伪狂犬病病毒gD基因的生物信息学分析
|
摘要
从2004．6-2005．6年四川省内各规模化大型猪场出现的流产死胎14头中，通过PCR检测出2头为阳性，并从中成功的分离到两株伪狂犬病病毒株，将其分别命名为SS株和SQ株。将所有的毒株连同四川农业大学动物生物技术中心保存Fa株、疫苗株783株、Bartha株和SA215株，四川野毒株SN株、SL株和SCZ株，以及由韶关学院英东生物工程学院娄高明教授惠赠的北京株BJ株、广东株DG、湖北株HS株共计12株伪狂犬病病毒株，应用PCR技术扩增得到了PRV完整的gD基因的片段12条。回收PCR产物，成功连接转化到感受态细胞E．coli DH_(5a)中，并通过菌落PCR和酶切鉴定正确后测序。
将所的12条序列连同GenBank中登录的9条gD基因全序列共21条基因序列，使用生物软件对它们的基因序列的同源性、突变区域的定位、遗传进化关系、酶切位点的差异、密码子偏爱性，氨基酸序列的同源性、蛋白质亲水性、抗原表位分析、二级结构、三级结构预测等生物信息学的内容进行预测和分析。结果表明：PRV-gD基因的开放阅读框的核苷酸长度在1197～1215nt之间，氨基酸长度在399～405个之间，核酸同源性在97.3％-100％之间，氨基酸的同源性在89.8％-98.8％之间，在核酸820-837位有个高变重复区，不同毒株基因均对GC极为偏爱。在遗传进化关系上将我国PRV流行分为四川、华北、东南三个区域。毒株间基因内酶切位点、蛋白质亲水性、抗原表位和蛋白质高级结构预测等内容的分析结果十分相似，说明PRV-gD基因具有很高的保守性。该研究从病原、分子水平、生物信息等方面对PRV-gD基因进行了研究，对于PRV的流行病学研究及诊断和防治具有重要意义。
Two pig's abortus was resulted of pseudorabies virus by gD-PCR detected in 14 pig's abortus which were picked up from several large-scale hogpen in Sichuan province between 2004. 6-2005. 6. And then we Isolated two pseudorabies virus strain from these two pig's abortus successfully.They are named SS and SQ according to where they were picked up.There are 12 pseudorabies virus strain ,SS、SQ and Fa (saved by Sichuan Agriculture University Animal biotechnology center) and SA215 、783strain 、 Bartha(purchased from China Animal Husbandry Industry CO.LTD)and SN、 SL、 SCZ(Isolated by Xu Zhiwen associate professor in Sichuan Agriculture University Animal biotechnology center inl998) and BJ、DG、HS (gived by Lou Gaoming professor in Shaoguan University College of Yingdong Biorngineering) . Designing a pair of primer according to PRV gD gene, we received 12 gD complete gene by PCR.We used TaKaRa' s kit to retrieve gel and to ligate, transform into E. coli DH5a, and picking singal white masculine masculine by Amp-X-gal-IPTG LB flat plate. After identified by COL-PCR and RE, they were sequenced .
We sequenced 12 PRV-gD gene and downloaded 9 gD's sequence , and then predict and analyzed their's Homology of nucleotide and amino acids sequence 、lacated mutation area、phylogentic tree、Enzymatic digestion site、 code preference、Hydrophobicity、 Predicting Antigenic helices and Three-dimensional structure by biology software.The result is : PRV-gD's ORF's length is between 1197-1215nt, amino acids' length is between 399～405; Homology of nucleotide sequence is between 97.3%~100%, Homology of amino acids sequence is between 89.8%~98.8%;PRV's code pref GC very much; phylogentic tree analusis result is make three district where PRV spreaded in Chian: Sichuan Province 、Northern Chinaan and Southeast China; and other result is very similar. All above is demonstrate; document;evid.;evidence;justify;proof;testify; verification;witness marks PRV-gD is very important to PRV and it has a heightly conservatism..
将所的12条序列连同GenBank中登录的9条gD基因全序列共21条基因序列，使用生物软件对它们的基因序列的同源性、突变区域的定位、遗传进化关系、酶切位点的差异、密码子偏爱性，氨基酸序列的同源性、蛋白质亲水性、抗原表位分析、二级结构、三级结构预测等生物信息学的内容进行预测和分析。结果表明：PRV-gD基因的开放阅读框的核苷酸长度在1197～1215nt之间，氨基酸长度在399～405个之间，核酸同源性在97.3％-100％之间，氨基酸的同源性在89.8％-98.8％之间，在核酸820-837位有个高变重复区，不同毒株基因均对GC极为偏爱。在遗传进化关系上将我国PRV流行分为四川、华北、东南三个区域。毒株间基因内酶切位点、蛋白质亲水性、抗原表位和蛋白质高级结构预测等内容的分析结果十分相似，说明PRV-gD基因具有很高的保守性。该研究从病原、分子水平、生物信息等方面对PRV-gD基因进行了研究，对于PRV的流行病学研究及诊断和防治具有重要意义。
Two pig's abortus was resulted of pseudorabies virus by gD-PCR detected in 14 pig's abortus which were picked up from several large-scale hogpen in Sichuan province between 2004. 6-2005. 6. And then we Isolated two pseudorabies virus strain from these two pig's abortus successfully.They are named SS and SQ according to where they were picked up.There are 12 pseudorabies virus strain ,SS、SQ and Fa (saved by Sichuan Agriculture University Animal biotechnology center) and SA215 、783strain 、 Bartha(purchased from China Animal Husbandry Industry CO.LTD)and SN、 SL、 SCZ(Isolated by Xu Zhiwen associate professor in Sichuan Agriculture University Animal biotechnology center inl998) and BJ、DG、HS (gived by Lou Gaoming professor in Shaoguan University College of Yingdong Biorngineering) . Designing a pair of primer according to PRV gD gene, we received 12 gD complete gene by PCR.We used TaKaRa' s kit to retrieve gel and to ligate, transform into E. coli DH5a, and picking singal white masculine masculine by Amp-X-gal-IPTG LB flat plate. After identified by COL-PCR and RE, they were sequenced .
We sequenced 12 PRV-gD gene and downloaded 9 gD's sequence , and then predict and analyzed their's Homology of nucleotide and amino acids sequence 、lacated mutation area、phylogentic tree、Enzymatic digestion site、 code preference、Hydrophobicity、 Predicting Antigenic helices and Three-dimensional structure by biology software.The result is : PRV-gD's ORF's length is between 1197-1215nt, amino acids' length is between 399～405; Homology of nucleotide sequence is between 97.3%~100%, Homology of amino acids sequence is between 89.8%~98.8%;PRV's code pref GC very much; phylogentic tree analusis result is make three district where PRV spreaded in Chian: Sichuan Province 、Northern Chinaan and Southeast China; and other result is very similar. All above is demonstrate; document;evid.;evidence;justify;proof;testify; verification;witness marks PRV-gD is very important to PRV and it has a heightly conservatism..
引文
[1] 殷震，刘景华．动物病毒学[M]．北京：科技出版社，1997．329～340；998～1002．
[2] Wittmann G. and Rziha, H.J. Herpesvrius diseases of cattle, horse, and pigs. In Developments in Veterinary Virology. Boston: Kluwer Academic Publishers, pp. 1989, 230-325
[3] 程道祥．伪狂犬病的感染传播与流行．[J]福建畜牧兽医．1999，21(1)：34-35
[4] 郭万柱、刘亚刚、娄高明．兽医病毒学[M]．成都：四川科学技术出版社，2003．140-142
[5] 吴清民．兽医传染病[M]．北京：中国农业大学出版社，2002．124-130
[6] 李三星．关于伪狂犬病．[J]河北农业大学学报，1989．12(1)：145-150．
[7] Beran G W, Davies E B, Arambulo P V, et aLPersistence of pseudorabies v″vus in infected swine. [J]J Am Vet Med Assoc, 1979, 176: 998-1001.
[8] Sabo A, Itajcani L. Latent pseudorabies infection in pigs. [J]Acta Virol, 1976, 20: 208-214.
[9] Rziha H-J, Mettenleiter C T, Ohlinger V, et al. Herpesvirus(pseudorabies vinrs)latency in swine: occurrence and physical state of viral DNA in neural tissues. [J]Virol, 1986, 155: 600-613.
[10] Belak S, Ballagi-Pordancy A, Fhsburg J, et al. Detection of pseudorabies virus DNA sequences by the polymaase chain reaction. [J]Arch Virol. 1989, 108: 279-286
[11] 蔡宝祥．家畜传染病学[M]．北京：中国农业出版社，1996，207-209
[12] 郭万柱、颜其贵．伪狂犬病预防控制研究进展．畜禽重大疫病生物技术防制研究[A]．北京：中国农业大学出版社，1998．156-166
[13] 陈斌。伪狂犬病病毒Fa株胸苷激酶基因克隆及PCR扩增诊断伪狂犬病病毒的研究[D]．雅安，四川农业大学，1996
[14] 童光玉等．猪伪狂犬病的诊断和防治，[J]中国兽医杂志，1993，19(8)：18-19
[15] 李树春等．仔猪伪狂犬病的诊断．[J]中国兽医杂志，1995，8，21
[16] 韦华姜等．湖北首次暴发猪伪狂犬病的报告．[J]中国畜禽传染病，1994，4，36-38
[17] 朱庆虎等．近10年我国伪狂犬病研究进展．[J]中国畜禽传染病，1996，6，59-61
[18]．刘正飞．伪狂犬病TK-／gE基因缺失疫苗研究．[D]．武汉：华中农业大学，2002
[19] 李成，刘树森，王继科，姜绍德，谷守林，郝桂玉，张坦．畜禽病毒图谱[M]．北京农业出版社，1987．15-16
[20] 郭万柱、吴彤、陈瑶先．动物微生物学[M]．成都：四川科学技术出版社，2001．327-328
[21] 许雁峰，郭万柱，石谦等．猪伪狂犬病病毒gD基因的研究进展[J]四川畜牧兽医2005年7期，19-20
[22] McGeoch, D. J., M. A. Dalrymple, A. J. Davison, et al. The complete DNA sequence of the long unique region in the genome of herpes simplex virus type 1[J]. J. Gen. Virol. 1988. 69(7): 1531-1574
[23] Dolan, A., F. E. Jamieson, C. Cunningham, et al. The genome sequence of herpes simplex virus type 2[J]. J. Virol. 1998.72(3): 2010-2021.
[24] Davison, A. J., and J. E. Scott. The complete DNA sequence of varicella-zoster virus[J]. J. Gen. Virol. 1986, 67(9): 1759-1816.
[25] Telford, E. A., M. S. Watson, K. McBride, et al. The DNA sequence of equine herpesvirus -1[J]. Virology 1992, 189(1): 304-316.
[26] Telford, E. A., M. S. Watson, J. Perry, et al. The DNA sequence of equine herpesvirus -4[J]. J. Gen. Virol. 1998, 79(5): 1197-1203.
[27] Tulman, E. R., C. L. Afonso, Z. Lu, et al. The genome of a very virulent Marek's disease virus[J]. J. Virol. 2000, 74(17): 7980-7988.
[28] Afonso, C. L., E. R. Tulman, Z. Lu, et al. The genome of turkey herpesvirus[J]. J. Virol. 2001, 75(2): 971-978.
[29] Barbara G. Klupp, Christoph J. Hengartner, Complete, Annotated Sequence of the Pseudorabies Virus Genome[J]. Journal of Virology, 2004(1): 424-440.
[30] 陈世界、郭万柱．伪狂犬病病毒分子生物学．畜禽重大疫病生物技术防制研究[A]．北京：中国农业大学出版社，1998．167-172
[31] Glenn F. Rall, Sabina Kupershmidt et al, Functions of the sequences at the ends of the inverted repeats of pseudorabies virus[J], Journal of Virology, Mar 1992: 1506-1519
[32] 余树民、郭万柱．伪狂犬病基因工程疫苗的研究现状．畜禽重大疫病生物技术防制研究[A]．北京：中国农业大学出版社，1998．179-184
[33] 王勤．伪狂犬病病毒Fa株gE基因的克隆与序列的比较分析[D]．雅安，四川农业大学，2000
[34] Menttenleiter. T.C 1991. Molecular biology of pseudorabies (Aujeszky's disease) virus[J]．Comp Immunol Microbiol Infect Dis 14：11-163
[35] 马相如．伪狂犬病病毒宿主关闭蛋白的基因克隆、表达及其结构分析[D]．武汉，华中农业大学，2005
[36] Mayra E M Campbell et al, DNA sequences which regulate the expression of pseudorabies virus major immediate early gene[J]. Virolgy(Vol157)1994, 307-326
[37] K L Glazenburg, B. P. H. Peeters et al, Construction and properties of pseudorabies virus recombinants with altered control of immediate early gene expression[J], Journal of virology, jane 1995, 189-197
[38] Suzette A, Priola Jack G Stevers. The 5' and 3' limits of transcription in the pseudorabies Virus latency associated transcription unit[J]. Virology, 1991, 182: 852-856
[39] Andrew K Cheung et al. Pseudorabies virus deletion mutants involving the EPO and LLT genes[J], U S Patent, Oct 1994, No. 5352596
[40] G ibsonW. St ructurea nda ssemblyo fth ev irion[J]. In tervirology, 1996, 39: 389-400
[41] F uchsW, K luppB G, G ranzowH, H engartnerC, Brackh, M undtA, E nquistL W, M etenleiterT C. Ph y sic alin teraction between envelop glycoproteinE and M of ps eudorabiesv irusand the major tegument protein UL49[J]. J Virol, 2002c, 76(16): 8208-8217
[42] G ershonh A, S hermanD L, Z huZ, G abelC A, A mbronR T, G ershonM D. Intracellular transport of newly synthesized varieellazoster virus: final envelopment in the trans-Golgi network[J]. J Virol, 2000, 68: 6372-6390
[43] Newcomb W N, Homa F L, Thomsen D R. Assembly of the herpes simplex virus procapsid from purified components and identification of small complexes containing the major capsid and scaffolding proteins[J]. J Virol, 1999, 73: 4239-425
[44] ZhouZ, Chen D, Jakana J. Visualization of tegument-capsid interactions and DNA in intact herpes simplex virus type Ⅰ virions[J]. J Virol, 1999, 73: 3210-3218
[45] 娄高明，杜伟贤．郭万柱伪狂犬病病毒糖蛋白研究进展[J]．广东畜牧兽医科技1999，24(4)：3-6
[46] 陈斌．伪狂犬病病毒及其免役研究进展(上)[J]．四川畜牧兽医2001，28(9)：32-34
[47] Takad A Kida H. Induction of protective antibody responwew against pseudorabies virus buintranasal vaccination with glycoproteinB inmice[J]. Arch-virol, 1995, 140(9): 1629-1635
[48] 张志，范伟兴．赵宏坤伪狂犬病病毒囊膜糖蛋白的研究进展[J]．山东农业大学学报(自然科学版)，2001，32(1)：85-89
[49] 范伟兴、赵宏坤．伪狂犬病病毒分子生物学研究新近展．中国畜牧兽医学会家畜传染病学分会第九次学术讨论会[A]．2001
[50] 王勤．猪伪狂犬病病毒gE基因的研究进展[J]．畜牧与兽医．2002，34(10)：42-44
[51] 宋耀彬．猪的伪狂犬病[J]．辽宁畜牧兽医．1982，1：35-38
[52] 阿都阿且等．家畜伪狂犬病的调查[J]．中国畜禽传染病．1992，63(2)，29-30
[53] 黄寿森等．牛伪狂犬病的研究(第一报)：牛伪狂犬病病毒的分离及若干生物学特性的研究[J]．畜牧兽医学报．1966，9(1)，22-29
[54] 倪杉．陕A株伪狂犬病病毒的分离与鉴定[J]．兽医科技资料．1979，2，12-18
[55] 佘柏荣等．广东吴川株伪狂犬病毒的鉴定[J]．中国兽送杂志．1986，12(7)，17-19
[56] 马崇文等．家畜伪狂犬病流行病学调查[J]．家畜传染病．1986，1，40-41
[57] 王永贤等．伪狂犬病毒YN株的分离与鉴定[J]．中国人民解放军兽医大学学报．1984，4(4)，317-321
[58] 韦贤金．耕牛伪狂犬病的诊断与防制[J]．广西兽医科技．1985，2，48-49
[59] 毕远龙．云南耕牛伪狂犬病病毒的理化特性与电镜观察[J]．家畜传染病．1986，4，44-46
[60] 李秉机．奶牛、绵羊伪狂犬病的初步观察[J]．中国兽医杂志．1983，6，21
[61] 叶公权．耕牛伪狂犬病病例的报告[J]．畜牧兽医科技(广东)．1987，3，29-32
[62] 肖裕波．疑似绵羊的伪狂犬病[J]．中国兽医杂志．1965，3(5)
[63] 王永洪等．一次猪、狗、猫伪狂犬病爆发流行的研究[J]，家畜传染病，19842，30-32
[64] 李成卓．马、鹿伪狂犬病的一次爆发[J]．中国兽医杂志．1983，4，22-23
[65] 佘永建．猪伪狂犬病[J]．畜牧与兽医．1987(1)：29-33
[66] 徐志文．四川省伪狂犬病分子流行病学研究[D]．雅安，四川农业大学，1998
[67] 何启盖、陈焕春．猪伪狂犬病新的流行病学特征[J]．华中农业大学学报2000增刊．总第33期
[68] 何启盖、刘正飞．猪伪狂犬病的防制研究进展[J]．河南畜牧兽医．2000，21(6)：9-11
[69] 唐万勇．猪伪狂犬病的病理学研究[D]．武汉：华中农业大学，2003
[70] Narita M, Inui S, Schimizu YTonsillar changes in pigs given pseudorabies (Aujeszky's disease) virus[J]. Am J Vet Res. 1984, 45(2): 247-251
[71] Kluge J P and Mare C J. Gross and microscopic lesions of prenatal and neonatal pseudorabies(Aujeszky's disease)virus in swine[J]. Proc Int Congro Pig Vet Soc 1976，4：63．
[72] 张斌、汪开毓、冯泽光、郭万柱．仔猪伪狂犬病病理学研究[J]．畜牧兽医学报．1992，23(2)：182-186
[73] Narita M ,Kubo M, FukushoA, Haritani M, Moriwaki M. Necrotizing enteritis in piglets associated with the virus of Aujeszky's disease[J].Veterinary Pathol. 1984.21:450-452.
[74] 蒋鹤春．伪狂犬病病毒gE基因的表达及初步应用[D]．南京：南京农业大学，2000
[75] 刘镇明、蓝天．猪伪狂犬病病毒粤株的分离与鉴定[J]．华南农业大学学报．2000，(21)2：76-78．
[76] 祁贤、张婉华．猪伪狂犬病病毒S1株的分离与鉴定．Cninese Journal of Veterinary Science and Technology [J].2002,132(4):24-26
[77] Gutekunst DE, Pirtle EC, Miller LD, Stewart WC.. Isolation of pseudorabies virus from trigeminal ganglia of a latently infected sow[J].Am J Vet Res. 1980 Aug;41(8):1315-1316
[78] Dangler CA, Henderson LM, Bowman LA, Dearer RE. Direct isolation and identification of recombinant pseudorabies virus strains from tissues of experimentally co-infected swine. Am J Vet Res. 1993 Apr;54(4):540-5.
[79] 吴雅玲．猪伪狂犬病[J]．青海畜牧兽医杂志．2001，31(2)：34-35
[80] 程由铨、吴平、林天龙等．4种检测伪狂犬病病毒抗原方法的的比较[J]．中国兽医杂志．1992，18(1)：6-9．
[81] 娄高明，陈志荣，郭万柱等．双抗体夹心ELISA检测伪狂犬病病毒的研究[J]．中国畜禽传染病，1998，20(4)：236～240
[82] 郭万柱、冯炳芳、曹军等．应用~(32)P标记DNA探针检测伪狂犬病病病毒的研究[J]．四川农业大学报．1991，9(1)：52 56
[83] 王琴、郭万柱．应用生物素标记DNA探针检测伪狂犬病病毒的研究[J]．中国病毒学．1996，(11)3：284-285
[84] 李学伍、陈焕春、周复春等．用地高辛标记的DNA探针检测伪狂犬病毒[J]．畜牧与兽医．1997，29(6)：270-271
[85] Maes R K, Sussman M D, Recent. Developments in Iatency and recombination of Aujeszky's disease (pseudorabies) virus[J]. Vet Microbiol. 1997,55:13-27.
[86] J. Galeota Wheeler, BA Investigation of sites of Pseudorabies virus latency using polymerase chain reaction[J]Am vet Res, 1991,52(11):1799-1803
[87] 周复春、陈焕春．伪狂犬病病毒PCR检测方法的建立[J]．华中农业大学学报．2000年增刊总第33期
[88] 冉多良、王正党、魏伟应．用聚合酶联反应检测伪狂犬病毒DNA[J]．中国兽医学报1994，14(4)：366-368
[89] 娄高明，杜伟贤，廖筱萍．PCR检测伪狂犬病病毒DNA[J]．中国生物化学与分子生物学报．2001，17(4)：519 523
[90] Scherba G, Jin L, Differential polymerasechain reaction for detection ofwild-type and vaccine strain of Aujeszky's disease(pseudorabies) virus[J].JVirol Methods, 1992, 38:131-144.
[91] Katz J B, Pedersen J C. Molecular analysis of pseudorabies virus vaccines and their rapid differentiation from wild type isolates using DNA amplified glycoprotein andthymidine kinase gene segment polymorphism[J].Biological, 1992,20:187-195.
[92] 冉智光、童光志．孔令达应用复合多聚酶链反应快速鉴别伪狂犬病弱毒疫苗与野毒[J]．中国兽医杂志，1999，25(5)：3-4．
[93] Weigel PM, Hahn EC, Scherba G. Survival and immunization of raccoonsafter exposure to pseudorabies (Aujeszky's disease) virus gene-deleted vaccines. [J]Vet Microbiol. 2003 Mar 20;92(1-2):19-24.
[94] 周以凤、张净．伪狂犬病微量血清中和试验[J]．上海畜牧兽医通讯1988，3：20-21．
[95] 方六荣、陈焕春、何启盖．应用微量中和试验进行猪伪狂犬病血清学调查[J]．中国畜禽传染病．1998，20(3)：151-153
[96] 吴斌、陈焕春、方六荣等．乳胶凝集试验与血清中和试验检测猪伪狂犬病血清抗体的比较[J]．中国兽医科技，1997，27(5)：23 24
[97] van Oirschot JT, Kaashoek MJ, Maris-Veldhuis MA, Rijsewijk FA. Strains of bovine herpesvirus 1 that do not express an epitope on glycoprotein E in cell culture still induce antibodies that can be detected in a gE-blocking ELISA. [J]Vet Microbiol. 1999 Mar 1;65(2):103-113
[98] Morenkov OS, Fodor N, Fodor I. Indirect ELISAs based on recombinant and affinity -purified glycoprotein E of Aujeszky's disease virus to differentiate between vaccinated and infected animals. [J]Acta Vet Hung. 1999;47(1):137-150.
[99] Jonsson MK, Levi M, Ruden U, Wahren B. Minimal change in MSV-2 seroreactivity: A cross-sectional Swedish population study. [J]Scand J Infect Dis. 2006;38(5):357-365
[100] Graziano N, McGuire MJ, Roberson A, Adams C, Jiang H, Blute N. 2004 National Atrazine Occurrence Monitoring Program using the Abraxis ELISA method. Environ Sci Technol. 2006 Feb 15;40(4):1163-1171.
[101] H J Nauwynck、M B Pensaert. Progremmes for the eradication of Aujeszky' s disease virus (pseudorabies virus) in the member of the European Union. In: Aujeszky' s Disease O.I.E. Symposium Bangkok[J],Thailand, 30 June & 1 July 1994,55-66
[102] 代卓见．伪狂犬病病毒分子生物学研究进展[J]．国外兽医学-畜禽疾病，1996，17(1)：1-5
[103] 何启盖、陈焕春、吴斌．猪伪狂犬病及其免疫防治．畜禽重大疫病生物技术防制研究[A]．北京：中国农业大学出版社，1998．242-248
[104] Van Rooij E M, Haagmans B L, Glansbeek H L, et al. Vet Immunol Immunopathol, 2000,74(1-2):3752-3758
[105] 娄高明、郭万柱等．伪狂犬病病毒糖蛋白gP50基因的克隆和表达[A]．畜禽重大疫病生物技术防制研究．北京：中国农业大学出版社，1998．235-241
[106] 娄高明、郭万柱、汪铭书等．PRV闽A株BamHI片段克隆及其第7片段的鉴定[A]．畜禽重大疫病生物技术防制研究．北京：中国农业大学出版社，1998．203-207
[107] http://www. ncbi. nlm. nih. gov
[108] 肖明．生物信息学及其在病毒研究中的应用[J]．上海师范大学学报(自然科学版)，2003，32(3)，96-102
[109] ZHANG G H, Ding D F. Search and Databank establishment[J].Acta of Biochemistry and Biophysics, 1995,27(4):367-373
[110] 王槐春．蛋白质与核酸序列分析的基础[M]．北京：人民军队出版社，1994
[111] HENIKOFFS, HEHIKOFF J C. Amino acid substitution matrices protein blocks[J].Proc Natl Acad Sci USA, 1992,89:10915—10919.
[112] HENIKOFF J C, PIETROKOVSKI S, HERIKOFFS. Recent enhancements the Blocks Database servers[J]. Nucleic Acids Research, 1997,25(1):222—225.
[113] BARROCH A, BUCHER P, HAFMAMK. The PROSITE databases, its Etatus in 1997[J]. Nucleic Acids Research. 1997,25(1):217—221.
[114] NEVLL-MANNING C G, WU T D, BRUTLAG D L. Highly specific protein sequence motifs for genomes analysis[J]. Proc. Natl. Acad. Sci. USA, 1998, 95:5865—5871.
[115] TATUSOV R L, ALTSCHUIL S F, KOONIN E V. Detection of conserved segments in protein: Iterative scanning of sequence database with alignment blocks [J]. Proc.Natl. Acad. Sci. USA, 1994, 91:12091—12095.
[116] ITHIMURE D. DNA analysis: New kids on the Block[J]. Science, 1999, 285:355—356.
[117]STRAUSS J H, STRAUSS E G. Viral RNA replication: with a little from the host[J]. Science, 1999,283:802—804.
[118] CARDON L R, STOROMO G D. Expectation maximization algorithm for identifying protern-binding sites with variable lengths from unaligned DNA fragments [J]. J. Mol.Biol .,1992,223:159-170
[119]GRIBSK0W M.MCLACHLAN A D. EISERBERG D. Profile analysis detection of distantly related protein[J]. Proc Natl Acad Sci USA, 1987, 84:4355-4358
[120] SCHNEIDER T D, SIORMO G D, GOLD L, et al. Information content of binding sites or nucleotide sequences[J]. J.Mol. Biol, 1986,188:415-431.
[121] BERC D G, VON HIPPEL P. H, Selection of DNA binding sites by regulatory proteins: statistical: statistical-mechanical theory and application to operation and promotes [J]. J.Mol. Biol, 1987,193:723—750.
[122]ST0RM0 G D, HARTZELL II G W. Identifying protein-binding sites from unaligned DNA fagments[J]. Proc Natl Acad Sci USA, 1989,86:1183-1187
[123] STORMO G D, FIELDS D S. Specificity, free energy and information content protein DNA interactions[J]. Trends in Biochemical Sciences, 1998, 23:109-113
[124]FIELDS D S, HE YI-YUAN, AL-UZRI A Y, et al. Quantitative specificity of the Mnt repressor[J]. J Mol Biol,1997,271:178-194
[125]GELFAND M S, KOONIN E V, MIRONOV A A. Predication of transcription regulatory sites in Archace by a comparative genomic approach[J]. Nucleic Acids Research, 2000, 28(3):695 -705
[126]MEWES H W, ALBERMANNK, BAHR M, et al. Overview of the yeast genome[J]. Nature, 1997, 387 (6632suppl):7-65
[127]ROTA P A, OBERSTE M S,MONROE S S, et al. Characteraction of a novel cononavirus associated with severeacute respiratory syndrome[J].Science, 2003,300:1394-1398
[128] MARRA M A, JONES S J M, ASTELL C R. et al. The genome sequence of the sars-associated coronavirus[J].Science, 2003,300:1399-1404
[129] 马相如，肖少波，方六榮，隙焕春．伪狂犬病病毒基因编码區堿基组成与密码子使用偏差[J]Acta Genetica Sinica，2005，32，6：616-624
[130] 姚卫东，田荣福，于国明．猪伪狂犬病病毒的分子生物学进展[J]．黑龙江畜牧兽医，2004．4：44-45
[2] Wittmann G. and Rziha, H.J. Herpesvrius diseases of cattle, horse, and pigs. In Developments in Veterinary Virology. Boston: Kluwer Academic Publishers, pp. 1989, 230-325
[3] 程道祥．伪狂犬病的感染传播与流行．[J]福建畜牧兽医．1999，21(1)：34-35
[4] 郭万柱、刘亚刚、娄高明．兽医病毒学[M]．成都：四川科学技术出版社，2003．140-142
[5] 吴清民．兽医传染病[M]．北京：中国农业大学出版社，2002．124-130
[6] 李三星．关于伪狂犬病．[J]河北农业大学学报，1989．12(1)：145-150．
[7] Beran G W, Davies E B, Arambulo P V, et aLPersistence of pseudorabies v″vus in infected swine. [J]J Am Vet Med Assoc, 1979, 176: 998-1001.
[8] Sabo A, Itajcani L. Latent pseudorabies infection in pigs. [J]Acta Virol, 1976, 20: 208-214.
[9] Rziha H-J, Mettenleiter C T, Ohlinger V, et al. Herpesvirus(pseudorabies vinrs)latency in swine: occurrence and physical state of viral DNA in neural tissues. [J]Virol, 1986, 155: 600-613.
[10] Belak S, Ballagi-Pordancy A, Fhsburg J, et al. Detection of pseudorabies virus DNA sequences by the polymaase chain reaction. [J]Arch Virol. 1989, 108: 279-286
[11] 蔡宝祥．家畜传染病学[M]．北京：中国农业出版社，1996，207-209
[12] 郭万柱、颜其贵．伪狂犬病预防控制研究进展．畜禽重大疫病生物技术防制研究[A]．北京：中国农业大学出版社，1998．156-166
[13] 陈斌。伪狂犬病病毒Fa株胸苷激酶基因克隆及PCR扩增诊断伪狂犬病病毒的研究[D]．雅安，四川农业大学，1996
[14] 童光玉等．猪伪狂犬病的诊断和防治，[J]中国兽医杂志，1993，19(8)：18-19
[15] 李树春等．仔猪伪狂犬病的诊断．[J]中国兽医杂志，1995，8，21
[16] 韦华姜等．湖北首次暴发猪伪狂犬病的报告．[J]中国畜禽传染病，1994，4，36-38
[17] 朱庆虎等．近10年我国伪狂犬病研究进展．[J]中国畜禽传染病，1996，6，59-61
[18]．刘正飞．伪狂犬病TK-／gE基因缺失疫苗研究．[D]．武汉：华中农业大学，2002
[19] 李成，刘树森，王继科，姜绍德，谷守林，郝桂玉，张坦．畜禽病毒图谱[M]．北京农业出版社，1987．15-16
[20] 郭万柱、吴彤、陈瑶先．动物微生物学[M]．成都：四川科学技术出版社，2001．327-328
[21] 许雁峰，郭万柱，石谦等．猪伪狂犬病病毒gD基因的研究进展[J]四川畜牧兽医2005年7期，19-20
[22] McGeoch, D. J., M. A. Dalrymple, A. J. Davison, et al. The complete DNA sequence of the long unique region in the genome of herpes simplex virus type 1[J]. J. Gen. Virol. 1988. 69(7): 1531-1574
[23] Dolan, A., F. E. Jamieson, C. Cunningham, et al. The genome sequence of herpes simplex virus type 2[J]. J. Virol. 1998.72(3): 2010-2021.
[24] Davison, A. J., and J. E. Scott. The complete DNA sequence of varicella-zoster virus[J]. J. Gen. Virol. 1986, 67(9): 1759-1816.
[25] Telford, E. A., M. S. Watson, K. McBride, et al. The DNA sequence of equine herpesvirus -1[J]. Virology 1992, 189(1): 304-316.
[26] Telford, E. A., M. S. Watson, J. Perry, et al. The DNA sequence of equine herpesvirus -4[J]. J. Gen. Virol. 1998, 79(5): 1197-1203.
[27] Tulman, E. R., C. L. Afonso, Z. Lu, et al. The genome of a very virulent Marek's disease virus[J]. J. Virol. 2000, 74(17): 7980-7988.
[28] Afonso, C. L., E. R. Tulman, Z. Lu, et al. The genome of turkey herpesvirus[J]. J. Virol. 2001, 75(2): 971-978.
[29] Barbara G. Klupp, Christoph J. Hengartner, Complete, Annotated Sequence of the Pseudorabies Virus Genome[J]. Journal of Virology, 2004(1): 424-440.
[30] 陈世界、郭万柱．伪狂犬病病毒分子生物学．畜禽重大疫病生物技术防制研究[A]．北京：中国农业大学出版社，1998．167-172
[31] Glenn F. Rall, Sabina Kupershmidt et al, Functions of the sequences at the ends of the inverted repeats of pseudorabies virus[J], Journal of Virology, Mar 1992: 1506-1519
[32] 余树民、郭万柱．伪狂犬病基因工程疫苗的研究现状．畜禽重大疫病生物技术防制研究[A]．北京：中国农业大学出版社，1998．179-184
[33] 王勤．伪狂犬病病毒Fa株gE基因的克隆与序列的比较分析[D]．雅安，四川农业大学，2000
[34] Menttenleiter. T.C 1991. Molecular biology of pseudorabies (Aujeszky's disease) virus[J]．Comp Immunol Microbiol Infect Dis 14：11-163
[35] 马相如．伪狂犬病病毒宿主关闭蛋白的基因克隆、表达及其结构分析[D]．武汉，华中农业大学，2005
[36] Mayra E M Campbell et al, DNA sequences which regulate the expression of pseudorabies virus major immediate early gene[J]. Virolgy(Vol157)1994, 307-326
[37] K L Glazenburg, B. P. H. Peeters et al, Construction and properties of pseudorabies virus recombinants with altered control of immediate early gene expression[J], Journal of virology, jane 1995, 189-197
[38] Suzette A, Priola Jack G Stevers. The 5' and 3' limits of transcription in the pseudorabies Virus latency associated transcription unit[J]. Virology, 1991, 182: 852-856
[39] Andrew K Cheung et al. Pseudorabies virus deletion mutants involving the EPO and LLT genes[J], U S Patent, Oct 1994, No. 5352596
[40] G ibsonW. St ructurea nda ssemblyo fth ev irion[J]. In tervirology, 1996, 39: 389-400
[41] F uchsW, K luppB G, G ranzowH, H engartnerC, Brackh, M undtA, E nquistL W, M etenleiterT C. Ph y sic alin teraction between envelop glycoproteinE and M of ps eudorabiesv irusand the major tegument protein UL49[J]. J Virol, 2002c, 76(16): 8208-8217
[42] G ershonh A, S hermanD L, Z huZ, G abelC A, A mbronR T, G ershonM D. Intracellular transport of newly synthesized varieellazoster virus: final envelopment in the trans-Golgi network[J]. J Virol, 2000, 68: 6372-6390
[43] Newcomb W N, Homa F L, Thomsen D R. Assembly of the herpes simplex virus procapsid from purified components and identification of small complexes containing the major capsid and scaffolding proteins[J]. J Virol, 1999, 73: 4239-425
[44] ZhouZ, Chen D, Jakana J. Visualization of tegument-capsid interactions and DNA in intact herpes simplex virus type Ⅰ virions[J]. J Virol, 1999, 73: 3210-3218
[45] 娄高明，杜伟贤．郭万柱伪狂犬病病毒糖蛋白研究进展[J]．广东畜牧兽医科技1999，24(4)：3-6
[46] 陈斌．伪狂犬病病毒及其免役研究进展(上)[J]．四川畜牧兽医2001，28(9)：32-34
[47] Takad A Kida H. Induction of protective antibody responwew against pseudorabies virus buintranasal vaccination with glycoproteinB inmice[J]. Arch-virol, 1995, 140(9): 1629-1635
[48] 张志，范伟兴．赵宏坤伪狂犬病病毒囊膜糖蛋白的研究进展[J]．山东农业大学学报(自然科学版)，2001，32(1)：85-89
[49] 范伟兴、赵宏坤．伪狂犬病病毒分子生物学研究新近展．中国畜牧兽医学会家畜传染病学分会第九次学术讨论会[A]．2001
[50] 王勤．猪伪狂犬病病毒gE基因的研究进展[J]．畜牧与兽医．2002，34(10)：42-44
[51] 宋耀彬．猪的伪狂犬病[J]．辽宁畜牧兽医．1982，1：35-38
[52] 阿都阿且等．家畜伪狂犬病的调查[J]．中国畜禽传染病．1992，63(2)，29-30
[53] 黄寿森等．牛伪狂犬病的研究(第一报)：牛伪狂犬病病毒的分离及若干生物学特性的研究[J]．畜牧兽医学报．1966，9(1)，22-29
[54] 倪杉．陕A株伪狂犬病病毒的分离与鉴定[J]．兽医科技资料．1979，2，12-18
[55] 佘柏荣等．广东吴川株伪狂犬病毒的鉴定[J]．中国兽送杂志．1986，12(7)，17-19
[56] 马崇文等．家畜伪狂犬病流行病学调查[J]．家畜传染病．1986，1，40-41
[57] 王永贤等．伪狂犬病毒YN株的分离与鉴定[J]．中国人民解放军兽医大学学报．1984，4(4)，317-321
[58] 韦贤金．耕牛伪狂犬病的诊断与防制[J]．广西兽医科技．1985，2，48-49
[59] 毕远龙．云南耕牛伪狂犬病病毒的理化特性与电镜观察[J]．家畜传染病．1986，4，44-46
[60] 李秉机．奶牛、绵羊伪狂犬病的初步观察[J]．中国兽医杂志．1983，6，21
[61] 叶公权．耕牛伪狂犬病病例的报告[J]．畜牧兽医科技(广东)．1987，3，29-32
[62] 肖裕波．疑似绵羊的伪狂犬病[J]．中国兽医杂志．1965，3(5)
[63] 王永洪等．一次猪、狗、猫伪狂犬病爆发流行的研究[J]，家畜传染病，19842，30-32
[64] 李成卓．马、鹿伪狂犬病的一次爆发[J]．中国兽医杂志．1983，4，22-23
[65] 佘永建．猪伪狂犬病[J]．畜牧与兽医．1987(1)：29-33
[66] 徐志文．四川省伪狂犬病分子流行病学研究[D]．雅安，四川农业大学，1998
[67] 何启盖、陈焕春．猪伪狂犬病新的流行病学特征[J]．华中农业大学学报2000增刊．总第33期
[68] 何启盖、刘正飞．猪伪狂犬病的防制研究进展[J]．河南畜牧兽医．2000，21(6)：9-11
[69] 唐万勇．猪伪狂犬病的病理学研究[D]．武汉：华中农业大学，2003
[70] Narita M, Inui S, Schimizu YTonsillar changes in pigs given pseudorabies (Aujeszky's disease) virus[J]. Am J Vet Res. 1984, 45(2): 247-251
[71] Kluge J P and Mare C J. Gross and microscopic lesions of prenatal and neonatal pseudorabies(Aujeszky's disease)virus in swine[J]. Proc Int Congro Pig Vet Soc 1976，4：63．
[72] 张斌、汪开毓、冯泽光、郭万柱．仔猪伪狂犬病病理学研究[J]．畜牧兽医学报．1992，23(2)：182-186
[73] Narita M ,Kubo M, FukushoA, Haritani M, Moriwaki M. Necrotizing enteritis in piglets associated with the virus of Aujeszky's disease[J].Veterinary Pathol. 1984.21:450-452.
[74] 蒋鹤春．伪狂犬病病毒gE基因的表达及初步应用[D]．南京：南京农业大学，2000
[75] 刘镇明、蓝天．猪伪狂犬病病毒粤株的分离与鉴定[J]．华南农业大学学报．2000，(21)2：76-78．
[76] 祁贤、张婉华．猪伪狂犬病病毒S1株的分离与鉴定．Cninese Journal of Veterinary Science and Technology [J].2002,132(4):24-26
[77] Gutekunst DE, Pirtle EC, Miller LD, Stewart WC.. Isolation of pseudorabies virus from trigeminal ganglia of a latently infected sow[J].Am J Vet Res. 1980 Aug;41(8):1315-1316
[78] Dangler CA, Henderson LM, Bowman LA, Dearer RE. Direct isolation and identification of recombinant pseudorabies virus strains from tissues of experimentally co-infected swine. Am J Vet Res. 1993 Apr;54(4):540-5.
[79] 吴雅玲．猪伪狂犬病[J]．青海畜牧兽医杂志．2001，31(2)：34-35
[80] 程由铨、吴平、林天龙等．4种检测伪狂犬病病毒抗原方法的的比较[J]．中国兽医杂志．1992，18(1)：6-9．
[81] 娄高明，陈志荣，郭万柱等．双抗体夹心ELISA检测伪狂犬病病毒的研究[J]．中国畜禽传染病，1998，20(4)：236～240
[82] 郭万柱、冯炳芳、曹军等．应用~(32)P标记DNA探针检测伪狂犬病病病毒的研究[J]．四川农业大学报．1991，9(1)：52 56
[83] 王琴、郭万柱．应用生物素标记DNA探针检测伪狂犬病病毒的研究[J]．中国病毒学．1996，(11)3：284-285
[84] 李学伍、陈焕春、周复春等．用地高辛标记的DNA探针检测伪狂犬病毒[J]．畜牧与兽医．1997，29(6)：270-271
[85] Maes R K, Sussman M D, Recent. Developments in Iatency and recombination of Aujeszky's disease (pseudorabies) virus[J]. Vet Microbiol. 1997,55:13-27.
[86] J. Galeota Wheeler, BA Investigation of sites of Pseudorabies virus latency using polymerase chain reaction[J]Am vet Res, 1991,52(11):1799-1803
[87] 周复春、陈焕春．伪狂犬病病毒PCR检测方法的建立[J]．华中农业大学学报．2000年增刊总第33期
[88] 冉多良、王正党、魏伟应．用聚合酶联反应检测伪狂犬病毒DNA[J]．中国兽医学报1994，14(4)：366-368
[89] 娄高明，杜伟贤，廖筱萍．PCR检测伪狂犬病病毒DNA[J]．中国生物化学与分子生物学报．2001，17(4)：519 523
[90] Scherba G, Jin L, Differential polymerasechain reaction for detection ofwild-type and vaccine strain of Aujeszky's disease(pseudorabies) virus[J].JVirol Methods, 1992, 38:131-144.
[91] Katz J B, Pedersen J C. Molecular analysis of pseudorabies virus vaccines and their rapid differentiation from wild type isolates using DNA amplified glycoprotein andthymidine kinase gene segment polymorphism[J].Biological, 1992,20:187-195.
[92] 冉智光、童光志．孔令达应用复合多聚酶链反应快速鉴别伪狂犬病弱毒疫苗与野毒[J]．中国兽医杂志，1999，25(5)：3-4．
[93] Weigel PM, Hahn EC, Scherba G. Survival and immunization of raccoonsafter exposure to pseudorabies (Aujeszky's disease) virus gene-deleted vaccines. [J]Vet Microbiol. 2003 Mar 20;92(1-2):19-24.
[94] 周以凤、张净．伪狂犬病微量血清中和试验[J]．上海畜牧兽医通讯1988，3：20-21．
[95] 方六荣、陈焕春、何启盖．应用微量中和试验进行猪伪狂犬病血清学调查[J]．中国畜禽传染病．1998，20(3)：151-153
[96] 吴斌、陈焕春、方六荣等．乳胶凝集试验与血清中和试验检测猪伪狂犬病血清抗体的比较[J]．中国兽医科技，1997，27(5)：23 24
[97] van Oirschot JT, Kaashoek MJ, Maris-Veldhuis MA, Rijsewijk FA. Strains of bovine herpesvirus 1 that do not express an epitope on glycoprotein E in cell culture still induce antibodies that can be detected in a gE-blocking ELISA. [J]Vet Microbiol. 1999 Mar 1;65(2):103-113
[98] Morenkov OS, Fodor N, Fodor I. Indirect ELISAs based on recombinant and affinity -purified glycoprotein E of Aujeszky's disease virus to differentiate between vaccinated and infected animals. [J]Acta Vet Hung. 1999;47(1):137-150.
[99] Jonsson MK, Levi M, Ruden U, Wahren B. Minimal change in MSV-2 seroreactivity: A cross-sectional Swedish population study. [J]Scand J Infect Dis. 2006;38(5):357-365
[100] Graziano N, McGuire MJ, Roberson A, Adams C, Jiang H, Blute N. 2004 National Atrazine Occurrence Monitoring Program using the Abraxis ELISA method. Environ Sci Technol. 2006 Feb 15;40(4):1163-1171.
[101] H J Nauwynck、M B Pensaert. Progremmes for the eradication of Aujeszky' s disease virus (pseudorabies virus) in the member of the European Union. In: Aujeszky' s Disease O.I.E. Symposium Bangkok[J],Thailand, 30 June & 1 July 1994,55-66
[102] 代卓见．伪狂犬病病毒分子生物学研究进展[J]．国外兽医学-畜禽疾病，1996，17(1)：1-5
[103] 何启盖、陈焕春、吴斌．猪伪狂犬病及其免疫防治．畜禽重大疫病生物技术防制研究[A]．北京：中国农业大学出版社，1998．242-248
[104] Van Rooij E M, Haagmans B L, Glansbeek H L, et al. Vet Immunol Immunopathol, 2000,74(1-2):3752-3758
[105] 娄高明、郭万柱等．伪狂犬病病毒糖蛋白gP50基因的克隆和表达[A]．畜禽重大疫病生物技术防制研究．北京：中国农业大学出版社，1998．235-241
[106] 娄高明、郭万柱、汪铭书等．PRV闽A株BamHI片段克隆及其第7片段的鉴定[A]．畜禽重大疫病生物技术防制研究．北京：中国农业大学出版社，1998．203-207
[107] http://www. ncbi. nlm. nih. gov
[108] 肖明．生物信息学及其在病毒研究中的应用[J]．上海师范大学学报(自然科学版)，2003，32(3)，96-102
[109] ZHANG G H, Ding D F. Search and Databank establishment[J].Acta of Biochemistry and Biophysics, 1995,27(4):367-373
[110] 王槐春．蛋白质与核酸序列分析的基础[M]．北京：人民军队出版社，1994
[111] HENIKOFFS, HEHIKOFF J C. Amino acid substitution matrices protein blocks[J].Proc Natl Acad Sci USA, 1992,89:10915—10919.
[112] HENIKOFF J C, PIETROKOVSKI S, HERIKOFFS. Recent enhancements the Blocks Database servers[J]. Nucleic Acids Research, 1997,25(1):222—225.
[113] BARROCH A, BUCHER P, HAFMAMK. The PROSITE databases, its Etatus in 1997[J]. Nucleic Acids Research. 1997,25(1):217—221.
[114] NEVLL-MANNING C G, WU T D, BRUTLAG D L. Highly specific protein sequence motifs for genomes analysis[J]. Proc. Natl. Acad. Sci. USA, 1998, 95:5865—5871.
[115] TATUSOV R L, ALTSCHUIL S F, KOONIN E V. Detection of conserved segments in protein: Iterative scanning of sequence database with alignment blocks [J]. Proc.Natl. Acad. Sci. USA, 1994, 91:12091—12095.
[116] ITHIMURE D. DNA analysis: New kids on the Block[J]. Science, 1999, 285:355—356.
[117]STRAUSS J H, STRAUSS E G. Viral RNA replication: with a little from the host[J]. Science, 1999,283:802—804.
[118] CARDON L R, STOROMO G D. Expectation maximization algorithm for identifying protern-binding sites with variable lengths from unaligned DNA fragments [J]. J. Mol.Biol .,1992,223:159-170
[119]GRIBSK0W M.MCLACHLAN A D. EISERBERG D. Profile analysis detection of distantly related protein[J]. Proc Natl Acad Sci USA, 1987, 84:4355-4358
[120] SCHNEIDER T D, SIORMO G D, GOLD L, et al. Information content of binding sites or nucleotide sequences[J]. J.Mol. Biol, 1986,188:415-431.
[121] BERC D G, VON HIPPEL P. H, Selection of DNA binding sites by regulatory proteins: statistical: statistical-mechanical theory and application to operation and promotes [J]. J.Mol. Biol, 1987,193:723—750.
[122]ST0RM0 G D, HARTZELL II G W. Identifying protein-binding sites from unaligned DNA fagments[J]. Proc Natl Acad Sci USA, 1989,86:1183-1187
[123] STORMO G D, FIELDS D S. Specificity, free energy and information content protein DNA interactions[J]. Trends in Biochemical Sciences, 1998, 23:109-113
[124]FIELDS D S, HE YI-YUAN, AL-UZRI A Y, et al. Quantitative specificity of the Mnt repressor[J]. J Mol Biol,1997,271:178-194
[125]GELFAND M S, KOONIN E V, MIRONOV A A. Predication of transcription regulatory sites in Archace by a comparative genomic approach[J]. Nucleic Acids Research, 2000, 28(3):695 -705
[126]MEWES H W, ALBERMANNK, BAHR M, et al. Overview of the yeast genome[J]. Nature, 1997, 387 (6632suppl):7-65
[127]ROTA P A, OBERSTE M S,MONROE S S, et al. Characteraction of a novel cononavirus associated with severeacute respiratory syndrome[J].Science, 2003,300:1394-1398
[128] MARRA M A, JONES S J M, ASTELL C R. et al. The genome sequence of the sars-associated coronavirus[J].Science, 2003,300:1399-1404
[129] 马相如，肖少波，方六榮，隙焕春．伪狂犬病病毒基因编码區堿基组成与密码子使用偏差[J]Acta Genetica Sinica，2005，32，6：616-624
[130] 姚卫东，田荣福，于国明．猪伪狂犬病病毒的分子生物学进展[J]．黑龙江畜牧兽医，2004．4：44-45