人工感染放线杆菌（Ⅰ型）对猪肺脏和肺门淋巴结的损伤机理研究
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摘要
目的：
PP在高密度饲养的现代大型规模化养猪场所造成的损失及危害相当严重。PP的病原学、流行特点及检测方法等一直是国内外研究人员和学者关注的焦点；关于APP感染对猪机体组织损伤及免疫相关的系统研究较为少见。本研究旨在：(1)建立PP人工感染疾病模型；(2)监测感染APP后,猪的病症、血液常规及生化指标、抗氧化功能、免疫状况(红细胞免疫、T细胞免疫与体液免疫及细胞因子水平)及病理变化；(3)利用含43,603个探针的Agilent猪类全基因组芯片检测感染APP猪的肺和肺门淋巴结基因表达谱变化,探索感染组织损伤的分子机制。
结果：
1.采用鼻腔气雾法,按猪每千克体重鼻腔喷雾含APP3.5-4×107CFU/ml的APP(I型)稀释液0.25ml,成功建立了PP疾病模型。
2.感染猪的WBC、GRA及MON的数量及比例显著增高(P<0.01),LYM的数量及比例显著降低(P<0.01)。血清GLB含量显著增高(0.01 3.感染猪的RBC-C3bRR极显著升高(P<0.01),RBC-ICR降低；外周血CD3+T细胞降低、CD3+CD4+T细胞极显著降低(P<0.01)、CD3+CD8+T细胞和CD4+/CD8+显著降低(0.01 4.感染猪的肺组织除CAT活力显著增高(0.01 5.猪类全基因组芯片表达谱的结果与分析：(1)芯片扫描样点均匀规则,信号强度高,信噪比高,探针特异性强,芯片检出率高,集中在53%-74%区间。(2)用T检验方法分析得到感染与非感染APP猪肺及肺门淋巴结的差异表达基因及其交集。(3)SOM分析将差异表达基因聚为9类,涉及到免疫增强、Toll-like受体信号通路、磷脂酰肌醇信号系统通路,粘着连接通路等过程。样本HCL将相同组织聚为一类,即肺组织和肺门淋巴结样本各自聚类；同组织(肺或淋巴)感染与非感染样本各自聚类。(4)PCA分析显示：差异基因在同组织以疾病动物高于健康动物为主要表达模式；在同一个体以肺门淋巴结高于肺组织为主要表达模式。(5)基于GO功能分类的GCT结果显示：在肺组织,基因变异程度达到显著和极显著(PErmineJ<0.05(?)口0.01)的GO分类89个,与免疫有关的27个。在肺门淋巴结,基因变异程度达显著和极显著(PErmineJ<0.05和0.01)的GO分类有272个,与免疫有关的50个。(6)GSEA结果显示：基因在与感染有关的,如Fcepsilon RI信号传导途径、NOD-like受体信号通路、急性骨髓性白血病、癌症、孕酮介导卵母细胞成熟、剪接体、第Ⅱ型糖尿病、类固醇激素生物合成等pathway中显著富集,并得知这些pathway发挥调控作用的方向性(上调或下调)。Leading edge analysis发现多个调控机体抗感染与组织损伤修复可能具有重要或潜在作用的基因,在不同的pathway中多次出现。(7)基因芯片的可靠性评估结果显示：基因芯片内的变异系数为2.57%-5.50%。qRT-PCR验证结果显示,两种不同实验方法检测结果为正相关,相关系数平均为0.861±0.127。
结论：
1.利用Agilent猪类全基因组芯片研究感染APP猪肺脏及肺门淋巴结的表达谱,筛选出了对猪免疫、抗感染及组织损伤可能有重大影响和重要调控作用的基因31个以及有较大研究价值的pathway19个,初步揭示了APP感染对猪肺及肺门淋巴结损伤的分子机理。
2.感染APP猪的细胞免疫和体液免疫功能下降,红细胞免疫功能增强；总抗氧化能力下降。
Objectives:Porcine pleuropeumonia has caused very serious loss and damage in the modern high-stocking density large-scale breeding farms. The etiology, epidemic characteristics, test methods and other research of porcine pleuropneumonia has been a focus of attention of the various countries'researchers and scholars; while the systematic studies of tissue damage caused by bacteria infection and immune-related indexes on the body of pig are rare. Therefore, this study aimed at:(1) Establishing the pleuropneumoniae pathological model of artificial infection;(2) Observating and studying the swine symptoms, blood indicators and biochemical parameters, antioxidant indicators, immune parameters (red blood cell immunity, T cell-mediated immunity, humoral immunity and cytokine levels) and pathological changes post-infection with APP;(3) Using the Agilent swine genome-wide microarray containing43,603probes to detect the changes in gene expression of infected pigs'lungs and hilar nodes, to explore the molecular mechanisms of injury infection.
Results:
1. The porcine pleuropneumonia disease model were builded succesfully by nasal spraying0.25ml diluent contained3.5-4×107CFU/ml APP every kilogram weight.
2. The number of leukocytes, and the numbers and the percentages of granulocyte and monocytes were all increased significantly (P<0.01) while the number and the percentage of lymphocytes were decreased significantly (P<0.01). The content of GLB in infected-APP pigs serum was increased significantly (0.01 3. The ratios of RBC-C3b rosette were increased significantly (P<0.01) and the ratios of IC rosette were decreased (P<0.01); the percentage of CD3+T cells in the peripheral blood and CD3+CD4+T cells were decreased extremely significantly (P<0.01) while the percentage of CD3+CD8+T cells and CD4+/CD8+were decreased significantly (0.01 4. In the lung tissues of infected-APP pig, SOD and GSH-PX activities were decreased (P<0.01), and CAT activities were increased (0.01 5. The main results of pig genome-wide expression analysis of the chip are as follows:(1)Pig-chip scan results were showed that the sampling point was uniform and inerratic, the signal intensity was high, the signal to noise ratio was high, the specificity of probe was strong, detection rate of chip was high, and it concentrated in the range53%～74%.(2) T-tests were used to detect the differentially expression with a pig's lung and HLN in infected and non-infected:having got the DE genes and these intersection in the infected and non-infected lung and HLN.(3) The SOM of DE genes revealed that the genes were classified into9kinds, included the processes of immune enhancement, Toll-like receptor signaling pathway, phosphatidylinositol signaling pathway, and adhesion connecting pathway. The HCL of DE genes revealed that lung samples and HLN samples each clustered together; infected and non-infected samples of the same tissue each clustered together.(4) PCA revealed that the main expression pattern in the same kind of tissue was that DE genes in disease animals were higher than those in healthy ones; while in the same individual, DE genes in lymphoid tissue were higher than those in lung tissue.(5) GCT based on GO analysis revealed that89GO terms that the degree of genetic variation reached significant and highly significant (PErmineJ<0.05and0.01) were found in lung, including27GO terms related with immune.272GO terms that the degree of genetic variation reached significant and highly significant (PErmineJ<0.05and0.01) were found in HLN, including50GO terms related with immune.(6) GSEA revealed that genes were enriched significantly in a number of infection-related pathway including Fc epsilon RI (one of IgE receptors) signaling transduction pathway, NOD-like receptor signaling pathway, acute myeloid leukemia, cancer, progesterone-mediated oocyte maturation, spliceosome, Ⅱ diabetes, steroid hormones biosynthesis, and have got the direction (upward or downward) of these pathway that plays a regulatory role in each group. By Leading edge analysis found several potential genes appeared in a different pathway in the high-frequency and may had an important role in the regulation of body resistance to infection and the repair of damaged tissue by Leading edge analysis found.(7) The reliability evaluation results showed that the largest relative Standard Deviation (RSD) was5.50%and the smallest RSD was only2.57%in the gene chip. QRT-PCR verification results showed that there were a positive correlation between two detection results from different experimental methods in lung and lymphoid tissue and correlation coefficient averaged at0.861±0.127.
Conclusion and innovation
1. Initially reveal the molecular basis of the APP infection damage to the lung and HLN by using the Agilent pigs genome-wide microarray expression profiling.31genes were selected which might have a significant impact and important role for the pigs' immune, anti-infection and anti-tissue damage, as well as19pathways of greater research value.
2. The cellular immune and humoral immune function of the infected-APP pigs decreased, while the erythrocyte immune function increased. The T-AOC of infected-APP pigs were decreased.
PP在高密度饲养的现代大型规模化养猪场所造成的损失及危害相当严重。PP的病原学、流行特点及检测方法等一直是国内外研究人员和学者关注的焦点；关于APP感染对猪机体组织损伤及免疫相关的系统研究较为少见。本研究旨在：(1)建立PP人工感染疾病模型；(2)监测感染APP后,猪的病症、血液常规及生化指标、抗氧化功能、免疫状况(红细胞免疫、T细胞免疫与体液免疫及细胞因子水平)及病理变化；(3)利用含43,603个探针的Agilent猪类全基因组芯片检测感染APP猪的肺和肺门淋巴结基因表达谱变化,探索感染组织损伤的分子机制。
结果：
1.采用鼻腔气雾法,按猪每千克体重鼻腔喷雾含APP3.5-4×107CFU/ml的APP(I型)稀释液0.25ml,成功建立了PP疾病模型。
2.感染猪的WBC、GRA及MON的数量及比例显著增高(P<0.01),LYM的数量及比例显著降低(P<0.01)。血清GLB含量显著增高(0.01
结论：
1.利用Agilent猪类全基因组芯片研究感染APP猪肺脏及肺门淋巴结的表达谱,筛选出了对猪免疫、抗感染及组织损伤可能有重大影响和重要调控作用的基因31个以及有较大研究价值的pathway19个,初步揭示了APP感染对猪肺及肺门淋巴结损伤的分子机理。
2.感染APP猪的细胞免疫和体液免疫功能下降,红细胞免疫功能增强；总抗氧化能力下降。
Objectives:Porcine pleuropeumonia has caused very serious loss and damage in the modern high-stocking density large-scale breeding farms. The etiology, epidemic characteristics, test methods and other research of porcine pleuropneumonia has been a focus of attention of the various countries'researchers and scholars; while the systematic studies of tissue damage caused by bacteria infection and immune-related indexes on the body of pig are rare. Therefore, this study aimed at:(1) Establishing the pleuropneumoniae pathological model of artificial infection;(2) Observating and studying the swine symptoms, blood indicators and biochemical parameters, antioxidant indicators, immune parameters (red blood cell immunity, T cell-mediated immunity, humoral immunity and cytokine levels) and pathological changes post-infection with APP;(3) Using the Agilent swine genome-wide microarray containing43,603probes to detect the changes in gene expression of infected pigs'lungs and hilar nodes, to explore the molecular mechanisms of injury infection.
Results:
1. The porcine pleuropneumonia disease model were builded succesfully by nasal spraying0.25ml diluent contained3.5-4×107CFU/ml APP every kilogram weight.
2. The number of leukocytes, and the numbers and the percentages of granulocyte and monocytes were all increased significantly (P<0.01) while the number and the percentage of lymphocytes were decreased significantly (P<0.01). The content of GLB in infected-APP pigs serum was increased significantly (0.01
Conclusion and innovation
1. Initially reveal the molecular basis of the APP infection damage to the lung and HLN by using the Agilent pigs genome-wide microarray expression profiling.31genes were selected which might have a significant impact and important role for the pigs' immune, anti-infection and anti-tissue damage, as well as19pathways of greater research value.
2. The cellular immune and humoral immune function of the infected-APP pigs decreased, while the erythrocyte immune function increased. The T-AOC of infected-APP pigs were decreased.
引文
[1]杨旭夫,彭发泉.我国猪胸膜肺炎嗜血杆菌感染症的确定和诊断[J].畜牧兽医学报,1990,(03)：243-245.
[2]Taylor DJ. Actinobacillus pleuropneumoniae[M]. In:Straw BE, D'Allaire S, Mengeling WL and Taylor DJ, editor. Diseases of swine.8th. Vol.26. Ames Iowa Iowa State University Press,1999. pp.343-354.
[3]Hoflack, G, D Maes, B Mateusen, et al. Efficacy of Tilmicosin Phosphate (Pulmotil Premix) in feed for the treatment of a clinical outbreak of Actinobacillus pleuropneumoniae infection in growing-finishing pigs[J]. J. Vet. Med. B.2001,48:655-664.
[4]Blackall, P.2001. Pleuropneumonia in pigs. Agency for Food and Fibre Sciences. Deartment of Primary Industries, Queensland Government. Available:http://www. dpi. qld. gov. au/pigs/4463. html. Accessed Jan.13,2004.
[5]唐慧稳.猪传染性胸膜肺炎研究现状[J].畜牧兽医杂志,2003,4(22)：19.
[6]陈如明,谢鸣星,李云峰,等.鲁豫地区驻军猪传染性胸膜肺炎血清学调查[J].中国畜禽传染病,1990,(4)：38-40.
[7]汤万倾.我国猪传染性胸膜肺炎的危害及诊断防治研究[J].畜牧兽医科技息,2004(04)：18-19.
[8]Jacques M. Surface polysaccharides and iron-uptake systems of Actinobacillus pleuropneumoniae[J]. Can J Vet Res,2004,68(2):81-85.
[9]NIELSEN R, ANDERSEN L O, PLAMBECK T, et al. Serological characterization of Actinobacillus pleuropneumoniae biotype 2 strains isolated from pigs in two Danish herds [J]. Vet Microbiol,1997,54:35-46.
[10]BLACKALL P J, KLAASEN H L, VAN DEN BOSCH H, et al. Proposal of a new serovar of Actinobacillus pleuropneumoniae:serovar 15[J]. Vet Microbiol,2002,84:47-52.
[11]Chang Y, Yong R, Struck D K. Cloning and characterization of a hamolysin gene from Actinobacillus pleuropneumoniae[J]. DNA,1989, (8):635-647.
[12]Sebunya T N, Saunders J R. Haemophilus pleuropneumoniae infection in swine:a review. J Am Vet Med Assoc,1983,182:1331-1337.
[13]郑芝.某猪场暴发猪接触传染性胸膜肺炎的诊断报告.中国畜禽传染病,1987,4：33-34
[14]高齐瑜,刘尚高,马理武.猪嗜血杆菌胸膜肺炎病实验诊断和病理形态学观察.中国兽医杂志,1988,14：5-7
[15]吕盛喜,贡东栋,马健.猪接触传染性胸膜肺炎的诊断与防治.中国兽医杂志,1992,18：17
[16]杨旭夫,苑士祥,杨留战,等.我国部分地区猪传染性胸膜肺炎病的调查。中国兽医杂志,1993,19：5-6
[17]周以凤,吴硕显.上海市猪胸膜肺炎的血清学调查.中国兽医科技,1993,9：14-15
[18]徐金生,黄宝春,萧军.进口美国种猪首次检出猪嗜血杆菌胸膜肺炎抗体。动物检疫,1988,1：11-13
[19]沈特来,毛少华,俞少麟.从加拿大进口种猪中首次检出猪传染性胸膜肺炎嗜血杆菌病.动物检疫,1989,2：21-23
[20]龚云登,杨长松.鄂西南地区猪传染性胸膜肺炎血清学调查[J].中国兽医杂志,2010,(2)：93-94.
[21]鄢志勇,龙清孟,龚菲,等.种猪场猪传染性胸膜肺炎的血清学监测及防控[J].中国畜牧兽医,2009(12)：135-136.
[22]赖玉兰,田明星,曹三杰,等.猪传染性胸膜肺炎放线杆菌血清5a型ApxⅣA全基因的序列测序及其分子特征[J].四川农业大学学报,2009,27(4)：479-486.
[23]唐文山,李昕.猪传染性胸膜肺炎研究进展[J].动物医学进展,2008,29(2)：98-102.
[24]何启盖,吴斌,吴信明,等.猪接触传染性胸膜肺炎的防制现状.华中农业大学学报,2000,33(增刊)：58-62
[25]陈小玲,杨旭夫,朱士盛.猪传染性胸膜肺炎的流行现状和防制措施.中国兽医杂志,2001,37：33-35
[26]高作信主编.猪病学.北京：中国农业出版社,第三版,2001,178-179
[27]Willson P J, Falk G, Klashinsky S. Detection of Actinobacillus pleuropneumoniae infection in pigs. Can Vet J,1987,28:111-116
[28]Nicolet J. Actinobacillus pleuropneumoniae. In:Leaman A D, Straw B E, Mengeling S, Allaire SD, Taylor D J eds., Diseases of swine. Ames:Iowa State University Press,1992,401-408.
[29]Torremorell M, Pijoan C, Janni IL Walker R, et al. Airborne transmission of Actinobacillus pleuropneumoniae and porcine reproductive and respiratory syndrome virus in nursery pigs. Am J Vet Res,1997,58:828-832.
[30]Vigre H, Angen O, Barfod K La, et al. Transmission of Actinobacillus pleuropneumoniae in pigs under field-like conditions:emphasis on tonsillar colonization and passively acquired colostral antibodies. Vet Microbiol,2002,89:151-159.
[31]尚丽娟.猪传染性胸膜肺炎的防治[J].中国畜牧兽医,2008,35(2)：107-108.
[32]斯特劳[美],阿莱尔[加],蒙加林[美],泰勒[英]主编；赵德明,张中秋,沈建中主译.猪病学(第八版).北京：中国农业大学出版社,2000,357-367
[33]Cruijsen T,Van Leengoed L A, Kamp E M, et al. Suseeptibility to Actinobacillus pleuropneumoniae infection in pigs from all endemically infected herd is related to the presence of toxin-neutralizing antibodies. Vet Microbiol,1995,47:219-228.
[34]Dom P, Haesebrouck F, Kamp E M, et al. NAD-independent Actinobacillus pleuropneumoniae strains:production of RTX toxins and interactions with porcine phagocytes. Vet Microbiol,1994.39:205-218
[35]Chiers K Haesebrouck F, Van Overbeke I, et al. Ducatelle IL Early in vivo interaction of Actinobacillus pleuropneumoniae with tonsils of pigs. Vet Microbiol,1999,68:301-306.
[36]Jacques M, Belanger M, Rov G, et al. Adherence of Actinobacillus pleuropneumoniae to porcine tracheal epithelial cells and frozen lung sections. Vet Microbiol,1991,27:133-143.
[37]Mulks M H, Moxon E R,Bricker J, et al. Examination of Haemophilus pleuropneumoniae for immunoglobulin A protease activity. Infect lmmun,1984,45:276-277
[38]Negrete-Abascal E, Reyes M E, Garcia R M, et al. Flagella and Motility in Actinobacillus pleuropneumoniae. J Bacteriol,2003,185:664-668
[39]Bilinski T. Oxygen toxicity and microbial evolution. Biosystems,1991,24:305-312.
[40]Langford P R, Loynds B M, Kroll J S. Cloning and molecular characterization of Cu,Zn superoxide dismutase from Actinobacillus pleuropneumoniae. Infect lmmun,1996,64:5035-5041.
[41]Bosse J T, Macinnes J I. Urease activity may contribute to the ability of Actinobacillus pleuropneumoniae to establish infection. Can J Vet Res,2000,64:145-150.
[42]毛志亮,王孝荣,李翠苹,等.猪传染性胸膜肺炎的诊断与防治[J].畜牧与兽医,2008,40(3)：112.
[43]逯忠新.猪传染性胸膜肺炎.见：陈焕春主编,规模化猪场疫病监控与净化。北京：中国农业出版社,2000：273-276
[44]Jacobsen M J, Nielsen J P.Development and evaluation of a selective and indicative medium for isolation of Actinobacillus pleuropneumoniae from tonsils. Vet Microbiol,1995,47:191-197.
[45]Sidibe M, Messier S, Lariviere S, et al. Detection of Actinobacillus pleuropneumoniae in the porcine upper respiratory tract as a complement to serological tests. Can J Vet Res,1993,57: 204-208
[46]张蓉蓉,粱旺望,方兵兵,等.免疫磁珠技术分离猪传染性胸膜肺炎放线杆菌.畜牧兽医学报,2008,39：343-348
[47]Fittipaldi N, Broes A, Harel J, et al. Evaluation and field validation of PCR tests for detection of Actinobacillus pleuropneumoniae in subclinically infected pigs. J Clin Microbiol,2003.41: 5085-5093.
[48]Chiers K,Van Overbeke I, Donne E, et al. Detection of Actinobacillus pleuropneumoniae in cultures from nasal and tonsillar swabs of pigs by a PCR assay based on the nucleotide sequence of a dsbE-like gene. Vet Microbiol,2001,83:147-159.
[49]Gram T, Ahrens P, Nielsen J P. Evaluation of a PCR for detection of Actinobacillus pleuropneumoniae in mixed bacterial cultures from tonsils. Vet Microbliol,1996,51:95-104.
[50]Gram T, Ahrens P, Andreasen M, et al. An Actinobacillus pleuropneumoniae PCR typing system based on the apx and omlA genes-evaluation of isolates from lungs and tonsils of pigs. Vet Microbiol,2000,75:43-57.
[51]逯忠新,杨学山,赵卫平,等.猪胸膜肺炎放线杆菌PCR检测方法的建立.中国兽医科技,2004,34：6-8
[52]王牟平,刘思国,王春来,等.猪传染性胸膜肺炎PCR诊断方法的建立.中国预防兽医学报,2003,25： 305-309
[53]刘军发,何启盖,陈焕春,等.用PCR检测猪胸膜肺炎放线杆菌.华中农业大学学报,2001,20：156-158
[54]李鹏,马艳娇,夏伟,等.猪肺炎支原体、多杀性巴氏杆菌和猪胸膜肺炎放线杆菌三重PCR检测方法的建立[J].中国预防兽医学报,2010,(8)：607-610.
[55]徐景峨,蔡一鸣,王璇,等.猪传染性胸膜肺炎放线杆菌PCR检测试剂盒的研制[J].中国畜牧兽医,2010,(4)：209-211.
[56]Cho W S, Chae C. Expression of the apxIV gene in pigs naturally infected with Actinobacillus pleuropneumoniae. J Comp Pathol,2001,125:34-40.
[57]Cho W S , Chae C. PCR detection of Actinobacillus pleuropneumoniae apxIV gene in formalin-fixed paraffin-embedded lung tissues and comparison with in situ hybridization. Lett Appl Microbiol,2003,37:56-60
[58]Rosendal S, Carpenter D S,Mitchell W R, et al. Vaccination against pleuropneumonia of pigs caused by Haemophilus pleuropneumoniae. Can Vet J 1981,22:34-35
[59]王贵平.猪传染性胸膜肺炎免疫荧光和PCR诊断方法研究及apxIVA的克隆与序列分析。[硕士学位论文].武汉：华中农业大学图书馆,2004
[60]Hoffman L. Actinobacillus(Haemophilus) pleuropneumoniae:use of coagglutination and complement fixation to determine the relationship between presence of organism and antibody titer in slaughter house pigs. J Vet Diagn Invest,1989,1:12-15.
[61]Nielsen R. Haemophilus pleuropleumoniae(Actinobacillus pleuropleumoniae)serotype 8,3 and 6 serological response and cross immunity in pigs. Nord vet Med,1985,37:217-227.
[62]Lombin L H, Rosendal S and Mitchell W R. Evalution of the complement fixation test for the diagnosis of pleuropneurnoniae of swine caused by Haemophilus pleuropneumoniae. Can J comp Med,1982,46:109-114.
[63]Mittal K R, Higgins R, Lariviere S. Evaluation of slide agglutination and ring precipitation tests for capsular serotyping of Haemophilus pleuropneumoniae. J Clin Microbiol,1982,15:1019-1023
[64]逯忠新,鲁炳义用间接血凝试验检测猪传染性胸膜肺炎.中国兽医科技,1999,29-25-27
[65]Mittal K R, Higgins R, Lariviere S, et al. A 2-mereaptoethanol tube agglutination test for diagnosis of Haemophilus pleuropneumoniae infection in pigs. Am J Vet Res,1984,45:715-719.
[66]Mittal K R, Higgins R, Lariviere S. An evaluation of agglutination and coagglutination techniques for serotyping of Haemophilus pleuropneumoniae isolates. Am J Vet Res,1987,48:219-226
[67]Rapp V J, Ross R F, Erickson B Z.Serotyping of Haemophilus pleuropneumoniae by rapid slide agglutination and indirect fluorescent antibody tests in swine. Am J Vet Res,1985,46:185-192
[68]Gunnarsson A, Hurvell B, Biberstein E L. Serologic studies of Haemophilus parahaemolyticus (pleuropneumoniae):antigenic specificity and relationship between serotypes. Am J Vet Res, 1978,39:1286-1292
[69]Gunnarsson A. Serological studies on porcine strains of Haemophilus parahaemolyticus (pleuropneumoniae):extraction of type-specific antigens. Am J Vet Res,1979,40:469-472.
[70]Giese S B, Stebaek E, Nielsen R. Identification of Actinobacillus pleuropneumoniae serotype 2 by monoclonal or polyclonal antibodies in latexagglutination tests. Acta Vet Scand,1993,34: 223-225.
[71]Inzana T J. Simplified procedure for preparation of semitized latex particles to detect capsular polysaccharides:application to typing and diagnosis of Actinobacillus pleuropneumoniae. J Clin Microbiol,1995,33:2297-2303.
[72]Hommez J, Devriese L A, Castryck F, et al. Slide precipitation:a simple method to type Actinobacillus(Haemophilus)pleuropneumoniae. Vet Microbiol,1990,24:123-126.
[73]Bosse J L, Johnson RP, Rosendal S. Serodiagnosis of pleuropneumonia using enzymed-linked immunosorbent assay with capsular polysaccharide antigen of Actinobacillus pleuropneumoniae serotype 1,2,5 and 7. Can J Vet Res,1990,54:427-431.
[74]Radacovici S,Lallier R, Lariviere S, et al.Biochemical characterization of an antigenic saline extract of Actinobacillus pleuropneumoniae serotype 5 and identification of a serotype-specific antigen for ELISA serodiagnosis. Vet Microbiol,1992,30:369-385
[75]Radacovici S,Gottschalk M,Dubreuil J D.Recovery of long-chain lipopolysaccharides from liquid culture of Actinobacillus pleuropneumoniae(serotype 5)for ELIS A serodiagnosis. Vet Res,1995, 26:63-67
[76]Gottschalk M, De LaSalle F, Radacovici S, et al. Evaluation of long chain lipopolysaccharides(LC-LPS) of Actinobacillus pleuropneumoniae serotype 5 for the serodiagnosis of swine pleuropneumonia. Vet Microbiol,1994,38:315-327
[77]Nielsen N, Plambeck T, Foged N T. Blocking enzyme—linked immunosorbent assay for detection of antibodies to Actinobacillus pleuropneumoniae serotype 2. J Clin Microbiol,1991,29: 794-797
[78]Nielsen R, Plambeck T, Foged N T. Blocking enzyme-linked immunosorbent assay for detection of antibodies against Actinobacillus pleuropneumoniae serotype 8. Vet Microbiol,1993,34:131-138
[79]Andresen L O, Klausen J, Barfod K Sorensen V. Detection of antibodies to Actinobacillus pleuropneumoniae serotype 12 in pig serum using a blocking enzyme-linked immunosorbent assay. Vet Microbiol,2002,89:61-67
[80]Leiner G, Franz B, Strutzberg K, et al.A novel enzyme-linked immunosorbent assay using the recombinant Actinobacillus pleuropneumoniae ApxII antigen for diagnosis of pleuropneumonia in pig herds. Clin Diagn Lab Immunol,1999,6:630-632.
[81]Nielsen R,van den Bosch J F, Plambeck T, et al. Evaluation of an indirect enzyme-linked immunosorbent assay(ELISA)for detection of antibodies to the Apx toxins of Actinobacillus pleuropneumoniae. Vet Microbiol,2000, 71:81-87
[82]Dreyfus A, Schaller A, Nivollet S, et al. Use of recombinant ApxIV in serodiagnosis of Actinobacillus pleuropneumoniae infections,development and prevalidation of the ApxIV ELISA. Vet Microbiol,2004,99:227-238.
[83]黄红亮,周锐,陈美玲,等.胸膜肺炎放线杆菌apxIVA基因的克隆与表达及间接ELISA方法的建立.生物工程学报,2005,21：294-299
[84]Hu D H, Hart H Y, Zhou R, et al. Gold(III) enhanced chemiluminescence immunoassay for detection of antibody against ApxIV of Actinobacillus pleuropneumoniae. Analyst,2OO8,133: 768-773.
[85]Sheng Z H,Han H Y,Hu D H, et al. Quantum dots-gold(Ⅲ)-based indirect fluorescence high-throughput screening of APP. Chem Comm,2009, DOI:10.1039/b822921j
[86]Pol J M, Van Leengoed L A, Stockhofe N, et al. Dual infections of PRRSV/influenza or PRRSV/Actinobacillus pleuropneumoniae in the respiratory tract. Vet Microbiol,1997,55: 259-264
[87]张彦明,张耀相,刘春花,等.胸膜肺炎放线杆菌的分离鉴定及免疫预防.中国兽医科技,2002,32：21-22
[88]李清艳,翟向和,钟秀会,等.附红细胞体自然感染病猪血浆NO、SOD和MDA的变化[J].动物医学进展,2005,26(2)：92-94.
[89]孙存普,张建中,段绍瑾.自由基生物学导论[M].安徽合肥,中国科学技术出版社,1999.
[90]毕晋明,郑姗姗,张敏红,等.日粮中添加不同浓度吡啶甲酸铬对生长肥育猪氧化作用的影响[J].动物营养学报,2008,20(5)：561-566.
[91]龙建纲,王学敏,高宏翔,等.丙二醛对大鼠肝线粒体呼吸功能及相关脱氢酶活性影响[J].第二军医大学学报,2005,26(10)：1131-1135.
[92]莫简.自由基在生物体系中的作用：普通中间介质或非特异性调节剂[J].自由基生命科学进展,1999(7)：7-10.
[93]Vander Vliet A, Cross CE. Oxidants, nitrosants, and the lung[J]. Am J Med,2000,109(5): 398-421.
[94]李华强,史源,潘捷,等.内源性一氧化氮在感染性肺炎病理过程中的作用[J].第三军医大学学报,1999,21(3)：206-208.
[95]Yu J C, Jiang Z M, Li D M. Glutamine:a precusor of glutathione and its efect on liver[J]. World J Gastroentero,1999,5(2):143-146.
[96]Alverdy AM. Effects of glutamine-supplemented diets on immunology of the gut[J]. Parenter Enteral Nutr,1990,(14):109-113.
[97]赵晶,康世良.健康仔猪口服亚硒酸钠后血液硒浓度与抗氧化系统动态变化规律的研究[J].畜牧兽医学报,2003,34(6)：554-557.
[98]Abu-khaderAA, BihoYY. Exposure of human meutrophils to oxygen redicals causes loss of deformability. lipid peroxidation, protein degradation, respiratory burst activation and loss of migration[J]. Clin Hemorheol Microcircula,2002,27(1):57-66.
[99]李敏,崔伟,彭西,等.高铜对雏鸡脑组织抗氧化酶活性的影响[J].畜牧兽医学报,2010,41(2)：220-22
[100]Vukic Z. Gluteal gunshot wounds [J]. Mil Med,2000,165(3):237-239.
[101]llie N, Petricevic A, Tanfara S, et al. War-mjuriestothe chest[J]. Acta Chir Hung,1999, 38(1):43-47.
[102]Desjardins F, Balligand JL. Nitric oxide-dependent endothelial function and cardiovascular disease[J]. Acta Clin Belg,2006,61(6):326-334.
[103]Yetik-Anacak G, Catravas JD. Nitric oxide and the endothelium:history and impact on cardiovascular disease[J]. Vaseul Pharmacol 2006,45(5):268-276.
[104]Pheng LH, Francoeur C, Denis M. The involvement of nitric oxide in a mouse model of adult respiratory distress syndrome [J]. Inflammation,1995,19:599-610.
[105]Bloomfield GL, Holloway S, Ridings PC, et al. Pretreatment with inhaled nitric oxide inhibits neutrophil migration and oxidative activity resulting in attenuated sepsis-induced acute lung jnjury[J]. Crit Care med,1997,25:584-593.
[106]Gaston B, Drazen JM, Loscalzo J, et al. The biology of nitrogen oxides in the airways[J]. Am J Respir Crit Care Med,1994,149:538-51.
[107]Btmser, Minlseh A, Fleming F, et al. Mechanism of nitric oxidere lease from the vascular endothelium. Circulation.1993,97(supp1):18.
[108]Bingisser R M. Hoh P G. Immunomod ulating mechanisms in the lower respiratory tract:nitric oxide mediated interactions between alveolar macmphages, epithelial cells and T-cells[J]. Swiss Med Wkly,2001; 131(13-14):171-179.
[109]刘晓宇,高和.一氧化氮对呼吸系统炎症与病原微生物影响研究进展[J].国际呼吸杂志,2007,27(5)：338-341.
[110]王宁飞,蔡富娟,刘帅,等.过表达Rab7基因对LPS和PolyI:C活化的巨噬细胞RAW264.7中iNOS/NO的影响[J].中国免疫学杂志,2011,27(6)：497-501.
[111]赵丽,杨帆,彭西,等.高铜对雏鸭肝羟自由基和一氧化氮产生的影响.中国兽医科学2008.38(09)：787-790.
[112]Bian K, Harari Y, Zhong M. Down-regulation of inducible nitric-oxide synthase (NOS-2) during Parasite-induced gut Inflammation:a Path to identify a selective NOS-2 inhibitor. Mol Pharmacol, 2001,59:939-947.
[113]程安玮,金征宇,万发春.甘草多糖xCd,鼠腹腔巨噬细胞NO、iNOS及iNOS mlLNA表达的影响[J].食品科学,2009,30(21)：351-352.
[114]蒋波,楚正绪.一氧化氯与炎症[J].国外医学一生理病理科学与临床分册,1998,18(1)：44-47.
[115]姜勇.内毒素激活内皮细胞的信号机制的研究进展[J].中华医学杂志,1999,79：76-79.
[116]Selye. H A. Syndrome produced by diverse nocuous agents [J]. Nature,1936:32.
[117]Mader T L. Environm ental stress in confined beef cattle [J]. Anim Sci,2003,81:110-119.
[118]Raymond C R, Wilkie B N. Th-1/Th-2 type cytokine profiles of pig T-cells cultured with antigen-treated monocyte-derived dendritic cells[J]. Vaccine,2004,22:1016-1023.
[119]G. B. Toews, Cytokines and the lung, Eur. Respir. J. Suppl.34 (2001), pp.3s-17s.
[120]Duh EJ, WJ Maury, TM Folks, et al. Tumor necrosis factor alpha activates human immunodeficiency virus type 1 through induction of nuclear factor binding to the NF-kappa B sites in the long terminal repeat. Proc Natl. Acad Sci USA,1989,86:5974-5978.
[121]Kronke, M., S. Schutze, P.Scheurich, et al. TNF signal transduction and TNF-responsive genes[J]. Immunol. Ser,1992,56:189-216.
[122]Lowenthal, J. W., D. W. Ballard, et al. Tumor necrosis factor alpha induces proteins that bind specifically to kappa B-like enhancer elements and regulate interleukin 2 receptor alpha-chain gene expression in primary human Tlymphocytes. Proc. Natl. Acad. Sci. USA,1989,86:2331-2335.
[123]Osborn, L, S. Kunkel, G. J. Nabel. Tumor necrosis factor alpha and interleukin 1 stimulate the human immunodeficiency virus enhancer by activation of the nuclear factor kappa B. Proc. Natl. Acad. Sci. USA,1989,86:2336-2340.
[124]Schutze S, K.Potthoff, T. Machleidt, et al. TNF activates NF-kappa B by phosphatidylcholine-specific phospholipase C-induced "acidic" sphingomyelin breakdown[J]. Cell,1992,71:765-776.
[125]Bussolino.F, G. Camussi, C. Baglioni. Synthesis and release of platelet-activating factor by human vascular endothelial cells treated with tumor necrosis factor or interleukin 1 alpha[J]. J. Biol. Chem,1988,263:11856-11861.
[126]Libby.P, J.M Ordovas, K.R. Auger, et al. Endotoxin and tumor necrosis factor induce interleukin-1 gene expression in adult human vascular endothelial cells[J]. Am. J. Pathol,1986, 124:179-185.
[127]PP Nawroth, I Bank, D Handley, et al. Tumor necrosis factor/cachectin interacts with endothelial cell receptors to induce release of interleukin 1[J]. J. Exp. Med,1986,163:1363-1375.
[128]Pober J.S, M.Gimbrone, LA Lapierre, et al. Overlapping patterns of activation of human endothelial cells by interleukin 1, tumor necrosis factor, and immuneinterferon[J]. J. Immunol, 1986,137:1893-1896.
[129]Molnar L, Berki T, Hussain A, et al. Detection of TNFalpha expression in the bone marrow and determination of TNFalpha production of peripheral blood mononuclear cells in myelodysplastic syndrome[J]. Pathol Oncol Res.2000;6:18-23.
[130]Chrysant SG. The path physiologic role of the brain Renin-Angiotensin system in stroke protection:clinical implications [J]. J Clin Hypertens(Greenwich),2007,9(6):454-459.
[131]Di Giovine F S, Nuki GL. Tumor nerosis factor in synovial exudates. An Rheum Dis,1988,47: 768-772.
[132]Maier SF, Watkins LR. Cytokines for psychologists:implications of bidirectional immune-to-brain communication for understanding behavior, mood, and cognition[J]. Psychol Rev 1998,105:83-107.
[133]Dinarello CA. Biologic basis for interleukin-1 in disease[J]. Blood,1996,87:2095-2147.
[134]李戎,闰智勇,李文军,等.肺纤维化发生与防治机制的重要因素-细胞因子作用机制研究[J].中国中医药,2004,6：23.
[135]Marz W, Wieland H. HMG-CoA reeducates inhibition:antiinflammatory effects beyond lipid lowering[J]. Herz,2000,25(2):117-125
[136]王光辉,陈传兵,王献武.支气管肺炎患儿治疗前后血清IGF.IL-I、IL-6、1L-8、和hs-CRP检测的临床意义[J].放射免疫学杂志,2009,22(4)：337-338.
[137]陈侃侃.肺炎支原体肺炎患儿血清hs-CRP、IL-6和IL-8检测的临床意义[J].放射免疫学杂志,2011,24(3)：266-267.
[138]Nagahori T, Uohi M, Matsumoto K, et al. Interferon-gitnma upregulates the C-Met/hepatocyte growth factor receptor expression in alveolar epithelial cells[J]. Am J Respir Cell Mol Biol,1999, 21:490-497.
[139]Bajwa EK, Ayas NT, Schuher M, et al. Interferon-lb therapy in idiopathic pulmonary fibrosis:a metaanalysis[J]. Chest,2005,128:203-206.
[140]Cantrell DA, Smith KA. The interleukin-2 T-cell system:a new cell growth model[J]. Science,1984,224 (4655):1312-1316.
[141]Smith KA. Interleukin-2:inception, impact, and implications[J]. Science,1988,240(4856): 1169-1176.
[142]金伯泉.医学免疫学[M].第5版.北京：人民卫生出版社,2008：60-65,105-107.
[143]DOOMS H, KAHN E, KNOECHEL B, et al. IL-2 induces a competitive survival advantage in T lymphocytes[J]. J Immunol,2004,172(10):5973-5979.
[144]李爱华,曹祥山.IL-2与CD4+CD5+调节性T细胞的相关研究进展[J].医学综述,2007,13(9)：652-653.
[145]Trojanowska M. Role of PDGF in fibrotic diseases and systemic sclerosis[J]. Rheumatology, 2008,47(5):2-4.
[146]卫张蕊,周国锋.炎症细胞和细胞因子在肺纤维化中作用的研究进展[J].细胞与分子免疫学杂志,2005,21(Supp1)：85-87.
[147]Li X, Hu Y, Jin Z, et al. Silica-induced TNF-alpha and TGF-betal expression in RAW 264.7 cells are dependent on Src-ERK/AP-1 pathways[J]. Toxicol Mech Methods,2009,19(1):51-58.
[148]姚武,王治明,王绵珍,等.煤工尘肺患者血清细胞因子及氧化损伤水平研究[J].四川大学学报(医学版),2005；36(4)：510-512.
[149]Santana A, Saxena B, Nancy A, et al. Increased expression of TGF-B1 in bleomycin-induced pulmonary fibrosis[J]. Am J Respir Cell Mol Biol,1995,13(1):34-44.
[150]Gulumian M, Borm PJ, Vallyathan V, et al. Mechanistically identified suitable biomarkers of exposure, effect, and susceptibility for silicosis and coal-worker's pneumoconiosis:a comprehensive review[J]. J Toxicol Environ Health B Crit Rev,2006,9(5):357-395.
[151]Petersen H H, Nielsen J P, Heegaard P M H. Application of acute phase protein measurements in veterinary clinical chemistry [J]. Veterinary Research,2004,35:163-187.
[152]Malle E, De Beer F C. Human serum amyloid A (SAA) protein:a prominent acute-phase reactant for clinical practice. Eur J Clin In-vest,1996,26(6):427-435.
[153]Yip T, Chan J, Cho W, et al. Protein chip array profiling analysis in patients with severe acute respirator syndrome identified serum amyloid A protein as a biomarker potentially useful in monitoring the extent of pneumonia[J]. Clin Chem,2005,51:47-55.
[154]Kokubun M, Imafuku Y, Okada M, et al. Serum amyloid A (SAA) concentration varies among rheumatoid arthritis patients estimated by SAA/CRP ratio. Clin Chimica Acta,2005,360:97-102.
[151]Bing Z, Reddy SA, Ben Y, et al. Purification and eharaeterization of the serum amyloid A3 enhancer factor [J]. J Biol Chem,1999,274(35):24649.
[152]Boylan MT, Crockard AD, Duddy ME. Interferon-betala administration results in a transient increase of 8enlm amyloid A protein and C-reactive protein:comparison with other markers of inflammation[J]. Immunol Lett,2001,75(31):191.
[153]Volanakis JE. Human C-reactive protein:expression, structure, and function. Mol Immunol,2001, 38:189-197.
[154]叶应妩,王毓三,申子瑜.全国临床检验操作规程[M].南京：东南大学出版社,第3版,2006：590.
[155]陈侃侃.肺炎支原体肺炎患儿血清hs-CRP、IL-6和IL-8检测的临床意义[J].放射免疫学杂志,2011,24(3)：266-267.
[156]曹嫚,何少为.肺炎支原体患儿血清中TNF-α和Hs-CRP水平联合测定的临床意义[J].中外医学研究,2011,9(18)：24-24.
[157]Eckersall PD, Saini PK, McComb C. The acute phase of acid soluble glycoprotein-lacid glycoprotein, ceruplasmin, haptoglobin ang C-reactive protein in the pigin a transient increase of[J]. Vet Immunol Immunopathol,1996,51:377-385.
[158]Heegaard PMH, Klausen J, Nielsen JP, et al. The porcine acute phase response to infection witn Actinobacillus pleuropneumoniae. Haptoglobin, C-reactive portein, major acute phase portien and serum amyloid A protein are sensitive indicators of infection. Comp Biochem Physiol[J],1998, 119B:365-373.
[159]Lauritzen B, Lykkesfeldt J, Skaanild MT, et al. Putative biomarkers for evaluating antibiotic treatment:an experimental model of porcine Actinobacillus pleuropneumoniae infection[J]. Res Vet Sci,2003,74:261-270.
[160]Lauritzen B, Lykkesfeldt J, Skaanild MT et al. Putative biomarkers for evaluating antibiotic treatment:an experimental model of porcine Actinobacillus pleuropneumoniae infection[J]. Res Vet Sci,2003,74:261-270.
[161]Hulten C, Johansson E, Fossum C, et al. Interleukin-6, serum amyloid A and haptoglobin as marker of treaqtment efficacy in pigs experimentally infected with Actinobacillus pleuropneumoniae[J]. Vet J,2003,159:194-200.
[162]BRANDTZAEG P. Molecular and cellular aspects of the secretory immunoglobulin system [J]. A pmis,1995,103(1):1-19.
[163]MACPHERSON AJ, McCOYKD, JOHANSENFE, et al. The immune geography of IgA induction and function [J]. Mucosal Immunology,2008,1:11-22.
[164]WOOF J M, KERR M A. The function of immunoglobulin A in immunity [J]. J Pathol,206, 208(2):270-282.
[165]赵武述.免疫平衡研究及其临床意义[M].北京：科学出版社,2005：90.
[166]龚非力.医学免疫学[M].北京：科学出版社,2000：39.
[167]CRAWLEY A, WILKIE B N. Porcine Ig isotypes:function and molecular characteristics [J]. Vaccine,2003,21 (21-22):2911-2922.
[168]周武,汪德清.红细胞功能检测方法的研究进展[J].中国输血杂志,2010,23(8)：648-650.
[169]Nelson R A. The immune phenomenon: An immunologically specific reaction between microorganisms and erythrocyte leading to enhanced phagocytosis [J]. Science,1953,118:773.
[170]SIEGEL I, TIAN Liu-liu, NORBERT G. The red-cell immune system [J]. Lancet,1981,318(9): 556-559.
[171]Siegel I, Gleicher N. Red cell immune adherence (RCIA):its application in cancer and autoimmune disease. Immunol Commun.1981;10(4-5):433-449.
[172]郭峰,虞紫茜,赵中丰,等.红细胞免疫功能的初步研究[J].中华医学杂志,1982,62(12)：715-716.
[173]张德成,陈思义,李进昌,等.动物红细胞免疫功能的研究[J].畜牧兽医学报,1992,23(3)：285-288.
[174]王旭东,饶光荣,李正甫,等.11类哺乳类和鸟类动物红细胞免疫功能的对比研究[J].畜牧兽医学报.1995.26(6)：524-529.
[175]张德成,陈思义,吴润培,等.动物红细胞C3b受体测定研究[A].南京：南京大学出版社.1993.123-125.
[176]王旭东,饶家荣,王生奎.九种红细胞免疫功能研究分析和意义[A].南京：南京大学出版社.1993.125-127
[177]胡金川.红细胞免疫及其临床应用研究进展[J].国际检验医学杂志,2008,29(7)：621-622.
[178]彭益,陈作友.尿毒症患者血液透析对红细胞免疫功能与脂质过氧化关系的研究[J].中国健康月刊：学术版,2010,29(7)：30-32.
[179]夏礼斌,赵咏莉,华强,等.2型糖尿病肾病患者红细胞免疫功能研究[J].中国医药指南,2010,8(22)：7-8.
[180]赵红霞.手足口病患儿红细胞免疫功能与脂质过氧化关系的探讨[J].放射免疫学杂志,2010,23(3)：343-344.
[181]章惠刚,付美丽,赵沛光,等.病毒性心肌炎患者外周血红细胞免疫功能的变化和血清cTnl水平的相关性分析[J].放射免疫学杂志,2010(2)：203-205.
[182]侯海锋,包永占,李茜,等.当归对鸡红细胞免疫功能的影响[J].中国兽医杂志,2009(4)：54-56.
[183]李晓卉,殷中琼.青刺果黄酮对鸡红细胞免疫及外周血淋巴细胞免疫功能的影响[J].中国兽医杂志,2009(3)：43-44.
[184]伊鹏霏,付本懂,张森,等.蓝黄青口服液对鸡传染性喉气管炎疫苗免疫的影响[J].中国家禽,2009(5)：16-19.
[185]彭西,柏才敏,李敏,等.高硒对雏鸡红细胞免疫功能红细胞总数及血红蛋白含量的影响[J].中国兽医科学,2008,38(6)：535-539.
[186]刘福堂,李艳飞,顾庆云.铝中毒对蛋鸡白细胞和红细胞免疫功能的影响[J].中国畜牧兽医,2007,34(10)：70-72.
[187]方静,崔恒敏,彭西.锌中毒对天府肉鸭红细胞免疫功能的影响[J].中国兽医科技,2004,34(9)：34-36.
[188]崔恒敏,彭西,黎德兵,等.高锌对雏鹅红细胞免疫功能的影响[J].中国兽医学报,2004,24(6)：576-578.
[189]陈龙,殷定忠,庞训胜,等.雏鸡在感染IBDV后红细胞免疫功能变化的研究[J].畜牧兽医学报,1998,29(3)：280-284.
[190]李清艳,翟向和,吕建存,等.附红细胞体感染对猪红细胞免疫功能的影响[J].中国兽医科技,2004,34(4)：46-48.
[191]富艳玲,王德志,王晓旭,等.中药乳膏剂对乳房炎奶牛非特异性免疫和红细胞免疫功能的影响[J].中国兽医杂志,2008,44(7)：51-53.
[192]李睿文,宋春丽,李铁拴,等.康贝对隐性乳房炎奶牛红细胞免疫的影响[J].中国兽医杂志,2005,41(6)：23-25.
[193]王丽,剡根强,王静梅.绵羊自然感染附红细胞体对绵羊红细胞的影响[J].石河子大学学报：自然科学版,2006,24(1)：108-111.
[194]温立斌,何孔旺,杨汉春,等.类圆环病毒因子P1感染对猪外周血T淋巴细胞含量和增殖活性的影响[J].华北农学报,2009,24(B08)：1-4.
[195]屈雪琪,赵建增,梁智选,等.流式细胞术检测不同试验条件对猪T淋巴细胞亚群的影响[J].中国畜牧兽医,2011,38(4)：101-104.
[196]徐鹏,杨桂连,刘孝刚,等.仔猪人工感染猪囊尾蚴后外周血中淋巴细胞亚群的动态变化研究[J].吉林农业大学学报,2007,29(4)：440-442.
[197]ZUCJERMANN F A, GASKINS H R. Distribution of CD4/CD8 double-positive T lymphocytes in mucosa-associated lymphoid tissues[J]. Immunology,1996,87:493-499.
[198]SUMMERFIELD A, RZIHA H J, SAALMULLER A. Functional characterization of porcine CD4+, CD8+ extrathymic T lymphocytes [J]. Cellular Immunology,1996,168:291-296.
[199]YANG H, OURA CA L, KIRKHANM, et al. preparation of monoclonal anti-porcine crh antibodies and preliminary characterization of porcine T lymphccytes[J]. Immunology,1996,88: 577-585.
[200]胡永明,赵明秋,陈立军,等.猪瘟病毒感染对猪外周血T淋巴细胞亚群及TNF-α和IFN-γ的影响[J].中国预防兽医学报,2011,33(2)：126-129.
[201]沈艳,华修国,崔立,等.猪链球菌2型两代表性分离株感染小型猪后对部分免疫指标的影响[J].畜牧兽医学报,2008,39(12)：1721-1730.
[202]张振斌,蒋宗勇,等.超早期断奶应激对仔猪T淋巴细胞亚群的影响[J].中国畜牧杂志,1999,35(3)：16-18.
[203]王珊,廖杰,于立方,等.流式细胞术快速植测T细胞亚群和NKT细胞亚群的免疫荧光变化[J].现代科学仪器,2010(3)：95-97.
[204]孙金福,史子学,郭焕成,等.猪瘟病毒感染猪外周血白细胞凋亡及CD4+和CD8+T淋巴细胞亚群的变化[J].中国兽医科学,2008,38(4)：342-345.
[205]李华,杨汉春,等.猪繁殖与呼吸综合征病毒感染仔猪淋巴细胞亚群的动态[J].中国免疫学杂志,2001,17(4)：208-211.
[206]顾有方.猪生殖-呼吸道综合征病毒感染猪淋巴细胞亚群的变化[J].国外兽医学：畜禽传染病,1998,18(3)：47-49.
[207]Williams NH. StahlyTS, Zimmerman DR. Effect of chronic immune system activation on the rate, efficiency and composition of growth and lysine needs of pigs fed from 6 to 27 kg[J]. J Anita Sci, 1997a,75:2463-2471.
[208]W illiams N H. Stahly T S. Zimmerman D R. Effect of chronic imm une system activation on body nitrogen retention, partial efficiency of lysine utilization and lysine needs of pigs[J]. J Anim Sci,1997b,75:2472-2480.
[209]Kim H M, Han S B, Oh G T, Kim Y H. Stimulation of humoral and cell mediated immunity by polysaccharide from mushroom Phellinus linteus. International Journal of Immunopharmacology, 1996,18:295-303.
[210]季敬璋,彭颖,吕建新.牛膝多糖体外诱导人T细胞表达IFN-γ和IL-4蛋白的机制探讨.中国免疫学杂志,2003,19(9)：611-613.
[211]彭西,崔赘,杨帆,等.日粮高硒对雏鸡外周血T淋巴细胞增殖功能和亚群的影响.畜牧兽医学报,2009,40(2)：256-26.
[212]张佩君.猪外周血单个核细胞和植物血凝素对猪淋转试验的影响[J].陕西教育学院学报,2007,23(1)：88-90,111.
[213]李瑶.基因芯片与功能基因组[M].化学工业出版社,2004.
[214]李瑶,陈菊祥.基因芯片的制备研究[J].第二军医大学学报.2000,21(009)：812-814.
[215]Auer H, Newsom DL, Kornacker K. Expression Profiling Using Affymetrix GeneChip Microarrays[J]. Methods Mol Biol.2009,509:35-46.
[216]Katagiri F, Glazebrook J. Overview of mRNA expression profiling using DNA microarrays[J]. Curr Protoc Mol Biol.2009, Chapter 22:Unit 22-24.
[217]Schena M, Shalon D, Heller R, et al. Parallel human genome analysis:microarray-based expression monitoring of 1000 genes[J]. Proc Natl Acad Sci USA,1996,93(20):10614-10619.
[218]Chitko-McKown CG, Fox JM, Miller LC, et al. Gene expression profiling of bovine macrophages in response to Escherichia coli 0157:H7 lipopolysaccharide[J]. Dev Comp Immunol,2004,28(6): 635-645.
[219]Dvorak CM, Hyland KA, Machado JG, et al. Gene discovery and expression profiling in porcine Peyer's patch [J]. Vet Immunol lmmunopathol,2005,105(3-4):301-315.
[210]Gerhold D, Lu M, Xu J, et al. Monitoring expression of genes involved in drug metabolism and toxicology using DNA microarrays[J]. Physiol Genomics.2001,5(4):161-170.
[211]Behr MA, Wilson MA, Gill WP, et al. Comparative genomics of BCG vaccines by whole-genome DNA microarray[J]. Science,1999,284(5419):1520-1523.
[212]Manger ID, Relman DA. How the host 'sees' pathogens:global gene expression responses to infection[J]. Curr Opin Immunol.2000,12(2):215-218.
[213]Klein RJ, Zeiss C, Chew EY, et al. Complement factor H polymorphism in age-related macular degeneration[J]. Science.2005,308(5720):385-389.
[214]Morimoto M, Zarlenga D, Beard H, et al. Ascaris suμm:cDNA microarray analysis of 4th stage larvae (L4) during self-cure from the intestine [J]. Exp Parasitol.2003,104(3-4):113-121.
[1]Taylor DJ. Actinobacillus pleuropneumoniae. In:Straw BE, D'Allaire S, Mengeling WL and Taylor DJ, editor. Diseases of swine.8th. Vol.26. Ames, Iowa, Iowa State University Press; 1999. pp:343-354.
[2]Wallgren P, Segal T, Morner AP, et al. Experimental infections with Actinobacillus pleuropneumoniae in pig-I. Comparison of five different parenteral antibiotic treaments[J]. J Vet Med B,1999,46:249-260.
[3]Fodor L, Reeve-Johnson L, Hodge A, et al. Efficacy evalution of the use of oral rilmicosin in pneumonic calves[J]. Immunol Immunopathol,2000,51:377-385.
[4]Liu JZ, Fung KF, Chen ZL, et al.Pharmacokinetics of Florfenticol in Healthy pigs annd pigs Experimentally Infected with Actinobacillus pleuropneumoniae[J]. Antimicrob Agents Ch,2003,47:820-823.
[5]杨旭夫,彭发泉.我国猪胸膜肺炎嗜血杆菌感染症的确定和诊断[J].畜牧兽医学报,1990,(03)：243-245.
[6]Hoflack G, D. Maes, B. Mateusen, et al. De Kruif. Efficacy of Tilmicosin Phosphate (Pulmotil Premix) in feed for the treatment of a clinical outbreak of Actinobacillus pleuropneumoniae infection in growing-finishing pigs[J]. J. Vet. Med. B,2001,48: 655-664.
[7]Blackall, P.2001. Pleuropneumonia in pigs. Agency for Food and Fibre Sciences. Deartment of Primary Industries, Queensland Government. Available: http://www.dpi.qld.gov.au/pigs/4463. html. Accessed Jan.13,2004.
[8]陈如明,谢鸣星,李云峰,等.鲁豫地区驻军猪传染性胸膜肺炎血清学调查[J].中国畜禽传染病,1990,(4)：38-40.
[9]邬捷,姜永康.四川猪嗜血杆菌胸膜肺炎血清学调查[J].动物检疫,1990(6)：21-22.
[10]汤万倾.我国猪传染性胸膜肺炎的危害及诊断防治研究[J].畜牧兽医科技信息.2004(04)：18-19。
[11]Jacques M. Surface polysaccharides and iron-uptake systems of Actinobacillus pleuropneumoniae [J]. Can J Vet Res,2004,68(2):81-85.
[12]张立昌.猪传染性胸膜肺炎研究进展[J].养猪,2001(1)：40-42。
[13]祝永凯,李文正,王成达,等.传染性胸膜肺炎的诊治[J].2010,31(1)：43.
[1]Baarsch M J, Foss D L, Murtaugh M P. Pathophysiologic correlates of acute porcine pleuropneumonia[J]. American Journal of Veterinary Research,2000,61(6):684-690.
[2]Taylor D J. Actinobacillus pleuropneumoniae. Diseases of swine[M]. Ames, Iowa: Iowa State University Press,1999:343-354.
[3]杨旭夫,彭发泉.我国猪胸膜肺炎嗜血杆菌感染症的确定和诊断[J].畜牧兽医学报,1990,21(3)：243-245.
[4]唐慧稳.猪传染性胸膜肺炎研究现状[J].畜牧兽医杂志,2003,22(4)：19-21.
[5]陈如明,谢鸣星,李云峰,等.鲁、豫地区驻军猪传染性胸膜肺炎血清学调查[J].中国畜禽传染病,1990(4)：38-40.
[6]邬捷,姜永康,曹国文,等.四川猪嗜血杆菌胸膜肺炎血清学调查[J].中国动物检疫,1990(6)：21-22.
[7]王俊东,刘宗平.兽医临床诊断学[M].北京：中国农业出版社,2004：221-245.
[8]王建华,伍淳操.抗菌肽和蛋氨酸锌对断奶仔猪部分血液常规指标的影响[J].贵州农业科学,2010,38(3)：127-130.
[9]王岩,王久伶.急性细菌性痢疾388例分析[J].西南国防医药,2009,19(12)：1206-1208.
[10]卿柳庭,袁宗辉.育成猪对猪链球菌的免疫应答[J].中国兽医学报,2002,(05)：47-50.
[11]陈琼,廖三赛,贺长青,等.抗仔猪断奶应激剂对断奶仔猪血清生化指标及血常规的影响[J].广西畜牧兽医,2008,24(3)：136-139.
[12]闫广运.不同肝脏疾病检测指标的特征性变化特点与分析[J].医学信息(下旬刊),2010,23(10)：115-116.
[13]马玉芳,林雪玲,池春梅,等.母猪喂服中药黄白痢散对哺乳仔猪血液的有关生理生化及免疫学参数的影响[J].福建农林大学学报(自然科学版),2008,37(3)：286-289.
[14]王蔚,陈代文,毛湘冰,等.饲粮中三聚氰胺的添加对育肥猪肾脏结构与功能的影响[J].动物营养学报,2011,23(9)：1584-1591.
[15]刘震,周树录,李炜如,等.缺氧缺血性脑病新生猪的肾脏损害[J].华西医科大学学报,1997,28(2)：201-203.
[16]黄少萍.重症肝炎并发低血糖23例临床分析[J].右江医学,2010,38(6)：749.
[17]韩惠瑛,马海利,张艳红,等.附红细胞体自然感染猪血液生化指标的检测[J].中国兽医杂志,2003,39(11)：20-22.
[18]邓发清.自然感染附红细胞体病猪血液生理生化指标的测定[J].中国兽医杂志,2007,43(11)：43-44.
[19]周定刚,郑维明,钟妮娜.催产时鳝卵磷酸酶活性的变化及其与排卵的关系[J].水生生物学报,1993,17(2)：145-150.
[20]圈华,李莉.青藏高原不同生长期牦牛15项指标的测定[J].动物医学进展,2011,32(8)：62-65.
[21]Wrenger S, Kahne T, Bohuon C, et al. Amino-terminal truncation of procalcitonin, a marker for systemic bacterial infections, by dipeptidyl peptidase IV (DP IV)[J]. FEBS Letters,2000,466(1): 155-159.
[22]Sankaran R T, Mattana J, Pollack S, et al. Laboratory Abnormalities in Patients With Bacterial Pneumonia[J]. Chest,1997,111(3):595-600.
[23]Muller, Becker, Kranzlin, et al. Disordered calcium homeostasis of sepsis:association with calcitonin precursors[J]. European Journal of Clinical Investigation,2000,30(9):823-831.
[24]刘慧琳,魏敏,刘涛.降钙素基因相关肽对肺泡Ⅱ型上皮细胞的保护作用[J].实用医学杂志,2011,27(4)：587-589.
[1]SIEGEL I, TIAN Liu-liu, NORBERT G. The red-cell immune system [J]. Lancet,1981,318(9): 556-559.
[2]郭峰,钱宝华,张乐之.现代红细胞免疫学[M].上海：第二军医大学出版社,2002,6.
[3]Siegel I, Gleicher N. Red cell immune adherence (RCIA):its application in cancer and autoimmune disease. Immunol Commun.1981; 10(4-5):433-449.
[4]郭峰,虞紫茜,赵中丰等.红细胞免疫功能的初步研究[J].中华医学杂志,1982,62(12)：715-716.
[5]胡金川.红细胞免疫及其临床应用研究进展[J].国际检验医学杂志,2008,29(7)：621-622.
[6]侯海锋,包永占,李茜,等.当归对鸡红细胞免疫功能的影响[J].中国兽医杂志,2009(4)：54-56.
[7]李晓卉,殷中琼.青刺果黄酮对鸡红细胞免疫及外周血淋巴细胞免疫功能的影响[J].中国兽医杂志,2009(3)：43-44.
[8]彭西,柏才敏,李敏,等.高硒对雏鸡红细胞免疫功能红细胞总数及血红蛋白含量的影响[J].中国兽医科学,2008,38(6)：535-539.
[9]崔恒敏,彭西,黎德兵,等.高锌对雏鹅红细胞免疫功能的影响[J].中国兽医学报,2004,24(6)：576-578.
[10]刘福堂,李艳飞,顾庆云.铝中毒对蛋鸡白细胞和红细胞免疫功能的影响[J].中国畜牧兽医,2007,34(10)：70-72.
[11]黎德兵,崔恒敏,赵翠燕.鸭疫里默杆菌病发病机理的研究Ⅳ.试验感染雏鸭红细胞免疫功能的变化[J].黑龙江畜牧兽医,2007(9)：61-62.
[12]周惠萍,郭定宗,贺建忠.硒中毒对猪红细胞免疫与自由基的影响[J].安徽农业科学,2010(5)：2376-2377,2380.
[13]李清艳,翟向和,吕建存,等.附红细胞体感染对猪红细胞免疫功能的影响[J].中国兽医科技,2004,34(4)：46-48.
[14]富艳玲,王德志,王晓旭,等.中药乳膏剂对乳房炎奶牛非特异性免疫和红细胞免疫功能的影响[J].中国兽医杂志,2008,44(7)：51-53.
[15]王丽,剡根强,王静梅.绵羊自然感染附红细胞体对绵羊红细胞的影响[J].石河子大学学报：自然科学版,2006,24(1)：108-111.
[16]屈雪琪,赵建增,梁智选,等.流式细胞术检测不同试验条件对猪T淋巴细胞亚群的影响[J].中国畜牧兽医,2011,38(4)：101-104.
[17]李铭,刘宝金.猪T淋巴细胞亚群的研究进展[J].国外兽医学：畜禽传染病,1998,18(4)：26-27.
[18]胡永明,赵明秋,陈立军,等.猪瘟病毒感染对猪外周血T淋巴细胞亚群及TNF-a和IFN-γ的影响[J].中国预防兽医学报,2011,33(2)：126-129.
[19]孙金福,史子学,郭焕成,等.猪瘟病毒感染猪外周血白细胞凋亡及CD4+和CD8+T淋巴细胞亚群的变化[J].中国兽医科学,2008,38(4)：342-345.
[20]李华,杨汉春.猪繁殖与呼吸综合征病毒感染仔猪淋巴细胞亚群的动态[J].中国免疫学杂志,2001,17(4)：208-211.
[21]顾有方.猪生殖-呼吸道综合征病毒感染猪淋巴细胞亚群的变化[J].国外兽医学：畜禽传染病,1998,18(3)：47-49.
[22]沈艳,华修国,崔立,等.猪链球菌2型两代表性分离株感染小型猪后对部分免疫指标的影响[J].畜牧兽医学报,2008,39(12)：1721-1730.
[23]徐鹏,杨桂连,刘孝刚,等.仔猪人工感染猪囊尾蚴后外周血中淋巴细胞亚群的动态变化研究[J].吉林农业大学学报,2007,29(4)：440-442.
[24]Williams NH. Stahly TS, Zimmerman DR. Effect of chronic immune system activation on the rate, efficiency and composition of growth and lysine needs of pigs fed from 6 to 27 kg[J]. J Anita Sci, 1997a,75:2463-2471.
[25]Williams NH. Stahly TS. Zimmerman DR. Effect of chronic immune system activation on body nitrogen retention, partial efficiency of lysine utilization and lysine needs of pigs[J]. J Anim Sci, 1997b,75:2472-2480.
[26]Kim HM, Han SB, Oh GT, et al. Stimulation of humoral and cell mediated immunity by polysaccharide from mushroom Phellinus linteus. International Journal of Immunopharmacology, 1996,18:295-303.
[27]季敬璋,彭颖,吕建新.牛膝多糖体外诱导人T细胞表达IFN-γ和IL-4蛋白的机制探讨.中国免疫学杂志,2003,19(9)：611-613.
[28]Selye H. A Syndrome produced by diverse nocuous agents[J]. J Neuropsychiatry Clin Neurosci, 1998,10(2):230-231.
[29]Mader T L. Environmental stress in confined beef cattle [J]. J Anim Sci,2003,81(14):110-119.
[30]Eckersall PD, Saini PK, McComb C. The acute phase response of acid soluble glycoprotein, a1-acid glycoprotein, ceruloplasmin, haptoglobin and C-reactive protein, in the pig [J]. Vet Immunol Immunopathol,1996,51(3-4):377-385.
[31]Heegaard PMH, Klausen J, Nielsen JP, et al. The porcine acute phase response to infection with Actinobacillus pleuropneumoniae. Haptoglobin, C-reactive protein, major acute phase protein and serum amyloid A protein are sensitive indicators of infection[J]. Comp Biochem Physiol B Biochem Mol Biol,1998,119(2):365-373.
[32]Lauritzen B, Lykkesfeldt J, Skaanild MT, et al. Putative biomarkers for evaluating antibiotic treatment:an experimental model of porcine Actinobacillus pleuropneumoniae infection [J]. Res Vet Sci,2003,74(3):261-270.
[33]Hulten C, Johansson E, Fossum C, et al. Interleukin-6, serum amyloid A and haptoglobin as marker of treaqtment efficacy in pigs experimentally infected with Actinobacillus pleuropneumoniae [J]. Vet Microbiol,2003,95(1-2):75-89.
[34]Fossum C, Wattrang E, Fuxler L, et al. Evaluation of various cytokines (IL-6, IFN-a, IFN-g, TNF-a) as markers for acute bacterial infection in swine-a possible role for serum innterleukin-6[J]. Veterinary Immunology and Immunopathology,1998,64(2):161-172.
[35]彭西.日粮硒对雏鸡免疫功能影响的机理研究[D].四川农业大学博士论文,2009.
[36]彭西,崔赞,杨帆,等.日粮高硒对雏鸡外周血T淋巴细胞增殖功能和亚群的影响[J].畜牧兽医学报,2009,40(2)：256-260.
[37]周武,汪德清.红细胞功能检测方法的研究进展[J].中国输血杂志,2010,23(8)：648-650.
[38]薛拥志,王爱华,利凯.红细胞免疫研究进展[J].安徽农业科学,2007,35(12)：3558-3560,3664.
[39]刘景田,党小军.红细胞作为免疫细胞的事实及意义[J].深圳中西医结合杂志,2002,12(1)：11.
[40]王彩虹.红细胞免疫调控研究进展[J].中国兽医杂志,2002,38(2)：28-31.
[41]KIDD M T. Zinc metabolism with special reference to its role in immunity[J]. World Poult Sci J, 1996,52(2)：309-324.
[42]盛永杰,陈维多.硒对奶牛红细胞谷胱甘肽过氧化物酶活力的浓度依赖性研究[J].黑龙江畜牧兽医,2001,44(8)：8-9.
[43]温立斌,何孔旺,杨汉春,等.类圆环病毒因子P1感染对猪外周血T淋巴细胞含量和增殖活性的影响[J].华北农学报,2009,24(B08)：1-4.
[44]张佩君.猪外周血单个核细胞和植物血凝素对猪淋转试验的影响[J].陕西教育学院学报,2007,23(1)：88-90.
[45]杜念兴.兽医免疫学[M].北京：中国农业出版社,2002.
[46]Crawley A, Wilkie B N. Porcine Ig isotypes:function and molecular characteristics[J]. Vaccine, 2003,21(21-22):2911-2922.
[47]Brandtzaeg P. Molecular and cellular aspects of the secretory immunoglobulin system[J]. APMIS, 1995,103(1):1-19.
[48]Macpherson A J, Mcco YKD, Johansen FE, et al. The immune geography of IgA induction and function [J]. Mucosal Immunology,2008,1:11-22.
[49]周建.支气管肺炎患儿治疗前后血清IGF-Ⅱ、Hs-CRP和血液SIgA水平检测的临床意义[J].淮海医药,2011；29(4)：311-312.
[50]赵武述.免疫平衡研究及其临床意义[M].北京：科学出版社,2005：90.
[51]Malle E, De Beer F C. Human serum amyloid A (SAA) protein:a prominent acute-phase reactant for clinical practice [J]. Eur J Clin In-vest,1996,26(6):427-435.
[52]Yip T, Chan J, Cho W, et al. Protein chip array profiling analysis in patients with severe acute respirator syndrome identified serum amyloid A protein as a biomarker potentially useful in monitoring the extent of pneumonia[J]. Clin Chem,2005,51(1):47-55.
[53]Kokubun M, Imafuku Y, Okada M, et al. Serum amyloid A (SAA) concentration varies among rheumatoid arthritis patients estimated by SAA/CRPratio [J]. Clin ChimicaActa,2005,360 (1-2):97-102.
[54]Volanakis J E. Human C- reactive protein: expression, structure, and function[J]. Mol Immunol, 2001,38(2/3):189-197.
[55]GB Toews, Cytokines and the lung[J]. Eur. Respir J,2001,18(34):3-17.
[56]Duh EJ, Maury WJ, Folks TM, et al. Tumor necrosis factor alpha activates human immunodeficiency virus type 1 through induction of nuclear factor binding to the NF-kappa B sites in the long terminal repeat[J]. Proc Natl Acad Sci,1989,86 (15):5974-5978.
[57]Lowenthal JW, Ballard DW, Bohnlein E, et al. Tumor necrosis factor alpha induces proteins that bind specifically to kappa B-like enhancer elements and regulate interleukin 2 receptor alpha-chain gene expression in primary human T lymphocytes[J]. Proc Natl Acad Sci,1989,86(7):2331-2335.
[58]Bussolino F, G Camussi, C Baglioni. Synthesis and release of platelet-activating factor by human vascular endothelial cells treated with tumor necrosis factor or interleukin 1 alpha [J]. J Biol Chem, 1988,263(24):11856-11861.
[59]Libby P, JM Ordovas, KR Auger, et al. Endotoxin and tumor necrosis factor induce interleukin-1 gene expression in adult human vascular endothelial cells[J]. Am J Pathol,1986,124(2):179-185.
[60]PP Nawroth, I Bank, D Handley, et al. Tumor necrosis factor/cachectin interacts with endothelial cell receptors to induce release of interleukin 1[J]. J Exp Med,1986,163(6):1363-1375.
[61]Molnar L, Berki T, Hussain A, et al. Detection of TNFalpha expression in the bone marrow and determination of TNFalpha production of peripheral blood mononuclear cells in myelodysplastic syndrome[J]. Pathol Oncol Res,2000,6(l):18-23.
[62]Maier SF, Watkins LR. Cytokines for psychologists:implications of bidirectional immune-to-brain communication for understanding behavior, mood, and cognition[J]. Psychol Rev 1998,105: 83-107.
[63]Dinarello C A. Biologic basis for interleukin-1 in disease[J]. Blood,1996,87(6):2095-2147.
[64]Marz W, Wieland H. HMG-CoA reeducates inhibition:antiinflammatory effects beyond lipid lowering[J]. Herz,2000,25(2):117-125.
[65]王光辉,陈传兵,王献武.支气管肺炎患儿治疗前后血清IGF、IL-I、IL-6、IL-8和hs-CRP检测的临床意义[J].放射免疫学杂志,2009,22(4)：337-338.
[66]陈侃侃.肺炎支原体肺炎患儿血清hs-CRP、IL-6和IL-8检测的临床意义[J].放射免疫学杂志,2011,24(3)：266-267.
[67]Dooms H, Kahn E, Knoechel B, et al. IL-2 induces a competitive survival advantage in T lymphocytes[J]. J Immunol,2004,172(10):5973-5979.
[68]李爱华,曹祥山.IL-2与CD4+CD5+调节性T细胞的相关研究进展[J].医学综述,2007,13(9)：652-653.
[69]Trojanowska M. Role of PDGF in fibrotic diseases and systemic sclerosis[J]. Rheumatology,2008, 47(5):2-4.
[70]谢元林,藏雄益.国外医学.生理、病理科学与临床分册.1998,3：283-285.
[71]Gulumian M, Borm P J, Vallyathan V, et al. Mechanistically identified suitable biomarkers of exposure, effect, and susceptibility for silicosis and coal-worker's pneumoconiosis:a comprehensive review [J]. J Toxicol Environ Health B Crit Rev,2006,9(5):357-395.
[72]何燕,邓国忠,高天.肺纤维化细胞因子机制研究进展[J].中华创伤杂志,2006,6(22)：477-480.
[73]姚武,王治明,王绵珍等.煤工尘肺患者血清细胞因子及氧化损伤水平研究[J].四川大学学报(医学版),2005,36(4)：510-512.
[74]Santana A, Saxena B, Nancy A, et al. Increased expression of TGF-B1 in bleomycin-induced pulmonary fibrosis[J]. Am J Respir Cell Mol Biol,1995,13(1):34-44.
[75]Nagahori T, Dohi M, Matsumoto K, et al. Interferon-γ Upregulates the c-Met/Hepatocyte Growth Factor Receptor Expression in Alveolar Epithelial Cells[J]. Am J Respir Cell Mol Biol,1999,21(4): 490-497.
[76]Bajwa EK, Ayas NT, Schulzer M, et al. Interferon-gammalb therapy in idiopathic pulmonary fibrosis:a metaanalysis. Chest,2005,128(1):203-206.
[1]孙存普,张建中,段绍瑾.自由基生物学导论[M].合肥：中国科学技术大学出版社,1999.
[2]赵祥文.自由基与严重感染[J].临床儿科杂志,1993,11(3)：147-149.
[3]Imai Y, Kuba K, Neely GG, et al. Identification of oxidative stress and Toll-like receptor 4 signaling as a key pathway of acute lung injury[J]. Cell,2008,133(2): 235-249.
[4]杨旭夫,彭发泉.我国猪胸膜肺炎嗜血杆菌感染症的确定和诊断[J].畜牧兽医学报,1990,(03)：243-245.
[5]陈如明,谢鸣星,李云峰,等.鲁豫地区驻军猪传染性胸膜肺炎血清学调查[J].中国畜禽传染病,1990,(4)：38-40.
[6]邬捷,姜永康.四川猪嗜血杆菌胸膜肺炎血清学调查[J].动物检疫,1990(6)：21-22.
[7]Vander Vliet A, Cross CE. Oxidants, nitrosants, and the lung[J]. Am J Med,2000, 109(5):398-421.
[8]Vukic Z. Gluteal gunshot wounds [J]. Mill Med,2000,165(3):237-239.
[9]Ilic N, Petricevic A, Tanfara S, et al. War injuries to the chest. Acta Chir Hung,1999, 38:43-47.
[10]李清艳,翟向和,钟秀会,附红细胞体自然感染病猪血浆NO、SOD和MDA的变化[J].动物医学进展,2005,26(2)：92-94.
[11]毕晋明,郑姗姗,张敏红,等.日粮中添加不同浓度吡啶甲酸铬对生长肥育猪氧化作用的影响[J].动物营养学报,2008,20(5)：561-566.
[12]赵晶,康世良.健康仔猪口服亚硒酸钠后血液硒浓度与抗氧化系统动态变化规律的研究[J].畜牧兽医学报,2003,34(6)：554-557.
[13]Abu-khader AA, Biho YY. Exposure of human meutrophils to oxygen redicals causes loss of deformability. lipid peroxidation, protein degradation, respiratory burst activation and loss of migration[J]. Clin Hemorheol Microcirculat,2002,27(1): 57-66.
[14]Alverdy A M. Efects of glutamine-supplemented diets on immunology of the gut[J]. Parenter Enteral Nutr,1990,14:109-113.
[15]李敏,崔伟,彭西,等.高铜对雏鸡脑组织抗氧化酶活性的影响[J].畜牧兽医学报,2010,41(2)：220-22.
[16]王金胜,郭春绒,张映.农业生物化学技术[M].太原：山西科学技术出版社,1997：105-110.
[17]莫简.自由基在生物体系中的作用：普通中间介质或非特异性调节剂[J].自由基生命科学进展,1999(7)：7-10.
[18]李华强,史源,潘捷,等.内源性一氧化氮在感染性肺炎病理过程中的作用[J].第三军医大学学报,1999,21(3)：206-208.
[19]龙建纲,王学敏,高宏翔,等.丙二醛对大鼠肝线粒体呼吸功能及相关脱氢酶活性影响[J].第二军医大学学报,2005,26(10)：1131-1135.
[1]Hu J, He X. Enhanced quantile normalization of microarray data to reduce loss of information in gene expression profiles[J], Biometrics,2007,63(1):50-59.
[2]Xia X, McClelland M, Wang Y. WebArray:an online platform for microarray data analysis[J], BMC bioinformatics,2005,6(1):306.
[3]Romualdi C, Vitulo N, Favero M D, et al. MIDAW:a web tool for statistical analysis of microarray data[J], Nucleic acids research,2005,33(Web Server Issue):W644.
[4]Saeed AI, Sharov V, White J, et al. TM4:a free, open-source system for microarray data management and analysis[J]. Biotechniques.2003,34(2):374.
[5]Troyanskaya O3 Cantor M, Sherlock G, et al. Missing value estimation methods for DNA microarrays[J], Bioinformatics,2001,17(6):520.
[6]Pirooznia M, Nagarajan V, Deng Y. GeneVenn-A web application for comparing gene lists using Venn diagrams[J]. Bioinformation.2007,1(10):420.
[7]Lee HK, Braynen W, Keshav K, et al. ErmineJ:tool for functional analysis of gene expression data sets[J]. BMC Bioinformatics.2005,6(1):269.
[8]Mootha VK, Lindgren CM, Eriksson KF, et al. PCG-1a-responsive genes involved in oxidative phosphorylation are coordinately downregulated in human diabetes[J]. Nature genetics.2003, 34(3):267-273.
[9]Bild A, Febbo PG. Application of a priori established gene sets to discover biologically important differential expression in microarray data[J]. Proceedings of the National Academy of Sciences. 2005,102(43):15278.
[10]顾祖光.基因芯片数据分析中的基因集合分析技术[D].河北农业科学,2008,12(008)：169-170.
[11]Dennis Jr G, Sherman BT, Hosack DA, et al. DAVID:database for annotation, visualization, and integrated discovery[J]. Genome Biol,2003,4(5):3.
[12]Aoki-Kinoshita K F, Kanehisa M. Gene annotation and pathway mapping in KEGG[J]. Methods in MOLECULAR BIOLOGY-CLIFTON THEN TOTOWA,2007,396:71.
[13]Subramanian A, Tamayo P, Mootha VK, et al. Gene set enrichment analysis:a knowledge-based approach for interpreting genome-wide expression profiles[J]. Proceedings of the National Academy of Sciences.2005,102(43):15545.
[14]Erkens T, Van Poucke M, Vandesompele J, et al. Development of a new set of reference genes for normalization of real-time RT-PCR data of porcine backfat and longissimus dorsi muscle, and evaluation with PPARGC1A[J]. BMC Biotechnol,2006,6:41-52.
[15]Simmons PT, Portier CJ. Toxicogenomics:the new frontier in risk analysis[J], Carcinogenesis, 2002,23(6):903.
[16]Geller SC, Gregg JP, Hagerman P, et al. Transformation and normalization of oligonucleotide microarray data[J]. Bioinformatics.2003,19(14):1817-1823.
[17]Quackenbush J. Microarray data normalization and transformation[J]. Nat Genet.2002,32 Suppl: 496-501.
[18]邱浪波.基因芯片表达数据分析相关问题研究[D].湖南长沙,国防科学技术大学,2007.
[19]王永煜,张幼怡.基因芯片数据分析与处理[J].生物化学与生物物理进展.2003,30(002)：321-323.
[20]伍亚舟,张彦琦,黄明辉,等.基因芯片表达数据的标准化策略研究[J].第三军医大学学报.2004,26(007)：594-597.
[21]谈效俊,张永新,钱敏,等.芯片数据标准化方法比较研究[J].生物化学与生物物理进展，2007,34(006)：625-633.
[22]Oba S, Sato MA, Takemasa I, et al. A Bayesian missing value estimation method for gene expression profile data[J]. Bioinformatics.2003,19(16):2088-2096.
[23]Ouyang M, Welsh WJ, Georgopoulos P. Gaussian mixture clustering and imputation of microarray data[J]. Bioinformatics.2004,20(6):917-923.
[24]Kim H, Golub GH, Park H. Missing value estimation for DNA microarray gene expression data: local least squares imputation[J]. Bioinformatics.2005,21(2):187-198.
[25]Yoon D, Lee EK, Park T. Robust imputation method for missing values in microarray data[J]. BMC Bioinformatics.2007,8 Suppl 2:S6.
[26]Kerr MK. Design considerations for efficient and effective microarray studies[J], Biometrics,2003, 59(4):822-828.
[27]Tanaka TS, Jaradat SA, Lim MK, et al. Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray[J]. Proceedings of the National Academy of Sciences of the United States of America.2000,97(16):9127.
[28]Yang IV, Chen E, Hasseman JP, et al. Within the fold:assessing differential expression measures and reproducibility in microarray assays[J]. Genome Biol.2002,3(11):1-0062.0012.
[29]Tusher VG, Tibshirani R, Chu G. Significance analysis of microarrays applied to the ionizing radiation response[J]. Proc Natl Acad Sci USA.2001,98(9):5116-5121.
[30]Wright GW, Simon RM. A random variance model for detection of differential gene expression in small microarray experiments [J]. Bioinformatics.2003,19(18):2448-2455.
[31]Baldi P, Long AD. A Bayesian framework for the analysis of microarray expression data: regularized t-test and statistical inferences of gene changes[J]. Bioinformatics.2001,17(6): 509-519.
[32]Long AD, Mangalam HJ, Chan BY, et al. Improved statistical inference from DNA microarray data using analysis of variance and a Bayesian statistical framework. Analysis of global gene expression in Escherichia coli K12[J]. J Biol Chem.2001,276(23):19937-19944.
[33]孙如平.结合基因组调控信息的基因芯片数据综合分析[D].复旦大学；2007.
[34]Heyer LJ, Kruglyak S, Yooseph S. Exploring expression data: identification and analysis of coexpressed genes [J]. Genome Res.1999,9(11):1106-1115.
[35]Tavazoie S, Hughes JD, Campbell MJ, et al. Systematic determination of genetic network architecture[J]. Nat Genet.1999,22(3):281-285.
[36]Tamayo P, Slonim D, Mesirov J, et al. Interpreting patterns of gene expression with self-organizing maps:methods and application to hematopoietic differentiation [J]. Proc Natl Acad Sci USA.1999, 96(6):2907-2912.
[37]Breiman L. Arcing classifiers[J]. Annals of statistics.1998,26(3):801-824.
[38]Seok J, Kaushal A, Davis RW, et al. Knowledge-based analysis of microarrays for the discovery of transcriptional regulation relationships[J]. BMC Bioinformatics.11 Suppl 1:S8.
[39]Winters-Hilt S, Yelundur A, McChesney C, et al. Support vector machine implementations for classification & clustering[J]. BMC Bioinformatics.2006,7 Suppl 2:S4.
[40]蔡立军.基因分类及基因表达数据分析方法的研究[D].湖南大学；2007.
[41]李明洲.脂肪型和瘦肉型猪肌肉生长和脂肪沉积相关基因的差异表达分析和分子网络构建[D].四川农业大学,2008.
[42]Rhee S Y, Wood V, Dolinski K, et al. Use and misuse of the gene ontology annotations[J]. Nature Reviews Genetics,2008,9(7):509-515.
[43]Hong E L, Balakrishnan R, Dong Q, et al. Gene Ontology annotations at SGD:new data sources and annotation methods[J]. Nucleic acids research,2008,36(Database issue):D577.
[44]Camon E, Barrell D, Lee V, et al. The Gene Ontology Annotation (GOA) Database-an integrated resource of GO annotations to the UniProt Knowledgebase[J]. In Silico Biology,2004,4(1):5-6.
[45]Ogata H, Goto S, Fujibuchi W, et al. Computation with the KEGG pathway database[J]. Biosystems,1998,47(1-2):119-128.
[46]Arakawa K, Kono N, Yamada Y, et al. KEGG-based pathway visualization tool for complex omics data[J]. In Silico Biology,2005,5(4):419-423.
[47]Paterlini M, Zakharenko SS, Lai WS, et al. Transcriptional and behavioral interaction between 22q11.2 orthologs modulates schizophrenia-related phenotypes in mice[J]. Nature neuroscience. 2005,8(11):1586-1594.
[48]方焯.基于生物知识的生物芯片表达谱数据分析研究[D].湖北武汉,华中科技大学,2006.
[49]Dopazo J. Functional interpretation of microarray experiments[J]. OMICS:A Journal of Integrative Biology,2006,10(3):398-410.
[50]Shaw MH, Reimer T, Kim YG, et al. NOD-like receptors (NLRs):bona fide intracellular microbial sensors [J]. Curr Opin Immunol,2008,20:377-82.
[51]Kanneganti TD, Lamkanfi M, Nunez G. Intracellular NOD-like receptors in host defense and disease[J]. Immunity,2007,27:549-59.
[52]Franchi L, Warner N, Viani K, et al. Function of Nod-like receptors in microbial recognition and host defense[J]. Immunol Rev,2009,227:106-28.
[53]Tattoli I, Travassos LH, Carneiro LA, et al. The Nodosome:Nod1 and Nod2 control bacterial infections and inflammation[J]. Semin Immunopathol 29:289-301.
[54]Reardon C, Mak TW. cIAP proteins:keystones in NOD receptor signal transduction[J]. Immunity 30:755-6.
[55]Fritz JH, Ferrero RL, Philpott DJ, et al. Nod-like proteins in immunity, inflammation and disease[J]. Nat Immunol,2006,7:1250-7.
[56]Rietdijk ST, Burwell T, Bertin J, et al. Sensing intracellular pathogens-NOD-like receptors[J]. Curr Opin Pharmacol,2008,2008,8:261-6.
[57]Franchi L, Eigenbrod T, Munoz-Planillo R, et al. The inflammasome:a caspase-1-activation platform that regulates immune responses and disease pathogenesis[J]. Nat Immunol,2009, 10:241-7.
[58]Martinon F, Gaide O, Petrilli V, et al. NALP inflammasomes:a central role in innate immunity[J]. Semin Immunopathol,2007,29:213-29.
[59]Martinon F, Tschopp J. Inflammatory caspases:linking an intracellular innate immune system to autoinflammatory diseases[J]. Cell,2004,117:561-74.
[60]Benko S, Philpott DJ, Girardin SE. The microbial and danger signals that activate Nod-like receptors[J]. Cytokine,2008,43:368-73.
[61]Kawakami T, Galli SJ. Regulation of mast-cell and basophil function and survival by IgE[J]. Nat Rev Immunol,2002,2:773-86.
[62]Siraganian RP. Mast cell signal transduction from the high-affinity IgE receptor[J]. Curr Opin Immunol,2003,15:639-46.
[63]Turner H, Kinet JP. Signalling through the high-affinity IgE receptor Fc epsilonRI[J]. Nature,1999, 402:B24-30.
[64]Gilfillan AM, Tkaczyk C. Integrated signalling pathways for mast-cell activation[J]. Nat Rev Immunol,2006,6:218-30.
[65]Nadler MJ, Matthews SA, Turner H, et al. Signal transduction by the high-affinity immunoglobulin E receptor Fc epsilon RI:coupling form to function[J]. Adv Immunol,2000,76:325-55.
[66]Steffen B, Muller-Tidow C, Schwable J, et al. The molecular pathogenesis of acute myeloid leukemia[J]. Crit Rev Oncol Hematol,2005,56:195-221.
[67]Tenen DG. Disruption of differentiation in human cancer: AML shows the way[J]. Nat Rev Cancer, 2003,3:89-101.
[68]Martelli AM, Nyakern M, Tabellini G, et al. Phosphoinositide 3-kinase/Akt signaling pathway and its therapeutical implications for human acute myeloid leukemia[J]. Leukemia,2006,20:911-28.
[69]Lennartsson J, Jelacic T, Linnekin D, et al. Normal and oncogenic forms of the receptor tyrosine kinase kit[J]. Stem Cells,2005,23:16-43.
[70]Lorsbach RB, Downing JR. The role of the AML1 transcription factor in leukemogenesis[J]. Int J Hematol,2001,74:258-65.
[71]Mizuki M, Schwable J, Steur C, et al. Suppression of myeloid transcription factors and induction of STAT response genes by AML-specific Flt3 mutations[J]. Blood,2003,101:3164-73.
[72]Nebreda AR, Ferby I. Regulation of the meiotic cell cycle in oocytes[J]. Curr Opin Cell Biol,2000, 12:666-75.
[73]Andersen CB, Sakaue H, Nedachi T, et al. Protein kinase B/Akt is essential for the insulin-but not progesterone-stimulated resumption of meiosis in Xenopus oocytes[J]. Biochem J,2003, 369:227-38.
[74]Abrieu A, Doree M, Fisher D. The interplay between cyclin-B-Cdc2 kinase (MPF) and MAP kinase during maturation of oocytes[J]. J Cell Sci,2001,114:257-67.
[75]Ghayee HK, Auchus RJ. Basic concepts and recent developments in human steroid hormone biosynthesis[J]. Rev Endocr Metab Disord,2007,8:289-300.
[76]Holmes MC, Seck1 JR. The role of 1 lbeta-hydroxysteroid dehydrogenases in the brain[J]. Mol Cell Endocrinol,2006,248:9-14.
[77]Thomas JL, Duax WL, Addlagatta A, et al. Structure/function relationships responsible for coenzyme specificity and the isomerase activity of human type 13 beta-hydroxysteroid dehydrogenase/isomerase[J]. J Biol Chem,2003,278:35483-90.
[78]Tomlinson JW, Stewart PM. Cortisol metabolism and the role of 1 lbeta-hydroxysteroid dehydrogenase[J]. Best Pract Res Clin Endocrinol Metab,2001,15:61-78.
[79]Gual P, Le Marchand-Brustel Y, Tanti JF. Positive and negative regulation of insulin signaling through IRS-1 phosphorylation[J]. Biochimie,2005,87:99-109.
[80]Kaneto H, Matsuoka TA, Nakatani Y, et al. Oxidative stress, ER stress, and the JNK pathway in type 2 diabetes[J]. J Mol Med,2005,83:429-39.
[81]Sakai K, Matsumoto K, Nishikawa T, et al. Mitochondrial reactive oxygen species reduce insulin secretion by pancreatic beta-cells[J]. Biochem Biophys Res Commun,2003,300:216-22.
[82]Robertson RP. Chronic oxidative stress as a central mechanism for glucose toxicity in pancreatic islet beta cells in diabetes[J]. J Biol Chem,2004,279:42351-4.
[83]Markus C. Wahl, Cindy L. Will, Reinhard Lu" hrmann. The spliceosome:design principles of a dynamic RNP machine[J]. Cell,136:701-18.
[84]Morris DP, Greenleaf AL. The splicing factor, Prp40, binds the phosphorylated carboxyl-terminal domain of RNA polymerase II[J]. J Biol Chem,2000,275:39935-43.
[85]Chen YI, Moore RE, Ge HY, et al. Proteomic analysis of in vivo-assembled pre-mRNA splicing complexes expands the catalog of participating factors[J]. Nucleic Acids Res,2007,35:3928-44.
[86]Ritchie DB, Schellenberg MJ, Macmillan AM. Spliceosome structure:Piece by piece[J]. Biochim BiophysActa,2009,1789:624-633.
[87]Levy C, Khaled M, Fisher DE. MITF:master regulator of melanocyte development and melanoma oncogene[J]. Trends Mol Med,2006,12:406-14.
[88]Tsao H, Goel V, Wu H, et al. Genetic interaction between NRAS and BRAF mutations and PTEN/MMAC1 inactivation in melanoma[J]. J Invest Dermatol,2004,122:337-41.
[89]Gruss C, Herlyn M. Role of cadherins and matrixins in melanoma[J]. Curr Opin Oncol,2001, 13:117-23.
[90]Lazar-Molnar E, Hegyesi H, Toth S,et al. Autocrine and paracrine regulation by cytokines and growth factors in melanoma[J]. Cytokine,2000,12:547-54.
[91]Liu T, Clark RK, McDonnell PC, et al. Tumor necrosis factor-alpha expression in ischemic neurons[J]. Stroke,1994,25:1481-8.
[92]Goukassian DA, Qin G, Dolan C, et al. Tumor necrosis factor-alpha receptor p75 is required in ischemia-induced neovascularization[J]. Circulation,2007,115:752-62.
[93]Yoshida S, Ono M, Shono T, Izumi H, et al. Involvement of interleukin-8, vascular endothelial growth factor, and basic fibroblast growth factor in tumor necrosis factor alpha-dependent angiogenesis[J]. Mol Cell Biol,1997,17:4015-23.
[94]Limb GA, Chignell AH, Green W, et al. Distribution of TNF alpha and its reactive vascular adhesion molecules in fibrovascular membranes of proliferative diabetic retinopathy[J]. Br J Ophthalmol,1996,80:168-73.
[95]NM Cirino, JR Panuska, A Villani, et al. Restricted replication of respiratory syncytial virus in human alveolar macrophages[J]. J Gen Virol,1993,74 (8):1527-1537.
[96]MT Heise, HWT Virgin. The T-cell-independent role of gamma interferon and tumor necrosis factor alpha in macrophage activation during murine cytomegalovirus and herpes simplex virus infections[J]. J Virol,1995,69(2):904-909.
[97]GH Wong, DV Goeddel. Tumour necrosis factors alpha and beta inhibit virus replication and synergize with interferons[J]. Nature,1986,323(6091):819-822.
[98]GB Toews. Cytokines and the lung[J]. Eur Respir J Suppl,2001,34:3-17.
[99]Duh EJ, WJ Maury, TM Folks, et al. Tumor necrosis factor alpha activates human immunodeficiency virus type 1 through induction of nuclear factor binding to the NF-kappa B sites in the long terminal repeat[J]. Proc Natl Acad Sci USA,1989,86:5974-5978.
[100]Kronke M, S Schutze, P Scheurich, et al. TNF signal transduction and TNF-responsive genes[J]. Immunol Ser,1992,56:189-216.
[101]Lowenthal JW, DW Ballard, E Bohnlein, et al. Tumor necrosis factor alpha induces proteins that bind specifically to kappa B-like enhancer elements and regulate interleukin 2 receptor alpha-chain gene expression in primary human T lymphocytes[J]. Proc Natl Acad Sci USA,1989, 86:2331-2335.
[102]Osborn L, S Kunkel, GJ Nabel. Tumor necrosis factor alpha and interleukin 1 stimulate the human immunodeficiency virus enhancer by activation of the nuclear factor kappa B[J]. Proc Natl Acad Sci USA,1989,86:2336-2340.
[103]Schutze S, K Potthoff, T Machleidt, et al.TNF activates NF-kappa B by phosphatidylcholine-specific phospholipase C-induced "acidic" sphingomyelin breakdown[J]. Cell, 1992,71:765-776.
[104]Bussolino F, G Camussi, C Baglioni. Synthesis and release of platelet-activating factor by human vascular endothelial cells treated with tumor necrosis factor or interleukin 1 alpha[J]. J Biol Chem, 1988,263:11856-11861.
[105]Libby P, JM Ordovas, KR Auger, et al. Endotoxin and tumor necrosis factor induce interleukin-1 gene expression in adult human vascular endothelial cells[J]. Am J Pathol,1986,124:179-185.
[106]Nawroth PP, I Bank, D Handley, et al.Tumor necrosis factor/cachectin interacts with endothelial cell receptors to induce release of interleukin 1[J]. J Exp Med,1986,163:1363-1375.
[107]Pober JS, M Gimbrone, LA Lapierre, et al. Overlapping patterns of activation of human endothelial cells by interleukin 1, tumor necrosis factor, and immuneinterferon[J]. J Immunol, 1986,137:1893-1896.
[108]Wajant H, Pfizenmaier K, Scheurich P. Tumor necrosis factor signaling[J]. Cell Death Differ, 2003,10:45-65.
[109]Molnar L, Berki T, Hussain A, et al. Detection of TNF alpha expression in the bone marrow and determination of TNFalpha production of peripheral blood mononuclear cells in myelodysplastic syndrome[J]. Pathol Oncol Res,2000,6:18-23.
[110]Chrysant SG. The path physiologic role of the brain Renin-Angiotensin system in stroke protection:clinical implications[J]. J Clin Hypertens(Greenwich),2007,9(6):454-459.
[111]Weiner OD. Regulation of cell polarity during eukaryotic chemotaxis:the chemotactic compass. Curr Opin Cell Biol,2002,14:196-202.
[112]Vanhaesebroeck B, Jones GE, Allen WE, et al. Distinct PI(3) Ks mediate mitogenic signalling and cell migration in macrophages. Nat Cell Biol,1999,1:69-71.
[113]Keqiang Chen, Pablo Iribarren, Wanghua Gong, et al. The Essential Role of Phosphoinositide 3-Kinases(PI3Ks)in Regulating Pro-Inflammatory Responses and the Progression of Cancer[J]. Cellular & Molecular Immunology,2005,2(4):241-252.
[114]Schmidt R, Redecke V, Breitfeld Y, et al. EMMPRIN (CD 147)is a central activator of extracellular matrix degradation by Chlamydia pneumoniae-infected monocytes. Implications for plaque rupture[J]. Thromb Haemost,2006,95(1):151-158.
[115]L Stephens, C Ellson, P Hawkins. Roles of PI3Ks in leukocytes chemotaxis and phagocytosis, Curr. Opin[J]. Cell Biol,14 (2002):203-213.
[116]MP Wymann, M Zvelebil, M Laffargue. Phosphoinositide 3-kinase signalling-which way to target?[J]. Trends Pharmacol Sci,2003,24:366-376.
[117]T Sasaki, J Irie-Sasaki, RG Jones, et al. Function of PI3Ky in thymocyte development, T cell activation, and neutrophil migration[J]. Science,2000,287:1040-1046.
[118]E Hirsch, VL Katanaev, C Garlanda, et al.Central role for G protein-coupled phosphoinositide 3-kinase y in inflammation[J]. Science,2000,287:1049-1053.
[119]Z Li, H Jiang, W Xie, et al. Roles of PLC-β2 and -β3 and PI3Ky in chemoattractant- mediated signal transduction[J]. Science,2000,287:1046-1049.
[120]MA Crackower, GY Oudit, I Kozieradzki, et al. Regulation of myocardial contractibility and cell size by distinct PI3K-PTEN signaling pathways[J]. Cell,2002,:737-749.
[121]Brock C, Schaefer M, Reusch HP, et al. (Jan). Roles of G beta gamma in membrane recruitment and activation of p110 gamma/p101 phosphoinositide 3-kinase gamma[J]. J Cell Biol,2003,160, (1):89-99.
[122]"Entrez Gene:PIK3R5 phosphoinositide-3-kinase, regulatory subunit 5, p101". http://www. ncbi. nlm. nih. gov/sites/entrez?Db=gene&Cmd=ShowDetailView & TermToSearch=23533.
[123]Voigt P, Brock C, Nurnberg B, et al. Assigning functional domains within the p101 regulatory subunit of phosphoinositide 3-kinase gamma[J]. J Biol Chem,2005,280(6):5121-7.
[124]Bassi R, Heads R, Marber MS, Clark JE. Targeting p38-MAPK in the ischaemic heart:kill or cure?[J]. Curr Opin Pharmacol,2008,8:141-6.
[125]Irving EA, Bamford M. Role of mitogen- and stress-activated kinases in ischemic injury[J]. J Cereb Blood Flow Metab,2002,22:631-47.
[126]Nozaki K, Nishimura M, Hashimoto N. Mitogen-activated protein kinases and cerebral ischemia[J]. Mol NeuroBiol,2001,23:1-19.
[127]Ravingerova T, Barancik M, Strniskova M. Mitogen-activated protein kinases:a new therapeutic target in cardiac pathology[J]. Mol Cell Biochem,2003,247:127-38.
[128]Bodil Gesslein, Gisela Hakansson, Ronald Carpio, et al. Mitogen-activated protein kinases in the porcine retinal arteries and neuroretina following retinal ischemia-reperfusion[J]. Mol Vis,2010, 16:392-407.
[129]Lai EW, Toledo-Pereyra LH, Walsh J, et al. The role of MAP kinases in trauma and ischemia-reperfusion[J]. J Invest Surg,2004,17:45-53.
[130]Roux PP, Blenis J. ERK and p38 MAPK-activated protein kinases:a family of protein kinases with diverse biological functions[J]. Microbiol Mol Biol Rev,2004,68:320-44.
[131]Kubota Y, O'Grady P, Saito H, et al. Oncogenic Ras abrogates MEK SUMOylation that suppresses the ERK pathway and cell transformation[J]. Nat Cell Biol,2011,13(3):282-91.
[132]Daughaday WH, Rotwein P. Insulin-like growth factor Ⅰ and Ⅱ. Peptide, messenger ribonucleic acid and gene structures, serum, and tissue concentrations[J]. Endocr Rev,1989,10:68-91.
[133]Christoforidis A, Maniadaki I, Stanhope R. Growth hormone/insulin-like growth factor-1 axis during puberty[J]. Pediatr Endocrinol Rev,2005,3:5-10.
[134]Dunshea FR, Barneveld RJ. Colostrum protein isolate enhances gut development, growth performance and plasma IGF-I and II in neonatal pigs Asia Pac[J]. J Clin Nutr,12:40.
[135]Boutinaud M, Shand JH, Park MA, et al. A quantitative RT-PCR study of the mRNA expression profile of the IGF axis during mammary gland development[J]. J Mol Endocrinol,33:195-207.
[136]Marz W, Wieland H. HMG-CoA reeducates inhibition:antiinflammatory effects beyond lipid lowering[J]. Herz,2000,25(2):117-125.
[137]Nakanishi K, Yoshimoto T, Tsutsui H, et al. Interleukin-18 regulates both Thl and Th2 responses[J]. Annu Rev Immunol,2001,19:423-474.
[138]Wolff B, Burns AR, Middleton J, et al. Endothelial cell "memory" of inflammatory stimulation: human venular endothelial cells store interleukin 8 in Weibel-Palade bodies[J]. J Exp Med,188(9): 1757-62.
[139]Utgaard JO, Jahnsen FL, Bakka A, et al. Rapid secretion of prestored interleukin 8 from Weibel-Palade bodies of microvascular endothelial cells[J]. J Exp Med,188(9):1751-6.
[140]Hoshino T, Wiltrout RH, Young HA. IL-18 is a potent coinducer of IL-13 in NK and T cells:A new potential role for IL-18 in modulating the immune response[J]. J Immunol,1999,162: 5070-5077.
[141]Yoshimoto T, Tsutsui H, Tominaga K, et al. IL-18, although antiallergic when administered with IL-12, stimulates IL-4 and histamine release by basophils[J]. Proc Natl Acad Sci USA,1999,96: 13962-13966.
[142]Yoshimoto T, Mizutani H, Tsutsui H, et al. IL-18 induction of lgE: Dependence on CD4+ T cells, IL-4 and STAT6[J]. Nat Immunol,2000,1,132-137.
[143]Yoshimoto T, Min B, Sugimoto T, et al. Nonredundant roles for CD Id-restricted natural killer T cells and conventional CD4+ T cells in the induction of immunoglobulin E antibodies in response to interleukin 18 treatment of mice. J Exp Med,2003,197:997-1005.
[144]Carr MW, Roth SJ, Luther E, et al. Monocyte chemoattractant protein 1 acts as a T-lymphocyte chemoattractant[J]. Proc Natl Acad Sci USA,1994,91(9):3652-6.
[145]Xu LL, Warren MK, Rose WL, et al. Human recombinant monocyte chemotactic protein and other C-C chemokines bind and induce directional migration of dendritic cells in vitro[J]. J Leukoc Biol,1996,60 (3):365-71.
[146]Peelman LJ, P Chardon, M Nunes, et al. The BAT1 gene in the MHC encodes an evolutionarily conserved putative nuclear RNA helicase of the DEAD family[J]. Genomics 1995,26:210-218.
[147]Spies T, G Blanck, M Bresnahan, et al. A new cluster of genes within the human major histocompatibility complex[J]. Science,1989,243:214-217.
[148]Spies T, M Bresnahan, JL Strominger.Human major histocompatibility complex contains a minimum of 19 genes between the complement cluster and HLA-B[J]. Proc Natl Acad Sci USA, 1989,86:8955-8958.
[149]Von Bubnoff D, Novak N, Kraft S, et al. The central role of FcepsilonRI in allergy[J]. Clin Exp Dermatol,28(2):184-7.
[150]Gounni A, Lamkhioued B, Ochiai K, et al. High-affinity IgE receptor on eosinophils is involved in defence against parasites[J]. Nature,367 (6459):183-6.
[151]Kisseleva T, Bhattacharya S, Braunstein, J, et al. Signaling through the JAK/STAT pathway, recent advances and future changes[J]. Gene,2002,285(1-2),1-24.
[152]Jakob Hedegaard, Kerstin Skovgaard, Shila Mortensen,et al. Molecular characterisation of the early response in pigs to experimental infection with Actinobacillus pleuropneumoniae using cDNA microarrays. BMC Genomics,2007,49:11.
[153]Cantrell DA, Smith KA. The interleukin-2 T-cell system:a new cell growth model[J]. Science, 1984,224(4655):1312-1316.
[154]Smith KA. Interleukin-2:inception, impact, and implications[J]. Science,1988,240, (4856): 1169-1176.
[155]金伯泉.医学免疫学[M].北京：人民卫生出版社,第5版,2008：60-65,105-107.
[156]刘艳芳,汤建民.白细胞介素2局部治疗恶性胸腔积液的临床观察[J].中国医药指南,2011,9(9)：54-55.
[157]李丽,张淑霞,韩文宁,等.白细胞介素2、8检测在婴幼儿重症支原体肺炎中的临床价值[J].中国生育健康杂志,2010,21(2)：101-103.
[158]Dooms H, Kahn E, Knoechel B, et al. IL-2 induces a competitive survival advantage in T lymphocytes[J]. J Immunol,2004,172(10):5973-5979.
[159]杜德伟,白宪光,冯志华,等.白细胞介素2提高HBV基因疫苗诱导的体液免疫应答[J].实用肝脏病杂志,2003(2)：78-79.
[160]Waldmann TA. The biology of interleukin-2 and interleukin-15:implications for cancer therapy and vaccine design[J]. Nature Rev Immun,2006,6 (8):595-601.
[161]Waldmann TA, Tagaya Y. The multifaceted regulation of interleukin-15 expression and the role of this cytokine in NK cell differentiation and host response to intracellular pathogens[J]. Annu Rev Immunol,1999,17:19-49.
[162]Wolf SF, Temple PA, Kobayashi M, et al. Clong of cDNA for natural killer cell stimulatory factor, a heterodimeric cytokine with multiple biologic effect of T and natural killer cells[J]. J Immunol,1991,146(9):3074-3081.
[163]Sten As, Podlaski FJ, Hulmes JD, et al. Purincation to homogeneity and partial characterization of cytotoxic lymphocyte maturalion factor from human B-lymphoblastoid cells[J]. Proc Natl Acad sci USA,1990,87(17):6808-6812.
[164]Podlaski FJ, Nanduri VB, Hulmes JD, et al. Molecular characterization of interleukin 12[J]. Arch Biochem Biophys,1992,294(1):230-237.
[165]叶松道,郑晓群,杨锦红,等.巨细胞病毒肝炎患儿IL-12 p403’非翻译区基因多态性研究[J].医学研究杂志,2010,39(12)：92-96.
[166]Oppmann B, Lesley R, Blom B,et al. Novel p19 protein engages IL-12 p40 to form a cytokine, IL-23, with biological activities similar as well as distinct from IL-12[J]. Immunity,13(5):715-25.
[167]袁远英,方强,孙新,等.猪白细胞介素12 p35、p40亚基基因的克隆和序列分析[J].蚌埠医学院学报,2008,33(6)：644-647.
[168]杨瑛,汤绍涛,曹国庆,等.L一12 P35,P40和IFN-γ在胆道闭锁小鼠模型肝脏组织中的表达[J].临床小儿外科杂志,2008,7(6)：26-29.
[169]黄建安,顾冬梅,庄羽美,等.CD40分子在肺癌中的表达及其临床意义[J].江苏医药,2003,29(9)：645-647.
[170]Van Kooten C, Bancherean J. Functions of CD40 on B cells, dendritic cells and other cells[J]. Curropin Immunol.1997,9:330-341.
[171]王淑娟,陈明,李鸿佳,等.脂多糖诱导小鼠肺损伤模型巨噬细胞活化及共刺激分子CD40上调研究[J].中华微生物学和免疫学杂志,2007,27(12)：1076-1080.
[172]Sime PJ, O'Reilly KM. Fibro sis of the lung and other tissues:new concepts in pathogenesis and treatment[J]. Clin Immunol,2001,99(3):308-319.
[173]吴彬,但伶.CD40-CD40L与急性肺损伤[J].重庆医科大学学报,2007：114-115,118.
[174]Suttles J, Evans M, Miller RW, et al. T cell rescue of monocytes from apoptosis:ro le of the CD40-CD40L interaction and requirement for CD40-mediated induction of protein tyrosine kinase activity [J]. J Leukoc Biol,1996,60(5):651-657.
[175]Phipps RP, Stein SH, Roper RL. A new view of pro staglandin E regulation of the immune response[J]. Immunol Today,1991,12(10):349-352.
[176]Zhang-Hoover J, Sutton A, Stein-Streilein J. CD40/CD40 ligand interactions are critical for elicitation of autoimmune-mediated fibrosis in the lung[J]. J Immunol,2001,166(5):3556-3563.
[177]Ishida TK, Tojo T, Aoki T, et al. TRAF5, a novel tumor necrosis factor receptor-associated factor family protein, mediates CD40 signaling[J]. Proc Natl Acad Sci USA,1996,93(18):9437-42.
[178]Clark EA. CD40:a cytokine receptor in search of a ligand[J]. Tissue Antigens,1991,36(1):33-6.
[179]Banchereau J, Bazan F, Blanchard D, et al. The CD40 antigen and its ligand[J]. Annu Rev Immunol,1994,12:881-922.
[180]Cheng G, Schoenberger SP. CD40 signaling and autoimmunity[J]. Curr Dir Autoimmun,2002,5: 51-61.
[181]Dallman C, Johnson PW, Packham G. Differential regulation of cell survival by CD40[J]. Apoptosis,2003,8(1):45-53.
[182]O'Sullivan B, Thomas R. Recent advances on the role of CD40 and dendritic cells in immunity and tolerance[J]. Curr Opin Hematol,2004,10(4):272-8.
[183]Abbas, Lichtman AH. Ch3 Antigen capture and presentation to lymphocytes [J]. Basic Immunology Functions and disorders of the immune system (3rd ed),2009,978(1):4160-4688.
[184]Klein J, Figueroa F. Evolution of the major histocompatibility complex[J]. Crit Rev Immunol, 1986,6(4):295-386.
[185]Kelly PT, Mackinnon RL 2nd, Dietz RV, et al. Postsynaptic IP3 receptor- mediated Ca2+ release modulates synaptic transmission in hippocampal neurons. Brain Res Mol Brain Res,2005, 135:232-48.
[186]Munoz-Chapuli R, Quesada AR, Angel Medina M. Angiogenesis and signal transduction in endothelial cells. Cell Mol Life Sci,2004,61:2224-43.
[187]Zachary I. VEGF signalling: integration and multi-tasking in endothelial cell biology. Biochem Soc Trans,2003,31:1171-7.
[188]Takahashi H, Shibuya M. The vascular endothelial growth factor (VEGF)/VEGF receptor system and its role under physiological and pathological conditions. Clin Sci (Lond),2005,109:227-41.
[189]Hoeben A, Landuyt B, Highley MS, et al. Vascular endothelial growth factor and angiogenesis. Pharmacol Rev,2004,56:549-80.
[190]Matsumoto T, Claesson-Welsh L.VEGF-receptor signal transduction[J]. Trends Biochem Sci, 2003,28:488-94.
[191]Chuaqui RF, Bonner RF, Best CJ, et al. Post-analysis follow-up and validation of microarray experiments[J]. Nat Genet,2002,32:509-534.
[192]Brazma A, Hingamp P, Quackenbush J, et al. Minimum information about a microarray experiment (MIAME)-toward standards for microarray data[J], Nature genetics,2001,29(4): 365-371.
[193]李瑶.基因芯片数据分析与处理[M].北京：化学工业出版社.2006：133-142.
[194]Rockett JC, Hellmann GM. Confirming microarray data--is it really necessary?[J]. Genomics, 2004,83(4):541-549.
[195]Qin LX, Beyer RP, Hudson FN, et al. Evaluation of methods for oligonucleotide array data via quantitative real-time PCR[J]. BMC Bioinformatics,2006,7:23-31.
[196]Etienne W, Meyer MH, Peppers J, et al. Comparison of mRNA gene expression by RT-PCR and DNA microarray [J]. Biotechniques,2004,36(4):618-620,622,624-616.
[197]Dallas PB, Gottardo NG, Firth MJ, et al. Gene expression levels assessed by oligonucleotide microarray analysis and quantitative real-time RT-PCR--how well do they correlate?[J]. BMC Genomics,2005,6(1):59-68.
[198]De Reynies A, Geromin D, Cayuela JM, et al. Comparison of the latest commercial short and long oligonucleotide microarray technologies[Jj]. BMC Genomics,2006,7:51-58.
[199]Morey J S, Ryan J C, Van Dolah F M. Microarray validation:factors influencing correlation between oligonucleotide microarrays and real-time PCR[J], Biological procedures online,2006, 8(1):175-193.
[2]Taylor DJ. Actinobacillus pleuropneumoniae[M]. In:Straw BE, D'Allaire S, Mengeling WL and Taylor DJ, editor. Diseases of swine.8th. Vol.26. Ames Iowa Iowa State University Press,1999. pp.343-354.
[3]Hoflack, G, D Maes, B Mateusen, et al. Efficacy of Tilmicosin Phosphate (Pulmotil Premix) in feed for the treatment of a clinical outbreak of Actinobacillus pleuropneumoniae infection in growing-finishing pigs[J]. J. Vet. Med. B.2001,48:655-664.
[4]Blackall, P.2001. Pleuropneumonia in pigs. Agency for Food and Fibre Sciences. Deartment of Primary Industries, Queensland Government. Available:http://www. dpi. qld. gov. au/pigs/4463. html. Accessed Jan.13,2004.
[5]唐慧稳.猪传染性胸膜肺炎研究现状[J].畜牧兽医杂志,2003,4(22)：19.
[6]陈如明,谢鸣星,李云峰,等.鲁豫地区驻军猪传染性胸膜肺炎血清学调查[J].中国畜禽传染病,1990,(4)：38-40.
[7]汤万倾.我国猪传染性胸膜肺炎的危害及诊断防治研究[J].畜牧兽医科技息,2004(04)：18-19.
[8]Jacques M. Surface polysaccharides and iron-uptake systems of Actinobacillus pleuropneumoniae[J]. Can J Vet Res,2004,68(2):81-85.
[9]NIELSEN R, ANDERSEN L O, PLAMBECK T, et al. Serological characterization of Actinobacillus pleuropneumoniae biotype 2 strains isolated from pigs in two Danish herds [J]. Vet Microbiol,1997,54:35-46.
[10]BLACKALL P J, KLAASEN H L, VAN DEN BOSCH H, et al. Proposal of a new serovar of Actinobacillus pleuropneumoniae:serovar 15[J]. Vet Microbiol,2002,84:47-52.
[11]Chang Y, Yong R, Struck D K. Cloning and characterization of a hamolysin gene from Actinobacillus pleuropneumoniae[J]. DNA,1989, (8):635-647.
[12]Sebunya T N, Saunders J R. Haemophilus pleuropneumoniae infection in swine:a review. J Am Vet Med Assoc,1983,182:1331-1337.
[13]郑芝.某猪场暴发猪接触传染性胸膜肺炎的诊断报告.中国畜禽传染病,1987,4：33-34
[14]高齐瑜,刘尚高,马理武.猪嗜血杆菌胸膜肺炎病实验诊断和病理形态学观察.中国兽医杂志,1988,14：5-7
[15]吕盛喜,贡东栋,马健.猪接触传染性胸膜肺炎的诊断与防治.中国兽医杂志,1992,18：17
[16]杨旭夫,苑士祥,杨留战,等.我国部分地区猪传染性胸膜肺炎病的调查。中国兽医杂志,1993,19：5-6
[17]周以凤,吴硕显.上海市猪胸膜肺炎的血清学调查.中国兽医科技,1993,9：14-15
[18]徐金生,黄宝春,萧军.进口美国种猪首次检出猪嗜血杆菌胸膜肺炎抗体。动物检疫,1988,1：11-13
[19]沈特来,毛少华,俞少麟.从加拿大进口种猪中首次检出猪传染性胸膜肺炎嗜血杆菌病.动物检疫,1989,2：21-23
[20]龚云登,杨长松.鄂西南地区猪传染性胸膜肺炎血清学调查[J].中国兽医杂志,2010,(2)：93-94.
[21]鄢志勇,龙清孟,龚菲,等.种猪场猪传染性胸膜肺炎的血清学监测及防控[J].中国畜牧兽医,2009(12)：135-136.
[22]赖玉兰,田明星,曹三杰,等.猪传染性胸膜肺炎放线杆菌血清5a型ApxⅣA全基因的序列测序及其分子特征[J].四川农业大学学报,2009,27(4)：479-486.
[23]唐文山,李昕.猪传染性胸膜肺炎研究进展[J].动物医学进展,2008,29(2)：98-102.
[24]何启盖,吴斌,吴信明,等.猪接触传染性胸膜肺炎的防制现状.华中农业大学学报,2000,33(增刊)：58-62
[25]陈小玲,杨旭夫,朱士盛.猪传染性胸膜肺炎的流行现状和防制措施.中国兽医杂志,2001,37：33-35
[26]高作信主编.猪病学.北京：中国农业出版社,第三版,2001,178-179
[27]Willson P J, Falk G, Klashinsky S. Detection of Actinobacillus pleuropneumoniae infection in pigs. Can Vet J,1987,28:111-116
[28]Nicolet J. Actinobacillus pleuropneumoniae. In:Leaman A D, Straw B E, Mengeling S, Allaire SD, Taylor D J eds., Diseases of swine. Ames:Iowa State University Press,1992,401-408.
[29]Torremorell M, Pijoan C, Janni IL Walker R, et al. Airborne transmission of Actinobacillus pleuropneumoniae and porcine reproductive and respiratory syndrome virus in nursery pigs. Am J Vet Res,1997,58:828-832.
[30]Vigre H, Angen O, Barfod K La, et al. Transmission of Actinobacillus pleuropneumoniae in pigs under field-like conditions:emphasis on tonsillar colonization and passively acquired colostral antibodies. Vet Microbiol,2002,89:151-159.
[31]尚丽娟.猪传染性胸膜肺炎的防治[J].中国畜牧兽医,2008,35(2)：107-108.
[32]斯特劳[美],阿莱尔[加],蒙加林[美],泰勒[英]主编；赵德明,张中秋,沈建中主译.猪病学(第八版).北京：中国农业大学出版社,2000,357-367
[33]Cruijsen T,Van Leengoed L A, Kamp E M, et al. Suseeptibility to Actinobacillus pleuropneumoniae infection in pigs from all endemically infected herd is related to the presence of toxin-neutralizing antibodies. Vet Microbiol,1995,47:219-228.
[34]Dom P, Haesebrouck F, Kamp E M, et al. NAD-independent Actinobacillus pleuropneumoniae strains:production of RTX toxins and interactions with porcine phagocytes. Vet Microbiol,1994.39:205-218
[35]Chiers K Haesebrouck F, Van Overbeke I, et al. Ducatelle IL Early in vivo interaction of Actinobacillus pleuropneumoniae with tonsils of pigs. Vet Microbiol,1999,68:301-306.
[36]Jacques M, Belanger M, Rov G, et al. Adherence of Actinobacillus pleuropneumoniae to porcine tracheal epithelial cells and frozen lung sections. Vet Microbiol,1991,27:133-143.
[37]Mulks M H, Moxon E R,Bricker J, et al. Examination of Haemophilus pleuropneumoniae for immunoglobulin A protease activity. Infect lmmun,1984,45:276-277
[38]Negrete-Abascal E, Reyes M E, Garcia R M, et al. Flagella and Motility in Actinobacillus pleuropneumoniae. J Bacteriol,2003,185:664-668
[39]Bilinski T. Oxygen toxicity and microbial evolution. Biosystems,1991,24:305-312.
[40]Langford P R, Loynds B M, Kroll J S. Cloning and molecular characterization of Cu,Zn superoxide dismutase from Actinobacillus pleuropneumoniae. Infect lmmun,1996,64:5035-5041.
[41]Bosse J T, Macinnes J I. Urease activity may contribute to the ability of Actinobacillus pleuropneumoniae to establish infection. Can J Vet Res,2000,64:145-150.
[42]毛志亮,王孝荣,李翠苹,等.猪传染性胸膜肺炎的诊断与防治[J].畜牧与兽医,2008,40(3)：112.
[43]逯忠新.猪传染性胸膜肺炎.见：陈焕春主编,规模化猪场疫病监控与净化。北京：中国农业出版社,2000：273-276
[44]Jacobsen M J, Nielsen J P.Development and evaluation of a selective and indicative medium for isolation of Actinobacillus pleuropneumoniae from tonsils. Vet Microbiol,1995,47:191-197.
[45]Sidibe M, Messier S, Lariviere S, et al. Detection of Actinobacillus pleuropneumoniae in the porcine upper respiratory tract as a complement to serological tests. Can J Vet Res,1993,57: 204-208
[46]张蓉蓉,粱旺望,方兵兵,等.免疫磁珠技术分离猪传染性胸膜肺炎放线杆菌.畜牧兽医学报,2008,39：343-348
[47]Fittipaldi N, Broes A, Harel J, et al. Evaluation and field validation of PCR tests for detection of Actinobacillus pleuropneumoniae in subclinically infected pigs. J Clin Microbiol,2003.41: 5085-5093.
[48]Chiers K,Van Overbeke I, Donne E, et al. Detection of Actinobacillus pleuropneumoniae in cultures from nasal and tonsillar swabs of pigs by a PCR assay based on the nucleotide sequence of a dsbE-like gene. Vet Microbiol,2001,83:147-159.
[49]Gram T, Ahrens P, Nielsen J P. Evaluation of a PCR for detection of Actinobacillus pleuropneumoniae in mixed bacterial cultures from tonsils. Vet Microbliol,1996,51:95-104.
[50]Gram T, Ahrens P, Andreasen M, et al. An Actinobacillus pleuropneumoniae PCR typing system based on the apx and omlA genes-evaluation of isolates from lungs and tonsils of pigs. Vet Microbiol,2000,75:43-57.
[51]逯忠新,杨学山,赵卫平,等.猪胸膜肺炎放线杆菌PCR检测方法的建立.中国兽医科技,2004,34：6-8
[52]王牟平,刘思国,王春来,等.猪传染性胸膜肺炎PCR诊断方法的建立.中国预防兽医学报,2003,25： 305-309
[53]刘军发,何启盖,陈焕春,等.用PCR检测猪胸膜肺炎放线杆菌.华中农业大学学报,2001,20：156-158
[54]李鹏,马艳娇,夏伟,等.猪肺炎支原体、多杀性巴氏杆菌和猪胸膜肺炎放线杆菌三重PCR检测方法的建立[J].中国预防兽医学报,2010,(8)：607-610.
[55]徐景峨,蔡一鸣,王璇,等.猪传染性胸膜肺炎放线杆菌PCR检测试剂盒的研制[J].中国畜牧兽医,2010,(4)：209-211.
[56]Cho W S, Chae C. Expression of the apxIV gene in pigs naturally infected with Actinobacillus pleuropneumoniae. J Comp Pathol,2001,125:34-40.
[57]Cho W S , Chae C. PCR detection of Actinobacillus pleuropneumoniae apxIV gene in formalin-fixed paraffin-embedded lung tissues and comparison with in situ hybridization. Lett Appl Microbiol,2003,37:56-60
[58]Rosendal S, Carpenter D S,Mitchell W R, et al. Vaccination against pleuropneumonia of pigs caused by Haemophilus pleuropneumoniae. Can Vet J 1981,22:34-35
[59]王贵平.猪传染性胸膜肺炎免疫荧光和PCR诊断方法研究及apxIVA的克隆与序列分析。[硕士学位论文].武汉：华中农业大学图书馆,2004
[60]Hoffman L. Actinobacillus(Haemophilus) pleuropneumoniae:use of coagglutination and complement fixation to determine the relationship between presence of organism and antibody titer in slaughter house pigs. J Vet Diagn Invest,1989,1:12-15.
[61]Nielsen R. Haemophilus pleuropleumoniae(Actinobacillus pleuropleumoniae)serotype 8,3 and 6 serological response and cross immunity in pigs. Nord vet Med,1985,37:217-227.
[62]Lombin L H, Rosendal S and Mitchell W R. Evalution of the complement fixation test for the diagnosis of pleuropneurnoniae of swine caused by Haemophilus pleuropneumoniae. Can J comp Med,1982,46:109-114.
[63]Mittal K R, Higgins R, Lariviere S. Evaluation of slide agglutination and ring precipitation tests for capsular serotyping of Haemophilus pleuropneumoniae. J Clin Microbiol,1982,15:1019-1023
[64]逯忠新,鲁炳义用间接血凝试验检测猪传染性胸膜肺炎.中国兽医科技,1999,29-25-27
[65]Mittal K R, Higgins R, Lariviere S, et al. A 2-mereaptoethanol tube agglutination test for diagnosis of Haemophilus pleuropneumoniae infection in pigs. Am J Vet Res,1984,45:715-719.
[66]Mittal K R, Higgins R, Lariviere S. An evaluation of agglutination and coagglutination techniques for serotyping of Haemophilus pleuropneumoniae isolates. Am J Vet Res,1987,48:219-226
[67]Rapp V J, Ross R F, Erickson B Z.Serotyping of Haemophilus pleuropneumoniae by rapid slide agglutination and indirect fluorescent antibody tests in swine. Am J Vet Res,1985,46:185-192
[68]Gunnarsson A, Hurvell B, Biberstein E L. Serologic studies of Haemophilus parahaemolyticus (pleuropneumoniae):antigenic specificity and relationship between serotypes. Am J Vet Res, 1978,39:1286-1292
[69]Gunnarsson A. Serological studies on porcine strains of Haemophilus parahaemolyticus (pleuropneumoniae):extraction of type-specific antigens. Am J Vet Res,1979,40:469-472.
[70]Giese S B, Stebaek E, Nielsen R. Identification of Actinobacillus pleuropneumoniae serotype 2 by monoclonal or polyclonal antibodies in latexagglutination tests. Acta Vet Scand,1993,34: 223-225.
[71]Inzana T J. Simplified procedure for preparation of semitized latex particles to detect capsular polysaccharides:application to typing and diagnosis of Actinobacillus pleuropneumoniae. J Clin Microbiol,1995,33:2297-2303.
[72]Hommez J, Devriese L A, Castryck F, et al. Slide precipitation:a simple method to type Actinobacillus(Haemophilus)pleuropneumoniae. Vet Microbiol,1990,24:123-126.
[73]Bosse J L, Johnson RP, Rosendal S. Serodiagnosis of pleuropneumonia using enzymed-linked immunosorbent assay with capsular polysaccharide antigen of Actinobacillus pleuropneumoniae serotype 1,2,5 and 7. Can J Vet Res,1990,54:427-431.
[74]Radacovici S,Lallier R, Lariviere S, et al.Biochemical characterization of an antigenic saline extract of Actinobacillus pleuropneumoniae serotype 5 and identification of a serotype-specific antigen for ELISA serodiagnosis. Vet Microbiol,1992,30:369-385
[75]Radacovici S,Gottschalk M,Dubreuil J D.Recovery of long-chain lipopolysaccharides from liquid culture of Actinobacillus pleuropneumoniae(serotype 5)for ELIS A serodiagnosis. Vet Res,1995, 26:63-67
[76]Gottschalk M, De LaSalle F, Radacovici S, et al. Evaluation of long chain lipopolysaccharides(LC-LPS) of Actinobacillus pleuropneumoniae serotype 5 for the serodiagnosis of swine pleuropneumonia. Vet Microbiol,1994,38:315-327
[77]Nielsen N, Plambeck T, Foged N T. Blocking enzyme—linked immunosorbent assay for detection of antibodies to Actinobacillus pleuropneumoniae serotype 2. J Clin Microbiol,1991,29: 794-797
[78]Nielsen R, Plambeck T, Foged N T. Blocking enzyme-linked immunosorbent assay for detection of antibodies against Actinobacillus pleuropneumoniae serotype 8. Vet Microbiol,1993,34:131-138
[79]Andresen L O, Klausen J, Barfod K Sorensen V. Detection of antibodies to Actinobacillus pleuropneumoniae serotype 12 in pig serum using a blocking enzyme-linked immunosorbent assay. Vet Microbiol,2002,89:61-67
[80]Leiner G, Franz B, Strutzberg K, et al.A novel enzyme-linked immunosorbent assay using the recombinant Actinobacillus pleuropneumoniae ApxII antigen for diagnosis of pleuropneumonia in pig herds. Clin Diagn Lab Immunol,1999,6:630-632.
[81]Nielsen R,van den Bosch J F, Plambeck T, et al. Evaluation of an indirect enzyme-linked immunosorbent assay(ELISA)for detection of antibodies to the Apx toxins of Actinobacillus pleuropneumoniae. Vet Microbiol,2000, 71:81-87
[82]Dreyfus A, Schaller A, Nivollet S, et al. Use of recombinant ApxIV in serodiagnosis of Actinobacillus pleuropneumoniae infections,development and prevalidation of the ApxIV ELISA. Vet Microbiol,2004,99:227-238.
[83]黄红亮,周锐,陈美玲,等.胸膜肺炎放线杆菌apxIVA基因的克隆与表达及间接ELISA方法的建立.生物工程学报,2005,21：294-299
[84]Hu D H, Hart H Y, Zhou R, et al. Gold(III) enhanced chemiluminescence immunoassay for detection of antibody against ApxIV of Actinobacillus pleuropneumoniae. Analyst,2OO8,133: 768-773.
[85]Sheng Z H,Han H Y,Hu D H, et al. Quantum dots-gold(Ⅲ)-based indirect fluorescence high-throughput screening of APP. Chem Comm,2009, DOI:10.1039/b822921j
[86]Pol J M, Van Leengoed L A, Stockhofe N, et al. Dual infections of PRRSV/influenza or PRRSV/Actinobacillus pleuropneumoniae in the respiratory tract. Vet Microbiol,1997,55: 259-264
[87]张彦明,张耀相,刘春花,等.胸膜肺炎放线杆菌的分离鉴定及免疫预防.中国兽医科技,2002,32：21-22
[88]李清艳,翟向和,钟秀会,等.附红细胞体自然感染病猪血浆NO、SOD和MDA的变化[J].动物医学进展,2005,26(2)：92-94.
[89]孙存普,张建中,段绍瑾.自由基生物学导论[M].安徽合肥,中国科学技术出版社,1999.
[90]毕晋明,郑姗姗,张敏红,等.日粮中添加不同浓度吡啶甲酸铬对生长肥育猪氧化作用的影响[J].动物营养学报,2008,20(5)：561-566.
[91]龙建纲,王学敏,高宏翔,等.丙二醛对大鼠肝线粒体呼吸功能及相关脱氢酶活性影响[J].第二军医大学学报,2005,26(10)：1131-1135.
[92]莫简.自由基在生物体系中的作用：普通中间介质或非特异性调节剂[J].自由基生命科学进展,1999(7)：7-10.
[93]Vander Vliet A, Cross CE. Oxidants, nitrosants, and the lung[J]. Am J Med,2000,109(5): 398-421.
[94]李华强,史源,潘捷,等.内源性一氧化氮在感染性肺炎病理过程中的作用[J].第三军医大学学报,1999,21(3)：206-208.
[95]Yu J C, Jiang Z M, Li D M. Glutamine:a precusor of glutathione and its efect on liver[J]. World J Gastroentero,1999,5(2):143-146.
[96]Alverdy AM. Effects of glutamine-supplemented diets on immunology of the gut[J]. Parenter Enteral Nutr,1990,(14):109-113.
[97]赵晶,康世良.健康仔猪口服亚硒酸钠后血液硒浓度与抗氧化系统动态变化规律的研究[J].畜牧兽医学报,2003,34(6)：554-557.
[98]Abu-khaderAA, BihoYY. Exposure of human meutrophils to oxygen redicals causes loss of deformability. lipid peroxidation, protein degradation, respiratory burst activation and loss of migration[J]. Clin Hemorheol Microcircula,2002,27(1):57-66.
[99]李敏,崔伟,彭西,等.高铜对雏鸡脑组织抗氧化酶活性的影响[J].畜牧兽医学报,2010,41(2)：220-22
[100]Vukic Z. Gluteal gunshot wounds [J]. Mil Med,2000,165(3):237-239.
[101]llie N, Petricevic A, Tanfara S, et al. War-mjuriestothe chest[J]. Acta Chir Hung,1999, 38(1):43-47.
[102]Desjardins F, Balligand JL. Nitric oxide-dependent endothelial function and cardiovascular disease[J]. Acta Clin Belg,2006,61(6):326-334.
[103]Yetik-Anacak G, Catravas JD. Nitric oxide and the endothelium:history and impact on cardiovascular disease[J]. Vaseul Pharmacol 2006,45(5):268-276.
[104]Pheng LH, Francoeur C, Denis M. The involvement of nitric oxide in a mouse model of adult respiratory distress syndrome [J]. Inflammation,1995,19:599-610.
[105]Bloomfield GL, Holloway S, Ridings PC, et al. Pretreatment with inhaled nitric oxide inhibits neutrophil migration and oxidative activity resulting in attenuated sepsis-induced acute lung jnjury[J]. Crit Care med,1997,25:584-593.
[106]Gaston B, Drazen JM, Loscalzo J, et al. The biology of nitrogen oxides in the airways[J]. Am J Respir Crit Care Med,1994,149:538-51.
[107]Btmser, Minlseh A, Fleming F, et al. Mechanism of nitric oxidere lease from the vascular endothelium. Circulation.1993,97(supp1):18.
[108]Bingisser R M. Hoh P G. Immunomod ulating mechanisms in the lower respiratory tract:nitric oxide mediated interactions between alveolar macmphages, epithelial cells and T-cells[J]. Swiss Med Wkly,2001; 131(13-14):171-179.
[109]刘晓宇,高和.一氧化氮对呼吸系统炎症与病原微生物影响研究进展[J].国际呼吸杂志,2007,27(5)：338-341.
[110]王宁飞,蔡富娟,刘帅,等.过表达Rab7基因对LPS和PolyI:C活化的巨噬细胞RAW264.7中iNOS/NO的影响[J].中国免疫学杂志,2011,27(6)：497-501.
[111]赵丽,杨帆,彭西,等.高铜对雏鸭肝羟自由基和一氧化氮产生的影响.中国兽医科学2008.38(09)：787-790.
[112]Bian K, Harari Y, Zhong M. Down-regulation of inducible nitric-oxide synthase (NOS-2) during Parasite-induced gut Inflammation:a Path to identify a selective NOS-2 inhibitor. Mol Pharmacol, 2001,59:939-947.
[113]程安玮,金征宇,万发春.甘草多糖xCd,鼠腹腔巨噬细胞NO、iNOS及iNOS mlLNA表达的影响[J].食品科学,2009,30(21)：351-352.
[114]蒋波,楚正绪.一氧化氯与炎症[J].国外医学一生理病理科学与临床分册,1998,18(1)：44-47.
[115]姜勇.内毒素激活内皮细胞的信号机制的研究进展[J].中华医学杂志,1999,79：76-79.
[116]Selye. H A. Syndrome produced by diverse nocuous agents [J]. Nature,1936:32.
[117]Mader T L. Environm ental stress in confined beef cattle [J]. Anim Sci,2003,81:110-119.
[118]Raymond C R, Wilkie B N. Th-1/Th-2 type cytokine profiles of pig T-cells cultured with antigen-treated monocyte-derived dendritic cells[J]. Vaccine,2004,22:1016-1023.
[119]G. B. Toews, Cytokines and the lung, Eur. Respir. J. Suppl.34 (2001), pp.3s-17s.
[120]Duh EJ, WJ Maury, TM Folks, et al. Tumor necrosis factor alpha activates human immunodeficiency virus type 1 through induction of nuclear factor binding to the NF-kappa B sites in the long terminal repeat. Proc Natl. Acad Sci USA,1989,86:5974-5978.
[121]Kronke, M., S. Schutze, P.Scheurich, et al. TNF signal transduction and TNF-responsive genes[J]. Immunol. Ser,1992,56:189-216.
[122]Lowenthal, J. W., D. W. Ballard, et al. Tumor necrosis factor alpha induces proteins that bind specifically to kappa B-like enhancer elements and regulate interleukin 2 receptor alpha-chain gene expression in primary human Tlymphocytes. Proc. Natl. Acad. Sci. USA,1989,86:2331-2335.
[123]Osborn, L, S. Kunkel, G. J. Nabel. Tumor necrosis factor alpha and interleukin 1 stimulate the human immunodeficiency virus enhancer by activation of the nuclear factor kappa B. Proc. Natl. Acad. Sci. USA,1989,86:2336-2340.
[124]Schutze S, K.Potthoff, T. Machleidt, et al. TNF activates NF-kappa B by phosphatidylcholine-specific phospholipase C-induced "acidic" sphingomyelin breakdown[J]. Cell,1992,71:765-776.
[125]Bussolino.F, G. Camussi, C. Baglioni. Synthesis and release of platelet-activating factor by human vascular endothelial cells treated with tumor necrosis factor or interleukin 1 alpha[J]. J. Biol. Chem,1988,263:11856-11861.
[126]Libby.P, J.M Ordovas, K.R. Auger, et al. Endotoxin and tumor necrosis factor induce interleukin-1 gene expression in adult human vascular endothelial cells[J]. Am. J. Pathol,1986, 124:179-185.
[127]PP Nawroth, I Bank, D Handley, et al. Tumor necrosis factor/cachectin interacts with endothelial cell receptors to induce release of interleukin 1[J]. J. Exp. Med,1986,163:1363-1375.
[128]Pober J.S, M.Gimbrone, LA Lapierre, et al. Overlapping patterns of activation of human endothelial cells by interleukin 1, tumor necrosis factor, and immuneinterferon[J]. J. Immunol, 1986,137:1893-1896.
[129]Molnar L, Berki T, Hussain A, et al. Detection of TNFalpha expression in the bone marrow and determination of TNFalpha production of peripheral blood mononuclear cells in myelodysplastic syndrome[J]. Pathol Oncol Res.2000;6:18-23.
[130]Chrysant SG. The path physiologic role of the brain Renin-Angiotensin system in stroke protection:clinical implications [J]. J Clin Hypertens(Greenwich),2007,9(6):454-459.
[131]Di Giovine F S, Nuki GL. Tumor nerosis factor in synovial exudates. An Rheum Dis,1988,47: 768-772.
[132]Maier SF, Watkins LR. Cytokines for psychologists:implications of bidirectional immune-to-brain communication for understanding behavior, mood, and cognition[J]. Psychol Rev 1998,105:83-107.
[133]Dinarello CA. Biologic basis for interleukin-1 in disease[J]. Blood,1996,87:2095-2147.
[134]李戎,闰智勇,李文军,等.肺纤维化发生与防治机制的重要因素-细胞因子作用机制研究[J].中国中医药,2004,6：23.
[135]Marz W, Wieland H. HMG-CoA reeducates inhibition:antiinflammatory effects beyond lipid lowering[J]. Herz,2000,25(2):117-125
[136]王光辉,陈传兵,王献武.支气管肺炎患儿治疗前后血清IGF.IL-I、IL-6、1L-8、和hs-CRP检测的临床意义[J].放射免疫学杂志,2009,22(4)：337-338.
[137]陈侃侃.肺炎支原体肺炎患儿血清hs-CRP、IL-6和IL-8检测的临床意义[J].放射免疫学杂志,2011,24(3)：266-267.
[138]Nagahori T, Uohi M, Matsumoto K, et al. Interferon-gitnma upregulates the C-Met/hepatocyte growth factor receptor expression in alveolar epithelial cells[J]. Am J Respir Cell Mol Biol,1999, 21:490-497.
[139]Bajwa EK, Ayas NT, Schuher M, et al. Interferon-lb therapy in idiopathic pulmonary fibrosis:a metaanalysis[J]. Chest,2005,128:203-206.
[140]Cantrell DA, Smith KA. The interleukin-2 T-cell system:a new cell growth model[J]. Science,1984,224 (4655):1312-1316.
[141]Smith KA. Interleukin-2:inception, impact, and implications[J]. Science,1988,240(4856): 1169-1176.
[142]金伯泉.医学免疫学[M].第5版.北京：人民卫生出版社,2008：60-65,105-107.
[143]DOOMS H, KAHN E, KNOECHEL B, et al. IL-2 induces a competitive survival advantage in T lymphocytes[J]. J Immunol,2004,172(10):5973-5979.
[144]李爱华,曹祥山.IL-2与CD4+CD5+调节性T细胞的相关研究进展[J].医学综述,2007,13(9)：652-653.
[145]Trojanowska M. Role of PDGF in fibrotic diseases and systemic sclerosis[J]. Rheumatology, 2008,47(5):2-4.
[146]卫张蕊,周国锋.炎症细胞和细胞因子在肺纤维化中作用的研究进展[J].细胞与分子免疫学杂志,2005,21(Supp1)：85-87.
[147]Li X, Hu Y, Jin Z, et al. Silica-induced TNF-alpha and TGF-betal expression in RAW 264.7 cells are dependent on Src-ERK/AP-1 pathways[J]. Toxicol Mech Methods,2009,19(1):51-58.
[148]姚武,王治明,王绵珍,等.煤工尘肺患者血清细胞因子及氧化损伤水平研究[J].四川大学学报(医学版),2005；36(4)：510-512.
[149]Santana A, Saxena B, Nancy A, et al. Increased expression of TGF-B1 in bleomycin-induced pulmonary fibrosis[J]. Am J Respir Cell Mol Biol,1995,13(1):34-44.
[150]Gulumian M, Borm PJ, Vallyathan V, et al. Mechanistically identified suitable biomarkers of exposure, effect, and susceptibility for silicosis and coal-worker's pneumoconiosis:a comprehensive review[J]. J Toxicol Environ Health B Crit Rev,2006,9(5):357-395.
[151]Petersen H H, Nielsen J P, Heegaard P M H. Application of acute phase protein measurements in veterinary clinical chemistry [J]. Veterinary Research,2004,35:163-187.
[152]Malle E, De Beer F C. Human serum amyloid A (SAA) protein:a prominent acute-phase reactant for clinical practice. Eur J Clin In-vest,1996,26(6):427-435.
[153]Yip T, Chan J, Cho W, et al. Protein chip array profiling analysis in patients with severe acute respirator syndrome identified serum amyloid A protein as a biomarker potentially useful in monitoring the extent of pneumonia[J]. Clin Chem,2005,51:47-55.
[154]Kokubun M, Imafuku Y, Okada M, et al. Serum amyloid A (SAA) concentration varies among rheumatoid arthritis patients estimated by SAA/CRP ratio. Clin Chimica Acta,2005,360:97-102.
[151]Bing Z, Reddy SA, Ben Y, et al. Purification and eharaeterization of the serum amyloid A3 enhancer factor [J]. J Biol Chem,1999,274(35):24649.
[152]Boylan MT, Crockard AD, Duddy ME. Interferon-betala administration results in a transient increase of 8enlm amyloid A protein and C-reactive protein:comparison with other markers of inflammation[J]. Immunol Lett,2001,75(31):191.
[153]Volanakis JE. Human C-reactive protein:expression, structure, and function. Mol Immunol,2001, 38:189-197.
[154]叶应妩,王毓三,申子瑜.全国临床检验操作规程[M].南京：东南大学出版社,第3版,2006：590.
[155]陈侃侃.肺炎支原体肺炎患儿血清hs-CRP、IL-6和IL-8检测的临床意义[J].放射免疫学杂志,2011,24(3)：266-267.
[156]曹嫚,何少为.肺炎支原体患儿血清中TNF-α和Hs-CRP水平联合测定的临床意义[J].中外医学研究,2011,9(18)：24-24.
[157]Eckersall PD, Saini PK, McComb C. The acute phase of acid soluble glycoprotein-lacid glycoprotein, ceruplasmin, haptoglobin ang C-reactive protein in the pigin a transient increase of[J]. Vet Immunol Immunopathol,1996,51:377-385.
[158]Heegaard PMH, Klausen J, Nielsen JP, et al. The porcine acute phase response to infection witn Actinobacillus pleuropneumoniae. Haptoglobin, C-reactive portein, major acute phase portien and serum amyloid A protein are sensitive indicators of infection. Comp Biochem Physiol[J],1998, 119B:365-373.
[159]Lauritzen B, Lykkesfeldt J, Skaanild MT, et al. Putative biomarkers for evaluating antibiotic treatment:an experimental model of porcine Actinobacillus pleuropneumoniae infection[J]. Res Vet Sci,2003,74:261-270.
[160]Lauritzen B, Lykkesfeldt J, Skaanild MT et al. Putative biomarkers for evaluating antibiotic treatment:an experimental model of porcine Actinobacillus pleuropneumoniae infection[J]. Res Vet Sci,2003,74:261-270.
[161]Hulten C, Johansson E, Fossum C, et al. Interleukin-6, serum amyloid A and haptoglobin as marker of treaqtment efficacy in pigs experimentally infected with Actinobacillus pleuropneumoniae[J]. Vet J,2003,159:194-200.
[162]BRANDTZAEG P. Molecular and cellular aspects of the secretory immunoglobulin system [J]. A pmis,1995,103(1):1-19.
[163]MACPHERSON AJ, McCOYKD, JOHANSENFE, et al. The immune geography of IgA induction and function [J]. Mucosal Immunology,2008,1:11-22.
[164]WOOF J M, KERR M A. The function of immunoglobulin A in immunity [J]. J Pathol,206, 208(2):270-282.
[165]赵武述.免疫平衡研究及其临床意义[M].北京：科学出版社,2005：90.
[166]龚非力.医学免疫学[M].北京：科学出版社,2000：39.
[167]CRAWLEY A, WILKIE B N. Porcine Ig isotypes:function and molecular characteristics [J]. Vaccine,2003,21 (21-22):2911-2922.
[168]周武,汪德清.红细胞功能检测方法的研究进展[J].中国输血杂志,2010,23(8)：648-650.
[169]Nelson R A. The immune phenomenon: An immunologically specific reaction between microorganisms and erythrocyte leading to enhanced phagocytosis [J]. Science,1953,118:773.
[170]SIEGEL I, TIAN Liu-liu, NORBERT G. The red-cell immune system [J]. Lancet,1981,318(9): 556-559.
[171]Siegel I, Gleicher N. Red cell immune adherence (RCIA):its application in cancer and autoimmune disease. Immunol Commun.1981;10(4-5):433-449.
[172]郭峰,虞紫茜,赵中丰,等.红细胞免疫功能的初步研究[J].中华医学杂志,1982,62(12)：715-716.
[173]张德成,陈思义,李进昌,等.动物红细胞免疫功能的研究[J].畜牧兽医学报,1992,23(3)：285-288.
[174]王旭东,饶光荣,李正甫,等.11类哺乳类和鸟类动物红细胞免疫功能的对比研究[J].畜牧兽医学报.1995.26(6)：524-529.
[175]张德成,陈思义,吴润培,等.动物红细胞C3b受体测定研究[A].南京：南京大学出版社.1993.123-125.
[176]王旭东,饶家荣,王生奎.九种红细胞免疫功能研究分析和意义[A].南京：南京大学出版社.1993.125-127
[177]胡金川.红细胞免疫及其临床应用研究进展[J].国际检验医学杂志,2008,29(7)：621-622.
[178]彭益,陈作友.尿毒症患者血液透析对红细胞免疫功能与脂质过氧化关系的研究[J].中国健康月刊：学术版,2010,29(7)：30-32.
[179]夏礼斌,赵咏莉,华强,等.2型糖尿病肾病患者红细胞免疫功能研究[J].中国医药指南,2010,8(22)：7-8.
[180]赵红霞.手足口病患儿红细胞免疫功能与脂质过氧化关系的探讨[J].放射免疫学杂志,2010,23(3)：343-344.
[181]章惠刚,付美丽,赵沛光,等.病毒性心肌炎患者外周血红细胞免疫功能的变化和血清cTnl水平的相关性分析[J].放射免疫学杂志,2010(2)：203-205.
[182]侯海锋,包永占,李茜,等.当归对鸡红细胞免疫功能的影响[J].中国兽医杂志,2009(4)：54-56.
[183]李晓卉,殷中琼.青刺果黄酮对鸡红细胞免疫及外周血淋巴细胞免疫功能的影响[J].中国兽医杂志,2009(3)：43-44.
[184]伊鹏霏,付本懂,张森,等.蓝黄青口服液对鸡传染性喉气管炎疫苗免疫的影响[J].中国家禽,2009(5)：16-19.
[185]彭西,柏才敏,李敏,等.高硒对雏鸡红细胞免疫功能红细胞总数及血红蛋白含量的影响[J].中国兽医科学,2008,38(6)：535-539.
[186]刘福堂,李艳飞,顾庆云.铝中毒对蛋鸡白细胞和红细胞免疫功能的影响[J].中国畜牧兽医,2007,34(10)：70-72.
[187]方静,崔恒敏,彭西.锌中毒对天府肉鸭红细胞免疫功能的影响[J].中国兽医科技,2004,34(9)：34-36.
[188]崔恒敏,彭西,黎德兵,等.高锌对雏鹅红细胞免疫功能的影响[J].中国兽医学报,2004,24(6)：576-578.
[189]陈龙,殷定忠,庞训胜,等.雏鸡在感染IBDV后红细胞免疫功能变化的研究[J].畜牧兽医学报,1998,29(3)：280-284.
[190]李清艳,翟向和,吕建存,等.附红细胞体感染对猪红细胞免疫功能的影响[J].中国兽医科技,2004,34(4)：46-48.
[191]富艳玲,王德志,王晓旭,等.中药乳膏剂对乳房炎奶牛非特异性免疫和红细胞免疫功能的影响[J].中国兽医杂志,2008,44(7)：51-53.
[192]李睿文,宋春丽,李铁拴,等.康贝对隐性乳房炎奶牛红细胞免疫的影响[J].中国兽医杂志,2005,41(6)：23-25.
[193]王丽,剡根强,王静梅.绵羊自然感染附红细胞体对绵羊红细胞的影响[J].石河子大学学报：自然科学版,2006,24(1)：108-111.
[194]温立斌,何孔旺,杨汉春,等.类圆环病毒因子P1感染对猪外周血T淋巴细胞含量和增殖活性的影响[J].华北农学报,2009,24(B08)：1-4.
[195]屈雪琪,赵建增,梁智选,等.流式细胞术检测不同试验条件对猪T淋巴细胞亚群的影响[J].中国畜牧兽医,2011,38(4)：101-104.
[196]徐鹏,杨桂连,刘孝刚,等.仔猪人工感染猪囊尾蚴后外周血中淋巴细胞亚群的动态变化研究[J].吉林农业大学学报,2007,29(4)：440-442.
[197]ZUCJERMANN F A, GASKINS H R. Distribution of CD4/CD8 double-positive T lymphocytes in mucosa-associated lymphoid tissues[J]. Immunology,1996,87:493-499.
[198]SUMMERFIELD A, RZIHA H J, SAALMULLER A. Functional characterization of porcine CD4+, CD8+ extrathymic T lymphocytes [J]. Cellular Immunology,1996,168:291-296.
[199]YANG H, OURA CA L, KIRKHANM, et al. preparation of monoclonal anti-porcine crh antibodies and preliminary characterization of porcine T lymphccytes[J]. Immunology,1996,88: 577-585.
[200]胡永明,赵明秋,陈立军,等.猪瘟病毒感染对猪外周血T淋巴细胞亚群及TNF-α和IFN-γ的影响[J].中国预防兽医学报,2011,33(2)：126-129.
[201]沈艳,华修国,崔立,等.猪链球菌2型两代表性分离株感染小型猪后对部分免疫指标的影响[J].畜牧兽医学报,2008,39(12)：1721-1730.
[202]张振斌,蒋宗勇,等.超早期断奶应激对仔猪T淋巴细胞亚群的影响[J].中国畜牧杂志,1999,35(3)：16-18.
[203]王珊,廖杰,于立方,等.流式细胞术快速植测T细胞亚群和NKT细胞亚群的免疫荧光变化[J].现代科学仪器,2010(3)：95-97.
[204]孙金福,史子学,郭焕成,等.猪瘟病毒感染猪外周血白细胞凋亡及CD4+和CD8+T淋巴细胞亚群的变化[J].中国兽医科学,2008,38(4)：342-345.
[205]李华,杨汉春,等.猪繁殖与呼吸综合征病毒感染仔猪淋巴细胞亚群的动态[J].中国免疫学杂志,2001,17(4)：208-211.
[206]顾有方.猪生殖-呼吸道综合征病毒感染猪淋巴细胞亚群的变化[J].国外兽医学：畜禽传染病,1998,18(3)：47-49.
[207]Williams NH. StahlyTS, Zimmerman DR. Effect of chronic immune system activation on the rate, efficiency and composition of growth and lysine needs of pigs fed from 6 to 27 kg[J]. J Anita Sci, 1997a,75:2463-2471.
[208]W illiams N H. Stahly T S. Zimmerman D R. Effect of chronic imm une system activation on body nitrogen retention, partial efficiency of lysine utilization and lysine needs of pigs[J]. J Anim Sci,1997b,75:2472-2480.
[209]Kim H M, Han S B, Oh G T, Kim Y H. Stimulation of humoral and cell mediated immunity by polysaccharide from mushroom Phellinus linteus. International Journal of Immunopharmacology, 1996,18:295-303.
[210]季敬璋,彭颖,吕建新.牛膝多糖体外诱导人T细胞表达IFN-γ和IL-4蛋白的机制探讨.中国免疫学杂志,2003,19(9)：611-613.
[211]彭西,崔赘,杨帆,等.日粮高硒对雏鸡外周血T淋巴细胞增殖功能和亚群的影响.畜牧兽医学报,2009,40(2)：256-26.
[212]张佩君.猪外周血单个核细胞和植物血凝素对猪淋转试验的影响[J].陕西教育学院学报,2007,23(1)：88-90,111.
[213]李瑶.基因芯片与功能基因组[M].化学工业出版社,2004.
[214]李瑶,陈菊祥.基因芯片的制备研究[J].第二军医大学学报.2000,21(009)：812-814.
[215]Auer H, Newsom DL, Kornacker K. Expression Profiling Using Affymetrix GeneChip Microarrays[J]. Methods Mol Biol.2009,509:35-46.
[216]Katagiri F, Glazebrook J. Overview of mRNA expression profiling using DNA microarrays[J]. Curr Protoc Mol Biol.2009, Chapter 22:Unit 22-24.
[217]Schena M, Shalon D, Heller R, et al. Parallel human genome analysis:microarray-based expression monitoring of 1000 genes[J]. Proc Natl Acad Sci USA,1996,93(20):10614-10619.
[218]Chitko-McKown CG, Fox JM, Miller LC, et al. Gene expression profiling of bovine macrophages in response to Escherichia coli 0157:H7 lipopolysaccharide[J]. Dev Comp Immunol,2004,28(6): 635-645.
[219]Dvorak CM, Hyland KA, Machado JG, et al. Gene discovery and expression profiling in porcine Peyer's patch [J]. Vet Immunol lmmunopathol,2005,105(3-4):301-315.
[210]Gerhold D, Lu M, Xu J, et al. Monitoring expression of genes involved in drug metabolism and toxicology using DNA microarrays[J]. Physiol Genomics.2001,5(4):161-170.
[211]Behr MA, Wilson MA, Gill WP, et al. Comparative genomics of BCG vaccines by whole-genome DNA microarray[J]. Science,1999,284(5419):1520-1523.
[212]Manger ID, Relman DA. How the host 'sees' pathogens:global gene expression responses to infection[J]. Curr Opin Immunol.2000,12(2):215-218.
[213]Klein RJ, Zeiss C, Chew EY, et al. Complement factor H polymorphism in age-related macular degeneration[J]. Science.2005,308(5720):385-389.
[214]Morimoto M, Zarlenga D, Beard H, et al. Ascaris suμm:cDNA microarray analysis of 4th stage larvae (L4) during self-cure from the intestine [J]. Exp Parasitol.2003,104(3-4):113-121.
[1]Taylor DJ. Actinobacillus pleuropneumoniae. In:Straw BE, D'Allaire S, Mengeling WL and Taylor DJ, editor. Diseases of swine.8th. Vol.26. Ames, Iowa, Iowa State University Press; 1999. pp:343-354.
[2]Wallgren P, Segal T, Morner AP, et al. Experimental infections with Actinobacillus pleuropneumoniae in pig-I. Comparison of five different parenteral antibiotic treaments[J]. J Vet Med B,1999,46:249-260.
[3]Fodor L, Reeve-Johnson L, Hodge A, et al. Efficacy evalution of the use of oral rilmicosin in pneumonic calves[J]. Immunol Immunopathol,2000,51:377-385.
[4]Liu JZ, Fung KF, Chen ZL, et al.Pharmacokinetics of Florfenticol in Healthy pigs annd pigs Experimentally Infected with Actinobacillus pleuropneumoniae[J]. Antimicrob Agents Ch,2003,47:820-823.
[5]杨旭夫,彭发泉.我国猪胸膜肺炎嗜血杆菌感染症的确定和诊断[J].畜牧兽医学报,1990,(03)：243-245.
[6]Hoflack G, D. Maes, B. Mateusen, et al. De Kruif. Efficacy of Tilmicosin Phosphate (Pulmotil Premix) in feed for the treatment of a clinical outbreak of Actinobacillus pleuropneumoniae infection in growing-finishing pigs[J]. J. Vet. Med. B,2001,48: 655-664.
[7]Blackall, P.2001. Pleuropneumonia in pigs. Agency for Food and Fibre Sciences. Deartment of Primary Industries, Queensland Government. Available: http://www.dpi.qld.gov.au/pigs/4463. html. Accessed Jan.13,2004.
[8]陈如明,谢鸣星,李云峰,等.鲁豫地区驻军猪传染性胸膜肺炎血清学调查[J].中国畜禽传染病,1990,(4)：38-40.
[9]邬捷,姜永康.四川猪嗜血杆菌胸膜肺炎血清学调查[J].动物检疫,1990(6)：21-22.
[10]汤万倾.我国猪传染性胸膜肺炎的危害及诊断防治研究[J].畜牧兽医科技信息.2004(04)：18-19。
[11]Jacques M. Surface polysaccharides and iron-uptake systems of Actinobacillus pleuropneumoniae [J]. Can J Vet Res,2004,68(2):81-85.
[12]张立昌.猪传染性胸膜肺炎研究进展[J].养猪,2001(1)：40-42。
[13]祝永凯,李文正,王成达,等.传染性胸膜肺炎的诊治[J].2010,31(1)：43.
[1]Baarsch M J, Foss D L, Murtaugh M P. Pathophysiologic correlates of acute porcine pleuropneumonia[J]. American Journal of Veterinary Research,2000,61(6):684-690.
[2]Taylor D J. Actinobacillus pleuropneumoniae. Diseases of swine[M]. Ames, Iowa: Iowa State University Press,1999:343-354.
[3]杨旭夫,彭发泉.我国猪胸膜肺炎嗜血杆菌感染症的确定和诊断[J].畜牧兽医学报,1990,21(3)：243-245.
[4]唐慧稳.猪传染性胸膜肺炎研究现状[J].畜牧兽医杂志,2003,22(4)：19-21.
[5]陈如明,谢鸣星,李云峰,等.鲁、豫地区驻军猪传染性胸膜肺炎血清学调查[J].中国畜禽传染病,1990(4)：38-40.
[6]邬捷,姜永康,曹国文,等.四川猪嗜血杆菌胸膜肺炎血清学调查[J].中国动物检疫,1990(6)：21-22.
[7]王俊东,刘宗平.兽医临床诊断学[M].北京：中国农业出版社,2004：221-245.
[8]王建华,伍淳操.抗菌肽和蛋氨酸锌对断奶仔猪部分血液常规指标的影响[J].贵州农业科学,2010,38(3)：127-130.
[9]王岩,王久伶.急性细菌性痢疾388例分析[J].西南国防医药,2009,19(12)：1206-1208.
[10]卿柳庭,袁宗辉.育成猪对猪链球菌的免疫应答[J].中国兽医学报,2002,(05)：47-50.
[11]陈琼,廖三赛,贺长青,等.抗仔猪断奶应激剂对断奶仔猪血清生化指标及血常规的影响[J].广西畜牧兽医,2008,24(3)：136-139.
[12]闫广运.不同肝脏疾病检测指标的特征性变化特点与分析[J].医学信息(下旬刊),2010,23(10)：115-116.
[13]马玉芳,林雪玲,池春梅,等.母猪喂服中药黄白痢散对哺乳仔猪血液的有关生理生化及免疫学参数的影响[J].福建农林大学学报(自然科学版),2008,37(3)：286-289.
[14]王蔚,陈代文,毛湘冰,等.饲粮中三聚氰胺的添加对育肥猪肾脏结构与功能的影响[J].动物营养学报,2011,23(9)：1584-1591.
[15]刘震,周树录,李炜如,等.缺氧缺血性脑病新生猪的肾脏损害[J].华西医科大学学报,1997,28(2)：201-203.
[16]黄少萍.重症肝炎并发低血糖23例临床分析[J].右江医学,2010,38(6)：749.
[17]韩惠瑛,马海利,张艳红,等.附红细胞体自然感染猪血液生化指标的检测[J].中国兽医杂志,2003,39(11)：20-22.
[18]邓发清.自然感染附红细胞体病猪血液生理生化指标的测定[J].中国兽医杂志,2007,43(11)：43-44.
[19]周定刚,郑维明,钟妮娜.催产时鳝卵磷酸酶活性的变化及其与排卵的关系[J].水生生物学报,1993,17(2)：145-150.
[20]圈华,李莉.青藏高原不同生长期牦牛15项指标的测定[J].动物医学进展,2011,32(8)：62-65.
[21]Wrenger S, Kahne T, Bohuon C, et al. Amino-terminal truncation of procalcitonin, a marker for systemic bacterial infections, by dipeptidyl peptidase IV (DP IV)[J]. FEBS Letters,2000,466(1): 155-159.
[22]Sankaran R T, Mattana J, Pollack S, et al. Laboratory Abnormalities in Patients With Bacterial Pneumonia[J]. Chest,1997,111(3):595-600.
[23]Muller, Becker, Kranzlin, et al. Disordered calcium homeostasis of sepsis:association with calcitonin precursors[J]. European Journal of Clinical Investigation,2000,30(9):823-831.
[24]刘慧琳,魏敏,刘涛.降钙素基因相关肽对肺泡Ⅱ型上皮细胞的保护作用[J].实用医学杂志,2011,27(4)：587-589.
[1]SIEGEL I, TIAN Liu-liu, NORBERT G. The red-cell immune system [J]. Lancet,1981,318(9): 556-559.
[2]郭峰,钱宝华,张乐之.现代红细胞免疫学[M].上海：第二军医大学出版社,2002,6.
[3]Siegel I, Gleicher N. Red cell immune adherence (RCIA):its application in cancer and autoimmune disease. Immunol Commun.1981; 10(4-5):433-449.
[4]郭峰,虞紫茜,赵中丰等.红细胞免疫功能的初步研究[J].中华医学杂志,1982,62(12)：715-716.
[5]胡金川.红细胞免疫及其临床应用研究进展[J].国际检验医学杂志,2008,29(7)：621-622.
[6]侯海锋,包永占,李茜,等.当归对鸡红细胞免疫功能的影响[J].中国兽医杂志,2009(4)：54-56.
[7]李晓卉,殷中琼.青刺果黄酮对鸡红细胞免疫及外周血淋巴细胞免疫功能的影响[J].中国兽医杂志,2009(3)：43-44.
[8]彭西,柏才敏,李敏,等.高硒对雏鸡红细胞免疫功能红细胞总数及血红蛋白含量的影响[J].中国兽医科学,2008,38(6)：535-539.
[9]崔恒敏,彭西,黎德兵,等.高锌对雏鹅红细胞免疫功能的影响[J].中国兽医学报,2004,24(6)：576-578.
[10]刘福堂,李艳飞,顾庆云.铝中毒对蛋鸡白细胞和红细胞免疫功能的影响[J].中国畜牧兽医,2007,34(10)：70-72.
[11]黎德兵,崔恒敏,赵翠燕.鸭疫里默杆菌病发病机理的研究Ⅳ.试验感染雏鸭红细胞免疫功能的变化[J].黑龙江畜牧兽医,2007(9)：61-62.
[12]周惠萍,郭定宗,贺建忠.硒中毒对猪红细胞免疫与自由基的影响[J].安徽农业科学,2010(5)：2376-2377,2380.
[13]李清艳,翟向和,吕建存,等.附红细胞体感染对猪红细胞免疫功能的影响[J].中国兽医科技,2004,34(4)：46-48.
[14]富艳玲,王德志,王晓旭,等.中药乳膏剂对乳房炎奶牛非特异性免疫和红细胞免疫功能的影响[J].中国兽医杂志,2008,44(7)：51-53.
[15]王丽,剡根强,王静梅.绵羊自然感染附红细胞体对绵羊红细胞的影响[J].石河子大学学报：自然科学版,2006,24(1)：108-111.
[16]屈雪琪,赵建增,梁智选,等.流式细胞术检测不同试验条件对猪T淋巴细胞亚群的影响[J].中国畜牧兽医,2011,38(4)：101-104.
[17]李铭,刘宝金.猪T淋巴细胞亚群的研究进展[J].国外兽医学：畜禽传染病,1998,18(4)：26-27.
[18]胡永明,赵明秋,陈立军,等.猪瘟病毒感染对猪外周血T淋巴细胞亚群及TNF-a和IFN-γ的影响[J].中国预防兽医学报,2011,33(2)：126-129.
[19]孙金福,史子学,郭焕成,等.猪瘟病毒感染猪外周血白细胞凋亡及CD4+和CD8+T淋巴细胞亚群的变化[J].中国兽医科学,2008,38(4)：342-345.
[20]李华,杨汉春.猪繁殖与呼吸综合征病毒感染仔猪淋巴细胞亚群的动态[J].中国免疫学杂志,2001,17(4)：208-211.
[21]顾有方.猪生殖-呼吸道综合征病毒感染猪淋巴细胞亚群的变化[J].国外兽医学：畜禽传染病,1998,18(3)：47-49.
[22]沈艳,华修国,崔立,等.猪链球菌2型两代表性分离株感染小型猪后对部分免疫指标的影响[J].畜牧兽医学报,2008,39(12)：1721-1730.
[23]徐鹏,杨桂连,刘孝刚,等.仔猪人工感染猪囊尾蚴后外周血中淋巴细胞亚群的动态变化研究[J].吉林农业大学学报,2007,29(4)：440-442.
[24]Williams NH. Stahly TS, Zimmerman DR. Effect of chronic immune system activation on the rate, efficiency and composition of growth and lysine needs of pigs fed from 6 to 27 kg[J]. J Anita Sci, 1997a,75:2463-2471.
[25]Williams NH. Stahly TS. Zimmerman DR. Effect of chronic immune system activation on body nitrogen retention, partial efficiency of lysine utilization and lysine needs of pigs[J]. J Anim Sci, 1997b,75:2472-2480.
[26]Kim HM, Han SB, Oh GT, et al. Stimulation of humoral and cell mediated immunity by polysaccharide from mushroom Phellinus linteus. International Journal of Immunopharmacology, 1996,18:295-303.
[27]季敬璋,彭颖,吕建新.牛膝多糖体外诱导人T细胞表达IFN-γ和IL-4蛋白的机制探讨.中国免疫学杂志,2003,19(9)：611-613.
[28]Selye H. A Syndrome produced by diverse nocuous agents[J]. J Neuropsychiatry Clin Neurosci, 1998,10(2):230-231.
[29]Mader T L. Environmental stress in confined beef cattle [J]. J Anim Sci,2003,81(14):110-119.
[30]Eckersall PD, Saini PK, McComb C. The acute phase response of acid soluble glycoprotein, a1-acid glycoprotein, ceruloplasmin, haptoglobin and C-reactive protein, in the pig [J]. Vet Immunol Immunopathol,1996,51(3-4):377-385.
[31]Heegaard PMH, Klausen J, Nielsen JP, et al. The porcine acute phase response to infection with Actinobacillus pleuropneumoniae. Haptoglobin, C-reactive protein, major acute phase protein and serum amyloid A protein are sensitive indicators of infection[J]. Comp Biochem Physiol B Biochem Mol Biol,1998,119(2):365-373.
[32]Lauritzen B, Lykkesfeldt J, Skaanild MT, et al. Putative biomarkers for evaluating antibiotic treatment:an experimental model of porcine Actinobacillus pleuropneumoniae infection [J]. Res Vet Sci,2003,74(3):261-270.
[33]Hulten C, Johansson E, Fossum C, et al. Interleukin-6, serum amyloid A and haptoglobin as marker of treaqtment efficacy in pigs experimentally infected with Actinobacillus pleuropneumoniae [J]. Vet Microbiol,2003,95(1-2):75-89.
[34]Fossum C, Wattrang E, Fuxler L, et al. Evaluation of various cytokines (IL-6, IFN-a, IFN-g, TNF-a) as markers for acute bacterial infection in swine-a possible role for serum innterleukin-6[J]. Veterinary Immunology and Immunopathology,1998,64(2):161-172.
[35]彭西.日粮硒对雏鸡免疫功能影响的机理研究[D].四川农业大学博士论文,2009.
[36]彭西,崔赞,杨帆,等.日粮高硒对雏鸡外周血T淋巴细胞增殖功能和亚群的影响[J].畜牧兽医学报,2009,40(2)：256-260.
[37]周武,汪德清.红细胞功能检测方法的研究进展[J].中国输血杂志,2010,23(8)：648-650.
[38]薛拥志,王爱华,利凯.红细胞免疫研究进展[J].安徽农业科学,2007,35(12)：3558-3560,3664.
[39]刘景田,党小军.红细胞作为免疫细胞的事实及意义[J].深圳中西医结合杂志,2002,12(1)：11.
[40]王彩虹.红细胞免疫调控研究进展[J].中国兽医杂志,2002,38(2)：28-31.
[41]KIDD M T. Zinc metabolism with special reference to its role in immunity[J]. World Poult Sci J, 1996,52(2)：309-324.
[42]盛永杰,陈维多.硒对奶牛红细胞谷胱甘肽过氧化物酶活力的浓度依赖性研究[J].黑龙江畜牧兽医,2001,44(8)：8-9.
[43]温立斌,何孔旺,杨汉春,等.类圆环病毒因子P1感染对猪外周血T淋巴细胞含量和增殖活性的影响[J].华北农学报,2009,24(B08)：1-4.
[44]张佩君.猪外周血单个核细胞和植物血凝素对猪淋转试验的影响[J].陕西教育学院学报,2007,23(1)：88-90.
[45]杜念兴.兽医免疫学[M].北京：中国农业出版社,2002.
[46]Crawley A, Wilkie B N. Porcine Ig isotypes:function and molecular characteristics[J]. Vaccine, 2003,21(21-22):2911-2922.
[47]Brandtzaeg P. Molecular and cellular aspects of the secretory immunoglobulin system[J]. APMIS, 1995,103(1):1-19.
[48]Macpherson A J, Mcco YKD, Johansen FE, et al. The immune geography of IgA induction and function [J]. Mucosal Immunology,2008,1:11-22.
[49]周建.支气管肺炎患儿治疗前后血清IGF-Ⅱ、Hs-CRP和血液SIgA水平检测的临床意义[J].淮海医药,2011；29(4)：311-312.
[50]赵武述.免疫平衡研究及其临床意义[M].北京：科学出版社,2005：90.
[51]Malle E, De Beer F C. Human serum amyloid A (SAA) protein:a prominent acute-phase reactant for clinical practice [J]. Eur J Clin In-vest,1996,26(6):427-435.
[52]Yip T, Chan J, Cho W, et al. Protein chip array profiling analysis in patients with severe acute respirator syndrome identified serum amyloid A protein as a biomarker potentially useful in monitoring the extent of pneumonia[J]. Clin Chem,2005,51(1):47-55.
[53]Kokubun M, Imafuku Y, Okada M, et al. Serum amyloid A (SAA) concentration varies among rheumatoid arthritis patients estimated by SAA/CRPratio [J]. Clin ChimicaActa,2005,360 (1-2):97-102.
[54]Volanakis J E. Human C- reactive protein: expression, structure, and function[J]. Mol Immunol, 2001,38(2/3):189-197.
[55]GB Toews, Cytokines and the lung[J]. Eur. Respir J,2001,18(34):3-17.
[56]Duh EJ, Maury WJ, Folks TM, et al. Tumor necrosis factor alpha activates human immunodeficiency virus type 1 through induction of nuclear factor binding to the NF-kappa B sites in the long terminal repeat[J]. Proc Natl Acad Sci,1989,86 (15):5974-5978.
[57]Lowenthal JW, Ballard DW, Bohnlein E, et al. Tumor necrosis factor alpha induces proteins that bind specifically to kappa B-like enhancer elements and regulate interleukin 2 receptor alpha-chain gene expression in primary human T lymphocytes[J]. Proc Natl Acad Sci,1989,86(7):2331-2335.
[58]Bussolino F, G Camussi, C Baglioni. Synthesis and release of platelet-activating factor by human vascular endothelial cells treated with tumor necrosis factor or interleukin 1 alpha [J]. J Biol Chem, 1988,263(24):11856-11861.
[59]Libby P, JM Ordovas, KR Auger, et al. Endotoxin and tumor necrosis factor induce interleukin-1 gene expression in adult human vascular endothelial cells[J]. Am J Pathol,1986,124(2):179-185.
[60]PP Nawroth, I Bank, D Handley, et al. Tumor necrosis factor/cachectin interacts with endothelial cell receptors to induce release of interleukin 1[J]. J Exp Med,1986,163(6):1363-1375.
[61]Molnar L, Berki T, Hussain A, et al. Detection of TNFalpha expression in the bone marrow and determination of TNFalpha production of peripheral blood mononuclear cells in myelodysplastic syndrome[J]. Pathol Oncol Res,2000,6(l):18-23.
[62]Maier SF, Watkins LR. Cytokines for psychologists:implications of bidirectional immune-to-brain communication for understanding behavior, mood, and cognition[J]. Psychol Rev 1998,105: 83-107.
[63]Dinarello C A. Biologic basis for interleukin-1 in disease[J]. Blood,1996,87(6):2095-2147.
[64]Marz W, Wieland H. HMG-CoA reeducates inhibition:antiinflammatory effects beyond lipid lowering[J]. Herz,2000,25(2):117-125.
[65]王光辉,陈传兵,王献武.支气管肺炎患儿治疗前后血清IGF、IL-I、IL-6、IL-8和hs-CRP检测的临床意义[J].放射免疫学杂志,2009,22(4)：337-338.
[66]陈侃侃.肺炎支原体肺炎患儿血清hs-CRP、IL-6和IL-8检测的临床意义[J].放射免疫学杂志,2011,24(3)：266-267.
[67]Dooms H, Kahn E, Knoechel B, et al. IL-2 induces a competitive survival advantage in T lymphocytes[J]. J Immunol,2004,172(10):5973-5979.
[68]李爱华,曹祥山.IL-2与CD4+CD5+调节性T细胞的相关研究进展[J].医学综述,2007,13(9)：652-653.
[69]Trojanowska M. Role of PDGF in fibrotic diseases and systemic sclerosis[J]. Rheumatology,2008, 47(5):2-4.
[70]谢元林,藏雄益.国外医学.生理、病理科学与临床分册.1998,3：283-285.
[71]Gulumian M, Borm P J, Vallyathan V, et al. Mechanistically identified suitable biomarkers of exposure, effect, and susceptibility for silicosis and coal-worker's pneumoconiosis:a comprehensive review [J]. J Toxicol Environ Health B Crit Rev,2006,9(5):357-395.
[72]何燕,邓国忠,高天.肺纤维化细胞因子机制研究进展[J].中华创伤杂志,2006,6(22)：477-480.
[73]姚武,王治明,王绵珍等.煤工尘肺患者血清细胞因子及氧化损伤水平研究[J].四川大学学报(医学版),2005,36(4)：510-512.
[74]Santana A, Saxena B, Nancy A, et al. Increased expression of TGF-B1 in bleomycin-induced pulmonary fibrosis[J]. Am J Respir Cell Mol Biol,1995,13(1):34-44.
[75]Nagahori T, Dohi M, Matsumoto K, et al. Interferon-γ Upregulates the c-Met/Hepatocyte Growth Factor Receptor Expression in Alveolar Epithelial Cells[J]. Am J Respir Cell Mol Biol,1999,21(4): 490-497.
[76]Bajwa EK, Ayas NT, Schulzer M, et al. Interferon-gammalb therapy in idiopathic pulmonary fibrosis:a metaanalysis. Chest,2005,128(1):203-206.
[1]孙存普,张建中,段绍瑾.自由基生物学导论[M].合肥：中国科学技术大学出版社,1999.
[2]赵祥文.自由基与严重感染[J].临床儿科杂志,1993,11(3)：147-149.
[3]Imai Y, Kuba K, Neely GG, et al. Identification of oxidative stress and Toll-like receptor 4 signaling as a key pathway of acute lung injury[J]. Cell,2008,133(2): 235-249.
[4]杨旭夫,彭发泉.我国猪胸膜肺炎嗜血杆菌感染症的确定和诊断[J].畜牧兽医学报,1990,(03)：243-245.
[5]陈如明,谢鸣星,李云峰,等.鲁豫地区驻军猪传染性胸膜肺炎血清学调查[J].中国畜禽传染病,1990,(4)：38-40.
[6]邬捷,姜永康.四川猪嗜血杆菌胸膜肺炎血清学调查[J].动物检疫,1990(6)：21-22.
[7]Vander Vliet A, Cross CE. Oxidants, nitrosants, and the lung[J]. Am J Med,2000, 109(5):398-421.
[8]Vukic Z. Gluteal gunshot wounds [J]. Mill Med,2000,165(3):237-239.
[9]Ilic N, Petricevic A, Tanfara S, et al. War injuries to the chest. Acta Chir Hung,1999, 38:43-47.
[10]李清艳,翟向和,钟秀会,附红细胞体自然感染病猪血浆NO、SOD和MDA的变化[J].动物医学进展,2005,26(2)：92-94.
[11]毕晋明,郑姗姗,张敏红,等.日粮中添加不同浓度吡啶甲酸铬对生长肥育猪氧化作用的影响[J].动物营养学报,2008,20(5)：561-566.
[12]赵晶,康世良.健康仔猪口服亚硒酸钠后血液硒浓度与抗氧化系统动态变化规律的研究[J].畜牧兽医学报,2003,34(6)：554-557.
[13]Abu-khader AA, Biho YY. Exposure of human meutrophils to oxygen redicals causes loss of deformability. lipid peroxidation, protein degradation, respiratory burst activation and loss of migration[J]. Clin Hemorheol Microcirculat,2002,27(1): 57-66.
[14]Alverdy A M. Efects of glutamine-supplemented diets on immunology of the gut[J]. Parenter Enteral Nutr,1990,14:109-113.
[15]李敏,崔伟,彭西,等.高铜对雏鸡脑组织抗氧化酶活性的影响[J].畜牧兽医学报,2010,41(2)：220-22.
[16]王金胜,郭春绒,张映.农业生物化学技术[M].太原：山西科学技术出版社,1997：105-110.
[17]莫简.自由基在生物体系中的作用：普通中间介质或非特异性调节剂[J].自由基生命科学进展,1999(7)：7-10.
[18]李华强,史源,潘捷,等.内源性一氧化氮在感染性肺炎病理过程中的作用[J].第三军医大学学报,1999,21(3)：206-208.
[19]龙建纲,王学敏,高宏翔,等.丙二醛对大鼠肝线粒体呼吸功能及相关脱氢酶活性影响[J].第二军医大学学报,2005,26(10)：1131-1135.
[1]Hu J, He X. Enhanced quantile normalization of microarray data to reduce loss of information in gene expression profiles[J], Biometrics,2007,63(1):50-59.
[2]Xia X, McClelland M, Wang Y. WebArray:an online platform for microarray data analysis[J], BMC bioinformatics,2005,6(1):306.
[3]Romualdi C, Vitulo N, Favero M D, et al. MIDAW:a web tool for statistical analysis of microarray data[J], Nucleic acids research,2005,33(Web Server Issue):W644.
[4]Saeed AI, Sharov V, White J, et al. TM4:a free, open-source system for microarray data management and analysis[J]. Biotechniques.2003,34(2):374.
[5]Troyanskaya O3 Cantor M, Sherlock G, et al. Missing value estimation methods for DNA microarrays[J], Bioinformatics,2001,17(6):520.
[6]Pirooznia M, Nagarajan V, Deng Y. GeneVenn-A web application for comparing gene lists using Venn diagrams[J]. Bioinformation.2007,1(10):420.
[7]Lee HK, Braynen W, Keshav K, et al. ErmineJ:tool for functional analysis of gene expression data sets[J]. BMC Bioinformatics.2005,6(1):269.
[8]Mootha VK, Lindgren CM, Eriksson KF, et al. PCG-1a-responsive genes involved in oxidative phosphorylation are coordinately downregulated in human diabetes[J]. Nature genetics.2003, 34(3):267-273.
[9]Bild A, Febbo PG. Application of a priori established gene sets to discover biologically important differential expression in microarray data[J]. Proceedings of the National Academy of Sciences. 2005,102(43):15278.
[10]顾祖光.基因芯片数据分析中的基因集合分析技术[D].河北农业科学,2008,12(008)：169-170.
[11]Dennis Jr G, Sherman BT, Hosack DA, et al. DAVID:database for annotation, visualization, and integrated discovery[J]. Genome Biol,2003,4(5):3.
[12]Aoki-Kinoshita K F, Kanehisa M. Gene annotation and pathway mapping in KEGG[J]. Methods in MOLECULAR BIOLOGY-CLIFTON THEN TOTOWA,2007,396:71.
[13]Subramanian A, Tamayo P, Mootha VK, et al. Gene set enrichment analysis:a knowledge-based approach for interpreting genome-wide expression profiles[J]. Proceedings of the National Academy of Sciences.2005,102(43):15545.
[14]Erkens T, Van Poucke M, Vandesompele J, et al. Development of a new set of reference genes for normalization of real-time RT-PCR data of porcine backfat and longissimus dorsi muscle, and evaluation with PPARGC1A[J]. BMC Biotechnol,2006,6:41-52.
[15]Simmons PT, Portier CJ. Toxicogenomics:the new frontier in risk analysis[J], Carcinogenesis, 2002,23(6):903.
[16]Geller SC, Gregg JP, Hagerman P, et al. Transformation and normalization of oligonucleotide microarray data[J]. Bioinformatics.2003,19(14):1817-1823.
[17]Quackenbush J. Microarray data normalization and transformation[J]. Nat Genet.2002,32 Suppl: 496-501.
[18]邱浪波.基因芯片表达数据分析相关问题研究[D].湖南长沙,国防科学技术大学,2007.
[19]王永煜,张幼怡.基因芯片数据分析与处理[J].生物化学与生物物理进展.2003,30(002)：321-323.
[20]伍亚舟,张彦琦,黄明辉,等.基因芯片表达数据的标准化策略研究[J].第三军医大学学报.2004,26(007)：594-597.
[21]谈效俊,张永新,钱敏,等.芯片数据标准化方法比较研究[J].生物化学与生物物理进展，2007,34(006)：625-633.
[22]Oba S, Sato MA, Takemasa I, et al. A Bayesian missing value estimation method for gene expression profile data[J]. Bioinformatics.2003,19(16):2088-2096.
[23]Ouyang M, Welsh WJ, Georgopoulos P. Gaussian mixture clustering and imputation of microarray data[J]. Bioinformatics.2004,20(6):917-923.
[24]Kim H, Golub GH, Park H. Missing value estimation for DNA microarray gene expression data: local least squares imputation[J]. Bioinformatics.2005,21(2):187-198.
[25]Yoon D, Lee EK, Park T. Robust imputation method for missing values in microarray data[J]. BMC Bioinformatics.2007,8 Suppl 2:S6.
[26]Kerr MK. Design considerations for efficient and effective microarray studies[J], Biometrics,2003, 59(4):822-828.
[27]Tanaka TS, Jaradat SA, Lim MK, et al. Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray[J]. Proceedings of the National Academy of Sciences of the United States of America.2000,97(16):9127.
[28]Yang IV, Chen E, Hasseman JP, et al. Within the fold:assessing differential expression measures and reproducibility in microarray assays[J]. Genome Biol.2002,3(11):1-0062.0012.
[29]Tusher VG, Tibshirani R, Chu G. Significance analysis of microarrays applied to the ionizing radiation response[J]. Proc Natl Acad Sci USA.2001,98(9):5116-5121.
[30]Wright GW, Simon RM. A random variance model for detection of differential gene expression in small microarray experiments [J]. Bioinformatics.2003,19(18):2448-2455.
[31]Baldi P, Long AD. A Bayesian framework for the analysis of microarray expression data: regularized t-test and statistical inferences of gene changes[J]. Bioinformatics.2001,17(6): 509-519.
[32]Long AD, Mangalam HJ, Chan BY, et al. Improved statistical inference from DNA microarray data using analysis of variance and a Bayesian statistical framework. Analysis of global gene expression in Escherichia coli K12[J]. J Biol Chem.2001,276(23):19937-19944.
[33]孙如平.结合基因组调控信息的基因芯片数据综合分析[D].复旦大学；2007.
[34]Heyer LJ, Kruglyak S, Yooseph S. Exploring expression data: identification and analysis of coexpressed genes [J]. Genome Res.1999,9(11):1106-1115.
[35]Tavazoie S, Hughes JD, Campbell MJ, et al. Systematic determination of genetic network architecture[J]. Nat Genet.1999,22(3):281-285.
[36]Tamayo P, Slonim D, Mesirov J, et al. Interpreting patterns of gene expression with self-organizing maps:methods and application to hematopoietic differentiation [J]. Proc Natl Acad Sci USA.1999, 96(6):2907-2912.
[37]Breiman L. Arcing classifiers[J]. Annals of statistics.1998,26(3):801-824.
[38]Seok J, Kaushal A, Davis RW, et al. Knowledge-based analysis of microarrays for the discovery of transcriptional regulation relationships[J]. BMC Bioinformatics.11 Suppl 1:S8.
[39]Winters-Hilt S, Yelundur A, McChesney C, et al. Support vector machine implementations for classification & clustering[J]. BMC Bioinformatics.2006,7 Suppl 2:S4.
[40]蔡立军.基因分类及基因表达数据分析方法的研究[D].湖南大学；2007.
[41]李明洲.脂肪型和瘦肉型猪肌肉生长和脂肪沉积相关基因的差异表达分析和分子网络构建[D].四川农业大学,2008.
[42]Rhee S Y, Wood V, Dolinski K, et al. Use and misuse of the gene ontology annotations[J]. Nature Reviews Genetics,2008,9(7):509-515.
[43]Hong E L, Balakrishnan R, Dong Q, et al. Gene Ontology annotations at SGD:new data sources and annotation methods[J]. Nucleic acids research,2008,36(Database issue):D577.
[44]Camon E, Barrell D, Lee V, et al. The Gene Ontology Annotation (GOA) Database-an integrated resource of GO annotations to the UniProt Knowledgebase[J]. In Silico Biology,2004,4(1):5-6.
[45]Ogata H, Goto S, Fujibuchi W, et al. Computation with the KEGG pathway database[J]. Biosystems,1998,47(1-2):119-128.
[46]Arakawa K, Kono N, Yamada Y, et al. KEGG-based pathway visualization tool for complex omics data[J]. In Silico Biology,2005,5(4):419-423.
[47]Paterlini M, Zakharenko SS, Lai WS, et al. Transcriptional and behavioral interaction between 22q11.2 orthologs modulates schizophrenia-related phenotypes in mice[J]. Nature neuroscience. 2005,8(11):1586-1594.
[48]方焯.基于生物知识的生物芯片表达谱数据分析研究[D].湖北武汉,华中科技大学,2006.
[49]Dopazo J. Functional interpretation of microarray experiments[J]. OMICS:A Journal of Integrative Biology,2006,10(3):398-410.
[50]Shaw MH, Reimer T, Kim YG, et al. NOD-like receptors (NLRs):bona fide intracellular microbial sensors [J]. Curr Opin Immunol,2008,20:377-82.
[51]Kanneganti TD, Lamkanfi M, Nunez G. Intracellular NOD-like receptors in host defense and disease[J]. Immunity,2007,27:549-59.
[52]Franchi L, Warner N, Viani K, et al. Function of Nod-like receptors in microbial recognition and host defense[J]. Immunol Rev,2009,227:106-28.
[53]Tattoli I, Travassos LH, Carneiro LA, et al. The Nodosome:Nod1 and Nod2 control bacterial infections and inflammation[J]. Semin Immunopathol 29:289-301.
[54]Reardon C, Mak TW. cIAP proteins:keystones in NOD receptor signal transduction[J]. Immunity 30:755-6.
[55]Fritz JH, Ferrero RL, Philpott DJ, et al. Nod-like proteins in immunity, inflammation and disease[J]. Nat Immunol,2006,7:1250-7.
[56]Rietdijk ST, Burwell T, Bertin J, et al. Sensing intracellular pathogens-NOD-like receptors[J]. Curr Opin Pharmacol,2008,2008,8:261-6.
[57]Franchi L, Eigenbrod T, Munoz-Planillo R, et al. The inflammasome:a caspase-1-activation platform that regulates immune responses and disease pathogenesis[J]. Nat Immunol,2009, 10:241-7.
[58]Martinon F, Gaide O, Petrilli V, et al. NALP inflammasomes:a central role in innate immunity[J]. Semin Immunopathol,2007,29:213-29.
[59]Martinon F, Tschopp J. Inflammatory caspases:linking an intracellular innate immune system to autoinflammatory diseases[J]. Cell,2004,117:561-74.
[60]Benko S, Philpott DJ, Girardin SE. The microbial and danger signals that activate Nod-like receptors[J]. Cytokine,2008,43:368-73.
[61]Kawakami T, Galli SJ. Regulation of mast-cell and basophil function and survival by IgE[J]. Nat Rev Immunol,2002,2:773-86.
[62]Siraganian RP. Mast cell signal transduction from the high-affinity IgE receptor[J]. Curr Opin Immunol,2003,15:639-46.
[63]Turner H, Kinet JP. Signalling through the high-affinity IgE receptor Fc epsilonRI[J]. Nature,1999, 402:B24-30.
[64]Gilfillan AM, Tkaczyk C. Integrated signalling pathways for mast-cell activation[J]. Nat Rev Immunol,2006,6:218-30.
[65]Nadler MJ, Matthews SA, Turner H, et al. Signal transduction by the high-affinity immunoglobulin E receptor Fc epsilon RI:coupling form to function[J]. Adv Immunol,2000,76:325-55.
[66]Steffen B, Muller-Tidow C, Schwable J, et al. The molecular pathogenesis of acute myeloid leukemia[J]. Crit Rev Oncol Hematol,2005,56:195-221.
[67]Tenen DG. Disruption of differentiation in human cancer: AML shows the way[J]. Nat Rev Cancer, 2003,3:89-101.
[68]Martelli AM, Nyakern M, Tabellini G, et al. Phosphoinositide 3-kinase/Akt signaling pathway and its therapeutical implications for human acute myeloid leukemia[J]. Leukemia,2006,20:911-28.
[69]Lennartsson J, Jelacic T, Linnekin D, et al. Normal and oncogenic forms of the receptor tyrosine kinase kit[J]. Stem Cells,2005,23:16-43.
[70]Lorsbach RB, Downing JR. The role of the AML1 transcription factor in leukemogenesis[J]. Int J Hematol,2001,74:258-65.
[71]Mizuki M, Schwable J, Steur C, et al. Suppression of myeloid transcription factors and induction of STAT response genes by AML-specific Flt3 mutations[J]. Blood,2003,101:3164-73.
[72]Nebreda AR, Ferby I. Regulation of the meiotic cell cycle in oocytes[J]. Curr Opin Cell Biol,2000, 12:666-75.
[73]Andersen CB, Sakaue H, Nedachi T, et al. Protein kinase B/Akt is essential for the insulin-but not progesterone-stimulated resumption of meiosis in Xenopus oocytes[J]. Biochem J,2003, 369:227-38.
[74]Abrieu A, Doree M, Fisher D. The interplay between cyclin-B-Cdc2 kinase (MPF) and MAP kinase during maturation of oocytes[J]. J Cell Sci,2001,114:257-67.
[75]Ghayee HK, Auchus RJ. Basic concepts and recent developments in human steroid hormone biosynthesis[J]. Rev Endocr Metab Disord,2007,8:289-300.
[76]Holmes MC, Seck1 JR. The role of 1 lbeta-hydroxysteroid dehydrogenases in the brain[J]. Mol Cell Endocrinol,2006,248:9-14.
[77]Thomas JL, Duax WL, Addlagatta A, et al. Structure/function relationships responsible for coenzyme specificity and the isomerase activity of human type 13 beta-hydroxysteroid dehydrogenase/isomerase[J]. J Biol Chem,2003,278:35483-90.
[78]Tomlinson JW, Stewart PM. Cortisol metabolism and the role of 1 lbeta-hydroxysteroid dehydrogenase[J]. Best Pract Res Clin Endocrinol Metab,2001,15:61-78.
[79]Gual P, Le Marchand-Brustel Y, Tanti JF. Positive and negative regulation of insulin signaling through IRS-1 phosphorylation[J]. Biochimie,2005,87:99-109.
[80]Kaneto H, Matsuoka TA, Nakatani Y, et al. Oxidative stress, ER stress, and the JNK pathway in type 2 diabetes[J]. J Mol Med,2005,83:429-39.
[81]Sakai K, Matsumoto K, Nishikawa T, et al. Mitochondrial reactive oxygen species reduce insulin secretion by pancreatic beta-cells[J]. Biochem Biophys Res Commun,2003,300:216-22.
[82]Robertson RP. Chronic oxidative stress as a central mechanism for glucose toxicity in pancreatic islet beta cells in diabetes[J]. J Biol Chem,2004,279:42351-4.
[83]Markus C. Wahl, Cindy L. Will, Reinhard Lu" hrmann. The spliceosome:design principles of a dynamic RNP machine[J]. Cell,136:701-18.
[84]Morris DP, Greenleaf AL. The splicing factor, Prp40, binds the phosphorylated carboxyl-terminal domain of RNA polymerase II[J]. J Biol Chem,2000,275:39935-43.
[85]Chen YI, Moore RE, Ge HY, et al. Proteomic analysis of in vivo-assembled pre-mRNA splicing complexes expands the catalog of participating factors[J]. Nucleic Acids Res,2007,35:3928-44.
[86]Ritchie DB, Schellenberg MJ, Macmillan AM. Spliceosome structure:Piece by piece[J]. Biochim BiophysActa,2009,1789:624-633.
[87]Levy C, Khaled M, Fisher DE. MITF:master regulator of melanocyte development and melanoma oncogene[J]. Trends Mol Med,2006,12:406-14.
[88]Tsao H, Goel V, Wu H, et al. Genetic interaction between NRAS and BRAF mutations and PTEN/MMAC1 inactivation in melanoma[J]. J Invest Dermatol,2004,122:337-41.
[89]Gruss C, Herlyn M. Role of cadherins and matrixins in melanoma[J]. Curr Opin Oncol,2001, 13:117-23.
[90]Lazar-Molnar E, Hegyesi H, Toth S,et al. Autocrine and paracrine regulation by cytokines and growth factors in melanoma[J]. Cytokine,2000,12:547-54.
[91]Liu T, Clark RK, McDonnell PC, et al. Tumor necrosis factor-alpha expression in ischemic neurons[J]. Stroke,1994,25:1481-8.
[92]Goukassian DA, Qin G, Dolan C, et al. Tumor necrosis factor-alpha receptor p75 is required in ischemia-induced neovascularization[J]. Circulation,2007,115:752-62.
[93]Yoshida S, Ono M, Shono T, Izumi H, et al. Involvement of interleukin-8, vascular endothelial growth factor, and basic fibroblast growth factor in tumor necrosis factor alpha-dependent angiogenesis[J]. Mol Cell Biol,1997,17:4015-23.
[94]Limb GA, Chignell AH, Green W, et al. Distribution of TNF alpha and its reactive vascular adhesion molecules in fibrovascular membranes of proliferative diabetic retinopathy[J]. Br J Ophthalmol,1996,80:168-73.
[95]NM Cirino, JR Panuska, A Villani, et al. Restricted replication of respiratory syncytial virus in human alveolar macrophages[J]. J Gen Virol,1993,74 (8):1527-1537.
[96]MT Heise, HWT Virgin. The T-cell-independent role of gamma interferon and tumor necrosis factor alpha in macrophage activation during murine cytomegalovirus and herpes simplex virus infections[J]. J Virol,1995,69(2):904-909.
[97]GH Wong, DV Goeddel. Tumour necrosis factors alpha and beta inhibit virus replication and synergize with interferons[J]. Nature,1986,323(6091):819-822.
[98]GB Toews. Cytokines and the lung[J]. Eur Respir J Suppl,2001,34:3-17.
[99]Duh EJ, WJ Maury, TM Folks, et al. Tumor necrosis factor alpha activates human immunodeficiency virus type 1 through induction of nuclear factor binding to the NF-kappa B sites in the long terminal repeat[J]. Proc Natl Acad Sci USA,1989,86:5974-5978.
[100]Kronke M, S Schutze, P Scheurich, et al. TNF signal transduction and TNF-responsive genes[J]. Immunol Ser,1992,56:189-216.
[101]Lowenthal JW, DW Ballard, E Bohnlein, et al. Tumor necrosis factor alpha induces proteins that bind specifically to kappa B-like enhancer elements and regulate interleukin 2 receptor alpha-chain gene expression in primary human T lymphocytes[J]. Proc Natl Acad Sci USA,1989, 86:2331-2335.
[102]Osborn L, S Kunkel, GJ Nabel. Tumor necrosis factor alpha and interleukin 1 stimulate the human immunodeficiency virus enhancer by activation of the nuclear factor kappa B[J]. Proc Natl Acad Sci USA,1989,86:2336-2340.
[103]Schutze S, K Potthoff, T Machleidt, et al.TNF activates NF-kappa B by phosphatidylcholine-specific phospholipase C-induced "acidic" sphingomyelin breakdown[J]. Cell, 1992,71:765-776.
[104]Bussolino F, G Camussi, C Baglioni. Synthesis and release of platelet-activating factor by human vascular endothelial cells treated with tumor necrosis factor or interleukin 1 alpha[J]. J Biol Chem, 1988,263:11856-11861.
[105]Libby P, JM Ordovas, KR Auger, et al. Endotoxin and tumor necrosis factor induce interleukin-1 gene expression in adult human vascular endothelial cells[J]. Am J Pathol,1986,124:179-185.
[106]Nawroth PP, I Bank, D Handley, et al.Tumor necrosis factor/cachectin interacts with endothelial cell receptors to induce release of interleukin 1[J]. J Exp Med,1986,163:1363-1375.
[107]Pober JS, M Gimbrone, LA Lapierre, et al. Overlapping patterns of activation of human endothelial cells by interleukin 1, tumor necrosis factor, and immuneinterferon[J]. J Immunol, 1986,137:1893-1896.
[108]Wajant H, Pfizenmaier K, Scheurich P. Tumor necrosis factor signaling[J]. Cell Death Differ, 2003,10:45-65.
[109]Molnar L, Berki T, Hussain A, et al. Detection of TNF alpha expression in the bone marrow and determination of TNFalpha production of peripheral blood mononuclear cells in myelodysplastic syndrome[J]. Pathol Oncol Res,2000,6:18-23.
[110]Chrysant SG. The path physiologic role of the brain Renin-Angiotensin system in stroke protection:clinical implications[J]. J Clin Hypertens(Greenwich),2007,9(6):454-459.
[111]Weiner OD. Regulation of cell polarity during eukaryotic chemotaxis:the chemotactic compass. Curr Opin Cell Biol,2002,14:196-202.
[112]Vanhaesebroeck B, Jones GE, Allen WE, et al. Distinct PI(3) Ks mediate mitogenic signalling and cell migration in macrophages. Nat Cell Biol,1999,1:69-71.
[113]Keqiang Chen, Pablo Iribarren, Wanghua Gong, et al. The Essential Role of Phosphoinositide 3-Kinases(PI3Ks)in Regulating Pro-Inflammatory Responses and the Progression of Cancer[J]. Cellular & Molecular Immunology,2005,2(4):241-252.
[114]Schmidt R, Redecke V, Breitfeld Y, et al. EMMPRIN (CD 147)is a central activator of extracellular matrix degradation by Chlamydia pneumoniae-infected monocytes. Implications for plaque rupture[J]. Thromb Haemost,2006,95(1):151-158.
[115]L Stephens, C Ellson, P Hawkins. Roles of PI3Ks in leukocytes chemotaxis and phagocytosis, Curr. Opin[J]. Cell Biol,14 (2002):203-213.
[116]MP Wymann, M Zvelebil, M Laffargue. Phosphoinositide 3-kinase signalling-which way to target?[J]. Trends Pharmacol Sci,2003,24:366-376.
[117]T Sasaki, J Irie-Sasaki, RG Jones, et al. Function of PI3Ky in thymocyte development, T cell activation, and neutrophil migration[J]. Science,2000,287:1040-1046.
[118]E Hirsch, VL Katanaev, C Garlanda, et al.Central role for G protein-coupled phosphoinositide 3-kinase y in inflammation[J]. Science,2000,287:1049-1053.
[119]Z Li, H Jiang, W Xie, et al. Roles of PLC-β2 and -β3 and PI3Ky in chemoattractant- mediated signal transduction[J]. Science,2000,287:1046-1049.
[120]MA Crackower, GY Oudit, I Kozieradzki, et al. Regulation of myocardial contractibility and cell size by distinct PI3K-PTEN signaling pathways[J]. Cell,2002,:737-749.
[121]Brock C, Schaefer M, Reusch HP, et al. (Jan). Roles of G beta gamma in membrane recruitment and activation of p110 gamma/p101 phosphoinositide 3-kinase gamma[J]. J Cell Biol,2003,160, (1):89-99.
[122]"Entrez Gene:PIK3R5 phosphoinositide-3-kinase, regulatory subunit 5, p101". http://www. ncbi. nlm. nih. gov/sites/entrez?Db=gene&Cmd=ShowDetailView & TermToSearch=23533.
[123]Voigt P, Brock C, Nurnberg B, et al. Assigning functional domains within the p101 regulatory subunit of phosphoinositide 3-kinase gamma[J]. J Biol Chem,2005,280(6):5121-7.
[124]Bassi R, Heads R, Marber MS, Clark JE. Targeting p38-MAPK in the ischaemic heart:kill or cure?[J]. Curr Opin Pharmacol,2008,8:141-6.
[125]Irving EA, Bamford M. Role of mitogen- and stress-activated kinases in ischemic injury[J]. J Cereb Blood Flow Metab,2002,22:631-47.
[126]Nozaki K, Nishimura M, Hashimoto N. Mitogen-activated protein kinases and cerebral ischemia[J]. Mol NeuroBiol,2001,23:1-19.
[127]Ravingerova T, Barancik M, Strniskova M. Mitogen-activated protein kinases:a new therapeutic target in cardiac pathology[J]. Mol Cell Biochem,2003,247:127-38.
[128]Bodil Gesslein, Gisela Hakansson, Ronald Carpio, et al. Mitogen-activated protein kinases in the porcine retinal arteries and neuroretina following retinal ischemia-reperfusion[J]. Mol Vis,2010, 16:392-407.
[129]Lai EW, Toledo-Pereyra LH, Walsh J, et al. The role of MAP kinases in trauma and ischemia-reperfusion[J]. J Invest Surg,2004,17:45-53.
[130]Roux PP, Blenis J. ERK and p38 MAPK-activated protein kinases:a family of protein kinases with diverse biological functions[J]. Microbiol Mol Biol Rev,2004,68:320-44.
[131]Kubota Y, O'Grady P, Saito H, et al. Oncogenic Ras abrogates MEK SUMOylation that suppresses the ERK pathway and cell transformation[J]. Nat Cell Biol,2011,13(3):282-91.
[132]Daughaday WH, Rotwein P. Insulin-like growth factor Ⅰ and Ⅱ. Peptide, messenger ribonucleic acid and gene structures, serum, and tissue concentrations[J]. Endocr Rev,1989,10:68-91.
[133]Christoforidis A, Maniadaki I, Stanhope R. Growth hormone/insulin-like growth factor-1 axis during puberty[J]. Pediatr Endocrinol Rev,2005,3:5-10.
[134]Dunshea FR, Barneveld RJ. Colostrum protein isolate enhances gut development, growth performance and plasma IGF-I and II in neonatal pigs Asia Pac[J]. J Clin Nutr,12:40.
[135]Boutinaud M, Shand JH, Park MA, et al. A quantitative RT-PCR study of the mRNA expression profile of the IGF axis during mammary gland development[J]. J Mol Endocrinol,33:195-207.
[136]Marz W, Wieland H. HMG-CoA reeducates inhibition:antiinflammatory effects beyond lipid lowering[J]. Herz,2000,25(2):117-125.
[137]Nakanishi K, Yoshimoto T, Tsutsui H, et al. Interleukin-18 regulates both Thl and Th2 responses[J]. Annu Rev Immunol,2001,19:423-474.
[138]Wolff B, Burns AR, Middleton J, et al. Endothelial cell "memory" of inflammatory stimulation: human venular endothelial cells store interleukin 8 in Weibel-Palade bodies[J]. J Exp Med,188(9): 1757-62.
[139]Utgaard JO, Jahnsen FL, Bakka A, et al. Rapid secretion of prestored interleukin 8 from Weibel-Palade bodies of microvascular endothelial cells[J]. J Exp Med,188(9):1751-6.
[140]Hoshino T, Wiltrout RH, Young HA. IL-18 is a potent coinducer of IL-13 in NK and T cells:A new potential role for IL-18 in modulating the immune response[J]. J Immunol,1999,162: 5070-5077.
[141]Yoshimoto T, Tsutsui H, Tominaga K, et al. IL-18, although antiallergic when administered with IL-12, stimulates IL-4 and histamine release by basophils[J]. Proc Natl Acad Sci USA,1999,96: 13962-13966.
[142]Yoshimoto T, Mizutani H, Tsutsui H, et al. IL-18 induction of lgE: Dependence on CD4+ T cells, IL-4 and STAT6[J]. Nat Immunol,2000,1,132-137.
[143]Yoshimoto T, Min B, Sugimoto T, et al. Nonredundant roles for CD Id-restricted natural killer T cells and conventional CD4+ T cells in the induction of immunoglobulin E antibodies in response to interleukin 18 treatment of mice. J Exp Med,2003,197:997-1005.
[144]Carr MW, Roth SJ, Luther E, et al. Monocyte chemoattractant protein 1 acts as a T-lymphocyte chemoattractant[J]. Proc Natl Acad Sci USA,1994,91(9):3652-6.
[145]Xu LL, Warren MK, Rose WL, et al. Human recombinant monocyte chemotactic protein and other C-C chemokines bind and induce directional migration of dendritic cells in vitro[J]. J Leukoc Biol,1996,60 (3):365-71.
[146]Peelman LJ, P Chardon, M Nunes, et al. The BAT1 gene in the MHC encodes an evolutionarily conserved putative nuclear RNA helicase of the DEAD family[J]. Genomics 1995,26:210-218.
[147]Spies T, G Blanck, M Bresnahan, et al. A new cluster of genes within the human major histocompatibility complex[J]. Science,1989,243:214-217.
[148]Spies T, M Bresnahan, JL Strominger.Human major histocompatibility complex contains a minimum of 19 genes between the complement cluster and HLA-B[J]. Proc Natl Acad Sci USA, 1989,86:8955-8958.
[149]Von Bubnoff D, Novak N, Kraft S, et al. The central role of FcepsilonRI in allergy[J]. Clin Exp Dermatol,28(2):184-7.
[150]Gounni A, Lamkhioued B, Ochiai K, et al. High-affinity IgE receptor on eosinophils is involved in defence against parasites[J]. Nature,367 (6459):183-6.
[151]Kisseleva T, Bhattacharya S, Braunstein, J, et al. Signaling through the JAK/STAT pathway, recent advances and future changes[J]. Gene,2002,285(1-2),1-24.
[152]Jakob Hedegaard, Kerstin Skovgaard, Shila Mortensen,et al. Molecular characterisation of the early response in pigs to experimental infection with Actinobacillus pleuropneumoniae using cDNA microarrays. BMC Genomics,2007,49:11.
[153]Cantrell DA, Smith KA. The interleukin-2 T-cell system:a new cell growth model[J]. Science, 1984,224(4655):1312-1316.
[154]Smith KA. Interleukin-2:inception, impact, and implications[J]. Science,1988,240, (4856): 1169-1176.
[155]金伯泉.医学免疫学[M].北京：人民卫生出版社,第5版,2008：60-65,105-107.
[156]刘艳芳,汤建民.白细胞介素2局部治疗恶性胸腔积液的临床观察[J].中国医药指南,2011,9(9)：54-55.
[157]李丽,张淑霞,韩文宁,等.白细胞介素2、8检测在婴幼儿重症支原体肺炎中的临床价值[J].中国生育健康杂志,2010,21(2)：101-103.
[158]Dooms H, Kahn E, Knoechel B, et al. IL-2 induces a competitive survival advantage in T lymphocytes[J]. J Immunol,2004,172(10):5973-5979.
[159]杜德伟,白宪光,冯志华,等.白细胞介素2提高HBV基因疫苗诱导的体液免疫应答[J].实用肝脏病杂志,2003(2)：78-79.
[160]Waldmann TA. The biology of interleukin-2 and interleukin-15:implications for cancer therapy and vaccine design[J]. Nature Rev Immun,2006,6 (8):595-601.
[161]Waldmann TA, Tagaya Y. The multifaceted regulation of interleukin-15 expression and the role of this cytokine in NK cell differentiation and host response to intracellular pathogens[J]. Annu Rev Immunol,1999,17:19-49.
[162]Wolf SF, Temple PA, Kobayashi M, et al. Clong of cDNA for natural killer cell stimulatory factor, a heterodimeric cytokine with multiple biologic effect of T and natural killer cells[J]. J Immunol,1991,146(9):3074-3081.
[163]Sten As, Podlaski FJ, Hulmes JD, et al. Purincation to homogeneity and partial characterization of cytotoxic lymphocyte maturalion factor from human B-lymphoblastoid cells[J]. Proc Natl Acad sci USA,1990,87(17):6808-6812.
[164]Podlaski FJ, Nanduri VB, Hulmes JD, et al. Molecular characterization of interleukin 12[J]. Arch Biochem Biophys,1992,294(1):230-237.
[165]叶松道,郑晓群,杨锦红,等.巨细胞病毒肝炎患儿IL-12 p403’非翻译区基因多态性研究[J].医学研究杂志,2010,39(12)：92-96.
[166]Oppmann B, Lesley R, Blom B,et al. Novel p19 protein engages IL-12 p40 to form a cytokine, IL-23, with biological activities similar as well as distinct from IL-12[J]. Immunity,13(5):715-25.
[167]袁远英,方强,孙新,等.猪白细胞介素12 p35、p40亚基基因的克隆和序列分析[J].蚌埠医学院学报,2008,33(6)：644-647.
[168]杨瑛,汤绍涛,曹国庆,等.L一12 P35,P40和IFN-γ在胆道闭锁小鼠模型肝脏组织中的表达[J].临床小儿外科杂志,2008,7(6)：26-29.
[169]黄建安,顾冬梅,庄羽美,等.CD40分子在肺癌中的表达及其临床意义[J].江苏医药,2003,29(9)：645-647.
[170]Van Kooten C, Bancherean J. Functions of CD40 on B cells, dendritic cells and other cells[J]. Curropin Immunol.1997,9:330-341.
[171]王淑娟,陈明,李鸿佳,等.脂多糖诱导小鼠肺损伤模型巨噬细胞活化及共刺激分子CD40上调研究[J].中华微生物学和免疫学杂志,2007,27(12)：1076-1080.
[172]Sime PJ, O'Reilly KM. Fibro sis of the lung and other tissues:new concepts in pathogenesis and treatment[J]. Clin Immunol,2001,99(3):308-319.
[173]吴彬,但伶.CD40-CD40L与急性肺损伤[J].重庆医科大学学报,2007：114-115,118.
[174]Suttles J, Evans M, Miller RW, et al. T cell rescue of monocytes from apoptosis:ro le of the CD40-CD40L interaction and requirement for CD40-mediated induction of protein tyrosine kinase activity [J]. J Leukoc Biol,1996,60(5):651-657.
[175]Phipps RP, Stein SH, Roper RL. A new view of pro staglandin E regulation of the immune response[J]. Immunol Today,1991,12(10):349-352.
[176]Zhang-Hoover J, Sutton A, Stein-Streilein J. CD40/CD40 ligand interactions are critical for elicitation of autoimmune-mediated fibrosis in the lung[J]. J Immunol,2001,166(5):3556-3563.
[177]Ishida TK, Tojo T, Aoki T, et al. TRAF5, a novel tumor necrosis factor receptor-associated factor family protein, mediates CD40 signaling[J]. Proc Natl Acad Sci USA,1996,93(18):9437-42.
[178]Clark EA. CD40:a cytokine receptor in search of a ligand[J]. Tissue Antigens,1991,36(1):33-6.
[179]Banchereau J, Bazan F, Blanchard D, et al. The CD40 antigen and its ligand[J]. Annu Rev Immunol,1994,12:881-922.
[180]Cheng G, Schoenberger SP. CD40 signaling and autoimmunity[J]. Curr Dir Autoimmun,2002,5: 51-61.
[181]Dallman C, Johnson PW, Packham G. Differential regulation of cell survival by CD40[J]. Apoptosis,2003,8(1):45-53.
[182]O'Sullivan B, Thomas R. Recent advances on the role of CD40 and dendritic cells in immunity and tolerance[J]. Curr Opin Hematol,2004,10(4):272-8.
[183]Abbas, Lichtman AH. Ch3 Antigen capture and presentation to lymphocytes [J]. Basic Immunology Functions and disorders of the immune system (3rd ed),2009,978(1):4160-4688.
[184]Klein J, Figueroa F. Evolution of the major histocompatibility complex[J]. Crit Rev Immunol, 1986,6(4):295-386.
[185]Kelly PT, Mackinnon RL 2nd, Dietz RV, et al. Postsynaptic IP3 receptor- mediated Ca2+ release modulates synaptic transmission in hippocampal neurons. Brain Res Mol Brain Res,2005, 135:232-48.
[186]Munoz-Chapuli R, Quesada AR, Angel Medina M. Angiogenesis and signal transduction in endothelial cells. Cell Mol Life Sci,2004,61:2224-43.
[187]Zachary I. VEGF signalling: integration and multi-tasking in endothelial cell biology. Biochem Soc Trans,2003,31:1171-7.
[188]Takahashi H, Shibuya M. The vascular endothelial growth factor (VEGF)/VEGF receptor system and its role under physiological and pathological conditions. Clin Sci (Lond),2005,109:227-41.
[189]Hoeben A, Landuyt B, Highley MS, et al. Vascular endothelial growth factor and angiogenesis. Pharmacol Rev,2004,56:549-80.
[190]Matsumoto T, Claesson-Welsh L.VEGF-receptor signal transduction[J]. Trends Biochem Sci, 2003,28:488-94.
[191]Chuaqui RF, Bonner RF, Best CJ, et al. Post-analysis follow-up and validation of microarray experiments[J]. Nat Genet,2002,32:509-534.
[192]Brazma A, Hingamp P, Quackenbush J, et al. Minimum information about a microarray experiment (MIAME)-toward standards for microarray data[J], Nature genetics,2001,29(4): 365-371.
[193]李瑶.基因芯片数据分析与处理[M].北京：化学工业出版社.2006：133-142.
[194]Rockett JC, Hellmann GM. Confirming microarray data--is it really necessary?[J]. Genomics, 2004,83(4):541-549.
[195]Qin LX, Beyer RP, Hudson FN, et al. Evaluation of methods for oligonucleotide array data via quantitative real-time PCR[J]. BMC Bioinformatics,2006,7:23-31.
[196]Etienne W, Meyer MH, Peppers J, et al. Comparison of mRNA gene expression by RT-PCR and DNA microarray [J]. Biotechniques,2004,36(4):618-620,622,624-616.
[197]Dallas PB, Gottardo NG, Firth MJ, et al. Gene expression levels assessed by oligonucleotide microarray analysis and quantitative real-time RT-PCR--how well do they correlate?[J]. BMC Genomics,2005,6(1):59-68.
[198]De Reynies A, Geromin D, Cayuela JM, et al. Comparison of the latest commercial short and long oligonucleotide microarray technologies[Jj]. BMC Genomics,2006,7:51-58.
[199]Morey J S, Ryan J C, Van Dolah F M. Microarray validation:factors influencing correlation between oligonucleotide microarrays and real-time PCR[J], Biological procedures online,2006, 8(1):175-193.