鸭瘟病毒基因组脉冲场电泳分析和UL41突变体的构建
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摘要
将DEV疫苗株在CEF上增殖培养获得高滴度的病毒液,提取鸭瘟病毒基因组DNA进行限制性酶切,对酶切产物经脉冲场电泳分析,结果表明DEV全基因组长度位于145-194 kb之间,经限制性酶切后脉冲场电泳图谱条带清晰。
分别以MOI 1和MOI0.01病毒液接种次代CEF,研究DEV在细胞上能生长的滴度和生长复制的特性,结果表明病毒接种CEF 48小时可使半数的CEF感染,病毒的TCID50为10-7.55,同时病毒在CEF上具有很好的生长复制,为研究和评价DEV基因工程疫苗效价提供依据。
利用Red重组技术在已构建好的DEV多粘粒感染性克隆的粘粒上构建DEVUL41突变,把获得的突变体电泳鉴定并转染CEF,构建好的UL41突变体在CEF上很好的表达了蛋白,结果表明成功的构建了UL41突变体,同时由于UL41的突变并没有影响蛋白的表达,说明UL41是DEV的非必需基因。
After propagating in chicken embryo fibroblast (CEF), high titer Duck Enteritis Virus (C-KCE strain) was collected to extract viral DNA, then the viral DNA abtained was degested by restriction enzyme. CHEF MAPPER was employed to analysis digested products, and results showed the whole DEV genome located between 145kb-194kb and the DNA sample was highly purified and ready to be used for genomic library construction.
CEF was infected by DEV with dilution of MOI 1 and MOI 0.01 to study virus titer in the cell culture fluid and its growth and replication status, as the results showed DEV could growth well in CEP and TCID50 was 10-7.55 post infection 48h.
According to the UL41 genome of DEV, the specific primers were designed to amplify UL41 genome by PCR, and then the UL41 Mutant was amplified by PCR used mutation primer. UL41 Mutant DEV was constructed successfully with Red homologous-recombination to transfect the CEP, and the results of immunofluorescence assay dicated that UL41 Mutant DEV can proliferate and express proteins normally. This indicated that UL41 is not essential for virus replication.
分别以MOI 1和MOI0.01病毒液接种次代CEF,研究DEV在细胞上能生长的滴度和生长复制的特性,结果表明病毒接种CEF 48小时可使半数的CEF感染,病毒的TCID50为10-7.55,同时病毒在CEF上具有很好的生长复制,为研究和评价DEV基因工程疫苗效价提供依据。
利用Red重组技术在已构建好的DEV多粘粒感染性克隆的粘粒上构建DEVUL41突变,把获得的突变体电泳鉴定并转染CEF,构建好的UL41突变体在CEF上很好的表达了蛋白,结果表明成功的构建了UL41突变体,同时由于UL41的突变并没有影响蛋白的表达,说明UL41是DEV的非必需基因。
After propagating in chicken embryo fibroblast (CEF), high titer Duck Enteritis Virus (C-KCE strain) was collected to extract viral DNA, then the viral DNA abtained was degested by restriction enzyme. CHEF MAPPER was employed to analysis digested products, and results showed the whole DEV genome located between 145kb-194kb and the DNA sample was highly purified and ready to be used for genomic library construction.
CEF was infected by DEV with dilution of MOI 1 and MOI 0.01 to study virus titer in the cell culture fluid and its growth and replication status, as the results showed DEV could growth well in CEP and TCID50 was 10-7.55 post infection 48h.
According to the UL41 genome of DEV, the specific primers were designed to amplify UL41 genome by PCR, and then the UL41 Mutant was amplified by PCR used mutation primer. UL41 Mutant DEV was constructed successfully with Red homologous-recombination to transfect the CEP, and the results of immunofluorescence assay dicated that UL41 Mutant DEV can proliferate and express proteins normally. This indicated that UL41 is not essential for virus replication.
引文
[1]黄引贤,欧守抒,邝荣禄.鸭瘟病毒的研究[J].华南农学院学报,1980,1(1)：21-33.
[2]殷震,刘景华.动物病毒学[M].第二版.北京.1999,科学技术出版社.
[3]陈天杰,赵长旺,姚月华,等.广东省农科院石井系鸭瘟弱毒疫苗对我国台湾“花鸭”澳洲“狄高”鸭和丹麦白鸭的安效试验[J].广东畜牧兽医科技.1982(01)：21-23.
[4]郭宇飞,程安春,汪铭书,等.鸭胚成纤维细胞中鸭瘟强毒的增殖特性研究[J].病毒学报,2008,(05)：18-21.
[5]Field, B. N, Knipe, D.M, and Howley, P.M. " Fiends virogy. " Lippincott W illiams & Wilkins.2007.
[6]Yu-feng, L., of Agricultural, Sciences, B. H. F. L. Y.-s. L. X.-1. M. J. W. M.-x. S. H.-c. Y. P. I. o. S. A., Jinan, China.2. Preventive Veterinary Medicine Key Laboratory of Ministry of, A., China Agricultural, U., Beijing, and China. Sequence analysis of duck enteritis virus genome.2007, (09):123-128.
[7]Gardner R W J, Johnson J C. Molecular characterization of the DNA of Anatid herpesvirus[J]. Intervirology,1993,36(2):99-112.
[8]Plummer, P. J., Alefantis, T., Kaplan, S.,0'Connell, P., Shawky, S., Schat, K. A.,1998. Detection of duck enteritis virus by polymerase chain reaction. Avian Dis.42,554-564.
[9]Hansen, W. R., Brown, S. E., Nashold, S. W., Knudson, D. L.,1999. Identification of duck plague virus by polymerase chain reaction. Avian Dis. 43,106-115.
[10]Yufeng Li, Hanchun Yang., et al., Molecular characterization of the genome of duck enteritis virus[J]. Virology. virol.2009,6(18):1-11.
[11]张晓根,王欣,崔世新.鹅的鸭瘟病原分离鉴定及治疗试验.中国兽医杂志2001,(37)：14-15.
[12]文明,罗永荣,袁翠霞,等.一起鹅群发生鸭瘟病例的实验室诊断[J].养禽与禽病防治.2007,(8)：40-42.
[13]陈普成,曾青华,柳金雄,等。鸭病毒性肠炎病毒UL41、UL42基因的克隆与分析。预防兽医学报中国预防兽医学报,2009,(05)：479-693.
[14]卢杏通,廖明,郭霄峰,赵明秋,刘穗,.谢淑敏,辛朝安.鸭瘟病毒基因组核酸的制备.中国兽医科技2003,(04)：49-51
[15]马相如,肖少波.VHS-蛋白一种具有mRNA剪切活性的多种功能蛋白质2005.25(2)：100-103.
[16]Herold, B. C., WuDunn, D., Soltys, N., et al.. Glycoprotein C of herpes simplex virus type 1 plays a principal role in the adsorption of virus to cells and in infectivity[J]. J Virol 1991,65(3):1090-1098.
[17]Seidel-Dugan, C., Ponce de Leon, M., Friedman, H. M., Eisenberg, R. J. and Cohen, G. H. Identification of C3b-binding regions on herpes simplex virus type 2 glycoprotein C. J Virol 1990,64(5):1897-1906.
[18]McGeoch, D. J., Dalrymple, M.A., Davison, A. J., Dolan, A., Frame, M. C., McNab, D., Perry, L. J., Scott, J. E., Taylor, P.,1988. The complete DNA sequence of the long unique region in the genome of herpes simplex virus type 1. J. Gen. Virol.69,1531-1537.
[19]Cocchi, F., Fusco, D., Menotti, L., et al.. The soluble ectodomain of herpes simplex virus gD contains a membrane-proximal pro-fusion domain and suffices to mediate virus entry[J]. Proc Natl Acad Sci USA.2004,101(19): 7445-7450.
[20]Dingwell, K. S., Johnson, D. C. The herpes simplex virus gE-gI complex facilitates cell-to-cell spread and binds to components of cell junctions [J]. J Virol.1998,72(11):8933-8942.
[21]Shawky, S. A., Sandhu, T. S. Inactivated vaccine for protection against duck virus enteritis[J]. Avian Dis.1997,41(2),:461-468.
[22]Samady L, Costigliola E, MacCormac L, et al. Deletion of the virion host shutoff protein (vhs) from Herpes simplex virus (HSV) relieves the viral block to dendritic cell activation:potential of vhs-HSV vectors for dendritic cell-Mediated immunotherapy [J]. J Virol,2003,77 (6):3768-3776.
[23]Prechtel AT, Turza NM, Kobelt D J, et al. Infection of mature dendritic cells with Herpes simplex virus type 1 dramatically reduces lymphoid chemokine-mediated migration [J]. J Gen Virol,2005,86:1645-1657.
[24]Sullivan, V., and Smith, G. L. Expression and characterization of herpes simplex virus type 1 (HSV-1) glycoprotein G (gG) by recombinant vaccinia virus: neutralization of HSV-1 infectivity with anti-gG antibody[J]. J Gen Virol. 1987,68 (10):2587-2598.
[25]Chee A V, Roizman B. Herpes simplex virusl gene products occlude the interferon signaling pathway at multiple sites [J]. J Virol,2004,78 (8): 4185-4196.
[26]Dubin, G., Jiang, H. Expression of herpes simplex virus type 1 glycoprotein L (gL) in transfected mammalian cells:evidence that gL is not independently anchored to cell membranes [J]. J Virol.1995,69(7).4564-4568.
[27]Maclean S, Belmont A S. Sequential recruitment of HAT andSWI/SNF components to condensed chromatin by VP16 [J].CurrBiol,2003,13 (3):241-6.
[28]Karr, B. M., Read, G. S. The virion host shutoff function of herpes simplex virus degrades the 5' end of a target mRNA before the 3' end[J]. Virology 1999,264(1):195-204.
[29]A.D. Kwong, J.A. Kruper, N. Frenkel, J. Virol.62,912-921(1988). 2. P. Feng, D. N. Everly Jr, G. S. Read, J. Virol.79,9651-9664 (2005).
[30]Triezenberg, S. J., Kingsbury, R. C., and McKnight, S. L. Functional dissection of VP16, the trans-activator of herpes simplex virus immediate early gene expression. Genes Dev 1988.2(6),718-729.
[31]Wysocka J, Herr W. The Herpes simplex virus VP16-inducedcomplex:the makings of a regulatory switch [J]. Biochem Sci,2003,28 (6):294-304.
[32]郭灵,谢瑶,汪华侨,等.腺病毒载体介导的1—-e-Y.基因在神经前体细胞的转染和表达[J].神经解剖学杂志,2003,19(2)：135-138.
[33]罗薇,靳艳玲,刘内生,等.鸭瘟病毒CY07株诱导的细胞凋亡及凋亡对其增殖的影响[J].中国兽医科学.2009,39(07)：575-579.
[34]Desai, P., Sexton, G. L., Huang, E., et al.. Localization of herpes simplex virus type 1 UL37 in the Golgi complex requires UL36 but not capsid structures[J]. J Virol 2008,82(22):11354-11361.
[35]李慧昕.鸭肠炎病毒部分基因结构及相关功能的初步研究.[博士学位论文].北京：中国农业科学院,2007.
[36]Sheaffer, A. K., Newcomb, W. W., Brown, J. C., et al.. Evidence for controlled incorporation of herpes simplex virus type 1 UL26 protease into capsids[J]. J Virol.2000,74(15):6838-6848.
[37]Esclatine A, Taddeo B, Evans L, et al. The Herpes simplex virus 1UL41 gene-dependent destabilization of cellular RNAs is selectiveand may be sequence-specific [J]. PNAS,2004,101(10):3603-3608.
[38]Meckes, D. G., Jr., Wills, J. W. Dynamic interactions of the UL16 tegument protein with the capsid of herpes simplex virus[J]. J Virol.2007,81(23):13028-13036.
[39]Yokota S I, Yokosawa N, Okabayashi T, et al. Induction of suppressor of cytokine signaling-3 by Herpes simplex virus type 1 contributes to inhibition of the interferon signaling pathway [J]. JVirol,2004,78 (12):6282-6286
[40]Memedula S, Belmont A S. Sequential recruitment of HAT andSWI/SNF components to.condensed chromatin by VP16 [J]. CurrBiol,2003,13 (3):241-6.
[41]Wysocka, J., Herr, W. The herpes simplex virus VP16-induced complex: the makings of a regulatory switch. Trends Biochem[J] Sci.2003,28(6):294-304.
[42]Wysocka J, Herr W. The Herpes simplex virus VP16-inducedcomplex:the makings of a regulatory switch [J]. Biochem Sci,2003,28 (6):294-304.
[43]陈淑红,韩宗玺,邵昱昊,等.鸭肠炎病毒UL6基因的分子特征[J].病毒学报.2006,22(5)：391-396.
[44]Strube, W., Auer, S., Block, W., Heinen, E., Kretzdorn, D., Rodenbach, C., and Schmeer, N. A gE deleted infectious bovine rhinotracheitis marker vaccine for use in improved bovine herpesvirus 1 control programs. Vet Microbiol 1996.53(1-2),181-189.
[45]Sullivan, V., and Smith, G. L. Expression and characterization of herpes simplex virus type 1 (HSV-1) glycoprotein G (gG) by recombinant vaccinia virus: neutralization of HSV-1 infectivity with anti-gG antibody. J Gen Virol 1987.68 (Pt 10),2587-2598.
[46]Taddeo, B. R., B. The virion host shutoff protein (UL41) of herpes simplex virus 1 is an endoribonuclease with a substrate specificity similar to that of RNase A. J Virol 2006.80(18),9341-9345
[47]Burgess, E. C., and T. M. Yuill. Vertical transmission of duck plague virus by apparently healthy DPV carrier waterfowl[J].Avian Dis.1981(25):795 800.
[48]Sp ieker J 0, Yuill TM, Burgess E C. V irulence of six strains of duck p lague virus in eigh t waterfow 1 species [J]. Journal of W ildlife D iseases. 1996,32 (3):453-460.
[49]Tigges M A, Leng S, Johnson D C, et al. Human herpes simplex virus (HSV)-specific CD8+ CTL clones recognize HSV-2-infected fibroblasts after treatment with IFN-gamma or when virion host shutoff functions are disabled [J]. Immunol,1996,156:3901-3910.
[50]程安春,汪铭书等,现代禽病诊断和防治全书[M],成都,四川大学出版社,1997,32：505。
[51]尚世荣.鹅感染鸭瘟病毒的诊治报告 [J].畜牧兽医科技信息.2007,(12)：102-103.
[52]吕桂霞,刁有祥.“新型鸭瘟”流行病学及病原特性的研究.2003,山东农大学位论文.
[53]陆新浩,刘鸿,朱梦代等,一种新疫病在浙江的流行及防治初探[C],中国畜牧兽医学会禽病学分会第十一次学术研讨会论文集,2002 396 397
[54]童光志,王云峰,等.基因工程疫苗原理与方法[M].北京.2009,化学工业出版社.
[55]A.D. Kwong, N. Frenkel, Proc. Natl. Acad. Sci. USA 84,1926-1930 (1987).
[56]A. A. Oroskar, G. S. Read, J. Virol.61,604-606 (1987).
[57]T. L. Keadle, L. A. Morrison, J. L. Morris, J. S. Pepose, P.M. Stuart, J. Virol.76,3615-3625 (2002).
[58]T. J. Smith, L.A. Morrison, D. A. Leib, J. Virol.76,2054-2061(2002).
[59]R. L. Witter, K.A. Schat, in Diseases of Poultry, ed. by Y. M. Saif, H. J. Barnes, J. R. Glisson, A.M. Fadly, L. R. McDougald, D. E. Swayne (Iowa State Press, Ames,2003), pp.407-465.
[60]R.L. Witter, Avian Dis.41,149-163 (1997).
[61]L. D. Bacon, H. D. Hunt, H. H. Cheng, Poult. Sci.79,1082-1093(2000).
[62]乔代蓉.限制性内切酶分析鸭瘟病毒DNA.四川农业大学学报1992.10(001),172-177.
[63]李玉峰,何叶峰,黄兵.鸭瘟病毒基因组的提取和限制性酶切分析.中国家禽2007.(05).12-14.
[64]李子剑,李玉峰,陈苏,宋敏训,李云龙.鸭瘟病毒基因组核酸的提取方法比较.山东师范大学学报(自然科学版)2008.(01).134-136.
[65]J.萨姆布鲁克等著,黄培堂等译.分子克隆实验操作指南(第三版).北京：科学出版社.2002.
[66]Perez-Parada J, Saffran H A, Smiley J R. RNA Degradation induced by the Herpes simplex virus vhs protein proceeds 5' to 3' in vitro [J]. J Virol,2004, 78 (23):13391-13394.
[67]Q. Lam, C. A. Smibert, K. E. Koop, C. Lavery, J. P. Capone, S. P. Weinheimer, J. R. Smiley, EMBO J.15,2575-2581 (1996).
[68]B. Taddeo, M. T. Sciortino, W. Zhang, B. Roizman, Proc. Natl.Acad. Sci. USA 104,12163-12168 (2007).
[69]D. Koppers-Lalic, F. A. Rijsewijk, S. B. Verschuren, J.A. vanGaans-Van den Brink, A. Neisig, M. E. Ressing, J. Neefjes, E. J. Wiertz, J. Gen. Virol. 82,2071-2081 (2001).
[70]R. S. Gopinath, A. P. Ambagala, S. Hinkley, S. Srikumaran, ViralImmunol. 15,595-608 (2002).
[71]Jerome, K. R., Chen, Z., Lang, R., et al.. HSV and glycoprotein J inhibit caspase activation and apoptosis induced by granzyme B or Fas[J]. J Immunol. 2001,167(7):3928-3935.
[72]Baines, J. D., Wills, E., Jacob, R. J., et al.. Glycoprotein M of herpes simplex virus 1 is incorporated into virions during budding at the inner nuclear membrane[J]. J Virol.2007,81(2):800-812.
[73]ato C, Ohmiya R, Mizuno T. A rap id method for disrup tinggenes in the Escherichia coli genome. Biosci BiotechnolBiochem,1998,62:1826～1829
[74]Sheaffer, A. K., Newcomb, W. W., Brown, J. C., Gao, M., Weller, S. K., and Tenney, D. J. Evidence for controlled incorporation of herpes simplex virus type 1 UL26 protease into capsids. J Virol 2000.74(15),6838-6848.
[75]Smiley, J. R. Herpes simplex virus virion host shutoff protein:immune evasion mediated by a viral RNase? J Virol 2004.78(3),1063-1068.
[76]Sonoda, K., Sakaguchi, M., Okamura, H., Yokogawa, K., Tokunaga, E., Tokiyoshi, S., Kawaguchi, Y., and Hirai, K. Development of an effective polyvalent vaccine against both Marek's and Newcastle diseases based on recombinant Marek's disease virus type 1 in commercial chickens with maternal antibodies. J Virol 2000.74(7),3217-3226.
[77]Roop, C., Hutchinson, L., and Johnson, D. C. A mutant herpes simplex virus type 1 unable to express glycoprotein L cannot enter cells, and its particles lack glycoprotein H. J Virol 1993.67(4),2285-2297.
[78]Sakaguchi, M., Nakamura, H., Sonoda, K., Okamura, H., Yokogawa, K., .Matsuo, K., and Hira, K. Protection of chickens with or without maternal antibodies against both Marek's and Newcastle diseases by one-time vaccination with recombinant vaccine of Marek's disease virus type 1. Vaccine 1998.16(5), 472-479.
[79]Thomsen, D. R., Newcomb, W. W., Brown, J. C., and Homa, F. L. Assembly of the herpes simplex virus capsid:requirement for the carboxyl-terminal twenty-five amino acids of the proteins encoded by the UL26 and UL26.5 genes. J Virol 1995.69(6),3690-3703.
[80]Shawky, S. and Schat, K. A. Latency sites and reactivation of duck enteritis virus. Avian Dis 2002.46(2),308-313.
[81]Poteete A R, Fenton A C. Genetic requirements of phagelambda red2mediated gene rep lacement in Escherichia coli K212. J Bacteriol,2000,182:2336～ 2340
[82]Kuzminov A. Recombinational repair of DNA damage inEscherichia coli and bacteriophage lambda. MicrobiolMol BiolRev,1999,63:751～813
[83]Kovall R, Matthews B W. Toroidal structure of lambdaexonuclease. Science, 1997,277:1824-1827
[84]Kowalczykowski S C, Dixon D A, Rehrauer W M, et al.Biochemistry of homologous recombination in Escherichia coli. Microbiol Rev,1994,58:401～ 465
[85]Roca A I, CoxM M. RecA p rotein:structure, function, androle in recombinationalDNA repair. ProgNucleic Acid ResMolBiol,1997,56:129～223
[86]Kogoma T, Cadwell G W, Barnard K G, et al. The DNArep lication p riming p rotein, PriA, is required for homologousrecombination and double2strand break repair. J Bacteriol,1996,178:1258～1264
[87]Dykstra C C, Prasher D, Kushner S R. Physical andbiochemical analysis of the cloned recB and recC genes ofEscherichia coli K212. J Bacteriol,1984, 157:21-27
[88]Murphy K C. Use of bacteriophage 1 recombination functions top romote gene rep lacement in Escherichia coli. J. Bacteriol,1998,180:2063～2071
[89]Murphy K C, Campellone K G, Poteete A R. PCR2mediated gene rep lacement in Escherichia coli. Gene,2000,246:321～330
[90]Datsenko K A, Wanner B L. One2step inactivation of chromosomal genes in Escherichia coli K212 using PCR p roducts. Proc Natl Acad Sci USA,2000, 97: 6640-6645
[91]侯松旺,陈新文,王汉中,等1一种以PCR产物直接构建同源重组杆状病毒的方法1中国科学(C辑),2003,33(2)：169-174
[92]王恒梁,冯尔玲,史兆兴,等1Red系统快速敲除痢疾杆菌asd基因1军事医学科学院刊,2002,26(3)：161-164
[93]张全,高会杰,佟明友.Red重组技术研究进展.中国生物工程杂志.2006,26(1)：81-86.
[94]Strand S S, Leib D A. Role of the VP16-Binding domain of VHS inviral growth, host shutoff activity, and pathogenesis [J]. J Virol,2004,78 (24): 13562-13572.
[95]Feng P, Everly D N, Read G S. mRNA Decay during Herpes simplexvirus (HSV) infections:protein-protein interactions involving theHSV virion host shutoff protein and translation factors eIF4H andeIF4A [J]. Virology,2005,79(15): 9651-9664.
[96]李子剑.鸭肠炎病毒基因组复制非必需区的筛选.[硕士学位论文].济南：山 东师范大学,2008
[97]Geiss B J, et al. J Virol,2000,74:11137-11144.
[98]Hinkley S, Ambagala A P N, Jones C J, et al. A vhs-like activity of Bovine herpesvirus-1 [J]. Arch Virol,2000,145:2027-2046.
[2]殷震,刘景华.动物病毒学[M].第二版.北京.1999,科学技术出版社.
[3]陈天杰,赵长旺,姚月华,等.广东省农科院石井系鸭瘟弱毒疫苗对我国台湾“花鸭”澳洲“狄高”鸭和丹麦白鸭的安效试验[J].广东畜牧兽医科技.1982(01)：21-23.
[4]郭宇飞,程安春,汪铭书,等.鸭胚成纤维细胞中鸭瘟强毒的增殖特性研究[J].病毒学报,2008,(05)：18-21.
[5]Field, B. N, Knipe, D.M, and Howley, P.M. " Fiends virogy. " Lippincott W illiams & Wilkins.2007.
[6]Yu-feng, L., of Agricultural, Sciences, B. H. F. L. Y.-s. L. X.-1. M. J. W. M.-x. S. H.-c. Y. P. I. o. S. A., Jinan, China.2. Preventive Veterinary Medicine Key Laboratory of Ministry of, A., China Agricultural, U., Beijing, and China. Sequence analysis of duck enteritis virus genome.2007, (09):123-128.
[7]Gardner R W J, Johnson J C. Molecular characterization of the DNA of Anatid herpesvirus[J]. Intervirology,1993,36(2):99-112.
[8]Plummer, P. J., Alefantis, T., Kaplan, S.,0'Connell, P., Shawky, S., Schat, K. A.,1998. Detection of duck enteritis virus by polymerase chain reaction. Avian Dis.42,554-564.
[9]Hansen, W. R., Brown, S. E., Nashold, S. W., Knudson, D. L.,1999. Identification of duck plague virus by polymerase chain reaction. Avian Dis. 43,106-115.
[10]Yufeng Li, Hanchun Yang., et al., Molecular characterization of the genome of duck enteritis virus[J]. Virology. virol.2009,6(18):1-11.
[11]张晓根,王欣,崔世新.鹅的鸭瘟病原分离鉴定及治疗试验.中国兽医杂志2001,(37)：14-15.
[12]文明,罗永荣,袁翠霞,等.一起鹅群发生鸭瘟病例的实验室诊断[J].养禽与禽病防治.2007,(8)：40-42.
[13]陈普成,曾青华,柳金雄,等。鸭病毒性肠炎病毒UL41、UL42基因的克隆与分析。预防兽医学报中国预防兽医学报,2009,(05)：479-693.
[14]卢杏通,廖明,郭霄峰,赵明秋,刘穗,.谢淑敏,辛朝安.鸭瘟病毒基因组核酸的制备.中国兽医科技2003,(04)：49-51
[15]马相如,肖少波.VHS-蛋白一种具有mRNA剪切活性的多种功能蛋白质2005.25(2)：100-103.
[16]Herold, B. C., WuDunn, D., Soltys, N., et al.. Glycoprotein C of herpes simplex virus type 1 plays a principal role in the adsorption of virus to cells and in infectivity[J]. J Virol 1991,65(3):1090-1098.
[17]Seidel-Dugan, C., Ponce de Leon, M., Friedman, H. M., Eisenberg, R. J. and Cohen, G. H. Identification of C3b-binding regions on herpes simplex virus type 2 glycoprotein C. J Virol 1990,64(5):1897-1906.
[18]McGeoch, D. J., Dalrymple, M.A., Davison, A. J., Dolan, A., Frame, M. C., McNab, D., Perry, L. J., Scott, J. E., Taylor, P.,1988. The complete DNA sequence of the long unique region in the genome of herpes simplex virus type 1. J. Gen. Virol.69,1531-1537.
[19]Cocchi, F., Fusco, D., Menotti, L., et al.. The soluble ectodomain of herpes simplex virus gD contains a membrane-proximal pro-fusion domain and suffices to mediate virus entry[J]. Proc Natl Acad Sci USA.2004,101(19): 7445-7450.
[20]Dingwell, K. S., Johnson, D. C. The herpes simplex virus gE-gI complex facilitates cell-to-cell spread and binds to components of cell junctions [J]. J Virol.1998,72(11):8933-8942.
[21]Shawky, S. A., Sandhu, T. S. Inactivated vaccine for protection against duck virus enteritis[J]. Avian Dis.1997,41(2),:461-468.
[22]Samady L, Costigliola E, MacCormac L, et al. Deletion of the virion host shutoff protein (vhs) from Herpes simplex virus (HSV) relieves the viral block to dendritic cell activation:potential of vhs-HSV vectors for dendritic cell-Mediated immunotherapy [J]. J Virol,2003,77 (6):3768-3776.
[23]Prechtel AT, Turza NM, Kobelt D J, et al. Infection of mature dendritic cells with Herpes simplex virus type 1 dramatically reduces lymphoid chemokine-mediated migration [J]. J Gen Virol,2005,86:1645-1657.
[24]Sullivan, V., and Smith, G. L. Expression and characterization of herpes simplex virus type 1 (HSV-1) glycoprotein G (gG) by recombinant vaccinia virus: neutralization of HSV-1 infectivity with anti-gG antibody[J]. J Gen Virol. 1987,68 (10):2587-2598.
[25]Chee A V, Roizman B. Herpes simplex virusl gene products occlude the interferon signaling pathway at multiple sites [J]. J Virol,2004,78 (8): 4185-4196.
[26]Dubin, G., Jiang, H. Expression of herpes simplex virus type 1 glycoprotein L (gL) in transfected mammalian cells:evidence that gL is not independently anchored to cell membranes [J]. J Virol.1995,69(7).4564-4568.
[27]Maclean S, Belmont A S. Sequential recruitment of HAT andSWI/SNF components to condensed chromatin by VP16 [J].CurrBiol,2003,13 (3):241-6.
[28]Karr, B. M., Read, G. S. The virion host shutoff function of herpes simplex virus degrades the 5' end of a target mRNA before the 3' end[J]. Virology 1999,264(1):195-204.
[29]A.D. Kwong, J.A. Kruper, N. Frenkel, J. Virol.62,912-921(1988). 2. P. Feng, D. N. Everly Jr, G. S. Read, J. Virol.79,9651-9664 (2005).
[30]Triezenberg, S. J., Kingsbury, R. C., and McKnight, S. L. Functional dissection of VP16, the trans-activator of herpes simplex virus immediate early gene expression. Genes Dev 1988.2(6),718-729.
[31]Wysocka J, Herr W. The Herpes simplex virus VP16-inducedcomplex:the makings of a regulatory switch [J]. Biochem Sci,2003,28 (6):294-304.
[32]郭灵,谢瑶,汪华侨,等.腺病毒载体介导的1—-e-Y.基因在神经前体细胞的转染和表达[J].神经解剖学杂志,2003,19(2)：135-138.
[33]罗薇,靳艳玲,刘内生,等.鸭瘟病毒CY07株诱导的细胞凋亡及凋亡对其增殖的影响[J].中国兽医科学.2009,39(07)：575-579.
[34]Desai, P., Sexton, G. L., Huang, E., et al.. Localization of herpes simplex virus type 1 UL37 in the Golgi complex requires UL36 but not capsid structures[J]. J Virol 2008,82(22):11354-11361.
[35]李慧昕.鸭肠炎病毒部分基因结构及相关功能的初步研究.[博士学位论文].北京：中国农业科学院,2007.
[36]Sheaffer, A. K., Newcomb, W. W., Brown, J. C., et al.. Evidence for controlled incorporation of herpes simplex virus type 1 UL26 protease into capsids[J]. J Virol.2000,74(15):6838-6848.
[37]Esclatine A, Taddeo B, Evans L, et al. The Herpes simplex virus 1UL41 gene-dependent destabilization of cellular RNAs is selectiveand may be sequence-specific [J]. PNAS,2004,101(10):3603-3608.
[38]Meckes, D. G., Jr., Wills, J. W. Dynamic interactions of the UL16 tegument protein with the capsid of herpes simplex virus[J]. J Virol.2007,81(23):13028-13036.
[39]Yokota S I, Yokosawa N, Okabayashi T, et al. Induction of suppressor of cytokine signaling-3 by Herpes simplex virus type 1 contributes to inhibition of the interferon signaling pathway [J]. JVirol,2004,78 (12):6282-6286
[40]Memedula S, Belmont A S. Sequential recruitment of HAT andSWI/SNF components to.condensed chromatin by VP16 [J]. CurrBiol,2003,13 (3):241-6.
[41]Wysocka, J., Herr, W. The herpes simplex virus VP16-induced complex: the makings of a regulatory switch. Trends Biochem[J] Sci.2003,28(6):294-304.
[42]Wysocka J, Herr W. The Herpes simplex virus VP16-inducedcomplex:the makings of a regulatory switch [J]. Biochem Sci,2003,28 (6):294-304.
[43]陈淑红,韩宗玺,邵昱昊,等.鸭肠炎病毒UL6基因的分子特征[J].病毒学报.2006,22(5)：391-396.
[44]Strube, W., Auer, S., Block, W., Heinen, E., Kretzdorn, D., Rodenbach, C., and Schmeer, N. A gE deleted infectious bovine rhinotracheitis marker vaccine for use in improved bovine herpesvirus 1 control programs. Vet Microbiol 1996.53(1-2),181-189.
[45]Sullivan, V., and Smith, G. L. Expression and characterization of herpes simplex virus type 1 (HSV-1) glycoprotein G (gG) by recombinant vaccinia virus: neutralization of HSV-1 infectivity with anti-gG antibody. J Gen Virol 1987.68 (Pt 10),2587-2598.
[46]Taddeo, B. R., B. The virion host shutoff protein (UL41) of herpes simplex virus 1 is an endoribonuclease with a substrate specificity similar to that of RNase A. J Virol 2006.80(18),9341-9345
[47]Burgess, E. C., and T. M. Yuill. Vertical transmission of duck plague virus by apparently healthy DPV carrier waterfowl[J].Avian Dis.1981(25):795 800.
[48]Sp ieker J 0, Yuill TM, Burgess E C. V irulence of six strains of duck p lague virus in eigh t waterfow 1 species [J]. Journal of W ildlife D iseases. 1996,32 (3):453-460.
[49]Tigges M A, Leng S, Johnson D C, et al. Human herpes simplex virus (HSV)-specific CD8+ CTL clones recognize HSV-2-infected fibroblasts after treatment with IFN-gamma or when virion host shutoff functions are disabled [J]. Immunol,1996,156:3901-3910.
[50]程安春,汪铭书等,现代禽病诊断和防治全书[M],成都,四川大学出版社,1997,32：505。
[51]尚世荣.鹅感染鸭瘟病毒的诊治报告 [J].畜牧兽医科技信息.2007,(12)：102-103.
[52]吕桂霞,刁有祥.“新型鸭瘟”流行病学及病原特性的研究.2003,山东农大学位论文.
[53]陆新浩,刘鸿,朱梦代等,一种新疫病在浙江的流行及防治初探[C],中国畜牧兽医学会禽病学分会第十一次学术研讨会论文集,2002 396 397
[54]童光志,王云峰,等.基因工程疫苗原理与方法[M].北京.2009,化学工业出版社.
[55]A.D. Kwong, N. Frenkel, Proc. Natl. Acad. Sci. USA 84,1926-1930 (1987).
[56]A. A. Oroskar, G. S. Read, J. Virol.61,604-606 (1987).
[57]T. L. Keadle, L. A. Morrison, J. L. Morris, J. S. Pepose, P.M. Stuart, J. Virol.76,3615-3625 (2002).
[58]T. J. Smith, L.A. Morrison, D. A. Leib, J. Virol.76,2054-2061(2002).
[59]R. L. Witter, K.A. Schat, in Diseases of Poultry, ed. by Y. M. Saif, H. J. Barnes, J. R. Glisson, A.M. Fadly, L. R. McDougald, D. E. Swayne (Iowa State Press, Ames,2003), pp.407-465.
[60]R.L. Witter, Avian Dis.41,149-163 (1997).
[61]L. D. Bacon, H. D. Hunt, H. H. Cheng, Poult. Sci.79,1082-1093(2000).
[62]乔代蓉.限制性内切酶分析鸭瘟病毒DNA.四川农业大学学报1992.10(001),172-177.
[63]李玉峰,何叶峰,黄兵.鸭瘟病毒基因组的提取和限制性酶切分析.中国家禽2007.(05).12-14.
[64]李子剑,李玉峰,陈苏,宋敏训,李云龙.鸭瘟病毒基因组核酸的提取方法比较.山东师范大学学报(自然科学版)2008.(01).134-136.
[65]J.萨姆布鲁克等著,黄培堂等译.分子克隆实验操作指南(第三版).北京：科学出版社.2002.
[66]Perez-Parada J, Saffran H A, Smiley J R. RNA Degradation induced by the Herpes simplex virus vhs protein proceeds 5' to 3' in vitro [J]. J Virol,2004, 78 (23):13391-13394.
[67]Q. Lam, C. A. Smibert, K. E. Koop, C. Lavery, J. P. Capone, S. P. Weinheimer, J. R. Smiley, EMBO J.15,2575-2581 (1996).
[68]B. Taddeo, M. T. Sciortino, W. Zhang, B. Roizman, Proc. Natl.Acad. Sci. USA 104,12163-12168 (2007).
[69]D. Koppers-Lalic, F. A. Rijsewijk, S. B. Verschuren, J.A. vanGaans-Van den Brink, A. Neisig, M. E. Ressing, J. Neefjes, E. J. Wiertz, J. Gen. Virol. 82,2071-2081 (2001).
[70]R. S. Gopinath, A. P. Ambagala, S. Hinkley, S. Srikumaran, ViralImmunol. 15,595-608 (2002).
[71]Jerome, K. R., Chen, Z., Lang, R., et al.. HSV and glycoprotein J inhibit caspase activation and apoptosis induced by granzyme B or Fas[J]. J Immunol. 2001,167(7):3928-3935.
[72]Baines, J. D., Wills, E., Jacob, R. J., et al.. Glycoprotein M of herpes simplex virus 1 is incorporated into virions during budding at the inner nuclear membrane[J]. J Virol.2007,81(2):800-812.
[73]ato C, Ohmiya R, Mizuno T. A rap id method for disrup tinggenes in the Escherichia coli genome. Biosci BiotechnolBiochem,1998,62:1826～1829
[74]Sheaffer, A. K., Newcomb, W. W., Brown, J. C., Gao, M., Weller, S. K., and Tenney, D. J. Evidence for controlled incorporation of herpes simplex virus type 1 UL26 protease into capsids. J Virol 2000.74(15),6838-6848.
[75]Smiley, J. R. Herpes simplex virus virion host shutoff protein:immune evasion mediated by a viral RNase? J Virol 2004.78(3),1063-1068.
[76]Sonoda, K., Sakaguchi, M., Okamura, H., Yokogawa, K., Tokunaga, E., Tokiyoshi, S., Kawaguchi, Y., and Hirai, K. Development of an effective polyvalent vaccine against both Marek's and Newcastle diseases based on recombinant Marek's disease virus type 1 in commercial chickens with maternal antibodies. J Virol 2000.74(7),3217-3226.
[77]Roop, C., Hutchinson, L., and Johnson, D. C. A mutant herpes simplex virus type 1 unable to express glycoprotein L cannot enter cells, and its particles lack glycoprotein H. J Virol 1993.67(4),2285-2297.
[78]Sakaguchi, M., Nakamura, H., Sonoda, K., Okamura, H., Yokogawa, K., .Matsuo, K., and Hira, K. Protection of chickens with or without maternal antibodies against both Marek's and Newcastle diseases by one-time vaccination with recombinant vaccine of Marek's disease virus type 1. Vaccine 1998.16(5), 472-479.
[79]Thomsen, D. R., Newcomb, W. W., Brown, J. C., and Homa, F. L. Assembly of the herpes simplex virus capsid:requirement for the carboxyl-terminal twenty-five amino acids of the proteins encoded by the UL26 and UL26.5 genes. J Virol 1995.69(6),3690-3703.
[80]Shawky, S. and Schat, K. A. Latency sites and reactivation of duck enteritis virus. Avian Dis 2002.46(2),308-313.
[81]Poteete A R, Fenton A C. Genetic requirements of phagelambda red2mediated gene rep lacement in Escherichia coli K212. J Bacteriol,2000,182:2336～ 2340
[82]Kuzminov A. Recombinational repair of DNA damage inEscherichia coli and bacteriophage lambda. MicrobiolMol BiolRev,1999,63:751～813
[83]Kovall R, Matthews B W. Toroidal structure of lambdaexonuclease. Science, 1997,277:1824-1827
[84]Kowalczykowski S C, Dixon D A, Rehrauer W M, et al.Biochemistry of homologous recombination in Escherichia coli. Microbiol Rev,1994,58:401～ 465
[85]Roca A I, CoxM M. RecA p rotein:structure, function, androle in recombinationalDNA repair. ProgNucleic Acid ResMolBiol,1997,56:129～223
[86]Kogoma T, Cadwell G W, Barnard K G, et al. The DNArep lication p riming p rotein, PriA, is required for homologousrecombination and double2strand break repair. J Bacteriol,1996,178:1258～1264
[87]Dykstra C C, Prasher D, Kushner S R. Physical andbiochemical analysis of the cloned recB and recC genes ofEscherichia coli K212. J Bacteriol,1984, 157:21-27
[88]Murphy K C. Use of bacteriophage 1 recombination functions top romote gene rep lacement in Escherichia coli. J. Bacteriol,1998,180:2063～2071
[89]Murphy K C, Campellone K G, Poteete A R. PCR2mediated gene rep lacement in Escherichia coli. Gene,2000,246:321～330
[90]Datsenko K A, Wanner B L. One2step inactivation of chromosomal genes in Escherichia coli K212 using PCR p roducts. Proc Natl Acad Sci USA,2000, 97: 6640-6645
[91]侯松旺,陈新文,王汉中,等1一种以PCR产物直接构建同源重组杆状病毒的方法1中国科学(C辑),2003,33(2)：169-174
[92]王恒梁,冯尔玲,史兆兴,等1Red系统快速敲除痢疾杆菌asd基因1军事医学科学院刊,2002,26(3)：161-164
[93]张全,高会杰,佟明友.Red重组技术研究进展.中国生物工程杂志.2006,26(1)：81-86.
[94]Strand S S, Leib D A. Role of the VP16-Binding domain of VHS inviral growth, host shutoff activity, and pathogenesis [J]. J Virol,2004,78 (24): 13562-13572.
[95]Feng P, Everly D N, Read G S. mRNA Decay during Herpes simplexvirus (HSV) infections:protein-protein interactions involving theHSV virion host shutoff protein and translation factors eIF4H andeIF4A [J]. Virology,2005,79(15): 9651-9664.
[96]李子剑.鸭肠炎病毒基因组复制非必需区的筛选.[硕士学位论文].济南：山 东师范大学,2008
[97]Geiss B J, et al. J Virol,2000,74:11137-11144.
[98]Hinkley S, Ambagala A P N, Jones C J, et al. A vhs-like activity of Bovine herpesvirus-1 [J]. Arch Virol,2000,145:2027-2046.