型杂交水稻种子纯度室内幼苗鉴定方法研究．杂交水稻，1997，12(3)：16～18
27．周天理等．水稻不育系中杂株的来源及遗传分析．作物学报，1992(5)：12～14．
28．朱启升，王安东等．水稻除草剂敏感基因导入恢复系的研究．杂交水稻，2000，15(3)：5～6
29. Abenes MLP. Selection of bacterial leaf blight resistance plants in the F_2 generation via their linkage to molecular markers[J]. Rice Genet, Newletter, 1993.10:120～123
30. Akagi H, Yolozeki A, et al. Highly polymorphic microsatellite of rice consist of AT repeats, and a classification of closely related cultivars with these microsatellite loci. Theor Appl Genet, 1997,94:61～67
31. Akkaya MS, Bhagwat AA, Cregan PB. Length polymorphisms of simple sequence repeat DNA in soybean[J]. Genetics, 1992, 132:1131～1139
32. Ayres NM, Mcclung AM, Larkin P D, et al. Microsatellites and a simple nucleotide polymorphism differentiate apparent amylose classes in extended pedigree of US rice germplasm[J]. Theor Appl Genet, 1997,94(5)773～781
33. Bohn M, M Khairallah D.QTL mapping in tropical maize: Ⅰ. Genomic region affecting leaf feeding resistance to sugarcane borer and other traits[J]. Crop Sci, 1996, 36(2): 1352～1361
34. Botstei D, White RL, Skolnick M, et al. Construction of a genetic map in man using restriction fragment polymorphisms[J]. Am J Hum Genet, 1980, 32: 314～331
35. Caetanao AG. DNA amplification fingerprinting using very short arbitrary oligonucleotide primers[J]. Biotechnology, 1991, 9:553～557
36. Causs MA . Saturated molecular map of the rice genome based on an interspecfic backcross population[J]. Genetics, 1994,138:1251-1274
37. Chaparro JX, Werner DJ, O'Malley D. et al. Targeted mapping and linkageanalysis of
morphological isozyme, and RAPD markers in peach [J]. Theor .Appl .Genet, 1994, 87: 805～815
38. Chert X , Temnynkh S , et al . Development of a microsatellite map providing genome-wide coverage in rice(Oryza sativa L .). Theor Appl Gnent, 1997. 95: 553～567
39. Cho YG, Eun MY, McCouch SR, Chac YA. The semidwarf gene, sd～1, of rice(Oryza sativa L ), Ⅱ, Molecular mapping and marker-assisted selection. Theor-Appl Genet, 1994, 89:54～59
40. Dib c, Faure S, et al. A comprehensive genetic map of the human genome based on 5264 microsatellite. Nature, 1996,380:149～152
41. Dietrich WF, Miller J. A comprehensive genetic map of the mouse genome[J]. Nature, 1996, 380:149～152
42. Edwards M, Johnson L. RFLP for rapid recurrent selection . Analysis of Molecular Marker Data, 1994, 33～40
43. Edwards MD, Page NJ. Evaluation of marker-assisted selection through computer simulation. Theor Appl Genet, 1994, 88:376～382
44. Ghaveyazie B. Classification of rice germ plasma Ⅰ: Analysis using AFLP and PCR-based RFLP[J]. Theor. Appl. Genet, 1995, 91 : 218～227
45. Gimelfarb A, Lande R. Marker-assisted selection and marker-QTL associations in hybrid populations. Theor Appl Genet, 1995, 91: 522～528
46. Gu WK, Weeden NF, Yu J, et al. Large-scale, cost-effective screening of PCR products in marker-assisted selection application[J]. Theor Appl Genet, 1995, 91 : 465～470
47. Haley SD, Miklas PN, Afanador L, and Kelly JD. Random amplified polymorphic DNA marker variability between and within gene pools of common bean. J.Amer.Soc.Hort Sci, 1994, 119:112～125
48. Han F, RomagosaI, Ullrich SE, Jones BL, Hayes PM, Wesenberg DM. Molecular marker-assisted selection for malting quality traits in barley. Mol Breed, 1997. 3: 427～437
49. Harushima Y, Fulton T M. Cho Y G ,et al. A high- density rice linkage map with 2275 markers using a single F_2 population[J]. Genetics ,1998 ,148:479～494
50. Helentjarls T. A genetic linkage map for maize based on RFLPs[J]. Trends in Gen, 1987, 3: 217～221
51. Hernandez P. Marlin A, and Dorado G. Development of SCARs by direct sequencing of RAPD products: a practical tool for the introgression and marker-assisted selection of wheat. Mol Breed. 1990. 5:245～253
52. Hittalmani S, Foolad MR, Mew T, et al. Development of a PCR-based marker to identify rice blast resistant gene Pi-Z(t) in a segregating population[J]. Theor Appl Genet, 1995, 91:9～14
53. Jouke PK, Herman JVE, Ronald GVB. The potential of AFLPs in biosystematics: a first application in Solanum taxonomy (Solanaceae)[J]. PI .Syst .Evol. 1998, 210:87～103
54. J. Zhang, Y. Xu et al. Bentazon and sulfonylurea sensitive mutant: breeding, genetics and potential application in seed production of hybrid rice. Theor Appl Genet. 2002. 105:16～12
55. Knapp SJ. Marker-assisted selection as a strategy for increasing the probability of selection superior genotypes. Crop Sci, 1998,38:1164～1174
56. Konieczny A, Ausubel FM. A procedure for mapping Arabidopsis mutations using co-dominant ecotype-specific PCR-based markers [J]. The Plant Journal, 1993, 4(2): 403～410
57. Lande R, and Thompson R. Efficiency of marker-assisted selection in the improvement of quantitative traits. Genetics, 1990, 124:743～756
58. Lander ES, Green P, Abrahamson J, et al. MAPMAKER: an interactive computer package for constructing primary genetic linkage map of experimental and natural populations. Genomics, 1987, 1: 174～181
59. Leister D, Ballvora A, Salamini F, et al. A PCR-based approach for isolation pathogen resistance genes from potato with a potential for wide application in plants [J]. Nature Genet, 1996, 14:421～429
60. Levi A. Thomas CE, Zhang X, et al. A genetic linkage map for watermelon based on randomly amplified polymorphic DNA markers [J]. Amer .Soc. Hort .Sci, 2001, 126 (6):730～737
61. Lin JJ. Identification of molecular markers in soybean: comparing RFLP, RAPD and AFLP DNA polymerase[J]. Plant Mol Biol Rep, 1996, 14:156～169
62. Lindhout P. Perspectives of molecular marker assisted breeding for earliness in tomato[J]. Euphytica, 1994, 79:279～286
63. Luc, Shen L, Tan Z, et al. Comparative mapping of QTLs for agronomic traits of rice cross environments by using a doubled -haploid population[J] . Theor Appl Genet. 1997, 94:145～150
64. M.A. Saghai, MQ. Zhang, DB. Neale, et al. Associations Between Nuclear Loci and Chloroplast DNA Genotypes in Wild Barley [J]. Genetics, 1992, 131 : 225～231
65. Mackill D J, Salam MA, Wang ZY. and Tanksley SD. A major photoperiod-sensitivity
gene tagged with RFLP and isozyme markers in rice. Theor Appl Genet, 1993, 85: 535～540
66. McCouch SR, Chen X , et al, Microsatellite marker development , mapping and application in rice genetics and breeding. Plant Mol Bio, 1997,35:89～99
67. Mccouch SR, Kochert G, Yu Z H, et al. Molecular mapping of rice chromosomes[J]. Theor Appl Genet ,1988,76:815～829
68. Michelmore RW, I Paran & RV Kesseli, Identification of markers linked to disease resistance genes by bulked segregant analysis: A rapid method to detect markers in specific genomic region by using segregating population. Proc Natl Acad Sci USA, 1991,88:9828～9832
69. Mohan M, Nair S, Bhagwat A, Krishna TG, Yano M, Bhatia Sasaki T. Genome mapping, Molecular markers and marker-assisted selection in crop plants. Molecular Breeding, 1997, 3:87～103
70. Moreau L, Charcosset A, Hospital F, Gallais A. Marker-assisted selection efficiency in populations of finite size. Genetics, 1998, 148:1353～1365
71. Mori K. Inheritance of susceptible mutant in rice plant to herbicide bentazon. Japan J Breeding, 1984,34(Suppl 1):421～422
72. Nakamcra YM, Leppert P, P O' Connell, et al. Variable number tandem repeat(VNTR)markers for human gene mapping[J]. Science, 1987, 235:1616～1622
73. Olson M, Hood L, Cantor C, et al. A common language for physical mapping of human genome [J]. Science, 1989, 254:1434～1435
74. Olufowote JO, Xu Y, et al. Comparative evaluation of within-cultivar variation of rice(Oryza sativa L .) using microsatellite and RFLP markers. Genome, 1997 , 40: 782～790
75. Panaud O, Chen X, et al. Frequency of microsatellite sequence in rice (Oryza sativa L .). Genome, 1995,38:1170～1176
76. Panaud O, Chen X, McCouch SR. Development of microsatellite markers and characteristics of simple sequence length polymorphism (SSLP) in rice(Oryza sativa L .) Mol Gen Genet,1996, 252:597～607
77. Paran I, Kesseli R, Michelmore R. Identification of restriction fragment length polymorphism and random amplified polymorphic DNA markers linked to a downy mildew resistance genes in lettuce using near-isogenic lines [J]. Genome, 1991, 34: 1021～1027
78. Sarkar GS, Kapelner S, Sommer SS. Formamide can dramatically improve the specificity of PCR[J]. Nucleic Acids Res, 1990, 18: 7465
79. Shi Liming. Genetic diversity and its preservation[J].Chinese Biodiversity, 1993(1) : 23～31
80. Voorrips RE, Jomgerius MC, Kanne HJ. Mapping of two genes for resistance to clubroot (Plasmodiophora brassicae) in a population of double haploid lines of Brassica oleracea by means of RFLP and AFLP markers[J].cc 1997,94(1):75～82
81. Vos P, Hogers R, Blecker M, et al. AFLP: a new technique for DNA fingerprinting[J]. Nucl. Acids. Res, 1995, 23: 4407～4414
82. Wang DG, Fan JB, Siao C J, et al. Large-scale identification, mapping, and genotyping of single-nucleotide polymorphisms in the human genome[J]. Science, 1998, 280: 1077～1082
83. Wang Z, Weber J L, et al. Survey of plant short tandem DNA repeats. Theor Appl Genet, 1994,88:1～6
84. Weber J, May PE. Abundant class of human DNA polymorphism which can be typed using the polymerase chain reaction [J]. Am .J .Genet, 1989,44:388～396
85. Welsh J, M. AcClelland. Fingerprinting genomes using PCR with arbitrary primers [J]. Nucl. Acids. Res, 1990, 18:7213～7218
86. Williams J, Kubelik A, Livak K, et al. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers [J]. Nucl. Acids. Res, 1990, 18: 6531～6535
87. Yanagihara S, McCouch SR, et al. Molecular analysis of the inheritance of the S-5 locus, conferring wide compatibility in Indica/Japonica hybrids of rice (Oryza sativa L .). Theor Appl Genet, 1995, 90:182～188
88. Zietkewicz E, Rafalski A, Labuda D. Genome fingerprinting by simple sequence repeat (SSR) -anchored polymerase chain reaction amplification[J]. Genomics, 1994, 20: 176～183
© 2004-2018 中国地质图书馆版权所有 京ICP备05064691号 京公网安备11010802017129号 地址：北京市海淀区学院路29号 邮编：100083 电话：办公室：(+86 10)66554848；文献借阅、咨询服务、科技查新：66554700 |