带器妊娠妇女血清，组织铜的相关研究
摘要
目的:通过测定含铜宫内节育器(Cu-IUD)妊娠妇女的子宫蜕膜、胚胎绒毛及血清铜离子的含量,与正常妊娠者相对比,为探讨带器妊娠妇女组织铜离子的含量对子代胚胎的安全性的研究提供参考。同时分析带Cu-IUD妊娠妇女的相关特征,探讨带Cu-IUD失败的原因。
方法:采用随机对照临床研究,选取25例带Cu-IUD妊娠者作为实验组,同时选取25例同孕周正常妊娠者作为对照组,分别测定实验组和对照组的子宫蜕膜、胚胎绒毛及血清铜离子的含量。
结果:测定带Cu-IUD妊娠组妇女的血清铜离子其均值为0.79±0.15mg/L,子宫蜕膜、胚胎绒毛中铜离子含量均值分别为0.91±0.51mg/kg,0.72±0.50 mg/kg;正常妊娠组妇女的血清铜离子其均值为0.71±0.15mg/L,子宫蜕膜、胚胎绒毛中铜离子含量均值分别为0.57±0.21mg/kg,0.46±0.21 mg/kg;比较实验组和对照组妇女的血清铜离子的含量,实验组与对照组相比,无明显差异,P＞0.05,子宫蜕膜中铜离子含量有明显差异,P＜0.05,胚胎绒毛中铜离子含量有明显差异,P＜0.05。同时分别对实验组血清铜离子与绒毛中铜离子含量、血清铜离子与蜕膜中铜离子含量、对照组血清铜离子与绒毛中铜离子含量、血清铜离子与蜕膜中铜离子含量进行了相关性分析,结果表明皆无统计学意义,P＞0.05。带Cu-IUD妊娠妇女导致带器失败的相关因素中,与使用者本身的年龄、孕产次、放置时间等因素都有相关性,而与子宫位置无关。
结论:Cu-IUD中的铜离子只是释放在宫腔局部产生作用,对于全身铜代谢没有影响。而局部过量的铜离子产生的细胞和生殖毒性作用对子代的影响有待于进一步研究。
Objective:Through determine the copper content of uterus deciduas,embryo villus and blood serum,comparing with the content of the normal pregnancy women intended to terminate pregnancy,we investigate the tissue copper content if had an toxicity influence on offspring.Meanwhile analyze the characteristics of pregnancy women in Cu-IUD intended to terminate pregnancy,we explode the causes of Cu-IUD failure.
Methods:With the method of random-controll clinical research,twenty-five pregnancy women in Cu-IUD intended to terminate pregnancy are experimental group,twenty-five normal pregnancy women intended to terminate pregnancy are control group.To determine the copper content of uterus deciduas,embryo villus and blood serum in experimental group and control group.And we take the careful analysis of characteristics of pregnancy women in Cu-IUD intended to terminate pregnancy,we explode the causes of Cu-IUD failure.
Results:The copper content of uterus decidua,embryo villus and blood serum in experimental group respectively are 0.91±0.51mg/kg,0.72±0.50mg/kg,0.79±0.15 mg/L;the content of control group in uterus deciduas,embryo villus and blood serum are 0.57±0.21 mg/kg,0.46±0.21 mg/kg,0.71±0.15mg/L;To compare the copper content disparities in uterus deciduas and embryo villus between experimental group and control group,there are obvious differences.But the serum copper content has no difference between the two groups.At the same time,take the correlation analysis about the Cu content between serum and embryo villus,serum and uterus deciduas of control group,so is experimental group,the results have no difference,all of them have no relation.Of the related factors in Cu-IUD faiure,age、parity、the time of placement have correlation;whereas the position of the uterus has no correlation. Furthermore,the level of placement,the quality of follow-up also have the relationship with the IUD placement.
Conclusion:Cu ion released from Cu-IUD only had an influence on the uterus,having no difference on the Cu metabolism all over the body.The local rising Cu content produced cell and reproduction toxicity,which will be researched further.
方法:采用随机对照临床研究,选取25例带Cu-IUD妊娠者作为实验组,同时选取25例同孕周正常妊娠者作为对照组,分别测定实验组和对照组的子宫蜕膜、胚胎绒毛及血清铜离子的含量。
结果:测定带Cu-IUD妊娠组妇女的血清铜离子其均值为0.79±0.15mg/L,子宫蜕膜、胚胎绒毛中铜离子含量均值分别为0.91±0.51mg/kg,0.72±0.50 mg/kg;正常妊娠组妇女的血清铜离子其均值为0.71±0.15mg/L,子宫蜕膜、胚胎绒毛中铜离子含量均值分别为0.57±0.21mg/kg,0.46±0.21 mg/kg;比较实验组和对照组妇女的血清铜离子的含量,实验组与对照组相比,无明显差异,P＞0.05,子宫蜕膜中铜离子含量有明显差异,P＜0.05,胚胎绒毛中铜离子含量有明显差异,P＜0.05。同时分别对实验组血清铜离子与绒毛中铜离子含量、血清铜离子与蜕膜中铜离子含量、对照组血清铜离子与绒毛中铜离子含量、血清铜离子与蜕膜中铜离子含量进行了相关性分析,结果表明皆无统计学意义,P＞0.05。带Cu-IUD妊娠妇女导致带器失败的相关因素中,与使用者本身的年龄、孕产次、放置时间等因素都有相关性,而与子宫位置无关。
结论:Cu-IUD中的铜离子只是释放在宫腔局部产生作用,对于全身铜代谢没有影响。而局部过量的铜离子产生的细胞和生殖毒性作用对子代的影响有待于进一步研究。
Objective:Through determine the copper content of uterus deciduas,embryo villus and blood serum,comparing with the content of the normal pregnancy women intended to terminate pregnancy,we investigate the tissue copper content if had an toxicity influence on offspring.Meanwhile analyze the characteristics of pregnancy women in Cu-IUD intended to terminate pregnancy,we explode the causes of Cu-IUD failure.
Methods:With the method of random-controll clinical research,twenty-five pregnancy women in Cu-IUD intended to terminate pregnancy are experimental group,twenty-five normal pregnancy women intended to terminate pregnancy are control group.To determine the copper content of uterus deciduas,embryo villus and blood serum in experimental group and control group.And we take the careful analysis of characteristics of pregnancy women in Cu-IUD intended to terminate pregnancy,we explode the causes of Cu-IUD failure.
Results:The copper content of uterus decidua,embryo villus and blood serum in experimental group respectively are 0.91±0.51mg/kg,0.72±0.50mg/kg,0.79±0.15 mg/L;the content of control group in uterus deciduas,embryo villus and blood serum are 0.57±0.21 mg/kg,0.46±0.21 mg/kg,0.71±0.15mg/L;To compare the copper content disparities in uterus deciduas and embryo villus between experimental group and control group,there are obvious differences.But the serum copper content has no difference between the two groups.At the same time,take the correlation analysis about the Cu content between serum and embryo villus,serum and uterus deciduas of control group,so is experimental group,the results have no difference,all of them have no relation.Of the related factors in Cu-IUD faiure,age、parity、the time of placement have correlation;whereas the position of the uterus has no correlation. Furthermore,the level of placement,the quality of follow-up also have the relationship with the IUD placement.
Conclusion:Cu ion released from Cu-IUD only had an influence on the uterus,having no difference on the Cu metabolism all over the body.The local rising Cu content produced cell and reproduction toxicity,which will be researched further.
引文
[1]Johansson ED.Future developments in hormonal contraception[J].Am J Obstet Gynecol,2004,190:69-71.
[2]Richter.R.Ein Mictel zur Verhutung der konzeption(A means of preventing pregnancy).Med.Wochenschr,1909,35:1525-1527.
[3]Zipper Z A,Tatum H J,Pastene L,et al.Metaltic copper as an intrauterine contraceptive adjunct to the“T”device[J].American Journal of Obstetrics and Gynecology,1969,105:1274-1278.
[4]Juhani Toivonen,Tapani Luukkainen.Randomized comparison of levonorgestrel-and copper-releasing intrauterine systems immediately after abortion,with five years'follow-up[J].Contraception,2003,68:31-34.
[5]Xiaoming Cao,Wenhao Zhang,Xun Zhao,et al.Three-year efficacy and acceptability of the GyneFix200 intrauterine system[J].Contraception,2004,69:207-211.
[6]Ahmed E-Tagy,Ezzat Sakr,David C.Sokal,et al.Safety and acceptability of post-abortal IUD insertion and the importance of counseling[J].Contraception,2003,107,67:229-234.
[7]Olav Meirik,Timothy M.M.Farley,Irving Sivin.Safety and Efficacy of Levonorgestrel Implant,Intrauterine Device,and Sterilization[J].Obstetrics and Gynecology,2001,97(4):539-547.
[8]董白桦,侯桂华,张萍等。长期放置宫内节育器的安全性研究[J]。山东大学学报(医学版),2002,40(5):445-447。
[9]Roberto Rivera,Kim Best.Current opinion:Consensus statement on intrauterine contraception[J].Contraception,2002,65:385-388.
[10]David A Grimes.Intrauterine device and upper-genital-tract infection[J].Lancet,2000,356:1013-1019.
[11]J.B.Otero-Flores,F.J.Guerrero-Carre?o,L.A.V(?)zquez-Estrada.A comparative randomized study of three different IUDs in nulliparous Mexican women[J].Contraception,2003,67:273-276.
[12]J.B.Dubuisson,The Mirena Study Group,E.Mugnier.Acceptability of the levonorgestrel-releasing intrauterine system after discontinuation of previous contraception:results of a French clinical study in women aged 35 to 45years[J].Contraception,2002,66:121-128.
[13]Ver(?)nica Schiappacasse,Soledad Diaz,Ana Zepeda,et al.Health and growth of infants breastfed by Norplant contraceptive implants users:a six-year follow-up study[J].Contraception,2002,66:57-65.
[14]Chiun-Fang Chiou,James Trussell,Eileen Reyes,at al.Economic analysis of contraceptives for women[J].Contraception,2003,68:3-10.
[15]邹燕,雷贞武。宫内节育器的流行病学研究近况[J]。实用妇产科杂志,2003,19(6):321-323。
[16]Harris ED.Basic and clinical aspects of copper.Cril Rev Clin Lab Sci,2003,40:547-586.
[17]滕凯,邱国风,俞斌武等。妊娠期血清微量元素的变化[J]。中国优生与遗传杂志,2007,15(9):32-33。
[18]李红霞,张娜,张政。妊娠妇女常量和必需微量元素的含量及分析[J]。中国妇产科临床杂志,2006,7(6):433-634。
[19]秦岩,陈魁敏,胡桂娟等。血清中铜、铁、锌、钙变化对妊娠各期的影响[J]。中国妇幼健康研究,2007,18(2):107-108。
[20]Wang H et al.Prostaglandins Other Lipid Mediat,2005,77:84.
[21]张曦倩,庞战军,陈士岭等。蜕膜细胞条件培养液对滋养细胞侵袭调节基因表达的影响[J]。第一军医大学学报,2002,22(7):588-591。
[22]Bisehof P,Meisser A,CamPana A.Paraerine and autocrine regulators of Trophoblast invasion-a review.Plaeenta.2000,21:555-560.
[23]Aplin J D,Laeey H,Haigh T,et al.Growth factor-extracellular matrix Synergy in the control of trophoblast invasion.Bioehem Soc Trans.2000,28(2):199-202.
[24]Mangia A,Tommasi S,Reshkin SJ,et al.Gonadotropin releasing hormon reeept or in primary breast cancer:comparison of immunohist oehemieal,radioligand and Western blot analyses[J].Oncol ReP,2002,9(5):1127-1132.
[25]蔡文娟,董彩金,陈拽生等。早孕绒毛组织中铜的测定[J]。河北医学院学报,1991,12(2):21-22。
[26]Friberg L,Nordberg GF,Kessler E,et al.Handbook of the Toxicology of Metals[M].Amsterdam:Elsevier Science Publishers B.V,1986.2:247.
[27]Barlow SM,Kniqht AF.Intrauterine exposure to copper IUDs and prenatal development in the rat.J Reprod Fertil.,1981,62(1):123-30.
[28]张川川等。Cu220C与TCu380A IUD体外细胞毒性研究[J]。中国计划生育学杂志,2008,7(153):410-412。
[29]Wang D,Altmann DR.Socio—demographic determinants of intrauterine device use and failure in China[J].Hum Reprod,2002,17(5):1226-1232.
[30]Avecilla—Palau A,Moreno V.Uterine factors and risk of pregnancy in IUD users.,a nestedease—control study[J].Contraception,2003,67:235-239.
[31]杨秀兰,庄留琪等。更新换代后的活性IUD安全性和有效性随访研究[J]。生殖与避孕,2001,21(4):216-220。
[32]Ramirez Hidalgo A,Pujol Ribera E.Use of the intrauterine device:efficacy and safety[J].EurJ Contracept Reprod Health Care.2000,5(3):198-207.
[33]速存梅。宫内节育器避孕失败的影响因素[J]。中国计划生育杂志,2005,(10):637-638。
[34]冯睿,钱玲惠,吴永红等。宫内节育器放置时间的研究[J]。中国实用妇科与产科杂志,2002,18(8):488。
[35]Gocmen A,Demirpolat N,Avsin H.The efficacy of immediate post—a borlion intrauterine device insertion[J].Clin Exp Obstet Gynecol,2002,29(4),274-276.
[36]Moussa A.Evaluation of postabortion 1UD insertion in Egyptian women[J].Contraception,2001,63(6):315-317.
[37]El-tagy A,Sakr E,Sokal DC,lssa AH.Safety and acceptability of post-abortal IUD insertion and the importance of counseling[J].Contraception,2003,67(3):229-234.
[38]王厚勤。带铜T型宫内节育器带器妊娠205例分析[J]。中国误诊学杂志,2002,2(8):1216-1217。
[39]杨强,陆铭策。1245例金属避孕环失败原因的初步分析[J]。生殖与避孕,1988,3:73。
[40]赵玉风。带器妊娠148例临床分析[J]。中国计划生育学杂志,2003,8(11):692。
[41]Rivera R,Chen-Mok M,McMullen S.Analysis of client characteristics that may affect early discontinuation of the TCu380A IUD[J].Contraception,1999,60:155-160.
[42]谢黎,罗世俊,刘莉。产褥未期放置MLCu375SI和TCu380A的比较性研究[J]。实用妇产科杂志,2000,16(6):298-299。
[43]汪蓉芬,董光华,华小梅等。四种宫内节育器的对比研究[J]。中国计划生育学杂志,1996,4(1):21-24。
[44]Farr G,Amatya R,Doh A,et al.An evaluation of the copper-380A IUD safety and efficacy at three African centers[J].Contraception,1996,53:293-298.
[45]Harrison—woolyrch M,Ashton J,Coulter D.Uterine Perforation on intrauterine device insertion:is the incidence higher than previously reported?Contraception,2003,67(1):53-56.
[46]Farr G,Rivera R,Amatva R.Non-physician insertion of IUDs:clinical outcomes among TCu380A insertion in three developing country clinics[J].Adv Contracept,1998,14(1):45-47.
[1]Harris ED.Basic and clinical aspects of copper[J].Cril Rev Clin Lab Sci,2003,40:547-586.
[2]Waphir RA.Copper absorption and bioavailablity[J].Am J Clin Nutr,1998,67:1054-1060.
[3]Hellman NE,Kono S,Mancini GM,et al.Mechanism of copper incorporation into human ceruloplasmin[J].J Biol Chem,2002,277:46632-46638.
[4]Terada K,Aiba N,Yang XL,et al.Biliary excretion of copper in LEC rat after introduction of copper transporting P-type ATPase,ATP7B.FEBS Lett,1999,448:53-56.
[5]Fatemi N,Sarkar B.Structural and functional insights of Wilson disease copper-transporting ATPase[J].J Bioenerg Biomember,2002,34:339-349.
[6]Yamaguchi Y,Heiny ME,Gitlin JD.Isolation and characterization of a human liver cDNA as a candidate gene for Wilson disease[J].Biochem Biophys Res Commun,1998,197:271-277.
[7]Calabrease L.Structure to function relationshios in ceruloplasmin:a “moonlighting”protein.Cell Mol Life Sci,2002,59;1413-1427.
[8]王世英。微量元素铜在妇产科的临床意义[J]。国外医学—妇产科学分册,1988,1-3。
[9]Larsen B,et al.Actions of placental and fetal adrend steroid hormones in primate pregnancy[J].Eur J Cancer 1980,16:415.
[10]王夔.。生命科学中的微量元素(上卷)[M].。北京:中国计量出版社,1991,14(64),219-225。
[11]Ambidg eM,Hackshaw A,Wald N.Neural tube defects and serum zinc[J].Br Obstet Gynecol,1993,100:746.
[12]刘希兰,陈陵。微量元素与胚胎生长发育[J]。微量元素与健康研究,,2003,20:52-54。
[13]郭晓莉.。自然流产孕妇体内微量元素含量的变化[J]。广东微量元素科学,2000,7:29-31。
[14]张惠丹等。铜及赖氨酰氧化酶与胎膜早破的相关性研究[J]。中华妇产科学,2006,1(14):7-11。
[15]万长春,汪泓,商学军等。沙眼衣原体感染与生精细胞凋亡。现代检验医学杂志,2003,18(1):12-13。
[16]杨爱景。解脲支原体沙眼衣原体感染与男性不育关系的探讨。中国艾滋病性病,2006,12(1):47-48。
[17]Guleria K,Aqarwal N,Mishra K,et al.Evaluation of endometrial steroid receptors and cell mitotic activity in women using copper intrauterine device:Can Cu-T prevent endometrial cancer?J Obstet Gynaecol Res.2004 Jun;30(3):181-7.
[18]张川川等。TCu220C与TCu380A IUD体外细胞毒性研究[J]。中国计划生育学杂志,2008,7(153):410-412。
[19]Barlow SM,Kniqht AF,et al.Intrauterine exposure to copper IUDs and prenatal develooment in the rat.J Reprod Fertil.,1981,62(1):123-30.
[20]胡纪源,吕达平,王共强等。肝豆状核变性的临床误诊研究[J]。中华医学杂志,2001;81:642。
[2]Richter.R.Ein Mictel zur Verhutung der konzeption(A means of preventing pregnancy).Med.Wochenschr,1909,35:1525-1527.
[3]Zipper Z A,Tatum H J,Pastene L,et al.Metaltic copper as an intrauterine contraceptive adjunct to the“T”device[J].American Journal of Obstetrics and Gynecology,1969,105:1274-1278.
[4]Juhani Toivonen,Tapani Luukkainen.Randomized comparison of levonorgestrel-and copper-releasing intrauterine systems immediately after abortion,with five years'follow-up[J].Contraception,2003,68:31-34.
[5]Xiaoming Cao,Wenhao Zhang,Xun Zhao,et al.Three-year efficacy and acceptability of the GyneFix200 intrauterine system[J].Contraception,2004,69:207-211.
[6]Ahmed E-Tagy,Ezzat Sakr,David C.Sokal,et al.Safety and acceptability of post-abortal IUD insertion and the importance of counseling[J].Contraception,2003,107,67:229-234.
[7]Olav Meirik,Timothy M.M.Farley,Irving Sivin.Safety and Efficacy of Levonorgestrel Implant,Intrauterine Device,and Sterilization[J].Obstetrics and Gynecology,2001,97(4):539-547.
[8]董白桦,侯桂华,张萍等。长期放置宫内节育器的安全性研究[J]。山东大学学报(医学版),2002,40(5):445-447。
[9]Roberto Rivera,Kim Best.Current opinion:Consensus statement on intrauterine contraception[J].Contraception,2002,65:385-388.
[10]David A Grimes.Intrauterine device and upper-genital-tract infection[J].Lancet,2000,356:1013-1019.
[11]J.B.Otero-Flores,F.J.Guerrero-Carre?o,L.A.V(?)zquez-Estrada.A comparative randomized study of three different IUDs in nulliparous Mexican women[J].Contraception,2003,67:273-276.
[12]J.B.Dubuisson,The Mirena Study Group,E.Mugnier.Acceptability of the levonorgestrel-releasing intrauterine system after discontinuation of previous contraception:results of a French clinical study in women aged 35 to 45years[J].Contraception,2002,66:121-128.
[13]Ver(?)nica Schiappacasse,Soledad Diaz,Ana Zepeda,et al.Health and growth of infants breastfed by Norplant contraceptive implants users:a six-year follow-up study[J].Contraception,2002,66:57-65.
[14]Chiun-Fang Chiou,James Trussell,Eileen Reyes,at al.Economic analysis of contraceptives for women[J].Contraception,2003,68:3-10.
[15]邹燕,雷贞武。宫内节育器的流行病学研究近况[J]。实用妇产科杂志,2003,19(6):321-323。
[16]Harris ED.Basic and clinical aspects of copper.Cril Rev Clin Lab Sci,2003,40:547-586.
[17]滕凯,邱国风,俞斌武等。妊娠期血清微量元素的变化[J]。中国优生与遗传杂志,2007,15(9):32-33。
[18]李红霞,张娜,张政。妊娠妇女常量和必需微量元素的含量及分析[J]。中国妇产科临床杂志,2006,7(6):433-634。
[19]秦岩,陈魁敏,胡桂娟等。血清中铜、铁、锌、钙变化对妊娠各期的影响[J]。中国妇幼健康研究,2007,18(2):107-108。
[20]Wang H et al.Prostaglandins Other Lipid Mediat,2005,77:84.
[21]张曦倩,庞战军,陈士岭等。蜕膜细胞条件培养液对滋养细胞侵袭调节基因表达的影响[J]。第一军医大学学报,2002,22(7):588-591。
[22]Bisehof P,Meisser A,CamPana A.Paraerine and autocrine regulators of Trophoblast invasion-a review.Plaeenta.2000,21:555-560.
[23]Aplin J D,Laeey H,Haigh T,et al.Growth factor-extracellular matrix Synergy in the control of trophoblast invasion.Bioehem Soc Trans.2000,28(2):199-202.
[24]Mangia A,Tommasi S,Reshkin SJ,et al.Gonadotropin releasing hormon reeept or in primary breast cancer:comparison of immunohist oehemieal,radioligand and Western blot analyses[J].Oncol ReP,2002,9(5):1127-1132.
[25]蔡文娟,董彩金,陈拽生等。早孕绒毛组织中铜的测定[J]。河北医学院学报,1991,12(2):21-22。
[26]Friberg L,Nordberg GF,Kessler E,et al.Handbook of the Toxicology of Metals[M].Amsterdam:Elsevier Science Publishers B.V,1986.2:247.
[27]Barlow SM,Kniqht AF.Intrauterine exposure to copper IUDs and prenatal development in the rat.J Reprod Fertil.,1981,62(1):123-30.
[28]张川川等。Cu220C与TCu380A IUD体外细胞毒性研究[J]。中国计划生育学杂志,2008,7(153):410-412。
[29]Wang D,Altmann DR.Socio—demographic determinants of intrauterine device use and failure in China[J].Hum Reprod,2002,17(5):1226-1232.
[30]Avecilla—Palau A,Moreno V.Uterine factors and risk of pregnancy in IUD users.,a nestedease—control study[J].Contraception,2003,67:235-239.
[31]杨秀兰,庄留琪等。更新换代后的活性IUD安全性和有效性随访研究[J]。生殖与避孕,2001,21(4):216-220。
[32]Ramirez Hidalgo A,Pujol Ribera E.Use of the intrauterine device:efficacy and safety[J].EurJ Contracept Reprod Health Care.2000,5(3):198-207.
[33]速存梅。宫内节育器避孕失败的影响因素[J]。中国计划生育杂志,2005,(10):637-638。
[34]冯睿,钱玲惠,吴永红等。宫内节育器放置时间的研究[J]。中国实用妇科与产科杂志,2002,18(8):488。
[35]Gocmen A,Demirpolat N,Avsin H.The efficacy of immediate post—a borlion intrauterine device insertion[J].Clin Exp Obstet Gynecol,2002,29(4),274-276.
[36]Moussa A.Evaluation of postabortion 1UD insertion in Egyptian women[J].Contraception,2001,63(6):315-317.
[37]El-tagy A,Sakr E,Sokal DC,lssa AH.Safety and acceptability of post-abortal IUD insertion and the importance of counseling[J].Contraception,2003,67(3):229-234.
[38]王厚勤。带铜T型宫内节育器带器妊娠205例分析[J]。中国误诊学杂志,2002,2(8):1216-1217。
[39]杨强,陆铭策。1245例金属避孕环失败原因的初步分析[J]。生殖与避孕,1988,3:73。
[40]赵玉风。带器妊娠148例临床分析[J]。中国计划生育学杂志,2003,8(11):692。
[41]Rivera R,Chen-Mok M,McMullen S.Analysis of client characteristics that may affect early discontinuation of the TCu380A IUD[J].Contraception,1999,60:155-160.
[42]谢黎,罗世俊,刘莉。产褥未期放置MLCu375SI和TCu380A的比较性研究[J]。实用妇产科杂志,2000,16(6):298-299。
[43]汪蓉芬,董光华,华小梅等。四种宫内节育器的对比研究[J]。中国计划生育学杂志,1996,4(1):21-24。
[44]Farr G,Amatya R,Doh A,et al.An evaluation of the copper-380A IUD safety and efficacy at three African centers[J].Contraception,1996,53:293-298.
[45]Harrison—woolyrch M,Ashton J,Coulter D.Uterine Perforation on intrauterine device insertion:is the incidence higher than previously reported?Contraception,2003,67(1):53-56.
[46]Farr G,Rivera R,Amatva R.Non-physician insertion of IUDs:clinical outcomes among TCu380A insertion in three developing country clinics[J].Adv Contracept,1998,14(1):45-47.
[1]Harris ED.Basic and clinical aspects of copper[J].Cril Rev Clin Lab Sci,2003,40:547-586.
[2]Waphir RA.Copper absorption and bioavailablity[J].Am J Clin Nutr,1998,67:1054-1060.
[3]Hellman NE,Kono S,Mancini GM,et al.Mechanism of copper incorporation into human ceruloplasmin[J].J Biol Chem,2002,277:46632-46638.
[4]Terada K,Aiba N,Yang XL,et al.Biliary excretion of copper in LEC rat after introduction of copper transporting P-type ATPase,ATP7B.FEBS Lett,1999,448:53-56.
[5]Fatemi N,Sarkar B.Structural and functional insights of Wilson disease copper-transporting ATPase[J].J Bioenerg Biomember,2002,34:339-349.
[6]Yamaguchi Y,Heiny ME,Gitlin JD.Isolation and characterization of a human liver cDNA as a candidate gene for Wilson disease[J].Biochem Biophys Res Commun,1998,197:271-277.
[7]Calabrease L.Structure to function relationshios in ceruloplasmin:a “moonlighting”protein.Cell Mol Life Sci,2002,59;1413-1427.
[8]王世英。微量元素铜在妇产科的临床意义[J]。国外医学—妇产科学分册,1988,1-3。
[9]Larsen B,et al.Actions of placental and fetal adrend steroid hormones in primate pregnancy[J].Eur J Cancer 1980,16:415.
[10]王夔.。生命科学中的微量元素(上卷)[M].。北京:中国计量出版社,1991,14(64),219-225。
[11]Ambidg eM,Hackshaw A,Wald N.Neural tube defects and serum zinc[J].Br Obstet Gynecol,1993,100:746.
[12]刘希兰,陈陵。微量元素与胚胎生长发育[J]。微量元素与健康研究,,2003,20:52-54。
[13]郭晓莉.。自然流产孕妇体内微量元素含量的变化[J]。广东微量元素科学,2000,7:29-31。
[14]张惠丹等。铜及赖氨酰氧化酶与胎膜早破的相关性研究[J]。中华妇产科学,2006,1(14):7-11。
[15]万长春,汪泓,商学军等。沙眼衣原体感染与生精细胞凋亡。现代检验医学杂志,2003,18(1):12-13。
[16]杨爱景。解脲支原体沙眼衣原体感染与男性不育关系的探讨。中国艾滋病性病,2006,12(1):47-48。
[17]Guleria K,Aqarwal N,Mishra K,et al.Evaluation of endometrial steroid receptors and cell mitotic activity in women using copper intrauterine device:Can Cu-T prevent endometrial cancer?J Obstet Gynaecol Res.2004 Jun;30(3):181-7.
[18]张川川等。TCu220C与TCu380A IUD体外细胞毒性研究[J]。中国计划生育学杂志,2008,7(153):410-412。
[19]Barlow SM,Kniqht AF,et al.Intrauterine exposure to copper IUDs and prenatal develooment in the rat.J Reprod Fertil.,1981,62(1):123-30.
[20]胡纪源,吕达平,王共强等。肝豆状核变性的临床误诊研究[J]。中华医学杂志,2001;81:642。
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