禽致病性大肠杆菌耐药基因和毒力因子的分子流行病学及HPI Irp1细胞表位作用的研究
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摘要
一、禽致病性大肠杆菌的分离鉴定及血清型分析
从江苏等5省不同地区送检的临床发病家禽,剖检可见肝周炎、心包炎、气囊炎、输卵管炎及卵黄性腹膜炎等病变,采集病料分离获得近500株细菌。经糖发酵试验、尿素酶试验、IMViC试验等生化试验以及三糖铁培养基培养试验,证实所获得的366株细菌为大肠杆菌。用大肠杆菌多因子血清和单因子血清进行血清型鉴定,对其中296株测出了血清型,覆盖了56种血清型,其中O78,O88,O24,O109,O93,O9,O11为优势血清型。分析结果发现禽源大肠杆菌的血清型变得日益复杂,在种类增加的基础上,与地区、宿主等亦有很大相关性:(1)不同地区来源的菌株优势血清型不同,南通分离株优势血清型为O78,O24,O11,O26,O45,O74;盐城株为O78,O87;扬州株为O93,O24,O88;常州菌株优势血清型为O78,O109;徐州菌株为O78,O86,O109,O1;泰州菌株为O86,O9。(2)不同家禽来源的菌株,血清型分布有较大差异,鸡、鸭、鹅源菌株血清型分别有41种、21种和11种,三者优势血清型不同,鸡源菌株为O78、O109、O88、O24,鹅源菌株为O9,O93,O88,O24四种血清型,鸭源大肠杆菌优势血清型为O78,尽管鹅源菌株只有11种血清型,但O114、O118、O123、O141、O23、O73等六种血清型却只出现于鹅源菌株,O128、O143两种血清型只出现于鸭源菌株。只有O11、O86、O107三种血清型在三种家禽分离株中均出现,这一现象说明,不同血清型菌株可能存在宿主特异性。
二、禽致病性大肠杆菌分离株耐药性检测
为了了解临床用药对细菌耐药性的影响,对346株禽致病性大肠杆菌分离菌株用圆纸片法进行了针对氨苄西林/舒巴坦、头孢噻肟、头孢曲松、庆大霉素、阿米卡星、链霉素、四环素、环丙沙星、恩诺沙星、诺氟沙星、复方新诺明、氯霉素、磷霉素、新霉素、利福平、痢特灵、壮观霉素、多黏菌B、阿奇霉素等19种
1. Isolation and identification of APEC and serotype
More than 500 bacterial strains were isolated from poultries, which had clinical lesions of colibacillosis with serious fibrin exudation, pericarditis, salpingitis, airsacculitis and yolk peritonitis. After cultured on MaConkey plates, single typical pink colonies were picked out after Gram-dyeing and purified on agar plates. After sugars ferment test, urea enzyme test, IMViC test and growth characteristic on TSI medium, 366 E.coli strains were identified. The serotype of APEC isolates were identified by the tube agglutination test with poly-serum and mono-serum. The serotype of 296 isolates were identified and grouped to 56 serotypes. O78,O88,O24,O109,O93,O9,O11 were main serotypes in this investigation. The results of the serotype test showed that the serotype of APEC became more complicate than ever. Beside the increasing of quantity of type, the serotype of APEC had relation to area of isolation and their hosts. (1)The isolates from different area had difference. The main serotypes of Nantong isolates were O78,O24,O11,O26,O45,O74;The Yancheng isolates were O78,O87;The Yangzhou isolates were O93,O24,O88;The Changzhou isolates were O78,O109;The Xuzhou isolates were O78,O86,O109,O1;The Taizhou isolates were O86,O9. (2) The isolates from different host had evident difference. The serotypes of isolates from chicks, ducks, geese were grouped to 41 serotypes, 21 serotypes and 11 serotypes respectively. The main serotype of isolates from chicken was O78, O109, O88, O24; The main serotype of isolates from duck was O78; The main serotype of isolates from goose was O9,O93,O88,O24. Only three serotypes O11, O86, O107 they all shared.
从江苏等5省不同地区送检的临床发病家禽,剖检可见肝周炎、心包炎、气囊炎、输卵管炎及卵黄性腹膜炎等病变,采集病料分离获得近500株细菌。经糖发酵试验、尿素酶试验、IMViC试验等生化试验以及三糖铁培养基培养试验,证实所获得的366株细菌为大肠杆菌。用大肠杆菌多因子血清和单因子血清进行血清型鉴定,对其中296株测出了血清型,覆盖了56种血清型,其中O78,O88,O24,O109,O93,O9,O11为优势血清型。分析结果发现禽源大肠杆菌的血清型变得日益复杂,在种类增加的基础上,与地区、宿主等亦有很大相关性:(1)不同地区来源的菌株优势血清型不同,南通分离株优势血清型为O78,O24,O11,O26,O45,O74;盐城株为O78,O87;扬州株为O93,O24,O88;常州菌株优势血清型为O78,O109;徐州菌株为O78,O86,O109,O1;泰州菌株为O86,O9。(2)不同家禽来源的菌株,血清型分布有较大差异,鸡、鸭、鹅源菌株血清型分别有41种、21种和11种,三者优势血清型不同,鸡源菌株为O78、O109、O88、O24,鹅源菌株为O9,O93,O88,O24四种血清型,鸭源大肠杆菌优势血清型为O78,尽管鹅源菌株只有11种血清型,但O114、O118、O123、O141、O23、O73等六种血清型却只出现于鹅源菌株,O128、O143两种血清型只出现于鸭源菌株。只有O11、O86、O107三种血清型在三种家禽分离株中均出现,这一现象说明,不同血清型菌株可能存在宿主特异性。
二、禽致病性大肠杆菌分离株耐药性检测
为了了解临床用药对细菌耐药性的影响,对346株禽致病性大肠杆菌分离菌株用圆纸片法进行了针对氨苄西林/舒巴坦、头孢噻肟、头孢曲松、庆大霉素、阿米卡星、链霉素、四环素、环丙沙星、恩诺沙星、诺氟沙星、复方新诺明、氯霉素、磷霉素、新霉素、利福平、痢特灵、壮观霉素、多黏菌B、阿奇霉素等19种
1. Isolation and identification of APEC and serotype
More than 500 bacterial strains were isolated from poultries, which had clinical lesions of colibacillosis with serious fibrin exudation, pericarditis, salpingitis, airsacculitis and yolk peritonitis. After cultured on MaConkey plates, single typical pink colonies were picked out after Gram-dyeing and purified on agar plates. After sugars ferment test, urea enzyme test, IMViC test and growth characteristic on TSI medium, 366 E.coli strains were identified. The serotype of APEC isolates were identified by the tube agglutination test with poly-serum and mono-serum. The serotype of 296 isolates were identified and grouped to 56 serotypes. O78,O88,O24,O109,O93,O9,O11 were main serotypes in this investigation. The results of the serotype test showed that the serotype of APEC became more complicate than ever. Beside the increasing of quantity of type, the serotype of APEC had relation to area of isolation and their hosts. (1)The isolates from different area had difference. The main serotypes of Nantong isolates were O78,O24,O11,O26,O45,O74;The Yancheng isolates were O78,O87;The Yangzhou isolates were O93,O24,O88;The Changzhou isolates were O78,O109;The Xuzhou isolates were O78,O86,O109,O1;The Taizhou isolates were O86,O9. (2) The isolates from different host had evident difference. The serotypes of isolates from chicks, ducks, geese were grouped to 41 serotypes, 21 serotypes and 11 serotypes respectively. The main serotype of isolates from chicken was O78, O109, O88, O24; The main serotype of isolates from duck was O78; The main serotype of isolates from goose was O9,O93,O88,O24. Only three serotypes O11, O86, O107 they all shared.
引文
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