中医阳黄证黄疸动物模型的建立及中药退黄对模型影响的实验研究
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摘要
目的：建立中医阳黄证黄疸动物模型。方法：在采用现代医学异硫氢酸苯酯(APIT)灌胃诱导大鼠肝损伤的基础上，结合中医高脂高糖饮食加湿热环境因素，建立中医阳黄证黄疸动物模型。观察大鼠一般生物学状态、肝功能变化及体液免疫水平，并予中药复健方茵陈蒿汤治疗。从病因、症状、体征、实验指标以及中药治疗反证等方面对模型加以评价。结果：中医阳黄证黄疸动物模型符合黄疸证疾病特点，未改变黄疸模型的关键指标，未影响异硫氢酸苯酯(APIT)灌胃造模方法的主要特点；同时模型大鼠出现明显湿热证主证及湿热证客观指标变化，经中药复健方治疗后，黄疸证指标和湿热证主证及指标明显改善。结论：采用现代医学异硫氢酸苯酯(APIT)灌胃，结合中医高脂高糖饮食加湿热环境因素建立的中医阳黄证黄疸动物模型符合中医学传统病因病机理论，方法可靠，重现性强，与人类阳黄证相似，为中医药研究治疗黄疸阳黄证提供了较为合适的动物模型。
Purpose: To establish an animal model of TCM jaundice of Yang-Huang. Materials and Methods: We got the animal .model of TCM jaundice of Yang-Huang in rats, which lived in the damp and hot environment and feed on high-fat-sucrose diet , with the injury of liver induced by oral APIT. We observe the general biological state, liver function, humoral immunity, and the changes after treating with Chinese herbal decoction .("Yinchenhao " decoction) in rat. The model was evaluated with etiologic factor, sumptom, sign, experimental data, and the disproof of treating with herbal decoction.
Result: The model of TCM jaundice of Yang-Huang in rat fit the character of jaundice, which didn't change key point marke of jaundice model, didn't effect 昪hief behavior of APIT jaundice model. The rats of the model all have the symptoms of dampness-heat syndrome and have the significant changes in experimental data of dampness-heat syndrome. The experimental data of jaundice and dampness-heat syndrome are both improved significantly treating with herbal decoction.
Conclusion: The animal model of TCM jaundice of Yang-Huang in rats, which lived in the damp and hot environment and feed on ? , with the injury of liver induced by oral APIT is in accord with the TCM theory in etiology and pathology and similar with the clinical symptom in human. It is suitable and potentially useful in the study of TCM treatment of jaundice.
Purpose: To establish an animal model of TCM jaundice of Yang-Huang. Materials and Methods: We got the animal .model of TCM jaundice of Yang-Huang in rats, which lived in the damp and hot environment and feed on high-fat-sucrose diet , with the injury of liver induced by oral APIT. We observe the general biological state, liver function, humoral immunity, and the changes after treating with Chinese herbal decoction .("Yinchenhao " decoction) in rat. The model was evaluated with etiologic factor, sumptom, sign, experimental data, and the disproof of treating with herbal decoction.
Result: The model of TCM jaundice of Yang-Huang in rat fit the character of jaundice, which didn't change key point marke of jaundice model, didn't effect 昪hief behavior of APIT jaundice model. The rats of the model all have the symptoms of dampness-heat syndrome and have the significant changes in experimental data of dampness-heat syndrome. The experimental data of jaundice and dampness-heat syndrome are both improved significantly treating with herbal decoction.
Conclusion: The animal model of TCM jaundice of Yang-Huang in rats, which lived in the damp and hot environment and feed on ? , with the injury of liver induced by oral APIT is in accord with the TCM theory in etiology and pathology and similar with the clinical symptom in human. It is suitable and potentially useful in the study of TCM treatment of jaundice.
引文
[1] 张赤志．阴黄证动物模型的建立及其意义．中国中医基础医学杂志，2000；6(7)：43
[2] 卢家兵．阴黄证外周血淋巴细胞凋亡及基因调控的研究．中西医结合肝病杂志，2002；12(3)：150-152
[3] 杨春波，等．慢性胃炎脾胃湿热证的免疫组织化学研究．中国中西医结合脾胃杂志，1998：6(2)：69
[4] 余江毅．慢性肾病瘀血与湿热病理的临床和实验研究．辽宁中医杂志，1995；22(2)：91
[5] 王瑾．中医温病湿热证动物模型实验的研究．解放军药学学报，2002：18(4)：209
[6] 陈爽白．湿热证大鼠模型的复制及三仁汤对其影响的实验观察．天津中医，2002：19(2)：37
[7] 陈压西．小柴胡汤胶囊抗鸭乙型肝炎病毒的实验研究．中西医结合肝病杂志，2000；10(2)：17
[8] 许建明．短小棒状杆菌和脂多糖诱发的免疫性肝损伤模型．中国药理学通报，1997：13(2)：186
[9] 夏秦．一氧化氮对小鼠急性重型肝炎病理改变的影响．中西医结合肝病杂志，2000；10(5)：20
[10] 张亚兵．蛇葡萄根对刀豆蛋白(ConA)诱导小鼠肝损伤的防护作用．中西医结合肝病杂志，2000；10(1)：26
[11] 王永新．退黄合剂对药物诱发急性肝损伤小鼠保肝退黄作用的研究．中国实验方剂学杂志，2001：4：38
[12] 王立秋．TNT致肝损伤大鼠的血清胆红素HPLC测定．中华劳动卫生职业病杂志，1995；2：65
[13] 陈奇．中药药理研究方法学．北京：人民卫生出版社，1993；9．724
[14] 李东良．大鼠酒精性肝损伤模型建立及病理学观察．中西医结合肝病杂志，2000，10(2)：30
[15] 李桦．精氨酸对阻塞性黄疸大鼠免疫功能的影响．中华实验外科杂志．1991，(3)：135
[2] 卢家兵．阴黄证外周血淋巴细胞凋亡及基因调控的研究．中西医结合肝病杂志，2002；12(3)：150-152
[3] 杨春波，等．慢性胃炎脾胃湿热证的免疫组织化学研究．中国中西医结合脾胃杂志，1998：6(2)：69
[4] 余江毅．慢性肾病瘀血与湿热病理的临床和实验研究．辽宁中医杂志，1995；22(2)：91
[5] 王瑾．中医温病湿热证动物模型实验的研究．解放军药学学报，2002：18(4)：209
[6] 陈爽白．湿热证大鼠模型的复制及三仁汤对其影响的实验观察．天津中医，2002：19(2)：37
[7] 陈压西．小柴胡汤胶囊抗鸭乙型肝炎病毒的实验研究．中西医结合肝病杂志，2000；10(2)：17
[8] 许建明．短小棒状杆菌和脂多糖诱发的免疫性肝损伤模型．中国药理学通报，1997：13(2)：186
[9] 夏秦．一氧化氮对小鼠急性重型肝炎病理改变的影响．中西医结合肝病杂志，2000；10(5)：20
[10] 张亚兵．蛇葡萄根对刀豆蛋白(ConA)诱导小鼠肝损伤的防护作用．中西医结合肝病杂志，2000；10(1)：26
[11] 王永新．退黄合剂对药物诱发急性肝损伤小鼠保肝退黄作用的研究．中国实验方剂学杂志，2001：4：38
[12] 王立秋．TNT致肝损伤大鼠的血清胆红素HPLC测定．中华劳动卫生职业病杂志，1995；2：65
[13] 陈奇．中药药理研究方法学．北京：人民卫生出版社，1993；9．724
[14] 李东良．大鼠酒精性肝损伤模型建立及病理学观察．中西医结合肝病杂志，2000，10(2)：30
[15] 李桦．精氨酸对阻塞性黄疸大鼠免疫功能的影响．中华实验外科杂志．1991，(3)：135
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